pycom11g17260
NAC Family

Inactive beta-amylase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008239
Physical Location & Seq
Reverse (-)
19382801 .. 19386942
4142 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1578 bp
ATGGCAATGGCGGACAATGGGCGAGTGGTCTGCAAATGCACGGCAGCGAGGAGGAGCTTCTTCTGCTTCAGAGAAGTCAGCTTCGATGACAAGCCCAACCGCCGAAACCACCTCCGTAACGTTTCGGCCGTTCCCGTCTTTAAAAACGGCATCTTCACTCGCTGCAGCCCATCTGTCGCCGGAAACAGCATTCTCAGTATGGATGCACGCGAGAATCCGAGGTCCTCAATGTTGCCATCATCCCGAGATAAGAGAATTCCCATATATGTGATGATGCCTATTGATGCTTTCTGCATTGATGGTTCGGGGCGTCCGAGGATTAGGAAAATCAAGGCCTTAACTGTAGCTCTTAAAGCACTCAAGTTGGCAGGTGTCCACGGAATTGCAGTTGAGGTTTGGTGGGGAATCGTAGAGCGTTTCTCTCCTCTTGCATATGATTGGTCTCTATACGAAGAGCTTTTCAAACTGATTTCTGAGTCAGGTTTGAAGTTGCATGTTGCCTTGTCTTTTCATTCAAATGTTAACTCTTCTTCTAGCAGAAAAGGAGGTGTAAGTCTTCCGCTGTGGATCATAGAGATCGGTGATCAGAATAAGCATATATATTATCGAGATCAAAAAGGGTTTTCCAATGATGATTATCTTACACTCGGAGTTGATCATGTTCCTCTCTTTTGTGGCCGGACTGCCCTCCAATGTTATGAAGATTTCATGTCAAATTTTGCTAAGAAATTTGAATCTTTTATCGGAACTGTGATAGAAGAAATTAGTGTTGGCCTTGGTCCTTCTGGTGAACTTAGATATCCTGCACATCCTTTTGGTGATGGTAGATGGAAGTTTCCTGGAATCGGTGAATTCCAATGCTATGACAAGTACATGATGGATGACCTAAAGATGGCTGCATGCAAGGAAGGAAAGCCTCAGTGGGGAGAGAGGGGACCACAAAATGCTGGCTGTTACAACAGTCTCCCATCTGAAGTTCCTTTCTTTGAAGAGGGAGAGGAAAGCTTTCTTTCTGATTATGGGTGTTTTTTCCTTGAATGGTACAGTGGTAGGTTGCTCCGTCATGCAGATGATATTCTTGCAAAGGCAGCCAACTTTCTGAAGAAATATCAAGAAAACAAGCAAACTAATATTCTATTGGCGGCAAAAATTGGTGGAATATATTGGTGGTACCAGACAGTATCACACCCTGCTGAACTTACAGCTGGATACTACAACACTGCTCTTAGAGATGGTTATGATCCCGTTGCTTCAATTTTGTCTCGTCATGGAGCTGCTTTGCATGTTTCCTGCTTAGAAATGACGGATGGTGATAACCCAGCATCCTATCTTTGTAGCCCAGAAGGATTACTCCAGCAGATATGGACTGTTTCAAAGAAAAGGGTACATTTGATCGGCAGAAATACCAATGAACGATTTGACAGGGTTGGTTTATGGCAAATACATGCTAACTGTTACCATTCCCAGGCTGAAGCTGTAAGATCATTTACGTATTTCAGAATGAATGATAAGATCTTTAGGGCCGAAAATTGGAATAACTTCGTTCCTTTTGTTAGAAAGATGAGCGCAAATTGGTGA

Protein Analysis

526

Amino Acids

59.67

Weight (kDa)

8.61

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 90 - 517 1.5e-108 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 359
Acc36I ACCTGC 1 cut(s) 359
Acc65I GGTACC 1 cut(s) 1170
AccB1I GGYRCC 1 cut(s) 1170
AccII CGCG 1 cut(s) 210
AciI CCGC 4 cut(s) 11, 100, 560, 1142
AclI AACGTT 1 cut(s) 120
AclWI GGATC 2 cut(s) 575, 1235
AcoI YGGCCR 2 cut(s) 126, 676
AcsI RAATTY 4 cut(s) 255, 715, 728, 851
AcuI CTGAAG 4 cut(s) 52, 993, 1121, 1491
AcyI GRCGYC 1 cut(s) 310
AfaI GTAC 4 cut(s) 872, 1043, 1172, 1386
AfiI CCNNNNNNNGG 5 cut(s) 845, 892, 923, 1465, 1530
AgsI TTSAA 8 cut(s) 463, 487, 516, 734, 989, 1037, 1254, 1374
AjnI CCWGG 2 cut(s) 838, 1464
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AluBI AGCT 8 cut(s) 57, 81, 347, 457, 1005, 1205, 1274, 1475
AluI AGCT 8 cut(s) 57, 81, 347, 457, 1005, 1205, 1274, 1475
Alw26I GTCTC 3 cut(s) 447, 968, 1266
AlwI GGATC 2 cut(s) 575, 1235
