pycom10g25670

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007622
Physical Location & Seq
Reverse (-)
27220210 .. 27222169
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 786 bp
ATGGCCGTCGCTGACCAAAAGTCGTCGTCGTCGTCTTCGTCTTCGTCTTCGTCCGACGGAAAAGTCTGGGGCTTCTTCAAGCGACCGTTTCGGACGTCGGGGAAAGCCGCCGTTACGATGTCGTCCTCGTCGTGGCACAACGCTCCCCACCACCATAGTAACGCTCAGGGCGACGGATCGAATCAAAACGCTGCGAATTCGGTGTCGTCCGTCGCCAGGTCGCTGCTTCCGACTCGCCGTAGACTCAGGATCGATCCCAACAATAAACTCTATTTTCCCTGTGAACCCGGAAAGCAGGTTAGGAGTGCCATTAGGATTAAAAACACCAGCAAGTCCTTTGTAGCATTCAAGTTCCAAACAACTGCACCGAAAAGTTGTTTCATGCGTCCTCCAGGAGCGATTCTTAGTCCTGGCGAGAGTCTTATAGCGACTGTATTCAAGTTTGTTGAGGCCCCCGAGAAGAATGAAAAACTAGTGGATCGAAAGAGCAGAGTTAAGTTTAAAATCATGAGCTTGAAGGTGAAGGGACCAATGGAATATGTACCTGAGCTGTTTGATGAGCAAAAGGACCAAGTGGCCGTAGAGCAGATACTACGGGTTGTCTTTCTAGATCCAGAGCGTTCTACTCCTTTGGCTGATGCTGATGCTGCGCTGGAGGCACGCAAGAAACCTGCGGAAGAAACTGGTCCAAAGATTATTGGAGAAGGACTTGTCATAGATGAATGGAAGGAGAGGAGGGAAAGATATCTCGCCCGTCAGCAAGTTGAAGGCGTGGACTCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

262

Amino Acids

28.95

Weight (kDa)

