Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0006487

Physical Location & Seq

Genomic Coordinates Forward (+)

29170297 .. 29180695

Gene Structure 10399 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3768 bp

ATGGTTGAAACCAGGCGCAGCTCCTCTTCCAAACGCGCCTTATCCGCCTCCCCCCCTCCCAACCCCAAGCGCTCAAAGGCGTCTGATGCTTCATCGTCGAACAACGGAGTTAGGAGCGGGCCGCCAGCGGAGCCTTTGGGTCCGATTAAGGAATCTGGGTCTCAACCTCCGGAACTTGAGCTCCGATCCTCTGATCCGCCGACTATCGATTCGTTGAAGGCAATTAACGGCCCTGATGCCACTGCTCTGGAAAGATCCCCCGATGATGTTGCGGAAGGCGAGGCCTTGGTTTCACCGCAGCCTTTGGGGGAAACTGCAGTCCGTGCCGGTTTGAAGCGGGCCAAGAAGCTTCCGAAGAAGAATGCAAAATTGAATTCGAAATCTGCTTGGGGAATGCTTATTTCGCAGTTCTCAAAGAATCCGCACCAGTTCATTTGTGAGACTGTTTTCACTGTCGGTCAAAGTTGTGAGTGCCATTTATGTCTTAAGGACCCATCCATTAGTACTACTTTGTGTAAACTGAAACATGTTAAGCGTGACGGTTCGTCCGCTGCAGAACTAGAGATTATATGCGGTAAGGGTGATGTTCAGGTGAATGAGAAGACTTATCAAAAGGACACGAAGGTGATTCTTAATGGAGGCGATGAGGTTGTATTCGGCTCGTCTGGAAAACATGCATATATTTTCCAGCAGCTGACCAATGACCATAGTATAGCTACTCAGGGCATACCTTCAATTAGCATTTTAGAAACCCAGAATGCTCCAGTTAATGGGATGCATATGGAGGCAAGATCAGGGGACCCCTCTGCTGTTGATGGGGCTTCAATATTAGCGTCGCTGTCAAATGTGCCAAATGACTTGTCACTACTTTCAGAACCTGCTAAAGCTGGCGACGATCTGCAACAAGATGCAGAGATGCCTTCTCTTCCTTCTGCCTGTCGAGATTCAGATGATCGTACTCCAGACATTGAGATGAAGGAGACTACTAATATTAATGATCAAGTTTCAGGTGATAAAAATATTGTTCAGTACCCTGACACTGCGAATGAAAACCCCAATCTTGATAGTGTTGCATTGGATATGGATACTGAAACCCAGAAGGCCTCTGGGGCAACTTATCAACTAAGACCACTCTTCCGAATGTTTACCGGTTCTTCTAGTGCCAATTTTGACTTAACTGGCAGCATTTCTAAGATACTTGATGAGCAAAGGGAAATAAGAGAACTTCTTCACAATTTTGGTCCTCCAATGTTGATATCAACTAGGCGACAAGCATTTAAAGAAAAACTGCAACAAGGAATTCTACGTCCTGATGATATTGATGTCTCATTCGAAAGTTTTCCATATTACCTAAGTGAAACAACAAAGAAGGTTTTGATTGCATCCACCCACCCGAATTTGAGGTGTAGCAAGTTTGGAAAATATTTTTCATCACTACCTACCGGGAGCCCGCGCATATTATTATCTGGTCCAGCAGGTTCTGAGATATATCAGGAAACCTTGGCAAAGGCACTTGCGAAACATTTTGGTGCTAGATTACTAATTGTAGATTCTCTTCTCCTGCCTGGTGTTGAGGCACCACCGCCCAAGGATACTGATTCTGTTAAAGAAGTACCAAGGCTTGAAAGAGTTTCCACATTTGCGAAACGAGCAGCTCATGCTGCTGGATTCAAGCACAAGAAACCGACCTCTAGTGTTGAGGCTGAAATTACTGGCGGATCCACTGTGAGTTCTCAGGCACTGCCAAAGCAGGAGACTTCTACTGCATCATCCAGAGTCAATACTTTTAAACAAGGTGACAGGGTCAAATTTGTGGGTGCCATATCTTCTGGTCCTCAACCGCTGCAGAGTTGTCCTCTTCTAAGGGGACCATCATATGGTTGTCGAGGCAAAGTTGTTCTTGCTTTTGAAGAAAATGGGGCCTCAAAAATTGGTGTTAGATTTGATAAATCAATACCAGATGGCAATGATCTTGGTGGTCTTTGTGAAGAAGATCACGGCTTCTTTTGTTCTGCTAGTCATTTACTTCCCTTGGATGTTTCTGGAGGTGATGATATTGACAAGCTTGCAATCGGTGAACTTCTTGAGGTGGCAACCAATGAGAGTAAAAGTCTTCCTTTGATAATGTTTTTGAAAGATATAGAAAAGGTTATGGCAGGTAACCCAGATGCATATAGTGTCTTGAAGAGTAAGCTTTCAAACTTGCCAGAAGATGTTGTTGTGATCGGCTCTCATACCCAGTTGGACAATCGCAAGGAGAAGTCCCATCCTGGTAGCCTTTTGTTTACAAAGTTCGGCTTAAACCAAACAGCTTTGCTTGATCTTGCTTTTCCGGATAACCTTGGTGGTAGACTGCATGATAGGAGCAAAGAAACTCCTAAAACTATGAAGCAACTTCAACTCTCTCGGATTTTCCCCAACAAAGTGACCATACAGCTGCCCCAGGATGAGGCTTTACTTTCGGACTGGAAGCAGCAGTTGGAACGTGATGTTGAAACTTTGAAATCGCAGTCTAATATTGTTACCATTCGCGCAGTTCTTAACCGAGTTTCTGTGGATTGCCCTGACCTTGAAAGTCTGTGCATCAAAGATCTAGCGCTTACAACTGAAAGTGTTGATAAAGTAGTAGGCTGGGCTGTTAGTCACCATCTTATGCATTGTTCAGGCGCTTTAGTTAAAGATGACAAACTTGTTATTTCTACTGAAAGTCTTAGGGATGGACTAAACATTCTACAAGGCATTCAAAACGAAAACAAGAGCATAAAGAAATCACTTAAGGATGTGGTTACTGGAAATGAATTTGAGAAAAAACTTCTTGCTGATGTTATTCCACCGAGCGATATTGGGGTTACTTTTGATGACATCGGGGCCTTGGAAAATGTGAAGGACACCCTAAAGGAGTTGGTGATGCTTCCTCTTCAGAGGCCTGAATTATTTAGCAAGGGACAGTTGACTAAGCCTTGTAAAGGAATCTTGCTATTTGGTCCTCCGGGTACTGGAAAGACTATGCTTGCAAAGGCTGTTGCAACTGAAGCTGGTGCAAACTTTATTAACATATCAATGTCAAGTATTACTTCAAAGTGGTTTGGGGAAGGAGAGAAGTACGTTAAAGCGGTGTTCTCCTTAGCTAGTAAAATTGCCCCTAGTGTTGTTTTTGTTGATGAGGTTGACAGCATGTTAGGCAGACGTGAAAATCCTGGGGAACATGAGGCTATGCGTAAAATGAAAAATGAGTTCATGGTTAACTGGGATGGTCTGCGTACAAAGGATAAAGAACGTGTATTGGTACTTGCTGCTACTAATAGGCCTTTTGACCTTGATGAGGCTGTTATTAGGAGGCTTCCGAGGAGATTGATGGTGAACTTGCCAGATGCCCAGAACAGAGAAAAAATACTGAGAGTTGTATTGGCCAAAGAAGATTTGGAACTTGACGTTGATTTGGAAGCAGTTGCAAATATGACAGATGGGTATTCGGGAAGTGACTTAAAGAATCTCTGTGTTGCTGCAGCTCACCTCCCCATTAGGGAAATTTTAGAGAAAGAGAAAAAGGAGAGAATTTTTGCTTTGGCGGAGAACAGACCTGTACCGGAGTTATACTGCAGTACCGACATTCGTCCCTTGAAGATGGAGGACTTCAAACATGCGCATGAGCAGGTGTGTGCAAGTGTGTCATCGGAGTCGACAAATATGAGTGAGCTCCTCCAATGGAATGACCTATATGGCGAAGGCGGATCAAGAAAGAAGACGTCTCTCAGCTATTTTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002090 GO:0002092 GO:0003008 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0007610 GO:0007611 GO:0007612 GO:0007613 GO:0008150 GO:0009893 GO:0010604 GO:0010646 GO:0010648 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0023051 GO:0023057 GO:0030100 GO:0031323 GO:0031325 GO:0032501 GO:0032879 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044456 GO:0044459 GO:0044464 GO:0045202 GO:0045211 GO:0045807 GO:0048259 GO:0048260 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050804 GO:0050805 GO:0050877 GO:0050890 GO:0051049 GO:0051050 GO:0051128 GO:0051130 GO:0051966 GO:0051967 GO:0060255 GO:0060627 GO:0065007 GO:0071944 GO:0097060 GO:0098590 GO:0098794 GO:0099177

