Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

19373282 .. 19378264

Gene Structure 4983 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2877 bp

ATGGATAATCTGAAGGAGCCTCTGTTGAAGCCTTTGGATATTGATAACAAAGATAAAAGAATTAGAACAGTGAAGTTTAAAATAGGGGATATCGAGTGTGCATCTTGTGCAACTACCATTGAATCTGTGTTGGGAAAGCTTGATGGGATCAAGAGTGCAACAGTGTCGCCCATTGAAGGCCAAGCTGCAGTCAATTATATACCAGAGCTCATTAATGCAAGAAAAATTAAGGAAGCTGTAGAAGATGCTGGTTTCCCTGTTTCTGAATTTCCAGAACAAGACGTAGCAGTATGCCGACTGAGGATAAAAGGAATGGCGTGCACCAGCTGCTCCGAGTCTGTTGAGTGCGCTCTTCGAATGGTCGATGGAGTCAAAAATGCAGTTGTTGGTCTAGCCCTTGAAGAAGCAAAGGTTCACTTTGATCCCAATCTCACTGATACTAGTTGCATTATTCAAGCAATTGAAGATGCTGGCTTTGGGGTTGAACTGGTTAGTTCTGGGAATGATGTCAGCAAACTTCATCTAAAACTTGAAGGAGTTGATTCTCCAGAGGATATGACTATTGTCCGATCCTCCGTTGAATCAGTTGAAGGAGTAAGTAATGTTGAAGTCGACTTGGCAGAGAAGAAGGTGACTATTACCTATGATTCAGACTTCACAGGTCCAAGATCTCTTATACGTTGTATTGAAGAAGCTGGCCTTGAATCCAAGGTGTACCAGGTAAGCTTGTATGTTCCTCCAAGACCAAGAGAAGCTGAGCGGAAACATGAGATTCAGATGTACAGGAACCAGTTTTTCCTTAGCTGCCTATTTTCCGTTCCTATATTTTTATTCTCAATGGTGCTTCCGATGCTTCCTCCTTATGGAAACTGGTTAGACTACAAGGTTCACAATATGCTCACTGTAGGGATGCTTTTAAGATGGATTCTTTGCACACCAGTGCAGTTCATTGTTGGCCGGAGGTTCTATGTTGGATCATATCATGCGTTGAGACGTAGATCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTTTACTCTGTCTATATTGCAATAAAAGCATTTGCTTTGGATAAATTTGAAGGGCAAGATTTCTTTGAAACTAGCTCTATGTTGATATCCTTTATACTCTTGGGAAAGTACTTAGAAGTTCTGGCTAAAGGGAAGACATCTGATGCTTTGGCGAAGCTGACAGACCTTGCTCCTGATACGGCTTTTCTGTTGTCATTAGATGACGATGACAATGTTATCTCAGAGATCGAAATCAGCACTCAACTTATTCAAAAAAATGATATACTTAAGGTTGCTCCGGGGGCAAAAGTTCCTGTTGATGGGATTGTAGTTCGTGGTCACAGCTATGTGAATGAGAGTATGATCACAGGAGAGGCAACACCCATTTCTAAAAAACTTGGTGACAAGGTAATTGGTGGCACTATGAATGAGAATGGATGCTTACTGGTGAAGGCAACTCATGTTGGTTCGGAGACTGCACTTTCCCAAATAGTTCAACTTGTTGAAGCTGCACAGCTTGCCAGAGCACCTGTTCAGAAACTAGCTGACCAGATTTCAAAGTTTTTTGTTCCAACGGTTGTCATTGTAGCATTCTTGACATGGCTTGGATGGTTCATCCCTGGAGAATTTGGTCTTTTCCCAGAAAACTGGATACCAAAAGGCATGGATAAATTTGAGCTGGCATTACAATTTGGTATCTCAGTATTAGTGGTTGCTTGCCCTTGTGCCTTGGGACTAGCAACCCCTACGGCAGTAATGGTTGCAACAGGGAAGGGTGCTTCGCAAGGTGTTCTTATCAAGGGTGGAAATGCACTCGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAAACAGGAACTCTGACAGTTGGGAAACCCGTGGTAGTCAATGCAGTGCTCTTCTCCAGTTACTCAATGGAGGAGTTCTGTGCTGTCTCTATTGCCACAGAGGCAAATAGTGAACACCCAATAGCGAAATCGGTGGTGGAGCATGCTAAGAGGCTGCTAAAGACGTTTGGATCAACTGAACATGTGATGGAGGCTAAGGATTTTGAGGTGCACACAGGAGCTGGTGTTAGTGGAAGAGTTGGTGACAAAATGGTTTTGGTTGGGAACAAGAGACTCATGCGGCATAATAATGTCCAGGTTGGTTCTGAGGTTGAGAAATATGTTTCAGAACACGAGAATCTGGCTCAAACATGTGTGTTGGTTGCCGTTGATGGAAAGATTGCCGGGTCGTTTGCTGTAACTGATCCAGTGAAGCCAGAGGCTGCGCGGGTCATCTCTTATCTTCACTCGATGAACATTACTAGCATCATGGTTACTGGTGATAACTGGGCTACAGCAGCAGCCATCGCAAAGGAGGTTGGCATTGATAAGGTGTATGCTGAGACGGATCCACTGGGAAAAGCTGATAGAATCAAAGAATTGCAGATGAAAGGAATGGCGGTGGCAATGGTGGGAGATGGAATCAATGACTCTCCAGCTTTGGTTGCAGCAGATGTTGGCATGGCAATTGGTGCTGGCACCGACGTCGCTATAGAAGCCGCTGATATAGTTCTGATGAAGAGCAACTTGGAAGATGTAGTTACAGCCATAGATGATCGATCCAGAAAAACTATGTCACGAATTCGATTGAATTATGTCTGGGCCCTGGGCTACAATATCCTCGGCATGCCAGTCGCAGCTGGAGTCTTGTTCCCTTTCACTGGAATTCGACTACCACCGTGGCTTGCCGGTGCATGTATGGCTGCTTCATCTCTCAGCGTGGTCTGTTCTTCTCTCTTGTTGCAGTCATATAAGAAGCCTTTGCATATTCAGAGCGTCTCCAACGGCAAGTTTGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

959

Amino Acids

103.61

Weight (kDa)

5.68

Isoelectric Point (pI)

35.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	26 - 85	2.6e-13	Heavy-metal-associated domain
HMA	PF00403	100 - 159	3.7e-09	Heavy-metal-associated domain
HMA	PF00403	189 - 233	1.2e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	424 - 511	8.3e-25	P-type ATPase actuator domain
Hydrolase	PF00702	620 - 844	4.5e-34	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2565
AccB1I	GGYRCC	2 cut(s)	1031, 2555
AccBSI	CCGCTC	1 cut(s)	760
AccI	GTMKAC	1 cut(s)	612
AccII	CGCG	1 cut(s)	2305
AciI	CCGC	5 cut(s)	760, 2158, 2305, 2477, 2577
AclWI	GGATC	9 cut(s)	155, 416, 564, 982, 2056, 2276, 2420, 2433, 2631
AcoI	YGGCCR	1 cut(s)	955
AcsI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2658, 2742
AcuI	CTGAAG	1 cut(s)	32
AcyI	GRCGYC	1 cut(s)	2562
AfaI	GTAC	3 cut(s)	716, 782, 1157
AfiI	CCNNNNNNNGG	4 cut(s)	176, 863, 1346, 2738
AflII	CTTAAG	1 cut(s)	1313
AflIII	ACRYGT	2 cut(s)	2059, 2228
AhlI	ACTAGT	1 cut(s)	440
AjnI	CCWGG	4 cut(s)	717, 1645, 2172, 2682
AjuI	GAANNNNNNNTTGG	2 cut(s)	2588, 2620
AleI	CACNNNNGTG	1 cut(s)	938
Alw21I	GWGCWC	5 cut(s)	210, 323, 1555, 1929, 2091
Alw26I	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2860
Alw44I	GTGCAC	2 cut(s)	319, 2087
AlwI	GGATC	9 cut(s)	155, 416, 564, 982, 2056, 2276, 2420, 2433, 2631
AlwNI	CAGNNNCTG	2 cut(s)	2099, 2300
AoxI	GGCC	4 cut(s)	178, 697, 955, 2679
ApaI	GGGCCC	1 cut(s)	2683
ApaLI	GTGCAC	2 cut(s)	319, 2087
ApoI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2658, 2742
