pycom11g18660

Endoglucanase 11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0004610
Physical Location & Seq
Forward (+)
20824697 .. 20827677
2981 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1578 bp
ATGAAGCAAGGTATTGTCCAATGGCTTTCTCTACTCTCCATTTTCGCACTCTTTTCCTTGTCTCAGACTCAGTCCTTCGACTACGGCGAAGCTCTCTCCAAGAGTCTCCTCTACTTCGAGTCCCAACGTTCCGGCCGCTTGCCCCACAACCAACGGGTCACCTGGCGTCACCATTCCGGCCTTGACGATGGCCTAGAACAAGGGGTGGACTTGGTGGGAGGGTACTATGATGCAGGTGACAATGTGAAATTTGGTCTGCCAATGGCTTTTACCATAACAATGCTGTCTTGGGGTGTAATTGAGTACGGCAAACAAATAGAGGATGCTGGAGAGTACAATCATGCCTTAGAAGCTATCAAATGGGGGACTGATTATTTCATCAAGGCTCATACTCACCCAAATGTTTTGTGGGCTGAGGTGGGAGATGGATCGACGGATCACTACTGTTGGCAAAGGCCGGAGGACATGACAACGTCTCGACAAGCCTACAAGGTCGATGAGAACCACCCCGGGTCAGATATTGCTGGTGAGACTGCGGCGGCAATGGCAGCGGCTGCTATTGTATTTAGGAAAACCAACCCACATTACTCTGGCCTACTTTTACACCATGCACAACAGTTGTTTGAGTTTGGTGACAAATATAGAGGTAAATATGATGAGAGTATTGGAGTGGTGAAGGGGCACTACACATCTTGGAGTGGTTACATGGATGAGTTGCTCTGGGCAGCTTTGTGGTTGTACAAAGCCACACAGAATGAGGATTATTTGAACTATGCTTTGAACAATGGCCAGTCTCTTGGGGGCACTACTTGGGCCATCAAAGAGTTTAGCTGGGATGTTAAGTATGCTGGTGTTCAAATCATTGCTTCAATGTTTGTTTGTTTCAATTCCTTAACTCTCACATACGTTTTCTTTTTTCAGTTGCTAAAGGAAGAGAAACACAAGCAACACATGCAAATACTGCAACAGTATCGTTCAAAAGCAGAGTTTTACATGTGTGCTTGCCTTGACAGAAACAACGTGGCCAACGTGCAACGCACCCCAGGAGGCTTGTTGTATATCCGCCAATGGAACAACATGCAATACGTCTCAAACGCAGCGTTTCTACTCACAACATACACTGATTATCTCCAAGCCACTCATGAGAATCTTACTTGCGACAAAGGGCAGGTGAGCCCACAAGAAATCTTATCTTTTGCCAAATCACAGGTTGATTACATTCTGGGGTCCAATCCAATGGCCATGAGCTACTTGGTTGGGTATGGACCAAAATACCCTCAAAGGGTGCACCACAGAGGAGCATCCATTGATTCGTATAAGAAGAACAAGGGTTTCATTGGGTGCACTCAGGGGTACGATAGCTGGTATGGGAAGCAGGGTCCAAACCCTAACGTGCTTGTTGGGGCACTGGTTGGTGGGCCCGATGGGAACGATGGATTCAGGGACGAGAGGTGGAATTACATGCAGACTGAGGCATGCACTTACAATACTGCAACATTAGTTGGGGTTCTTGCTAAATTGGATAGATTAAAAGGTGATTATATGTGGCTTAATCAATCATTAATAGCTTCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

526

Amino Acids

59.45

Weight (kDa)

6.1

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 504 1.1e-168 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 224, 1159
Acc36I ACCTGC 2 cut(s) 224, 1159
AciI CCGC 5 cut(s) 136, 536, 539, 551, 1063
AclI AACGTT 1 cut(s) 127
AclWI GGATC 2 cut(s) 436, 444
AcoI YGGCCR 4 cut(s) 133, 787, 1023, 1239
AcsI RAATTY 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 5 cut(s) 224, 305, 335, 740, 1355
AfiI CCNNNNNNNGG 1 cut(s) 1282
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 7 cut(s) 769, 781, 857, 870, 886, 978, 1572
AjnI CCWGG 2 cut(s) 161, 1042
AluBI AGCT 8 cut(s) 92, 353, 728, 831, 1248, 1362, 1568, 1575