Ama87I CYCGRG 1 cut(s) 243
AoxI GGCC 5 cut(s) 126, 333, 676, 772, 1521
ApeKI GCWGC 6 cut(s) 44, 162, 165, 896, 1088, 1274
ApoI RAATTY 4 cut(s) 255, 715, 728, 851
Asp700I GAANNNNTTC 2 cut(s) 1005, 1538
Asp718I GGTACC 1 cut(s) 1170
AspLEI GCGC 1 cut(s) 1568
AspS9I GGNCC 4 cut(s) 222, 779, 935, 1521
AsuHPI GGTGA 5 cut(s) 593, 800, 830, 860, 1322
AvaI CYCGRG 1 cut(s) 243
AvaII GGWCC 3 cut(s) 222, 779, 935
BaeI ACNNNNGTAYC 2 cut(s) 1033, 1066
BanI GGYRCC 1 cut(s) 1170
BbsI GAAGAC 1 cut(s) 548
BbvI GCAGC 6 cut(s) 56, 149, 177, 883, 1100, 1261
BceAI ACGGC 3 cut(s) 57, 113, 163
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 2 cut(s) 840, 1466
BciVI GTATCC 1 cut(s) 1202
BclI TGATCA 2 cut(s) 583, 655
BcoDI GTCTC 3 cut(s) 447, 968, 1266
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 2 cut(s) 163, 342
BfuAI ACCTGC 1 cut(s) 359
BfuI GTATCC 1 cut(s) 1202
BglII AGATCT 1 cut(s) 1512
BisI GCNGC 7 cut(s) 45, 163, 166, 897, 1089, 1143, 1275
BlsI GCNGC 7 cut(s) 46, 164, 167, 898, 1090, 1144, 1276
Bme1390I CCNGG 2 cut(s) 840, 1466
Bme18I GGWCC 3 cut(s) 222, 779, 935
BmeT110I CYCGRG 1 cut(s) 243
BmgT120I GGNCC 4 cut(s) 222, 779, 935, 1521
BmiI GGNNCC 2 cut(s) 936, 1172
BmrFI CCNGG 2 cut(s) 840, 1466
BmsI GCATC 5 cut(s) 159, 193, 264, 274, 1331
BpiI GAAGAC 1 cut(s) 548
BplI GAGNNNNNCTC 2 cut(s) 404, 436
BpmI CTGGAG 1 cut(s) 1337
BpuEI CTTGAG 1 cut(s) 344
BsaAI YACGTR 1 cut(s) 1491
BsaBI GATNNNNATC 1 cut(s) 636
BsaHI GRCGYC 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 5 cut(s) 218, 314, 376, 775, 1464
Bsc4I CCNNNNNNNGG 5 cut(s) 845, 892, 923, 1465, 1530
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 2 cut(s) 840, 1466
BseDI CCNNGG 5 cut(s) 218, 314, 376, 775, 1464
BseGI GGATG 6 cut(s) 208, 239, 808, 886, 1312, 1322
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 5 cut(s) 845, 892, 923, 1465, 1530
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 208, 465, 932
BseRI GAGGAG 3 cut(s) 64, 67, 414
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 6 cut(s) 56, 149, 177, 883, 1100, 1261
BseYI CCCAGC 1 cut(s) 1318
BsgI GTGCAG 1 cut(s) 789
Bsh1236I CGCG 1 cut(s) 210
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 5 cut(s) 128, 335, 678, 774, 1523
BshNI GGYRCC 1 cut(s) 1170
BsiEI CGRYCG 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 2 cut(s) 180, 679
BslFI GGGAC 1 cut(s) 948
BslI CCNNNNNNNGG 5 cut(s) 845, 892, 923, 1465, 1530
BsmAI GTCTC 3 cut(s) 447, 968, 1266
BsmFI GGGAC 1 cut(s) 948
BsmI GAATGC 1 cut(s) 189
BsnI GGCC 5 cut(s) 128, 335, 678, 774, 1523
Bso31I GGTCTC 1 cut(s) 447
BsoBI CYCGRG 1 cut(s) 243
Bsp143I GATC 9 cut(s) 567, 576, 583, 610, 655, 1240, 1392, 1481, 1512
BspACI CCGC 4 cut(s) 11, 100, 560, 1142
BspANI GGCC 5 cut(s) 128, 335, 678, 774, 1523
