pycom11g24850

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010707
Physical Location & Seq
Forward (+)
27703635 .. 27706167
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1110 bp
ATGGGCAGCAGCAGCGGCGCTAAAACGCCGTCGTTAATGAGGGACTTTCTCAGGCATGCGGGCGGCATCGCGGTGGTCGACGGTGGACTTGCCACCGAGCTCGAACGCCACGGTGCTGACCTCAACGATCCTCTCTGGAGCGCCAAATGCCTTCTCACTTCCCCTCACCTCATTCGCACGGTACACATTGATTACCTTGAAGCTGGTGCAGATATCATAATCACAGCGTCCTATCAGGTAATGCCGGTTACTAATAATGGCTCTCTTAATTTATATTTTTTTGTGAAGCTGAAAGTGTTAGTTGCTATGCAGGCCACCATTCAAGGTTTCGAGGCCAAAGGATTTTCAACAGAAGAGAGCGAAGCCTTGCTTCGGAAAAGTGTTGAAATTGCCCGTGAGGCACGTGATGTGTATTACGACAGATGTACGCAATGCTCTTCTGCTGACAGTGCAAGCGGTAGAATTCTCAAGCGTAGGCCAATCTTAGTTGCAGCGTCTGTGGGGAGCTATGGTGCTTATTTGGCTGATGGGTCTGAGTACAGTGGAGATTATGGTGAGGCTATGACTCTAGGAGGTTTAAAAGATTTTCATCGGAGAAGGGTCCAGGTCCTAGCAGAATCAGGTCCTGACTTACTTGCATTTGAAACGGTTCCAAATAAGCTGGAGGCTCAGGCTTATGCTGAGCTTTTGGAAGAAGAAAACATGCAACTTCCTGCGTGGTTTTCTTTCAACTCTAAAGATGGCATCAATGTTGTTAGTGGTGATTCTCTCCTTGAATGCGCCTCAGTTGCTGAATCATGCAATAAAGTCGTTGCAGTTGGAATCAACTGCACCCCCCCTAGATTTATCCACGGACTGTTAACACTAATTACACAGGTGGCTACAAAACCAATTATTGTGTACCCAAATAGTGGTGAAAGCTATGATGCAGATAGAATGATGTGGGTGCAAAACACTGGGGTTTCAGATGAAGATTTTGTCTCGTATGTAAACAAATGGTGTGAGGTAGGGGCTTCCCTTGTAGGAGGTTGTTGCAGAACAACCCCAAATACAATCAGAGCAATATACAGGACTCTTCACAATAGGTCAACATCTCCACCAGAGCAGTAA

Protein Analysis

370

Amino Acids

40.14

Weight (kDa)

