Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0005110

Physical Location & Seq

Genomic Coordinates Forward (+)

5358747 .. 5369786

Gene Structure 11040 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4062 bp

ATGGATCCCATAGAAGCCGCGAACGGGCTCTCGTTCCGGGTCGGATTCTCTGGCCACAGTGGCCACCTCCGGCTTGAGCCCCTCTCCACCGTTGAAAGCTCCGACCCCGTCAAGTCTCTTCCTGATTTCATTCTGCCGCCTGCTTTTCCTAGGGAGACGCCCGGATCGATAAAAGAGTATATAGAGGAGAAGTATCTGTCGCTGAGATTGGATGACGAAGTATTTGCACCCGAAAAGGTTGGGAGGCAGTGGGATTTTGACTGGTTTGATAAGGCAGATGTTCCCTTGGAGCCATCACTGCCGCGCTGCGTTGTGGTTCCCACGTGGGAATTGCCATTTCGGCGCCAGAAGAATGGGTCGGCTGAAGGTGAATGGGAACCTAAATCTGTGCAGGTGGATGTGTCAGAACTTATAGTAGGAGCTCGAGAGTCTGGTTCATTGCCACGTTTGGCTGGACCAGCTAAGGATTTTGTACGGGGAAGCATCAACAACCGCCCTTTTCGTCCAGGAGGCTTGGATGATTCCCAATCTCTAGAAAGAGTTGTTCCTGATGGTGCTTGTAATGGCGAATGGGTACGCGAACTTCTGGTTGGTGGCTCTCCTCAGGCTGTACCTCCAAGCTTTAAGCAAGGATTGGACCTTGGTGATCTGAAGGCCTATCCATGCTCGTGGAATGTATATAAGGATCAAAGTTCCATGGAGAGCACGTCAGATGAAAAACTGTATTCGCAGAGTGGGTTGTCTGTACAGTTTGATGACCTGTTCAAGAAGGCTTGGGAAGAGGAAGTTGTTGAATTTGAAGGAGATGGTCAATTGTCAGGATCGGAATCTGTTAAGTCAGAGGATGAAGCAAAGGAGGTTGATGTTGCCATCGATTCTTGTGAGCCTGAATTGTCTTTGTTGGATGAGCTTTTGTCAGTTGAGGCAGGAGAAACAAAGTCAAGATCTAATGGAACTGGTGAAGATGATGAACAAAATCCGAAAGTGGCTTGGGCTATTAGTGGAGGTACTGAATATATTGCACAGAACTTTCATGATCTTGTTCCCAACAAAGCAATGGATTTTCCTTTTGAATTGGACAAATTCCAGAAGGAGGCTATTTATTATCTTGAAAAGGGGGACTCGGTATTTGTGGCTGCTCACACATCAGCTGGAAAGACAGTTGTTGCAGAATATGCATTTGCTTTAGCATCAAAACATTGCACCAGAGCTGTGTATACAGCTCCTATCAAAACCATCAGCAACCAAAAATATAGAGATTTGTGTGGAAAATTTGATGTTGGACTTCTCACTGGTGATGTTAGCTTGAGGCCAGAGGCTTCTTGTCTCATTATGACAACCGAAATATTGAGGTCAATGCTTTATCGCGGTGCTGACATAATACGTGATATTGAATGGGTTATCTTTGATGAAGTGCACTACGTCAATGATGTCGAAAGAGGTGTTGTTTGGGAAGAAGTTATTATAATGCTTCCAAGACACATCAATATTGTCCTCCTTTCAGCTACGGTACCAAACAAAGTCGAGTTTGCTGACTGGATTGGACGGACAAAGCAAAAGCAAATCCGTGTTACCGGGACTACAAAAAGACCAGTCCCATTGGAGCACTGTCTATTTTACTCCGGAGAACTTTACAAAATATGTGAAAGTGAAAGCTTTATTCCCAAGGGGTTCGAAGCTGCAAAAGATGCATTTAAGAAAAAGAATATGAGTTCTGCCACTGGTGGTAGTGGATCACAGGCACCAGCTTCAGCTTCTCATGATGGGACTCGATCTCAAAAACAAAGTTCAAACTGGGGCGGAAAACAGAAGAAGCAATCTGGTCCCCAAAATTCAGGGAATTTCTCCCGAACGAGAGGAGAAAATCAAAACAAAAATGGCATGAACAGTTGGGGTTTAAGAAGATCAGATGTTTCTTTGTGGTTGTCACTTATTAACAAGCTCTCAAAGATGTCACTATTACCTGTGGTGGTATTTTGTTTCTCAAAGAATCGCTGTGATAAGTCAGTTGATAGTATGTATGGGACTGACCTTACAAGTAGTTCTGAGAAAAGTGAGATTCGTGTTTTCTGTGACAAAGCATTTTCGCGGCTAAAGGGATCTGATAGGACTTTACCACAGGTTGTCAGAGTTCAAAGCCTTCTTCATAGAGGGATTGGTGTCCATCATGCTGGACTGCTTCCAATTGTTAAGGAAGTTGTGGAAATGCTTTTTTGTCGTGGAGTGATCAAGGTTTTGTTCTCAACAGAGACATTTGCAATGGGGGTTAATGCACCAGCTAGAACGGTTGTTTTTGATACATTAAGGAAATTTGATGGCAAGGAATTTAGACAATTACTTCCAGGAGAATACACTCAAATGGCAGGGCGTGCTGGCAGAAGAGGACTTGATAAAATTGGTACAGTTATTGTAATGTGCCGTGATGAAATCCTAGGAGAGAGTGATTTGAAGCATGTCATAGTTGGAAGTGCAACCAGGCTTGAATCTCAGTTTCGGCTTACCTATATTATGATCCTGCATCTCCTTCGTGTTGAGGAACTGAAGGTGGAGGACATGCTGAAAAGAAGTTTTGCTGAGTTCCATGCTCAGAAGAAACTACCAGAACAGCAGCAACTTCTGATGCGAAAGCTTGCTCAGGCTACAAAAACTATCGAATGTATAAAAGGTGAACCAGCTATTGAGGAGTATTATGACATCCACTCGGAAGCTGAGAATCATAACACGGAAATATTGGAGGCAGTTATGCAGTCTCCTGTTGCCCAACAATTTCTTACAACTGGGAGAGTGGTAGTCATGAAATCACAGTCAGAGCGGGACCACTTGCTTGGAGTCGTCGTGAAAGCACCTTGTGCTACTAATAAACAATATATTGTTTTGGTGCTGAAACATGAACCACCGCCGCAGACTCCACTGGGTAGTGGTAACTTGCAAGATAGCAAAAGCACGGACTTTCGACAAGGGTTTTTTGTGGCACCAAAATCTAAACGTGGTCTTGATGAAGATTATTGTTCTGGTGTCAAAACTCGGAAAGGAAAGGGTGTCATCAACATAATATTACCACACCAGGGTTCTGCTGCTGGGGTGAGCTTTGAGGTTAAAGGAGTCGACATCAAAGATTTTCTATGCATATGCAATTGCAAGATAAAAATTGACCAAGTTGGGCTTCTTGAAGACAATAGCAATGCTGCTTACTCCAAGACAGTTCAACAGCTGTTGGACACAAAATCCGATGAAAATAAGTACCCTCCAGCCTTGGATCCACGTAAAGATCTGAAGTTGACAGATGTGAATCTCGTGGCAACATACTACAAGTGGACTAACTTATTGCAAAAGATGGCAAAGAATAAGTGCCATGGATGTATAAAGTTGGACGAGCACAGTAAGTTAGCAAGAGAGAAAAAGAGACACAGTGAGGAAGTCGATGCTCTTAAGTTTGAAATGTCAGATGAAGCACTTCAACAAATGCCAGACTTTCAAGGCCGGATAGATGTTCTGCAGGAAATTGGATGTATCGATGCTGATCTTGTGGTTCAAATAAAAGGCCGTGTTGCTTGCGAAATGAACTCTGGGGAGGAGTTGATAGCCACAGAGTGTTTGTTTGAAAACCAACTGGATGACCTGGAACCAGAAGAAGCGGTGGCGTTGATGTCTGCTTTTGTGTTTCAGCAGAAGAATACTTCTGAACCTTCTCTTACTCCAAAACTGTCTCAGGCCAAACAGAGGTTGTACGATACAGCAATAAGACTTGGCCAGCTTCAATCCCACTTCAAACTTCAAATAAATCCAGAGGAGTACGCTCGAGAGAATCTCAAGTTTGGTCTTGTTGAAGTCGTTTATGAGTGGGCGAAGGGCACTCCGTTTGCAGACATTTGTGAACTTACAGATGTTGCTGAAGGAATGATAGTGCGAACCATTGTCAGACTTGATGAGACGTGTCGTGAGTTCAAAAATGCTGCATCGATTATGGGTAATTCTGCTCTGTACAAGAAAATGGTAACAGCTTCAAATGCAATAAAGCGCGACATTGTATTTGCAGCTAGCTTATACGTCACCGGAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0015672 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0030001 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0035864 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055087 GO:0060255 GO:0065007 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1354

Amino Acids

150.95

Weight (kDa)

5.71

Isoelectric Point (pI)

43.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ski2_N	PF17911	141 - 215	9.8e-07	Ski2 N-terminal region
DEAD	PF00270	361 - 509	9.3e-23	DEAD/DEAH box helicase
Helicase_C	PF00271	716 - 795	1e-05	Helicase conserved C-terminal domain
MTR4-like_stalk	PF21408	850 - 914	4e-13	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	917 - 1149	6.6e-28	Mtr4-like, beta-barrel domain
DSHCT	PF08148	1175 - 1345	5.8e-59	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46960 AT3G46960 AT3G46960
fragaria_vesca	FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880
malus_domestica	MD03G1077900.v1.1 MD11G1082500.v1.1
prunus_persica	Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1