9.74

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 82 - 192 1.1e-21 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AatII GACGTC 1 cut(s) 98
Acc36I ACCTGC 2 cut(s) 286, 679
AccI GTMKAC 1 cut(s) 241
AciI CCGC 2 cut(s) 108, 674
AclWI GGATC 5 cut(s) 184, 248, 257, 486, 605
AcoI YGGCCR 2 cut(s) 3, 576
AcsI RAATTY 1 cut(s) 196
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 1 cut(s) 543
AfiI CCNNNNNNNGG 2 cut(s) 132, 216
AgsI TTSAA 5 cut(s) 79, 349, 439, 517, 767
AhdI GACNNNNNGTC 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 472
AjnI CCWGG 3 cut(s) 215, 391, 409
AluBI AGCT 2 cut(s) 513, 550
AluI AGCT 2 cut(s) 513, 550
AlwI GGATC 5 cut(s) 184, 248, 257, 486, 605
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 3 cut(s) 3, 450, 576
ApeKI GCWGC 3 cut(s) 191, 223, 647
ApoI RAATTY 1 cut(s) 196
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 4 cut(s) 451, 527, 568, 686
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 532
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 3 cut(s) 527, 568, 686
BbsI GAAGAC 3 cut(s) 27, 33, 39
BbvI GCAGC 3 cut(s) 178, 210, 634
BceAI ACGGC 3 cut(s) 95, 222, 563
BciT130I CCWGG 3 cut(s) 217, 393, 411
BcnI CCSGG 1 cut(s) 288
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 2 cut(s) 473, 608
BfuAI ACCTGC 2 cut(s) 286, 679
BisI GCNGC 4 cut(s) 108, 192, 224, 648
BlsI GCNGC 4 cut(s) 109, 193, 225, 649
Bme1390I CCNGG 4 cut(s) 217, 288, 393, 411
Bme18I GGWCC 3 cut(s) 527, 568, 686
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 455
BmgT120I GGNCC 4 cut(s) 451, 527, 568, 686
BmiI GGNNCC 2 cut(s) 453, 528
BmrFI CCNGG 4 cut(s) 217, 288, 393, 411
BmsI GCATC 2 cut(s) 628, 634
BpiI GAAGAC 3 cut(s) 27, 33, 39
BpmI CTGGAG 2 cut(s) 375, 674
Bpu10I CCTNAGC 2 cut(s) 165, 546
BpuMI CCSGG 1 cut(s) 288
Bsa29I ATCGAT 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 95
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 216
Bse1I ACTGG 1 cut(s) 688
BseBI CCWGG 3 cut(s) 217, 393, 411
BseCI ATCGAT 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 132, 216
BseMII CTCAG 3 cut(s) 179, 259, 537
BseNI ACTGG 1 cut(s) 688
BseRI GAGGAG 1 cut(s) 748
BseXI GCAGC 3 cut(s) 178, 210, 634
BsgI GTGCAG 1 cut(s) 348
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 3 cut(s) 5, 452, 578
BshVI ATCGAT 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 2 cut(s) 132, 216
BsmFI GGGAC 1 cut(s) 540
BsmI GAATGC 1 cut(s) 344
BsnI GGCC 3 cut(s) 5, 452, 578
BsoBI CYCGRG 1 cut(s) 455
Bsp143I GATC 5 cut(s) 176, 249, 253, 478, 610
BspACI CCGC 2 cut(s) 108, 674
BspANI GGCC 3 cut(s) 5, 452, 578
BspCNI CTCAG 3 cut(s) 178, 258, 538
BspDI ATCGAT 1 cut(s) 252
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 2 cut(s) 453, 528
BspMI ACCTGC 2 cut(s) 286, 679
BspPI GGATC 5 cut(s) 184, 248, 257, 486, 605
BsrI ACTGG 1 cut(s) 688
BssMI GATC 5 cut(s) 176, 249, 253, 478, 610
BssNI GRCGYC 1 cut(s) 95
Bst2UI CCWGG 3 cut(s) 217, 393, 411
Bst4CI ACNGT 2 cut(s) 87, 433
BstACI GRCGYC 1 cut(s) 95
BstC8I GCNNGC 1 cut(s) 661
BstDEI CTNAG 4 cut(s) 165, 245, 404, 546
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 5 cut(s) 179, 252, 256, 481, 613
BstMBI GATC 5 cut(s) 176, 249, 253, 478, 610
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 2 cut(s) 647, 656
BstNI CCWGG 3 cut(s) 217, 393, 411
BstSCI CCNGG 4 cut(s) 215, 286, 391, 409
BstV1I GCAGC 3 cut(s) 178, 210, 634
BstV2I GAAGAC 3 cut(s) 27, 33, 39
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 3 cut(s) 5, 452, 578
BsuTUI ATCGAT 1 cut(s) 252
BveI ACCTGC 2 cut(s) 286, 679
Cac8I GCNNGC 1 cut(s) 661
CciI TCATGA 1 cut(s) 507
CfoI GCGC 1 cut(s) 652
Cfr13I GGNCC 4 cut(s) 451, 527, 568, 686
ClaI ATCGAT 1 cut(s) 252
CseI GACGC 1 cut(s) 374
Csp6I GTAC 1 cut(s) 542
CviAII CATG 3 cut(s) 382, 508, 783
CviJI RGCY 8 cut(s) 5, 72, 107, 452, 513, 550, 578, 635
CviKI_1 RGCY 8 cut(s) 5, 72, 107, 452, 513, 550, 578, 635
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 4 cut(s) 165, 245, 404, 546
DpnI GATC 5 cut(s) 178, 251, 255, 480, 612
DpnII GATC 5 cut(s) 176, 249, 253, 478, 610
DraI TTTAAA 1 cut(s) 502
