pycom11g08370

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009849
Physical Location & Seq
Reverse (-)
6499279 .. 6504130
4852 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
ATGGGTAGCCCAAAGAAGGATCAGAACAAGGGCTTCTTCGCAGCCATGAGTTCCGGCCTCTCCATGTTTGGCAACGCCATGCACCGATCCGTCGGCGGGTTGCTTGGTTATGAAGGGGTGGAAGTCATAAATCCAGAGGGAGGCAAAGAAGATGCAGAGGAGGAAGCTCAAAGAGGAAGATGGAAGCAGGAGGAACGAGACAGTTACTGGAGGATGATGCAGAAGTATATAGGTTCAGATGTAACATCAATGGTGACTCTTCCTGTACTTATCTTTGAGCCAATGACAATGCTGCAGAAAATGGCTGAGTTAATGGAGTATTCACACTTGTTGGATTGCGCCGATGAATGTGAGGATCCTTATATGCAGTTGGTGTTTGCCACATCATGGGCGATATCGGTCTACTATGCCTACCAGCGGACGTGGAAGCCATTCAATCCTATCCTTGGGGAGACTTATGAAATGGTTAATCACTCAGGGGTTACATTTATTGCAGAGCAGGTGAGTCATCATCCACCAATGAGTGCTGGGCATGCAGAAAATGATCATTTCACTTATGATGTGACATCAAAACTGAAGACTAAATTTTTAGGGAACTCTGTTGATGTTTACCCTGTTGGAAGAACACGTGTGACTCTTAAAAGAAATGGTGTAGTCTTAGATTTAGTGCCACCTCCCACAAAGGTGAACAACTTGATATTTGGCCGGACATGGGTTGATTCACCAGGAGAGATGATTATGACCAATCTGACGACTGGGGACAAAGTTGTGCTATATTTTCAGCCATGTGGCTGGTTTGGAGCTGGCCGCTATGAAGTGGATGGATATGTTTATAATGCTGCTGAGGAACCTCAGACACTGATGACTGGGAAATGGAATGAGTCAATGAGTTATCAACCATGTGATTTGGAAGGGGAACCCCTTCCAGGCACAGAATTGAAAGAGGTTTGGCACGTTGCTGATGTTCCAAAGAACGACAAATTTCAGTACACATATTTTGCGCATAAACTAAATAGCTTTGACACCGCTCCTAAAAAGTTTTTGGCATCAGACTCTCGTTTGCGCCCGGATAGATATGCACTTGAGAAGGGTGATCTATCCAAATCTGGTGCAGAAAAAAGCAGATTGGAGGAGAGGCAGAGAGCTGAAAAGAAAGAACGAGAGGCCAAGCATCATCAGTTTACACCAAGATGGTTTGACTTGACTGAGGAAGTTACACCTACACCTTGGGGTGACTTGGAAGTCTATCAATATAACAATAAATACACTGAACACCAAGCCGCAATTGATAGCTCTGACTCAATTGATGTGGTTGATGTTACATCAATTGAATTCAACCCATGGCAGTTTGGTAACCTGTCTGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP

Protein Analysis

457

Amino Acids

52.04

Weight (kDa)

