pycom10g23880

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016036
Physical Location & Seq
Forward (+)
25851885 .. 25853596
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1083 bp
ATGCATTCAAAAATGGTGGCGATGGGTGTGCTGATCGTAACGTTGGGTTTGCTGCTTCCTTTGCTCTCAGGAGCAGTCGACATTGGCCGAGTGAGGGAAGTCGCTGCTCGGAACAACGTGACATGTGTCTTAGTGTTTGGAGATTCAAGTGTTGATCCTGGCAACAACAATGTTCTACCGACCTTCCTGAAGGGCAACTTCCTTCCTTATGGGAAAGACTTCTTCAATGGCCTCCCTACTGGGAGGTTCTCTAATGGAAGACTTGCCACAGATTTTATTGCGGATGCCCTTGGCTACACAAAAATAATCCCAGCATTTCTTGACACCAATTTGAAGTTGCAAGATCTGCCGCATGGTGTTAGTTTCGCATCAGCTGGTTCCGGCTATGATGATTTGACCGCCAACATTTCGAGTGTATTGTCTCTTCCCAGACAGCTGAAGTATTTTTTGCATTACAAAGTCAAGCTGATAAAATTGGTAGGTCAGAAGAAGGCTGAGGATATTATTAGAACTGCTGTACTCGTATTGAGTATGGGCACAAATGACTTTGTCCAAAATTACTACTTGGACCCAACTCGACCCAAGCAGTTCAGTGTTGAAGAGTACCAAAATTACTTGGTCTCTTGCATGGCTAATGGCATTCAGCAAATGGAGAGGATAGGAGCGACAAGATTGGTTGTGGTTGGGGTTCCACCATTGGGGTGCATGCCACTAGTGAAAACGCTAATGGACGTGAGAGCATGTGCTGAAAATTACAACAAAGTGGCATCCTCGTTGGGTTCCAGGATACAAAAGAAATTGGAGGCCATAACCAAAACATCCAGAATGAAAATTGGTTTTGTTGATGCTTATGGTATTATACAAAATGCCATAAATAACCCAAAATTATATGATTTGGCTGAAACTTCAAAAGGATGCTGCGGGACAGGAGCCATAGAATTTGGAGATTCATGCCGGGGACTAGAGACATGCACTGACCCAGCAAAGTACGTATACTGGGATGCTGTTCATCCAACTGAAAAAATGTACAAAATCATTGCTGACAAGGCTCTCGAAACTCTTTCCGAAGCACTCTTCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

361

Amino Acids

39.36

Weight (kDa)

8.64

Isoelectric Point (pI)

