pycom11g20310

flavanone 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0013441
Physical Location & Seq
Forward (+)
23223448 .. 23226699
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
ATGATTACAACATCCAATACTATGATTAATTCAATATATATGTGCGTGTGTGTATATATAGCAGCCTCGGTGGAGGGCAAAAGAAGGGGAGCTATGGCAACATACTGTGTTACCGGAGCTACTGGTTTTATAGGGTCTTGGCTGGTGAAGACCCTCCTGGAGAAAGGTCATATAGTTCATGCAACAGTTCGTGATCCTGCCAAGTGTCTGCAGCTATTATCATTGTGGACAAGCACTGATCGGTTGAGATTGTTCCGAGCTGATTTGCGACAAGAAGGAAGCTTCGACGAGGCTGTGAAGGGCTGTGACGGCGTATTTCACGTTGCAGCATCAATGGAATTCAATGTTACTGTAACAGAAAACATTGAGAGGTATGTTCAATCAAATATCATTGAGCCTGCAATCAAAGGAACCTTAGACCTCCTCCAATCCTGTGTGAATTCTGGAACGGTGAAAAGGGTTGTACTCACCTCTTCCATTAGTACTATCACTGCCAAAGATGGCAACGGAAACGCAAGAGCTGTTGTTGATGAGTCTTGCCAAACTCCAGTTGATCAAGTTCTAAGTGAAAAAGCAAGTGGATGGGTTTATGTACTTTCCAAGATTCTCACAGAGGAAGCATCATTCAGATTTGCAAGTGAGAATGGGATTAATCTTGTATCAGTCATAACAAGTACTGTTGGGGGCAATTTTCTCACCTCCAAGGTTCCATTAAGCATTCAAGTTCTCTTATCTCCAATAACTGGTGACTCTGAACTTTATGGCATATTATCTGCTGTAAATGCAAGGATGGGATCAATTGCTTTAGTTCATGTTGAAGATATATGCAATGCCCACATCTTTTTAATGGAGCACGCCAAAGCGGAAGGTCGTTACATATGCTTTGCCCAAAGTTGTCCAATATCTAAGTTGCTTAACCATCTAGCTCAGGCGTATCCTTGCTCAAATTCAAACAGGTACAGAGTGATTGAGAAAGAAAATGATGTAGTACCTTATTCCGAGATCTCTTCAAAGAAGCTAACAGATTTGGGATTCACTTACAAGCATGGGATTGAAGATATTGTTCATCAGACAGTTTCTCGTTGTCTAGGTTATGGCTTTCTACCTCCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

372

Amino Acids

40.46

Weight (kDa)