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA FHA

Protein Analysis

1256

Amino Acids

136.92

Weight (kDa)

6.02

Isoelectric Point (pI)

40.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7751	PF24933	852 - 912	2.2e-25	Domain of unknown function (DUF7751)
AAA	PF00004	991 - 1121	8.9e-38	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	1144 - 1180	1.8e-13	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006487)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02890 AT1G02890 AT4G02480
fragaria_vesca	FvH4_2g25490 FvH4_2g25490 FvH4_2g25490
malus_domestica	MD03G1285800.v1.1 MD11G1305200.v1.1 MD11G1305300.v1.1 MD11G1305400.v1.1
prunus_persica	Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1 Prupe.8G259000_v2.0.a1
pyrus_communis	pycom03g22650 pycom11g26700 pycom11g26720
rosa_chinensis	RchiOBHm_Chr6g0293701
rosa_laevigata	RLG00000011931
rosa_roxburghii	Rroxscaffold_7G00173960
rosa_rugosa	Rorug06G0237500
rosa_samantha	Rh6AG350200 Rh6BG357700 Rh6CG364500 Rh6DG351400
rosa_wichuraiana	Rw6G030490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3643
AatII	GACGTC	1 cut(s)	3749
Acc16I	TGCGCA	1 cut(s)	3645
Acc36I	ACCTGC	4 cut(s)	886, 1466, 2147, 3643
AccB1I	GGYRCC	2 cut(s)	1576, 1817
AccB7I	CCANNNNNTGG	2 cut(s)	770, 1877
AccBSI	CCGCTC	1 cut(s)	117
AccI	GTMKAC	2 cut(s)	2350, 3680
AccII	CGCG	3 cut(s)	36, 1453, 2532
AccIII	TCCGGA	2 cut(s)	169, 2332
AclWI	GGATC	6 cut(s)	180, 188, 249, 1713, 1726, 3739
AcoI	YGGCCR	1 cut(s)	3408
AcsI	RAATTY	7 cut(s)	373, 1299, 1396, 1808, 2798, 3528, 3555
AcuI	CTGAAG	2 cut(s)	2903, 3051
AcyI	GRCGYC	2 cut(s)	80, 3746
AfeI	AGCGCT	2 cut(s)	71, 2598
AfiI	CCNNNNNNNGG	7 cut(s)	770, 1877, 2449, 2966, 2996, 3322, 3523
AflII	CTTAAG	2 cut(s)	485, 2774
AflIII	ACRYGT	2 cut(s)	526, 3277
AgeI	ACCGGT	1 cut(s)	1148
AhdI	GACNNNNNGTC	1 cut(s)	544
AjiI	CACGTC	1 cut(s)	3188
AjnI	CCWGG	5 cut(s)	11, 1564, 2269, 2442, 3196
AjuI	GAANNNNNNNTTGG	2 cut(s)	2462, 2494
AleI	CACNNNNGTG	1 cut(s)	623
AloI	GAACNNNNNNTCC	2 cut(s)	165, 197
Alw21I	GWGCWC	2 cut(s)	183, 3699
Alw26I	GTCTC	6 cut(s)	165, 434, 974, 1330, 1748, 3753
AlwI	GGATC	6 cut(s)	180, 188, 249, 1713, 1726, 3739
AlwNI	CAGNNNCTG	4 cut(s)	694, 878, 1481, 1741
Aor13HI	TCCGGA	2 cut(s)	169, 2332
Aor51HI	AGCGCT	2 cut(s)	71, 2598
ApoI	RAATTY	7 cut(s)	373, 1299, 1396, 1808, 2798, 3528, 3555
ArsI	GACNNNNNNTTYG	4 cut(s)	193, 225, 845, 877
AseI	ATTAAT	1 cut(s)	993
AsiGI	ACCGGT	1 cut(s)	1148
Asp700I	GAANNNNTTC	2 cut(s)	1227, 1338
AspLEI	GCGC	8 cut(s)	18, 38, 72, 1455, 2534, 2599, 2669, 3646
AsuC2I	CCSGG	2 cut(s)	1444, 2991