5.12

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 68 - 426 1.4e-111 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
AarI CACCTGC 1 cut(s) 490
AasI GACNNNNNNGTC 1 cut(s) 1241
Acc16I TGCGCA 1 cut(s) 1002
Acc36I ACCTGC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 387
AccBSI CCGCTC 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 402
AciI CCGC 5 cut(s) 96, 418, 808, 1026, 1281
AclWI GGATC 4 cut(s) 27, 81, 350, 363
AcoI YGGCCR 2 cut(s) 703, 805
AcsI RAATTY 3 cut(s) 584, 980, 1331
AcuI CTGAAG 1 cut(s) 596
AcvI CACGTG 1 cut(s) 629
AfaI GTAC 2 cut(s) 267, 989
AfiI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
AflIII ACRYGT 2 cut(s) 626, 628
AgsI TTSAA 4 cut(s) 436, 940, 1331, 1336
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 2 cut(s) 724, 925
AleI CACNNNNGTG 1 cut(s) 683
AluBI AGCT 5 cut(s) 167, 803, 1017, 1145, 1293
AluI AGCT 5 cut(s) 167, 803, 1017, 1145, 1293
Alw26I GTCTC 2 cut(s) 192, 446
AlwI GGATC 4 cut(s) 27, 81, 350, 363
AlwNI CAGNNNCTG 2 cut(s) 207, 859
AoxI GGCC 4 cut(s) 55, 703, 805, 1164
ApeKI GCWGC 3 cut(s) 41, 292, 839
ApoI RAATTY 3 cut(s) 584, 980, 1331
Asp700I GAANNNNTTC 2 cut(s) 431, 921
AspLEI GCGC 3 cut(s) 341, 1003, 1065
AsuC2I CCSGG 1 cut(s) 1067
AsuHPI GGTGA 6 cut(s) 265, 514, 697, 714, 1103, 1244
BamHI GGATCC 1 cut(s) 355
BbrPI CACGTG 1 cut(s) 629
BbsI GAAGAC 1 cut(s) 584
BbvCI CCTCAGC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 53, 279, 826
BccI CCATC 3 cut(s) 174, 815, 1185
BciT130I CCWGG 2 cut(s) 726, 927
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 1 cut(s) 1067
BcoDI GTCTC 2 cut(s) 192, 446
BfmI CTRYAG 2 cut(s) 293, 1362
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 5 cut(s) 42, 293, 808, 840, 1281
BlsI GCNGC 5 cut(s) 43, 294, 809, 841, 1282
Bme1390I CCNGG 3 cut(s) 726, 927, 1067
BmgBI CACGTC 1 cut(s) 423
BmiI GGNNCC 3 cut(s) 357, 849, 918
BmrFI CCNGG 3 cut(s) 726, 927, 1067
BmrI ACTGGG 2 cut(s) 765, 876
BmsI GCATC 4 cut(s) 142, 207, 1055, 1180
BmuI ACTGGG 2 cut(s) 765, 876
BpiI GAAGAC 1 cut(s) 584
BpmI CTGGAG 1 cut(s) 229
Bpu10I CCTNAGC 1 cut(s) 843
BpuEI CTTGAG 1 cut(s) 1103
BpuMI CCSGG 1 cut(s) 1067
BsaAI YACGTR 1 cut(s) 629
BsaJI CCNNGG 3 cut(s) 445, 1226, 1340
Bsc4I CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
Bse1I ACTGG 3 cut(s) 212, 760, 871
BseBI CCWGG 2 cut(s) 726, 927
BseDI CCNNGG 3 cut(s) 445, 1226, 1340
BseGI GGATG 3 cut(s) 219, 511, 826
BseLI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
BseMII CTCAG 5 cut(s) 297, 489, 834, 866, 1197
BseNI ACTGG 3 cut(s) 212, 760, 871
BseRI GAGGAG 2 cut(s) 173, 1145
BseXI GCAGC 3 cut(s) 53, 279, 826
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 1131
BshFI GGCC 4 cut(s) 57, 705, 807, 1166
BsiSI CCGG 3 cut(s) 54, 706, 1067
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 8 cut(s) 16, 96, 140, 387, 417, 446, 712, 926
BsmAI GTCTC 2 cut(s) 192, 446
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 4 cut(s) 57, 705, 807, 1166
Bsp143I GATC 5 cut(s) 19, 86, 355, 544, 1093
Bsp19I CCATGG 1 cut(s) 1340
BspACI CCGC 5 cut(s) 96, 418, 808, 1026, 1281
BspANI GGCC 4 cut(s) 57, 705, 807, 1166
BspCNI CTCAG 5 cut(s) 298, 488, 835, 865, 1198
BspLI GGNNCC 3 cut(s) 357, 849, 918
BspMAI CTGCAG 2 cut(s) 297, 1366
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 4 cut(s) 27, 81, 350, 363
BsrBI CCGCTC 1 cut(s) 1028
BsrI ACTGG 3 cut(s) 212, 760, 871
BssECI CCNNGG 3 cut(s) 445, 1226, 1340
