Rh5CG303900

Ribonuclease UK114-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0006616
Physical Location & Seq
Reverse (-)
33459547 .. 33462152
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG303900.1

Sequence Viewer

Length: 447 bp
ATGGCGTGGTCTGCGGCGAGGAGCTTCCACATGCCGACGATCGACATGGGCGCACTGCGCACCGGGGCTCCCTTAGCCGTCGCCGGCGTCTGCTGTGCCTCGGTAGCCGGGTGGCGATCCTTGGTTTCATCGAAGCCCTCTTCCCGTTTCGCTTGCTTAGCAATTTCCACTGACGCTGGAATCAAGGAGGCTGTGAAAACAGATAAGGCTCCGGCTGCATTGGGACCCTATTCTCAGGCAATCAAAGCTAACAATGTCCTCTATGTGTCTGGTTGTCTTGGTCTGATTCCGGAGACCGGAAAGTTCATCTCGGATGATGTTGAAGGTCAGACGGAGCAGGTTCTCAAGAATATGGGAGAAATACTGAAAGCAAGTGGTGCTGATTATTCGTCTGTGGTTAAAACAACTATTTTGTATGGCTTCTTTGTCCCTTTGCTCAAATCTTGA

Protein Analysis

148

Amino Acids

15.43

Weight (kDa)

