pycom11g09540

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007983
Physical Location & Seq
Reverse (-)
7765706 .. 7768522
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1662 bp
ATGGACGGTGATGATCAAAGTAACCGCGAGTTTCCTCGCATGCGGCCTCACCGAATTTTTATTTTCTTTTCCCGGAGAAAAGATCGAGTGCTCCTCAGCTACCCTAACTTCCCCTCTCTTTCGTTCTCTCAACTTTTCAATCTTTTAATTTCGTCGCCGGAGTTTCTTCGCCGGCAAATTGATCGGTCGTCGACCGACCATGGGCCTGCTGCGGCTAAAGCATGCGATGGATTTCATTTATTGAGTATTTGGTACTGGCAGTCTTTCCAGAACAAGGGTTTTTGGATGCCAAAGAGTGGCGGATATGTAAATGAGGGAGATGGAACTTTTGGCAACCCTTCAAGAATTGAGCCAAAGCGATCTCAGCAATGGTTTGTAGATGCTAGTGAAGCAGAGCTGTTTCCAAACAAGAAGCAAGCAGTACATATTCCAAACAGCAAATTGAGCTCGGGAATGTCAAATGAAAATGTCCCTTCATGGGAGAATGCTTCAGGCTTCCAGTCAGTTCCGCATCAATTCATTGACCGGTTATTTGGATCTGATACAGCAAGCTCTGTCAGCTTTGCTGAAAGGAACGTGTCTCCTGTCGGATCAGATAATTGGAATCTAAGGAAGGGTATCGGTGACCAGTTTGGAGAAGACGGATCTGTTAGTTTGTCAGTATCTCATGCTACAGAAGATCCTGAAACTCTTAGCTATGCTGGAATTAGAAAAGTCAAAGTCAATCAGGTAAGGGACGGTGACAATGGCATGCATGCTTCAAGGGAACATGGTTCCAATAGGGAAAATAATAGTAACCTGTCCACAAATCAGGCATTTGATAGGGTAAATGAAACTGGTTTTTTATCAGTCGGGCAGGCCTATGATAAGGAGCACGGTAGTGTCTTACTAATGGGTCATGCCTACAACCAGAGGGATGACCATGGCAGACCAATATGTTCCAACTATGGCAAGGGGGATGAAAGTGTGGTAGCAATGGCTGACAGTTATAATAAAGGGAATGCAAGTATGATTTCTTTTGGGGGGTTTCCTGGTGATCAAGATATAGCCATGGGCAGGCCTGTTGGTAACTATAATCAATTATACCATCCTTCTTCAGTTCAAACATCAGAAACTGCGTGCGAGAAGGAATTGGACACAGCAAATGCCAGTGCAGTTGATAATACTGCCTTTGTAGCCAAAACAAGACCTGAGTCAGTTTCGAAGAATAAACCCGAGGTCAAAACGTCAAGGAAACAAGCTCCAAACAGCTTCCCGTCGAATGTTAGGAGCTTGATTTCTACGGGCATGCTTGATGGGGTTCCTGTAAAATATGTTTCCTTGGCACGTGAGGAGCTTCGCGGGATTATCAAAGGTGTCGGCTATCTATGTGGTTGTCAATCATGTAATTATACTAAGGTGCTTAATGCTTACGAGTTTGAGTGCCATGCTGGTTGCAAAACAAAGCATCCAAACAATCACATATTCTTTGAGAATGGCAAGACCATCTATCAGATAGTGCAAGAATTAAAGAGCACGCCTGAGAGCTTGTTGTTTGATACACTTCAAACTGTGTTTGGTGATCCGATCAATCAGAAGGCTTTTAACAGTTGGAGAGAATCCTTTCAAGCGGCAACTCGTGAACTTCAGCGTATTTATGGAAAGGAGGAACTGAACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

554

Amino Acids

61.61

Weight (kDa)

6.58

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 448 - 503 1.5e-14 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 990
AccB7I CCANNNNNTGG 1 cut(s) 296
AccI GTMKAC 1 cut(s) 191
AccII CGCG 2 cut(s) 27, 1341
AciI CCGC 7 cut(s) 25, 43, 212, 300, 509, 1341, 1610
AclWI GGATC 5 cut(s) 544, 598, 652, 674, 1556
AcsI RAATTY 1 cut(s) 54
AcuI CTGAAG 3 cut(s) 474, 1080, 1610
AcvI CACGTG 1 cut(s) 1328
AfaI GTAC 2 cut(s) 254, 423
AfiI CCNNNNNNNGG 5 cut(s) 201, 274, 296, 478, 1056
AflIII ACRYGT 1 cut(s) 576
AgeI ACCGGT 1 cut(s) 525