AluI AGCT 8 cut(s) 92, 353, 728, 831, 1248, 1362, 1568, 1575
Alw21I GWGCWC 2 cut(s) 1290, 1346
Alw26I GTCTC 6 cut(s) 66, 110, 480, 524, 798, 1093
Alw44I GTGCAC 2 cut(s) 1286, 1342
AlwI GGATC 2 cut(s) 436, 444
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 509
ApaI GGGCCC 1 cut(s) 1422
ApaLI GTGCAC 2 cut(s) 1286, 1342
ApeKI GCWGC 4 cut(s) 548, 554, 725, 1097
ApoI RAATTY 1 cut(s) 248
AseI ATTAAT 1 cut(s) 1562
AspS9I GGNCC 6 cut(s) 813, 1227, 1265, 1379, 1418, 1419
AsuC2I CCSGG 2 cut(s) 510, 511
AsuHPI GGTGA 9 cut(s) 151, 161, 248, 386, 539, 644, 685, 1183, 1547
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 3 cut(s) 1227, 1265, 1379
BaeGI GKGCMC 6 cut(s) 684, 806, 1290, 1346, 1408, 1422
BalI TGGCCA 3 cut(s) 789, 1025, 1241
BanII GRGCYC 2 cut(s) 1178, 1422
BarI GAAGNNNNNNTAC 2 cut(s) 668, 700
Bbv12I GWGCWC 2 cut(s) 1290, 1346
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 4 cut(s) 541, 560, 737, 1109
BccI CCATC 5 cut(s) 182, 419, 824, 1418, 1427
BceAI ACGGC 2 cut(s) 100, 322
BcgI CGANNNNNNTGC 2 cut(s) 953, 987
BciT130I CCWGG 2 cut(s) 163, 1044
BcnI CCSGG 2 cut(s) 510, 511
BcoDI GTCTC 6 cut(s) 66, 110, 480, 524, 798, 1093
BfaI CTAG 2 cut(s) 194, 1576
BfuAI ACCTGC 2 cut(s) 224, 1159
BisI GCNGC 8 cut(s) 136, 537, 540, 549, 552, 555, 726, 1098
BlsI GCNGC 8 cut(s) 137, 538, 541, 550, 553, 556, 727, 1099
Bme1390I CCNGG 4 cut(s) 163, 510, 511, 1044
Bme18I GGWCC 3 cut(s) 1227, 1265, 1379
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 6 cut(s) 813, 1227, 1265, 1379, 1418, 1419
BmiI GGNNCC 3 cut(s) 1228, 1380, 1420
BmrFI CCNGG 4 cut(s) 163, 510, 511, 1044
BmsI GCATC 3 cut(s) 220, 313, 1310
BpmI CTGGAG 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 414
BpuMI CCSGG 2 cut(s) 510, 511
BsaHI GRCGYC 1 cut(s) 166
BsaJI CCNNGG 3 cut(s) 508, 509, 1042
Bsc4I CCNNNNNNNGG 1 cut(s) 1282
Bse1I ACTGG 2 cut(s) 790, 1413
Bse3DI GCAATG 2 cut(s) 549, 861
BseBI CCWGG 2 cut(s) 163, 1044
BseDI CCNNGG 3 cut(s) 508, 509, 1042
BseGI GGATG 4 cut(s) 328, 715, 841, 1301
BseLI CCNNNNNNNGG 1 cut(s) 1282
BseMI GCAATG 2 cut(s) 549, 861
BseMII CTCAG 5 cut(s) 77, 83, 405, 1361, 1461
BseNI ACTGG 2 cut(s) 790, 1413
BseRI GAGGAG 2 cut(s) 98, 1311
BseSI GKGCMC 6 cut(s) 684, 806, 1290, 1346, 1408, 1422
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 4 cut(s) 541, 560, 737, 1109
BseYI CCCAGC 1 cut(s) 831
Bsh1285I CGRYCG 1 cut(s) 136
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 2 cut(s) 1290, 1346
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 4 cut(s) 132, 177, 458, 510
