Rh5BG078000

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017406
Physical Location & Seq
Forward (+)
6604915 .. 6622940
18026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG078000.1

Sequence Viewer

Length: 825 bp
ATGACTTTCCCATTATATGGTATGCTAGTTCTTTCGGGGGCTACTTGGGGGTCTACGGGTGCTCAATCCAAGACTGCGACTACGTTGAATGAAGCTTCCCTTGCTACAAAACATGAGCTATGGATGGCTCGGTATGAACGTGTATACGCAGACAACGCAGAAAAGGAGAGACGTTTCAAGATATTCAAGGACAATGTGGAATACGTAGACAAGTTCAACGATGAAGAGAATAATCACACTTACAAGTTAAGCCTCAACGAATTTGCAGACTTAACCGATGAAGAATTCTCGAGGGCTTATACTGGGTGCAAGAGGTCCATTCGTTCAGCAGGATCATTTAAAACGATCGACCCTATCAAGAAGTTGCTGGGCGTTTTCAGCAGTGGCAGCTGTGGAGGGGATTACGAAAATCAAATCCGCTATGGTCACCTGGACAGTGCCTTTGCTTACATAGCACATGCCGGCATTGCCAGTGAATCAAATTACCCATACACAGAAAAGGAAGGAACTTGTGATGCTAACAAGACAAGGGACTATGCTGCCAAGATCACAAGCTATGCCACCGTTGATCTTAACAGCGAGACAGAATTATTAAATGTGGTTTTTAAGCAACCGGTCTCAGCAATGGTTGACAGCAAAGGGAGGGAGTTCAGACTTTACTCCGGTGGAGTCTTCTCTGGAGAATGTGGGACATCACTGGACCATTCTGTAACGGTCATCGGGTATGGGACGACTGAGGATGGCATATGGGGTGAGCACTGGGGAGAGAGTGGCTACATGAGAATCCGCAGAGGGGTTGATGCTCCACAAGGTCCCGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0000325 GO:0000932 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005788 GO:0005911 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010494 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030054 GO:0030154 GO:0030163 GO:0030312 GO:0031974 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034050 GO:0035770 GO:0036464 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0048046 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0061458 GO:0070011 GO:0070013 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1 Propeptide_C1

Protein Analysis

274

Amino Acids

30.47

Weight (kDa)

5.27

Isoelectric Point (pI)

