pycom11g11660

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012598
Physical Location & Seq
Forward (+)
10438483 .. 10439312
830 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGAAACTTGGAAAATTGAACGGATTCAATTATTCCTTCAAGTTTTTCGCTTATAAATATAAGTGCACACAATCTCCTTCTTGTCTCACTCCCAACTCGTTTATATTCCAAAAGAATTACCAAATGGCAAGTAATTTAGGGCTTTTGGTTCCCCTTACCCTCATCGTCTTTAGTGTCGTCTCCCTCCCTCTCCCGTCCACCGCCATCTCCTCCTGCAATGACGCATGCAAAACCCTCGACGACTGCGCTGGCCAGCTCATCTGCATCAACGGCAAGTGTAACGACGACCCCGATATTGGGTCAACAGAATGCGGACACGGCTCTTCACCACCTCCTACGACTGACAACTGTAAGTCGTCAGGAACTCTAGTTTGTGACGGCAAATCATACCCGACATACACATGCTCTCCAGCTGTGACATCATCCACAAAAGCCAGCCTTACTCTCAACGATTTCAGCCAAGGCGGGGATGGCGGTGGCCCGTCATATTGTGACGGAAATTACCATAACAACAGCGAGCGGGTTGTGGCACTTTCCACTGGGTGGTTTAATAATAAGTCGAGGTGTTTTAATATGATAAGAATTACGGCAAGCAATGGGAAGAGTGTGACGGCAAAAGTGGTTGACGAATGTGATTCGCGTGCTGGATGCGATGAGGAGCATGGAGGTCAACCGCCGTGTCAGAACAACATTGTTGATGGATCTTCGGCTGTGTGGGACGCTTTGGGACTGAACAAAGATTTGGGCGTTGTGCCTGTTACTTGGTCCATGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

27.36

Weight (kDa)