AgsI TTSAA 6 cut(s) 139, 342, 762, 1103, 1547, 1607
AjnI CCWGG 1 cut(s) 1030
AleI CACNNNNGTG 1 cut(s) 879
Alw21I GWGCWC 4 cut(s) 93, 449, 876, 1517
Alw26I GTCTC 1 cut(s) 585
AlwI GGATC 5 cut(s) 544, 598, 652, 674, 1556
AlwNI CAGNNNCTG 1 cut(s) 1115
Ama87I CYCGRG 2 cut(s) 448, 1214
AoxI GGCC 4 cut(s) 44, 203, 858, 1058
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 1 cut(s) 54
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
AsiGI ACCGGT 1 cut(s) 525
Asp700I GAANNNNTTC 1 cut(s) 1602
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 73
AsuHPI GGTGA 6 cut(s) 20, 41, 635, 752, 1046, 1571
AsuII TTCGAA 1 cut(s) 1202
AvaI CYCGRG 2 cut(s) 448, 1214
BanII GRGCYC 1 cut(s) 449
BauI CACGAG 1 cut(s) 1617
BbrPI CACGTG 1 cut(s) 1328
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 4 cut(s) 93, 449, 876, 1517
BbvCI CCTCAGC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 196
BccI CCATC 5 cut(s) 221, 314, 1095, 1289, 1493
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 1 cut(s) 1032
BclI TGATCA 2 cut(s) 13, 1036
BcnI CCSGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 1 cut(s) 672
BisI GCNGC 4 cut(s) 44, 210, 213, 1611
BlsI GCNGC 4 cut(s) 45, 211, 214, 1612
Bme1390I CCNGG 2 cut(s) 73, 1032
BmeT110I CYCGRG 2 cut(s) 448, 1214
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 775, 1302
BmrFI CCNGG 2 cut(s) 73, 1032
BmsI GCATC 4 cut(s) 276, 370, 520, 1456
BoxI GACNNNNGTC 1 cut(s) 1192
BpiI GAAGAC 1 cut(s) 645
BplI GAGNNNNNCTC 2 cut(s) 78, 110
Bpu10I CCTNAGC 1 cut(s) 95
Bpu14I TTCGAA 1 cut(s) 1202
BpuMI CCSGG 1 cut(s) 73
BsaAI YACGTR 1 cut(s) 1328
BsaJI CCNNGG 5 cut(s) 199, 922, 1050, 1215, 1320
BsaWI WCCGGW 1 cut(s) 525
Bsc4I CCNNNNNNNGG 5 cut(s) 201, 274, 296, 478, 1056
Bse118I RCCGGY 2 cut(s) 171, 525
Bse1I ACTGG 5 cut(s) 260, 499, 628, 841, 1149
Bse3DI GCAATG 2 cut(s) 374, 981
BseBI CCWGG 1 cut(s) 1032
BseDI CCNNGG 5 cut(s) 199, 922, 1050, 1215, 1320
BseGI GGATG 5 cut(s) 291, 922, 964, 1087, 1447
BseLI CCNNNNNNNGG 5 cut(s) 201, 274, 296, 478, 1056
BseMI GCAATG 2 cut(s) 374, 981
BseMII CTCAG 4 cut(s) 109, 377, 1182, 1512
BseNI ACTGG 5 cut(s) 260, 499, 628, 841, 1149
BseRI GAGGAG 2 cut(s) 83, 1346
BseXI GCAGC 1 cut(s) 196
BsgI GTGCAG 1 cut(s) 1173
Bsh1236I CGCG 2 cut(s) 27, 1341
Bsh1285I CGRYCG 2 cut(s) 188, 195
BshFI GGCC 4 cut(s) 46, 205, 860, 1060
BshTI ACCGGT 1 cut(s) 525
BsiEI CGRYCG 2 cut(s) 188, 195
BsiHKAI GWGCWC 4 cut(s) 93, 449, 876, 1517
BsiHKCI CYCGRG 2 cut(s) 448, 1214
BsiSI CCGG 4 cut(s) 73, 158, 172, 526
BslFI GGGAC 2 cut(s) 455, 749