AsuII	TTCGAA	2 cut(s)	377, 1332
AvaII	GGWCC	8 cut(s)	140, 490, 799, 1241, 1469, 1832, 1868, 2984
BaeI	ACNNNNGTAYC	2 cut(s)	3586, 3619
BalI	TGGCCA	1 cut(s)	3410
BamHI	GGATCC	1 cut(s)	1718
BanI	GGYRCC	2 cut(s)	1576, 1817
BanII	GRGCYC	3 cut(s)	183, 1451, 3699
BarI	GAAGNNNNNNTAC	2 cut(s)	1539, 1571
BbsI	GAAGAC	3 cut(s)	608, 2105, 3749
Bbv12I	GWGCWC	2 cut(s)	183, 3699
BceAI	ACGGC	2 cut(s)	244, 2014
BcgI	CGANNNNNNTGC	2 cut(s)	251, 285
BciT130I	CCWGG	5 cut(s)	13, 1566, 2271, 2444, 3198
BciVI	GTATCC	2 cut(s)	1078, 1585
BclI	TGATCA	1 cut(s)	997
BcnI	CCSGG	2 cut(s)	1444, 2991
BcoDI	GTCTC	6 cut(s)	165, 434, 974, 1330, 1748, 3753
BfaI	CTAG	9 cut(s)	560, 1158, 1263, 1533, 1692, 2016, 2594, 3129, 3144
BfmI	CTRYAG	5 cut(s)	315, 552, 1844, 3504, 3598
BfoI	RGCGCY	3 cut(s)	73, 2600, 2670
BfrI	CTTAAG	2 cut(s)	485, 2774
BfuAI	ACCTGC	4 cut(s)	886, 1466, 2147, 3643
BfuI	GTATCC	2 cut(s)	1078, 1585
BglI	GCCNNNNNGGC	1 cut(s)	1109
BglII	AGATCT	1 cut(s)	2590
BmcAI	AGTACT	1 cut(s)	505
Bme1390I	CCNGG	7 cut(s)	13, 1444, 1566, 2271, 2444, 2991, 3198
Bme18I	GGWCC	8 cut(s)	140, 490, 799, 1241, 1469, 1832, 1868, 2984
BmeRI	GACNNNNNGTC	1 cut(s)	544
BmgBI	CACGTC	1 cut(s)	3188
BmrFI	CCNGG	7 cut(s)	13, 1444, 1566, 2271, 2444, 2991, 3198
BmrI	ACTGGG	2 cut(s)	2233, 3256
BmuI	ACTGGG	2 cut(s)	2233, 3256
BoxI	GACNNNNGTC	1 cut(s)	3612
BpiI	GAAGAC	3 cut(s)	608, 2105, 3749
BplI	GAGNNNNNCTC	2 cut(s)	1840, 1872
BpmI	CTGGAG	3 cut(s)	747, 945, 2064
Bpu10I	CCTNAGC	1 cut(s)	3124
Bpu14I	TTCGAA	2 cut(s)	377, 1332
BpuEI	CTTGAG	2 cut(s)	197, 2105
BpuMI	CCSGG	2 cut(s)	1444, 2991
Bsa29I	ATCGAT	1 cut(s)	207
BsaHI	GRCGYC	2 cut(s)	80, 3746
BsaI	GGTCTC	1 cut(s)	165
BsaWI	WCCGGW	4 cut(s)	169, 1148, 2332, 3586
BsaXI	ACNNNNNCTCC	2 cut(s)	165, 195
Bsc4I	CCNNNNNNNGG	7 cut(s)	770, 1877, 2449, 2966, 2996, 3322, 3523
Bse118I	RCCGGY	2 cut(s)	326, 1148
Bse1I	ACTGG	9 cut(s)	427, 764, 1183, 1717, 2239, 2471, 2794, 3001, 3251
Bse3DI	GCAATG	1 cut(s)	1972
BseAI	TCCGGA	2 cut(s)	169, 2332
BseBI	CCWGG	5 cut(s)	13, 1566, 2271, 2444, 3198
BseCI	ATCGAT	1 cut(s)	207
BseLI	CCNNNNNNNGG	7 cut(s)	770, 1877, 2449, 2966, 2996, 3322, 3523
BseMI	GCAATG	1 cut(s)	1972
BseMII	CTCAG	5 cut(s)	734, 1473, 1748, 3386, 3766
BseNI	ACTGG	9 cut(s)	427, 764, 1183, 1717, 2239, 2471, 2794, 3001, 3251
BseRI	GAGGAG	3 cut(s)	13, 3361, 3689
BseYI	CCCAGC	1 cut(s)	2631
Bsh1236I	CGCG	3 cut(s)	36, 1453, 2532
BshNI	GGYRCC	2 cut(s)	1576, 1817
BshTI	ACCGGT	1 cut(s)	1148
BshVI	ATCGAT	1 cut(s)	207
BsiHKAI	GWGCWC	2 cut(s)	183, 3699
BsiSI	CCGG	7 cut(s)	170, 327, 1149, 1443, 2333, 2990, 3587
BslFI	GGGAC	5 cut(s)	812, 1881, 2248, 2958, 3600
