pycom11g14110

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003236
Physical Location & Seq
Forward (+)
13903035 .. 13908541
5507 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1605 bp
ATGACTCGAATTCTGGTTCAGCGAGGTTCTTCGAGTGGTTCTTCTTCCAACCCAACTCGTCCTTCTTCCTTGTCTGGGTCTTCTTCTGCTTGGTCACAGCCACAGGTTAATAATCCTCAGGTTCTTTCGGTTGTGAAAGATGAGGAAATTGTAGAGGAGGCACAAGAAAATGTTATTTTGGATGAGCTGCCAGAGTATTGTGAGAATGGTGACAATAAGGCTGTGAAAAGTGAGGATCTTTTGACAGAAAGCTTTAATGATGACAATGTGAGTCCAAGTGATGAACTAGCTGATGGGGAGAAAGTTGGTAAAAATGACATTGCAAGCCCAGGGGAATTGATGAAAGGTCTAGACGGGCTGAGGATTTCTGAAAGGGTGACAGTTGAAAATGTGGACTTGAGCAGGGATTCTTTACAAAATGTGATTGGGAGCTCTCAACCACCACCTCCTCCAGTCCCTCCCCCAAAACTTCCCTCGGCAAACTCAAGTTCAAGGAGATCTGTCTCAGGAAGTTCAAATGCTGTGCGGATTGGACCTTCTAGAAGAGCAGTTGCCTGGCCTGTTGTTTCAACTAGGACTTCGCCGAATGGGTCGCGGCCTTCTTCTCCCAGGTCTCATGGTGAAAGCGAGGGGTACAATAGTGCTGATGAGCAAAGTCCTTGCTTTGTATCTTCCTATGATGACACTGAAAGAGAACGACAGTTTGAAATTGATATCAGACGTGCAAAAGGTTTTGAAGTGAAAAAAATGCTGGAGGATGGGAATTGTCTCTTTCGTGCTGTTGCAGATCAAGTTTATGGGGATTCTGAAGCCTATGACTTGATCAGGCAAATGTGCATAGATTACATGGAACGAGAGAGGGATCACTTTTCTCAATTTATTACAGAAGGATTTACCTCTTATTGTAAGAGGAAAAGAAGAGACAAGGTATACGGAAACAATGTGGAGATCCAAGCCTTATCTGAAATGTATAATCGGCCCATCCATATTTATTCCTATAGCACAGAACCAATTAATATCTTCCACGGAAGCTATGGCACTGACACACCACCCCTACGATTAAGTTATCATCATGGAAATCATTACAACTCTCTTGTCGATCCCCGTCGTCAGACCATCGGTGTAGGACTTGGGTTCAGCCGTCTTCGTGGGTCAAATGTTGACAAGGATCAGGTCAAGGCTGCTATTAAGGCTCAACAAGACCAACAAATTGATAATGCACTTCTAGCTGAGGGGCGATTTTACTCGGATCTGGAGCTTACTGAGAAAGAAATTGAGCGCATGGTGATGGAAGCTTCAAGGCTTGAATATGCAGACGGCACATTTAAGCAACAACTTGGTCGTAGAGAATCTTCCACTTCTAGTGCTGAGCCATCATCTTCTGGAGCTAGATCATCGGGCAGTGAGACTAAAATGGGAGGGAGGGACAAGGGATTTCAGGACTCGACCCTTAGCAGTAGTATGCAAGTCGTGCTATCGATGGGGTTCAGCTACCTGCAGGTGATCGAGGCATACAGCATATTCGGAGATGATGTTGATTCCATGGTTTGTTACTTGTTGGAAACTAGCAGCAGTGACCGGCGCAAAGGCAAGGCAACTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04622 map04622
Pfam Domains
Protein Families
OTU

Protein Analysis

535

Amino Acids

59.11

Weight (kDa)

5.07

Isoelectric Point (pI)

