pycom10g25650
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001349
Physical Location & Seq
Forward (+)
27212036 .. 27213921
1886 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ATGCCTGCTGTTGGTATCGCCATCGGCGGGAACGGCAAGGCCTATGCCGGAAATCTCACTCCTTACGTTCTTGCCACATGTATCGTTGCAGCCATGGGTGGTCTGATTTTTGGCTACGATATCGGAATTTCTGGTGGTGTAACGTCCATGGATTCGTTCCTGAAGAAGTTCTTTCTGTCTGTGTATCACAAGAAGGAGAAAGACAAGACGAACAACCAGTACTGTCAGTATGATAGTCAAACGTTGACCATGTTCACATCGTCGCTGTACTTGGCTGCTCTCATATCCTCGATCGTGGCCGCCACCGTGACGAGAAAATTCGGCCGGAAACTGTCCATGTTGTTCGGCGGTCTGCTGTTTTGCTCCGGTGCTATCATCAATGCCGCTGCCAAAGCTGTCTGGATGTTGATCCTCGGCCGTATGTTGCTCGGTTTCGGCATCGGATTCTCCAATCAGTCAGTGCCACTCTACCTCTCTGAGATGGCACCCTACAAATTCAGGGGAGCTCTCAACATTGGCTTCCAGCTATCAATCACAATTGGTATCCTTGTGGCCAATGTGCTGAACTACTTCTTTGCCAAGAGATCGATCAAGGGTGGCTGGGGATGGAGATTGAGCTTGGGCGGCGCGATGGTGCCCGCCCTTATCATCACAATCGGCTCACTTGTCCTGCCAGACACTCCCAACTCCATGATTGAGCGCGGCCAGCATGACGAAGCCAAGACCAAGCTTCAGCGCATTCGCGGCGTTGATGATGTTAGCGAGGAGTTCAGTGACCTAGTTGCCGCCAGTGATGCCGCGAACCAAATTGAGGACCCGTGGAGAAACTTGTTGAGGAGGAAGTACAGACCTCACCTATGCATGGCAATCCTCATTCCCTTCTTTCAGCAGCTCACCGGCATTAATGTGATCGATGTTCTACGCGCCGGTTTTGTTCAACACGATCGGGTTTGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

320

Amino Acids

34.9

Weight (kDa)

9.53

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 27 - 305 1.8e-77 Sugar (and other) transporter
MFS_1 PF07690 77 - 295 2.9e-17 Major Facilitator Superfamily
TRI12 PF06609 82 - 207 4e-06 Fungal trichothecene efflux pump (TRI12)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 484, 634
AccII CGCG 5 cut(s) 629, 702, 744, 800, 924
AclI AACGTT 1 cut(s) 242
AclWI GGATC 1 cut(s) 403
AcoI YGGCCR 5 cut(s) 297, 322, 415, 552, 703
AcsI RAATTY 3 cut(s) 126, 317, 494
AcuI CTGAAG 2 cut(s) 182, 716
AfaI GTAC 3 cut(s) 221, 269, 845
AfiI CCNNNNNNNGG 4 cut(s) 11, 27, 811, 862
AflIII ACRYGT 1 cut(s) 77
AgsI TTSAA 1 cut(s) 938
AluBI AGCT 6 cut(s) 395, 506, 526, 618, 730, 892
AluI AGCT 6 cut(s) 395, 506, 526, 618, 730, 892
Alw21I GWGCWC 1 cut(s) 508
AlwI GGATC 1 cut(s) 403
AoxI GGCC 6 cut(s) 39, 297, 322, 415, 552, 703
ApeKI GCWGC 4 cut(s) 89, 275, 386, 889
ApoI RAATTY 3 cut(s) 126, 317, 494
AseI ATTAAT 1 cut(s) 903
Asp700I GAANNNNTTC 2 cut(s) 167, 569
AspLEI GCGC 4 cut(s) 629, 702, 738, 926
AspS9I GGNCC 1 cut(s) 814
AsuHPI GGTGA 2 cut(s) 845, 886
AvaII GGWCC 1 cut(s) 814
BaeGI GKGCMC 1 cut(s) 639
BalI TGGCCA 1 cut(s) 554
BanI GGYRCC 2 cut(s) 484, 634
BanII GRGCYC 1 cut(s) 508
Bbv12I GWGCWC 1 cut(s) 508
BbvI GCAGC 4 cut(s) 101, 262, 373, 901
BccI CCATC 4 cut(s) 29, 475, 600, 625
BceAI ACGGC 2 cut(s) 49, 402
BciVI GTATCC 1 cut(s) 554
BfaI CTAG 1 cut(s) 779
BfuI GTATCC 1 cut(s) 554
BmcAI AGTACT 1 cut(s) 221
Bme18I GGWCC 1 cut(s) 814
BmgT120I GGNCC 1 cut(s) 814
BmiI GGNNCC 3 cut(s) 486, 636, 816