pyrus_communis	pycom03g06210 pycom11g07060
rosa_chinensis	RchiOBHm_Chr5g0068091
rosa_laevigata	RLG00000035954
rosa_multiflora	Rmu_sc0000435.1_g000017
rosa_roxburghii	Rroxscaffold_1G00012520 Rroxscaffold_1G00013260
rosa_rugosa	Rorug05G0387900 Rorug05G0388300 Rorug05G0388400 Rorug05G0388500
rosa_samantha	Rh5AG446500 Rh5BG464600 Rh5CG486400 Rh5DG478400
rosa_wichuraiana	Rw5G041700 Rw5G041740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1466
AarI	CACCTGC	1 cut(s)	382
Acc36I	ACCTGC	1 cut(s)	382
Acc65I	GGTACC	1 cut(s)	1510
AccB1I	GGYRCC	4 cut(s)	342, 1510, 1741, 2971
AccBSI	CCGCTC	1 cut(s)	2812
AccI	GTMKAC	2 cut(s)	1217, 3105
AccII	CGCG	6 cut(s)	20, 304, 579, 1368, 2089, 4020
AccIII	TCCGGA	1 cut(s)	1622
AclWI	GGATC	9 cut(s)	12, 172, 693, 829, 1741, 2107, 2506, 3251, 3264
AcoI	YGGCCR	3 cut(s)	52, 61, 3748
AcsI	RAATTY	7 cut(s)	794, 1082, 1271, 1831, 1840, 2309, 2324
AcuI	CTGAAG	6 cut(s)	384, 671, 1734, 2561, 3293, 3912
AcvI	CACGTG	1 cut(s)	324
AcyI	GRCGYC	2 cut(s)	158, 343
AdeI	CACNNNGTG	2 cut(s)	2849, 3591
AfiI	CCNNNNNNNGG	4 cut(s)	24, 1093, 3065, 3720
AflII	CTTAAG	1 cut(s)	3428
AflIII	ACRYGT	1 cut(s)	3932
AjiI	CACGTC	2 cut(s)	708, 3933
AjnI	CCWGG	5 cut(s)	505, 2341, 2474, 3063, 3618
AjuI	GAANNNNNNNTTGG	4 cut(s)	573, 605, 3147, 3179
Alw21I	GWGCWC	5 cut(s)	424, 707, 1419, 1608, 3378
Alw26I	GTCTC	8 cut(s)	120, 149, 1331, 2243, 2754, 3397, 3711, 3923
Alw44I	GTGCAC	1 cut(s)	1415
AlwI	GGATC	9 cut(s)	12, 172, 693, 829, 1741, 2107, 2506, 3251, 3264
Ama87I	CYCGRG	2 cut(s)	423, 3798
Aor13HI	TCCGGA	1 cut(s)	1622
AoxI	GGCC	8 cut(s)	52, 61, 654, 1310, 3478, 3541, 3711, 3748
ApaLI	GTGCAC	1 cut(s)	1415
ApeKI	GCWGC	8 cut(s)	306, 1136, 1679, 2608, 3074, 3185, 3953, 4034
ApoI	RAATTY	7 cut(s)	794, 1082, 1271, 1831, 1840, 2309, 2324
ArsI	GACNNNNNNTTYG	6 cut(s)	206, 238, 1112, 1144, 4013, 4045
Asp700I	GAANNNNTTC	1 cut(s)	3453
Asp718I	GGTACC	1 cut(s)	1510
AspA2I	CCTAGG	2 cut(s)	149, 2431
AspLEI	GCGC	3 cut(s)	306, 345, 4020
AspS9I	GGNCC	4 cut(s)	455, 637, 1823, 2815
AsuC2I	CCSGG	3 cut(s)	38, 162, 1576
AsuHPI	GGTGA	7 cut(s)	380, 656, 971, 1307, 2678, 3094, 4042
AsuII	TTCGAA	1 cut(s)	1674
AsuNHI	GCTAGC	1 cut(s)	4037
AvaI	CYCGRG	2 cut(s)	423, 3798
AvaII	GGWCC	4 cut(s)	455, 637, 1823, 2815
AvrII	CCTAGG	2 cut(s)	149, 2431
AxyI	CCTNAGG	1 cut(s)	603
BaeGI	GKGCMC	2 cut(s)	1419, 3854
BalI	TGGCCA	3 cut(s)	54, 63, 3750
BamHI	GGATCC	2 cut(s)	4, 3256
BanI	GGYRCC	4 cut(s)	342, 1510, 1741, 2971
BanII	GRGCYC	3 cut(s)	30, 81, 424
BarI	GAAGNNNNNNTAC	2 cut(s)	567, 599
BauI	CACGAG	2 cut(s)	667, 3293
BbrPI	CACGTG	1 cut(s)	324
BbsI	GAAGAC	1 cut(s)	3177
Bbv12I	GWGCWC	5 cut(s)	424, 707, 1419, 1608, 3378
BbvI	GCAGC	8 cut(s)	293, 1123, 1666, 2620, 3061, 3172, 3940, 4046