63.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 253 - 362 1.4e-18 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1489
Acc36I ACCTGC 2 cut(s) 1489, 1503
AccI GTMKAC 1 cut(s) 930
AccII CGCG 1 cut(s) 595
AciI CCGC 2 cut(s) 526, 595
AclWI GGATC 6 cut(s) 243, 870, 943, 1094, 1176, 1257
AcsI RAATTY 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 1 cut(s) 75
AgsI TTSAA 8 cut(s) 386, 492, 516, 570, 707, 737, 1299, 1307
AjiI CACGTC 1 cut(s) 722
AjnI CCWGG 3 cut(s) 328, 554, 608
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 5 cut(s) 508, 618, 773, 915, 1400
AlwI GGATC 6 cut(s) 243, 870, 943, 1094, 1176, 1257
AoxI GGCC 3 cut(s) 557, 596, 977
ApeKI GCWGC 3 cut(s) 187, 1181, 1569
ApoI RAATTY 1 cut(s) 9
AseI ATTAAT 1 cut(s) 1014
AspLEI GCGC 2 cut(s) 1281, 1584
AspS9I GGNCC 2 cut(s) 533, 978
AsuHPI GGTGA 5 cut(s) 221, 388, 632, 1297, 1513
AvaII GGWCC 1 cut(s) 533
AxyI CCTNAGG 1 cut(s) 117
BaeI ACNNNNGTAYC 2 cut(s) 625, 658
BanII GRGCYC 1 cut(s) 434
BarI GAAGNNNNNNTAC 2 cut(s) 1336, 1368
BbsI GAAGAC 2 cut(s) 72, 1136
Bbv12I GWGCWC 1 cut(s) 434
BbvCI CCTCAGC 2 cut(s) 359, 1230
BbvI GCAGC 3 cut(s) 174, 1168, 1581
BccI CCATC 7 cut(s) 287, 752, 989, 1124, 1282, 1381, 1474
BceAI ACGGC 2 cut(s) 1125, 1333
BciT130I CCWGG 3 cut(s) 330, 556, 610
BclI TGATCA 1 cut(s) 822
BcoDI GTCTC 5 cut(s) 508, 618, 773, 915, 1400
BfaI CTAG 8 cut(s) 287, 350, 540, 573, 1226, 1362, 1389, 1566
BfmI CTRYAG 2 cut(s) 997, 1496
BfuAI ACCTGC 2 cut(s) 1489, 1503
BglII AGATCT 1 cut(s) 497
BisI GCNGC 4 cut(s) 188, 596, 1182, 1570
BlpI GCTNAGC 1 cut(s) 1368
BlsI GCNGC 4 cut(s) 189, 597, 1183, 1571
Bme1390I CCNGG 3 cut(s) 330, 556, 610
Bme18I GGWCC 1 cut(s) 533
BmgBI CACGTC 1 cut(s) 722
BmgT120I GGNCC 2 cut(s) 533, 978
BmrFI CCNGG 3 cut(s) 330, 556, 610
BpiI GAAGAC 2 cut(s) 72, 1136
BpmI CTGGAG 4 cut(s) 435, 773, 1274, 1404
Bpu10I CCTNAGC 3 cut(s) 359, 1230, 1451
Bpu1102I GCTNAGC 1 cut(s) 1368
BpuEI CTTGAG 2 cut(s) 418, 469
Bsa29I ATCGAT 1 cut(s) 1478
BsaI GGTCTC 1 cut(s) 618
BsaJI CCNNGG 6 cut(s) 328, 329, 474, 608, 1024, 1542
BsaXI ACNNNNNCTCC 2 cut(s) 1518, 1548
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse118I RCCGGY 1 cut(s) 1578
Bse1I ACTGG 1 cut(s) 452
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 318
BseBI CCWGG 3 cut(s) 330, 556, 610
BseCI ATCGAT 1 cut(s) 1478
BseDI CCNNGG 6 cut(s) 328, 329, 474, 608, 1024, 1542
BseGI GGATG 3 cut(s) 187, 763, 981
BseLI CCNNNNNNNGG 1 cut(s) 75
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 6 cut(s) 131, 350, 519, 1221, 1254, 1359
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 2 cut(s) 170, 438
