pycom111g04220

Zinc-finger homeodomain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0015078
Physical Location & Seq
Reverse (-)
2567000 .. 2568055
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g04220.1

Sequence Viewer

Length: 1056 bp
ATGGATATAACCCCGTCCATCACCACCACCACCAACAACACCGCCAGCACAAAATCCCCGGAAGCCGACAGCGAAACTCCGACTCGGATCCGGCAACCCTTAAAGCCTCCGTCCAGCAACGGCGTCCTCAAGCGCCACAACCCCACGCACCACCTCCACCACCACAACATCCCCATCACCCCTGTCGTAGTCACCTACAAAGAATGCCTCAAGAACCACGCCGCTACGCTGGGTGGCCACGCCCTCGACGGCTGCGGCGAGTTCATGCCGTCTCCCGCTGCCGACCCCGCCGACCCCACCTCACTAAAATGCGCTGCATGTGGCTGCCACCGCAATTTTCACCGCCGTGACCCCGAGGACCCCGTGCAGCCAAACACCCCCGCCGCGACAAAGCATGTCATCGAGTACCAACCCCACCACCGCCACCATCCTCCTCCGCCGACTCACCCCGGCAACCGAAGCCCCAGTTCAGCTTCTCCACCGCCGATCTCGTCCTCCTACTACCCCTCCGCCCCCCACATGCTCTTGGCTCTGTCCACCGCTCACGAAAACGCCTTGGCGGGTGCGAATAATAACAACGCCGTTGCTATGCCCCAGATCGTGTCGCCGAGCCCGAATGCGAGGAAGCGGTTCAGGACCAAGTTCACCCAGGACCAGAAGGAGAAGATGTACCAATTCGCAGAGAGGGTCGGGTGGAAGATGCAGAAGAGAGACGAGGAAATCGTGCAGGAGTTCTGTAATGAGGCCGGCATCGAAAAAGGGGTTCTCAAAGTCTGGATGCACAACAATAAGAATACCTTCTCCAAGCGAGACGTGCTCAATGGCGGCGCTGGCGGCCGTGCTGGCAGTTTAAGCAGACCCAGCTTTCTCCTTGAGCATTCCCACCACCACAACAACGGCACCAACGGCAACGGCAACGGCAACGGCAACAACAACGATGATGACGAGGAGGAGGATGATGATCAAAACGACAACAAGAACGGCTTTCCGAATCCGAATCATCATTACCAGGGTGCTGACGGTGGTGGCAACAATGGGTCATCTTCTTCTTCTTGA

Protein Analysis

352

Amino Acids

38.22

Weight (kDa)

6.94

Isoelectric Point (pI)

