pycom111g03510

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0010206
Physical Location & Seq
Reverse (-)
2149053 .. 2150552
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03510.1

Sequence Viewer

Length: 1500 bp
ATGAGCCAACTCCTCTATACTACACAGGCATGGAGCCGCTGCATGTATTTAGCACAACGCTGCACCAGCATGCTCAATCTCCGAGCCACCCACGCCGTCTTCGTCACCTCCGGCGTCCACCTCAACACCTACGCCGTCAGCAAACTCATCGCCTTCTGCGCATTATCGGACTCCGGCAACCTCCCGTACGCGTCTCTTCTCTTCGACCAAACCCAGACCCCCAATTTGTACATATACAACACTCTCATTAGAGCATATTATCGTAGTTCTGAGCCGCATTTGGCGATGTATTACTTCCATTTGATGTTAAAACAGAGCGGCTTAGAACCCGATAACTATACGTTTAATTTTGTGATACTGGGTTGCGTGAATTGTTCTTGTCTAGTTTCGGGGAAGGAAATTCACAGTTGGGTGGTGAAAAATGGGTTGGTTTTGTTGGATGCTCATATTCAGACGGCGCTTGTTAGATTGTACGCTGAGTGCAAGGTTTTAGATGATGCCCGGAAGCTGTTTGATGAAATTCATCAGAGAGATGTTATACAGTGGAATGTTCTTGTGAATGGGTATTTGAAATGTGGGTTGGCTTCCGAAGCTCTGGAGGTTTTCCGGGAGATGTTGGTATGTGGGTTTGAGCCTGATCACTTTTGTGTTGCTACAGGGCTCGCGGCTTGTGCTAATCTGGGGGCTCTTTGGCAAGGGAAGTGGATTCATGAGTACGTTAAAAAGAAGAAAGAGTTGAAGTCAGACGTGTTTGTTGGGACTGCGCTCGTAGATATGTATGCCAAGTGTGGTTGTATTAATTTGGCCGTGGAAGCCTTTGAGAGAATGTCGAAAAGAAATGCAGTCTCGTGGGCAGCCATGATTGGAGGGTTTGCAGCACACGGATATGCAACTGAAGTGTTTCATTGCTTGGATAGAATGCAGGTAGTGGATGGCCTCAGGCCTGATGGGGTGGTCCTTCTTGGAGTGCTAATGGCTTGTACTCATGCAGGTCTCCTCGAGAAGGGCAAGTCTCTGCTCGAAAACATGAAAACTCAATATGGGATTGTGCCAAAACATGAGCATTATAGCTGTGTTATAGACTTGCTCTGCAAGGCAGGTTGGCTAGAAGAAGCACTTCAGCTTATTAGAAGAATGCCCATGAAGCCACTTGCTTCTGTCTGGGGTGCTTTACTGAGTGGGTGCCGAGTCCATAAGAATGTTGATCTTGCAGAGCTTGCTGTTGAAGAACTTCTACAGCTAGAAAATAAGGACAGCGAAGAAGAAGCTGTTGCTTATGTTCAGTTATCAAACATTTATTTGGGTGCACGGAGAAGTGAAGATGCGGTTAGGATAAGGAAGATGATCGGTGAACGAGGAACGAATAAGACACCAGGATGCAGTATGATAGAGGTGGATGGAAAAGTTAATGAGTTTGTTTCAGGAGATGTATCACATTTACACCGAGCACAGATGGGTGCTATGCTGGAGCTGATATCTGCTGACTTAATTCAGGATTGA

Protein Analysis

500

Amino Acids

55.74

Weight (kDa)

6.39

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 74 - 124 2.4e-09 PPR repeat family
PPR_3 PF13812 74 - 123 3.7e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 178 - 224 9.2e-08 PPR repeat family
PPR PF01535 182 - 210 4e-07 PPR repeat
PPR_1 PF12854 348 - 379 2e-06 PPR repeat
E_motif PF20431 398 - 464 1.4e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 160
Acc36I ACCTGC 3 cut(s) 913, 980, 1088
AccB1I GGYRCC 1 cut(s) 1182
AccBSI CCGCTC 1 cut(s) 318
AccII CGCG 2 cut(s) 191, 665
