pycom11g00180

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010441
Physical Location & Seq
Forward (+)
160186 .. 162981
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 900 bp
ATGGAGGGAAGCTTGCTTCTCAGTCTCGGCTGCTCCTCTTGTTCTCCCCATTTAAAACCTCATTTCTCTTTCTCCTCCTCTTCTTCCTTGCTCTTCGGAAAATCCCACCTTCTGGGTTTAATCCCCAGACCCTTACTCGCCCACAGCACCCTATCACTCCCCTCCCCTCGAACCGCCCTCCGTACTTCGTCCTTCCCTCTCGACCAAGCTCTCGAAAAGCCCAGCAATGGCTCAGCAGATACGTTGCCCAAGATTGATAAGAGTGGCAGATTTTGCAGCCCCAGAGCCGCCAGAGAGCTCGCTCTGTCGATTGTTTATGCTTCTTGTTTAGAGGGTTCTGACCCAATACGTTTATTTGAAAAGCGAATAAATGTCCGTCGAGAACCCGGGTATGAATTTGACAGGGCATCATTACTTGAATATAATCCCATGAGCTTCGGAGGACCCCCTGTCACAGTTGAAACCGTTGAGGAAGCAGATGAGCTCCTGCGCAAAGATGAAAAGGAATCTGCAATTGAAGCAGAAGTTCTTGCAGCTCCTCCAAAATTGGTATACAGCAAACTGATTTTACGATTTACAAGGAAACTTTTGGTTGCAGTCATGGATAAATGGGACAGTCATGTGCTCGTTATTGACAACGTTGCCCCTCCAAATTGGAAGGATGAGCCAGCAGGCAGAATATTGGAGCTTTGCATCCTCCACTTGGCAATGTCAGAAATAACAGTGCTCGAAACAAGACACCCGATTGTCATTAACGAGGCCGTAGATCTTGCTAAACGGTTCTGTGATGGATCAGCACCCCGTATTATCAATGGTTGTCTTAGAACATTTGTCAAACAAATTGAAGAAGCTGGTGGTCTAACTCGGGCTTTGGGGTATAAACAGAATGTGGTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

300

Amino Acids

32.91

Weight (kDa)