5.28

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 25 - 358 1.5e-75 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 911
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 71
AciI CCGC 5 cut(s) 15, 59, 63, 71, 456
AclWI GGATC 1 cut(s) 122
AcsI RAATTY 1 cut(s) 462
AcvI CACGTG 1 cut(s) 404
AfaI GTAC 4 cut(s) 183, 427, 539, 902
AfiI CCNNNNNNNGG 2 cut(s) 372, 911
AgsI TTSAA 7 cut(s) 200, 323, 348, 386, 644, 730, 776
AjnI CCWGG 1 cut(s) 603
AluBI AGCT 7 cut(s) 100, 203, 289, 507, 661, 685, 921
AluI AGCT 7 cut(s) 100, 203, 289, 507, 661, 685, 921
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 985
AlwI GGATC 1 cut(s) 122
AlwNI CAGNNNCTG 3 cut(s) 497, 626, 791
AoxI GGCC 3 cut(s) 312, 333, 476
ApeKI GCWGC 4 cut(s) 6, 9, 12, 491
ApoI RAATTY 1 cut(s) 462
Asp700I GAANNNNTTC 1 cut(s) 648
AspLEI GCGC 3 cut(s) 20, 143, 782
AspS9I GGNCC 3 cut(s) 601, 607, 623
AsuHPI GGTGA 4 cut(s) 158, 566, 773, 926
AvaII GGWCC 3 cut(s) 601, 607, 623
BanII GRGCYC 1 cut(s) 102
BbrPI CACGTG 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 4 cut(s) 18, 21, 24, 503
BccI CCATC 2 cut(s) 521, 734
BceAI ACGGC 1 cut(s) 13
BcgI CGANNNNNNTGC 2 cut(s) 790, 824
BciT130I CCWGG 1 cut(s) 605
BcoDI GTCTC 1 cut(s) 985
BfaI CTAG 3 cut(s) 569, 611, 840
BfoI RGCGCY 2 cut(s) 21, 144
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 6 cut(s) 7, 10, 13, 16, 64, 492
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 6 cut(s) 8, 11, 14, 17, 65, 493
Bme1390I CCNGG 1 cut(s) 605
Bme18I GGWCC 3 cut(s) 601, 607, 623
BmgT120I GGNCC 3 cut(s) 601, 607, 623
BmiI GGNNCC 2 cut(s) 602, 651
BmrFI CCNGG 1 cut(s) 605
BmrI ACTGGG 1 cut(s) 966
BmsI GCATC 3 cut(s) 75, 753, 916
BmuI ACTGGG 1 cut(s) 966
BpmI CTGGAG 2 cut(s) 157, 683
Bpu10I CCTNAGC 1 cut(s) 669
Bpu1102I GCTNAGC 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 452
BsaAI YACGTR 1 cut(s) 404
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 2 cut(s) 109, 850
BsaXI ACNNNNNCTCC 2 cut(s) 496, 526
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 911
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 1 cut(s) 961
Bse3DI GCAATG 1 cut(s) 437
Bse8I GATNNNNATC 1 cut(s) 588
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 109, 850
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 2 cut(s) 372, 911
BseMI GCAATG 1 cut(s) 437
BseMII CTCAG 5 cut(s) 64, 525, 672, 683, 798
BseNI ACTGG 1 cut(s) 961
BseXI GCAGC 4 cut(s) 18, 21, 24, 503
BsgI GTGCAG 2 cut(s) 228, 814
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 314, 335, 478
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 372, 911
BsmAI GTCTC 1 cut(s) 985
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 3 cut(s) 314, 335, 478
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 1 cut(s) 127
Bsp1720I GCTNAGC 1 cut(s) 681
BspACI CCGC 5 cut(s) 15, 59, 63, 71, 456
BspANI GGCC 3 cut(s) 314, 335, 478
BspCNI CTCAG 5 cut(s) 63, 526, 673, 682, 797
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 602, 651
BspPI GGATC 1 cut(s) 122
BspQI GCTCTTC 1 cut(s) 442
BsrDI GCAATG 1 cut(s) 437
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 1 cut(s) 961
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 109, 850