8.7

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuc_L-PSP PF01042 68 - 138 2.7e-21 Endoribonuclease L-PSP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 328
AccIII TCCGGA 1 cut(s) 289
AciI CCGC 1 cut(s) 14
AclWI GGATC 1 cut(s) 111
AcyI GRCGYC 1 cut(s) 87
AfiI CCNNNNNNNGG 1 cut(s) 296
AgsI TTSAA 1 cut(s) 323
AluBI AGCT 2 cut(s) 24, 248
AluI AGCT 2 cut(s) 24, 248
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 1 cut(s) 111
Aor13HI TCCGGA 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 53, 60
AspS9I GGNCC 1 cut(s) 224
AsuC2I CCSGG 2 cut(s) 64, 109
AvaII GGWCC 1 cut(s) 224
BanII GRGCYC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 202
BceAI ACGGC 1 cut(s) 62
BcnI CCSGG 2 cut(s) 64, 109
BcoDI GTCTC 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 2 cut(s) 15, 216
BlpI GCTNAGC 1 cut(s) 157
BlsI GCNGC 2 cut(s) 16, 217
Bme1390I CCNGG 2 cut(s) 64, 109
Bme18I GGWCC 1 cut(s) 224
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 4 cut(s) 69, 210, 225, 226
BmrFI CCNGG 2 cut(s) 64, 109
Bpu10I CCTNAGC 1 cut(s) 73
Bpu1102I GCTNAGC 1 cut(s) 157
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 2 cut(s) 64, 109
BsaHI GRCGYC 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 3 cut(s) 63, 99, 120
BsaWI WCCGGW 2 cut(s) 289, 296
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse118I RCCGGY 1 cut(s) 83
BseAI TCCGGA 1 cut(s) 289
BseDI CCNNGG 3 cut(s) 63, 99, 120
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 248
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 1 cut(s) 202
Bsh1285I CGRYCG 1 cut(s) 42
BsiEI CGRYCG 1 cut(s) 42
BsiSI CCGG 6 cut(s) 63, 84, 108, 212, 290, 297
BslFI GGGAC 2 cut(s) 237, 413
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 2 cut(s) 237, 413
Bso31I GGTCTC 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 70
Bsp13I TCCGGA 1 cut(s) 289
Bsp143I GATC 2 cut(s) 39, 116
Bsp1720I GCTNAGC 1 cut(s) 157
BspACI CCGC 1 cut(s) 14
BspCNI CTCAG 1 cut(s) 247
BspEI TCCGGA 1 cut(s) 289
BspLI GGNNCC 4 cut(s) 69, 210, 225, 226
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 83
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 3 cut(s) 63, 99, 120
BssMI GATC 2 cut(s) 39, 116
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 120
Bst6I CTCTTC 1 cut(s) 145
BstACI GRCGYC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 377
BstC8I GCNNGC 2 cut(s) 85, 154
BstDEI CTNAG 3 cut(s) 73, 157, 234
BstF5I GGATG 1 cut(s) 319
BstHHI GCGC 2 cut(s) 53, 60
BstKTI GATC 2 cut(s) 42, 119
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 2 cut(s) 39, 116
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 8 cut(s) 11, 57, 74, 104, 158, 215, 245, 377
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 62, 107
BstV1I GCAGC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 2 cut(s) 53, 168
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 85, 154
CfoI GCGC 2 cut(s) 53, 60
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 224
CseI GACGC 2 cut(s) 76, 182
CviAII CATG 2 cut(s) 31, 46
DdeI CTNAG 3 cut(s) 73, 157, 234
DpnI GATC 2 cut(s) 41, 118
DpnII GATC 2 cut(s) 39, 116
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 2 cut(s) 34, 49
FaiI YATR 5 cut(s) 32, 47, 264, 353, 417
FaqI GGGAC 2 cut(s) 237, 413
FatI CATG 2 cut(s) 30, 45
Fnu4HI GCNGC 2 cut(s) 15, 216
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 2 cut(s) 15, 216
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 2 cut(s) 52, 59
GluI GCNGC 2 cut(s) 15, 216
HapII CCGG 6 cut(s) 63, 84, 108, 212, 290, 297
HgaI GACGC 2 cut(s) 76, 182
HhaI GCGC 2 cut(s) 53, 60
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 2 cut(s) 34, 49
Hin6I GCGC 2 cut(s) 51, 58
HinP1I GCGC 2 cut(s) 51, 58
HinfI GANTC 2 cut(s) 180, 286
HpaII CCGG 6 cut(s) 63, 84, 108, 212, 290, 297
Hpy188I TCNGA 3 cut(s) 285, 313, 330
Hpy188III TCNNGA 3 cut(s) 290, 346, 444
Hpy99I CGWCG 2 cut(s) 40, 83
HpyAV CCTTC 1 cut(s) 317
HpyCH4V TGCA 1 cut(s) 218
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 57, 74, 104, 158, 215, 245, 377
HpyF3I CTNAG 3 cut(s) 73, 157, 234
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 2 cut(s) 34, 49
HspAI GCGC 2 cut(s) 51, 58
KflI GGGWCCC 1 cut(s) 224
Kpn2I TCCGGA 1 cut(s) 289
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Kzo9I GATC 2 cut(s) 39, 116
LmnI GCTCC 4 cut(s) 21, 73, 214, 334
Lsp1109I GCAGC 1 cut(s) 202
MalI GATC 2 cut(s) 41, 118
MboI GATC 2 cut(s) 39, 116
MboII GAAGA 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 1 cut(s) 162
MnlI CCTC 5 cut(s) 12, 109, 148, 181, 269
MreI CGCCGGCG 1 cut(s) 83
MroI TCCGGA 1 cut(s) 289
MroNI GCCGGC 1 cut(s) 83
MseI TTAA 1 cut(s) 399
MspI CCGG 6 cut(s) 63, 84, 108, 212, 290, 297
MspR9I CCNGG 2 cut(s) 64, 109
MwoI GCNNNNNNNGC 8 cut(s) 11, 57, 74, 104, 158, 215, 245, 377
NaeI GCCGGC 1 cut(s) 85
NciI CCSGG 2 cut(s) 64, 109
NdeII GATC 2 cut(s) 39, 116
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 2 cut(s) 34, 49
NlaIV GGNNCC 4 cut(s) 69, 210, 225, 226
NsbI TGCGCA 1 cut(s) 59
NspI RCATGY 1 cut(s) 34
PdiI GCCGGC 1 cut(s) 85
PfeI GAWTC 2 cut(s) 180, 286
PkrI GCNGC 2 cut(s) 16, 217
Ple19I CGATCG 1 cut(s) 42
PpuMI RGGWCCY 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 224
PspN4I GGNNCC 4 cut(s) 69, 210, 225, 226
PspPI GGNCC 1 cut(s) 224
PspPPI RGGWCCY 1 cut(s) 224
PvuI CGATCG 1 cut(s) 42
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 2 cut(s) 15, 216
Sau3AI GATC 2 cut(s) 39, 116
Sau96I GGNCC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 64, 109
SduI GDGCHC 1 cut(s) 70
SetI ASST 4 cut(s) 26, 250, 328, 342
SgrAI CRCCGGYG 1 cut(s) 83
SinI GGWCC 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 1 cut(s) 162
SsiI CCGC 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 62, 107
StyI CCWWGG 1 cut(s) 120
TaqI TCGA 2 cut(s) 42, 131
TasI AATT 1 cut(s) 162
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 2 cut(s) 180, 286
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 2 cut(s) 60, 175
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 2 cut(s) 117, 295
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 2 cut(s) 60, 175
VpaK11BI GGWCC 1 cut(s) 224
XceI RCATGY 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.