6.51

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 33 - 172 1e-07 KR domain
Epimerase PF01370 36 - 288 4.1e-24 NAD dependent epimerase/dehydratase family
NmrA PF05368 36 - 114 6.1e-09 NmrA-like family
Polysacc_synt_2 PF02719 36 - 182 1.6e-06 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 37 - 165 2.4e-14 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 37 - 159 7.1e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 37 - 229 3.8e-10 Male sterility protein
NAD_binding_10 PF13460 39 - 206 6.1e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27250 AT4G27250
fragaria_vesca FvH4_3g21610
malus_domestica MD03G1214100.v1.1 MD11G1229100.v1.1
prunus_persica Prupe.4G200500_v2.0.a1 Prupe.4G200500_v2.0.a1
pyrus_communis pycom11g20310
rosa_chinensis RchiOBHm_Chr5g0037071
rosa_laevigata RLG00000033749
rosa_multiflora Rmu_ssc0000260.1_g000001
rosa_roxburghii Rroxscaffold_1G00043880
rosa_rugosa Rorug05G0160200
rosa_samantha Rh5AG252300 Rh5BG254100 Rh5CG286700 Rh5DG262600
rosa_wichuraiana Rw5G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 743
AciI CCGC 1 cut(s) 863
AclWI GGATC 2 cut(s) 188, 802
AcsI RAATTY 3 cut(s) 338, 439, 946
AfaI GTAC 6 cut(s) 465, 484, 594, 676, 959, 990
AfiI CCNNNNNNNGG 1 cut(s) 743
AgsI TTSAA 8 cut(s) 33, 343, 380, 722, 818, 951, 1011, 1055
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 8 cut(s) 92, 119, 214, 260, 282, 521, 926, 1018
AluI AGCT 8 cut(s) 92, 119, 214, 260, 282, 521, 926, 1018
Alw21I GWGCWC 1 cut(s) 855
AlwI GGATC 2 cut(s) 188, 802
ApeKI GCWGC 3 cut(s) 62, 211, 326
ApoI RAATTY 3 cut(s) 338, 439, 946
AseI ATTAAT 2 cut(s) 27, 651
AsuHPI GGTGA 5 cut(s) 157, 460, 463, 688, 758
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 855
BbvI GCAGC 3 cut(s) 74, 223, 338
BccI CCATC 4 cut(s) 494, 576, 784, 927
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 158
BciVI GTATCC 1 cut(s) 945
BclI TGATCA 1 cut(s) 553
BfaI CTAG 2 cut(s) 923, 1088
BfmI CTRYAG 1 cut(s) 209
BfuI GTATCC 1 cut(s) 945
BglII AGATCT 1 cut(s) 1002
BisI GCNGC 3 cut(s) 63, 212, 327
BlsI GCNGC 3 cut(s) 64, 213, 328
BmcAI AGTACT 2 cut(s) 484, 676
Bme1390I CCNGG 1 cut(s) 158
BmiI GGNNCC 2 cut(s) 412, 708
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 2 cut(s) 338, 629
BpiI GAAGAC 1 cut(s) 155
BpmI CTGGAG 2 cut(s) 179, 531
Bpu10I CCTNAGC 1 cut(s) 927
BsaJI CCNNGG 2 cut(s) 66, 702
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 1 cut(s) 743
Bse1I ACTGG 3 cut(s) 127, 548, 748
Bse3DI GCAATG 1 cut(s) 835
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 2 cut(s) 66, 702
BseGI GGATG 3 cut(s) 11, 587, 795
BseLI CCNNNNNNNGG 1 cut(s) 743
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 1 cut(s) 941
BseNI ACTGG 3 cut(s) 127, 548, 748
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 3 cut(s) 74, 223, 338
BsiHKAI GWGCWC 1 cut(s) 855
BsiSI CCGG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 743
BsmI GAATGC 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 855
Bsp143I GATC 5 cut(s) 193, 238, 553, 794, 1002
BspACI CCGC 1 cut(s) 863
BspCNI CTCAG 1 cut(s) 940
BspLI GGNNCC 2 cut(s) 412, 708
BspMAI CTGCAG 1 cut(s) 213
BspPI GGATC 2 cut(s) 188, 802
BsrDI GCAATG 1 cut(s) 835
BsrI ACTGG 3 cut(s) 127, 548, 748
BssECI CCNNGG 2 cut(s) 66, 702
BssMI GATC 5 cut(s) 193, 238, 553, 794, 1002
BssT1I CCWWGG 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 6 cut(s) 107, 187, 352, 451, 679, 1075
Bst6I CTCTTC 2 cut(s) 478, 1012
BstC8I GCNNGC 2 cut(s) 399, 855
BstDEI CTNAG 4 cut(s) 415, 563, 906, 927
BstF5I GGATG 3 cut(s) 11, 587, 795
BstKTI GATC 5 cut(s) 196, 241, 556, 797, 1005
BstMBI GATC 5 cut(s) 193, 238, 553, 794, 1002
BstMWI GCNNNNNNNGC 2 cut(s) 309, 782
BstNI CCWGG 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 156
BstSFI CTRYAG 1 cut(s) 209
BstV1I GCAGC 3 cut(s) 74, 223, 338
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 1002
BstYI RGATCY 1 cut(s) 1002
BsuI GTATCC 1 cut(s) 945
BtsCI GGATG 3 cut(s) 11, 587, 795
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 2 cut(s) 234, 489
Cac8I GCNNGC 2 cut(s) 399, 855
Csp6I GTAC 6 cut(s) 464, 483, 593, 675, 958, 989
CviAII CATG 3 cut(s) 179, 812, 1046