BseXI GCAGC 3 cut(s) 174, 1168, 1581
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 3 cut(s) 559, 598, 979
BshVI ATCGAT 1 cut(s) 1478
BsiHKAI GWGCWC 1 cut(s) 434
BsiSI CCGG 1 cut(s) 1579
BslFI GGGAC 2 cut(s) 440, 1439
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 5 cut(s) 508, 618, 773, 915, 1400
BsmFI GGGAC 2 cut(s) 440, 1439
BsnI GGCC 3 cut(s) 559, 598, 979
Bso31I GGTCTC 1 cut(s) 618
Bsp1286I GDGCHC 1 cut(s) 434
Bsp1720I GCTNAGC 1 cut(s) 1368
Bsp19I CCATGG 1 cut(s) 1542
BspACI CCGC 2 cut(s) 526, 595
BspANI GGCC 3 cut(s) 559, 598, 979
BspCNI CTCAG 6 cut(s) 130, 351, 518, 1222, 1255, 1360
BspDI ATCGAT 1 cut(s) 1478
BspFNI CGCG 1 cut(s) 595
BspMAI CTGCAG 1 cut(s) 1500
BspMI ACCTGC 2 cut(s) 1489, 1503
BspPI GGATC 6 cut(s) 243, 870, 943, 1094, 1176, 1257
BspQI GCTCTTC 1 cut(s) 538
BspTNI GGTCTC 1 cut(s) 618
BsrDI GCAATG 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 1578
BsrI ACTGG 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 1578
BssECI CCNNGG 6 cut(s) 328, 329, 474, 608, 1024, 1542
BssNAI GTATAC 1 cut(s) 931
BssT1I CCWWGG 1 cut(s) 1542
Bst1107I GTATAC 1 cut(s) 931
Bst2UI CCWGG 3 cut(s) 330, 556, 610
Bst4CI ACNGT 2 cut(s) 382, 702
Bst6I CTCTTC 2 cut(s) 538, 913
BstAPI GCANNNNNTGC 1 cut(s) 1471
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 7 cut(s) 117, 359, 505, 1230, 1263, 1368, 1451
BstDSI CCRYGG 2 cut(s) 1024, 1542
BstF5I GGATG 3 cut(s) 187, 763, 981
BstFNI CGCG 1 cut(s) 595
BstHHI GCGC 2 cut(s) 1281, 1584
BstMAI GTCTC 5 cut(s) 508, 618, 773, 915, 1400
BstMWI GCNNNNNNNGC 3 cut(s) 1190, 1226, 1471
BstNI CCWGG 3 cut(s) 330, 556, 610
BstSCI CCNGG 3 cut(s) 328, 554, 608
BstSFI CTRYAG 2 cut(s) 997, 1496
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 3 cut(s) 174, 1168, 1581
BstV2I GAAGAC 2 cut(s) 72, 1136
BstX2I RGATCY 4 cut(s) 235, 497, 948, 1249
BstYI RGATCY 4 cut(s) 235, 497, 948, 1249
BstZ17I GTATAC 1 cut(s) 931
Bsu15I ATCGAT 1 cut(s) 1478
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 3 cut(s) 559, 598, 979
BsuTUI ATCGAT 1 cut(s) 1478
BtgI CCRYGG 2 cut(s) 1024, 1542
BtrI CACGTC 1 cut(s) 722
BtsCI GGATG 3 cut(s) 187, 763, 981
BtsI GCAGTG 2 cut(s) 1408, 1579
BtsIMutI CAGTG 4 cut(s) 684, 1038, 1408, 1579
BveI ACCTGC 2 cut(s) 1489, 1503
Cac8I GCNNGC 1 cut(s) 325
CfoI GCGC 2 cut(s) 1281, 1584
Cfr10I RCCGGY 1 cut(s) 1578
Cfr13I GGNCC 2 cut(s) 533, 978
ClaI ATCGAT 1 cut(s) 1478
Csp6I GTAC 1 cut(s) 634
CviAII CATG 5 cut(s) 617, 847, 1073, 1282, 1543
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 7 cut(s) 117, 359, 505, 1230, 1263, 1368, 1451
