pycom11g21820

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0014880
Physical Location & Seq
Forward (+)
24843460 .. 24845894
2435 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1695 bp
ATGATTGCTATCTTATTCATAAATATCGACTTTTTGGAGAGTTGGAAGAATTTGATTGCTAAATTCAAGGGGAGGGAGGATGGAGATGAGCTGCTGCAGGGGGTGGTTTTGGATATGGAGGTTATGGAACCATCTGATGCTCTCATGCTTTGGAACCGTAGCCTGGGTGAATATCGTGAAATGGAGATGCAAATAGAGGAAGAAGATGTGGGAGGGAAGGCGAAGAAAAAACCTCCTTTCGGTACAGTGAAGATAAATTGTGATGCGGCGTGGAATGCCAAGAGGGGGACTGGTGCTGTGGGGTGGGTGATTCGTGACTTCGCGGGATTGCTGAAGGCGGCGGGAGGTACGACTTTGATTCAAGCATCTAATGCTCAGATGGCTGAGGCGGTGGCGGTAAGGGATGCGCTAGGGGGTTGCAGGAGAGAGGGATTTACAAAGGTAATTATCGAAACGATTATTGATGCGCAACTGGAAGGTATTTTGCATGATATTTGGCACCTTGCAATTGGTATAGAATCCGTGGAGTTTGTGTTTTTTCATCGAGAAATCAACATAGTTGTTGCAACTTCCATGCAATTTGTAGGGAGAATTTTTATTTGGAATTGTATTGCGTTTGATATAAAAAAATTTGGGGAATTCATATCGGGATATGAGCGAGGGAACAAGTCTAACGGCTGGCACAGTGAGAATAACGAGAAAATTTTCATTGTGATCGAAACACGGATGGTATATTACATATATTTTACTATTTGCATAATAACAGCTAATATATCATTCTGTGTTTCAATCATACTAAAAAAGATAATTTTTTTTTTTTTCATACTAAAAAATATCTCGAGAACTAGACTATGCCGAGGAGCACCGCAATCGCGCGCACATATTTCCTTGTCAGTGTCTCTATACTCACCCAAGCTGGCTTGGTTTTGTGCAGCGGCTAGGAGTAGTAGGAGCTGCTTAACTTTTAACGGCTCTTTATCTCTATGCTGCTTTTTCTTTTTCTGCTTTTTGACCAAATATAAAACAATGAAGAGTCACATATTAATAGGCGTTGATTTATTTGCAAAAGTTGATGATTTTTTTTTTTGTTTAACATGTCAAACGGATACATATTTAGATCTGTTTGAGATGAGGTATGGCTCATCCAAACTTCTAATGTTTAATCTTCTAGACAAAGACTTGTATGACACACTTACACTAATAGCAGGTCTGTTGTATCGTGTGCCACCCACCCTAACATTAACCTCACTCAATGATAACTTTTGTTTAATAGACGCGTTAAAGTATTTTGTGAGGAGTATGATAAGCTACAATAGAATTAAGGAAGTGAAATTCACACATCTTTTTCTCTCTCTTTATACAGTGAACAGCGAAAATAATTTTTCTAGAATTTTATCTTACCAAATTGCAATCATATATGATAAATTAGAAACTTATCAATGTAGATTATCACACCACAAACTTAATTTTTTAGAACATTCATTTTTACACCGAAATTCTAAATTTAATTATCTTTCAATCGATATTCGATACCTTTGTCTTAAAGTCGGAAAAAGAGAGGAAGAAAGAGAGAGGACTTCATTAAACAGTACTCCAAATCTTCAAAAACCCTCAGGGGACCTTCACACACTCCCAGCGACACTCCCTCCTCCTGCTGTCTTTTCATCTCTGTCTCTCCCTCGCTCTCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

565

Amino Acids

64.57

Weight (kDa)

8.53

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 86 - 188 5.1e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 468
Acc36I ACCTGC 1 cut(s) 1196
AccB1I GGYRCC 1 cut(s) 498
AccII CGCG 4 cut(s) 323, 874, 876, 1277
AciI CCGC 8 cut(s) 266, 323, 338, 341, 389, 395, 866, 935