CviQI GTAC 6 cut(s) 464, 483, 593, 675, 958, 989
DdeI CTNAG 4 cut(s) 415, 563, 906, 927
DpnI GATC 5 cut(s) 195, 240, 555, 796, 1004
DpnII GATC 5 cut(s) 193, 238, 553, 794, 1002
Eam1104I CTCTTC 2 cut(s) 478, 1012
EarI CTCTTC 2 cut(s) 478, 1012
Eco130I CCWWGG 1 cut(s) 702
EcoRI GAATTC 2 cut(s) 338, 439
EcoRII CCWGG 1 cut(s) 156
EcoT14I CCWWGG 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 702
FaeI CATG 3 cut(s) 182, 815, 1049
FatI CATG 3 cut(s) 178, 811, 1045
FauNDI CATATG 1 cut(s) 878
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 3 cut(s) 63, 212, 327
FokI GGATG 2 cut(s) 594, 802
Fsp4HI GCNGC 3 cut(s) 63, 212, 327
FspBI CTAG 2 cut(s) 923, 1088
GluI GCNGC 3 cut(s) 63, 212, 327
GsuI CTGGAG 2 cut(s) 179, 531
HapII CCGG 1 cut(s) 114
Hin1II CATG 3 cut(s) 182, 815, 1049
HindIII AAGCTT 1 cut(s) 280
HinfI GANTC 4 cut(s) 533, 604, 749, 1032
HpaII CCGG 1 cut(s) 114
HphI GGTGA 5 cut(s) 157, 460, 463, 688, 758
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 5 cut(s) 257, 629, 754, 1000, 1071
Hpy188III TCNNGA 2 cut(s) 191, 444
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 4 cut(s) 78, 269, 292, 860
HpyCH4III ACNGT 6 cut(s) 107, 187, 352, 451, 679, 1075
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 7 cut(s) 182, 211, 326, 401, 635, 785, 828
HpyF10VI GCNNNNNNNGC 2 cut(s) 309, 782
HpyF3I CTNAG 4 cut(s) 415, 563, 906, 927
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 3 cut(s) 182, 815, 1049
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 5 cut(s) 193, 238, 553, 794, 1002
LmnI GCTCC 3 cut(s) 89, 116, 850
Lsp1109I GCAGC 3 cut(s) 74, 223, 338
LweI GCATC 2 cut(s) 338, 629
MaeI CTAG 2 cut(s) 923, 1088
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 6 cut(s) 109, 305, 346, 352, 746, 872
MalI GATC 5 cut(s) 195, 240, 555, 796, 1004
MboI GATC 5 cut(s) 193, 238, 553, 794, 1002
MboII GAAGA 5 cut(s) 160, 465, 830, 999, 1067
MfeI CAATTG 1 cut(s) 798
MflI RGATCY 1 cut(s) 1002
MhlI GDGCHC 1 cut(s) 855
MluCI AATT 6 cut(s) 28, 338, 439, 688, 798, 946
MlyI GAGTC 2 cut(s) 542, 743
MseI TTAA 5 cut(s) 27, 651, 713, 845, 915
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 1 cut(s) 158
MunI CAATTG 1 cut(s) 798
Mva1269I GAATGC 1 cut(s) 717
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 2 cut(s) 309, 782
NdeI CATATG 1 cut(s) 878
NdeII GATC 5 cut(s) 193, 238, 553, 794, 1002
NlaIII CATG 3 cut(s) 182, 815, 1049
NlaIV GGNNCC 2 cut(s) 412, 708
NmuCI GTSAC 2 cut(s) 305, 746
PctI GAATGC 1 cut(s) 717
PfeI GAWTC 2 cut(s) 604, 1032
PflMI CCANNNNNTGG 1 cut(s) 743
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 3 cut(s) 64, 213, 328
PleI GAGTC 2 cut(s) 541, 743
PpsI GAGTC 2 cut(s) 541, 743
PshBI ATTAAT 2 cut(s) 27, 651
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 412, 708
PstI CTGCAG 1 cut(s) 213
PsuI RGATCY 1 cut(s) 1002
RsaI GTAC 6 cut(s) 465, 484, 594, 676, 959, 990
RsaNI GTAC 6 cut(s) 464, 483, 593, 675, 958, 989
SaqAI TTAA 5 cut(s) 27, 651, 713, 845, 915
SatI GCNGC 3 cut(s) 63, 212, 327
Sau3AI GATC 5 cut(s) 193, 238, 553, 794, 1002
ScaI AGTACT 2 cut(s) 484, 676
SchI GAGTC 2 cut(s) 542, 743
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 1 cut(s) 855
SfaNI GCATC 2 cut(s) 338, 629
SfcI CTRYAG 1 cut(s) 209
Sse9I AATT 6 cut(s) 28, 338, 439, 688, 798, 946
SsiI CCGC 1 cut(s) 863
SspMI CTAG 2 cut(s) 923, 1088
StyD4I CCNGG 1 cut(s) 156
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 6 cut(s) 107, 187, 352, 451, 679, 1075
TaiI ACGT 1 cut(s) 324
TaqI TCGA 1 cut(s) 285
TasI AATT 6 cut(s) 28, 338, 439, 688, 798, 946
TatI WGTACW 4 cut(s) 463, 482, 592, 674
TfiI GAWTC 2 cut(s) 604, 1032
Tru1I TTAA 5 cut(s) 27, 651, 713, 845, 915
Tru9I TTAA 5 cut(s) 27, 651, 713, 845, 915
TscAI CASTG 2 cut(s) 241, 496
TseFI GTSAC 2 cut(s) 305, 746
TseI GCWGC 3 cut(s) 62, 211, 326
Tsp45I GTSAC 2 cut(s) 305, 746
TspDTI ATGAA 3 cut(s) 167, 800, 1055
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 2 cut(s) 241, 496
Van91I CCANNNNNTGG 1 cut(s) 743
VspI ATTAAT 2 cut(s) 27, 651
XapI RAATTY 3 cut(s) 338, 439, 946
XspI CTAG 2 cut(s) 923, 1088
ZrmI AGTACT 2 cut(s) 484, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.