BspCNI CTCAG 3 cut(s) 207, 466, 931
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 936, 1172
BspMAI CTGCAG 1 cut(s) 167
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 2 cut(s) 575, 1235
BspQI GCTCTTC 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 1170
BspTNI GGTCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 12
BssECI CCNNGG 5 cut(s) 218, 314, 376, 775, 1464
BssMI GATC 9 cut(s) 567, 576, 583, 610, 655, 1240, 1392, 1481, 1512
BssNI GRCGYC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 775
Bst2UI CCWGG 2 cut(s) 840, 1466
Bst4CI ACNGT 7 cut(s) 343, 751, 962, 1046, 1180, 1369, 1454
Bst6I CTCTTC 3 cut(s) 447, 532, 984
BstACI GRCGYC 1 cut(s) 310
BstBAI YACGTR 1 cut(s) 1491
BstC8I GCNNGC 3 cut(s) 208, 901, 949
BstDEI CTNAG 7 cut(s) 194, 474, 723, 794, 918, 1226, 1294
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 6 cut(s) 208, 239, 808, 886, 1312, 1322
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 1568
BstKTI GATC 9 cut(s) 570, 579, 586, 613, 658, 1243, 1395, 1484, 1515
BstMAI GTCTC 3 cut(s) 447, 968, 1266
BstMBI GATC 9 cut(s) 567, 576, 583, 610, 655, 1240, 1392, 1481, 1512
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 3 cut(s) 63, 353, 1088
BstNI CCWGG 2 cut(s) 840, 1466
BstNSI RCATGY 4 cut(s) 497, 903, 1286, 1448
BstSCI CCNGG 2 cut(s) 838, 1464
BstSFI CTRYAG 2 cut(s) 163, 342
BstSNI TACGTA 1 cut(s) 1491
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 6 cut(s) 56, 149, 177, 883, 1100, 1261
BstV2I GAAGAC 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 1512
BstYI RGATCY 1 cut(s) 1512
BstZI CGGCCG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 1202
BsuRI GGCC 5 cut(s) 128, 335, 678, 774, 1523
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 6 cut(s) 208, 239, 808, 886, 1312, 1322
BtsI GCAGTG 1 cut(s) 1218
BtsIMutI CAGTG 3 cut(s) 926, 1051, 1218
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 3 cut(s) 208, 901, 949
CfoI GCGC 1 cut(s) 1568
Cfr13I GGNCC 4 cut(s) 222, 779, 935, 1521
CseI GACGC 1 cut(s) 299
Csp6I GTAC 4 cut(s) 871, 1042, 1171, 1385
CviAII CATG 9 cut(s) 494, 659, 709, 874, 900, 1064, 1268, 1283, 1445
CviQI GTAC 4 cut(s) 871, 1042, 1171, 1385
DdeI CTNAG 7 cut(s) 194, 474, 723, 794, 918, 1226, 1294
DpnI GATC 9 cut(s) 569, 578, 585, 612, 657, 1242, 1394, 1483, 1514
DpnII GATC 9 cut(s) 567, 576, 583, 610, 655, 1240, 1392, 1481, 1512
DraI TTTAAA 1 cut(s) 142
EaeI YGGCCR 2 cut(s) 126, 676
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 3 cut(s) 447, 532, 984
EarI CTCTTC 3 cut(s) 447, 532, 984
EciI GGCGGA 1 cut(s) 26
EclXI CGGCCG 1 cut(s) 126
Eco105I TACGTA 1 cut(s) 1491
Eco130I CCWWGG 1 cut(s) 775
Eco147I AGGCCT 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 447
Eco32I GATATC 1 cut(s) 800
Eco47I GGWCC 3 cut(s) 222, 779, 935