7.05

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusB PF01029 218 - 279 6e-09 NusB family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 491
AccB7I CCANNNNNTGG 1 cut(s) 112
AccI GTMKAC 1 cut(s) 552
AciI CCGC 2 cut(s) 174, 288
AclI AACGTT 1 cut(s) 639
AclWI GGATC 1 cut(s) 799
AcsI RAATTY 1 cut(s) 395
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 3 cut(s) 112, 227, 703
AgsI TTSAA 5 cut(s) 359, 419, 461, 518, 845
AhdI GACNNNNNGTC 1 cut(s) 449
AluBI AGCT 8 cut(s) 12, 209, 298, 435, 484, 536, 688, 851
AluI AGCT 8 cut(s) 12, 209, 298, 435, 484, 536, 688, 851
Alw21I GWGCWC 4 cut(s) 300, 486, 627, 729
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 799
Ama87I CYCGRG 2 cut(s) 386, 864
AoxI GGCC 1 cut(s) 759
ApeKI GCWGC 3 cut(s) 30, 276, 533
ApoI RAATTY 1 cut(s) 395
AspLEI GCGC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 443
AsuC2I CCSGG 2 cut(s) 387, 388
AvaI CYCGRG 2 cut(s) 386, 864
AvaII GGWCC 1 cut(s) 443
BanII GRGCYC 2 cut(s) 300, 486
Bbv12I GWGCWC 4 cut(s) 300, 486, 627, 729
BbvI GCAGC 3 cut(s) 17, 288, 545
BccI CCATC 1 cut(s) 782
BceAI ACGGC 1 cut(s) 746
BcnI CCSGG 2 cut(s) 387, 388
BcoDI GTCTC 1 cut(s) 29
BglII AGATCT 1 cut(s) 766
BisI GCNGC 4 cut(s) 31, 277, 288, 534
BlpI GCTNAGC 1 cut(s) 232
BlsI GCNGC 4 cut(s) 32, 278, 289, 535
Bme1390I CCNGG 2 cut(s) 387, 388
Bme18I GGWCC 1 cut(s) 443
BmeRI GACNNNNNGTC 1 cut(s) 449
BmeT110I CYCGRG 2 cut(s) 386, 864
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 2 cut(s) 387, 388
BmsI GCATC 2 cut(s) 416, 702
Bpu1102I GCTNAGC 1 cut(s) 232
BpuMI CCSGG 2 cut(s) 387, 388
BsaJI CCNNGG 1 cut(s) 386
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 227, 703
Bse3DI GCAATG 2 cut(s) 232, 714
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 2 cut(s) 667, 693
BseLI CCNNNNNNNGG 3 cut(s) 112, 227, 703
BseMI GCAATG 2 cut(s) 232, 714
BseMII CTCAG 2 cut(s) 34, 246
BseRI GAGGAG 4 cut(s) 25, 64, 67, 528
BseXI GCAGC 3 cut(s) 17, 288, 545
BseYI CCCAGC 1 cut(s) 221
BshFI GGCC 1 cut(s) 761
BsiHKAI GWGCWC 4 cut(s) 300, 486, 627, 729
BsiHKCI CYCGRG 2 cut(s) 386, 864
BsiSI CCGG 1 cut(s) 387
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 3 cut(s) 112, 227, 703
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 1 cut(s) 761
BsoBI CYCGRG 2 cut(s) 386, 864
Bsp1286I GDGCHC 4 cut(s) 300, 486, 627, 729
Bsp143I GATC 2 cut(s) 766, 791
Bsp1720I GCTNAGC 1 cut(s) 232
BspACI CCGC 2 cut(s) 174, 288
BspANI GGCC 1 cut(s) 761
BspCNI CTCAG 2 cut(s) 33, 245
BspLI GGNNCC 1 cut(s) 445
BspPI GGATC 1 cut(s) 799
BspQI GCTCTTC 1 cut(s) 98
BsrDI GCAATG 2 cut(s) 232, 714
BssECI CCNNGG 1 cut(s) 386
BssMI GATC 2 cut(s) 766, 791
BssNAI GTATAC 1 cut(s) 553
Bst1107I GTATAC 1 cut(s) 553
Bst4CI ACNGT 5 cut(s) 457, 466, 617, 724, 780
Bst6I CTCTTC 2 cut(s) 85, 98
BstAPI GCANNNNNTGC 1 cut(s) 273
BstC8I GCNNGC 4 cut(s) 14, 300, 669, 673
BstDEI CTNAG 3 cut(s) 20, 232, 821
BstF5I GGATG 2 cut(s) 667, 693
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 2 cut(s) 769, 794
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 2 cut(s) 766, 791
BstMWI GCNNNNNNNGC 2 cut(s) 273, 518
BstSCI CCNGG 2 cut(s) 385, 386
BstV1I GCAGC 3 cut(s) 17, 288, 545
BstX2I RGATCY 1 cut(s) 766
BstYI RGATCY 1 cut(s) 766
BstZ17I GTATAC 1 cut(s) 553
BsuRI GGCC 1 cut(s) 761
BtsCI GGATG 2 cut(s) 667, 693
BtsIMutI CAGTG 1 cut(s) 729
Cac8I GCNNGC 4 cut(s) 14, 300, 669, 673
CfoI GCGC 1 cut(s) 492
Cfr13I GGNCC 1 cut(s) 443
Cfr9I CCCGGG 1 cut(s) 386
Csp6I GTAC 1 cut(s) 183
CviAII CATG 3 cut(s) 430, 601, 620
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 3 cut(s) 20, 232, 821
DpnI GATC 2 cut(s) 768, 793
DpnII GATC 2 cut(s) 766, 791
DraI TTTAAA 1 cut(s) 54
DriI GACNNNNNGTC 1 cut(s) 449
Eam1104I CTCTTC 2 cut(s) 85, 98
Eam1105I GACNNNNNGTC 1 cut(s) 449
EarI CTCTTC 2 cut(s) 85, 98
Ecl136II GAGCTC 2 cut(s) 298, 484