DrdI GACNNNNNNGTC 1 cut(s) 62
DriI GACNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 2 cut(s) 3, 576
Eam1105I GACNNNNNGTC 1 cut(s) 19
Eco32I GATATC 1 cut(s) 746
Eco47I GGWCC 3 cut(s) 527, 568, 686
Eco88I CYCGRG 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 451
EcoRI GAATTC 1 cut(s) 196
EcoRII CCWGG 3 cut(s) 215, 391, 409
EcoRV GATATC 1 cut(s) 746
FaeI CATG 3 cut(s) 385, 511, 786
FaiI YATR 7 cut(s) 156, 383, 425, 509, 540, 716, 784
FaqI GGGAC 1 cut(s) 540
FatI CATG 3 cut(s) 381, 507, 782
FblI GTMKAC 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 108, 192, 224, 648
Fsp4HI GCNGC 4 cut(s) 108, 192, 224, 648
FspBI CTAG 2 cut(s) 473, 608
GlaI GCGC 1 cut(s) 651
GluI GCNGC 4 cut(s) 108, 192, 224, 648
GsuI CTGGAG 2 cut(s) 375, 674
HaeIII GGCC 3 cut(s) 5, 452, 578
HapII CCGG 1 cut(s) 288
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 652
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 3 cut(s) 385, 511, 786
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HinfI GANTC 6 cut(s) 181, 232, 243, 400, 418, 776
HpaII CCGG 1 cut(s) 288
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 3 cut(s) 242, 284, 775
Hpy188I TCNGA 3 cut(s) 55, 93, 231
Hpy188III TCNNGA 4 cut(s) 247, 508, 608, 614
Hpy8I GTNNAC 3 cut(s) 242, 284, 775
Hpy99I CGWCG 9 cut(s) 11, 28, 31, 34, 59, 100, 133, 176, 215
HpyAV CCTTC 5 cut(s) 511, 517, 698, 721, 761
HpyCH4III ACNGT 2 cut(s) 87, 433
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 647, 656
HpyF3I CTNAG 4 cut(s) 165, 245, 404, 546
HpySE526I ACGT 1 cut(s) 95
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 3 cut(s) 385, 511, 786
HspAI GCGC 1 cut(s) 650
Kzo9I GATC 5 cut(s) 176, 249, 253, 478, 610
LmnI GCTCC 2 cut(s) 148, 395
Lsp1109I GCAGC 3 cut(s) 178, 210, 634
LweI GCATC 2 cut(s) 628, 634
MaeI CTAG 2 cut(s) 473, 608
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 112, 158
MalI GATC 5 cut(s) 178, 251, 255, 480, 612
MboI GATC 5 cut(s) 176, 249, 253, 478, 610
MboII GAAGA 6 cut(s) 27, 33, 39, 67, 472, 689
MflI RGATCY 1 cut(s) 610
MluCI AATT 1 cut(s) 196
MlyI GAGTC 4 cut(s) 226, 237, 427, 770
MmeI TCCRAC 2 cut(s) 78, 254
MnlI CCTC 6 cut(s) 136, 399, 442, 649, 726, 729
MseI TTAA 3 cut(s) 318, 495, 501
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 4 cut(s) 217, 288, 393, 411
Mva1269I GAATGC 1 cut(s) 344
MvaI CCWGG 3 cut(s) 217, 393, 411
MwoI GCNNNNNNNGC 2 cut(s) 647, 656
NciI CCSGG 1 cut(s) 288
NdeII GATC 5 cut(s) 176, 249, 253, 478, 610
NlaIII CATG 3 cut(s) 385, 511, 786
NlaIV GGNNCC 2 cut(s) 453, 528
PagI TCATGA 1 cut(s) 507
PcsI WCGNNNNNNNCGW 5 cut(s) 29, 35, 128, 168, 227
PctI GAATGC 1 cut(s) 344
PfeI GAWTC 2 cut(s) 181, 400
PfoI TCCNGGA 1 cut(s) 391
PkrI GCNGC 4 cut(s) 109, 193, 225, 649
PleI GAGTC 4 cut(s) 226, 237, 426, 770
PpsI GAGTC 4 cut(s) 226, 237, 426, 770
Psp6I CCWGG 3 cut(s) 215, 391, 409
PspGI CCWGG 3 cut(s) 215, 391, 409
PspN4I GGNNCC 2 cut(s) 453, 528
PspPI GGNCC 4 cut(s) 451, 527, 568, 686
PsuI RGATCY 1 cut(s) 610
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
SaqAI TTAA 3 cut(s) 318, 495, 501
SatI GCNGC 4 cut(s) 108, 192, 224, 648
Sau3AI GATC 5 cut(s) 176, 249, 253, 478, 610
Sau96I GGNCC 4 cut(s) 451, 527, 568, 686
SchI GAGTC 4 cut(s) 226, 237, 427, 770
ScrFI CCNGG 4 cut(s) 217, 288, 393, 411
SetI ASST 8 cut(s) 98, 221, 300, 515, 522, 547, 552, 673
SfaNI GCATC 2 cut(s) 628, 634
SinI GGWCC 3 cut(s) 527, 568, 686
SpeI ACTAGT 1 cut(s) 472
Sse9I AATT 1 cut(s) 196
SsiI CCGC 2 cut(s) 108, 674
SspMI CTAG 2 cut(s) 473, 608
StyD4I CCNGG 4 cut(s) 215, 286, 391, 409
TaaI ACNGT 2 cut(s) 87, 433
TaiI ACGT 1 cut(s) 98
TaqI TCGA 3 cut(s) 179, 252, 481
TasI AATT 1 cut(s) 196
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 181, 400
Tru1I TTAA 3 cut(s) 318, 495, 501
Tru9I TTAA 3 cut(s) 318, 495, 501
TseI GCWGC 3 cut(s) 191, 223, 647
TspDTI ATGAA 3 cut(s) 370, 480, 735
TspGWI ACGGA 3 cut(s) 72, 189, 199
VpaK11BI GGWCC 3 cut(s) 527, 568, 686
XapI RAATTY 1 cut(s) 196
XbaI TCTAGA 1 cut(s) 607
XmiI GTMKAC 1 cut(s) 241
XspI CTAG 2 cut(s) 473, 608
ZraI GACGTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.