Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0006814

Physical Location & Seq

Genomic Coordinates Reverse (-)

2787648 .. 2800050

Gene Structure 12403 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5679 bp

ATGATCAGTCACCCCCCTCCTTCATCTTTCACACAAGCCAGAAAGAAAACCCCAATCCGTCTCCCTCAACTCTCTCTCTCAATTTCCCAACTTTCTTGTTCAGAAAGGCAGGCTGAACCCAAAGTCTTCGAATGCTTTCACTATAAATCCCCACAAACTCACTCTCAAATCCATTCCCTTTCTCTTCTGGATCACCAATTCACAGAGCAAATCCTGCAAACTATGCATCTCTACAATGCATGGCTGCCGCCTCCGGTGGCCGAGGAGTCGAAGAAGGAGAAGGAGTCCTTCTCTTGGGTCGTCTCCTCCGTCAAGAACTCGTACAAACACGACGATCCTGAATCCGTCTATGCCACTCTCAAATGGGTTTCTGTTATCGACCTTTTCCTCAAGGCGAAAAGTGAAGTATCCTTGGAAGATGTAACTGTTCTTGTTGAATTTGGATTGGAATTGTTTTATGTATCGCAAAATAAGCTTTATGCCCAGGTTAGATGGGGAAACATCCTAGTGAAACTACTCAATAAGCATCGAAAGAAGTTGTCTTTGAAATTAAAATGGCGGCCTTTGTACGAAACATTGATCCATACACATTTCACTAGGAATACAGGTCCAGAAGGCTGGAGATTGAGACAACGACATTTTGAGGCTACTACTTCTCTTGTTCGGTCTTGCCGGAAGTTCTTCCCACCAGGTTCTGCCTTTGAGATATGGTCCGAGTTTAGATCTTTATTGGAAAATCCCTGGCATAATTCATCCTTCGAGGGTTCTGGGTTTGTTAGACTGTTTCTTCCTACAAACTTGGACAACCAGGACTTCTTTTCGCACGATTGGATTAAAGAGTTTTTCCACCTCTGGGACTCGATACCCAATTGCCAATTTTGGAACAGTCAATGGGCAGCTGTTATAGCTCGTGTTGTAAAGAACTACAAGTTGATTGATTGGGAGTGTTACCTGCCTGCACTTTTCACCAGATACTTAAATATGTTTGAGGTTCCTGTGGCAAATGGAAGTGGATCATATCCATTTTCTGTGGATGTTCCAAGATACACAAGATTCTTGTTCTCCAATAAGACAGTTACCCCAGCAAAGGCCATCGCAAAGACCATTGTCTATTTGCTAAGGCCTGGTAGCTCAGCACAGGAGCATTTTGAAAAATTGGTCAACCTCTTAGAACAATATTACCACCCTTCTAATGGTGGTCGCTGGACTTATGCATTAGAACGCTTTTTGCTCTATTTGGTAATTTCATTCCGGAAACGCTTACAGCATGAACAACTGAGCATTGATAAAGATAGACAGGATGAACAGTACCTAGGAAGATCAGAAAGGAATTTCTTTGTCAGGGTGGTGCTTAAGTTAATTGATCGATCATCATCTGGTCAATATAGCAAGAATGAACATCTTTCTGAAACAGTTGCTGCTGCAACATCGATTTTGTCTTATGTGGAGCCTACTTTGGTCCTTCCTTTTGTCGCATCTCGCTTCCAAATGGCCTTGGAGACGATGACTGCCACCCACCAGTTGCAGATTGCAGTGACGTCGGTAGCATTTGTTGGGCGTTCATTGTTCCTCACGTCCCTATCAGATTCTGCAGTAAAATCAGTTGATCCTGGTAATGGTGATGAATTCATTGAGCTTTTGATGGTTTCACTATCTAATGCATTACTTGGTATGGATGCCAATGATCCTCCTAAAACCTTGGCAACAATGCAATTAATTGGCTCTATATTTTCCAATATGTCTTCATTGGATGATAATATTGATGAGCTATCTGTCATGCCAATGATTCGGTTTTCTGAATGGTTAGATGAGTTCTTGTGTCGCCTATTTTCCTTACTCTTACACCTGGAACCAAGCAGTGTTACGAATGAAGGCCTACATTCATCGGCAGCGTCAGGAACTTTTCTAGTTGAGGATGGTCCTTACTACTATTGCATGCTTGAAATCTTGCTCGGGAGACTTTCACAACCTCTCTATAATCAGGCTTTGAAGAAGATCTCCAAGTTTGTGAAGACAAATATTCTTCCAGGGGCAATTGCAGAGGTTGGACTGCTTTGTTGCGCATGTGTTCATTCCAACCCAGAAGAAGCAGTTACTCAACTTGTCAAGCCAATTTTATTGTCTGTTATTTCTTCTTTGGAAGGAACACCAGCTACAGGATTTGGAGGTAGAGGAATATGTGGCGCTTCTGTTTCAACTAAGGTTAAGCCAACAATTTCTCCAGCTCTTGAAACAGCAATCGACTATCAATTAAAAGTATTATCAGTTGCCATAAGTTATGGAGGTCCTGCACTTCTGCGCTACAAGGATCACTTCAAAGAGGCTATACTTTCAGCTTTTGAGTCCCCTTCTTGGAAGGTCAATGGAGCTGGTGATCATCTTCTTCGATCTCTCCTTGGAAGCCTGGTTCTTTATTATCCTATTGATCAGTACAAGTGTGTTTTGCATCATCCAAATGCTGCTGCATTAGAGGAATGGATCAACACCAAAGATTATTCCAATGATAAACCATTGGCGGCCCCCAAGTGGCATATCCCAAGTGTCGAAGAAGTTGAATCTGCAAATGAACTTTTGGACTGTCATTTTCGGATGGCTTTGGATGATCTTTCGAGAATATGTGAAACTAAAATCCACTCAGATCCAGGAGATGAGAAAGAGCACTTGAAAGTGACTCTTCTGCGTATAGATTCTTCATTGCAAGGTGTTTTGTCTTGCCTGCCTGACTTCGCCCCATCCTCAAGGAATGGGACCCTTGAACATCCAAATCATGCTGCTTTTCTAATAGCCGGAGCAACAGGTTCAACTGTTGGTACCACTAAATTGCGAGAAAAAGCTGCTGACATTATACATGCAGCCTGCAAATACATACTAGAGAAGAAATCAGATGATAGCATCCTGTTGATACTCATTATTCGTATTATGGATGCTTTAGGAAACTATGGAAGTTTGGAGTATGATGAGTGGTCAAATCACCGGCAGGCATGGAAACTAGAATCTGCTGCCATTGTAGAACCTTCAGTAAACTTTATAAATTCTGCTCATTCGAAGGGGAAGAGAAGGCCGCGGTGGGCACTCATTGACAAGGCATTTATGCACAGTACATGGAGATCATCACAATCATCTTATCATATATACCGTACTAATGGGAATTTTGGTCCACCAGACCATGTGAATCTTTTGGTGGATAATCTTCTCAATCTCTCATTGCATAGCTATGAAACTGTTCGTTTACTTGCTGGGAAATCTCTTTTAAAGATGATAAAGAGATGGCCATCTATGATTTCAAAGTGCGTCCTTTCTCTAACAGAGAACTTACGGAATCCCAAGTCACCAGAATATATGGTGCTAGGTTCATGTGCAGTCCTTGCCTCACAGACAGTTCTCAAGCATTTGACAATGGACCCAAAGGCGTTCTCTTCGTGCATTCTTGGAATTCTTTCAAGCTCTCATCATGAATCACTGAAATCCCAGAAAGCAATCAATGAGCTTTTTGTCAAATACAACATTCACTTTGCTGGAGTATCTAGAAGCATTTTCAGGGCATCAGGCAATAATACAGAAGCACCAGATTTTGCAGATTTGGTTTCTCAGATCACTTCTATGAGTTTTGATTCTACTGGCTTGCATTGGAGGTACAATCTGATGGCTAATAGGGTTCTACTCTTGTTGGCAATGTCATCTAGGAATGACCCAACTTCCTCCTCAAAAATTTTGAGTGAAACTGCCGGTCACTTCTTAAAGAATTTAAAAAGTCAGCTTCCTCAGACAAGGATACTTGCAATTTCTGCACTAAATACACTGTTAAAAGAATCACCTTATAAACTATCTGCTGGGGAACAGACTCTCCCCTCTGGGAATGTGCAAGGAAGTAGAAAGTCATCCCTTGAAGGAGAATTAACCCAAATATTTCAGGAAGATGGTTTTTTCAATGAGACCTTGAACAGTCTTTCTCATGTTCATATTGTTACTGATACTGAAAGCGCATCTTCCAGAGGCAATCATGGAAGTTCTTTTCAGAGCCTCGCAGACAAATCAATCACCCGATTTTATTTTGATTTTTCTGCTTCATGGCCACGCACTCCTACTTGGATCTCTTTATTAGGGAGTGACACATTTTACTCGAACTTTGCTAGAATTTTCAAGCGGCTAATACAGGAATGTGGCATGCCTGTTCTATTGGCACTTAAAAGCTCATTAGAGGAGTTTGCAAATGCCAAGGAGAGGTCTAAGCAGTGTGTTGCTGCTGAAGCATTGGCTGGAATATTGCATTCTGATGTTGACGGTATTTTAGTAGCATGGGATACTTGGATTTTGGCCCAGTTGCAGAATATAATCTTATCACAATCGGTAGAGTCAATACCTGAGTGGGCAGCCTGTATACGTTACGCTGTTACTGGGAAAGGGAAGCATGGAACAAGAGTTCCTCTTTTGAGGCAACCAGTATTGGATTGTTTGGTCACACCTTTACCTGGAACTGTAACCACTACTGTAGTTGCCAAGCGCTATGCTTTTCTTTCAGCTGCACTTATAGAATTATCTCCACAGACAATGCCATCAACTGAAGTACAGCTCCATCATAGACTTCTGGAAGAGCTGCTTGGTAGCATGTGCCATTCATCTGCCCAAGTAAGAGAAGCTTTAGGTGTTACTCTTTCAGTGGTGTGCTCTAACATCCAGCTTTATGAGTCATTTAATCATGAGCATTCACTTGAGGGCGAAAAAAGAGATGTTGCCAACAAATTTGATGGGAAAAGTTGGGTTCAGTTTCTGAAAGAGCGAGCATCTGAAGTGCTGATGAGTATTCAGAATACTTCCCAGTCTGACAGTTTGGATACCGCAACAAATATTAGTTCTGAGAATGGGAATTCAAATGGTGATTCACAGGATGATGTCAAGTGGATGGAGACGCTTTTCCATTTTATCATCTCATCTTTGAAGTCTGGCCGAGTTTCATATCTTCTGGATGTAATTGTGGGACTTCTTTCTCCTGTTATATCCTTACAGGAAACTTCAAGTAAAGATCTATCAACCTTAGCAAAAGCAGCTTTTGAATTGCTGAAGTGGAGAATTTTCTGGGGACCTCATCTCCAGGAGGCTGTATCTGTGATTCTTTCTTCTGCCAATGACTCTAACTGGCGAATCAGATCTGCAACACTCACATATCTGCGAACTTTTATGTATAGGCACACTTTTGTCCTCTCAAGTGCGGAGAAGCAGCAAATTTGGAGAACTGTTGAAAATCTACTTTTAGACAACCAAGTTGAGGTAAGAGAGCATGCTGCTGCAGTTTTAGCAGGCCTAATGAAGGGTGGGGATGAAGATCTTGCTAAAGACTTTCGCGATAAAGCTTATACCAAAGCTACTACTCTTCAGAGGAAGAGAAAGCAGAGAACGTTGAGCTCGAGCCAGTCCATAGCTTCTATTCACGGTGCTGTACTTGCTTTGGTGGCTTCTGTGTTGTCAGCCCCTTATGATATGCCCAGTTGGTTGCCGGAACATGTCACTTTACTCGCTCGTTTTAGTGGAGAGCCATCGCCGGTGAAATCTACGGTTACAAAAGCAGTTGCAGAGTTTCGACGTACCCATGCTGATACATGGAATATTCAGAAAGATTCATTTACTGAAGAGCAACTTGAGGTTCTGGCTGATACGTCGTCTTCATCGTCGTATTTTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005839 GO:0006508 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010498 GO:0010499 GO:0010604 GO:0010952 GO:0016504 GO:0019222 GO:0019538 GO:0030162 GO:0030163 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032991 GO:0042393 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051603 GO:0052547 GO:0060255 GO:0061134 GO:0065007 GO:0065009 GO:0070577 GO:0070628 GO:0071704 GO:0080090 GO:0098772 GO:0140030 GO:0140033 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

BLM10_mid DUF3437

Protein Analysis

1893

Amino Acids

212.07

Weight (kDa)

7.16

Isoelectric Point (pI)

45.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEAT_PSME4_mid	PF16507	384 - 581	7.2e-26	Proteasome activator complex subunit 4, mid HEAT repeats region
HEAT_PSME4_mid	PF16507	583 - 902	1.5e-18	Proteasome activator complex subunit 4, mid HEAT repeats region
PSME4_C	PF11919	1807 - 1892	4e-28	Proteasome activator complex subunit 4-like, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13330
fragaria_vesca	FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830
malus_domestica	MD00G1206400.v1.1 MD00G1206500.v1.1 MD00G1206600.v1.1 MD03G1040600.v1.1 MD11G1042000.v1.1
prunus_persica	Prupe.6G032900_v2.0.a1
pyrus_communis	pycom03g03130 pycom11g03610
rosa_chinensis	RchiOBHm_Chr5g0075021
rosa_laevigata	RLG00000036518
rosa_multiflora	Rmu_sc0006984.1_g000002
rosa_rugosa	Rorug05G0435500
rosa_samantha	Rh5AG492800 Rh5BG514100 Rh5CG537700 Rh5DG526400
rosa_wichuraiana	Rw5G045680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	3029, 3819
AatII	GACGTC	1 cut(s)	1541
Acc16I	TGCGCA	1 cut(s)	2062
Acc36I	ACCTGC	1 cut(s)	960
Acc65I	GGTACC	1 cut(s)	2810
AccB1I	GGYRCC	1 cut(s)	2810
AccI	GTMKAC	1 cut(s)	4378
AccII	CGCG	2 cut(s)	3064, 5340
AccIII	TCCGGA	1 cut(s)	1251
AciI	CCGC	8 cut(s)	248, 559, 2516, 3062, 3064, 4144, 4836, 5207
AclI	AACGTT	1 cut(s)	5393
AcoI	YGGCCR	4 cut(s)	258, 3269, 4070, 4942