61.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 64 - 118 1.9e-28 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28920 AT5G15210
fragaria_vesca FvH4_6g48610
malus_domestica MD09G1051200.v1.1 MD17G1051300.v1.1
prunus_persica Prupe.3G267800_v2.0.a1
pyrus_communis pycom111g04220 pycom17g04600
rosa_chinensis RchiOBHm_Chr2g0168471
rosa_laevigata RLG00000021783
rosa_multiflora Rmu_sc0007066.1_g000014
rosa_roxburghii Rroxscaffold_2G00082990
rosa_rugosa Rorug02G0533800
rosa_samantha Rh2AG603800 Rh2CG584800
rosa_wichuraiana Rw2G050060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 899
AccBSI CCGCTC 1 cut(s) 542
AccII CGCG 1 cut(s) 386
AclWI GGATC 2 cut(s) 82, 95
AcoI YGGCCR 2 cut(s) 235, 835
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 2 cut(s) 407, 671
AjiI CACGTC 1 cut(s) 814
AjnI CCWGG 2 cut(s) 648, 1008
AleI CACNNNNGTG 1 cut(s) 345
AluBI AGCT 2 cut(s) 473, 864
AluI AGCT 2 cut(s) 473, 864
Alw21I GWGCWC 1 cut(s) 819
Alw26I GTCTC 3 cut(s) 276, 705, 804
AlwI GGATC 2 cut(s) 82, 95
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 3 cut(s) 235, 744, 835
ApeKI GCWGC 5 cut(s) 252, 278, 314, 324, 367
Asp700I GAANNNNTTC 2 cut(s) 629, 797
AspLEI GCGC 3 cut(s) 135, 314, 830
AspS9I GGNCC 3 cut(s) 358, 636, 652
AsuC2I CCSGG 2 cut(s) 59, 450
AsuHPI GGTGA 6 cut(s) 13, 169, 184, 332, 437, 637
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 3 cut(s) 358, 636, 652
BalI TGGCCA 1 cut(s) 237
BamHI GGATCC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 899
BanII GRGCYC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 819
BbvI GCAGC 5 cut(s) 239, 265, 301, 311, 379
BccI CCATC 3 cut(s) 26, 182, 435
BciT130I CCWGG 2 cut(s) 650, 1010
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 2 cut(s) 59, 450
BcoDI GTCTC 3 cut(s) 276, 705, 804
BfoI RGCGCY 2 cut(s) 136, 831
BglI GCCNNNNNGGC 1 cut(s) 843
Bme1390I CCNGG 4 cut(s) 59, 450, 650, 1010
Bme18I GGWCC 3 cut(s) 358, 636, 652
BmeT110I CYCGRG 1 cut(s) 353
BmgBI CACGTC 1 cut(s) 814
BmgT120I GGNCC 3 cut(s) 358, 636, 652
BmiI GGNNCC 3 cut(s) 89, 360, 901
BmrFI CCNGG 4 cut(s) 59, 450, 650, 1010
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 3 cut(s) 690, 759, 768
BmuI ACTGGG 1 cut(s) 459
BplI GAGNNNNNCTC 2 cut(s) 801, 833
BpuEI CTTGAG 3 cut(s) 113, 194, 893
BpuMI CCSGG 2 cut(s) 59, 450
BsaBI GATNNNNATC 1 cut(s) 960
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 6 cut(s) 57, 354, 448, 555, 648, 1009
BsaXI ACNNNNNCTCC 4 cut(s) 491, 521, 653, 683
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 1 cut(s) 465
Bse8I GATNNNNATC 1 cut(s) 960
BseBI CCWGG 2 cut(s) 650, 1010
BseDI CCNNGG 6 cut(s) 57, 354, 448, 555, 648, 1009
BseGI GGATG 4 cut(s) 168, 427, 783, 961
BseJI GATNNNNATC 1 cut(s) 960
BseNI ACTGG 1 cut(s) 465
BseRI GAGGAG 3 cut(s) 423, 962, 965
BseX3I CGGCCG 1 cut(s) 835
BseXI GCAGC 5 cut(s) 239, 265, 301, 311, 379
BseYI CCCAGC 2 cut(s) 229, 860
BsgI GTGCAG 2 cut(s) 386, 746
Bsh1236I CGCG 1 cut(s) 386
Bsh1285I CGRYCG 1 cut(s) 838
BshFI GGCC 3 cut(s) 237, 746, 837
BshNI GGYRCC 1 cut(s) 899
BsiEI CGRYCG 1 cut(s) 838
BsiHKAI GWGCWC 1 cut(s) 819
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 4 cut(s) 59, 91, 450, 747
BsmAI GTCTC 3 cut(s) 276, 705, 804
BsmBI CGTCTC 3 cut(s) 276, 705, 804
BsmI GAATGC 3 cut(s) 209, 622, 877