BslFI GGGAC 3 cut(s) 106, 379, 1457
BslI CCNNNNNNNGG 1 cut(s) 1282
BsmAI GTCTC 6 cut(s) 66, 110, 480, 524, 798, 1093
BsmBI CGTCTC 2 cut(s) 480, 1093
BsmFI GGGAC 3 cut(s) 106, 379, 1457
BsoBI CYCGRG 1 cut(s) 509
Bsp120I GGGCCC 1 cut(s) 1418
Bsp1286I GDGCHC 7 cut(s) 684, 806, 1178, 1290, 1346, 1408, 1422
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 2 cut(s) 428, 436
BspACI CCGC 5 cut(s) 136, 536, 539, 551, 1063
BspCNI CTCAG 5 cut(s) 76, 82, 406, 1360, 1462
BspHI TCATGA 1 cut(s) 1141
BspLI GGNNCC 3 cut(s) 1228, 1380, 1420
BspMI ACCTGC 2 cut(s) 224, 1159
BspPI GGATC 2 cut(s) 436, 444
BsrDI GCAATG 2 cut(s) 549, 861
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 2 cut(s) 790, 1413
BssECI CCNNGG 3 cut(s) 508, 509, 1042
BssMI GATC 2 cut(s) 428, 436
BssNI GRCGYC 1 cut(s) 166
Bst2UI CCWGG 2 cut(s) 163, 1044
Bst4CI ACNGT 3 cut(s) 446, 618, 969
Bst6I CTCTTC 1 cut(s) 927
BstACI GRCGYC 1 cut(s) 166
BstAPI GCANNNNNTGC 3 cut(s) 554, 952, 961
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 3 cut(s) 140, 1003, 1477
BstDEI CTNAG 6 cut(s) 63, 69, 346, 414, 1347, 1470
BstEII GGTNACC 1 cut(s) 157
BstF5I GGATG 4 cut(s) 328, 715, 841, 1301
BstKTI GATC 2 cut(s) 431, 439
BstMAI GTCTC 6 cut(s) 66, 110, 480, 524, 798, 1093
BstMBI GATC 2 cut(s) 428, 436
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 6 cut(s) 350, 545, 548, 554, 952, 961
BstNI CCWGG 2 cut(s) 163, 1044
BstNSI RCATGY 5 cut(s) 955, 997, 1081, 1465, 1479
BstPI GGTNACC 1 cut(s) 157
BstSCI CCNGG 4 cut(s) 161, 508, 509, 1042
BstSLI GKGCMC 6 cut(s) 684, 806, 1290, 1346, 1408, 1422
BstV1I GCAGC 4 cut(s) 541, 560, 737, 1109
BstXI CCANNNNNNTGG 2 cut(s) 797, 1237
BstZI CGGCCG 1 cut(s) 133
BtsCI GGATG 4 cut(s) 328, 715, 841, 1301
BtsIMutI CAGTG 2 cut(s) 1119, 1406
BveI ACCTGC 2 cut(s) 224, 1159
Cac8I GCNNGC 3 cut(s) 140, 1003, 1477
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 1 cut(s) 1141
Cfr13I GGNCC 6 cut(s) 813, 1227, 1265, 1379, 1418, 1419
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 1 cut(s) 155
Csp6I GTAC 5 cut(s) 223, 304, 334, 739, 1354
CviQI GTAC 5 cut(s) 223, 304, 334, 739, 1354
DdeI CTNAG 6 cut(s) 63, 69, 346, 414, 1347, 1470
DpnI GATC 2 cut(s) 430, 438
DpnII GATC 2 cut(s) 428, 436
EaeI YGGCCR 4 cut(s) 133, 787, 1023, 1239
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
EciI GGCGGA 1 cut(s) 1052
EclXI CGGCCG 1 cut(s) 133
Eco24I GRGCYC 2 cut(s) 1178, 1422
Eco47I GGWCC 3 cut(s) 1227, 1265, 1379
Eco52I CGGCCG 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 157