AcuI	CTGAAG	7 cut(s)	3000, 4266, 4581, 4806, 5078, 5354, 5643
AcyI	GRCGYC	1 cut(s)	1538
AfeI	AGCGCT	1 cut(s)	4502
AflII	CTTAAG	1 cut(s)	1352
AflIII	ACRYGT	1 cut(s)	5497
AjiI	CACGTC	1 cut(s)	1575
AjuI	GAANNNNNNNTTGG	2 cut(s)	4581, 4613
AloI	GAACNNNNNNTCC	8 cut(s)	2391, 2423, 3828, 3860, 4404, 4405, 4436, 4437
Alw21I	GWGCWC	3 cut(s)	2661, 4667, 5402
Alw26I	GTCTC	7 cut(s)	65, 307, 622, 1493, 1951, 3926, 4898
AlwNI	CAGNNNCTG	4 cut(s)	695, 1418, 1589, 4768
Ama87I	CYCGRG	2 cut(s)	1952, 5401
Aor13HI	TCCGGA	1 cut(s)	1251
Aor51HI	AGCGCT	1 cut(s)	4502
AseI	ATTAAT	1 cut(s)	1715
Asp700I	GAANNNNTTC	4 cut(s)	135, 680, 3222, 3436
Asp718I	GGTACC	1 cut(s)	2810
AspA2I	CCTAGG	1 cut(s)	1312
AspLEI	GCGC	5 cut(s)	2063, 2186, 2301, 3983, 4503
AsuII	TTCGAA	2 cut(s)	129, 3044
AvaI	CYCGRG	2 cut(s)	1952, 5401
AvaII	GGWCC	9 cut(s)	608, 711, 1459, 1919, 2285, 2748, 3155, 3400, 5078
AvrII	CCTAGG	1 cut(s)	1312
BaeGI	GKGCMC	1 cut(s)	3073
BalI	TGGCCA	2 cut(s)	3271, 4072
BanI	GGYRCC	1 cut(s)	2810
BanII	GRGCYC	1 cut(s)	5402
BarI	GAAGNNNNNNTAC	2 cut(s)	2674, 2706
BauI	CACGAG	1 cut(s)	909
BbsI	GAAGAC	4 cut(s)	118, 1734, 2018, 5649
Bbv12I	GWGCWC	3 cut(s)	2661, 4667, 5402
BcgI	CGANNNNNNTGC	2 cut(s)	1521, 1555
BciVI	GTATCC	4 cut(s)	418, 3765, 4294, 4825
BclI	TGATCA	3 cut(s)	3, 2374, 2425
BcoDI	GTCTC	7 cut(s)	65, 307, 622, 1493, 1951, 3926, 4898
BfmI	CTRYAG	4 cut(s)	1590, 2154, 4488, 5283
BfoI	RGCGCY	2 cut(s)	2187, 4504
BfrI	CTTAAG	1 cut(s)	1352
BfuAI	ACCTGC	1 cut(s)	960
BfuI	GTATCC	4 cut(s)	418, 3765, 4294, 4825
BglII	AGATCT	5 cut(s)	722, 1995, 5020, 5144, 5320
BlnI	CCTAGG	1 cut(s)	1312
BlpI	GCTNAGC	1 cut(s)	1132
Bme18I	GGWCC	9 cut(s)	608, 711, 1459, 1919, 2285, 2748, 3155, 3400, 5078
BmeT110I	CYCGRG	2 cut(s)	1952, 5401
BmgBI	CACGTC	1 cut(s)	1575
BmiI	GGNNCC	9 cut(s)	993, 1449, 1851, 2520, 2749, 2750, 2812, 3402, 5079
BmrI	ACTGGG	4 cut(s)	4312, 4404, 4810, 5475
BmuI	ACTGGG	4 cut(s)	4312, 4404, 4810, 5475
BoxI	GACNNNNGTC	1 cut(s)	1106
BpiI	GAAGAC	4 cut(s)	118, 1734, 2018, 5649
BplI	GAGNNNNNCTC	2 cut(s)	275, 307
BpmI	CTGGAG	4 cut(s)	640, 2205, 3537, 5072
Bpu10I	CCTNAGC	2 cut(s)	1118, 5032
Bpu1102I	GCTNAGC	1 cut(s)	1132
Bpu14I	TTCGAA	2 cut(s)	129, 3044
BpuEI	CTTGAG	6 cut(s)	374, 2722, 3368, 4730, 5185, 5654
Bsa29I	ATCGAT	2 cut(s)	1366, 1430
BsaBI	GATNNNNATC	3 cut(s)	1372, 3271, 5319
BsaHI	GRCGYC	1 cut(s)	1538
BsaI	GGTCTC	1 cut(s)	3926
BsaWI	WCCGGW	2 cut(s)	253, 1251
BsaXI	ACNNNNNCTCC	8 cut(s)	269, 299, 2203, 2233, 3828, 3858, 5070, 5100
Bse118I	RCCGGY	3 cut(s)	2973, 3725, 5536
Bse1I	ACTGG	9 cut(s)	1519, 3622, 4318, 4399, 4439, 4816, 5138, 5407, 5481
Bse3DI	GCAATG	3 cut(s)	2693, 3203, 3678
Bse8I	GATNNNNATC	3 cut(s)	1372, 3271, 5319