BsnI GGCC 3 cut(s) 237, 746, 837
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 2 cut(s) 614, 819
Bsp143I GATC 4 cut(s) 87, 486, 597, 961
BspANI GGCC 3 cut(s) 237, 746, 837
BspFNI CGCG 1 cut(s) 386
BspLI GGNNCC 3 cut(s) 89, 360, 901
BspPI GGATC 2 cut(s) 82, 95
BspT107I GGYRCC 1 cut(s) 899
BsrBI CCGCTC 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 746
BsrI ACTGG 1 cut(s) 465
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 6 cut(s) 57, 354, 448, 555, 648, 1009
BssMI GATC 4 cut(s) 87, 486, 597, 961
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 2 cut(s) 650, 1010
Bst4CI ACNGT 1 cut(s) 1022
Bst6I CTCTTC 1 cut(s) 701
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 4 cut(s) 46, 748, 832, 844
BstF5I GGATG 4 cut(s) 168, 427, 783, 961
BstFNI CGCG 1 cut(s) 386
BstH2I RGCGCY 2 cut(s) 136, 831
BstHHI GCGC 3 cut(s) 135, 314, 830
BstKTI GATC 4 cut(s) 90, 489, 600, 964
BstMAI GTCTC 3 cut(s) 276, 705, 804
BstMBI GATC 4 cut(s) 87, 486, 597, 961
BstMCI CGRYCG 1 cut(s) 838
BstNI CCWGG 2 cut(s) 650, 1010
BstNSI RCATGY 3 cut(s) 321, 398, 523
BstSCI CCNGG 4 cut(s) 57, 448, 648, 1008
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 5 cut(s) 239, 265, 301, 311, 379
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BstZI CGGCCG 1 cut(s) 835
BsuRI GGCC 3 cut(s) 237, 746, 837
BtrI CACGTC 1 cut(s) 814
BtsCI GGATG 4 cut(s) 168, 427, 783, 961
Cac8I GCNNGC 4 cut(s) 46, 748, 832, 844
CfoI GCGC 3 cut(s) 135, 314, 830
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 3 cut(s) 358, 636, 652
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 406, 670
CviAII CATG 4 cut(s) 265, 318, 395, 520
CviQI GTAC 2 cut(s) 406, 670
DpnI GATC 4 cut(s) 89, 488, 599, 963
DpnII GATC 4 cut(s) 87, 486, 597, 961
EaeI YGGCCR 2 cut(s) 235, 835
EagI CGGCCG 1 cut(s) 835
Eam1104I CTCTTC 1 cut(s) 701
EarI CTCTTC 1 cut(s) 701
EciI GGCGGA 2 cut(s) 426, 499
EclXI CGGCCG 1 cut(s) 835
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 614
Eco47I GGWCC 3 cut(s) 358, 636, 652
Eco52I CGGCCG 1 cut(s) 835
Eco88I CYCGRG 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 648, 1008
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 555
Esp3I CGTCTC 3 cut(s) 276, 705, 804
FaeI CATG 4 cut(s) 268, 321, 398, 523
FaiI YATR 6 cut(s) 8, 266, 319, 396, 521, 590
FalI AAGNNNNNCTT 4 cut(s) 782, 814, 968, 1000
FatI CATG 4 cut(s) 264, 317, 394, 519
FauI CCCGC 4 cut(s) 283, 295, 388, 553
FbaI TGATCA 1 cut(s) 961
FokI GGATG 4 cut(s) 155, 414, 790, 968
FriOI GRGCYC 1 cut(s) 614
GlaI GCGC 3 cut(s) 134, 313, 829
GsaI CCCAGC 2 cut(s) 233, 864
HaeII RGCGCY 2 cut(s) 136, 831
HaeIII GGCC 3 cut(s) 237, 746, 837
HapII CCGG 4 cut(s) 59, 91, 450, 747
HgaI GACGC 1 cut(s) 112
HhaI GCGC 3 cut(s) 135, 314, 830
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 4 cut(s) 268, 321, 398, 523
Hin6I GCGC 3 cut(s) 133, 312, 828
HinP1I GCGC 3 cut(s) 133, 312, 828
HinfI GANTC 4 cut(s) 82, 442, 991, 997
HpaII CCGG 4 cut(s) 59, 91, 450, 747
HphI GGTGA 6 cut(s) 13, 169, 184, 332, 437, 637
Hpy166II GTNNAC 2 cut(s) 537, 645
Hpy188I TCNGA 4 cut(s) 81, 87, 990, 996
Hpy188III TCNNGA 5 cut(s) 211, 545, 634, 775, 1053
Hpy8I GTNNAC 2 cut(s) 537, 645
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 2 cut(s) 652, 808
HpyCH4III ACNGT 1 cut(s) 1022