Eco52I CGGCCG 1 cut(s) 126
Eco57I CTGAAG 4 cut(s) 52, 993, 1121, 1491
Eco88I CYCGRG 1 cut(s) 243
EcoO109I RGGNCCY 1 cut(s) 222
EcoRI GAATTC 2 cut(s) 255, 851
EcoRII CCWGG 2 cut(s) 838, 1464
EcoRV GATATC 1 cut(s) 800
EcoT14I CCWWGG 1 cut(s) 775
ErhI CCWWGG 1 cut(s) 775
FaeI CATG 9 cut(s) 497, 662, 712, 877, 903, 1067, 1271, 1286, 1448
FaqI GGGAC 1 cut(s) 948
FatI CATG 9 cut(s) 493, 658, 708, 873, 899, 1063, 1267, 1282, 1444
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 2 cut(s) 583, 655
Fnu4HI GCNGC 7 cut(s) 45, 163, 166, 897, 1089, 1143, 1275
FokI GGATG 6 cut(s) 215, 226, 795, 893, 1309, 1319
Fsp4HI GCNGC 7 cut(s) 45, 163, 166, 897, 1089, 1143, 1275
FspBI CTAG 1 cut(s) 534
GlaI GCGC 1 cut(s) 1567
GluI GCNGC 7 cut(s) 45, 163, 166, 897, 1089, 1143, 1275
GsaI CCCAGC 1 cut(s) 1322
GsuI CTGGAG 1 cut(s) 1337
HaeIII GGCC 5 cut(s) 128, 335, 678, 774, 1523
HapII CCGG 2 cut(s) 180, 679
HgaI GACGC 1 cut(s) 299
HhaI GCGC 1 cut(s) 1568
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 9 cut(s) 497, 662, 712, 877, 903, 1067, 1271, 1286, 1448
Hin6I GCGC 1 cut(s) 1566
HinP1I GCGC 1 cut(s) 1566
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 1003
HinfI GANTC 5 cut(s) 214, 405, 476, 734, 843
HpaI GTTAAC 1 cut(s) 523
HpaII CCGG 2 cut(s) 180, 679
HphI GGTGA 5 cut(s) 593, 800, 830, 860, 1322
Hpy166II GTNNAC 3 cut(s) 376, 523, 791
Hpy188III TCNNGA 3 cut(s) 243, 608, 1112
Hpy8I GTNNAC 3 cut(s) 376, 523, 791
HpyAV CCTTC 3 cut(s) 792, 902, 1337
HpyCH4III ACNGT 7 cut(s) 343, 751, 962, 1046, 1180, 1369, 1454
HpyCH4IV ACGT 2 cut(s) 120, 1490
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 353, 1088
HpyF3I CTNAG 7 cut(s) 194, 474, 723, 794, 918, 1226, 1294
HpySE526I ACGT 2 cut(s) 120, 1490
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 9 cut(s) 497, 662, 712, 877, 903, 1067, 1271, 1286, 1448
HspAI GCGC 1 cut(s) 1566
KpnI GGTACC 1 cut(s) 1174
Ksp22I TGATCA 2 cut(s) 583, 655
KspAI GTTAAC 1 cut(s) 523
Kzo9I GATC 9 cut(s) 567, 576, 583, 610, 655, 1240, 1392, 1481, 1512
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 3 cut(s) 54, 1062, 1271
Lsp1109I GCAGC 6 cut(s) 56, 149, 177, 883, 1100, 1261
LweI GCATC 5 cut(s) 159, 193, 264, 274, 1331
MaeI CTAG 1 cut(s) 534
MaeII ACGT 2 cut(s) 120, 1490
MaeIII GTNAC 3 cut(s) 116, 953, 1454
MalI GATC 9 cut(s) 569, 578, 585, 612, 657, 1242, 1394, 1483, 1514
MboI GATC 9 cut(s) 567, 576, 583, 610, 655, 1240, 1392, 1481, 1512
MflI RGATCY 1 cut(s) 1512
MlyI GAGTC 1 cut(s) 485
MroXI GAANNNNTTC 2 cut(s) 1005, 1538
MseI TTAA 4 cut(s) 141, 338, 351, 522
MslI CAYNNNNRTG 3 cut(s) 266, 516, 1068
MspA1I CMGCKG 2 cut(s) 562, 1205
MspI CCGG 2 cut(s) 180, 679
MspR9I CCNGG 2 cut(s) 840, 1466
Mva1269I GAATGC 1 cut(s) 189
MvaI CCWGG 2 cut(s) 840, 1466