BccI	CCATC	9 cut(s)	301, 545, 800, 878, 1244, 1757, 2172, 2309, 3328
BceAI	ACGGC	2 cut(s)	2403, 3528
BcgI	CGANNNNNNTGC	4 cut(s)	206, 240, 2507, 2541
BciT130I	CCWGG	5 cut(s)	507, 2343, 2476, 3065, 3620
BclI	TGATCA	1 cut(s)	2226
BcnI	CCSGG	3 cut(s)	38, 162, 1576
BcoDI	GTCTC	8 cut(s)	120, 149, 1331, 2243, 2754, 3397, 3711, 3923
BfaI	CTAG	5 cut(s)	150, 533, 2280, 2432, 4038
BfmI	CTRYAG	1 cut(s)	3494
BfoI	RGCGCY	1 cut(s)	346
BfrI	CTTAAG	1 cut(s)	3428
BfuAI	ACCTGC	1 cut(s)	382
BglI	GCCNNNNNGGC	2 cut(s)	60, 340
BglII	AGATCT	2 cut(s)	944, 3268
BlnI	CCTAGG	2 cut(s)	149, 2431
Bme1390I	CCNGG	8 cut(s)	38, 162, 507, 1576, 2343, 2476, 3065, 3620
Bme18I	GGWCC	4 cut(s)	455, 637, 1823, 2815
BmeT110I	CYCGRG	2 cut(s)	423, 3798
BmgBI	CACGTC	2 cut(s)	708, 3933
BmgT120I	GGNCC	4 cut(s)	455, 637, 1823, 2815
BmrFI	CCNGG	8 cut(s)	38, 162, 507, 1576, 2343, 2476, 3065, 3620
BmrI	ACTGGG	3 cut(s)	1804, 2787, 2921
BmsI	GCATC	8 cut(s)	492, 1199, 1678, 2527, 2610, 3412, 3505, 3965
BmtI	GCTAGC	1 cut(s)	4041
BmuI	ACTGGG	3 cut(s)	1804, 2787, 2921
BpiI	GAAGAC	1 cut(s)	3177
BplI	GAGNNNNNCTC	4 cut(s)	68, 100, 3792, 3824
BpmI	CTGGAG	1 cut(s)	3231
Bpu10I	CCTNAGC	2 cut(s)	462, 2634
Bpu14I	TTCGAA	1 cut(s)	1674
BpuEI	CTTGAG	3 cut(s)	95, 1327, 3794
BpuMI	CCSGG	3 cut(s)	38, 162, 1576
Bsa29I	ATCGAT	4 cut(s)	167, 873, 3513, 3959
BsaAI	YACGTR	3 cut(s)	324, 1385, 3263
BsaBI	GATNNNNATC	3 cut(s)	826, 3288, 3519
BsaHI	GRCGYC	2 cut(s)	158, 343
BsaJI	CCNNGG	9 cut(s)	149, 285, 640, 696, 1665, 2431, 3064, 3252, 3352
BsaWI	WCCGGW	2 cut(s)	1622, 4052
BsaXI	ACNNNNNCTCC	4 cut(s)	692, 722, 2820, 2850
Bsc4I	CCNNNNNNNGG	4 cut(s)	24, 1093, 3065, 3720
Bse21I	CCTNAGG	1 cut(s)	603
Bse3DI	GCAATG	5 cut(s)	437, 1062, 1198, 2265, 3187
Bse8I	GATNNNNATC	3 cut(s)	826, 3288, 3519
BseAI	TCCGGA	1 cut(s)	1622
BseBI	CCWGG	5 cut(s)	507, 2343, 2476, 3065, 3620
BseCI	ATCGAT	4 cut(s)	167, 873, 3513, 3959
BseDI	CCNNGG	9 cut(s)	149, 285, 640, 696, 1665, 2431, 3064, 3252, 3352
BseGI	GGATG	9 cut(s)	217, 403, 523, 850, 910, 2694, 3362, 3512, 3619
BseJI	GATNNNNATC	3 cut(s)	826, 3288, 3519
BseLI	CCNNNNNNNGG	4 cut(s)	24, 1093, 3065, 3720
BseMI	GCAATG	5 cut(s)	437, 1062, 1198, 2265, 3187
BseMII	CTCAG	9 cut(s)	194, 617, 2037, 2501, 2565, 2600, 2648, 2700, 3722
BseRI	GAGGAG	6 cut(s)	200, 591, 1872, 2696, 3587, 3803
BseSI	GKGCMC	2 cut(s)	1419, 3854
BseXI	GCAGC	8 cut(s)	293, 1123, 1666, 2620, 3061, 3172, 3940, 4046
BseYI	CCCAGC	1 cut(s)	3077
BsgI	GTGCAG	1 cut(s)	410
Bsh1236I	CGCG	6 cut(s)	20, 304, 579, 1368, 2089, 4020
BshFI	GGCC	8 cut(s)	54, 63, 656, 1312, 3480, 3543, 3713, 3750
BshNI	GGYRCC	4 cut(s)	342, 1510, 1741, 2971
BshVI	ATCGAT	4 cut(s)	167, 873, 3513, 3959