BslI CCNNNNNNNGG 5 cut(s) 201, 274, 296, 478, 1056
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 2 cut(s) 455, 749
BsmI GAATGC 2 cut(s) 490, 1006
BsnI GGCC 4 cut(s) 46, 205, 860, 1060
BsoBI CYCGRG 2 cut(s) 448, 1214
Bsp119I TTCGAA 1 cut(s) 1202
Bsp1286I GDGCHC 4 cut(s) 93, 449, 876, 1517
Bsp19I CCATGG 3 cut(s) 199, 922, 1050
BspACI CCGC 7 cut(s) 25, 43, 212, 300, 509, 1341, 1610
BspANI GGCC 4 cut(s) 46, 205, 860, 1060
BspCNI CTCAG 4 cut(s) 108, 376, 1183, 1513
BspFNI CGCG 2 cut(s) 27, 1341
BspLI GGNNCC 2 cut(s) 775, 1302
BspPI GGATC 5 cut(s) 544, 598, 652, 674, 1556
BspT104I TTCGAA 1 cut(s) 1202
BsrDI GCAATG 2 cut(s) 374, 981
BsrFI RCCGGY 2 cut(s) 171, 525
BsrI ACTGG 5 cut(s) 260, 499, 628, 841, 1149
BssAI RCCGGY 2 cut(s) 171, 525
BssECI CCNNGG 5 cut(s) 199, 922, 1050, 1215, 1320
BssSI CACGAG 1 cut(s) 1617
BssT1I CCWWGG 4 cut(s) 199, 922, 1050, 1320
Bst2BI CACGAG 1 cut(s) 1617
Bst2UI CCWGG 1 cut(s) 1032
Bst4CI ACNGT 6 cut(s) 8, 740, 878, 986, 1552, 1589
BstBAI YACGTR 1 cut(s) 1328
BstBI TTCGAA 1 cut(s) 1202
BstDEI CTNAG 7 cut(s) 95, 363, 608, 692, 1191, 1395, 1521
BstDSI CCRYGG 3 cut(s) 199, 922, 1050
BstEII GGTNACC 1 cut(s) 623
BstF5I GGATG 5 cut(s) 291, 922, 964, 1087, 1447
BstFNI CGCG 2 cut(s) 27, 1341
BstMAI GTCTC 1 cut(s) 585
BstMCI CGRYCG 2 cut(s) 188, 195
BstMWI GCNNNNNNNGC 6 cut(s) 218, 364, 389, 444, 558, 1175
BstNI CCWGG 1 cut(s) 1032
BstNSI RCATGY 5 cut(s) 43, 225, 754, 758, 1291
BstPAI GACNNNNGTC 1 cut(s) 1192
BstPI GGTNACC 1 cut(s) 623
BstSCI CCNGG 2 cut(s) 71, 1030
BstSFI CTRYAG 1 cut(s) 672
BstUI CGCG 2 cut(s) 27, 1341
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 645
BstX2I RGATCY 3 cut(s) 536, 644, 679
BstYI RGATCY 3 cut(s) 536, 644, 679
BsuRI GGCC 4 cut(s) 46, 205, 860, 1060
BtgI CCRYGG 3 cut(s) 199, 922, 1050
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 5 cut(s) 291, 922, 964, 1087, 1447
BtsIMutI CAGTG 1 cut(s) 1156
CaiI CAGNNNCTG 1 cut(s) 1115
Cfr10I RCCGGY 2 cut(s) 171, 525
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 253, 422
CspAI ACCGGT 1 cut(s) 525
CviQI GTAC 2 cut(s) 253, 422
DdeI CTNAG 7 cut(s) 95, 363, 608, 692, 1191, 1395, 1521
EciI GGCGGA 1 cut(s) 315
Ecl136II GAGCTC 1 cut(s) 447
Eco130I CCWWGG 4 cut(s) 199, 922, 1050, 1320
Eco147I AGGCCT 2 cut(s) 860, 1060
Eco24I GRGCYC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 3 cut(s) 474, 1080, 1610
Eco72I CACGTG 1 cut(s) 1328
Eco88I CYCGRG 2 cut(s) 448, 1214
Eco91I GGTNACC 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 447