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 5 cut(s) 317, 367, 703, 727, 781
HpySE526I ACGT 1 cut(s) 813
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 4 cut(s) 268, 321, 398, 523
HspAI GCGC 3 cut(s) 133, 312, 828
KroI GCCGGC 1 cut(s) 746
KroNI GCCGGC 1 cut(s) 748
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 4 cut(s) 87, 486, 597, 961
Lsp1109I GCAGC 5 cut(s) 239, 265, 301, 311, 379
LweI GCATC 3 cut(s) 690, 759, 768
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 2 cut(s) 190, 347
MalI GATC 4 cut(s) 89, 488, 599, 963
MbiI CCGCTC 1 cut(s) 542
MboI GATC 4 cut(s) 87, 486, 597, 961
MboII GAAGA 6 cut(s) 676, 709, 718, 1035, 1038, 1041
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 2 cut(s) 614, 819
MlsI TGGCCA 1 cut(s) 237
MluCI AATT 2 cut(s) 334, 674
MluNI TGGCCA 1 cut(s) 237
MlyI GAGTC 2 cut(s) 76, 436
MmeI TCCRAC 1 cut(s) 104
Mox20I TGGCCA 1 cut(s) 237
MroNI GCCGGC 1 cut(s) 746
MroXI GAANNNNTTC 2 cut(s) 629, 797
MscI TGGCCA 1 cut(s) 237
MseI TTAA 2 cut(s) 101, 851
MslI CAYNNNNRTG 2 cut(s) 307, 345
Msp20I TGGCCA 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 4 cut(s) 59, 91, 450, 747
MspR9I CCNGG 4 cut(s) 59, 450, 650, 1010
Mva1269I GAATGC 3 cut(s) 209, 622, 877
MvaI CCWGG 2 cut(s) 650, 1010
MvnI CGCG 1 cut(s) 386
NaeI GCCGGC 1 cut(s) 748
NciI CCSGG 2 cut(s) 59, 450
NdeII GATC 4 cut(s) 87, 486, 597, 961
NgoMIV GCCGGC 1 cut(s) 746
NlaIII CATG 4 cut(s) 268, 321, 398, 523
NlaIV GGNNCC 3 cut(s) 89, 360, 901
NmeAIII GCCGAG 1 cut(s) 633
NmuCI GTSAC 2 cut(s) 190, 347
NspI RCATGY 3 cut(s) 321, 398, 523
OliI CACNNNNGTG 1 cut(s) 345
PcsI WCGNNNNNNNCGW 4 cut(s) 255, 611, 720, 942
PctI GAATGC 3 cut(s) 209, 622, 877
PdiI GCCGGC 1 cut(s) 748
PdmI GAANNNNTTC 2 cut(s) 629, 797
PfeI GAWTC 2 cut(s) 991, 997
PleI GAGTC 2 cut(s) 76, 436
PpsI GAGTC 2 cut(s) 76, 436
PpuMI RGGWCCY 1 cut(s) 358
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 648, 1008
PspFI CCCAGC 2 cut(s) 229, 860
PspGI CCWGG 2 cut(s) 648, 1008
PspN4I GGNNCC 3 cut(s) 89, 360, 901
PspPI GGNCC 3 cut(s) 358, 636, 652
PspPPI RGGWCCY 1 cut(s) 358
PsuI RGATCY 1 cut(s) 87
RsaI GTAC 2 cut(s) 407, 671
RsaNI GTAC 2 cut(s) 406, 670
RseI CAYNNNNRTG 2 cut(s) 307, 345
SaqAI TTAA 2 cut(s) 101, 851
Sau3AI GATC 4 cut(s) 87, 486, 597, 961
Sau96I GGNCC 3 cut(s) 358, 636, 652
SchI GAGTC 2 cut(s) 76, 436
ScrFI CCNGG 4 cut(s) 59, 450, 650, 1010
SduI GDGCHC 2 cut(s) 614, 819
SetI ASST 7 cut(s) 156, 197, 302, 475, 800, 816, 866
SfaNI GCATC 3 cut(s) 690, 759, 768
SinI GGWCC 3 cut(s) 358, 636, 652
SmiMI CAYNNNNRTG 2 cut(s) 307, 345
SmlI CTYRAG 3 cut(s) 128, 209, 872
SmoI CTYRAG 3 cut(s) 128, 209, 872
Sse9I AATT 2 cut(s) 334, 674
StyD4I CCNGG 4 cut(s) 57, 448, 648, 1008
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 1 cut(s) 1022
TaiI ACGT 1 cut(s) 816
TaqI TCGA 3 cut(s) 246, 402, 753
TasI AATT 2 cut(s) 334, 674
TauI GCSGC 5 cut(s) 224, 258, 386, 828, 837
TfiI GAWTC 2 cut(s) 991, 997
Tru1I TTAA 2 cut(s) 101, 851
Tru9I TTAA 2 cut(s) 101, 851
TseFI GTSAC 2 cut(s) 190, 347
TseI GCWGC 5 cut(s) 252, 278, 314, 324, 367
Tsp45I GTSAC 2 cut(s) 190, 347
TspDTI ATGAA 1 cut(s) 253
TspGWI ACGGA 1 cut(s) 99
VpaK11BI GGWCC 3 cut(s) 358, 636, 652
XceI RCATGY 3 cut(s) 321, 398, 523
XmnI GAANNNNTTC 2 cut(s) 629, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.