BsiHKAI	GWGCWC	5 cut(s)	424, 707, 1419, 1608, 3378
BsiHKCI	CYCGRG	2 cut(s)	423, 3798
BsiSI	CCGG	7 cut(s)	37, 70, 162, 1575, 1623, 3481, 4053
BslFI	GGGAC	7 cut(s)	1133, 1580, 1591, 1780, 1809, 2038, 2828
BslI	CCNNNNNNNGG	4 cut(s)	24, 1093, 3065, 3720
BsmAI	GTCTC	8 cut(s)	120, 149, 1331, 2243, 2754, 3397, 3711, 3923
BsmBI	CGTCTC	2 cut(s)	149, 3923
BsmFI	GGGAC	7 cut(s)	1133, 1580, 1591, 1780, 1809, 2038, 2828
BsnI	GGCC	8 cut(s)	54, 63, 656, 1312, 3480, 3543, 3713, 3750
BsoBI	CYCGRG	2 cut(s)	423, 3798
Bsp119I	TTCGAA	1 cut(s)	1674
Bsp1286I	GDGCHC	8 cut(s)	30, 81, 424, 707, 1419, 1608, 3378, 3854
Bsp13I	TCCGGA	1 cut(s)	1622
Bsp1407I	TGTACA	2 cut(s)	745, 3981
Bsp19I	CCATGG	2 cut(s)	696, 3352
BspANI	GGCC	8 cut(s)	54, 63, 656, 1312, 3480, 3543, 3713, 3750
BspCNI	CTCAG	9 cut(s)	195, 616, 2038, 2500, 2566, 2599, 2647, 2701, 3721
BspDI	ATCGAT	4 cut(s)	167, 873, 3513, 3959
BspEI	TCCGGA	1 cut(s)	1622
BspFNI	CGCG	6 cut(s)	20, 304, 579, 1368, 2089, 4020
BspHI	TCATGA	3 cut(s)	1033, 1759, 2793
BspMAI	CTGCAG	1 cut(s)	3498
BspMI	ACCTGC	1 cut(s)	382
BspOI	GCTAGC	1 cut(s)	4041
BspPI	GGATC	9 cut(s)	12, 172, 693, 829, 1741, 2107, 2506, 3251, 3264
BspT104I	TTCGAA	1 cut(s)	1674
BspT107I	GGYRCC	4 cut(s)	342, 1510, 1741, 2971
BspTI	CTTAAG	1 cut(s)	3428
BsrBI	CCGCTC	1 cut(s)	2812
BsrDI	GCAATG	5 cut(s)	437, 1062, 1198, 2265, 3187
BsrGI	TGTACA	2 cut(s)	745, 3981
BssECI	CCNNGG	9 cut(s)	149, 285, 640, 696, 1665, 2431, 3064, 3252, 3352
BssNAI	GTATAC	1 cut(s)	1218
BssNI	GRCGYC	2 cut(s)	158, 343
BssSI	CACGAG	2 cut(s)	667, 3293
BssT1I	CCWWGG	8 cut(s)	149, 285, 640, 696, 1665, 2431, 3252, 3352
Bst1107I	GTATAC	1 cut(s)	1218
Bst2BI	CACGAG	2 cut(s)	667, 3293
Bst2UI	CCWGG	5 cut(s)	507, 2343, 2476, 3065, 3620
Bst6I	CTCTTC	3 cut(s)	123, 774, 2374
BstACI	GRCGYC	2 cut(s)	158, 343
BstAFI	CTTAAG	1 cut(s)	3428
BstAPI	GCANNNNNTGC	4 cut(s)	1175, 1688, 2369, 2849
BstAUI	TGTACA	2 cut(s)	745, 3981
BstBAI	YACGTR	3 cut(s)	324, 1385, 3263
BstBI	TTCGAA	1 cut(s)	1674
BstC8I	GCNNGC	7 cut(s)	141, 2370, 2374, 2631, 3553, 3752, 4039
BstDSI	CCRYGG	2 cut(s)	696, 3352
BstF5I	GGATG	9 cut(s)	217, 403, 523, 850, 910, 2694, 3362, 3512, 3619
BstFNI	CGCG	6 cut(s)	20, 304, 579, 1368, 2089, 4020
BstH2I	RGCGCY	1 cut(s)	346
BstHHI	GCGC	3 cut(s)	306, 345, 4020
BstMAI	GTCTC	8 cut(s)	120, 149, 1331, 2243, 2754, 3397, 3711, 3923
BstMWI	GCNNNNNNNGC	9 cut(s)	60, 298, 340, 458, 1175, 1688, 2369, 2849, 4007
BstNI	CCWGG	5 cut(s)	507, 2343, 2476, 3065, 3620
BstNSI	RCATGY	2 cut(s)	2456, 2557
BstSCI	CCNGG	8 cut(s)	36, 160, 505, 1574, 2341, 2474, 3063, 3618
BstSFI	CTRYAG	1 cut(s)	3494
BstSLI	GKGCMC	2 cut(s)	1419, 3854
BstUI	CGCG	6 cut(s)	20, 304, 579, 1368, 2089, 4020
BstV1I	GCAGC	8 cut(s)	293, 1123, 1666, 2620, 3061, 3172, 3940, 4046
