pycom10g28170

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008511
Physical Location & Seq
Forward (+)
28862994 .. 28865964
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 954 bp
ATGATTGGCTTGGGCCTTTTACTCTGTCTTCATTGGGACACGGATATCCCCTCTGCGTTTGGTCTTTTCACATTCGCATTCCTGGACCGACTGAAAGACAAACACCGTGTAGTGAAAATCCAATCGATAAGGAGGCGAGGCAGAGCAGAACAGAGAGAGATATACCCAAAGCGCAACCAAAGACTGTCGTTTAGCTCTGATTCTTTGACTGCCCGCGAAGCTCCAACAATGGCGGCAGATCTGGTACTTGACACAGCCATCAGAGACTGGGTTCTCATCCCTCTCTCGGTGGTCATGGTTCTCATCGGCGTCCTCCGATACTTCGTCTCCAAGCTCATGCGATCCAACCAAGTCCCCGATGCCAAAATTGTCAAAGAAGGGGCTCGGAACTTACGTGCTGCTGCTAATTTCATACCTCCAAAGTCGTTTCGTGCTCGCCGATTGTATTACAACAACGAGGAAAATGGGTTGTTAATTGTTCCTAAGGGTCAGGCACAGAATCCACAAGCGCAAATGTTCTCTGATCCCAACATGGCCATGGATATGATGAAGAAAAATCTGTCCATGATAATACCCCAGACTCTTACTTTTGCATGGGTCAACTTTTTCTTCTCTGGATTTGTAGCAGCCAAAATTCCCTTTCCTCTTACTCAAAGGTTCAGGTCTATGCTTCAGAATGGGATTGACTTGAGCACTGTTGATGTTAGCTACGTCAGTAGCCGCTCATGGTACTTTCTTAATCTGTTTGGATTAAGGGGTTTGTTTAGTCTCATTCTGGGAGAAGAAAATGCCATGGACGATACTCAGAAAATGATGCAAATGAGTGGCTTTGGATTTGATCCATCAAAGAGCTTGGGAGCTGAGAAGGACAGTCTTGATATAGTCCAGCATGACTGGGCCTTACCAAAATTTGAGCATCGCGCCGAAGCAGTATTGAGAGAACTTGCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF106

Protein Analysis

318

Amino Acids

36.18

Weight (kDa)