AciI CCGC 5 cut(s) 37, 275, 318, 665, 1325
AcoI YGGCCR 1 cut(s) 804
AcsI RAATTY 2 cut(s) 399, 519
AcuI CTGAAG 2 cut(s) 915, 1103
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 5 cut(s) 188, 230, 473, 716, 982
AfiI CCNNNNNNNGG 1 cut(s) 1003
AflIII ACRYGT 2 cut(s) 189, 747
AgsI TTSAA 3 cut(s) 571, 739, 1226
AjiI CACGTC 1 cut(s) 748
AjnI CCWGG 1 cut(s) 1372
AjuI GAANNNNNNNTTGG 4 cut(s) 410, 442, 563, 595
AleI CACNNNNGTG 1 cut(s) 645
AluBI AGCT 8 cut(s) 508, 593, 1071, 1123, 1216, 1240, 1268, 1471
AluI AGCT 8 cut(s) 508, 593, 1071, 1123, 1216, 1240, 1268, 1471
Alw21I GWGCWC 2 cut(s) 1309, 1450
Alw26I GTCTC 4 cut(s) 198, 850, 998, 1017
Alw44I GTGCAC 1 cut(s) 1305
Ama87I CYCGRG 1 cut(s) 998
AoxI GGCC 3 cut(s) 804, 934, 941
ApaLI GTGCAC 1 cut(s) 1305
ApeKI GCWGC 4 cut(s) 39, 60, 854, 875
ApoI RAATTY 2 cut(s) 399, 519
ArsI GACNNNNNNTTYG 2 cut(s) 208, 240
AseI ATTAAT 1 cut(s) 798
Asp700I GAANNNNTTC 3 cut(s) 900, 1116, 1230
AspLEI GCGC 3 cut(s) 161, 460, 766
AspS9I GGNCC 1 cut(s) 955
AsuC2I CCSGG 2 cut(s) 502, 608
AsuHPI GGTGA 3 cut(s) 97, 427, 1361
AvaI CYCGRG 1 cut(s) 998
AvaII GGWCC 1 cut(s) 955
AxyI CCTNAGG 1 cut(s) 938
BaeGI GKGCMC 1 cut(s) 1309
BanI GGYRCC 1 cut(s) 1182
BanII GRGCYC 2 cut(s) 663, 688
BarI GAAGNNNNNNTAC 2 cut(s) 1218, 1250
BauI CACGAG 1 cut(s) 847
BbsI GAAGAC 1 cut(s) 91
Bbv12I GWGCWC 2 cut(s) 1309, 1450
BbvI GCAGC 4 cut(s) 26, 47, 866, 887
BccI CCATC 4 cut(s) 926, 941, 1391, 1447
BceAI ACGGC 4 cut(s) 80, 119, 471, 791
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 1374
BclI TGATCA 1 cut(s) 637
BcnI CCSGG 2 cut(s) 502, 608
BcoDI GTCTC 4 cut(s) 198, 850, 998, 1017
BfaI CTAG 3 cut(s) 383, 1106, 1241
BfmI CTRYAG 2 cut(s) 654, 1235
BfoI RGCGCY 1 cut(s) 461
BfuAI ACCTGC 3 cut(s) 913, 980, 1088
BisI GCNGC 8 cut(s) 37, 40, 61, 275, 319, 666, 855, 876
BlsI GCNGC 8 cut(s) 38, 41, 62, 276, 320, 667, 856, 877
Bme1390I CCNGG 3 cut(s) 502, 608, 1374
Bme18I GGWCC 1 cut(s) 955
BmeT110I CYCGRG 1 cut(s) 998
BmgBI CACGTC 1 cut(s) 748
BmgT120I GGNCC 1 cut(s) 955
BmiI GGNNCC 2 cut(s) 35, 1184
BmrFI CCNGG 3 cut(s) 502, 608, 1374
BmrI ACTGGG 1 cut(s) 368
BmsI GCATC 4 cut(s) 430, 487, 1312, 1367
BmuI ACTGGG 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 91
BpmI CTGGAG 2 cut(s) 617, 1487
BpuMI CCSGG 2 cut(s) 502, 608
