pycom11g19160

Ribonuclease UK114-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006616
Physical Location & Seq
Forward (+)
21426678 .. 21428732
2055 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGGCATGGTGTGCGGCGAGGAGCTTTCACATGCCGGCGCTCGACATGGCCGCATTGCGCACCCGGGCTCCGTTAGCCGCCGGCGCGGGCTTTGCCTCGGTTGCCGGTTCCACTCTCTGGCGATCCTTGGCTTCATCGAAGCCCTCGTCTCGCTTTGCTTGCTTAGCCATTTCCACCGATGCTCGAATTAAGGAAGCTGTTCACACAGACAAGGCTCCGGCTGCATTGGGACCGTATTCTCAGGCAATCAAGGCCAACAACTTTCTCCATGTGTCGGGTTGTCTTGGTCTGATTCCTGAGACAGGGAAGTTTGTGTCGGATAATGTTGAAGATCAGACAGAGCAGGTTCTAAAAAATATGGGGGAAATACTGAAAGCAAGCGGGGCCAGCTATTCTTCTGTGGTTAAAACGACTATTCTGTTGGCTGATTTGAAAGACTTCAAGAAAGTCAATGAAATCTATGCTAAATATTTTCCATCTCCAGCCCCAGCACGTTCAACATTTCAGGTAGCGGCGCTGCCATTGGATGCCAAGATTGAAATTGAGTGCATTGCAGCACTGTAA

Protein Analysis

188

Amino Acids

19.83

Weight (kDa)