AcuI CTGAAG 1 cut(s) 353
AfaI GTAC 3 cut(s) 244, 349, 1591
AfiI CCNNNNNNNGG 3 cut(s) 163, 239, 285
AflIII ACRYGT 2 cut(s) 1094, 1275
AgsI TTSAA 5 cut(s) 67, 362, 789, 1518, 1604
AjnI CCWGG 1 cut(s) 162
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 5 cut(s) 91, 767, 916, 954, 1308
AluI AGCT 5 cut(s) 91, 767, 916, 954, 1308
Alw21I GWGCWC 1 cut(s) 865
Alw26I GTCTC 2 cut(s) 903, 1677
Ama87I CYCGRG 1 cut(s) 838
ApeKI GCWGC 5 cut(s) 91, 94, 932, 954, 987
AseI ATTAAT 1 cut(s) 1043
Asp700I GAANNNNTTC 1 cut(s) 704
AspLEI GCGC 4 cut(s) 409, 469, 876, 878
AspS9I GGNCC 1 cut(s) 1618
AsuHPI GGTGA 3 cut(s) 179, 319, 900
AvaI CYCGRG 1 cut(s) 838
AvaII GGWCC 1 cut(s) 1618
AxyI CCTNAGG 1 cut(s) 1612
BaeI ACNNNNGTAYC 2 cut(s) 1522, 1555
BanI GGYRCC 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 865
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 5 cut(s) 78, 81, 941, 944, 974
BccI CCATC 4 cut(s) 74, 139, 373, 721
BceAI ACGGC 2 cut(s) 691, 985
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 1 cut(s) 164
BciVI GTATCC 1 cut(s) 1099
BcoDI GTCTC 2 cut(s) 903, 1677
BfaI CTAG 5 cut(s) 410, 846, 939, 1169, 1386
BfmI CTRYAG 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 1196
BfuI GTATCC 1 cut(s) 1099
BglII AGATCT 1 cut(s) 1117
BisI GCNGC 8 cut(s) 92, 95, 267, 339, 933, 936, 955, 988
BlsI GCNGC 8 cut(s) 93, 96, 268, 340, 934, 937, 956, 989
BmcAI AGTACT 1 cut(s) 1591
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 1618
BmeT110I CYCGRG 1 cut(s) 838
BmgT120I GGNCC 1 cut(s) 1618
BmiI GGNNCC 4 cut(s) 129, 155, 500, 1619
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 6 cut(s) 127, 177, 253, 374, 394, 454
Bpu10I CCTNAGC 1 cut(s) 384
Bsa29I ATCGAT 1 cut(s) 1521
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 163, 522, 856
BsaXI ACNNNNNCTCC 4 cut(s) 518, 548, 1630, 1660
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 239, 285
Bse1I ACTGG 2 cut(s) 295, 477
Bse21I CCTNAGG 1 cut(s) 1612
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 1 cut(s) 164
BseCI ATCGAT 1 cut(s) 1521
BseDI CCNNGG 3 cut(s) 163, 522, 856
BseGI GGATG 4 cut(s) 85, 409, 732, 1142
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 3 cut(s) 163, 239, 285
BseMII CTCAG 3 cut(s) 375, 389, 1626
BseNI ACTGG 2 cut(s) 295, 477
BsePI GCGCGC 1 cut(s) 874