BstV2I	GAAGAC	1 cut(s)	3177
BstX2I	RGATCY	5 cut(s)	4, 944, 2099, 3256, 3268
BstXI	CCANNNNNNTGG	4 cut(s)	353, 669, 2171, 2825
BstYI	RGATCY	5 cut(s)	4, 944, 2099, 3256, 3268
BstZ17I	GTATAC	1 cut(s)	1218
Bsu15I	ATCGAT	4 cut(s)	167, 873, 3513, 3959
Bsu36I	CCTNAGG	1 cut(s)	603
BsuRI	GGCC	8 cut(s)	54, 63, 656, 1312, 3480, 3543, 3713, 3750
BsuTUI	ATCGAT	4 cut(s)	167, 873, 3513, 3959
BtgI	CCRYGG	2 cut(s)	696, 3352
BtrI	CACGTC	2 cut(s)	708, 3933
BtsCI	GGATG	9 cut(s)	217, 403, 523, 850, 910, 2694, 3362, 3512, 3619
BtsI	GCAGTG	2 cut(s)	254, 296
BtsIMutI	CAGTG	8 cut(s)	64, 254, 296, 1290, 1606, 1719, 2909, 3415
BveI	ACCTGC	1 cut(s)	382
Cac8I	GCNNGC	7 cut(s)	141, 2370, 2374, 2631, 3553, 3752, 4039
CciI	TCATGA	3 cut(s)	1033, 1759, 2793
CfoI	GCGC	3 cut(s)	306, 345, 4020
Cfr13I	GGNCC	4 cut(s)	455, 637, 1823, 2815
ClaI	ATCGAT	4 cut(s)	167, 873, 3513, 3959
CseI	GACGC	1 cut(s)	166
DinI	GGCGCC	1 cut(s)	344
DraIII	CACNNNGTG	2 cut(s)	2849, 3591
EaeI	YGGCCR	3 cut(s)	52, 61, 3748
Eam1104I	CTCTTC	3 cut(s)	123, 774, 2374
EarI	CTCTTC	3 cut(s)	123, 774, 2374
EciI	GGCGGA	1 cut(s)	1815
Ecl136II	GAGCTC	1 cut(s)	422
Eco130I	CCWWGG	8 cut(s)	149, 285, 640, 696, 1665, 2431, 3252, 3352
Eco147I	AGGCCT	1 cut(s)	656
Eco24I	GRGCYC	3 cut(s)	30, 81, 424
Eco47I	GGWCC	4 cut(s)	455, 637, 1823, 2815
Eco53kI	GAGCTC	1 cut(s)	422
Eco57I	CTGAAG	6 cut(s)	384, 671, 1734, 2561, 3293, 3912
Eco72I	CACGTG	1 cut(s)	324
Eco81I	CCTNAGG	1 cut(s)	603
Eco88I	CYCGRG	2 cut(s)	423, 3798
EcoICRI	GAGCTC	1 cut(s)	422
EcoRII	CCWGG	5 cut(s)	505, 2341, 2474, 3063, 3618
EcoT14I	CCWWGG	8 cut(s)	149, 285, 640, 696, 1665, 2431, 3252, 3352
EcoT22I	ATGCAT	3 cut(s)	1180, 1693, 3128
EcoT38I	GRGCYC	3 cut(s)	30, 81, 424
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	8 cut(s)	149, 285, 640, 696, 1665, 2431, 3252, 3352
Esp3I	CGTCTC	2 cut(s)	149, 3923
FalI	AAGNNNNNCTT	2 cut(s)	3147, 3179
FaqI	GGGAC	7 cut(s)	1133, 1580, 1591, 1780, 1809, 2038, 2828
FauI	CCCGC	1 cut(s)	2805
FauNDI	CATATG	1 cut(s)	3128
FbaI	TGATCA	1 cut(s)	2226
FblI	GTMKAC	2 cut(s)	1217, 3105
FokI	GGATG	9 cut(s)	224, 410, 530, 857, 917, 2681, 3369, 3519, 3626
FriOI	GRGCYC	3 cut(s)	30, 81, 424
FspBI	CTAG	5 cut(s)	150, 533, 2280, 2432, 4038
GlaI	GCGC	3 cut(s)	305, 344, 4019
GsaI	CCCAGC	1 cut(s)	3081
GsuI	CTGGAG	1 cut(s)	3231
HaeII	RGCGCY	1 cut(s)	346
HaeIII	GGCC	8 cut(s)	54, 63, 656, 1312, 3480, 3543, 3713, 3750
HapII	CCGG	7 cut(s)	37, 70, 162, 1575, 1623, 3481, 4053
HgaI	GACGC	1 cut(s)	166
HhaI	GCGC	3 cut(s)	306, 345, 4020
Hin1I	GRCGYC	2 cut(s)	158, 343
Hin6I	GCGC	3 cut(s)	304, 343, 4018
HinP1I	GCGC	3 cut(s)	304, 343, 4018
HincII	GTYRAC	2 cut(s)	3106, 3279
HindII	GTYRAC	2 cut(s)	3106, 3279
HindIII	AAGCTT	3 cut(s)	619, 1654, 2627