30.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 38 - 96 4.2e-19 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 131 - 267 2.1e-29 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 17
AccI GTMKAC 3 cut(s) 53, 144, 207
AciI CCGC 2 cut(s) 418, 787
AclWI GGATC 1 cut(s) 340
AcsI RAATTY 3 cut(s) 260, 284, 819
AfiI CCNNNNNNNGG 2 cut(s) 17, 793
AflIII ACRYGT 1 cut(s) 139
AgeI ACCGGT 1 cut(s) 613
AgsI TTSAA 4 cut(s) 88, 178, 187, 217
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 4 cut(s) 95, 118, 390, 555
AluI AGCT 4 cut(s) 95, 118, 390, 555
Alw21I GWGCWC 2 cut(s) 64, 759
Alw26I GTCTC 3 cut(s) 163, 575, 622
AlwI GGATC 1 cut(s) 340
Ama87I CYCGRG 1 cut(s) 289
ApeKI GCWGC 2 cut(s) 387, 539
ApoI RAATTY 3 cut(s) 260, 284, 819
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AsiGI ACCGGT 1 cut(s) 613
AspS9I GGNCC 3 cut(s) 315, 700, 812
AsuHPI GGTGA 2 cut(s) 419, 764
AvaI CYCGRG 1 cut(s) 289
AvaII GGWCC 3 cut(s) 315, 700, 812
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 2 cut(s) 64, 759
BbvI GCAGC 2 cut(s) 399, 526
BccI CCATC 2 cut(s) 118, 734
BciT130I CCWGG 1 cut(s) 431
BcoDI GTCTC 3 cut(s) 163, 575, 622
BfaI CTAG 1 cut(s) 26
BisI GCNGC 2 cut(s) 388, 540
BlsI GCNGC 2 cut(s) 389, 541
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 3 cut(s) 315, 700, 812
BmeT110I CYCGRG 1 cut(s) 289
BmgT120I GGNCC 3 cut(s) 315, 700, 812
BmiI GGNNCC 1 cut(s) 814
BmrFI CCNGG 1 cut(s) 431
BmrI ACTGGG 2 cut(s) 312, 769
BmsI GCATC 2 cut(s) 505, 790
BmuI ACTGGG 2 cut(s) 312, 769
BpiI GAAGAC 1 cut(s) 664
BpmI CTGGAG 1 cut(s) 699
BsaAI YACGTR 1 cut(s) 205
BsaI GGTCTC 1 cut(s) 622
BsaWI WCCGGW 2 cut(s) 613, 662
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 793
Bse118I RCCGGY 2 cut(s) 461, 613
Bse1I ACTGG 4 cut(s) 307, 471, 702, 764
Bse3DI GCAATG 2 cut(s) 465, 630
BseBI CCWGG 1 cut(s) 431
BseGI GGATG 2 cut(s) 129, 745
BseLI CCNNNNNNNGG 2 cut(s) 17, 793
BseMI GCAATG 2 cut(s) 465, 630
BseMII CTCAG 2 cut(s) 633, 726
BseNI ACTGG 4 cut(s) 307, 471, 702, 764
BseXI GCAGC 2 cut(s) 399, 526
BseYI CCCAGC 1 cut(s) 367
Bsh1285I CGRYCG 1 cut(s) 348
BshTI ACCGGT 1 cut(s) 613
BsiEI CGRYCG 1 cut(s) 348
BsiHKAI GWGCWC 2 cut(s) 64, 759
BsiHKCI CYCGRG 1 cut(s) 289
BsiSI CCGG 3 cut(s) 462, 614, 663
BslFI GGGAC 4 cut(s) 545, 703, 742, 798
BslI CCNNNNNNNGG 2 cut(s) 17, 793
BsmAI GTCTC 3 cut(s) 163, 575, 622
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 4 cut(s) 545, 703, 742, 798
Bso31I GGTCTC 1 cut(s) 622
BsoBI CYCGRG 1 cut(s) 289
Bsp1286I GDGCHC 2 cut(s) 64, 759
Bsp143I GATC 4 cut(s) 332, 345, 546, 568
BspACI CCGC 2 cut(s) 418, 787
BspCNI CTCAG 2 cut(s) 632, 727
BspLI GGNNCC 1 cut(s) 814
BspPI GGATC 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 622
BsrDI GCAATG 2 cut(s) 465, 630
BsrFI RCCGGY 2 cut(s) 461, 613
BsrI ACTGG 4 cut(s) 307, 471, 702, 764
BssAI RCCGGY 2 cut(s) 461, 613
BssMI GATC 4 cut(s) 332, 345, 546, 568
BssNAI GTATAC 1 cut(s) 145
Bst1107I GTATAC 1 cut(s) 145
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 3 cut(s) 437, 565, 715
Bst6I CTCTTC 1 cut(s) 219
BstBAI YACGTR 1 cut(s) 205
BstC8I GCNNGC 1 cut(s) 463
BstDEI CTNAG 2 cut(s) 619, 735
BstEII GGTNACC 1 cut(s) 425
BstF5I GGATG 2 cut(s) 129, 745
BstKTI GATC 4 cut(s) 335, 348, 549, 571
BstMAI GTCTC 3 cut(s) 163, 575, 622
BstMBI GATC 4 cut(s) 332, 345, 546, 568
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 6 cut(s) 101, 155, 378, 387, 452, 467
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 1 cut(s) 461
BstPI GGTNACC 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 429
BstSNI TACGTA 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 399, 526
BstV2I GAAGAC 1 cut(s) 664
BstZ17I GTATAC 1 cut(s) 145
BtsCI GGATG 2 cut(s) 129, 745
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 5 cut(s) 388, 442, 478, 695, 757
Cac8I GCNNGC 1 cut(s) 463
Cfr10I RCCGGY 2 cut(s) 461, 613
Cfr13I GGNCC 3 cut(s) 315, 700, 812
CspAI ACCGGT 1 cut(s) 613
CviAII CATG 3 cut(s) 113, 458, 778
CviJI RGCY 9 cut(s) 41, 95, 118, 128, 252, 296, 390, 555, 774
CviKI_1 RGCY 9 cut(s) 41, 95, 118, 128, 252, 296, 390, 555, 774
DdeI CTNAG 2 cut(s) 619, 735
DpnI GATC 4 cut(s) 334, 347, 548, 570
DpnII GATC 4 cut(s) 332, 345, 546, 568
DraI TTTAAA 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco105I TACGTA 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 622