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 998
BsaJI CCNNGG 1 cut(s) 807
Bsc4I CCNNNNNNNGG 1 cut(s) 1003
Bse1I ACTGG 1 cut(s) 363
Bse21I CCTNAGG 1 cut(s) 938
Bse3DI GCAATG 1 cut(s) 904
BseBI CCWGG 1 cut(s) 1374
BseDI CCNNGG 1 cut(s) 807
BseGI GGATG 4 cut(s) 445, 937, 1382, 1402
BseLI CCNNNNNNNGG 1 cut(s) 1003
BseMI GCAATG 1 cut(s) 904
BseMII CTCAG 4 cut(s) 261, 468, 952, 1166
BseNI ACTGG 1 cut(s) 363
BseRI GAGGAG 1 cut(s) 986
BseSI GKGCMC 1 cut(s) 1309
BseXI GCAGC 4 cut(s) 26, 47, 866, 887
BsgI GTGCAG 1 cut(s) 46
Bsh1236I CGCG 2 cut(s) 191, 665
BshFI GGCC 3 cut(s) 806, 936, 943
BshNI GGYRCC 1 cut(s) 1182
BsiHKAI GWGCWC 2 cut(s) 1309, 1450
BsiHKCI CYCGRG 1 cut(s) 998
BsiSI CCGG 4 cut(s) 111, 174, 502, 607
BsiWI CGTACG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 772
BslI CCNNNNNNNGG 1 cut(s) 1003
BsmAI GTCTC 4 cut(s) 198, 850, 998, 1017
BsmBI CGTCTC 1 cut(s) 198
BsmFI GGGAC 1 cut(s) 772
BsmI GAATGC 2 cut(s) 924, 1140
BsnI GGCC 3 cut(s) 806, 936, 943
Bso31I GGTCTC 1 cut(s) 998
BsoBI CYCGRG 1 cut(s) 998
Bsp1286I GDGCHC 4 cut(s) 663, 688, 1309, 1450
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 3 cut(s) 637, 1204, 1344
BspACI CCGC 5 cut(s) 37, 275, 318, 665, 1325
BspANI GGCC 3 cut(s) 806, 936, 943
BspCNI CTCAG 4 cut(s) 262, 469, 951, 1167
BspFNI CGCG 2 cut(s) 191, 665
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 35, 1184
BspMI ACCTGC 3 cut(s) 913, 980, 1088
BspT107I GGYRCC 1 cut(s) 1182
BspTNI GGTCTC 1 cut(s) 998
BsrBI CCGCTC 1 cut(s) 318
BsrDI GCAATG 1 cut(s) 904
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 1 cut(s) 363
BssECI CCNNGG 1 cut(s) 807
BssMI GATC 3 cut(s) 637, 1204, 1344
BssNI GRCGYC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 847
Bst2BI CACGAG 1 cut(s) 847
Bst2UI CCWGG 1 cut(s) 1374
Bst4CI ACNGT 2 cut(s) 407, 543
Bst6I CTCTTC 2 cut(s) 201, 206
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstAUI TGTACA 1 cut(s) 228
BstC8I GCNNGC 3 cut(s) 71, 663, 1218
BstDEI CTNAG 5 cut(s) 270, 322, 477, 938, 1175
BstDSI CCRYGG 1 cut(s) 807
BstENI CCTNNNNNAGG 1 cut(s) 1001
BstF5I GGATG 4 cut(s) 445, 937, 1382, 1402
BstFNI CGCG 2 cut(s) 191, 665
BstH2I RGCGCY 1 cut(s) 461
BstHHI GCGC 3 cut(s) 161, 460, 766
BstKTI GATC 3 cut(s) 640, 1207, 1347
BstMAI GTCTC 4 cut(s) 198, 850, 998, 1017