EcoO65I GGTNACC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 161, 1042
EcoT38I GRGCYC 2 cut(s) 1178, 1422
Esp3I CGTCTC 2 cut(s) 480, 1093
FaqI GGGAC 3 cut(s) 106, 379, 1457
Fnu4HI GCNGC 8 cut(s) 136, 537, 540, 549, 552, 555, 726, 1098
FokI GGATG 4 cut(s) 335, 722, 848, 1288
FriOI GRGCYC 2 cut(s) 1178, 1422
Fsp4HI GCNGC 8 cut(s) 136, 537, 540, 549, 552, 555, 726, 1098
FspBI CTAG 2 cut(s) 194, 1576
GluI GCNGC 8 cut(s) 136, 537, 540, 549, 552, 555, 726, 1098
GsaI CCCAGC 1 cut(s) 835
GsuI CTGGAG 1 cut(s) 348
HapII CCGG 4 cut(s) 132, 177, 458, 510
HgaI GACGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 166
HinfI GANTC 6 cut(s) 67, 103, 119, 1147, 1310, 1437
HpaII CCGG 4 cut(s) 132, 177, 458, 510
HphI GGTGA 9 cut(s) 151, 161, 248, 386, 539, 644, 685, 1183, 1547
Hpy166II GTNNAC 3 cut(s) 208, 1288, 1344
Hpy188I TCNGA 2 cut(s) 66, 517
Hpy188III TCNNGA 2 cut(s) 477, 1142
Hpy8I GTNNAC 3 cut(s) 208, 1288, 1344
Hpy99I CGWCG 1 cut(s) 436
HpyAV CCTTC 2 cut(s) 85, 670
HpyCH4III ACNGT 3 cut(s) 446, 618, 969
HpyCH4IV ACGT 7 cut(s) 127, 473, 906, 1020, 1029, 1086, 1392
HpyF10VI GCNNNNNNNGC 6 cut(s) 350, 545, 548, 554, 952, 961
HpyF3I CTNAG 6 cut(s) 63, 69, 346, 414, 1347, 1470
HpySE526I ACGT 7 cut(s) 127, 473, 906, 1020, 1029, 1086, 1392
Hsp92I GRCGYC 1 cut(s) 166
Kzo9I GATC 2 cut(s) 428, 436
LmnI GCTCC 1 cut(s) 1298
Lsp1109I GCAGC 4 cut(s) 541, 560, 737, 1109
LweI GCATC 3 cut(s) 220, 313, 1310
MaeI CTAG 2 cut(s) 194, 1576
MaeII ACGT 7 cut(s) 127, 473, 906, 1020, 1029, 1086, 1392
MaeIII GTNAC 5 cut(s) 157, 167, 236, 632, 701
MalI GATC 2 cut(s) 430, 438
MboI GATC 2 cut(s) 428, 436
MboII GAAGA 2 cut(s) 944, 1333
MhlI GDGCHC 7 cut(s) 684, 806, 1178, 1290, 1346, 1408, 1422
MlsI TGGCCA 3 cut(s) 789, 1025, 1241
MluCI AATT 5 cut(s) 248, 297, 886, 1456, 1517
MluNI TGGCCA 3 cut(s) 789, 1025, 1241
MlyI GAGTC 3 cut(s) 61, 112, 128
Mox20I TGGCCA 3 cut(s) 789, 1025, 1241
MscI TGGCCA 3 cut(s) 789, 1025, 1241
MseI TTAA 5 cut(s) 840, 893, 1529, 1551, 1562
MslI CAYNNNNRTG 2 cut(s) 278, 399
Msp20I TGGCCA 3 cut(s) 789, 1025, 1241
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 4 cut(s) 132, 177, 458, 510
MspR9I CCNGG 4 cut(s) 163, 510, 511, 1044
MvaI CCWGG 2 cut(s) 163, 1044
MwoI GCNNNNNNNGC 6 cut(s) 350, 545, 548, 554, 952, 961
NciI CCSGG 2 cut(s) 510, 511
NdeII GATC 2 cut(s) 428, 436
NlaIV GGNNCC 3 cut(s) 1228, 1380, 1420
NmuCI GTSAC 4 cut(s) 157, 167, 236, 632
NspI RCATGY 5 cut(s) 955, 997, 1081, 1465, 1479
PaeI GCATGC 1 cut(s) 1479
PagI TCATGA 1 cut(s) 1141