BseAI	TCCGGA	1 cut(s)	1251
BseCI	ATCGAT	2 cut(s)	1366, 1430
BseJI	GATNNNNATC	3 cut(s)	1372, 3271, 5319
BseMI	GCAATG	3 cut(s)	2693, 3203, 3678
BseMII	CTCAG	7 cut(s)	1146, 1268, 2649, 3602, 3776, 4353, 4845
BseNI	ACTGG	9 cut(s)	1519, 3622, 4318, 4399, 4439, 4816, 5138, 5407, 5481
BseRI	GAGGAG	4 cut(s)	278, 295, 3691, 4214
BseSI	GKGCMC	1 cut(s)	3073
BseYI	CCCAGC	3 cut(s)	1081, 3236, 3830
BsgI	GTGCAG	5 cut(s)	942, 2274, 3378, 3771, 4506
Bsh1236I	CGCG	2 cut(s)	3064, 5340
BshNI	GGYRCC	1 cut(s)	2810
BshVI	ATCGAT	2 cut(s)	1366, 1430
BsiHKAI	GWGCWC	3 cut(s)	2661, 4667, 5402
BsiHKCI	CYCGRG	2 cut(s)	1952, 5401
BsiSI	CCGG	8 cut(s)	254, 673, 1252, 2787, 2974, 3726, 5492, 5537
BslFI	GGGAC	6 cut(s)	869, 1561, 2329, 2761, 4989, 5091
BsmAI	GTCTC	7 cut(s)	65, 307, 622, 1493, 1951, 3926, 4898
BsmBI	CGTCTC	4 cut(s)	65, 307, 1493, 4898
BsmFI	GGGAC	6 cut(s)	869, 1561, 2329, 2761, 4989, 5091
BsmI	GAATGC	4 cut(s)	137, 3423, 4267, 4702
Bso31I	GGTCTC	1 cut(s)	3926
BsoBI	CYCGRG	2 cut(s)	1952, 5401
Bsp119I	TTCGAA	2 cut(s)	129, 3044
Bsp1286I	GDGCHC	4 cut(s)	2661, 3073, 4667, 5402
Bsp13I	TCCGGA	1 cut(s)	1251
Bsp1720I	GCTNAGC	1 cut(s)	1132
Bsp68I	TCGCGA	1 cut(s)	5340
BspACI	CCGC	8 cut(s)	248, 559, 2516, 3062, 3064, 4144, 4836, 5207
BspCNI	CTCAG	7 cut(s)	1145, 1269, 2648, 3601, 3775, 4354, 4846
BspDI	ATCGAT	2 cut(s)	1366, 1430
BspEI	TCCGGA	1 cut(s)	1251
BspFNI	CGCG	2 cut(s)	3064, 5340
BspHI	TCATGA	2 cut(s)	3451, 4696
BspLI	GGNNCC	9 cut(s)	993, 1449, 1851, 2520, 2749, 2750, 2812, 3402, 5079
BspMAI	CTGCAG	2 cut(s)	1594, 5287
BspMI	ACCTGC	1 cut(s)	960
BspQI	GCTCTTC	2 cut(s)	4584, 5619
BspT104I	TTCGAA	2 cut(s)	129, 3044
BspT107I	GGYRCC	1 cut(s)	2810
BspTI	CTTAAG	1 cut(s)	1352
BspTNI	GGTCTC	1 cut(s)	3926
BsrDI	GCAATG	3 cut(s)	2693, 3203, 3678
BsrFI	RCCGGY	3 cut(s)	2973, 3725, 5536
BsrI	ACTGG	9 cut(s)	1519, 3622, 4318, 4399, 4439, 4816, 5138, 5407, 5481
BssAI	RCCGGY	3 cut(s)	2973, 3725, 5536
BssNAI	GTATAC	1 cut(s)	4379
BssNI	GRCGYC	1 cut(s)	1538
BssSI	CACGAG	1 cut(s)	909
BssT1I	CCWWGG	6 cut(s)	411, 1312, 1494, 1698, 2395, 4215
Bst1107I	GTATAC	1 cut(s)	4379
Bst2BI	CACGAG	1 cut(s)	909
Bst6I	CTCTTC	8 cut(s)	189, 2679, 3047, 3421, 4584, 5372, 5373, 5619
BstACI	GRCGYC	1 cut(s)	1538
BstAFI	CTTAAG	1 cut(s)	1352
BstAPI	GCANNNNNTGC	4 cut(s)	214, 223, 3365, 3785
BstBI	TTCGAA	2 cut(s)	129, 3044
BstDSI	CCRYGG	1 cut(s)	3062
BstENI	CCTNNNNNAGG	1 cut(s)	5303
BstFNI	CGCG	2 cut(s)	3064, 5340
BstH2I	RGCGCY	2 cut(s)	2187, 4504
BstHHI	GCGC	5 cut(s)	2063, 2186, 2301, 3983, 4503
BstMAI	GTCTC	7 cut(s)	65, 307, 622, 1493, 1951, 3926, 4898
BstNSI	RCATGY	7 cut(s)	1939, 2067, 2852, 4168, 4609, 5279, 5501
BstPAI	GACNNNNGTC	1 cut(s)	1106