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 3 cut(s) 63, 353, 1088
NdeI CATATG 1 cut(s) 433
NdeII GATC 9 cut(s) 567, 576, 583, 610, 655, 1240, 1392, 1481, 1512
NlaIII CATG 9 cut(s) 497, 662, 712, 877, 903, 1067, 1271, 1286, 1448
NlaIV GGNNCC 2 cut(s) 936, 1172
NspI RCATGY 4 cut(s) 497, 903, 1286, 1448
PaeI GCATGC 1 cut(s) 903
PaqCI CACCTGC 1 cut(s) 359
PceI AGGCCT 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 447
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 311
PctI GAATGC 1 cut(s) 189
PdmI GAANNNNTTC 2 cut(s) 1005, 1538
PfeI GAWTC 4 cut(s) 214, 405, 734, 843
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 7 cut(s) 46, 164, 167, 898, 1090, 1144, 1276
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
Ppu21I YACGTR 1 cut(s) 1491
PpuMI RGGWCCY 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 222
Psp6I CCWGG 2 cut(s) 838, 1464
PspFI CCCAGC 1 cut(s) 1318
PspGI CCWGG 2 cut(s) 838, 1464
PspN4I GGNNCC 2 cut(s) 936, 1172
PspPI GGNCC 4 cut(s) 222, 779, 935, 1521
PspPPI RGGWCCY 1 cut(s) 222
PstI CTGCAG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 1512
PvuII CAGCTG 1 cut(s) 1205
RsaI GTAC 4 cut(s) 872, 1043, 1172, 1386
RsaNI GTAC 4 cut(s) 871, 1042, 1171, 1385
RseI CAYNNNNRTG 3 cut(s) 266, 516, 1068
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 4 cut(s) 141, 338, 351, 522
SatI GCNGC 7 cut(s) 45, 163, 166, 897, 1089, 1143, 1275
Sau3AI GATC 9 cut(s) 567, 576, 583, 610, 655, 1240, 1392, 1481, 1512
Sau96I GGNCC 4 cut(s) 222, 779, 935, 1521
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 840, 1466
SfaNI GCATC 5 cut(s) 159, 193, 264, 274, 1331
SfcI CTRYAG 2 cut(s) 163, 342
SinI GGWCC 3 cut(s) 222, 779, 935
SmiMI CAYNNNNRTG 3 cut(s) 266, 516, 1068
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SnaBI TACGTA 1 cut(s) 1491
SphI GCATGC 1 cut(s) 903
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 4 cut(s) 11, 100, 560, 1142
SspI AATATT 1 cut(s) 1132
SspMI CTAG 1 cut(s) 534
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 2 cut(s) 838, 1464
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 7 cut(s) 343, 751, 962, 1046, 1180, 1369, 1454
TaiI ACGT 2 cut(s) 123, 1493
TaqI TCGA 2 cut(s) 84, 607
TatI WGTACW 1 cut(s) 870
TauI GCSGC 1 cut(s) 1145
TfiI GAWTC 4 cut(s) 214, 405, 734, 843
Tru1I TTAA 4 cut(s) 141, 338, 351, 522
Tru9I TTAA 4 cut(s) 141, 338, 351, 522
TscAI CASTG 3 cut(s) 926, 1051, 1225
TseI GCWGC 6 cut(s) 44, 162, 165, 896, 1088, 1274
TspDTI ATGAA 5 cut(s) 500, 697, 714, 1425, 1517
TspGWI ACGGA 4 cut(s) 104, 393, 1049, 1319
TspRI CASTG 3 cut(s) 926, 1051, 1225
VpaK11BI GGWCC 3 cut(s) 222, 779, 935
XapI RAATTY 4 cut(s) 255, 715, 728, 851
XceI RCATGY 4 cut(s) 497, 903, 1286, 1448
XmnI GAANNNNTTC 2 cut(s) 1005, 1538
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.