AseI	ATTAAT	1 cut(s)	213
AspLEI	GCGC	2 cut(s)	350, 2305
AspS9I	GGNCC	3 cut(s)	662, 2679, 2680
AsuC2I	CCSGG	2 cut(s)	1326, 2263
AsuHPI	GGTGA	6 cut(s)	643, 1439, 1486, 1870, 2132, 2369
AsuII	TTCGAA	1 cut(s)	355
AvaII	GGWCC	1 cut(s)	662
BaeGI	GKGCMC	4 cut(s)	323, 1034, 2091, 2683
BamHI	GGATCC	1 cut(s)	2425
BanI	GGYRCC	2 cut(s)	1031, 2555
BanII	GRGCYC	2 cut(s)	210, 2683
BarI	GAAGNNNNNNTAC	2 cut(s)	2767, 2799
BauI	CACGAG	1 cut(s)	2210
BbsI	GAAGAC	1 cut(s)	1187
Bbv12I	GWGCWC	5 cut(s)	210, 323, 1555, 1929, 2091
BccI	CCATC	9 cut(s)	137, 359, 915, 1340, 1629, 2059, 2243, 2390, 2489
BceAI	ACGGC	3 cut(s)	1242, 1791, 2228
BcgI	CGANNNNNNTGC	6 cut(s)	147, 181, 2545, 2579, 2692, 2726
BciT130I	CCWGG	4 cut(s)	719, 1647, 2174, 2684
BciVI	GTATCC	1 cut(s)	1671
BclI	TGATCA	1 cut(s)	1389
BcnI	CCSGG	2 cut(s)	1326, 2263
BcoDI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2860
BcuI	ACTAGT	1 cut(s)	440
BfaI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
BfmI	CTRYAG	5 cut(s)	186, 237, 903, 2370, 2568
BfrI	CTTAAG	1 cut(s)	1313
BfuI	GTATCC	1 cut(s)	1671
BglI	GCCNNNNNGGC	1 cut(s)	1979
BglII	AGATCT	1 cut(s)	668
BlpI	GCTNAGC	1 cut(s)	756
BmcAI	AGTACT	1 cut(s)	1157
Bme1390I	CCNGG	6 cut(s)	719, 1326, 1647, 2174, 2263, 2684
Bme18I	GGWCC	1 cut(s)	662
BmgT120I	GGNCC	3 cut(s)	662, 2679, 2680
BmiI	GGNNCC	6 cut(s)	18, 788, 1033, 2427, 2557, 2681
BmrFI	CCNGG	6 cut(s)	719, 1326, 1647, 2174, 2263, 2684
BmrI	ACTGGG	3 cut(s)	1037, 2374, 2441
BmsI	GCATC	8 cut(s)	110, 235, 457, 840, 900, 1180, 1454, 2352
BmuI	ACTGGG	3 cut(s)	1037, 2374, 2441
BoxI	GACNNNNGTC	1 cut(s)	563
BpiI	GAAGAC	1 cut(s)	1187
BpmI	CTGGAG	5 cut(s)	531, 1668, 1918, 2496, 2739
Bpu10I	CCTNAGC	2 cut(s)	800, 2073
Bpu1102I	GCTNAGC	1 cut(s)	756
Bpu14I	TTCGAA	1 cut(s)	355
BpuMI	CCSGG	2 cut(s)	1326, 2263
Bsa29I	ATCGAT	1 cut(s)	2635
BsaBI	GATNNNNATC	2 cut(s)	426, 1277
BsaHI	GRCGYC	1 cut(s)	2562
BsaJI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2682, 2683, 2698, 2756
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 2088, 2118
Bsc4I	CCNNNNNNNGG	4 cut(s)	176, 863, 1346, 2738
Bse118I	RCCGGY	1 cut(s)	2765
Bse3DI	GCAATG	1 cut(s)	2490
Bse8I	GATNNNNATC	2 cut(s)	426, 1277
BseBI	CCWGG	4 cut(s)	719, 1647, 2174, 2684
BseCI	ATCGAT	1 cut(s)	2635
BseDI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2682, 2683, 2698, 2756