BslI	CCNNNNNNNGG	7 cut(s)	770, 1877, 2449, 2966, 2996, 3322, 3523
BsmAI	GTCTC	6 cut(s)	165, 434, 974, 1330, 1748, 3753
BsmBI	CGTCTC	1 cut(s)	3753
BsmFI	GGGAC	5 cut(s)	812, 1881, 2248, 2958, 3600
BsmI	GAATGC	4 cut(s)	367, 399, 763, 2739
Bso31I	GGTCTC	1 cut(s)	165
Bsp119I	TTCGAA	2 cut(s)	377, 1332
Bsp1286I	GDGCHC	3 cut(s)	183, 1451, 3699
Bsp13I	TCCGGA	2 cut(s)	169, 2332
BspCNI	CTCAG	5 cut(s)	733, 1474, 1747, 3387, 3765
BspDI	ATCGAT	1 cut(s)	207
BspEI	TCCGGA	2 cut(s)	169, 2332
BspFNI	CGCG	3 cut(s)	36, 1453, 2532
BspMAI	CTGCAG	5 cut(s)	319, 556, 1848, 3508, 3602
BspMI	ACCTGC	4 cut(s)	886, 1466, 2147, 3643
BspPI	GGATC	6 cut(s)	180, 188, 249, 1713, 1726, 3739
BspT104I	TTCGAA	2 cut(s)	377, 1332
BspT107I	GGYRCC	2 cut(s)	1576, 1817
BspTI	CTTAAG	2 cut(s)	485, 2774
BspTNI	GGTCTC	1 cut(s)	165
BsrBI	CCGCTC	1 cut(s)	117
BsrDI	GCAATG	1 cut(s)	1972
BsrFI	RCCGGY	2 cut(s)	326, 1148
BsrI	ACTGG	9 cut(s)	427, 764, 1183, 1717, 2239, 2471, 2794, 3001, 3251
BssAI	RCCGGY	2 cut(s)	326, 1148
BssNI	GRCGYC	2 cut(s)	80, 3746
BssT1I	CCWWGG	7 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2871
Bst2UI	CCWGG	5 cut(s)	13, 1566, 2271, 2444, 3198
Bst4CI	ACNGT	5 cut(s)	445, 454, 542, 1726, 2949
Bst6I	CTCTTC	7 cut(s)	31, 930, 1139, 1560, 1863, 2180, 2922
BstACI	GRCGYC	2 cut(s)	80, 3746
BstAFI	CTTAAG	2 cut(s)	485, 2774
BstAPI	GCANNNNNTGC	2 cut(s)	323, 1658
BstBI	TTCGAA	2 cut(s)	377, 1332
BstC8I	GCNNGC	7 cut(s)	119, 126, 339, 889, 1451, 2067, 3012
BstEII	GGTNACC	1 cut(s)	2159
BstENI	CCTNNNNNAGG	2 cut(s)	2964, 3320
BstFNI	CGCG	3 cut(s)	36, 1453, 2532
BstH2I	RGCGCY	3 cut(s)	73, 2600, 2670
BstHHI	GCGC	8 cut(s)	18, 38, 72, 1455, 2534, 2599, 2669, 3646
BstMAI	GTCTC	6 cut(s)	165, 434, 974, 1330, 1748, 3753
BstNI	CCWGG	5 cut(s)	13, 1566, 2271, 2444, 3198
BstNSI	RCATGY	4 cut(s)	530, 677, 3178, 3644
BstPAI	GACNNNNGTC	1 cut(s)	3612
BstPI	GGTNACC	1 cut(s)	2159
BstSCI	CCNGG	7 cut(s)	11, 1442, 1564, 2269, 2442, 2989, 3196
BstSFI	CTRYAG	5 cut(s)	315, 552, 1844, 3504, 3598
BstUI	CGCG	3 cut(s)	36, 1453, 2532
BstV2I	GAAGAC	3 cut(s)	608, 2105, 3749
BstX2I	RGATCY	3 cut(s)	254, 1718, 2590
BstXI	CCANNNNNNTGG	1 cut(s)	247
BstYI	RGATCY	3 cut(s)	254, 1718, 2590
Bsu15I	ATCGAT	1 cut(s)	207
BsuI	GTATCC	2 cut(s)	1078, 1585
BsuTUI	ATCGAT	1 cut(s)	207
BtgZI	GCGATG	1 cut(s)	657
BtrI	CACGTC	1 cut(s)	3188
BtsI	GCAGTG	3 cut(s)	240, 1038, 1739
BtsIMutI	CAGTG	5 cut(s)	240, 450, 1038, 1722, 1739
BveI	ACCTGC	4 cut(s)	886, 1466, 2147, 3643
Cac8I	GCNNGC	7 cut(s)	119, 126, 339, 889, 1451, 2067, 3012
CaiI	CAGNNNCTG	4 cut(s)	694, 878, 1481, 1741