EcoO65I GGTNACC 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 1030
EcoT14I CCWWGG 4 cut(s) 199, 922, 1050, 1320
EcoT22I ATGCAT 1 cut(s) 756
EcoT38I GRGCYC 1 cut(s) 449
ErhI CCWWGG 4 cut(s) 199, 922, 1050, 1320
FaqI GGGAC 2 cut(s) 455, 749
FauI CCCGC 1 cut(s) 1334
FbaI TGATCA 2 cut(s) 13, 1036
FblI GTMKAC 1 cut(s) 191
Fnu4HI GCNGC 4 cut(s) 44, 210, 213, 1611
FokI GGATG 5 cut(s) 298, 929, 971, 1074, 1434
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 4 cut(s) 44, 210, 213, 1611
FspBI CTAG 1 cut(s) 384
GluI GCNGC 4 cut(s) 44, 210, 213, 1611
HaeIII GGCC 4 cut(s) 46, 205, 860, 1060
HapII CCGG 4 cut(s) 73, 158, 172, 526
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HinfI GANTC 3 cut(s) 604, 1193, 1598
HpaII CCGG 4 cut(s) 73, 158, 172, 526
HphI GGTGA 6 cut(s) 20, 41, 635, 752, 1046, 1571
Hpy166II GTNNAC 3 cut(s) 192, 804, 1622
Hpy188I TCNGA 7 cut(s) 541, 590, 595, 1111, 1494, 1566, 1575
Hpy188III TCNNGA 6 cut(s) 268, 342, 450, 683, 1040, 1619
Hpy8I GTNNAC 3 cut(s) 192, 804, 1622
Hpy99I CGWCG 3 cut(s) 157, 193, 1261
HpyAV CCTTC 6 cut(s) 348, 483, 607, 1101, 1120, 1570
HpyCH4III ACNGT 6 cut(s) 8, 740, 878, 986, 1552, 1589
HpyCH4IV ACGT 3 cut(s) 576, 1226, 1327
HpyCH4V TGCA 5 cut(s) 754, 1004, 1154, 1437, 1501
HpyF10VI GCNNNNNNNGC 6 cut(s) 218, 364, 389, 444, 558, 1175
HpyF3I CTNAG 7 cut(s) 95, 363, 608, 692, 1191, 1395, 1521
HpySE526I ACGT 3 cut(s) 576, 1226, 1327
KroI GCCGGC 1 cut(s) 171
KroNI GCCGGC 1 cut(s) 173
Ksp22I TGATCA 2 cut(s) 13, 1036
LmnI GCTCC 5 cut(s) 96, 871, 1246, 1269, 1333
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 4 cut(s) 276, 370, 520, 1456
MaeI CTAG 1 cut(s) 384
MaeII ACGT 3 cut(s) 576, 1226, 1327
MaeIII GTNAC 5 cut(s) 20, 623, 740, 794, 1067
MboII GAAGA 5 cut(s) 158, 650, 689, 1086, 1216
MflI RGATCY 3 cut(s) 536, 644, 679
MhlI GDGCHC 4 cut(s) 93, 449, 876, 1517
MlyI GAGTC 1 cut(s) 1202
MmeI TCCRAC 3 cut(s) 568, 966, 1571
MnlI CCTC 9 cut(s) 45, 57, 104, 124, 307, 906, 1210, 1324, 1639
Mph1103I ATGCAT 1 cut(s) 756
MroNI GCCGGC 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 1602
MseI TTAA 4 cut(s) 146, 1404, 1508, 1584
MslI CAYNNNNRTG 1 cut(s) 879
MspI CCGG 4 cut(s) 73, 158, 172, 526
MspR9I CCNGG 2 cut(s) 73, 1032
Mva1269I GAATGC 2 cut(s) 490, 1006
MvaI CCWGG 1 cut(s) 1032
MvnI CGCG 2 cut(s) 27, 1341
MwoI GCNNNNNNNGC 6 cut(s) 218, 364, 389, 444, 558, 1175
NaeI GCCGGC 1 cut(s) 173
NciI CCSGG 1 cut(s) 73
NcoI CCATGG 3 cut(s) 199, 922, 1050
NgoMIV GCCGGC 1 cut(s) 171
NlaIV GGNNCC 2 cut(s) 775, 1302