BseGI	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BseJI	GATNNNNATC	2 cut(s)	426, 1277
BseLI	CCNNNNNNNGG	4 cut(s)	176, 863, 1346, 2738
BseMI	GCAATG	1 cut(s)	2490
BseMII	CTCAG	7 cut(s)	290, 747, 1281, 1740, 2175, 2409, 2806
BseRI	GAGGAG	1 cut(s)	1964
BseSI	GKGCMC	4 cut(s)	323, 1034, 2091, 2683
BsgI	GTGCAG	3 cut(s)	962, 1488, 1521
Bsh1236I	CGCG	1 cut(s)	2305
BshFI	GGCC	4 cut(s)	180, 699, 957, 2681
BshNI	GGYRCC	2 cut(s)	1031, 2555
BshVI	ATCGAT	1 cut(s)	2635
BsiHKAI	GWGCWC	5 cut(s)	210, 323, 1555, 1929, 2091
BsiSI	CCGG	4 cut(s)	958, 1325, 2262, 2766
BslFI	GGGAC	1 cut(s)	1773
BslI	CCNNNNNNNGG	4 cut(s)	176, 863, 1346, 2738
BsmAI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2860
BsmBI	CGTCTC	3 cut(s)	985, 2414, 2860
BsmFI	GGGAC	1 cut(s)	1773
BsmI	GAATGC	1 cut(s)	1616
BsnI	GGCC	4 cut(s)	180, 699, 957, 2681
Bsp119I	TTCGAA	1 cut(s)	355
Bsp120I	GGGCCC	1 cut(s)	2679
Bsp1286I	GDGCHC	7 cut(s)	210, 323, 1034, 1555, 1929, 2091, 2683
Bsp1407I	TGTACA	1 cut(s)	780
Bsp1720I	GCTNAGC	1 cut(s)	756
BspACI	CCGC	5 cut(s)	760, 2158, 2305, 2477, 2577
BspANI	GGCC	4 cut(s)	180, 699, 957, 2681
BspCNI	CTCAG	7 cut(s)	291, 748, 1280, 1739, 2176, 2410, 2805
BspDI	ATCGAT	1 cut(s)	2635
BspFNI	CGCG	1 cut(s)	2305
BspLI	GGNNCC	6 cut(s)	18, 788, 1033, 2427, 2557, 2681
BspMAI	CTGCAG	1 cut(s)	190
BspPI	GGATC	9 cut(s)	155, 416, 564, 982, 2056, 2276, 2420, 2433, 2631
BspQI	GCTCTTC	3 cut(s)	357, 1934, 2591
BspT104I	TTCGAA	1 cut(s)	355
BspT107I	GGYRCC	2 cut(s)	1031, 2555
BspTI	CTTAAG	1 cut(s)	1313
BsrBI	CCGCTC	1 cut(s)	760
BsrDI	GCAATG	1 cut(s)	2490
BsrFI	RCCGGY	1 cut(s)	2765
BsrGI	TGTACA	1 cut(s)	780
BssAI	RCCGGY	1 cut(s)	2765
BssECI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2682, 2683, 2698, 2756
BssNI	GRCGYC	1 cut(s)	2562
BssSI	CACGAG	1 cut(s)	2210
BssT1I	CCWWGG	2 cut(s)	708, 1755
Bst2BI	CACGAG	1 cut(s)	2210
Bst2UI	CCWGG	4 cut(s)	719, 1647, 2174, 2684
Bst4CI	ACNGT	7 cut(s)	70, 163, 904, 1603, 1867, 1897, 2757
Bst6I	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
BstACI	GRCGYC	1 cut(s)	2562
BstAFI	CTTAAG	1 cut(s)	1313
BstAPI	GCANNNNNTGC	4 cut(s)	107, 327, 1544, 2549
BstAUI	TGTACA	1 cut(s)	780
BstBI	TTCGAA	1 cut(s)	355
BstDSI	CCRYGG	2 cut(s)	1908, 2756
BstF5I	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BstFNI	CGCG	1 cut(s)	2305
BstHHI	GCGC	2 cut(s)	350, 2305