BssMI GATC 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 7 cut(s) 83, 113, 181, 449, 542, 649, 858
Bst6I CTCTTC 3 cut(s) 348, 442, 1080
BstBAI YACGTR 1 cut(s) 404
BstC8I GCNNGC 4 cut(s) 57, 61, 312, 454
BstDEI CTNAG 6 cut(s) 50, 484, 534, 669, 681, 784
BstDSI CCRYGG 2 cut(s) 109, 850
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 2 cut(s) 21, 144
BstHHI GCGC 3 cut(s) 20, 143, 782
BstKTI GATC 1 cut(s) 130
BstMAI GTCTC 1 cut(s) 985
BstMBI GATC 1 cut(s) 127
BstMWI GCNNNNNNNGC 8 cut(s) 12, 15, 147, 311, 398, 449, 521, 788
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 2 cut(s) 59, 706
BstSCI CCNGG 1 cut(s) 603
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 4 cut(s) 18, 21, 24, 503
BsuRI GGCC 3 cut(s) 314, 335, 478
BtgI CCRYGG 2 cut(s) 109, 850
BtgZI GCGATG 1 cut(s) 52
BtsIMutI CAGTG 3 cut(s) 454, 547, 954
Cac8I GCNNGC 4 cut(s) 57, 61, 312, 454
CaiI CAGNNNCTG 3 cut(s) 497, 626, 791
CfoI GCGC 3 cut(s) 20, 143, 782
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 3 cut(s) 601, 607, 623
CseI GACGC 2 cut(s) 216, 483
Csp6I GTAC 4 cut(s) 182, 426, 538, 901
CviAII CATG 3 cut(s) 56, 703, 798
CviQI GTAC 4 cut(s) 182, 426, 538, 901
DdeI CTNAG 6 cut(s) 50, 484, 534, 669, 681, 784
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
DraI TTTAAA 1 cut(s) 579
Eam1104I CTCTTC 3 cut(s) 348, 442, 1080
EarI CTCTTC 3 cut(s) 348, 442, 1080
Ecl136II GAGCTC 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 102
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 3 cut(s) 601, 607, 623
Eco53kI GAGCTC 1 cut(s) 100
Eco72I CACGTG 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 2 cut(s) 607, 623
EcoRI GAATTC 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 603
EcoRV GATATC 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 102
FaeI CATG 3 cut(s) 59, 706, 801
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FaqI GGGAC 1 cut(s) 56
FatI CATG 3 cut(s) 55, 702, 797
FauI CCCGC 1 cut(s) 52
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 6 cut(s) 7, 10, 13, 16, 64, 492
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 6 cut(s) 7, 10, 13, 16, 64, 492
FspBI CTAG 3 cut(s) 569, 611, 840
GlaI GCGC 3 cut(s) 19, 142, 781
GluI GCNGC 6 cut(s) 7, 10, 13, 16, 64, 492
GsuI CTGGAG 2 cut(s) 157, 683
HaeII RGCGCY 2 cut(s) 21, 144
HaeIII GGCC 3 cut(s) 314, 335, 478
HapII CCGG 1 cut(s) 245
HgaI GACGC 2 cut(s) 216, 483
HhaI GCGC 3 cut(s) 20, 143, 782
Hin1II CATG 3 cut(s) 59, 706, 801
Hin6I GCGC 3 cut(s) 18, 141, 780
HinP1I GCGC 3 cut(s) 18, 141, 780
HincII GTYRAC 3 cut(s) 79, 861, 1089
HindII GTYRAC 3 cut(s) 79, 861, 1089
HinfI GANTC 6 cut(s) 565, 617, 764, 794, 822, 1072
HpaI GTTAAC 1 cut(s) 861
HpaII CCGG 1 cut(s) 245
HphI GGTGA 4 cut(s) 158, 566, 773, 926
Hpy166II GTNNAC 7 cut(s) 79, 86, 184, 861, 901, 991, 1089
Hpy188I TCNGA 5 cut(s) 375, 535, 594, 967, 1058
Hpy188III TCNNGA 2 cut(s) 136, 626
Hpy8I GTNNAC 7 cut(s) 79, 86, 184, 861, 901, 991, 1089
Hpy99I CGWCG 2 cut(s) 34, 83
HpyAV CCTTC 2 cut(s) 161, 591
HpyCH4III ACNGT 7 cut(s) 83, 113, 181, 449, 542, 649, 858
HpyCH4IV ACGT 1 cut(s) 403
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 15, 147, 311, 398, 449, 521, 788
HpyF3I CTNAG 6 cut(s) 50, 484, 534, 669, 681, 784
HpySE526I ACGT 1 cut(s) 403