NmuCI GTSAC 2 cut(s) 623, 740
NsiI ATGCAT 1 cut(s) 756
NspI RCATGY 5 cut(s) 43, 225, 754, 758, 1291
NspV TTCGAA 1 cut(s) 1202
OliI CACNNNNGTG 1 cut(s) 879
PaeI GCATGC 5 cut(s) 43, 225, 754, 758, 1291
PceI AGGCCT 2 cut(s) 860, 1060
PctI GAATGC 2 cut(s) 490, 1006
PdiI GCCGGC 1 cut(s) 173
PdmI GAANNNNTTC 1 cut(s) 1602
PfeI GAWTC 2 cut(s) 604, 1598
PflMI CCANNNNNTGG 1 cut(s) 296
PfoI TCCNGGA 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 525
PkrI GCNGC 4 cut(s) 45, 211, 214, 1612
PleI GAGTC 1 cut(s) 1201
PmaCI CACGTG 1 cut(s) 1328
PmlI CACGTG 1 cut(s) 1328
PpsI GAGTC 1 cut(s) 1201
Ppu21I YACGTR 1 cut(s) 1328
PshAI GACNNNNGTC 1 cut(s) 1192
PsiI TTATAA 1 cut(s) 990
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 1030
PspCI CACGTG 1 cut(s) 1328
PspEI GGTNACC 1 cut(s) 623
PspGI CCWGG 1 cut(s) 1030
PspN4I GGNNCC 2 cut(s) 775, 1302
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 1115
PsuI RGATCY 3 cut(s) 536, 644, 679
RsaI GTAC 2 cut(s) 254, 423
RsaNI GTAC 2 cut(s) 253, 422
RseI CAYNNNNRTG 1 cut(s) 879
SacI GAGCTC 1 cut(s) 449
SalI GTCGAC 1 cut(s) 190
SaqAI TTAA 4 cut(s) 146, 1404, 1508, 1584
SatI GCNGC 4 cut(s) 44, 210, 213, 1611
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 1 cut(s) 1202
ScrFI CCNGG 2 cut(s) 73, 1032
SduI GDGCHC 4 cut(s) 93, 449, 876, 1517
SfaNI GCATC 4 cut(s) 276, 370, 520, 1456
SfcI CTRYAG 1 cut(s) 672
SfuI TTCGAA 1 cut(s) 1202
SmiMI CAYNNNNRTG 1 cut(s) 879
SphI GCATGC 5 cut(s) 43, 225, 754, 758, 1291
SseBI AGGCCT 2 cut(s) 860, 1060
SsiI CCGC 7 cut(s) 25, 43, 212, 300, 509, 1341, 1610
SspMI CTAG 1 cut(s) 384
SstI GAGCTC 1 cut(s) 449
StuI AGGCCT 2 cut(s) 860, 1060
StyD4I CCNGG 2 cut(s) 71, 1030
StyI CCWWGG 4 cut(s) 199, 922, 1050, 1320
TaaI ACNGT 6 cut(s) 8, 740, 878, 986, 1552, 1589
TaiI ACGT 3 cut(s) 579, 1229, 1330
TaqI TCGA 4 cut(s) 85, 191, 1202, 1259
TaqII GACCGA 2 cut(s) 174, 209
TatI WGTACW 1 cut(s) 421
TauI GCSGC 3 cut(s) 46, 215, 1613
TfiI GAWTC 2 cut(s) 604, 1598
Tru1I TTAA 4 cut(s) 146, 1404, 1508, 1584
Tru9I TTAA 4 cut(s) 146, 1404, 1508, 1584
TscAI CASTG 1 cut(s) 1156
TseFI GTSAC 2 cut(s) 623, 740
TseI GCWGC 1 cut(s) 209
Tsp45I GTSAC 2 cut(s) 623, 740
TspDTI ATGAA 6 cut(s) 224, 465, 477, 508, 846, 975
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 1 cut(s) 1156
Van91I CCANNNNNTGG 1 cut(s) 296
XapI RAATTY 1 cut(s) 54
XceI RCATGY 5 cut(s) 43, 225, 754, 758, 1291
XmiI GTMKAC 1 cut(s) 191
XmnI GAANNNNTTC 1 cut(s) 1602
XspI CTAG 1 cut(s) 384
Zsp2I ATGCAT 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.