BmsI GCATC 2 cut(s) 447, 784
Bsa29I ATCGAT 2 cut(s) 587, 912
BsaBI GATNNNNATC 1 cut(s) 407
BsaJI CCNNGG 4 cut(s) 93, 147, 412, 818
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 27, 811, 862
Bse118I RCCGGY 2 cut(s) 896, 926
Bse1I ACTGG 2 cut(s) 217, 789
Bse8I GATNNNNATC 1 cut(s) 407
BseCI ATCGAT 2 cut(s) 587, 912
BseDI CCNNGG 4 cut(s) 93, 147, 412, 818
BseGI GGATG 2 cut(s) 408, 611
BseJI GATNNNNATC 1 cut(s) 407
BseLI CCNNNNNNNGG 4 cut(s) 11, 27, 811, 862
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 2 cut(s) 217, 789
BseRI GAGGAG 2 cut(s) 779, 850
BseSI GKGCMC 1 cut(s) 639
BseX3I CGGCCG 2 cut(s) 322, 415
BseXI GCAGC 4 cut(s) 101, 262, 373, 901
BseYI CCCAGC 1 cut(s) 600
Bsh1236I CGCG 5 cut(s) 629, 702, 744, 800, 924
Bsh1285I CGRYCG 4 cut(s) 294, 325, 418, 946
BshFI GGCC 6 cut(s) 41, 299, 324, 417, 554, 705
BshNI GGYRCC 2 cut(s) 484, 634
BshVI ATCGAT 2 cut(s) 587, 912
BsiEI CGRYCG 4 cut(s) 294, 325, 418, 946
BsiHKAI GWGCWC 1 cut(s) 508
BsiSI CCGG 5 cut(s) 48, 325, 366, 897, 927
BslI CCNNNNNNNGG 4 cut(s) 11, 27, 811, 862
BsmI GAATGC 1 cut(s) 738
BsnI GGCC 6 cut(s) 41, 299, 324, 417, 554, 705
Bsp1286I GDGCHC 2 cut(s) 508, 639
Bsp143I GATC 7 cut(s) 291, 408, 584, 588, 909, 943, 954
Bsp19I CCATGG 2 cut(s) 93, 147
BspANI GGCC 6 cut(s) 41, 299, 324, 417, 554, 705
BspCNI CTCAG 1 cut(s) 469
BspDI ATCGAT 2 cut(s) 587, 912
BspFNI CGCG 5 cut(s) 629, 702, 744, 800, 924
BspLI GGNNCC 3 cut(s) 486, 636, 816
BspPI GGATC 1 cut(s) 403
BspT107I GGYRCC 2 cut(s) 484, 634
BsrFI RCCGGY 2 cut(s) 896, 926
BsrI ACTGG 2 cut(s) 217, 789
BssAI RCCGGY 2 cut(s) 896, 926
BssECI CCNNGG 4 cut(s) 93, 147, 412, 818
BssMI GATC 7 cut(s) 291, 408, 584, 588, 909, 943, 954
BssT1I CCWWGG 2 cut(s) 93, 147
Bst4CI ACNGT 3 cut(s) 224, 307, 333
BstC8I GCNNGC 3 cut(s) 6, 639, 707
BstDEI CTNAG 1 cut(s) 477
BstDSI CCRYGG 3 cut(s) 93, 147, 818
BstF5I GGATG 2 cut(s) 408, 611
BstFNI CGCG 5 cut(s) 629, 702, 744, 800, 924
BstHHI GCGC 4 cut(s) 629, 702, 738, 926
BstKTI GATC 7 cut(s) 294, 411, 587, 591, 912, 946, 957
BstMBI GATC 7 cut(s) 291, 408, 584, 588, 909, 943, 954
BstMCI CGRYCG 4 cut(s) 294, 325, 418, 946
BstMWI GCNNNNNNNGC 6 cut(s) 33, 392, 624, 706, 744, 794
BstNSI RCATGY 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 639
BstUI CGCG 5 cut(s) 629, 702, 744, 800, 924
BstV1I GCAGC 4 cut(s) 101, 262, 373, 901
BstX2I RGATCY 1 cut(s) 954
BstYI RGATCY 1 cut(s) 954
BstZI CGGCCG 2 cut(s) 322, 415
Bsu15I ATCGAT 2 cut(s) 587, 912
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 6 cut(s) 41, 299, 324, 417, 554, 705
BsuTUI ATCGAT 2 cut(s) 587, 912
BtgI CCRYGG 3 cut(s) 93, 147, 818
BtgZI GCGATG 1 cut(s) 644
BtsCI GGATG 2 cut(s) 408, 611
BtsIMutI CAGTG 3 cut(s) 465, 778, 796
Cac8I GCNNGC 3 cut(s) 6, 639, 707