BstMAI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2860
BstNI	CCWGG	4 cut(s)	719, 1647, 2174, 2684
BstNSI	RCATGY	5 cut(s)	2024, 2063, 2232, 2707, 2775
BstPAI	GACNNNNGTC	1 cut(s)	563
BstSCI	CCNGG	6 cut(s)	717, 1324, 1645, 2172, 2261, 2682
BstSFI	CTRYAG	5 cut(s)	186, 237, 903, 2370, 2568
BstSLI	GKGCMC	4 cut(s)	323, 1034, 2091, 2683
BstUI	CGCG	1 cut(s)	2305
BstV2I	GAAGAC	1 cut(s)	1187
BstX2I	RGATCY	2 cut(s)	668, 2425
BstXI	CCANNNNNNTGG	1 cut(s)	1674
BstYI	RGATCY	2 cut(s)	668, 2425
Bsu15I	ATCGAT	1 cut(s)	2635
BsuI	GTATCC	1 cut(s)	1671
BsuRI	GGCC	4 cut(s)	180, 699, 957, 2681
BsuTUI	ATCGAT	1 cut(s)	2635
BtgI	CCRYGG	2 cut(s)	1908, 2756
BtgZI	GCGATG	1 cut(s)	2368
BtsCI	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BtsI	GCAGTG	1 cut(s)	1929
CaiI	CAGNNNCTG	2 cut(s)	2099, 2300
CfoI	GCGC	2 cut(s)	350, 2305
Cfr10I	RCCGGY	1 cut(s)	2765
Cfr13I	GGNCC	3 cut(s)	662, 2679, 2680
ClaI	ATCGAT	1 cut(s)	2635
CseI	GACGC	1 cut(s)	2842
CsiI	ACCWGGT	1 cut(s)	717
Csp6I	GTAC	3 cut(s)	715, 781, 1156
CviQI	GTAC	3 cut(s)	715, 781, 1156
DraI	TTTAAA	1 cut(s)	79
EaeI	YGGCCR	1 cut(s)	955
Eam1104I	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
EarI	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
Ecl136II	GAGCTC	1 cut(s)	208
Eco130I	CCWWGG	2 cut(s)	708, 1755
Eco24I	GRGCYC	2 cut(s)	210, 2683
Eco32I	GATATC	2 cut(s)	91, 1134
Eco47I	GGWCC	1 cut(s)	662
Eco53kI	GAGCTC	1 cut(s)	208
Eco57I	CTGAAG	1 cut(s)	32
EcoICRI	GAGCTC	1 cut(s)	208
EcoO109I	RGGNCCY	1 cut(s)	2680
EcoRI	GAATTC	2 cut(s)	2658, 2742
EcoRII	CCWGG	4 cut(s)	717, 1645, 2172, 2682
EcoRV	GATATC	2 cut(s)	91, 1134
EcoT14I	CCWWGG	2 cut(s)	708, 1755
EcoT38I	GRGCYC	2 cut(s)	210, 2683
ErhI	CCWWGG	2 cut(s)	708, 1755
Esp3I	CGTCTC	3 cut(s)	985, 2414, 2860
FalI	AAGNNNNNCTT	8 cut(s)	401, 433, 684, 716, 1803, 1835, 2588, 2620
FaqI	GGGAC	1 cut(s)	1773
FauI	CCCGC	1 cut(s)	2298
FbaI	TGATCA	1 cut(s)	1389
FblI	GTMKAC	1 cut(s)	612
FokI	GGATG	5 cut(s)	922, 1025, 1476, 1628, 1647
FriOI	GRGCYC	2 cut(s)	210, 2683
FspBI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
GlaI	GCGC	2 cut(s)	349, 2304
GsuI	CTGGAG	5 cut(s)	531, 1668, 1918, 2496, 2739
HaeIII	GGCC	4 cut(s)	180, 699, 957, 2681
HapII	CCGG	4 cut(s)	958, 1325, 2262, 2766
HgaI	GACGC	1 cut(s)	2842
HhaI	GCGC	2 cut(s)	350, 2305
Hin1I	GRCGYC	1 cut(s)	2562
Hin6I	GCGC	2 cut(s)	348, 2303
HinP1I	GCGC	2 cut(s)	348, 2303