CfoI	GCGC	8 cut(s)	18, 38, 72, 1455, 2534, 2599, 2669, 3646
Cfr10I	RCCGGY	2 cut(s)	326, 1148
ClaI	ATCGAT	1 cut(s)	207
CseI	GACGC	2 cut(s)	69, 822
CspAI	ACCGGT	1 cut(s)	1148
CviAII	CATG	9 cut(s)	527, 674, 1658, 2357, 3175, 3206, 3238, 3641, 3647
DraI	TTTAAA	2 cut(s)	1279, 1789
DriI	GACNNNNNGTC	1 cut(s)	544
EaeI	YGGCCR	1 cut(s)	3408
Eam1104I	CTCTTC	7 cut(s)	31, 930, 1139, 1560, 1863, 2180, 2922
Eam1105I	GACNNNNNGTC	1 cut(s)	544
EarI	CTCTTC	7 cut(s)	31, 930, 1139, 1560, 1863, 2180, 2922
EciI	GGCGGA	5 cut(s)	34, 186, 1731, 3584, 3744
Ecl136II	GAGCTC	2 cut(s)	181, 3697
Eco130I	CCWWGG	7 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2871
Eco147I	AGGCCT	4 cut(s)	284, 1103, 2926, 3307
Eco24I	GRGCYC	3 cut(s)	183, 1451, 3699
Eco31I	GGTCTC	1 cut(s)	165
Eco32I	GATATC	1 cut(s)	1257
Eco47I	GGWCC	8 cut(s)	140, 490, 799, 1241, 1469, 1832, 1868, 2984
Eco47III	AGCGCT	2 cut(s)	71, 2598
Eco53kI	GAGCTC	2 cut(s)	181, 3697
Eco57I	CTGAAG	2 cut(s)	2903, 3051
Eco91I	GGTNACC	1 cut(s)	2159
EcoICRI	GAGCTC	2 cut(s)	181, 3697
EcoNI	CCTNNNNNAGG	2 cut(s)	2964, 3320
EcoO109I	RGGNCCY	4 cut(s)	490, 799, 1920, 2868
EcoO65I	GGTNACC	1 cut(s)	2159
EcoRI	GAATTC	2 cut(s)	373, 1299
EcoRII	CCWGG	5 cut(s)	11, 1564, 2269, 2442, 3196
EcoRV	GATATC	1 cut(s)	1257
EcoT14I	CCWWGG	7 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2871
EcoT22I	ATGCAT	4 cut(s)	679, 780, 2173, 2658
EcoT38I	GRGCYC	3 cut(s)	183, 1451, 3699
ErhI	CCWWGG	7 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2871
Esp3I	CGTCTC	1 cut(s)	3753
FaeI	CATG	9 cut(s)	530, 677, 1661, 2360, 3178, 3209, 3241, 3644, 3650
FalI	AAGNNNNNCTT	8 cut(s)	1884, 1916, 2101, 2133, 2282, 2314, 3058, 3090
FaqI	GGGAC	5 cut(s)	812, 1881, 2248, 2958, 3600
FatI	CATG	9 cut(s)	526, 673, 1657, 2356, 3174, 3205, 3237, 3640, 3646
FauI	CCCGC	3 cut(s)	110, 330, 1458
FauNDI	CATATG	2 cut(s)	780, 1876
FbaI	TGATCA	1 cut(s)	997
FblI	GTMKAC	2 cut(s)	2350, 3680
FriOI	GRGCYC	3 cut(s)	183, 1451, 3699
FspAI	RTGCGCAY	1 cut(s)	3645
FspBI	CTAG	9 cut(s)	560, 1158, 1263, 1533, 1692, 2016, 2594, 3129, 3144
FspI	TGCGCA	1 cut(s)	3645
GlaI	GCGC	8 cut(s)	17, 37, 71, 1454, 2533, 2598, 2668, 3645
GsaI	CCCAGC	1 cut(s)	2635
GsuI	CTGGAG	3 cut(s)	747, 945, 2064
HaeII	RGCGCY	3 cut(s)	73, 2600, 2670
HapII	CCGG	7 cut(s)	170, 327, 1149, 1443, 2333, 2990, 3587
HgaI	GACGC	2 cut(s)	69, 822
HhaI	GCGC	8 cut(s)	18, 38, 72, 1455, 2534, 2599, 2669, 3646
Hin1I	GRCGYC	2 cut(s)	80, 3746
Hin1II	CATG	9 cut(s)	530, 677, 1661, 2360, 3178, 3209, 3241, 3644, 3650
Hin6I	GCGC	8 cut(s)	16, 36, 70, 1453, 2532, 2597, 2667, 3644
HinP1I	GCGC	8 cut(s)	16, 36, 70, 1453, 2532, 2597, 2667, 3644