CfoI GCGC 4 cut(s) 629, 702, 738, 926
Cfr10I RCCGGY 2 cut(s) 896, 926
Cfr13I GGNCC 1 cut(s) 814
ClaI ATCGAT 2 cut(s) 587, 912
Csp6I GTAC 3 cut(s) 220, 268, 844
CviAII CATG 8 cut(s) 78, 94, 148, 250, 337, 691, 710, 862
CviQI GTAC 3 cut(s) 220, 268, 844
DdeI CTNAG 1 cut(s) 477
DpnI GATC 7 cut(s) 293, 410, 586, 590, 911, 945, 956
DpnII GATC 7 cut(s) 291, 408, 584, 588, 909, 943, 954
EaeI YGGCCR 5 cut(s) 297, 322, 415, 552, 703
EagI CGGCCG 2 cut(s) 322, 415
Ecl136II GAGCTC 1 cut(s) 506
EclXI CGGCCG 2 cut(s) 322, 415
Eco130I CCWWGG 2 cut(s) 93, 147
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 508
Eco32I GATATC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 814
Eco52I CGGCCG 2 cut(s) 322, 415
Eco53kI GAGCTC 1 cut(s) 506
Eco57I CTGAAG 2 cut(s) 182, 716
EcoICRI GAGCTC 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 814
EcoRV GATATC 1 cut(s) 121
EcoT14I CCWWGG 2 cut(s) 93, 147
EcoT22I ATGCAT 1 cut(s) 863
EcoT38I GRGCYC 1 cut(s) 508
ErhI CCWWGG 2 cut(s) 93, 147
FaeI CATG 8 cut(s) 81, 97, 151, 253, 340, 694, 713, 865
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FatI CATG 8 cut(s) 77, 93, 147, 249, 336, 690, 709, 861
FauI CCCGC 2 cut(s) 20, 646
FokI GGATG 2 cut(s) 415, 618
FriOI GRGCYC 1 cut(s) 508
FspBI CTAG 1 cut(s) 779
GlaI GCGC 4 cut(s) 628, 701, 737, 925
GsaI CCCAGC 1 cut(s) 604
HaeIII GGCC 6 cut(s) 41, 299, 324, 417, 554, 705
HapII CCGG 5 cut(s) 48, 325, 366, 897, 927
HhaI GCGC 4 cut(s) 629, 702, 738, 926
Hin1II CATG 8 cut(s) 81, 97, 151, 253, 340, 694, 713, 865
Hin6I GCGC 4 cut(s) 627, 700, 736, 924
HinP1I GCGC 4 cut(s) 627, 700, 736, 924
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 2 cut(s) 152, 444
HpaII CCGG 5 cut(s) 48, 325, 366, 897, 927
HphI GGTGA 2 cut(s) 845, 886
Hpy166II GTNNAC 2 cut(s) 246, 255
Hpy188I TCNGA 5 cut(s) 105, 125, 443, 478, 959
Hpy188III TCNNGA 2 cut(s) 160, 400
Hpy8I GTNNAC 2 cut(s) 246, 255
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 2 cut(s) 187, 889
HpyCH4III ACNGT 3 cut(s) 224, 307, 333
HpyCH4IV ACGT 3 cut(s) 66, 143, 242
HpyCH4V TGCA 2 cut(s) 89, 861
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 392, 624, 706, 744, 794
HpyF3I CTNAG 1 cut(s) 477
HpySE526I ACGT 3 cut(s) 66, 143, 242
Hsp92II CATG 8 cut(s) 81, 97, 151, 253, 340, 694, 713, 865
HspAI GCGC 4 cut(s) 627, 700, 736, 924
Kzo9I GATC 7 cut(s) 291, 408, 584, 588, 909, 943, 954
LmnI GCTCC 2 cut(s) 368, 503
Lsp1109I GCAGC 4 cut(s) 101, 262, 373, 901
LweI GCATC 2 cut(s) 447, 784
MaeI CTAG 1 cut(s) 779
MaeII ACGT 3 cut(s) 66, 143, 242
MaeIII GTNAC 3 cut(s) 139, 307, 773
MalI GATC 7 cut(s) 293, 410, 586, 590, 911, 945, 956
MboI GATC 7 cut(s) 291, 408, 584, 588, 909, 943, 954
MboII GAAGA 1 cut(s) 175
MfeI CAATTG 1 cut(s) 537
MflI RGATCY 1 cut(s) 954