BstSFI	CTRYAG	4 cut(s)	1590, 2154, 4488, 5283
BstSLI	GKGCMC	1 cut(s)	3073
BstUI	CGCG	2 cut(s)	3064, 5340
BstV2I	GAAGAC	4 cut(s)	118, 1734, 2018, 5649
BstX2I	RGATCY	7 cut(s)	722, 1995, 2638, 4089, 5020, 5144, 5320
BstXI	CCANNNNNNTGG	1 cut(s)	618
BstYI	RGATCY	7 cut(s)	722, 1995, 2638, 4089, 5020, 5144, 5320
BstZ17I	GTATAC	1 cut(s)	4379
Bsu15I	ATCGAT	2 cut(s)	1366, 1430
BsuI	GTATCC	4 cut(s)	418, 3765, 4294, 4825
BsuTUI	ATCGAT	2 cut(s)	1366, 1430
BtgI	CCRYGG	1 cut(s)	3062
BtgZI	GCGATG	2 cut(s)	1078, 5517
BtrI	CACGTC	1 cut(s)	1575
BtsI	GCAGTG	3 cut(s)	1539, 1864, 4238
BtsIMutI	CAGTG	6 cut(s)	1539, 1864, 3458, 3797, 4238, 4662
BtuMI	TCGCGA	1 cut(s)	5340
BveI	ACCTGC	1 cut(s)	960
CaiI	CAGNNNCTG	4 cut(s)	695, 1418, 1589, 4768
CciI	TCATGA	2 cut(s)	3451, 4696
CfoI	GCGC	5 cut(s)	2063, 2186, 2301, 3983, 4503
Cfr10I	RCCGGY	3 cut(s)	2973, 3725, 5536
Cfr42I	CCGCGG	1 cut(s)	3065
ClaI	ATCGAT	2 cut(s)	1366, 1430
CseI	GACGC	3 cut(s)	1881, 3280, 4915
CsiI	ACCWGGT	1 cut(s)	688
DraI	TTTAAA	2 cut(s)	3252, 3747
EaeI	YGGCCR	4 cut(s)	258, 3269, 4070, 4942
Eam1104I	CTCTTC	8 cut(s)	189, 2679, 3047, 3421, 4584, 5372, 5373, 5619
EarI	CTCTTC	8 cut(s)	189, 2679, 3047, 3421, 4584, 5372, 5373, 5619
Ecl136II	GAGCTC	1 cut(s)	5400
Eco130I	CCWWGG	6 cut(s)	411, 1312, 1494, 1698, 2395, 4215
Eco147I	AGGCCT	3 cut(s)	1123, 1875, 5298
Eco24I	GRGCYC	1 cut(s)	5402
Eco31I	GGTCTC	1 cut(s)	3926
Eco47I	GGWCC	9 cut(s)	608, 711, 1459, 1919, 2285, 2748, 3155, 3400, 5078
Eco47III	AGCGCT	1 cut(s)	4502
Eco53kI	GAGCTC	1 cut(s)	5400
Eco57I	CTGAAG	7 cut(s)	3000, 4266, 4581, 4806, 5078, 5354, 5643
Eco88I	CYCGRG	2 cut(s)	1952, 5401
EcoICRI	GAGCTC	1 cut(s)	5400
EcoNI	CCTNNNNNAGG	1 cut(s)	5303
EcoO109I	RGGNCCY	3 cut(s)	2285, 2748, 5078
EcoRI	GAATTC	3 cut(s)	1625, 3432, 4864
EcoT14I	CCWWGG	6 cut(s)	411, 1312, 1494, 1698, 2395, 4215
EcoT22I	ATGCAT	4 cut(s)	228, 241, 1216, 1663
EcoT38I	GRGCYC	1 cut(s)	5402
ErhI	CCWWGG	6 cut(s)	411, 1312, 1494, 1698, 2395, 4215
Esp3I	CGTCTC	4 cut(s)	65, 307, 1493, 4898
FaqI	GGGAC	6 cut(s)	869, 1561, 2329, 2761, 4989, 5091
FbaI	TGATCA	3 cut(s)	3, 2374, 2425
FblI	GTMKAC	1 cut(s)	4378
FriOI	GRGCYC	1 cut(s)	5402
FspI	TGCGCA	1 cut(s)	2062
GlaI	GCGC	5 cut(s)	2062, 2185, 2300, 3982, 4502
GsaI	CCCAGC	3 cut(s)	1085, 3240, 3834
GsuI	CTGGAG	4 cut(s)	640, 2205, 3537, 5072
HaeII	RGCGCY	2 cut(s)	2187, 4504
HapII	CCGG	8 cut(s)	254, 673, 1252, 2787, 2974, 3726, 5492, 5537
HgaI	GACGC	3 cut(s)	1881, 3280, 4915
HhaI	GCGC	5 cut(s)	2063, 2186, 2301, 3983, 4503
Hin1I	GRCGYC	1 cut(s)	1538
Hin6I	GCGC	5 cut(s)	2061, 2184, 2299, 3981, 4501
HinP1I	GCGC	5 cut(s)	2061, 2184, 2299, 3981, 4501
HincII	GTYRAC	2 cut(s)	1162, 4279
HindII	GTYRAC	2 cut(s)	1162, 4279