8.9

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuc_L-PSP PF01042 70 - 186 4.7e-42 Endoribonuclease L-PSP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 334
AccB7I CCANNNNNTGG 1 cut(s) 117
AccII CGCG 1 cut(s) 86
AciI CCGC 6 cut(s) 14, 51, 78, 86, 381, 512
AclWI GGATC 1 cut(s) 117
AcoI YGGCCR 1 cut(s) 48
AfiI CCNNNNNNNGG 3 cut(s) 117, 274, 302
AgsI TTSAA 5 cut(s) 329, 433, 442, 498, 539
AluBI AGCT 3 cut(s) 24, 197, 390
AluI AGCT 3 cut(s) 24, 197, 390
Alw26I GTCTC 2 cut(s) 153, 293
AlwI GGATC 1 cut(s) 117
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 3 cut(s) 48, 252, 384
ApeKI GCWGC 3 cut(s) 221, 517, 554
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
Asp700I GAANNNNTTC 2 cut(s) 198, 437
AspLEI GCGC 4 cut(s) 40, 60, 86, 517
AspS9I GGNCC 2 cut(s) 230, 384
AsuC2I CCSGG 2 cut(s) 64, 65
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 208, 504
BccI CCATC 1 cut(s) 484
BcnI CCSGG 2 cut(s) 64, 65
BcoDI GTCTC 2 cut(s) 153, 293
BfoI RGCGCY 2 cut(s) 41, 518
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 7 cut(s) 15, 51, 78, 222, 513, 518, 555
BlpI GCTNAGC 1 cut(s) 163
BlsI GCNGC 7 cut(s) 16, 52, 79, 223, 514, 519, 556
Bme1390I CCNGG 2 cut(s) 64, 65
Bme18I GGWCC 1 cut(s) 230
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 230, 384
BmiI GGNNCC 5 cut(s) 69, 109, 216, 231, 385
BmrFI CCNGG 2 cut(s) 64, 65
BmsI GCATC 2 cut(s) 169, 517
BpmI CTGGAG 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 163
BpuMI CCSGG 2 cut(s) 64, 65
BsaJI CCNNGG 3 cut(s) 63, 96, 126
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 274, 302
Bse118I RCCGGY 3 cut(s) 34, 80, 104
Bse3DI GCAATG 2 cut(s) 53, 549
BseDI CCNNGG 3 cut(s) 63, 96, 126
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 3 cut(s) 117, 274, 302
BseMI GCAATG 2 cut(s) 53, 549
BseMII CTCAG 2 cut(s) 254, 288
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 2 cut(s) 208, 504
BseYI CCCAGC 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 3 cut(s) 50, 254, 386
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 5 cut(s) 35, 64, 81, 105, 218
BslFI GGGAC 1 cut(s) 243
BslI CCNNNNNNNGG 3 cut(s) 117, 274, 302
BsmAI GTCTC 2 cut(s) 153, 293
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 243
BsnI GGCC 3 cut(s) 50, 254, 386
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 2 cut(s) 122, 331
Bsp1720I GCTNAGC 1 cut(s) 163
BspACI CCGC 6 cut(s) 14, 51, 78, 86, 381, 512
BspANI GGCC 3 cut(s) 50, 254, 386
BspCNI CTCAG 2 cut(s) 253, 289
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 5 cut(s) 69, 109, 216, 231, 385
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 53, 549
BsrFI RCCGGY 3 cut(s) 34, 80, 104
BssAI RCCGGY 3 cut(s) 34, 80, 104
BssECI CCNNGG 3 cut(s) 63, 96, 126
BssMI GATC 2 cut(s) 122, 331
BssT1I CCWWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 234, 561
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 6 cut(s) 36, 82, 88, 160, 379, 388
BstDEI CTNAG 3 cut(s) 163, 240, 297
BstENI CCTNNNNNAGG 1 cut(s) 300
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 1 cut(s) 86
BstH2I RGCGCY 2 cut(s) 41, 518
BstHHI GCGC 4 cut(s) 40, 60, 86, 517
BstKTI GATC 2 cut(s) 125, 334
BstMAI GTCTC 2 cut(s) 153, 293
BstMBI GATC 2 cut(s) 122, 331
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 62, 63
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 2 cut(s) 208, 504
BsuRI GGCC 3 cut(s) 50, 254, 386
BtsCI GGATG 1 cut(s) 532
BtsIMutI CAGTG 1 cut(s) 557
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 6 cut(s) 36, 82, 88, 160, 379, 388
CfoI GCGC 4 cut(s) 40, 60, 86, 517
Cfr10I RCCGGY 3 cut(s) 34, 80, 104
Cfr13I GGNCC 2 cut(s) 230, 384
Cfr9I CCCGGG 1 cut(s) 63
CviAII CATG 4 cut(s) 6, 31, 46, 269
DdeI CTNAG 3 cut(s) 163, 240, 297
DpnI GATC 2 cut(s) 124, 333