BseRI GAGGAG 3 cut(s) 873, 1309, 1638
BseXI GCAGC 5 cut(s) 78, 81, 941, 944, 974
BseYI CCCAGC 1 cut(s) 1633
BsgI GTGCAG 1 cut(s) 951
Bsh1236I CGCG 4 cut(s) 323, 874, 876, 1277
BshNI GGYRCC 1 cut(s) 498
BshVI ATCGAT 1 cut(s) 1521
BsiHKAI GWGCWC 1 cut(s) 865
BsiHKCI CYCGRG 1 cut(s) 838
BslFI GGGAC 2 cut(s) 301, 1631
BslI CCNNNNNNNGG 3 cut(s) 163, 239, 285
BsmAI GTCTC 2 cut(s) 903, 1677
BsmFI GGGAC 2 cut(s) 301, 1631
BsmI GAATGC 1 cut(s) 280
BsoBI CYCGRG 1 cut(s) 838
Bsp1286I GDGCHC 1 cut(s) 865
Bsp143I GATC 2 cut(s) 714, 1117
BspACI CCGC 8 cut(s) 266, 323, 338, 341, 389, 395, 866, 935
BspCNI CTCAG 3 cut(s) 376, 388, 1625
BspDI ATCGAT 1 cut(s) 1521
BspFNI CGCG 4 cut(s) 323, 874, 876, 1277
BspLI GGNNCC 4 cut(s) 129, 155, 500, 1619
BspMAI CTGCAG 1 cut(s) 99
BspMI ACCTGC 1 cut(s) 1196
BspT107I GGYRCC 1 cut(s) 498
BsrI ACTGG 2 cut(s) 295, 477
BssECI CCNNGG 3 cut(s) 163, 522, 856
BssHII GCGCGC 1 cut(s) 874
BssMI GATC 2 cut(s) 714, 1117
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 5 cut(s) 158, 247, 686, 1363, 1589
Bst6I CTCTTC 1 cut(s) 1025
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 3 cut(s) 680, 876, 918
BstDEI CTNAG 3 cut(s) 375, 384, 1612
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 4 cut(s) 85, 409, 732, 1142
BstFNI CGCG 4 cut(s) 323, 874, 876, 1277
BstHHI GCGC 4 cut(s) 409, 469, 876, 878
BstKTI GATC 2 cut(s) 717, 1120
BstMAI GTCTC 2 cut(s) 903, 1677
BstMBI GATC 2 cut(s) 714, 1117
BstMWI GCNNNNNNNGC 3 cut(s) 275, 371, 380
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 1098
BstSCI CCNGG 1 cut(s) 162
BstSFI CTRYAG 1 cut(s) 95
BstUI CGCG 4 cut(s) 323, 874, 876, 1277
BstV1I GCAGC 5 cut(s) 78, 81, 941, 944, 974
BstX2I RGATCY 1 cut(s) 1117
BstYI RGATCY 1 cut(s) 1117
Bsu15I ATCGAT 1 cut(s) 1521
Bsu36I CCTNAGG 1 cut(s) 1612
BsuI GTATCC 1 cut(s) 1099
BsuTUI ATCGAT 1 cut(s) 1521
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 4 cut(s) 85, 409, 732, 1142
BtsIMutI CAGTG 4 cut(s) 252, 691, 900, 1368
BveI ACCTGC 1 cut(s) 1196
Cac8I GCNNGC 3 cut(s) 680, 876, 918
CfoI GCGC 4 cut(s) 409, 469, 876, 878
Cfr13I GGNCC 1 cut(s) 1618
ClaI ATCGAT 1 cut(s) 1521
CseI GACGC 1 cut(s) 1283
Csp6I GTAC 3 cut(s) 243, 348, 1590
CviAII CATG 4 cut(s) 145, 488, 574, 1095
CviQI GTAC 3 cut(s) 243, 348, 1590
DdeI CTNAG 3 cut(s) 375, 384, 1612