26.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 349 5.6e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 78, 993
AciI CCGC 4 cut(s) 281, 350, 399, 921
AclI AACGTT 1 cut(s) 41
AclWI GGATC 1 cut(s) 149
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 1 cut(s) 938
AcuI CTGAAG 2 cut(s) 209, 458
AfaI GTAC 4 cut(s) 519, 605, 989, 1028
AfiI CCNNNNNNNGG 2 cut(s) 94, 698
AflIII ACRYGT 1 cut(s) 122
AgsI TTSAA 6 cut(s) 9, 147, 226, 334, 599, 909
AhlI ACTAGT 1 cut(s) 712
AjiI CACGTC 1 cut(s) 733
AjnI CCWGG 2 cut(s) 157, 782
AluBI AGCT 3 cut(s) 374, 436, 466
AluI AGCT 3 cut(s) 374, 436, 466
Alw26I GTCTC 3 cut(s) 426, 625, 959
AlwI GGATC 1 cut(s) 149
AoxI GGCC 3 cut(s) 85, 229, 804
ApeKI GCWGC 3 cut(s) 52, 104, 918
ApoI RAATTY 1 cut(s) 938
Asp700I GAANNNNTTC 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 568
AsuC2I CCSGG 1 cut(s) 956
AvaII GGWCC 1 cut(s) 568
BaeGI GKGCMC 1 cut(s) 539
BarI GAAGNNNNNNTAC 2 cut(s) 408, 440
BbsI GAAGAC 1 cut(s) 265
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 3 cut(s) 39, 91, 905
BccI CCATC 1 cut(s) 16
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 2 cut(s) 159, 784
BciVI GTATCC 1 cut(s) 780
BcnI CCSGG 1 cut(s) 956
BcoDI GTCTC 3 cut(s) 426, 625, 959
BcuI ACTAGT 1 cut(s) 712
BfaI CTAG 2 cut(s) 713, 962
BfuI GTATCC 1 cut(s) 780
BglII AGATCT 1 cut(s) 343
BisI GCNGC 4 cut(s) 53, 105, 350, 919
BlsI GCNGC 4 cut(s) 54, 106, 351, 920
Bme1390I CCNGG 3 cut(s) 159, 784, 956
Bme18I GGWCC 1 cut(s) 568
BmgBI CACGTC 1 cut(s) 733
BmgT120I GGNCC 1 cut(s) 568
BmiI GGNNCC 5 cut(s) 379, 570, 690, 781, 931
BmrFI CCNGG 3 cut(s) 159, 784, 956
BmrI ACTGGG 2 cut(s) 249, 1006
BmsI GCATC 6 cut(s) 274, 377, 776, 835, 905, 991
BmuI ACTGGG 2 cut(s) 249, 1006
BoxI GACNNNNGTC 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 265
Bpu10I CCTNAGC 1 cut(s) 495
BpuMI CCSGG 1 cut(s) 956
BsaAI YACGTR 1 cut(s) 991
BsaI GGTCTC 1 cut(s) 625
BsaJI CCNNGG 2 cut(s) 289, 955
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 698
Bse1I ACTGG 2 cut(s) 244, 1001
Bse3DI GCAATG 1 cut(s) 1035
BseBI CCWGG 2 cut(s) 159, 784
BseDI CCNNGG 2 cut(s) 289, 955
BseGI GGATG 6 cut(s) 289, 767, 818, 920, 1006, 1009
BseLI CCNNNNNNNGG 2 cut(s) 94, 698
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 2 cut(s) 81, 486
BseNI ACTGG 2 cut(s) 244, 1001
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 3 cut(s) 39, 91, 905
BseYI CCCAGC 2 cut(s) 310, 979
BshFI GGCC 3 cut(s) 87, 231, 806
BsiSI CCGG 2 cut(s) 381, 955
BslFI GGGAC 2 cut(s) 937, 972
BslI CCNNNNNNNGG 2 cut(s) 94, 698
BsmAI GTCTC 3 cut(s) 426, 625, 959
BsmFI GGGAC 2 cut(s) 937, 972
BsmI GAATGC 2 cut(s) 4, 639
BsnI GGCC 3 cut(s) 87, 231, 806
Bso31I GGTCTC 1 cut(s) 625
Bsp1286I GDGCHC 1 cut(s) 539
Bsp1407I TGTACA 1 cut(s) 1026
Bsp143I GATC 3 cut(s) 33, 154, 343
BspACI CCGC 4 cut(s) 281, 350, 399, 921
BspANI GGCC 3 cut(s) 87, 231, 806
BspCNI CTCAG 2 cut(s) 80, 487
BspLI GGNNCC 5 cut(s) 379, 570, 690, 781, 931
BspPI GGATC 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 625
BsrDI GCAATG 1 cut(s) 1035
BsrGI TGTACA 1 cut(s) 1026
BsrI ACTGG 2 cut(s) 244, 1001