Eco24I GRGCYC 2 cut(s) 300, 486
Eco47I GGWCC 1 cut(s) 443
Eco53kI GAGCTC 2 cut(s) 298, 484
Eco88I CYCGRG 2 cut(s) 386, 864
EcoICRI GAGCTC 2 cut(s) 298, 484
EcoO109I RGGNCCY 1 cut(s) 443
EcoT38I GRGCYC 2 cut(s) 300, 486
FaeI CATG 3 cut(s) 433, 604, 623
FaiI YATR 8 cut(s) 318, 393, 423, 431, 553, 602, 621, 879
FaqI GGGAC 1 cut(s) 626
FatI CATG 3 cut(s) 429, 600, 619
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 4 cut(s) 31, 277, 288, 534
FokI GGATG 2 cut(s) 674, 680
FriOI GRGCYC 2 cut(s) 300, 486
Fsp4HI GCNGC 4 cut(s) 31, 277, 288, 534
FspI TGCGCA 1 cut(s) 491
GlaI GCGC 1 cut(s) 491
GluI GCNGC 4 cut(s) 31, 277, 288, 534
GsaI CCCAGC 1 cut(s) 225
HaeIII GGCC 1 cut(s) 761
HapII CCGG 1 cut(s) 387
HhaI GCGC 1 cut(s) 492
Hin1II CATG 3 cut(s) 433, 604, 623
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 1 cut(s) 506
HpaII CCGG 1 cut(s) 387
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 4 cut(s) 98, 340, 440, 715
Hpy188III TCNNGA 3 cut(s) 200, 212, 380
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 3 cut(s) 119, 202, 652
HpyCH4III ACNGT 5 cut(s) 457, 466, 617, 724, 780
HpyCH4IV ACGT 3 cut(s) 242, 349, 639
HpyCH4V TGCA 5 cut(s) 276, 512, 533, 596, 693
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 518
HpyF3I CTNAG 3 cut(s) 20, 232, 821
HpySE526I ACGT 3 cut(s) 242, 349, 639
Hsp92II CATG 3 cut(s) 433, 604, 623
HspAI GCGC 1 cut(s) 490
Kzo9I GATC 2 cut(s) 766, 791
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 4 cut(s) 38, 489, 541, 685
Lsp1109I GCAGC 3 cut(s) 17, 288, 545
LweI GCATC 2 cut(s) 416, 702
MaeII ACGT 3 cut(s) 242, 349, 639
MaeIII GTNAC 2 cut(s) 451, 892
MalI GATC 2 cut(s) 768, 793
MboI GATC 2 cut(s) 766, 791
MboII GAAGA 4 cut(s) 72, 75, 85, 857
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 1 cut(s) 766
MhlI GDGCHC 4 cut(s) 300, 486, 627, 729
MluCI AATT 5 cut(s) 395, 513, 545, 652, 840
MseI TTAA 3 cut(s) 53, 119, 753
MspI CCGG 1 cut(s) 387
MspR9I CCNGG 2 cut(s) 387, 388
MunI CAATTG 1 cut(s) 513
MwoI GCNNNNNNNGC 2 cut(s) 273, 518
NciI CCSGG 2 cut(s) 387, 388
NdeII GATC 2 cut(s) 766, 791
NlaIII CATG 3 cut(s) 433, 604, 623
NlaIV GGNNCC 1 cut(s) 445
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 451, 892
NsbI TGCGCA 1 cut(s) 491
PciSI GCTCTTC 1 cut(s) 98
PfeI GAWTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 4 cut(s) 32, 278, 289, 535
PpuMI RGGWCCY 1 cut(s) 443
Psp124BI GAGCTC 2 cut(s) 300, 486
Psp1406I AACGTT 1 cut(s) 639
Psp5II RGGWCCY 1 cut(s) 443
PspFI CCCAGC 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 1 cut(s) 443
PspPPI RGGWCCY 1 cut(s) 443
PsuI RGATCY 1 cut(s) 766
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
SacI GAGCTC 2 cut(s) 300, 486
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 3 cut(s) 53, 119, 753
SatI GCNGC 4 cut(s) 31, 277, 288, 534
Sau3AI GATC 2 cut(s) 766, 791
Sau96I GGNCC 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 387, 388
SduI GDGCHC 4 cut(s) 300, 486, 627, 729
SfaNI GCATC 2 cut(s) 416, 702
SinI GGWCC 1 cut(s) 443
SmaI CCCGGG 1 cut(s) 388
Sse9I AATT 5 cut(s) 395, 513, 545, 652, 840
SsiI CCGC 2 cut(s) 174, 288
SspI AATATT 1 cut(s) 681
SstI GAGCTC 2 cut(s) 300, 486
StyD4I CCNGG 2 cut(s) 385, 386
TaaI ACNGT 5 cut(s) 457, 466, 617, 724, 780
TaiI ACGT 3 cut(s) 245, 352, 642
TaqI TCGA 6 cut(s) 169, 201, 213, 308, 379, 729
TasI AATT 5 cut(s) 395, 513, 545, 652, 840
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 3 cut(s) 53, 119, 753
Tru9I TTAA 3 cut(s) 53, 119, 753
TscAI CASTG 1 cut(s) 729
TseFI GTSAC 2 cut(s) 451, 892
TseI GCWGC 3 cut(s) 30, 276, 533
Tsp45I GTSAC 2 cut(s) 451, 892
TspDTI ATGAA 2 cut(s) 408, 513
TspGWI ACGGA 2 cut(s) 170, 365
TspMI CCCGGG 1 cut(s) 386
TspRI CASTG 1 cut(s) 729
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 1 cut(s) 395
XmaI CCCGGG 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.