HindIII	AAGCTT	3 cut(s)	473, 4635, 5346
HpaII	CCGG	8 cut(s)	254, 673, 1252, 2787, 2974, 3726, 5492, 5537
Hpy166II	GTNNAC	6 cut(s)	1162, 3022, 3158, 3230, 4279, 4379
Hpy8I	GTNNAC	6 cut(s)	1162, 3022, 3158, 3230, 4279, 4379
Hpy99I	CGWCG	5 cut(s)	335, 1543, 5580, 5656, 5668
HpyCH4IV	ACGT	6 cut(s)	1538, 1574, 4381, 5393, 5578, 5651
HpySE526I	ACGT	6 cut(s)	1538, 1574, 4381, 5393, 5578, 5651
Hsp92I	GRCGYC	1 cut(s)	1538
HspAI	GCGC	5 cut(s)	2061, 2184, 2299, 3981, 4501
KflI	GGGWCCC	1 cut(s)	2748
Kpn2I	TCCGGA	1 cut(s)	1251
KpnI	GGTACC	1 cut(s)	2814
Ksp22I	TGATCA	3 cut(s)	3, 2374, 2425
KspI	CCGCGG	1 cut(s)	3065
LguI	GCTCTTC	2 cut(s)	4584, 5619
LmnI	GCTCC	5 cut(s)	1141, 1447, 2366, 2789, 4575
MabI	ACCWGGT	1 cut(s)	688
MaeII	ACGT	6 cut(s)	1538, 1574, 4381, 5393, 5578, 5651
MfeI	CAATTG	2 cut(s)	868, 2034
MflI	RGATCY	7 cut(s)	722, 1995, 2638, 4089, 5020, 5144, 5320
MhlI	GDGCHC	4 cut(s)	2661, 3073, 4667, 5402
MlsI	TGGCCA	2 cut(s)	3271, 4072
MluNI	TGGCCA	2 cut(s)	3271, 4072
MlyI	GAGTC	9 cut(s)	275, 293, 851, 2351, 2665, 3835, 4361, 4694, 5120
MmeI	TCCRAC	2 cut(s)	2026, 2100
Mox20I	TGGCCA	2 cut(s)	3271, 4072
Mph1103I	ATGCAT	4 cut(s)	228, 241, 1216, 1663
MroI	TCCGGA	1 cut(s)	1251
MroXI	GAANNNNTTC	4 cut(s)	135, 680, 3222, 3436
MscI	TGGCCA	2 cut(s)	3271, 4072
MslI	CAYNNNNRTG	6 cut(s)	506, 1781, 2454, 3213, 3599, 4272
Msp20I	TGGCCA	2 cut(s)	3271, 4072
MspA1I	CMGCKG	3 cut(s)	899, 3064, 4520
MspCI	CTTAAG	1 cut(s)	1352
MspI	CCGG	8 cut(s)	254, 673, 1252, 2787, 2974, 3726, 5492, 5537
MunI	CAATTG	2 cut(s)	868, 2034
Mva1269I	GAATGC	4 cut(s)	137, 3423, 4267, 4702
MvnI	CGCG	2 cut(s)	3064, 5340
NlaIV	GGNNCC	9 cut(s)	993, 1449, 1851, 2520, 2749, 2750, 2812, 3402, 5079
NmeAIII	GCCGAG	2 cut(s)	286, 4970
NmuCI	GTSAC	8 cut(s)	8, 1534, 2668, 3327, 3728, 4106, 4456, 5500
NruI	TCGCGA	1 cut(s)	5340
NsbI	TGCGCA	1 cut(s)	2062
NsiI	ATGCAT	4 cut(s)	228, 241, 1216, 1663
NspI	RCATGY	7 cut(s)	1939, 2067, 2852, 4168, 4609, 5279, 5501
NspV	TTCGAA	2 cut(s)	129, 3044
PaeI	GCATGC	3 cut(s)	1939, 4168, 5279
PaeR7I	CTCGAG	1 cut(s)	5401
PagI	TCATGA	2 cut(s)	3451, 4696
PceI	AGGCCT	3 cut(s)	1123, 1875, 5298
PciI	ACATGT	1 cut(s)	5497
PciSI	GCTCTTC	2 cut(s)	4584, 5619
PcsI	WCGNNNNNNNCGW	3 cut(s)	306, 5399, 5660
PctI	GAATGC	4 cut(s)	137, 3423, 4267, 4702
PdmI	GAANNNNTTC	4 cut(s)	135, 680, 3222, 3436
PfoI	TCCNGGA	2 cut(s)	2641, 5088
PleI	GAGTC	9 cut(s)	274, 292, 851, 2350, 2665, 3835, 4360, 4693, 5120
PpsI	GAGTC	9 cut(s)	274, 292, 851, 2350, 2665, 3835, 4360, 4693, 5120
PpuMI	RGGWCCY	3 cut(s)	2285, 2748, 5078
PscI	ACATGT	1 cut(s)	5497
PshAI	GACNNNNGTC	1 cut(s)	1106
PshBI	ATTAAT	1 cut(s)	1715
PsiI	TTATAA	2 cut(s)	3029, 3819