5.56

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 112 - 256 5.3e-56 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 54
AccB7I CCANNNNNTGG 1 cut(s) 543
AccBSI CCGCTC 1 cut(s) 520
AccII CGCG 1 cut(s) 640
AciI CCGC 6 cut(s) 201, 312, 465, 474, 520, 674
AclWI GGATC 1 cut(s) 709
AcoI YGGCCR 1 cut(s) 250
AdeI CACNNNGTG 1 cut(s) 543
AfiI CCNNNNNNNGG 4 cut(s) 296, 297, 466, 543
AgsI TTSAA 3 cut(s) 19, 28, 40
AjuI GAANNNNNNNTTGG 2 cut(s) 725, 757
AluBI AGCT 2 cut(s) 256, 413
AluI AGCT 2 cut(s) 256, 413
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 2 cut(s) 89, 184
Alw44I GTGCAC 1 cut(s) 64
AlwI GGATC 1 cut(s) 709
AoxI GGCC 3 cut(s) 250, 478, 771
ApaLI GTGCAC 1 cut(s) 64
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 2 cut(s) 479, 765
AsuHPI GGTGA 1 cut(s) 318
AvaII GGWCC 1 cut(s) 765
BaeGI GKGCMC 1 cut(s) 68
BalI TGGCCA 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 68
BccI CCATC 3 cut(s) 212, 464, 692
BceAI ACGGC 6 cut(s) 286, 334, 394, 603, 627, 661
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BcoDI GTCTC 2 cut(s) 89, 184
BfaI CTAG 2 cut(s) 368, 775
Bme18I GGWCC 1 cut(s) 765
BmgT120I GGNCC 2 cut(s) 479, 765
BmiI GGNNCC 1 cut(s) 150
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 273, 638
BmuI ACTGGG 1 cut(s) 549
BpmI CTGGAG 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 460, 768
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 391, 421
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 297, 466, 543
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 2 cut(s) 223, 601
BseDI CCNNGG 2 cut(s) 460, 768
BseGI GGATG 3 cut(s) 422, 475, 653
BseLI CCNNNNNNNGG 4 cut(s) 296, 297, 466, 543
BseMI GCAATG 2 cut(s) 223, 601
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 2 cut(s) 199, 671
BseSI GKGCMC 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 640
BshFI GGCC 3 cut(s) 252, 480, 773
BsiHKAI GWGCWC 1 cut(s) 68
BslFI GGGAC 2 cut(s) 731, 741
BslI CCNNNNNNNGG 4 cut(s) 296, 297, 466, 543
BsmAI GTCTC 2 cut(s) 89, 184
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 2 cut(s) 731, 741
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 3 cut(s) 252, 480, 773
Bsp1286I GDGCHC 1 cut(s) 68
Bsp143I GATC 1 cut(s) 701
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 6 cut(s) 201, 312, 465, 474, 520, 674
BspANI GGCC 3 cut(s) 252, 480, 773
BspFNI CGCG 1 cut(s) 640
BspLI GGNNCC 1 cut(s) 150
BspPI GGATC 1 cut(s) 709
BspQI GCTCTTC 1 cut(s) 328
BsrBI CCGCTC 1 cut(s) 520
BsrDI GCAATG 2 cut(s) 223, 601
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 2 cut(s) 460, 768
BssMI GATC 1 cut(s) 701
BssT1I CCWWGG 2 cut(s) 460, 768
Bst4CI ACNGT 1 cut(s) 350
Bst6I CTCTTC 2 cut(s) 328, 596
BstC8I GCNNGC 7 cut(s) 226, 250, 254, 436, 518, 592, 642
BstDSI CCRYGG 1 cut(s) 768
BstF5I GGATG 3 cut(s) 422, 475, 653
BstFNI CGCG 1 cut(s) 640
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 1 cut(s) 704
BstMAI GTCTC 2 cut(s) 89, 184
BstMBI GATC 1 cut(s) 701
BstMWI GCNNNNNNNGC 3 cut(s) 270, 318, 471
BstNSI RCATGY 2 cut(s) 228, 405
BstSLI GKGCMC 1 cut(s) 68
BstUI CGCG 1 cut(s) 640
BstX2I RGATCY 1 cut(s) 701
BstYI RGATCY 1 cut(s) 701
BsuRI GGCC 3 cut(s) 252, 480, 773
BtgI CCRYGG 1 cut(s) 768
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 3 cut(s) 422, 475, 653
BtsIMutI CAGTG 1 cut(s) 537
Cac8I GCNNGC 7 cut(s) 226, 250, 254, 436, 518, 592, 642
CfoI GCGC 1 cut(s) 248
Cfr13I GGNCC 2 cut(s) 479, 765
CseI GACGC 2 cut(s) 230, 728
CviAII CATG 4 cut(s) 225, 402, 662, 769
DpnI GATC 1 cut(s) 703
DpnII GATC 1 cut(s) 701
DraIII CACNNNGTG 1 cut(s) 543
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 328, 596
EarI CTCTTC 2 cut(s) 328, 596
Eco130I CCWWGG 2 cut(s) 460, 768
Eco47I GGWCC 1 cut(s) 765