HincII	GTYRAC	4 cut(s)	2952, 3169, 3244, 3681
HindII	GTYRAC	4 cut(s)	2952, 3169, 3244, 3681
HindIII	AAGCTT	3 cut(s)	347, 2063, 2192
HpaI	GTTAAC	1 cut(s)	3244
HpaII	CCGG	7 cut(s)	170, 327, 1149, 1443, 2333, 2990, 3587
Hpy99I	CGWCG	3 cut(s)	100, 838, 896
HpyCH4III	ACNGT	5 cut(s)	445, 454, 542, 1726, 2949
HpyCH4IV	ACGT	7 cut(s)	1306, 2485, 3105, 3187, 3277, 3432, 3746
HpySE526I	ACGT	7 cut(s)	1306, 2485, 3105, 3187, 3277, 3432, 3746
Hsp92I	GRCGYC	2 cut(s)	80, 3746
Hsp92II	CATG	9 cut(s)	530, 677, 1661, 2360, 3178, 3209, 3241, 3644, 3650
HspAI	GCGC	8 cut(s)	16, 36, 70, 1453, 2532, 2597, 2667, 3644
KflI	GGGWCCC	1 cut(s)	799
Kpn2I	TCCGGA	2 cut(s)	169, 2332
Ksp22I	TGATCA	1 cut(s)	997
KspAI	GTTAAC	1 cut(s)	3244
LmnI	GCTCC	8 cut(s)	26, 114, 130, 186, 766, 1446, 2364, 3702
MaeI	CTAG	9 cut(s)	560, 1158, 1263, 1533, 1692, 2016, 2594, 3129, 3144
MaeII	ACGT	7 cut(s)	1306, 2485, 3105, 3187, 3277, 3432, 3746
MbiI	CCGCTC	1 cut(s)	117
MflI	RGATCY	3 cut(s)	254, 1718, 2590
MhlI	GDGCHC	3 cut(s)	183, 1451, 3699
MlsI	TGGCCA	1 cut(s)	3410
MluNI	TGGCCA	1 cut(s)	3410
MlyI	GAGTC	2 cut(s)	1785, 3686
MmeI	TCCRAC	2 cut(s)	2223, 2460
Mox20I	TGGCCA	1 cut(s)	3410
Mph1103I	ATGCAT	4 cut(s)	679, 780, 2173, 2658
MroI	TCCGGA	2 cut(s)	169, 2332
MroXI	GAANNNNTTC	2 cut(s)	1227, 1338
MscI	TGGCCA	1 cut(s)	3410
MslI	CAYNNNNRTG	5 cut(s)	623, 971, 1527, 3059, 3645
Msp20I	TGGCCA	1 cut(s)	3410
MspA1I	CMGCKG	5 cut(s)	128, 551, 694, 1843, 2437
MspCI	CTTAAG	2 cut(s)	485, 2774
MspI	CCGG	7 cut(s)	170, 327, 1149, 1443, 2333, 2990, 3587
MspR9I	CCNGG	7 cut(s)	13, 1444, 1566, 2271, 2444, 2991, 3198
Mva1269I	GAATGC	4 cut(s)	367, 399, 763, 2739
MvaI	CCWGG	5 cut(s)	13, 1566, 2271, 2444, 3198
MvnI	CGCG	3 cut(s)	36, 1453, 2532
NciI	CCSGG	2 cut(s)	1444, 2991
NdeI	CATATG	2 cut(s)	780, 1876
NlaIII	CATG	9 cut(s)	530, 677, 1661, 2360, 3178, 3209, 3241, 3644, 3650
NmuCI	GTSAC	6 cut(s)	536, 861, 1796, 2425, 2642, 3479
NsbI	TGCGCA	1 cut(s)	3645
NsiI	ATGCAT	4 cut(s)	679, 780, 2173, 2658
NspI	RCATGY	4 cut(s)	530, 677, 3178, 3644
NspV	TTCGAA	2 cut(s)	377, 1332
OliI	CACNNNNGTG	1 cut(s)	623
PaqCI	CACCTGC	1 cut(s)	3643
PceI	AGGCCT	4 cut(s)	284, 1103, 2926, 3307
PciI	ACATGT	1 cut(s)	526
PctI	GAATGC	4 cut(s)	367, 399, 763, 2739
PdmI	GAANNNNTTC	2 cut(s)	1227, 1338
PflFI	GACNNNGTC	1 cut(s)	1802
PflMI	CCANNNNNTGG	2 cut(s)	770, 1877
PinAI	ACCGGT	1 cut(s)	1148
PleI	GAGTC	2 cut(s)	1784, 3685
PpsI	GAGTC	2 cut(s)	1784, 3685
PpuMI	RGGWCCY	2 cut(s)	490, 799
PscI	ACATGT	1 cut(s)	526
PshAI	GACNNNNGTC	1 cut(s)	3612
PshBI	ATTAAT	1 cut(s)	993
Psp124BI	GAGCTC	2 cut(s)	183, 3699