HincII	GTYRAC	1 cut(s)	613
HindII	GTYRAC	1 cut(s)	613
HindIII	AAGCTT	2 cut(s)	137, 724
HpaII	CCGG	4 cut(s)	958, 1325, 2262, 2766
HphI	GGTGA	6 cut(s)	643, 1439, 1486, 1870, 2132, 2369
Hpy166II	GTNNAC	7 cut(s)	321, 415, 613, 715, 889, 1991, 2089
Hpy188III	TCNNGA	7 cut(s)	151, 272, 548, 1220, 1621, 2640, 2655
Hpy8I	GTNNAC	7 cut(s)	321, 415, 613, 715, 889, 1991, 2089
Hpy99I	CGWCG	2 cut(s)	2564, 2567
HpyAV	CCTTC	8 cut(s)	7, 170, 527, 584, 622, 1091, 1471, 1792
HpyCH4III	ACNGT	7 cut(s)	70, 163, 904, 1603, 1867, 1897, 2757
HpyCH4IV	ACGT	5 cut(s)	282, 679, 994, 2042, 2562
HpySE526I	ACGT	5 cut(s)	282, 679, 994, 2042, 2562
Hsp92I	GRCGYC	1 cut(s)	2562
HspAI	GCGC	2 cut(s)	348, 2303
Ksp22I	TGATCA	1 cut(s)	1389
LguI	GCTCTTC	3 cut(s)	357, 1934, 2591
LmnI	GCTCC	6 cut(s)	16, 335, 1222, 1327, 2017, 2096
LweI	GCATC	8 cut(s)	110, 235, 457, 840, 900, 1180, 1454, 2352
MabI	ACCWGGT	1 cut(s)	717
MaeI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
MaeII	ACGT	5 cut(s)	282, 679, 994, 2042, 2562
MaeIII	GTNAC	9 cut(s)	631, 1364, 1427, 1937, 2120, 2275, 2350, 2617, 2652
MbiI	CCGCTC	1 cut(s)	760
MfeI	CAATTG	2 cut(s)	459, 2544
MflI	RGATCY	2 cut(s)	668, 2425
MhlI	GDGCHC	7 cut(s)	210, 323, 1034, 1555, 1929, 2091, 2683
MlyI	GAGTC	5 cut(s)	344, 378, 2145, 2501, 2730
MmeI	TCCRAC	2 cut(s)	952, 1622
MseI	TTAA	5 cut(s)	78, 213, 228, 917, 1314
MslI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
MspA1I	CMGCKG	3 cut(s)	327, 2579, 2717
MspCI	CTTAAG	1 cut(s)	1313
MspI	CCGG	4 cut(s)	958, 1325, 2262, 2766
MspR9I	CCNGG	6 cut(s)	719, 1326, 1647, 2174, 2263, 2684
MunI	CAATTG	2 cut(s)	459, 2544
Mva1269I	GAATGC	1 cut(s)	1616
MvaI	CCWGG	4 cut(s)	719, 1647, 2174, 2684
MvnI	CGCG	1 cut(s)	2305
NciI	CCSGG	2 cut(s)	1326, 2263
NlaIV	GGNNCC	6 cut(s)	18, 788, 1033, 2427, 2557, 2681
NmeAIII	GCCGAG	1 cut(s)	2679
NmuCI	GTSAC	5 cut(s)	631, 1364, 1427, 2120, 2652
NspI	RCATGY	5 cut(s)	2024, 2063, 2232, 2707, 2775
NspV	TTCGAA	1 cut(s)	355
OliI	CACNNNNGTG	1 cut(s)	938
PaeI	GCATGC	2 cut(s)	2024, 2707
PasI	CCCWGGG	1 cut(s)	2683
PciI	ACATGT	2 cut(s)	2059, 2228
PciSI	GCTCTTC	3 cut(s)	357, 1934, 2591
PctI	GAATGC	1 cut(s)	1616
PleI	GAGTC	5 cut(s)	343, 377, 2145, 2501, 2729
PpsI	GAGTC	5 cut(s)	343, 377, 2145, 2501, 2729
PscI	ACATGT	2 cut(s)	2059, 2228
PshAI	GACNNNNGTC	1 cut(s)	563
PshBI	ATTAAT	1 cut(s)	213
Psp124BI	GAGCTC	1 cut(s)	210