DpnII GATC 2 cut(s) 122, 331
EaeI YGGCCR 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 63
EcoNI CCTNNNNNAGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 126
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 4 cut(s) 9, 34, 49, 272
FaiI YATR 6 cut(s) 7, 32, 47, 270, 359, 462
FaqI GGGAC 1 cut(s) 243
FatI CATG 4 cut(s) 5, 30, 45, 268
FauI CCCGC 2 cut(s) 79, 374
Fnu4HI GCNGC 7 cut(s) 15, 51, 78, 222, 513, 518, 555
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 7 cut(s) 15, 51, 78, 222, 513, 518, 555
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 4 cut(s) 39, 59, 85, 516
GluI GCNGC 7 cut(s) 15, 51, 78, 222, 513, 518, 555
GsaI CCCAGC 1 cut(s) 491
GsuI CTGGAG 1 cut(s) 465
HaeII RGCGCY 2 cut(s) 41, 518
HaeIII GGCC 3 cut(s) 50, 254, 386
HapII CCGG 5 cut(s) 35, 64, 81, 105, 218
HhaI GCGC 4 cut(s) 40, 60, 86, 517
Hin1II CATG 4 cut(s) 9, 34, 49, 272
Hin6I GCGC 4 cut(s) 38, 58, 84, 515
HinP1I GCGC 4 cut(s) 38, 58, 84, 515
HinfI GANTC 1 cut(s) 292
HpaII CCGG 5 cut(s) 35, 64, 81, 105, 218
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 3 cut(s) 291, 319, 336
Hpy188III TCNNGA 2 cut(s) 296, 442
Hpy8I GTNNAC 1 cut(s) 202
HpyCH4III ACNGT 2 cut(s) 234, 561
HpyCH4IV ACGT 1 cut(s) 493
HpyCH4V TGCA 3 cut(s) 224, 549, 554
HpyF3I CTNAG 3 cut(s) 163, 240, 297
HpySE526I ACGT 1 cut(s) 493
Hsp92II CATG 4 cut(s) 9, 34, 49, 272
HspAI GCGC 4 cut(s) 38, 58, 84, 515
KroI GCCGGC 2 cut(s) 34, 80
KroNI GCCGGC 2 cut(s) 36, 82
Kzo9I GATC 2 cut(s) 122, 331
LmnI GCTCC 3 cut(s) 21, 73, 220
Lsp1109I GCAGC 2 cut(s) 208, 504
LweI GCATC 2 cut(s) 169, 517
MaeII ACGT 1 cut(s) 493
MalI GATC 2 cut(s) 124, 333
MboI GATC 2 cut(s) 122, 331
MboII GAAGA 2 cut(s) 341, 387
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 2 cut(s) 186, 540
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 3 cut(s) 12, 106, 154
MreI CGCCGGCG 1 cut(s) 80
MroNI GCCGGC 2 cut(s) 34, 80
MroXI GAANNNNTTC 2 cut(s) 198, 437
MseI TTAA 2 cut(s) 189, 405
MspI CCGG 5 cut(s) 35, 64, 81, 105, 218
MspR9I CCNGG 2 cut(s) 64, 65
MvnI CGCG 1 cut(s) 86
NaeI GCCGGC 2 cut(s) 36, 82
NciI CCSGG 2 cut(s) 64, 65
NdeII GATC 2 cut(s) 122, 331
NgoMIV GCCGGC 2 cut(s) 34, 80
NlaIII CATG 4 cut(s) 9, 34, 49, 272
NlaIV GGNNCC 5 cut(s) 69, 109, 216, 231, 385
NsbI TGCGCA 1 cut(s) 59
NspI RCATGY 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PdiI GCCGGC 2 cut(s) 36, 82
PdmI GAANNNNTTC 2 cut(s) 198, 437
PfeI GAWTC 1 cut(s) 292
PflMI CCANNNNNTGG 1 cut(s) 117
PkrI GCNGC 7 cut(s) 16, 52, 79, 223, 514, 519, 556
PspFI CCCAGC 1 cut(s) 487
PspN4I GGNNCC 5 cut(s) 69, 109, 216, 231, 385
PspPI GGNCC 2 cut(s) 230, 384
SaqAI TTAA 2 cut(s) 189, 405
SatI GCNGC 7 cut(s) 15, 51, 78, 222, 513, 518, 555
Sau3AI GATC 2 cut(s) 122, 331
Sau96I GGNCC 2 cut(s) 230, 384
ScrFI CCNGG 2 cut(s) 64, 65
SduI GDGCHC 1 cut(s) 70
SetI ASST 6 cut(s) 26, 199, 348, 392, 496, 510
SfaNI GCATC 2 cut(s) 169, 517
SgrAI CRCCGGYG 1 cut(s) 80
SinI GGWCC 1 cut(s) 230
SmaI CCCGGG 1 cut(s) 65
Sse9I AATT 2 cut(s) 186, 540
SsiI CCGC 6 cut(s) 14, 51, 78, 86, 381, 512
SspI AATATT 1 cut(s) 470
StyD4I CCNGG 2 cut(s) 62, 63
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 234, 561
TaiI ACGT 1 cut(s) 496
TaqI TCGA 3 cut(s) 42, 137, 184
TasI AATT 2 cut(s) 186, 540
TauI GCSGC 4 cut(s) 17, 53, 80, 515
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 2 cut(s) 189, 405
Tru9I TTAA 2 cut(s) 189, 405
TscAI CASTG 1 cut(s) 564
TseI GCWGC 3 cut(s) 221, 517, 554
TspDTI ATGAA 2 cut(s) 123, 468
TspGWI ACGGA 1 cut(s) 60
TspMI CCCGGG 1 cut(s) 63
TspRI CASTG 1 cut(s) 564
Van91I CCANNNNNTGG 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 230
XagI CCTNNNNNAGG 1 cut(s) 300
XceI RCATGY 1 cut(s) 34
XmaI CCCGGG 1 cut(s) 63
XmnI GAANNNNTTC 2 cut(s) 198, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.