Psp124BI	GAGCTC	1 cut(s)	5402
Psp1406I	AACGTT	1 cut(s)	5393
Psp5II	RGGWCCY	3 cut(s)	2285, 2748, 5078
PspFI	CCCAGC	3 cut(s)	1081, 3236, 3830
PspN4I	GGNNCC	9 cut(s)	993, 1449, 1851, 2520, 2749, 2750, 2812, 3402, 5079
PspPPI	RGGWCCY	3 cut(s)	2285, 2748, 5078
PspXI	VCTCGAGB	1 cut(s)	5401
PsrI	GAACNNNNNNTAC	2 cut(s)	2137, 2169
PstI	CTGCAG	2 cut(s)	1594, 5287
PstNI	CAGNNNCTG	4 cut(s)	695, 1418, 1589, 4768
PsuI	RGATCY	7 cut(s)	722, 1995, 2638, 4089, 5020, 5144, 5320
PvuII	CAGCTG	2 cut(s)	899, 4520
RruI	TCGCGA	1 cut(s)	5340
RseI	CAYNNNNRTG	6 cut(s)	506, 1781, 2454, 3213, 3599, 4272
SacI	GAGCTC	1 cut(s)	5402
SacII	CCGCGG	1 cut(s)	3065
SapI	GCTCTTC	2 cut(s)	4584, 5619
SchI	GAGTC	9 cut(s)	275, 293, 851, 2351, 2665, 3835, 4361, 4694, 5120
SduI	GDGCHC	4 cut(s)	2661, 3073, 4667, 5402
SexAI	ACCWGGT	1 cut(s)	688
SfcI	CTRYAG	4 cut(s)	1590, 2154, 4488, 5283
Sfr274I	CTCGAG	1 cut(s)	5401
Sfr303I	CCGCGG	1 cut(s)	3065
SfuI	TTCGAA	2 cut(s)	129, 3044
SgrAI	CRCCGGYG	1 cut(s)	5536
SgrBI	CCGCGG	1 cut(s)	3065
SinI	GGWCC	9 cut(s)	608, 711, 1459, 1919, 2285, 2748, 3155, 3400, 5078
SlaI	CTCGAG	1 cut(s)	5401
SmiMI	CAYNNNNRTG	6 cut(s)	506, 1781, 2454, 3213, 3599, 4272
SmlI	CTYRAG	8 cut(s)	389, 1352, 2737, 3383, 4709, 5200, 5401, 5633
SmoI	CTYRAG	8 cut(s)	389, 1352, 2737, 3383, 4709, 5200, 5401, 5633
SphI	GCATGC	3 cut(s)	1939, 4168, 5279
SseBI	AGGCCT	3 cut(s)	1123, 1875, 5298
SsiI	CCGC	8 cut(s)	248, 559, 2516, 3062, 3064, 4144, 4836, 5207
SspI	AATATT	7 cut(s)	1178, 1759, 2020, 3906, 4263, 4846, 5602
SstI	GAGCTC	1 cut(s)	5402
StuI	AGGCCT	3 cut(s)	1123, 1875, 5298
StyI	CCWWGG	6 cut(s)	411, 1312, 1494, 1698, 2395, 4215
TaiI	ACGT	6 cut(s)	1541, 1577, 4384, 5396, 5581, 5654
TaqII	GACCGA	1 cut(s)	654
TatI	WGTACW	4 cut(s)	2430, 3098, 4564, 5434
TauI	GCSGC	5 cut(s)	250, 562, 2519, 3064, 4147
TscAI	CASTG	6 cut(s)	1539, 1864, 3465, 3804, 4238, 4662
TseFI	GTSAC	8 cut(s)	8, 1534, 2668, 3327, 3728, 4106, 4456, 5500
Tsp45I	GTSAC	8 cut(s)	8, 1534, 2668, 3327, 3728, 4106, 4456, 5500
TspGWI	ACGGA	4 cut(s)	47, 298, 334, 3331
TspRI	CASTG	6 cut(s)	1539, 1864, 3465, 3804, 4238, 4662
Vha464I	CTTAAG	1 cut(s)	1352
VpaK11BI	GGWCC	9 cut(s)	608, 711, 1459, 1919, 2285, 2748, 3155, 3400, 5078
VspI	ATTAAT	1 cut(s)	1715
XagI	CCTNNNNNAGG	1 cut(s)	5303
XbaI	TCTAGA	1 cut(s)	3524
XceI	RCATGY	7 cut(s)	1939, 2067, 2852, 4168, 4609, 5279, 5501
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1190
XhoI	CTCGAG	1 cut(s)	5401
XmaJI	CCTAGG	1 cut(s)	1312
XmiI	GTMKAC	1 cut(s)	4378
XmnI	GAANNNNTTC	4 cut(s)	135, 680, 3222, 3436
ZraI	GACGTC	1 cut(s)	1539
Zsp2I	ATGCAT	4 cut(s)	228, 241, 1216, 1663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.