HpaII	CCGG	7 cut(s)	37, 70, 162, 1575, 1623, 3481, 4053
HphI	GGTGA	7 cut(s)	380, 656, 971, 1307, 2678, 3094, 4042
Hpy166II	GTNNAC	7 cut(s)	1218, 1417, 2669, 3106, 3279, 3315, 3875
Hpy8I	GTNNAC	7 cut(s)	1218, 1417, 2669, 3106, 3279, 3315, 3875
Hpy99I	CGWCG	1 cut(s)	2837
HpyCH4IV	ACGT	9 cut(s)	323, 445, 707, 1384, 1422, 2986, 3262, 3932, 4047
HpyF10VI	GCNNNNNNNGC	9 cut(s)	60, 298, 340, 458, 1175, 1688, 2369, 2849, 4007
HpySE526I	ACGT	9 cut(s)	323, 445, 707, 1384, 1422, 2986, 3262, 3932, 4047
Hsp92I	GRCGYC	2 cut(s)	158, 343
HspAI	GCGC	3 cut(s)	304, 343, 4018
KasI	GGCGCC	1 cut(s)	342
Kpn2I	TCCGGA	1 cut(s)	1622
KpnI	GGTACC	1 cut(s)	1514
Ksp22I	TGATCA	1 cut(s)	2226
LmnI	GCTCC	5 cut(s)	104, 289, 419, 1228, 1603
Lsp1109I	GCAGC	8 cut(s)	293, 1123, 1666, 2620, 3061, 3172, 3940, 4046
LweI	GCATC	8 cut(s)	492, 1199, 1678, 2527, 2610, 3412, 3505, 3965
MaeI	CTAG	5 cut(s)	150, 533, 2280, 2432, 4038
MaeII	ACGT	9 cut(s)	323, 445, 707, 1384, 1422, 2986, 3262, 3932, 4047
MaeIII	GTNAC	7 cut(s)	1570, 1926, 1953, 2072, 2921, 3994, 4048
MbiI	CCGCTC	1 cut(s)	2812
MfeI	CAATTG	3 cut(s)	812, 2184, 3133
MflI	RGATCY	5 cut(s)	4, 944, 2099, 3256, 3268
MhlI	GDGCHC	8 cut(s)	30, 81, 424, 707, 1419, 1608, 3378, 3854
MlsI	TGGCCA	3 cut(s)	54, 63, 3750
MluNI	TGGCCA	3 cut(s)	54, 63, 3750
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	6 cut(s)	437, 1115, 1762, 2838, 2899, 3111
MmeI	TCCRAC	7 cut(s)	22, 126, 882, 1261, 2443, 3195, 3348
Mox20I	TGGCCA	3 cut(s)	54, 63, 3750
Mph1103I	ATGCAT	3 cut(s)	1180, 1693, 3128
MroI	TCCGGA	1 cut(s)	1622
MroXI	GAANNNNTTC	1 cut(s)	3453
MscI	TGGCCA	3 cut(s)	54, 63, 3750
MslI	CAYNNNNRTG	2 cut(s)	667, 2357
Msp20I	TGGCCA	3 cut(s)	54, 63, 3750
MspA1I	CMGCKG	2 cut(s)	1151, 3211
MspCI	CTTAAG	1 cut(s)	3428
MspI	CCGG	7 cut(s)	37, 70, 162, 1575, 1623, 3481, 4053
MspR9I	CCNGG	8 cut(s)	38, 162, 507, 1576, 2343, 2476, 3065, 3620
MunI	CAATTG	3 cut(s)	812, 2184, 3133
MvaI	CCWGG	5 cut(s)	507, 2343, 2476, 3065, 3620
MvnI	CGCG	6 cut(s)	20, 304, 579, 1368, 2089, 4020
MwoI	GCNNNNNNNGC	9 cut(s)	60, 298, 340, 458, 1175, 1688, 2369, 2849, 4007
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	3 cut(s)	38, 162, 1576
NcoI	CCATGG	2 cut(s)	696, 3352
NdeI	CATATG	1 cut(s)	3128
NheI	GCTAGC	1 cut(s)	4037
NmuCI	GTSAC	4 cut(s)	1926, 1953, 2072, 4048
NsiI	ATGCAT	3 cut(s)	1180, 1693, 3128
NspI	RCATGY	2 cut(s)	2456, 2557
NspV	TTCGAA	1 cut(s)	1674
PaeR7I	CTCGAG	2 cut(s)	423, 3798
PagI	TCATGA	3 cut(s)	1033, 1759, 2793
PaqCI	CACCTGC	1 cut(s)	382
PceI	AGGCCT	1 cut(s)	656
PcsI	WCGNNNNNNNCGW	1 cut(s)	164
PdmI	GAANNNNTTC	1 cut(s)	3453
PflFI	GACNNNGTC	1 cut(s)	107
PfoI	TCCNGGA	2 cut(s)	505, 2341
PleI	GAGTC	6 cut(s)	436, 1115, 1762, 2837, 2899, 3110