BstMBI GATC 3 cut(s) 637, 1204, 1344
BstMWI GCNNNNNNNGC 8 cut(s) 66, 92, 158, 590, 671, 812, 1144, 1217
BstNI CCWGG 1 cut(s) 1374
BstNSI RCATGY 2 cut(s) 46, 73
BstSCI CCNGG 3 cut(s) 500, 606, 1372
BstSFI CTRYAG 2 cut(s) 654, 1235
BstSLI GKGCMC 1 cut(s) 1309
BstUI CGCG 2 cut(s) 191, 665
BstV1I GCAGC 4 cut(s) 26, 47, 866, 887
BstV2I GAAGAC 1 cut(s) 91
Bsu36I CCTNAGG 1 cut(s) 938
BsuRI GGCC 3 cut(s) 806, 936, 943
BtgI CCRYGG 1 cut(s) 807
BtgZI GCGATG 2 cut(s) 133, 299
BtrI CACGTC 1 cut(s) 748
BtsCI GGATG 4 cut(s) 445, 937, 1382, 1402
BtsIMutI CAGTG 1 cut(s) 548
BveI ACCTGC 3 cut(s) 913, 980, 1088
Cac8I GCNNGC 3 cut(s) 71, 663, 1218
CciI TCATGA 1 cut(s) 709
CfoI GCGC 3 cut(s) 161, 460, 766
Cfr13I GGNCC 1 cut(s) 955
CseI GACGC 2 cut(s) 103, 180
Csp6I GTAC 5 cut(s) 187, 229, 472, 715, 981
CspCI CAANNNNNGTGG 2 cut(s) 683, 718
CviAII CATG 9 cut(s) 30, 43, 70, 710, 859, 986, 1027, 1058, 1141
CviQI GTAC 5 cut(s) 187, 229, 472, 715, 981
DdeI CTNAG 5 cut(s) 270, 322, 477, 938, 1175
DpnI GATC 3 cut(s) 639, 1206, 1346
DpnII GATC 3 cut(s) 637, 1204, 1344
EaeI YGGCCR 1 cut(s) 804
Eam1104I CTCTTC 2 cut(s) 201, 206
EarI CTCTTC 2 cut(s) 201, 206
Eco147I AGGCCT 1 cut(s) 943
Eco24I GRGCYC 2 cut(s) 663, 688
Eco31I GGTCTC 1 cut(s) 998
Eco32I GATATC 1 cut(s) 1476
Eco47I GGWCC 1 cut(s) 955
Eco57I CTGAAG 2 cut(s) 915, 1103
Eco81I CCTNAGG 1 cut(s) 938
Eco88I CYCGRG 1 cut(s) 998
EcoNI CCTNNNNNAGG 1 cut(s) 1001
EcoRII CCWGG 1 cut(s) 1372
EcoRV GATATC 1 cut(s) 1476
EcoT38I GRGCYC 2 cut(s) 663, 688
Esp3I CGTCTC 1 cut(s) 198
FaeI CATG 9 cut(s) 33, 46, 73, 713, 862, 989, 1030, 1061, 1144
FalI AAGNNNNNCTT 2 cut(s) 1101, 1133
FaqI GGGAC 1 cut(s) 772
FatI CATG 9 cut(s) 29, 42, 69, 709, 858, 985, 1026, 1057, 1140
FbaI TGATCA 1 cut(s) 637
Fnu4HI GCNGC 8 cut(s) 37, 40, 61, 275, 319, 666, 855, 876
FokI GGATG 4 cut(s) 452, 944, 1389, 1409
FriOI GRGCYC 2 cut(s) 663, 688
Fsp4HI GCNGC 8 cut(s) 37, 40, 61, 275, 319, 666, 855, 876
FspBI CTAG 3 cut(s) 383, 1106, 1241
FspI TGCGCA 1 cut(s) 160
GlaI GCGC 3 cut(s) 160, 459, 765
GluI GCNGC 8 cut(s) 37, 40, 61, 275, 319, 666, 855, 876
GsuI CTGGAG 2 cut(s) 617, 1487
HaeII RGCGCY 1 cut(s) 461
HaeIII GGCC 3 cut(s) 806, 936, 943
HapII CCGG 4 cut(s) 111, 174, 502, 607
HgaI GACGC 2 cut(s) 103, 180
HhaI GCGC 3 cut(s) 161, 460, 766