9.76

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC3_TMCO1 PF01956 80 - 263 3.1e-49 Integral membrane protein EMC3/TMCO1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 723
AccII CGCG 2 cut(s) 216, 921
AciI CCGC 3 cut(s) 214, 233, 721
AclWI GGATC 3 cut(s) 336, 518, 833
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 2 cut(s) 633, 908
AcuI CTGAAG 1 cut(s) 656
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 2 cut(s) 246, 731
AfiI CCNNNNNNNGG 1 cut(s) 286
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 6 cut(s) 195, 221, 334, 708, 852, 860
AluI AGCT 6 cut(s) 195, 221, 334, 708, 852, 860
Alw21I GWGCWC 2 cut(s) 436, 695
Alw26I GTCTC 3 cut(s) 258, 331, 773
AlwI GGATC 3 cut(s) 336, 518, 833
AlwNI CAGNNNCTG 1 cut(s) 267
AoxI GGCC 3 cut(s) 13, 534, 897
ApeKI GCWGC 3 cut(s) 398, 401, 626
ApoI RAATTY 2 cut(s) 633, 908
AspLEI GCGC 3 cut(s) 174, 511, 923
AspS9I GGNCC 3 cut(s) 13, 85, 897
AvaII GGWCC 1 cut(s) 85
AxyI CCTNAGG 1 cut(s) 483
BalI TGGCCA 1 cut(s) 536
BanII GRGCYC 1 cut(s) 385
BbsI GAAGAC 1 cut(s) 20
Bbv12I GWGCWC 2 cut(s) 436, 695
BbvI GCAGC 3 cut(s) 385, 388, 638
BccI CCATC 2 cut(s) 266, 850
BciT130I CCWGG 1 cut(s) 83
BcoDI GTCTC 3 cut(s) 258, 331, 773
BglII AGATCT 1 cut(s) 238
BisI GCNGC 5 cut(s) 234, 399, 402, 627, 721
BlsI GCNGC 5 cut(s) 235, 400, 403, 628, 722
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 85
BmgT120I GGNCC 3 cut(s) 13, 85, 897
BmrFI CCNGG 1 cut(s) 83
BmrI ACTGGG 2 cut(s) 277, 904
BmsI GCATC 3 cut(s) 349, 804, 925
BmuI ACTGGG 2 cut(s) 277, 904
BpiI GAAGAC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 709
Bsa29I ATCGAT 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 537, 792
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse1I ACTGG 2 cut(s) 272, 899
Bse21I CCTNAGG 1 cut(s) 483
BseBI CCWGG 1 cut(s) 83
BseCI ATCGAT 1 cut(s) 125
BseDI CCNNGG 2 cut(s) 537, 792
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 286
BseMII CTCAG 2 cut(s) 818, 852
BseNI ACTGG 2 cut(s) 272, 899
BseXI GCAGC 3 cut(s) 385, 388, 638
Bsh1236I CGCG 2 cut(s) 216, 921
BshFI GGCC 3 cut(s) 15, 536, 899
BshVI ATCGAT 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 436, 695
BslFI GGGAC 2 cut(s) 50, 338
BslI CCNNNNNNNGG 1 cut(s) 286
BsmAI GTCTC 3 cut(s) 258, 331, 773
BsmBI CGTCTC 1 cut(s) 331
BsmFI GGGAC 2 cut(s) 50, 338
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 3 cut(s) 15, 536, 899
Bsp1286I GDGCHC 3 cut(s) 385, 436, 695
Bsp143I GATC 4 cut(s) 238, 341, 523, 838
Bsp19I CCATGG 2 cut(s) 537, 792
BspACI CCGC 3 cut(s) 214, 233, 721
BspANI GGCC 3 cut(s) 15, 536, 899
BspCNI CTCAG 2 cut(s) 817, 853
BspDI ATCGAT 1 cut(s) 125
BspFNI CGCG 2 cut(s) 216, 921
BspPI GGATC 3 cut(s) 336, 518, 833
BsrBI CCGCTC 1 cut(s) 723
BsrI ACTGG 2 cut(s) 272, 899
BssECI CCNNGG 2 cut(s) 537, 792
BssMI GATC 4 cut(s) 238, 341, 523, 838
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 2 cut(s) 537, 792
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 4 cut(s) 107, 186, 697, 872
BstACI GRCGYC 1 cut(s) 309
BstBAI YACGTR 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 214, 436
BstDEI CTNAG 3 cut(s) 483, 804, 861
BstDSI CCRYGG 2 cut(s) 537, 792
BstF5I GGATG 1 cut(s) 276
BstFNI CGCG 2 cut(s) 216, 921
BstHHI GCGC 3 cut(s) 174, 511, 923
BstKTI GATC 4 cut(s) 241, 344, 526, 841
BstMAI GTCTC 3 cut(s) 258, 331, 773
BstMBI GATC 4 cut(s) 238, 341, 523, 838
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstUI CGCG 2 cut(s) 216, 921
BstV1I GCAGC 3 cut(s) 385, 388, 638
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
Bsu15I ATCGAT 1 cut(s) 125
Bsu36I CCTNAGG 1 cut(s) 483
BsuRI GGCC 3 cut(s) 15, 536, 899
BsuTUI ATCGAT 1 cut(s) 125
BtgI CCRYGG 2 cut(s) 537, 792
BtgZI GCGATG 1 cut(s) 902
BtsCI GGATG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 693
Cac8I GCNNGC 2 cut(s) 214, 436
CaiI CAGNNNCTG 1 cut(s) 267
CfoI GCGC 3 cut(s) 174, 511, 923
Cfr13I GGNCC 3 cut(s) 13, 85, 897
ClaI ATCGAT 1 cut(s) 125
CseI GACGC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 245, 730
CviAII CATG 9 cut(s) 295, 337, 532, 538, 565, 594, 726, 793, 890
CviQI GTAC 2 cut(s) 245, 730
DdeI CTNAG 3 cut(s) 483, 804, 861
DpnI GATC 4 cut(s) 240, 343, 525, 840
DpnII GATC 4 cut(s) 238, 341, 523, 838
EaeI YGGCCR 1 cut(s) 534