Hsp92II CATG 3 cut(s) 59, 706, 801
HspAI GCGC 3 cut(s) 18, 141, 780
KspAI GTTAAC 1 cut(s) 861
Kzo9I GATC 1 cut(s) 127
LguI GCTCTTC 1 cut(s) 442
LmnI GCTCC 2 cut(s) 138, 504
Lsp1109I GCAGC 4 cut(s) 18, 21, 24, 503
LweI GCATC 3 cut(s) 75, 753, 916
MaeI CTAG 3 cut(s) 569, 611, 840
MaeII ACGT 1 cut(s) 403
MaeIII GTNAC 1 cut(s) 247
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 6 cut(s) 365, 429, 704, 707, 983, 1067
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 5 cut(s) 268, 387, 462, 867, 891
MlyI GAGTC 2 cut(s) 559, 1066
MmeI TCCRAC 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 4 cut(s) 35, 267, 578, 860
MslI CAYNNNNRTG 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 605
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 8 cut(s) 12, 15, 147, 311, 398, 449, 521, 788
NdeII GATC 1 cut(s) 127
NlaIII CATG 3 cut(s) 59, 706, 801
NlaIV GGNNCC 2 cut(s) 602, 651
NspI RCATGY 2 cut(s) 59, 706
PaeI GCATGC 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 442
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 782
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 4 cut(s) 617, 764, 794, 822
PflMI CCANNNNNTGG 1 cut(s) 911
PkrI GCNGC 6 cut(s) 8, 11, 14, 17, 65, 493
PleI GAGTC 2 cut(s) 559, 1066
PmaCI CACGTG 1 cut(s) 404
PmlI CACGTG 1 cut(s) 404
PpsI GAGTC 2 cut(s) 559, 1066
Ppu21I YACGTR 1 cut(s) 404
PpuMI RGGWCCY 2 cut(s) 607, 623
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 2 cut(s) 607, 623
Psp6I CCWGG 1 cut(s) 603
PspCI CACGTG 1 cut(s) 404
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 2 cut(s) 602, 651
PspPI GGNCC 3 cut(s) 601, 607, 623
PspPPI RGGWCCY 2 cut(s) 607, 623
PstNI CAGNNNCTG 3 cut(s) 497, 626, 791
RsaI GTAC 4 cut(s) 183, 427, 539, 902
RsaNI GTAC 4 cut(s) 182, 426, 538, 901
RseI CAYNNNNRTG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 102
SalI GTCGAC 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 442
SaqAI TTAA 4 cut(s) 35, 267, 578, 860
SatI GCNGC 6 cut(s) 7, 10, 13, 16, 64, 492
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 3 cut(s) 601, 607, 623
SchI GAGTC 2 cut(s) 559, 1066
ScrFI CCNGG 1 cut(s) 605
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 3 cut(s) 75, 753, 916
SinI GGWCC 3 cut(s) 601, 607, 623
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SphI GCATGC 1 cut(s) 59
Sse9I AATT 5 cut(s) 268, 387, 462, 867, 891
SsiI CCGC 5 cut(s) 15, 59, 63, 71, 456
SspMI CTAG 3 cut(s) 569, 611, 840
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 603
TaaI ACNGT 7 cut(s) 83, 113, 181, 449, 542, 649, 858
TaiI ACGT 1 cut(s) 406
TaqI TCGA 3 cut(s) 78, 102, 330
TasI AATT 5 cut(s) 268, 387, 462, 867, 891
TatI WGTACW 1 cut(s) 537
TauI GCSGC 2 cut(s) 18, 66
TfiI GAWTC 4 cut(s) 617, 764, 794, 822
Tru1I TTAA 4 cut(s) 35, 267, 578, 860
Tru9I TTAA 4 cut(s) 35, 267, 578, 860
TscAI CASTG 3 cut(s) 454, 547, 961
TseI GCWGC 4 cut(s) 6, 9, 12, 491
TspDTI ATGAA 2 cut(s) 578, 984
TspGWI ACGGA 1 cut(s) 867
TspRI CASTG 3 cut(s) 454, 547, 961
Van91I CCANNNNNTGG 1 cut(s) 911
VpaK11BI GGWCC 3 cut(s) 601, 607, 623
XapI RAATTY 1 cut(s) 462
XceI RCATGY 2 cut(s) 59, 706
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 3 cut(s) 569, 611, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.