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 9 cut(s) 33, 46, 73, 713, 862, 989, 1030, 1061, 1144
Hin6I GCGC 3 cut(s) 159, 458, 764
HinP1I GCGC 3 cut(s) 159, 458, 764
HinfI GANTC 3 cut(s) 170, 706, 1188
HpaII CCGG 4 cut(s) 111, 174, 502, 607
HphI GGTGA 3 cut(s) 97, 427, 1361
Hpy166II GTNNAC 3 cut(s) 118, 1307, 1352
Hpy188I TCNGA 7 cut(s) 83, 169, 271, 453, 528, 589, 745
Hpy188III TCNNGA 5 cut(s) 596, 710, 1000, 1422, 1493
Hpy8I GTNNAC 3 cut(s) 118, 1307, 1352
HpyAV CCTTC 4 cut(s) 163, 388, 968, 997
HpyCH4III ACNGT 2 cut(s) 407, 543
HpyCH4IV ACGT 3 cut(s) 341, 717, 747
HpyF10VI GCNNNNNNNGC 8 cut(s) 66, 92, 158, 590, 671, 812, 1144, 1217
HpyF3I CTNAG 5 cut(s) 270, 322, 477, 938, 1175
HpySE526I ACGT 3 cut(s) 341, 717, 747
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 9 cut(s) 33, 46, 73, 713, 862, 989, 1030, 1061, 1144
HspAI GCGC 3 cut(s) 159, 458, 764
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 3 cut(s) 637, 1204, 1344
LmnI GCTCC 2 cut(s) 33, 1468
Lsp1109I GCAGC 4 cut(s) 26, 47, 866, 887
LweI GCATC 4 cut(s) 430, 487, 1312, 1367
MaeI CTAG 3 cut(s) 383, 1106, 1241
MaeII ACGT 3 cut(s) 341, 717, 747
MaeIII GTNAC 1 cut(s) 103
MalI GATC 3 cut(s) 639, 1206, 1346
MbiI CCGCTC 1 cut(s) 318
MboI GATC 3 cut(s) 637, 1204, 1344
MhlI GDGCHC 4 cut(s) 663, 688, 1309, 1450
MluCI AATT 7 cut(s) 223, 346, 370, 399, 519, 799, 1488
MluI ACGCGT 1 cut(s) 189
MlyI GAGTC 2 cut(s) 164, 1197
MmeI TCCRAC 1 cut(s) 417
MroXI GAANNNNTTC 3 cut(s) 900, 1116, 1230
MseI TTAA 6 cut(s) 308, 345, 720, 798, 1407, 1487
MslI CAYNNNNRTG 6 cut(s) 28, 68, 645, 885, 1197, 1375
MspA1I CMGCKG 1 cut(s) 39
MspI CCGG 4 cut(s) 111, 174, 502, 607
MspR9I CCNGG 3 cut(s) 502, 608, 1374
Mva1269I GAATGC 2 cut(s) 924, 1140
MvaI CCWGG 1 cut(s) 1374
MvnI CGCG 2 cut(s) 191, 665
MwoI GCNNNNNNNGC 8 cut(s) 66, 92, 158, 590, 671, 812, 1144, 1217
NciI CCSGG 2 cut(s) 502, 608
NdeII GATC 3 cut(s) 637, 1204, 1344
NlaIII CATG 9 cut(s) 33, 46, 73, 713, 862, 989, 1030, 1061, 1144
NlaIV GGNNCC 2 cut(s) 35, 1184
NmeAIII GCCGAG 1 cut(s) 1211
NmuCI GTSAC 1 cut(s) 103
NsbI TGCGCA 1 cut(s) 160
NspI RCATGY 2 cut(s) 46, 73
OliI CACNNNNGTG 1 cut(s) 645
PaeI GCATGC 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 998
PagI TCATGA 1 cut(s) 709
PceI AGGCCT 1 cut(s) 943
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 2 cut(s) 924, 1140
