pycom10g23540

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0017406
Physical Location & Seq
Forward (+)
25655852 .. 25656277
426 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23540.1

Sequence Viewer

Length: 426 bp
ATGACCAAGGCAAATGAGGCTGCTGCCTGGATAACTAGTTATGCAGAGGTACCTCCCAACAGTGAAACCGATCTACTCAAGGCTGTGTCCAAGCAACCAGTCTCCGTTTCCATCAATATATCAGAAGAATTTCAGCATTATTCAAGCGGGGTGTTCACCGGTGATGATTGTGGGACAGAAATGAGCCTCGCTGTTACCATCATTGGGTATGGGACAACTGAAGATGGCATCCCCTATTGGTTAATCAAGAAATCATGGTCCGATATGTGGGGTGAGACTGGCTACATGAAGATTCTTAGAAACGCTGATGTCCCAGGTGCTGTTTGTGGCCTTGCTACGAAAGCTTCCTATCCGATTATAAGTACTACAGACAAACCCGTAGGCTGTGAAAATGCCTGTATTTTGTACTTAAAGTATGATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

142

Amino Acids

15.32

Weight (kDa)

4.55

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 5 - 119 1.2e-31 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 359
Acc65I GGTACC 1 cut(s) 49
AccB1I GGYRCC 1 cut(s) 49
AciI CCGC 1 cut(s) 147
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 3 cut(s) 51, 364, 407
AfiI CCNNNNNNNGG 2 cut(s) 204, 267
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 1 cut(s) 144
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 2 cut(s) 26, 313
AluBI AGCT 1 cut(s) 344
AluI AGCT 1 cut(s) 344
Alw26I GTCTC 2 cut(s) 106, 269
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 1 cut(s) 328
ApeKI GCWGC 2 cut(s) 20, 23
ApoI RAATTY 1 cut(s) 128
AsiGI ACCGGT 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 129
Asp718I GGTACC 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 258
AsuHPI GGTGA 3 cut(s) 148, 173, 284
AvaII GGWCC 1 cut(s) 258
BanI GGYRCC 1 cut(s) 49
BbvI GCAGC 2 cut(s) 7, 10
BccI CCATC 3 cut(s) 119, 206, 218
BciT130I CCWGG 2 cut(s) 28, 315
BcoDI GTCTC 2 cut(s) 106, 269
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 366
BisI GCNGC 2 cut(s) 21, 24
BlsI GCNGC 2 cut(s) 22, 25
BmcAI AGTACT 1 cut(s) 364
Bme1390I CCNGG 2 cut(s) 28, 315
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 258
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 2 cut(s) 28, 315
BmsI GCATC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 6, 313
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 267
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 98, 283
BseBI CCWGG 2 cut(s) 28, 315
BseDI CCNNGG 2 cut(s) 6, 313
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 2 cut(s) 204, 267
BseNI ACTGG 2 cut(s) 98, 283
BseXI GCAGC 2 cut(s) 7, 10
BshFI GGCC 1 cut(s) 330
BshNI GGYRCC 1 cut(s) 49
BshTI ACCGGT 1 cut(s) 158
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 3 cut(s) 187, 226, 296
BslI CCNNNNNNNGG 2 cut(s) 204, 267
BsmAI GTCTC 2 cut(s) 106, 269
BsmFI GGGAC 3 cut(s) 187, 226, 296
BsnI GGCC 1 cut(s) 330
Bsp143I GATC 2 cut(s) 70, 418
BspACI CCGC 1 cut(s) 147
BspANI GGCC 1 cut(s) 330
BspLI GGNNCC 1 cut(s) 51
BspT107I GGYRCC 1 cut(s) 49
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 2 cut(s) 98, 283
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 6, 313
BssMI GATC 2 cut(s) 70, 418
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 28, 315
Bst4CI ACNGT 1 cut(s) 62
BstDEI CTNAG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 228
BstKTI GATC 2 cut(s) 73, 421
BstMAI GTCTC 2 cut(s) 106, 269
BstMBI GATC 2 cut(s) 70, 418
BstMWI GCNNNNNNNGC 2 cut(s) 17, 341
BstNI CCWGG 2 cut(s) 28, 315
BstSCI CCNGG 2 cut(s) 26, 313
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 7, 10