PaqCI CACCTGC 2 cut(s) 224, 1159
PciI ACATGT 1 cut(s) 993
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 1026
PfeI GAWTC 3 cut(s) 1147, 1310, 1437
PflFI GACNNNGTC 2 cut(s) 70, 472
PkrI GCNGC 8 cut(s) 137, 538, 541, 550, 553, 556, 727, 1099
PleI GAGTC 3 cut(s) 61, 111, 127
PpsI GAGTC 3 cut(s) 61, 111, 127
PscI ACATGT 1 cut(s) 993
PshBI ATTAAT 1 cut(s) 1562
Psp1406I AACGTT 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 161, 1042
PspEI GGTNACC 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 831
PspGI CCWGG 2 cut(s) 161, 1042
PspN4I GGNNCC 3 cut(s) 1228, 1380, 1420
PspOMI GGGCCC 1 cut(s) 1418
PspPI GGNCC 6 cut(s) 813, 1227, 1265, 1379, 1418, 1419
PstNI CAGNNNCTG 1 cut(s) 554
PsyI GACNNNGTC 2 cut(s) 70, 472
RsaI GTAC 5 cut(s) 224, 305, 335, 740, 1355
RsaNI GTAC 5 cut(s) 223, 304, 334, 739, 1354
RseI CAYNNNNRTG 2 cut(s) 278, 399
SaqAI TTAA 5 cut(s) 840, 893, 1529, 1551, 1562
SatI GCNGC 8 cut(s) 136, 537, 540, 549, 552, 555, 726, 1098
Sau3AI GATC 2 cut(s) 428, 436
Sau96I GGNCC 6 cut(s) 813, 1227, 1265, 1379, 1418, 1419
SchI GAGTC 3 cut(s) 61, 112, 128
ScrFI CCNGG 4 cut(s) 163, 510, 511, 1044
SduI GDGCHC 7 cut(s) 684, 806, 1178, 1290, 1346, 1408, 1422
SfaNI GCATC 3 cut(s) 220, 313, 1310
SinI GGWCC 3 cut(s) 1227, 1265, 1379
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 2 cut(s) 278, 399
SphI GCATGC 1 cut(s) 1479
Sse9I AATT 5 cut(s) 248, 297, 886, 1456, 1517
SsiI CCGC 5 cut(s) 136, 536, 539, 551, 1063
SspMI CTAG 2 cut(s) 194, 1576
StyD4I CCNGG 4 cut(s) 161, 508, 509, 1042
TaaI ACNGT 3 cut(s) 446, 618, 969
TaiI ACGT 7 cut(s) 130, 476, 909, 1023, 1032, 1089, 1395
TaqI TCGA 5 cut(s) 78, 117, 431, 478, 495
TasI AATT 5 cut(s) 248, 297, 886, 1456, 1517
TatI WGTACW 2 cut(s) 333, 738
TauI GCSGC 4 cut(s) 138, 539, 542, 554
TfiI GAWTC 3 cut(s) 1147, 1310, 1437
Tru1I TTAA 5 cut(s) 840, 893, 1529, 1551, 1562
Tru9I TTAA 5 cut(s) 840, 893, 1529, 1551, 1562
TscAI CASTG 2 cut(s) 1126, 1413
TseFI GTSAC 4 cut(s) 157, 167, 236, 632
TseI GCWGC 4 cut(s) 548, 554, 725, 1097
Tsp45I GTSAC 4 cut(s) 157, 167, 236, 632
TspDTI ATGAA 3 cut(s) 17, 367, 1324
TspGWI ACGGA 1 cut(s) 449
TspMI CCCGGG 1 cut(s) 509
TspRI CASTG 2 cut(s) 1126, 1413
Tth111I GACNNNGTC 2 cut(s) 70, 472
VneI GTGCAC 2 cut(s) 1286, 1342
VpaK11BI GGWCC 3 cut(s) 1227, 1265, 1379
VspI ATTAAT 1 cut(s) 1562
XapI RAATTY 1 cut(s) 248
XceI RCATGY 5 cut(s) 955, 997, 1081, 1465, 1479
XcmI CCANNNNNNNNNTGG 2 cut(s) 405, 1249
XmaI CCCGGG 1 cut(s) 509
XspI CTAG 2 cut(s) 194, 1576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.