Eco130I CCWWGG 2 cut(s) 537, 792
Eco24I GRGCYC 1 cut(s) 385
Eco32I GATATC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 656
Eco81I CCTNAGG 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 81
EcoRV GATATC 1 cut(s) 46
EcoT14I CCWWGG 2 cut(s) 537, 792
EcoT38I GRGCYC 1 cut(s) 385
ErhI CCWWGG 2 cut(s) 537, 792
Esp3I CGTCTC 1 cut(s) 331
FaeI CATG 9 cut(s) 298, 340, 535, 541, 568, 597, 729, 796, 893
FaqI GGGAC 2 cut(s) 50, 338
FatI CATG 9 cut(s) 294, 336, 531, 537, 564, 593, 725, 792, 889
FauI CCCGC 1 cut(s) 221
Fnu4HI GCNGC 5 cut(s) 234, 399, 402, 627, 721
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 5 cut(s) 234, 399, 402, 627, 721
GlaI GCGC 3 cut(s) 173, 510, 922
GluI GCNGC 5 cut(s) 234, 399, 402, 627, 721
HaeIII GGCC 3 cut(s) 15, 536, 899
HgaI GACGC 1 cut(s) 298
HhaI GCGC 3 cut(s) 174, 511, 923
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 9 cut(s) 298, 340, 535, 541, 568, 597, 729, 796, 893
Hin6I GCGC 3 cut(s) 172, 509, 921
HinP1I GCGC 3 cut(s) 172, 509, 921
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 3 cut(s) 200, 499, 580
Hpy166II GTNNAC 1 cut(s) 601
Hpy188I TCNGA 7 cut(s) 199, 263, 317, 387, 523, 675, 807
Hpy188III TCNNGA 2 cut(s) 615, 875
Hpy8I GTNNAC 1 cut(s) 601
HpyAV CCTTC 2 cut(s) 371, 859
HpyCH4III ACNGT 4 cut(s) 107, 186, 697, 872
HpyCH4IV ACGT 2 cut(s) 394, 711
HpyCH4V TGCA 2 cut(s) 593, 817
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 3 cut(s) 483, 804, 861
HpySE526I ACGT 2 cut(s) 394, 711
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 9 cut(s) 298, 340, 535, 541, 568, 597, 729, 796, 893
HspAI GCGC 3 cut(s) 172, 509, 921
Kzo9I GATC 4 cut(s) 238, 341, 523, 838
LmnI GCTCC 2 cut(s) 226, 857
Lsp1109I GCAGC 3 cut(s) 385, 388, 638
LweI GCATC 3 cut(s) 349, 804, 925
MaeII ACGT 2 cut(s) 394, 711
MalI GATC 4 cut(s) 240, 343, 525, 840
MbiI CCGCTC 1 cut(s) 723
MboI GATC 4 cut(s) 238, 341, 523, 838
MboII GAAGA 4 cut(s) 20, 562, 601, 794
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 3 cut(s) 385, 436, 695
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 5 cut(s) 366, 406, 474, 633, 908
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 2 cut(s) 248, 369
MnlI CCTC 8 cut(s) 61, 126, 131, 291, 323, 426, 451, 654
Mox20I TGGCCA 1 cut(s) 536
MscI TGGCCA 1 cut(s) 536
MseI TTAA 3 cut(s) 473, 738, 752
MslI CAYNNNNRTG 2 cut(s) 536, 542
Msp20I TGGCCA 1 cut(s) 536
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 77
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 2 cut(s) 216, 921
MwoI GCNNNNNNNGC 1 cut(s) 218
NcoI CCATGG 2 cut(s) 537, 792
NdeII GATC 4 cut(s) 238, 341, 523, 838
NlaIII CATG 9 cut(s) 298, 340, 535, 541, 568, 597, 729, 796, 893
PcsI WCGNNNNNNNCGW 2 cut(s) 391, 436
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 2 cut(s) 200, 499
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 5 cut(s) 235, 400, 403, 628, 722
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
Ppu21I YACGTR 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspPI GGNCC 3 cut(s) 13, 85, 897
PstNI CAGNNNCTG 1 cut(s) 267
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 2 cut(s) 246, 731
RsaNI GTAC 2 cut(s) 245, 730
RseI CAYNNNNRTG 2 cut(s) 536, 542
SaqAI TTAA 3 cut(s) 473, 738, 752
SatI GCNGC 5 cut(s) 234, 399, 402, 627, 721
Sau3AI GATC 4 cut(s) 238, 341, 523, 838
Sau96I GGNCC 3 cut(s) 13, 85, 897
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 3 cut(s) 385, 436, 695
SfaNI GCATC 3 cut(s) 349, 804, 925
SinI GGWCC 1 cut(s) 85
SmiMI CAYNNNNRTG 2 cut(s) 536, 542
SmlI CTYRAG 1 cut(s) 688
SmoI CTYRAG 1 cut(s) 688
Sse9I AATT 5 cut(s) 366, 406, 474, 633, 908
SsiI CCGC 3 cut(s) 214, 233, 721
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 2 cut(s) 537, 792
TaaI ACNGT 4 cut(s) 107, 186, 697, 872
TaiI ACGT 2 cut(s) 397, 714
TaqI TCGA 1 cut(s) 125
TaqII GACCGA 1 cut(s) 102
TasI AATT 5 cut(s) 366, 406, 474, 633, 908
TauI GCSGC 2 cut(s) 236, 723
TfiI GAWTC 2 cut(s) 200, 499
Tru1I TTAA 3 cut(s) 473, 738, 752
Tru9I TTAA 3 cut(s) 473, 738, 752
TscAI CASTG 1 cut(s) 700
TseI GCWGC 3 cut(s) 398, 401, 626
TspDTI ATGAA 3 cut(s) 20, 400, 563
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 1 cut(s) 700
VpaK11BI GGWCC 1 cut(s) 85
XapI RAATTY 2 cut(s) 633, 908
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.