BssECI CCNNGG 2 cut(s) 289, 955
BssMI GATC 3 cut(s) 33, 154, 343
BssNAI GTATAC 1 cut(s) 994
BssT1I CCWWGG 1 cut(s) 289
Bst1107I GTATAC 1 cut(s) 994
Bst2UI CCWGG 2 cut(s) 159, 784
Bst6I CTCTTC 3 cut(s) 429, 594, 1079
BstAPI GCANNNNNTGC 1 cut(s) 346
BstAUI TGTACA 1 cut(s) 1026
BstBAI YACGTR 1 cut(s) 991
BstC8I GCNNGC 1 cut(s) 707
BstDEI CTNAG 3 cut(s) 67, 130, 495
BstF5I GGATG 6 cut(s) 289, 767, 818, 920, 1006, 1009
BstKTI GATC 3 cut(s) 36, 157, 346
BstMAI GTCTC 3 cut(s) 426, 625, 959
BstMBI GATC 3 cut(s) 33, 154, 343
BstMWI GCNNNNNNNGC 3 cut(s) 61, 346, 1046
BstNI CCWGG 2 cut(s) 159, 784
BstNSI RCATGY 4 cut(s) 126, 709, 744, 972
BstPAI GACNNNNGTC 1 cut(s) 125
BstSCI CCNGG 3 cut(s) 157, 782, 954
BstSLI GKGCMC 1 cut(s) 539
BstSNI TACGTA 1 cut(s) 991
BstV1I GCAGC 3 cut(s) 39, 91, 905
BstV2I GAAGAC 1 cut(s) 265
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BstZ17I GTATAC 1 cut(s) 994
BsuI GTATCC 1 cut(s) 780
BsuRI GGCC 3 cut(s) 87, 231, 806
BtgZI GCGATG 1 cut(s) 35
BtrI CACGTC 1 cut(s) 733
BtsCI GGATG 6 cut(s) 289, 767, 818, 920, 1006, 1009
BtsIMutI CAGTG 2 cut(s) 598, 972
Cac8I GCNNGC 1 cut(s) 707
Cfr13I GGNCC 1 cut(s) 568
Csp6I GTAC 4 cut(s) 518, 604, 988, 1027
CspCI CAANNNNNGTGG 1 cut(s) 32
CviAII CATG 7 cut(s) 123, 353, 628, 706, 741, 951, 969
CviQI GTAC 4 cut(s) 518, 604, 988, 1027
DdeI CTNAG 3 cut(s) 67, 130, 495
DpnI GATC 3 cut(s) 35, 156, 345
DpnII GATC 3 cut(s) 33, 154, 343
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 3 cut(s) 429, 594, 1079
EarI CTCTTC 3 cut(s) 429, 594, 1079
Eco105I TACGTA 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 289
Eco31I GGTCTC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 568
Eco57I CTGAAG 2 cut(s) 209, 458
EcoRII CCWGG 2 cut(s) 157, 782
EcoT14I CCWWGG 1 cut(s) 289
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 289
FaeI CATG 7 cut(s) 126, 356, 631, 709, 744, 954, 972
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 2 cut(s) 937, 972
FatI CATG 7 cut(s) 122, 352, 627, 705, 740, 950, 968
FauI CCCGC 1 cut(s) 914
FblI GTMKAC 2 cut(s) 78, 993
Fnu4HI GCNGC 4 cut(s) 53, 105, 350, 919
FokI GGATG 6 cut(s) 296, 754, 805, 927, 996, 1013
Fsp4HI GCNGC 4 cut(s) 53, 105, 350, 919
FspBI CTAG 2 cut(s) 713, 962
GluI GCNGC 4 cut(s) 53, 105, 350, 919
GsaI CCCAGC 2 cut(s) 314, 983
HaeIII GGCC 3 cut(s) 87, 231, 806
HapII CCGG 2 cut(s) 381, 955
Hin1II CATG 7 cut(s) 126, 356, 631, 709, 744, 954, 972
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 2 cut(s) 143, 947
HpaII CCGG 2 cut(s) 381, 955
Hpy166II GTNNAC 2 cut(s) 79, 994
Hpy188I TCNGA 3 cut(s) 111, 486, 1066
Hpy188III TCNNGA 5 cut(s) 69, 187, 320, 822, 1052
Hpy8I GTNNAC 2 cut(s) 79, 994
HpyAV CCTTC 4 cut(s) 184, 193, 212, 484
HpyCH4IV ACGT 4 cut(s) 41, 117, 732, 990
HpyCH4V TGCA 6 cut(s) 4, 340, 451, 627, 705, 972
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 346, 1046
HpyF3I CTNAG 3 cut(s) 67, 130, 495
HpySE526I ACGT 4 cut(s) 41, 117, 732, 990
Hsp92II CATG 7 cut(s) 126, 356, 631, 709, 744, 954, 972
Kzo9I GATC 3 cut(s) 33, 154, 343
LmnI GCTCC 3 cut(s) 71, 662, 929
Lsp1109I GCAGC 3 cut(s) 39, 91, 905