BssMI GATC 5 cut(s) 19, 86, 355, 544, 1093
BssT1I CCWWGG 3 cut(s) 445, 1226, 1340
Bst2UI CCWGG 2 cut(s) 726, 927
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 264
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 534, 805
BstDEI CTNAG 6 cut(s) 306, 475, 658, 843, 852, 1206
BstDSI CCRYGG 1 cut(s) 1340
BstEII GGTNACC 1 cut(s) 1352
BstF5I GGATG 3 cut(s) 219, 511, 826
BstHHI GCGC 3 cut(s) 341, 1003, 1065
BstKTI GATC 5 cut(s) 22, 89, 358, 547, 1096
BstMAI GTCTC 2 cut(s) 192, 446
BstMBI GATC 5 cut(s) 19, 86, 355, 544, 1093
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 2 cut(s) 726, 927
BstNSI RCATGY 1 cut(s) 536
BstPI GGTNACC 1 cut(s) 1352
BstSCI CCNGG 3 cut(s) 724, 925, 1065
BstSFI CTRYAG 2 cut(s) 293, 1362
BstV1I GCAGC 3 cut(s) 53, 279, 826
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 1 cut(s) 355
BstXI CCANNNNNNTGG 1 cut(s) 792
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 4 cut(s) 57, 705, 807, 1166
BtgI CCRYGG 1 cut(s) 1340
BtrI CACGTC 1 cut(s) 423
BtsCI GGATG 3 cut(s) 219, 511, 826
BtsIMutI CAGTG 2 cut(s) 857, 1266
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 534, 805
CaiI CAGNNNCTG 2 cut(s) 207, 859
CfoI GCGC 3 cut(s) 341, 1003, 1065
Csp6I GTAC 2 cut(s) 266, 988
CspCI CAANNNNNGTGG 2 cut(s) 1290, 1325
CviAII CATG 9 cut(s) 46, 64, 79, 387, 533, 711, 786, 900, 1341
CviQI GTAC 2 cut(s) 266, 988
DdeI CTNAG 6 cut(s) 306, 475, 658, 843, 852, 1206
DpnI GATC 5 cut(s) 21, 88, 357, 546, 1095
DpnII GATC 5 cut(s) 19, 86, 355, 544, 1093
DrdI GACNNNNNNGTC 1 cut(s) 1241
DseDI GACNNNNNNGTC 1 cut(s) 1241
EaeI YGGCCR 2 cut(s) 703, 805
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 3 cut(s) 445, 1226, 1340
Eco32I GATATC 1 cut(s) 396
Eco57I CTGAAG 1 cut(s) 596
Eco72I CACGTG 1 cut(s) 629
Eco91I GGTNACC 1 cut(s) 1352
EcoO65I GGTNACC 1 cut(s) 1352
EcoRI GAATTC 1 cut(s) 1331
EcoRII CCWGG 2 cut(s) 724, 925
EcoRV GATATC 1 cut(s) 396
EcoT14I CCWWGG 3 cut(s) 445, 1226, 1340
ErhI CCWWGG 3 cut(s) 445, 1226, 1340
FaeI CATG 9 cut(s) 49, 67, 82, 390, 536, 714, 789, 903, 1344
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FaqI GGGAC 1 cut(s) 773
FatI CATG 9 cut(s) 45, 63, 78, 386, 532, 710, 785, 899, 1340
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 1 cut(s) 544
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 5 cut(s) 42, 293, 808, 840, 1281
FokI GGATG 3 cut(s) 226, 498, 833
Fsp4HI GCNGC 5 cut(s) 42, 293, 808, 840, 1281
FspI TGCGCA 1 cut(s) 1002
GlaI GCGC 3 cut(s) 340, 1002, 1064
GluI GCNGC 5 cut(s) 42, 293, 808, 840, 1281
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 4 cut(s) 57, 705, 807, 1166
HapII CCGG 3 cut(s) 54, 706, 1067
HhaI GCGC 3 cut(s) 341, 1003, 1065
Hin1II CATG 9 cut(s) 49, 67, 82, 390, 536, 714, 789, 903, 1344
Hin6I GCGC 3 cut(s) 339, 1001, 1063
HinP1I GCGC 3 cut(s) 339, 1001, 1063
HinfI GANTC 7 cut(s) 256, 505, 634, 719, 881, 1052, 1298
HpaII CCGG 3 cut(s) 54, 706, 1067
HphI GGTGA 6 cut(s) 265, 514, 697, 714, 1103, 1244
Hpy166II GTNNAC 5 cut(s) 403, 610, 688, 990, 1182
Hpy188I TCNGA 6 cut(s) 24, 238, 750, 855, 1051, 1297
Hpy188III TCNNGA 1 cut(s) 134
Hpy8I GTNNAC 5 cut(s) 403, 610, 688, 990, 1182
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 5 cut(s) 10, 107, 905, 932, 1081
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 3 cut(s) 422, 628, 954
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpyF3I CTNAG 6 cut(s) 306, 475, 658, 843, 852, 1206
HpySE526I ACGT 3 cut(s) 422, 628, 954