PluTI	GGCGCC	1 cut(s)	346
PmaCI	CACGTG	1 cut(s)	324
PmlI	CACGTG	1 cut(s)	324
PpsI	GAGTC	6 cut(s)	436, 1115, 1762, 2837, 2899, 3110
Ppu21I	YACGTR	3 cut(s)	324, 1385, 3263
PsiI	TTATAA	1 cut(s)	1466
Psp124BI	GAGCTC	1 cut(s)	424
Psp6I	CCWGG	5 cut(s)	505, 2341, 2474, 3063, 3618
PspCI	CACGTG	1 cut(s)	324
PspFI	CCCAGC	1 cut(s)	3077
PspGI	CCWGG	5 cut(s)	505, 2341, 2474, 3063, 3618
PspPI	GGNCC	4 cut(s)	455, 637, 1823, 2815
PstI	CTGCAG	1 cut(s)	3498
PsuI	RGATCY	5 cut(s)	4, 944, 2099, 3256, 3268
PsyI	GACNNNGTC	1 cut(s)	107
PvuII	CAGCTG	2 cut(s)	1151, 3211
RseI	CAYNNNNRTG	2 cut(s)	667, 2357
SacI	GAGCTC	1 cut(s)	424
SalI	GTCGAC	1 cut(s)	3104
Sau96I	GGNCC	4 cut(s)	455, 637, 1823, 2815
SchI	GAGTC	6 cut(s)	437, 1115, 1762, 2838, 2899, 3111
ScrFI	CCNGG	8 cut(s)	38, 162, 507, 1576, 2343, 2476, 3065, 3620
SduI	GDGCHC	8 cut(s)	30, 81, 424, 707, 1419, 1608, 3378, 3854
SfaNI	GCATC	8 cut(s)	492, 1199, 1678, 2527, 2610, 3412, 3505, 3965
SfcI	CTRYAG	1 cut(s)	3494
SfiI	GGCCNNNNNGGCC	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	344
Sfr274I	CTCGAG	2 cut(s)	423, 3798
SfuI	TTCGAA	1 cut(s)	1674
SinI	GGWCC	4 cut(s)	455, 637, 1823, 2815
SlaI	CTCGAG	2 cut(s)	423, 3798
SmiMI	CAYNNNNRTG	2 cut(s)	667, 2357
SmlI	CTYRAG	6 cut(s)	74, 423, 1306, 3428, 3798, 3809
SmoI	CTYRAG	6 cut(s)	74, 423, 1306, 3428, 3798, 3809
SseBI	AGGCCT	1 cut(s)	656
SspDI	GGCGCC	1 cut(s)	342
SspI	AATATT	4 cut(s)	1347, 1489, 2730, 3054
SspMI	CTAG	5 cut(s)	150, 533, 2280, 2432, 4038
SstI	GAGCTC	1 cut(s)	424
StuI	AGGCCT	1 cut(s)	656
StyD4I	CCNGG	8 cut(s)	36, 160, 505, 1574, 2341, 2474, 3063, 3618
StyI	CCWWGG	8 cut(s)	149, 285, 640, 696, 1665, 2431, 3252, 3352
TaiI	ACGT	9 cut(s)	326, 448, 710, 1387, 1425, 2989, 3265, 3935, 4050
TatI	WGTACW	2 cut(s)	745, 3981
TauI	GCSGC	5 cut(s)	20, 139, 304, 2092, 2902
TscAI	CASTG	8 cut(s)	64, 254, 303, 1297, 1613, 1726, 2916, 3415
TseFI	GTSAC	4 cut(s)	1926, 1953, 2072, 4048
TseI	GCWGC	8 cut(s)	306, 1136, 1679, 2608, 3074, 3185, 3953, 4034
Tsp45I	GTSAC	4 cut(s)	1926, 1953, 2072, 4048
TspGWI	ACGGA	5 cut(s)	1556, 1561, 2738, 2960, 3846
TspRI	CASTG	8 cut(s)	64, 254, 303, 1297, 1613, 1726, 2916, 3415
Tth111I	GACNNNGTC	1 cut(s)	107
Vha464I	CTTAAG	1 cut(s)	3428
VneI	GTGCAC	1 cut(s)	1415
VpaK11BI	GGWCC	4 cut(s)	455, 637, 1823, 2815
XapI	RAATTY	7 cut(s)	794, 1082, 1271, 1831, 1840, 2309, 2324
XbaI	TCTAGA	1 cut(s)	532
XceI	RCATGY	2 cut(s)	2456, 2557
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1054
XhoI	CTCGAG	2 cut(s)	423, 3798
XmaJI	CCTAGG	2 cut(s)	149, 2431
XmiI	GTMKAC	2 cut(s)	1217, 3105
XmnI	GAANNNNTTC	1 cut(s)	3453
XspI	CTAG	5 cut(s)	150, 533, 2280, 2432, 4038
Zsp2I	ATGCAT	3 cut(s)	1180, 1693, 3128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.