LweI GCATC 6 cut(s) 274, 377, 776, 835, 905, 991
MaeI CTAG 2 cut(s) 713, 962
MaeII ACGT 4 cut(s) 41, 117, 732, 990
MaeIII GTNAC 2 cut(s) 37, 118
MalI GATC 3 cut(s) 35, 156, 345
MboI GATC 3 cut(s) 33, 154, 343
MboII GAAGA 6 cut(s) 214, 270, 416, 499, 611, 1066
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 9 cut(s) 328, 473, 556, 610, 751, 797, 831, 884, 938
MmeI TCCRAC 1 cut(s) 1037
MnlI CCTC 7 cut(s) 87, 237, 242, 490, 648, 781, 796
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 218
MslI CAYNNNNRTG 1 cut(s) 700
MspA1I CMGCKG 2 cut(s) 374, 436
MspI CCGG 2 cut(s) 381, 955
MspR9I CCNGG 3 cut(s) 159, 784, 956
Mva1269I GAATGC 2 cut(s) 4, 639
MvaI CCWGG 2 cut(s) 159, 784
MwoI GCNNNNNNNGC 3 cut(s) 61, 346, 1046
NciI CCSGG 1 cut(s) 956
NdeII GATC 3 cut(s) 33, 154, 343
NlaIII CATG 7 cut(s) 126, 356, 631, 709, 744, 954, 972
NlaIV GGNNCC 5 cut(s) 379, 570, 690, 781, 931
NmeAIII GCCGAG 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 118
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 4 cut(s) 126, 709, 744, 972
PaeI GCATGC 1 cut(s) 709
PciI ACATGT 1 cut(s) 122
PctI GAATGC 2 cut(s) 4, 639
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 143, 947
PflFI GACNNNGTC 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 782
PkrI GCNGC 4 cut(s) 54, 106, 351, 920
Ppu21I YACGTR 1 cut(s) 991
PscI ACATGT 1 cut(s) 122
PshAI GACNNNNGTC 1 cut(s) 125
Psp1406I AACGTT 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 157, 782
PspFI CCCAGC 2 cut(s) 310, 979
PspGI CCWGG 2 cut(s) 157, 782
PspN4I GGNNCC 5 cut(s) 379, 570, 690, 781, 931
PspPI GGNCC 1 cut(s) 568
PsuI RGATCY 1 cut(s) 343
PsyI GACNNNGTC 1 cut(s) 548
PvuII CAGCTG 2 cut(s) 374, 436
RsaI GTAC 4 cut(s) 519, 605, 989, 1028
RsaNI GTAC 4 cut(s) 518, 604, 988, 1027
RseI CAYNNNNRTG 1 cut(s) 700
SalI GTCGAC 1 cut(s) 77
SatI GCNGC 4 cut(s) 53, 105, 350, 919
Sau3AI GATC 3 cut(s) 33, 154, 343
Sau96I GGNCC 1 cut(s) 568
ScrFI CCNGG 3 cut(s) 159, 784, 956
SduI GDGCHC 1 cut(s) 539
SfaNI GCATC 6 cut(s) 274, 377, 776, 835, 905, 991
SinI GGWCC 1 cut(s) 568
SmiMI CAYNNNNRTG 1 cut(s) 700
SnaBI TACGTA 1 cut(s) 991
SpeI ACTAGT 1 cut(s) 712
SphI GCATGC 1 cut(s) 709
Sse9I AATT 9 cut(s) 328, 473, 556, 610, 751, 797, 831, 884, 938
SsiI CCGC 4 cut(s) 281, 350, 399, 921
SspMI CTAG 2 cut(s) 713, 962
StyD4I CCNGG 3 cut(s) 157, 782, 954
StyI CCWWGG 1 cut(s) 289
TaiI ACGT 4 cut(s) 44, 120, 735, 993
TaqI TCGA 4 cut(s) 78, 410, 577, 1053
TasI AATT 9 cut(s) 328, 473, 556, 610, 751, 797, 831, 884, 938
TatI WGTACW 2 cut(s) 517, 1026
TauI GCSGC 1 cut(s) 352
TfiI GAWTC 2 cut(s) 143, 947
TscAI CASTG 2 cut(s) 598, 979
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 3 cut(s) 52, 104, 918
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 3 cut(s) 842, 939, 998
TspRI CASTG 2 cut(s) 598, 979
Tth111I GACNNNGTC 1 cut(s) 548
VpaK11BI GGWCC 1 cut(s) 568
XapI RAATTY 1 cut(s) 938
XceI RCATGY 4 cut(s) 126, 709, 744, 972
XmiI GTMKAC 2 cut(s) 78, 993
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 2 cut(s) 713, 962
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.