Eam1104I CTCTTC 2 cut(s) 538, 913
EarI CTCTTC 2 cut(s) 538, 913
Ecl136II GAGCTC 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 1542
Eco24I GRGCYC 1 cut(s) 434
Eco31I GGTCTC 1 cut(s) 618
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 1 cut(s) 533
Eco53kI GAGCTC 1 cut(s) 432
Eco57I CTGAAG 1 cut(s) 828
Eco81I CCTNAGG 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 3 cut(s) 328, 554, 608
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 1 cut(s) 1542
EcoT38I GRGCYC 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 1542
FaeI CATG 5 cut(s) 620, 850, 1076, 1285, 1546
FaqI GGGAC 2 cut(s) 440, 1439
FatI CATG 5 cut(s) 616, 846, 1072, 1281, 1542
FbaI TGATCA 1 cut(s) 822
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 4 cut(s) 188, 596, 1182, 1570
FokI GGATG 3 cut(s) 194, 770, 968
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 4 cut(s) 188, 596, 1182, 1570
FspBI CTAG 8 cut(s) 287, 350, 540, 573, 1226, 1362, 1389, 1566
GlaI GCGC 2 cut(s) 1280, 1583
GluI GCNGC 4 cut(s) 188, 596, 1182, 1570
GsuI CTGGAG 4 cut(s) 435, 773, 1274, 1404
HaeIII GGCC 3 cut(s) 559, 598, 979
HapII CCGG 1 cut(s) 1579
HhaI GCGC 2 cut(s) 1281, 1584
Hin1II CATG 5 cut(s) 620, 850, 1076, 1285, 1546
Hin6I GCGC 2 cut(s) 1279, 1582
HinP1I GCGC 2 cut(s) 1279, 1582
HincII GTYRAC 1 cut(s) 1162
HindII GTYRAC 1 cut(s) 1162
HindIII AAGCTT 2 cut(s) 250, 1293
HinfI GANTC 7 cut(s) 4, 271, 407, 803, 1349, 1442, 1538
HpaII CCGG 1 cut(s) 1579
HphI GGTGA 5 cut(s) 221, 388, 632, 1297, 1513
Hpy166II GTNNAC 3 cut(s) 394, 931, 1162
Hpy188I TCNGA 7 cut(s) 370, 719, 808, 964, 1113, 1249, 1526
Hpy188III TCNNGA 6 cut(s) 350, 507, 540, 1253, 1383, 1439
Hpy8I GTNNAC 3 cut(s) 394, 931, 1162
Hpy99I CGWCG 1 cut(s) 1110
HpyAV CCTTC 4 cut(s) 72, 546, 609, 881
HpyCH4III ACNGT 2 cut(s) 382, 702
HpyCH4IV ACGT 1 cut(s) 721
HpyCH4V TGCA 8 cut(s) 323, 725, 785, 837, 1220, 1313, 1465, 1498
HpyF10VI GCNNNNNNNGC 3 cut(s) 1190, 1226, 1471
HpyF3I CTNAG 7 cut(s) 117, 359, 505, 1230, 1263, 1368, 1451
HpySE526I ACGT 1 cut(s) 721
Hsp92II CATG 5 cut(s) 620, 850, 1076, 1285, 1546
HspAI GCGC 2 cut(s) 1279, 1582
Ksp22I TGATCA 1 cut(s) 822
LguI GCTCTTC 1 cut(s) 538
LmnI GCTCC 3 cut(s) 429, 1255, 1385
Lsp1109I GCAGC 3 cut(s) 174, 1168, 1581
MaeI CTAG 8 cut(s) 287, 350, 540, 573, 1226, 1362, 1389, 1566
MaeII ACGT 1 cut(s) 721
MaeIII GTNAC 5 cut(s) 93, 209, 376, 1550, 1574
MflI RGATCY 4 cut(s) 235, 497, 948, 1249
MhlI GDGCHC 1 cut(s) 434
MluCI AATT 9 cut(s) 9, 147, 335, 708, 763, 875, 1011, 1209, 1272
MlyI GAGTC 2 cut(s) 280, 1436
MmeI TCCRAC 2 cut(s) 72, 1539