Eco47I GGWCC 3 cut(s) 315, 700, 812
Eco88I CYCGRG 1 cut(s) 289
Eco91I GGTNACC 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 812
EcoO65I GGTNACC 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 429
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 3 cut(s) 116, 461, 781
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 4 cut(s) 545, 703, 742, 798
FatI CATG 3 cut(s) 112, 457, 777
FauNDI CATATG 1 cut(s) 746
FblI GTMKAC 3 cut(s) 53, 144, 207
Fnu4HI GCNGC 2 cut(s) 388, 540
FokI GGATG 2 cut(s) 136, 752
Fsp4HI GCNGC 2 cut(s) 388, 540
FspBI CTAG 1 cut(s) 26
GluI GCNGC 2 cut(s) 388, 540
GsaI CCCAGC 1 cut(s) 371
GsuI CTGGAG 1 cut(s) 699
HapII CCGG 3 cut(s) 462, 614, 663
Hin1II CATG 3 cut(s) 116, 461, 781
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 3 cut(s) 476, 669, 783
HpaII CCGG 3 cut(s) 462, 614, 663
HphI GGTGA 2 cut(s) 419, 764
Hpy166II GTNNAC 4 cut(s) 54, 145, 208, 631
Hpy188I TCNGA 1 cut(s) 653
Hpy188III TCNNGA 5 cut(s) 178, 289, 358, 678, 815
Hpy8I GTNNAC 4 cut(s) 54, 145, 208, 631
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 3 cut(s) 437, 565, 715
HpyCH4IV ACGT 4 cut(s) 83, 139, 172, 204
HpyCH4V TGCA 2 cut(s) 266, 309
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 155, 378, 387, 452, 467
HpyF3I CTNAG 2 cut(s) 619, 735
HpySE526I ACGT 4 cut(s) 83, 139, 172, 204
Hsp92II CATG 3 cut(s) 116, 461, 781
KroI GCCGGC 1 cut(s) 461
KroNI GCCGGC 1 cut(s) 463
Kzo9I GATC 4 cut(s) 332, 345, 546, 568
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 2 cut(s) 399, 526
LweI GCATC 2 cut(s) 505, 790
MaeI CTAG 1 cut(s) 26
MaeII ACGT 4 cut(s) 83, 139, 172, 204
MaeIII GTNAC 2 cut(s) 425, 709
MalI GATC 4 cut(s) 334, 347, 548, 570
MboI GATC 4 cut(s) 332, 345, 546, 568
MboII GAAGA 3 cut(s) 236, 293, 664
MhlI GDGCHC 2 cut(s) 64, 759
MluCI AATT 5 cut(s) 260, 284, 481, 587, 819
MlyI GAGTC 1 cut(s) 678
MnlI CCTC 7 cut(s) 263, 285, 306, 389, 636, 730, 785
MroNI GCCGGC 1 cut(s) 461
MseI TTAA 6 cut(s) 248, 272, 339, 573, 593, 606
MspA1I CMGCKG 1 cut(s) 390
MspI CCGG 3 cut(s) 462, 614, 663
MspR9I CCNGG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 431
MwoI GCNNNNNNNGC 6 cut(s) 101, 155, 378, 387, 452, 467
NaeI GCCGGC 1 cut(s) 463
NdeI CATATG 1 cut(s) 746
NdeII GATC 4 cut(s) 332, 345, 546, 568
NgoMIV GCCGGC 1 cut(s) 461
NlaIII CATG 3 cut(s) 116, 461, 781
NlaIV GGNNCC 1 cut(s) 814
NmuCI GTSAC 1 cut(s) 425
NspI RCATGY 1 cut(s) 461
PaeR7I CTCGAG 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 136
PdiI GCCGGC 1 cut(s) 463
PfeI GAWTC 2 cut(s) 476, 783
PflMI CCANNNNNTGG 1 cut(s) 17
PinAI ACCGGT 1 cut(s) 613
PkrI GCNGC 2 cut(s) 389, 541
Ple19I CGATCG 1 cut(s) 348
PleI GAGTC 1 cut(s) 677
PpsI GAGTC 1 cut(s) 677
Ppu21I YACGTR 1 cut(s) 205
PpuMI RGGWCCY 1 cut(s) 812
Psp5II RGGWCCY 1 cut(s) 812
Psp6I CCWGG 1 cut(s) 429
PspEI GGTNACC 1 cut(s) 425
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 814
PspPI GGNCC 3 cut(s) 315, 700, 812
PspPPI RGGWCCY 1 cut(s) 812
PvuI CGATCG 1 cut(s) 348
PvuII CAGCTG 1 cut(s) 390
SaqAI TTAA 6 cut(s) 248, 272, 339, 573, 593, 606
SatI GCNGC 2 cut(s) 388, 540
Sau3AI GATC 4 cut(s) 332, 345, 546, 568
Sau96I GGNCC 3 cut(s) 315, 700, 812
SchI GAGTC 1 cut(s) 678
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 2 cut(s) 64, 759
SfaNI GCATC 2 cut(s) 505, 790
Sfr274I CTCGAG 1 cut(s) 289
SinI GGWCC 3 cut(s) 315, 700, 812
SlaI CTCGAG 1 cut(s) 289
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
SnaBI TACGTA 1 cut(s) 205
Sse9I AATT 5 cut(s) 260, 284, 481, 587, 819
SsiI CCGC 2 cut(s) 418, 787
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 429
TaaI ACNGT 3 cut(s) 437, 565, 715
TaiI ACGT 4 cut(s) 86, 142, 175, 207
TaqI TCGA 2 cut(s) 290, 348
TasI AATT 5 cut(s) 260, 284, 481, 587, 819
TfiI GAWTC 2 cut(s) 476, 783
Tru1I TTAA 6 cut(s) 248, 272, 339, 573, 593, 606
Tru9I TTAA 6 cut(s) 248, 272, 339, 573, 593, 606
TscAI CASTG 5 cut(s) 388, 442, 478, 702, 764
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 2 cut(s) 387, 539
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 4 cut(s) 105, 150, 237, 294
TspRI CASTG 5 cut(s) 388, 442, 478, 702, 764
Van91I CCANNNNNTGG 1 cut(s) 17
VpaK11BI GGWCC 3 cut(s) 315, 700, 812
XapI RAATTY 3 cut(s) 260, 284, 819
XceI RCATGY 1 cut(s) 461
XhoI CTCGAG 1 cut(s) 289
XmiI GTMKAC 3 cut(s) 53, 144, 207
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.