PdmI GAANNNNTTC 3 cut(s) 900, 1116, 1230
PfeI GAWTC 1 cut(s) 706
Pfl23II CGTACG 1 cut(s) 186
PfoI TCCNGGA 1 cut(s) 606
PkrI GCNGC 8 cut(s) 38, 41, 62, 276, 320, 667, 856, 877
PleI GAGTC 2 cut(s) 164, 1196
PpsI GAGTC 2 cut(s) 164, 1196
PshBI ATTAAT 1 cut(s) 798
Psp6I CCWGG 1 cut(s) 1372
PspGI CCWGG 1 cut(s) 1372
PspLI CGTACG 1 cut(s) 186
PspN4I GGNNCC 2 cut(s) 35, 1184
PspPI GGNCC 1 cut(s) 955
RsaI GTAC 5 cut(s) 188, 230, 473, 716, 982
RsaNI GTAC 5 cut(s) 187, 229, 472, 715, 981
RseI CAYNNNNRTG 6 cut(s) 28, 68, 645, 885, 1197, 1375
SaqAI TTAA 6 cut(s) 308, 345, 720, 798, 1407, 1487
SatI GCNGC 8 cut(s) 37, 40, 61, 275, 319, 666, 855, 876
Sau3AI GATC 3 cut(s) 637, 1204, 1344
Sau96I GGNCC 1 cut(s) 955
SchI GAGTC 2 cut(s) 164, 1197
ScrFI CCNGG 3 cut(s) 502, 608, 1374
SduI GDGCHC 4 cut(s) 663, 688, 1309, 1450
SfaNI GCATC 4 cut(s) 430, 487, 1312, 1367
SfcI CTRYAG 2 cut(s) 654, 1235
Sfr274I CTCGAG 1 cut(s) 998
SinI GGWCC 1 cut(s) 955
SlaI CTCGAG 1 cut(s) 998
SmiMI CAYNNNNRTG 6 cut(s) 28, 68, 645, 885, 1197, 1375
SmlI CTYRAG 1 cut(s) 998
SmoI CTYRAG 1 cut(s) 998
SphI GCATGC 1 cut(s) 73
Sse9I AATT 7 cut(s) 223, 346, 370, 399, 519, 799, 1488
SseBI AGGCCT 1 cut(s) 943
SsiI CCGC 5 cut(s) 37, 275, 318, 665, 1325
SspMI CTAG 3 cut(s) 383, 1106, 1241
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 3 cut(s) 500, 606, 1372
TaaI ACNGT 2 cut(s) 407, 543
TaiI ACGT 3 cut(s) 344, 720, 750
TaqI TCGA 4 cut(s) 204, 830, 999, 1020
TasI AATT 7 cut(s) 223, 346, 370, 399, 519, 799, 1488
TatI WGTACW 2 cut(s) 228, 980
TauI GCSGC 4 cut(s) 39, 277, 321, 668
TfiI GAWTC 1 cut(s) 706
Tru1I TTAA 6 cut(s) 308, 345, 720, 798, 1407, 1487
Tru9I TTAA 6 cut(s) 308, 345, 720, 798, 1407, 1487
TscAI CASTG 1 cut(s) 548
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 4 cut(s) 39, 60, 854, 875
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 6 cut(s) 512, 531, 698, 893, 1043, 1157
TspGWI ACGGA 2 cut(s) 897, 1324
TspRI CASTG 1 cut(s) 548
VneI GTGCAC 1 cut(s) 1305
VpaK11BI GGWCC 1 cut(s) 955
VspI ATTAAT 1 cut(s) 798
XagI CCTNNNNNAGG 1 cut(s) 1001
XapI RAATTY 2 cut(s) 399, 519
XceI RCATGY 2 cut(s) 46, 73
XhoI CTCGAG 1 cut(s) 998
XmnI GAANNNNTTC 3 cut(s) 900, 1116, 1230
XspI CTAG 3 cut(s) 383, 1106, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.