MseI TTAA 6 cut(s) 108, 255, 1014, 1061, 1188, 1326
MslI CAYNNNNRTG 1 cut(s) 1286
MspI CCGG 1 cut(s) 1579
MspR9I CCNGG 3 cut(s) 330, 556, 610
MvaI CCWGG 3 cut(s) 330, 556, 610
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 3 cut(s) 1190, 1226, 1471
NcoI CCATGG 1 cut(s) 1542
NlaIII CATG 5 cut(s) 620, 850, 1076, 1285, 1546
NmeAIII GCCGAG 1 cut(s) 455
NmuCI GTSAC 4 cut(s) 93, 209, 376, 1574
PaqCI CACCTGC 1 cut(s) 1489
PasI CCCWGGG 1 cut(s) 329
PciSI GCTCTTC 1 cut(s) 538
PfeI GAWTC 4 cut(s) 407, 803, 1349, 1538
PkrI GCNGC 4 cut(s) 189, 597, 1183, 1571
PleI GAGTC 2 cut(s) 279, 1436
PpsI GAGTC 2 cut(s) 279, 1436
PshBI ATTAAT 1 cut(s) 1014
Psp124BI GAGCTC 1 cut(s) 434
Psp6I CCWGG 3 cut(s) 328, 554, 608
PspGI CCWGG 3 cut(s) 328, 554, 608
PspPI GGNCC 2 cut(s) 533, 978
PstI CTGCAG 1 cut(s) 1500
PsuI RGATCY 4 cut(s) 235, 497, 948, 1249
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 1286
SacI GAGCTC 1 cut(s) 434
SapI GCTCTTC 1 cut(s) 538
SaqAI TTAA 6 cut(s) 108, 255, 1014, 1061, 1188, 1326
SatI GCNGC 4 cut(s) 188, 596, 1182, 1570
Sau96I GGNCC 2 cut(s) 533, 978
SbfI CCTGCAGG 1 cut(s) 1500
SchI GAGTC 2 cut(s) 280, 1436
ScrFI CCNGG 3 cut(s) 330, 556, 610
SdaI CCTGCAGG 1 cut(s) 1500
SduI GDGCHC 1 cut(s) 434
SfcI CTRYAG 2 cut(s) 997, 1496
SinI GGWCC 1 cut(s) 533
SmiMI CAYNNNNRTG 1 cut(s) 1286
SmlI CTYRAG 2 cut(s) 397, 484
SmoI CTYRAG 2 cut(s) 397, 484
Sse8387I CCTGCAGG 1 cut(s) 1500
Sse9I AATT 9 cut(s) 9, 147, 335, 708, 763, 875, 1011, 1209, 1272
SsiI CCGC 2 cut(s) 526, 595
SspMI CTAG 8 cut(s) 287, 350, 540, 573, 1226, 1362, 1389, 1566
SstI GAGCTC 1 cut(s) 434
StyD4I CCNGG 3 cut(s) 328, 554, 608
StyI CCWWGG 1 cut(s) 1542
TaaI ACNGT 2 cut(s) 382, 702
TaiI ACGT 1 cut(s) 724
TaqI TCGA 6 cut(s) 7, 32, 1098, 1445, 1478, 1506
TasI AATT 9 cut(s) 9, 147, 335, 708, 763, 875, 1011, 1209, 1272
TauI GCSGC 1 cut(s) 598
TfiI GAWTC 4 cut(s) 407, 803, 1349, 1538
Tru1I TTAA 6 cut(s) 108, 255, 1014, 1061, 1188, 1326
Tru9I TTAA 6 cut(s) 108, 255, 1014, 1061, 1188, 1326
TscAI CASTG 4 cut(s) 691, 1045, 1408, 1579
TseFI GTSAC 4 cut(s) 93, 209, 376, 1574
TseI GCWGC 3 cut(s) 187, 1181, 1569
Tsp45I GTSAC 4 cut(s) 93, 209, 376, 1574
TspDTI ATGAA 2 cut(s) 297, 356
TspGWI ACGGA 2 cut(s) 948, 1041
TspRI CASTG 4 cut(s) 691, 1045, 1408, 1579
VpaK11BI GGWCC 1 cut(s) 533
VspI ATTAAT 1 cut(s) 1014
XapI RAATTY 1 cut(s) 9
XbaI TCTAGA 2 cut(s) 349, 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 1031
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 8 cut(s) 287, 350, 540, 573, 1226, 1362, 1389, 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.