DpnI GATC 2 cut(s) 716, 1119
DpnII GATC 2 cut(s) 714, 1117
Eam1104I CTCTTC 1 cut(s) 1025
EarI CTCTTC 1 cut(s) 1025
Eco47I GGWCC 1 cut(s) 1618
Eco57I CTGAAG 1 cut(s) 353
Eco81I CCTNAGG 1 cut(s) 1612
Eco88I CYCGRG 1 cut(s) 838
EcoO109I RGGNCCY 1 cut(s) 1618
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 162
FaeI CATG 4 cut(s) 148, 491, 577, 1098
FaqI GGGAC 2 cut(s) 301, 1631
FatI CATG 4 cut(s) 144, 487, 573, 1094
FauI CCCGC 2 cut(s) 316, 334
Fnu4HI GCNGC 8 cut(s) 92, 95, 267, 339, 933, 936, 955, 988
FokI GGATG 4 cut(s) 92, 416, 739, 1129
Fsp4HI GCNGC 8 cut(s) 92, 95, 267, 339, 933, 936, 955, 988
FspBI CTAG 5 cut(s) 410, 846, 939, 1169, 1386
FspI TGCGCA 1 cut(s) 468
GlaI GCGC 4 cut(s) 408, 468, 875, 877
GluI GCNGC 8 cut(s) 92, 95, 267, 339, 933, 936, 955, 988
GsaI CCCAGC 1 cut(s) 1637
HgaI GACGC 1 cut(s) 1283
HhaI GCGC 4 cut(s) 409, 469, 876, 878
Hin1II CATG 4 cut(s) 148, 491, 577, 1098
Hin6I GCGC 4 cut(s) 407, 467, 874, 876
HinP1I GCGC 4 cut(s) 407, 467, 874, 876
HinfI GANTC 4 cut(s) 310, 358, 518, 1033
HphI GGTGA 3 cut(s) 179, 319, 900
Hpy166II GTNNAC 1 cut(s) 1366
Hpy188I TCNGA 3 cut(s) 136, 378, 1550
Hpy188III TCNNGA 8 cut(s) 176, 314, 545, 648, 838, 840, 1169, 1386
Hpy8I GTNNAC 1 cut(s) 1366
HpyAV CCTTC 4 cut(s) 211, 328, 470, 1631
HpyCH4III ACNGT 5 cut(s) 158, 247, 686, 1363, 1589
HpyF10VI GCNNNNNNNGC 3 cut(s) 275, 371, 380
HpyF3I CTNAG 3 cut(s) 375, 384, 1612
Hsp92II CATG 4 cut(s) 148, 491, 577, 1098
HspAI GCGC 4 cut(s) 407, 467, 874, 876
Kzo9I GATC 2 cut(s) 714, 1117
LmnI GCTCC 2 cut(s) 860, 951
Lsp1109I GCAGC 5 cut(s) 78, 81, 941, 944, 974
LweI GCATC 6 cut(s) 127, 177, 253, 374, 394, 454
MaeI CTAG 5 cut(s) 410, 846, 939, 1169, 1386
MaeIII GTNAC 2 cut(s) 314, 1034
MalI GATC 2 cut(s) 716, 1119
MboI GATC 2 cut(s) 714, 1117
MboII GAAGA 9 cut(s) 58, 212, 215, 235, 262, 1042, 1157, 1574, 1592
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 1117
MhlI GDGCHC 1 cut(s) 865
MluI ACGCGT 1 cut(s) 1275
MlyI GAGTC 1 cut(s) 1042
MmeI TCCRAC 2 cut(s) 23, 1528
MroXI GAANNNNTTC 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 935
MspR9I CCNGG 1 cut(s) 164
MunI CAATTG 1 cut(s) 507
Mva1269I GAATGC 1 cut(s) 280
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 4 cut(s) 323, 874, 876, 1277
MwoI GCNNNNNNNGC 3 cut(s) 275, 371, 380