Hsp92II CATG 9 cut(s) 49, 67, 82, 390, 536, 714, 789, 903, 1344
HspAI GCGC 3 cut(s) 339, 1001, 1063
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 5 cut(s) 19, 86, 355, 544, 1093
LmnI GCTCC 2 cut(s) 800, 1033
Lsp1109I GCAGC 3 cut(s) 53, 279, 826
LweI GCATC 4 cut(s) 142, 207, 1055, 1180
MaeII ACGT 3 cut(s) 422, 628, 954
MalI GATC 5 cut(s) 21, 88, 357, 546, 1095
MbiI CCGCTC 1 cut(s) 1028
MboI GATC 5 cut(s) 19, 86, 355, 544, 1093
MboII GAAGA 6 cut(s) 28, 161, 189, 251, 589, 633
MfeI CAATTG 3 cut(s) 1284, 1302, 1326
MflI RGATCY 1 cut(s) 355
MluCI AATT 7 cut(s) 584, 935, 980, 1284, 1302, 1326, 1331
MlyI GAGTC 6 cut(s) 250, 514, 628, 890, 1046, 1292
MmeI TCCRAC 2 cut(s) 312, 598
MroXI GAANNNNTTC 2 cut(s) 431, 921
MseI TTAA 3 cut(s) 311, 468, 639
MslI CAYNNNNRTG 2 cut(s) 683, 1189
MspA1I CMGCKG 1 cut(s) 418
MspI CCGG 3 cut(s) 54, 706, 1067
MspR9I CCNGG 3 cut(s) 726, 927, 1067
MunI CAATTG 3 cut(s) 1284, 1302, 1326
MvaI CCWGG 2 cut(s) 726, 927
MwoI GCNNNNNNNGC 1 cut(s) 533
NciI CCSGG 1 cut(s) 1067
NcoI CCATGG 1 cut(s) 1340
NdeII GATC 5 cut(s) 19, 86, 355, 544, 1093
NlaIII CATG 9 cut(s) 49, 67, 82, 390, 536, 714, 789, 903, 1344
NlaIV GGNNCC 3 cut(s) 357, 849, 918
NmuCI GTSAC 4 cut(s) 253, 562, 631, 1232
NsbI TGCGCA 1 cut(s) 1002
NspI RCATGY 1 cut(s) 536
OliI CACNNNNGTG 1 cut(s) 683
PaeI GCATGC 1 cut(s) 536
PaqCI CACCTGC 1 cut(s) 490
PdmI GAANNNNTTC 2 cut(s) 431, 921
PfeI GAWTC 1 cut(s) 719
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 5 cut(s) 43, 294, 809, 841, 1282
PleI GAGTC 6 cut(s) 250, 513, 628, 889, 1046, 1292
PmaCI CACGTG 1 cut(s) 629
PmlI CACGTG 1 cut(s) 629
PpsI GAGTC 6 cut(s) 250, 513, 628, 889, 1046, 1292
Ppu21I YACGTR 1 cut(s) 629
PsiI TTATAA 1 cut(s) 834
Psp6I CCWGG 2 cut(s) 724, 925
PspCI CACGTG 1 cut(s) 629
PspEI GGTNACC 1 cut(s) 1352
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 2 cut(s) 724, 925
PspN4I GGNNCC 3 cut(s) 357, 849, 918
PstI CTGCAG 2 cut(s) 297, 1366
PstNI CAGNNNCTG 2 cut(s) 207, 859
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 2 cut(s) 267, 989
RsaNI GTAC 2 cut(s) 266, 988
RseI CAYNNNNRTG 2 cut(s) 683, 1189
SaqAI TTAA 3 cut(s) 311, 468, 639
SatI GCNGC 5 cut(s) 42, 293, 808, 840, 1281
Sau3AI GATC 5 cut(s) 19, 86, 355, 544, 1093
SchI GAGTC 6 cut(s) 250, 514, 628, 890, 1046, 1292
ScrFI CCNGG 3 cut(s) 726, 927, 1067
SfaNI GCATC 4 cut(s) 142, 207, 1055, 1180
SfcI CTRYAG 2 cut(s) 293, 1362
SmiMI CAYNNNNRTG 2 cut(s) 683, 1189
SmlI CTYRAG 1 cut(s) 1082
SmoI CTYRAG 1 cut(s) 1082
SphI GCATGC 1 cut(s) 536
Sse9I AATT 7 cut(s) 584, 935, 980, 1284, 1302, 1326, 1331
SsiI CCGC 5 cut(s) 96, 418, 808, 1026, 1281
StyD4I CCNGG 3 cut(s) 724, 925, 1065
StyI CCWWGG 3 cut(s) 445, 1226, 1340
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 3 cut(s) 425, 631, 957
TaqII GACCGA 1 cut(s) 388
TasI AATT 7 cut(s) 584, 935, 980, 1284, 1302, 1326, 1331
TatI WGTACW 2 cut(s) 265, 987
TauI GCSGC 2 cut(s) 810, 1283
TfiI GAWTC 1 cut(s) 719
Tru1I TTAA 3 cut(s) 311, 468, 639
Tru9I TTAA 3 cut(s) 311, 468, 639
TscAI CASTG 2 cut(s) 864, 1273
TseFI GTSAC 4 cut(s) 253, 562, 631, 1232
TseI GCWGC 3 cut(s) 41, 292, 839
Tsp45I GTSAC 4 cut(s) 253, 562, 631, 1232
TspDTI ATGAA 4 cut(s) 126, 360, 474, 828
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 864, 1273
Van91I CCANNNNNTGG 1 cut(s) 387
XapI RAATTY 3 cut(s) 584, 980, 1331
XceI RCATGY 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 2 cut(s) 431, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.