MhlI GDGCHC 2 cut(s) 508, 639
MlsI TGGCCA 1 cut(s) 554
MluCI AATT 5 cut(s) 126, 317, 494, 537, 807
MluNI TGGCCA 1 cut(s) 554
MnlI CCTC 9 cut(s) 298, 422, 482, 757, 805, 828, 831, 861, 881
Mox20I TGGCCA 1 cut(s) 554
Mph1103I ATGCAT 1 cut(s) 863
MroXI GAANNNNTTC 2 cut(s) 167, 569
MscI TGGCCA 1 cut(s) 554
MseI TTAA 1 cut(s) 903
MslI CAYNNNNRTG 1 cut(s) 905
Msp20I TGGCCA 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 5 cut(s) 48, 325, 366, 897, 927
MunI CAATTG 1 cut(s) 537
Mva1269I GAATGC 1 cut(s) 738
MvnI CGCG 5 cut(s) 629, 702, 744, 800, 924
MwoI GCNNNNNNNGC 6 cut(s) 33, 392, 624, 706, 744, 794
NcoI CCATGG 2 cut(s) 93, 147
NdeII GATC 7 cut(s) 291, 408, 584, 588, 909, 943, 954
NlaIII CATG 8 cut(s) 81, 97, 151, 253, 340, 694, 713, 865
NlaIV GGNNCC 3 cut(s) 486, 636, 816
NmeAIII GCCGAG 1 cut(s) 393
NmuCI GTSAC 2 cut(s) 307, 773
NsiI ATGCAT 1 cut(s) 863
NspI RCATGY 1 cut(s) 81
PceI AGGCCT 1 cut(s) 41
PciI ACATGT 1 cut(s) 77
PctI GAATGC 1 cut(s) 738
PdmI GAANNNNTTC 2 cut(s) 167, 569
PfeI GAWTC 2 cut(s) 152, 444
Ple19I CGATCG 2 cut(s) 294, 946
PpuMI RGGWCCY 1 cut(s) 814
PscI ACATGT 1 cut(s) 77
PshBI ATTAAT 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 508
Psp1406I AACGTT 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 814
PspFI CCCAGC 1 cut(s) 600
PspN4I GGNNCC 3 cut(s) 486, 636, 816
PspPI GGNCC 1 cut(s) 814
PspPPI RGGWCCY 1 cut(s) 814
PsrI GAACNNNNNNTAC 2 cut(s) 203, 235
PsuI RGATCY 1 cut(s) 954
PvuI CGATCG 2 cut(s) 294, 946
RsaI GTAC 3 cut(s) 221, 269, 845
RsaNI GTAC 3 cut(s) 220, 268, 844
RseI CAYNNNNRTG 1 cut(s) 905
SacI GAGCTC 1 cut(s) 508
SaqAI TTAA 1 cut(s) 903
Sau3AI GATC 7 cut(s) 291, 408, 584, 588, 909, 943, 954
Sau96I GGNCC 1 cut(s) 814
ScaI AGTACT 1 cut(s) 221
SduI GDGCHC 2 cut(s) 508, 639
SfaNI GCATC 2 cut(s) 447, 784
SinI GGWCC 1 cut(s) 814
SmiMI CAYNNNNRTG 1 cut(s) 905
Sse9I AATT 5 cut(s) 126, 317, 494, 537, 807
SseBI AGGCCT 1 cut(s) 41
SspMI CTAG 1 cut(s) 779
SstI GAGCTC 1 cut(s) 508
StuI AGGCCT 1 cut(s) 41
StyI CCWWGG 2 cut(s) 93, 147
TaaI ACNGT 3 cut(s) 224, 307, 333
TaiI ACGT 3 cut(s) 69, 146, 245
TaqI TCGA 3 cut(s) 290, 587, 912
TasI AATT 5 cut(s) 126, 317, 494, 537, 807
TatI WGTACW 3 cut(s) 219, 267, 843
TauI GCSGC 7 cut(s) 302, 386, 627, 705, 747, 788, 800
TfiI GAWTC 2 cut(s) 152, 444
Tru1I TTAA 1 cut(s) 903
Tru9I TTAA 1 cut(s) 903
TscAI CASTG 3 cut(s) 465, 778, 796
TseFI GTSAC 2 cut(s) 307, 773
TseI GCWGC 4 cut(s) 89, 275, 386, 889
Tsp45I GTSAC 2 cut(s) 307, 773
TspRI CASTG 3 cut(s) 465, 778, 796
VpaK11BI GGWCC 1 cut(s) 814
VspI ATTAAT 1 cut(s) 903
XapI RAATTY 3 cut(s) 126, 317, 494
XceI RCATGY 1 cut(s) 81
XmnI GAANNNNTTC 2 cut(s) 167, 569
XspI CTAG 1 cut(s) 779
ZrmI AGTACT 1 cut(s) 221
Zsp2I ATGCAT 1 cut(s) 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.