EcoT14I CCWWGG 2 cut(s) 460, 768
ErhI CCWWGG 2 cut(s) 460, 768
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 4 cut(s) 228, 405, 665, 772
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FaqI GGGAC 2 cut(s) 731, 741
FatI CATG 4 cut(s) 224, 401, 661, 768
FauI CCCGC 2 cut(s) 458, 513
FokI GGATG 3 cut(s) 409, 482, 660
FspBI CTAG 2 cut(s) 368, 775
GlaI GCGC 1 cut(s) 247
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 3 cut(s) 252, 480, 773
HgaI GACGC 2 cut(s) 230, 728
HhaI GCGC 1 cut(s) 248
Hin1II CATG 4 cut(s) 228, 405, 665, 772
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HincII GTYRAC 3 cut(s) 303, 625, 671
HindII GTYRAC 3 cut(s) 303, 625, 671
HinfI GANTC 2 cut(s) 24, 635
HphI GGTGA 1 cut(s) 318
Hpy166II GTNNAC 5 cut(s) 66, 198, 303, 625, 671
Hpy188I TCNGA 1 cut(s) 684
Hpy188III TCNNGA 1 cut(s) 360
Hpy8I GTNNAC 5 cut(s) 66, 198, 303, 625, 671
Hpy99I CGWCG 2 cut(s) 242, 287
HpyAV CCTTC 2 cut(s) 46, 87
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 4 cut(s) 66, 216, 228, 264
HpyF10VI GCNNNNNNNGC 3 cut(s) 270, 318, 471
Hsp92II CATG 4 cut(s) 228, 405, 665, 772
HspAI GCGC 1 cut(s) 246
Kzo9I GATC 1 cut(s) 701
LguI GCTCTTC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 658
LpnPI CCDG 9 cut(s) 226, 234, 266, 345, 423, 448, 525, 630, 768
LweI GCATC 2 cut(s) 273, 638
MaeI CTAG 2 cut(s) 368, 775
MaeIII GTNAC 6 cut(s) 278, 374, 415, 491, 607, 757
MalI GATC 1 cut(s) 703
MbiI CCGCTC 1 cut(s) 520
MboI GATC 1 cut(s) 701
MboII GAAGA 3 cut(s) 315, 613, 696
MflI RGATCY 1 cut(s) 701
MhlI GDGCHC 1 cut(s) 68
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 6 cut(s) 14, 28, 115, 133, 499, 582
MluNI TGGCCA 1 cut(s) 252
MnlI CCTC 9 cut(s) 170, 194, 198, 220, 245, 342, 555, 649, 659
Mox20I TGGCCA 1 cut(s) 252
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 252
MseI TTAA 2 cut(s) 549, 570
MslI CAYNNNNRTG 1 cut(s) 400
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 413
Mva1269I GAATGC 1 cut(s) 314
MvnI CGCG 1 cut(s) 640
MwoI GCNNNNNNNGC 3 cut(s) 270, 318, 471
NcoI CCATGG 1 cut(s) 768
NdeII GATC 1 cut(s) 701
NlaIII CATG 4 cut(s) 228, 405, 665, 772
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 4 cut(s) 374, 415, 491, 607
NspI RCATGY 2 cut(s) 228, 405
PaeI GCATGC 1 cut(s) 228
PciSI GCTCTTC 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PctI GAATGC 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 2 cut(s) 24, 635
PflMI CCANNNNNTGG 1 cut(s) 543
PsiI TTATAA 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 2 cut(s) 479, 765
PsuI RGATCY 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 413
RseI CAYNNNNRTG 1 cut(s) 400
SapI GCTCTTC 1 cut(s) 328
SaqAI TTAA 2 cut(s) 549, 570
Sau3AI GATC 1 cut(s) 701
Sau96I GGNCC 2 cut(s) 479, 765
SduI GDGCHC 1 cut(s) 68
SetI ASST 5 cut(s) 258, 334, 415, 566, 670
SfaNI GCATC 2 cut(s) 273, 638
SinI GGWCC 1 cut(s) 765
SmiMI CAYNNNNRTG 1 cut(s) 400
SphI GCATGC 1 cut(s) 228
Sse9I AATT 6 cut(s) 14, 28, 115, 133, 499, 582
SsiI CCGC 6 cut(s) 201, 312, 465, 474, 520, 674
SspMI CTAG 2 cut(s) 368, 775
StyI CCWWGG 2 cut(s) 460, 768
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 2 cut(s) 237, 560
TasI AATT 6 cut(s) 14, 28, 115, 133, 499, 582
TfiI GAWTC 2 cut(s) 24, 635
Tru1I TTAA 2 cut(s) 549, 570
Tru9I TTAA 2 cut(s) 549, 570
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 4 cut(s) 374, 415, 491, 607
Tsp45I GTSAC 4 cut(s) 374, 415, 491, 607
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 36, 510
TspRI CASTG 1 cut(s) 544
Van91I CCANNNNNTGG 1 cut(s) 543
VneI GTGCAC 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 765
XceI RCATGY 2 cut(s) 228, 405
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 2 cut(s) 368, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.