BsuRI GGCC 1 cut(s) 330
BtsCI GGATG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 67
CaiI CAGNNNCTG 1 cut(s) 320
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 258
Csp6I GTAC 3 cut(s) 50, 363, 406
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 255, 286
CviJI RGCY 7 cut(s) 20, 83, 186, 282, 330, 344, 384
CviKI_1 RGCY 7 cut(s) 20, 83, 186, 282, 330, 344, 384
CviQI GTAC 3 cut(s) 50, 363, 406
DdeI CTNAG 1 cut(s) 296
DpnI GATC 2 cut(s) 72, 420
DpnII GATC 2 cut(s) 70, 418
Eco130I CCWWGG 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 26, 313
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 2 cut(s) 258, 289
FaiI YATR 9 cut(s) 42, 119, 210, 256, 266, 287, 359, 417, 424
FaqI GGGAC 3 cut(s) 187, 226, 296
FatI CATG 2 cut(s) 254, 285
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 2 cut(s) 21, 24
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 21, 24
FspBI CTAG 1 cut(s) 36
GluI GCNGC 2 cut(s) 21, 24
HaeIII GGCC 1 cut(s) 330
HapII CCGG 1 cut(s) 159
Hin1II CATG 2 cut(s) 258, 289
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 1 cut(s) 292
HpaII CCGG 1 cut(s) 159
HphI GGTGA 3 cut(s) 148, 173, 284
Hpy166II GTNNAC 1 cut(s) 156
Hpy188I TCNGA 3 cut(s) 124, 262, 354
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 1 cut(s) 156
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 341
HpyF3I CTNAG 1 cut(s) 296
Hsp92II CATG 2 cut(s) 258, 289
KpnI GGTACC 1 cut(s) 53
Kzo9I GATC 2 cut(s) 70, 418
LpnPI CCDG 8 cut(s) 13, 40, 111, 172, 264, 300, 327, 409
Lsp1109I GCAGC 2 cut(s) 7, 10
LweI GCATC 1 cut(s) 237
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 193
MalI GATC 2 cut(s) 72, 420
MboI GATC 2 cut(s) 70, 418
MboII GAAGA 3 cut(s) 137, 233, 301
MluCI AATT 1 cut(s) 128
MnlI CCTC 4 cut(s) 10, 40, 63, 197
MroXI GAANNNNTTC 1 cut(s) 129
MseI TTAA 2 cut(s) 242, 410
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 2 cut(s) 28, 315
MvaI CCWGG 2 cut(s) 28, 315
MwoI GCNNNNNNNGC 2 cut(s) 17, 341
NdeII GATC 2 cut(s) 70, 418
NlaIII CATG 2 cut(s) 258, 289
NlaIV GGNNCC 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 129
PfeI GAWTC 1 cut(s) 292
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 2 cut(s) 22, 25
PsiI TTATAA 1 cut(s) 359
Psp6I CCWGG 2 cut(s) 26, 313
PspGI CCWGG 2 cut(s) 26, 313
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 1 cut(s) 258
PstNI CAGNNNCTG 1 cut(s) 320
RsaI GTAC 3 cut(s) 51, 364, 407
RsaNI GTAC 3 cut(s) 50, 363, 406
SaqAI TTAA 2 cut(s) 242, 410
SatI GCNGC 2 cut(s) 21, 24
Sau3AI GATC 2 cut(s) 70, 418
Sau96I GGNCC 1 cut(s) 258
ScaI AGTACT 1 cut(s) 364
ScrFI CCNGG 2 cut(s) 28, 315
SetI ASST 4 cut(s) 51, 55, 319, 346
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 366
SgrAI CRCCGGYG 1 cut(s) 158
SinI GGWCC 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 1 cut(s) 128
SsiI CCGC 1 cut(s) 147
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 2 cut(s) 26, 313
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 62
TasI AATT 1 cut(s) 128
TatI WGTACW 2 cut(s) 362, 405
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 2 cut(s) 242, 410
Tru9I TTAA 2 cut(s) 242, 410
TscAI CASTG 1 cut(s) 67
TseI GCWGC 2 cut(s) 20, 23
TspDTI ATGAA 1 cut(s) 302
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 67
VpaK11BI GGWCC 1 cut(s) 258
XapI RAATTY 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 1 cut(s) 36
ZrmI AGTACT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.