Psp5II	RGGWCCY	2 cut(s)	490, 799
Psp6I	CCWGG	5 cut(s)	11, 1564, 2269, 2442, 3196
PspEI	GGTNACC	1 cut(s)	2159
PspFI	CCCAGC	1 cut(s)	2631
PspGI	CCWGG	5 cut(s)	11, 1564, 2269, 2442, 3196
PspPPI	RGGWCCY	2 cut(s)	490, 799
PstI	CTGCAG	5 cut(s)	319, 556, 1848, 3508, 3602
PstNI	CAGNNNCTG	4 cut(s)	694, 878, 1481, 1741
PsuI	RGATCY	3 cut(s)	254, 1718, 2590
PsyI	GACNNNGTC	1 cut(s)	1802
PvuII	CAGCTG	2 cut(s)	694, 2437
RseI	CAYNNNNRTG	5 cut(s)	623, 971, 1527, 3059, 3645
SacI	GAGCTC	2 cut(s)	183, 3699
SalI	GTCGAC	1 cut(s)	3679
ScaI	AGTACT	1 cut(s)	505
SchI	GAGTC	2 cut(s)	1785, 3686
ScrFI	CCNGG	7 cut(s)	13, 1444, 1566, 2271, 2444, 2991, 3198
SduI	GDGCHC	3 cut(s)	183, 1451, 3699
SfcI	CTRYAG	5 cut(s)	315, 552, 1844, 3504, 3598
SfuI	TTCGAA	2 cut(s)	377, 1332
SinI	GGWCC	8 cut(s)	140, 490, 799, 1241, 1469, 1832, 1868, 2984
SmiMI	CAYNNNNRTG	5 cut(s)	623, 971, 1527, 3059, 3645
SmlI	CTYRAG	4 cut(s)	176, 485, 2084, 2774
SmoI	CTYRAG	4 cut(s)	176, 485, 2084, 2774
SseBI	AGGCCT	4 cut(s)	284, 1103, 2926, 3307
SspI	AATATT	5 cut(s)	828, 991, 1021, 1424, 2518
SspMI	CTAG	9 cut(s)	560, 1158, 1263, 1533, 1692, 2016, 2594, 3129, 3144
SstI	GAGCTC	2 cut(s)	183, 3699
StuI	AGGCCT	4 cut(s)	284, 1103, 2926, 3307
StyD4I	CCNGG	7 cut(s)	11, 1442, 1564, 2269, 2442, 2989, 3196
StyI	CCWWGG	7 cut(s)	285, 1500, 1587, 1616, 2031, 2341, 2871
TaaI	ACNGT	5 cut(s)	445, 454, 542, 1726, 2949
TaiI	ACGT	7 cut(s)	1309, 2488, 3108, 3190, 3280, 3435, 3749
TaqI	TCGA	7 cut(s)	98, 207, 377, 940, 1332, 1885, 3680
TaqII	GACCGA	1 cut(s)	446
TatI	WGTACW	1 cut(s)	503
TauI	GCSGC	1 cut(s)	124
TscAI	CASTG	5 cut(s)	247, 457, 1045, 1729, 1746
TseFI	GTSAC	6 cut(s)	536, 861, 1796, 2425, 2642, 3479
Tsp45I	GTSAC	6 cut(s)	536, 861, 1796, 2425, 2642, 3479
TspDTI	ATGAA	9 cut(s)	81, 421, 989, 1062, 1419, 2402, 2811, 3226, 3239
TspGWI	ACGGA	2 cut(s)	120, 311
TspRI	CASTG	5 cut(s)	247, 457, 1045, 1729, 1746
Tth111I	GACNNNGTC	1 cut(s)	1802
Van91I	CCANNNNNTGG	2 cut(s)	770, 1877
Vha464I	CTTAAG	2 cut(s)	485, 2774
VpaK11BI	GGWCC	8 cut(s)	140, 490, 799, 1241, 1469, 1832, 1868, 2984
VspI	ATTAAT	1 cut(s)	993
XagI	CCTNNNNNAGG	2 cut(s)	2964, 3320
XapI	RAATTY	7 cut(s)	373, 1299, 1396, 1808, 2798, 3528, 3555
XceI	RCATGY	4 cut(s)	530, 677, 3178, 3644
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1103, 3418
XmiI	GTMKAC	2 cut(s)	2350, 3680
XmnI	GAANNNNTTC	2 cut(s)	1227, 1338
XspI	CTAG	9 cut(s)	560, 1158, 1263, 1533, 1692, 2016, 2594, 3129, 3144
ZraI	GACGTC	1 cut(s)	3747
ZrmI	AGTACT	1 cut(s)	505
Zsp2I	ATGCAT	4 cut(s)	679, 780, 2173, 2658

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.