Psp6I	CCWGG	4 cut(s)	717, 1645, 2172, 2682
PspGI	CCWGG	4 cut(s)	717, 1645, 2172, 2682
PspN4I	GGNNCC	6 cut(s)	18, 788, 1033, 2427, 2557, 2681
PspOMI	GGGCCC	1 cut(s)	2679
PspPI	GGNCC	3 cut(s)	662, 2679, 2680
PstI	CTGCAG	1 cut(s)	190
PstNI	CAGNNNCTG	2 cut(s)	2099, 2300
PsuI	RGATCY	2 cut(s)	668, 2425
PvuII	CAGCTG	2 cut(s)	327, 2717
RsaI	GTAC	3 cut(s)	716, 782, 1157
RsaNI	GTAC	3 cut(s)	715, 781, 1156
RseI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
SacI	GAGCTC	1 cut(s)	210
SalI	GTCGAC	1 cut(s)	611
SapI	GCTCTTC	3 cut(s)	357, 1934, 2591
SaqAI	TTAA	5 cut(s)	78, 213, 228, 917, 1314
Sau96I	GGNCC	3 cut(s)	662, 2679, 2680
ScaI	AGTACT	1 cut(s)	1157
SchI	GAGTC	5 cut(s)	344, 378, 2145, 2501, 2730
ScrFI	CCNGG	6 cut(s)	719, 1326, 1647, 2174, 2263, 2684
SduI	GDGCHC	7 cut(s)	210, 323, 1034, 1555, 1929, 2091, 2683
SexAI	ACCWGGT	1 cut(s)	717
SfaNI	GCATC	8 cut(s)	110, 235, 457, 840, 900, 1180, 1454, 2352
SfcI	CTRYAG	5 cut(s)	186, 237, 903, 2370, 2568
SfuI	TTCGAA	1 cut(s)	355
SinI	GGWCC	1 cut(s)	662
SmiMI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
SmlI	CTYRAG	1 cut(s)	1313
SmoI	CTYRAG	1 cut(s)	1313
SpeI	ACTAGT	1 cut(s)	440
SphI	GCATGC	2 cut(s)	2024, 2707
SsiI	CCGC	5 cut(s)	760, 2158, 2305, 2477, 2577
SspMI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
SstI	GAGCTC	1 cut(s)	210
StyD4I	CCNGG	6 cut(s)	717, 1324, 1645, 2172, 2261, 2682
StyI	CCWWGG	2 cut(s)	708, 1755
TaaI	ACNGT	7 cut(s)	70, 163, 904, 1603, 1867, 1897, 2757
TaiI	ACGT	5 cut(s)	285, 682, 997, 2045, 2565
TatI	WGTACW	2 cut(s)	780, 1155
TauI	GCSGC	2 cut(s)	2161, 2579
Tru1I	TTAA	5 cut(s)	78, 213, 228, 917, 1314
Tru9I	TTAA	5 cut(s)	78, 213, 228, 917, 1314
TseFI	GTSAC	5 cut(s)	631, 1364, 1427, 2120, 2652
Tsp45I	GTSAC	5 cut(s)	631, 1364, 1427, 2120, 2652
TspDTI	ATGAA	8 cut(s)	509, 937, 1466, 1630, 2345, 2480, 2609, 2775
TspGWI	ACGGA	3 cut(s)	565, 805, 2438
Vha464I	CTTAAG	1 cut(s)	1313
VneI	GTGCAC	2 cut(s)	319, 2087
VpaK11BI	GGWCC	1 cut(s)	662
VspI	ATTAAT	1 cut(s)	213
XapI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2658, 2742
XceI	RCATGY	5 cut(s)	2024, 2063, 2232, 2707, 2775
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1942
XmiI	GTMKAC	1 cut(s)	612
XspI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
ZraI	GACGTC	1 cut(s)	2563
ZrmI	AGTACT	1 cut(s)	1157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.