NdeII GATC 2 cut(s) 714, 1117
NlaIII CATG 4 cut(s) 148, 491, 577, 1098
NlaIV GGNNCC 4 cut(s) 129, 155, 500, 1619
NmeAIII GCCGAG 1 cut(s) 881
NmuCI GTSAC 2 cut(s) 314, 1034
NsbI TGCGCA 1 cut(s) 468
NspI RCATGY 1 cut(s) 1098
PaeR7I CTCGAG 1 cut(s) 838
PauI GCGCGC 1 cut(s) 874
PciI ACATGT 1 cut(s) 1094
PctI GAATGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 704
PfeI GAWTC 3 cut(s) 310, 358, 518
PkrI GCNGC 8 cut(s) 93, 96, 268, 340, 934, 937, 956, 989
PleI GAGTC 1 cut(s) 1041
PpsI GAGTC 1 cut(s) 1041
PpuMI RGGWCCY 1 cut(s) 1618
PscI ACATGT 1 cut(s) 1094
PshBI ATTAAT 1 cut(s) 1043
Psp5II RGGWCCY 1 cut(s) 1618
Psp6I CCWGG 1 cut(s) 162
PspFI CCCAGC 1 cut(s) 1633
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 4 cut(s) 129, 155, 500, 1619
PspPI GGNCC 1 cut(s) 1618
PspPPI RGGWCCY 1 cut(s) 1618
PstI CTGCAG 1 cut(s) 99
PsuI RGATCY 1 cut(s) 1117
PteI GCGCGC 1 cut(s) 874
RsaI GTAC 3 cut(s) 244, 349, 1591
RsaNI GTAC 3 cut(s) 243, 348, 1590
SatI GCNGC 8 cut(s) 92, 95, 267, 339, 933, 936, 955, 988
Sau3AI GATC 2 cut(s) 714, 1117
Sau96I GGNCC 1 cut(s) 1618
ScaI AGTACT 1 cut(s) 1591
SchI GAGTC 1 cut(s) 1042
ScrFI CCNGG 1 cut(s) 164
SduI GDGCHC 1 cut(s) 865
SfaNI GCATC 6 cut(s) 127, 177, 253, 374, 394, 454
SfcI CTRYAG 1 cut(s) 95
Sfr274I CTCGAG 1 cut(s) 838
SinI GGWCC 1 cut(s) 1618
SlaI CTCGAG 1 cut(s) 838
SmlI CTYRAG 1 cut(s) 838
SmoI CTYRAG 1 cut(s) 838
SsiI CCGC 8 cut(s) 266, 323, 338, 341, 389, 395, 866, 935
SspMI CTAG 5 cut(s) 410, 846, 939, 1169, 1386
StyD4I CCNGG 1 cut(s) 162
TaaI ACNGT 5 cut(s) 158, 247, 686, 1363, 1589
TaqI TCGA 7 cut(s) 27, 450, 544, 717, 839, 1521, 1528
TatI WGTACW 1 cut(s) 1589
TauI GCSGC 3 cut(s) 269, 341, 938
TfiI GAWTC 3 cut(s) 310, 358, 518
TscAI CASTG 4 cut(s) 252, 691, 900, 1368
TseFI GTSAC 2 cut(s) 314, 1034
TseI GCWGC 5 cut(s) 91, 94, 932, 954, 987
Tsp45I GTSAC 2 cut(s) 314, 1034
TspDTI ATGAA 9 cut(s) 7, 530, 631, 697, 811, 1043, 1470, 1569, 1653
TspGWI ACGGA 3 cut(s) 511, 739, 1118
TspRI CASTG 4 cut(s) 252, 691, 900, 1368
VpaK11BI GGWCC 1 cut(s) 1618
VspI ATTAAT 1 cut(s) 1043
XbaI TCTAGA 2 cut(s) 1168, 1385
XceI RCATGY 1 cut(s) 1098
XhoI CTCGAG 1 cut(s) 838
XmnI GAANNNNTTC 1 cut(s) 704
XspI CTAG 5 cut(s) 410, 846, 939, 1169, 1386
ZrmI AGTACT 1 cut(s) 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.