Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0006621

Physical Location & Seq

Genomic Coordinates Forward (+)

29238543 .. 29240794

Gene Structure 2252 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 858 bp

ATGGCATTTAGTCCAGCGCAAATCCAGACTAACCGAAAAGCAAATCACGCCACCGAAGGCCGCCGCCGAATGACCTCCAAATCCATGGACCTCATGTCTCATTCCTCCGCTTTCCTCCTCCCTACGCTTCCGCCCAACCAGACGCCTTCTCCGACCTACGTGCTCGTCTTGCTCAACCAGAGCCTCCCGAGATTCGCTCCCCTGCTCTGGAACCACGCACAAATACGTATGTGTGCGGACGGCGGTGCCAATCGCGTTTACGATGACATGCCTCTTCTGCTGCCCCACGATAACGCCGTAGATGTTCGGAAAAGGTACAAGCCTGATGTTATTAAAGGCGACATGGATTCAATCCGAAAGGATGTCCTGGAATTTTATGCTAGTATGGGAACCCAAATTATTAATGAATCTGAGGATCAGGACACGACGGATCTGCATAAGTGTATAGCATACATATGTGACTCGGCACCAAACCTAGATAAATCTAATCTATGCATTCTGGTTGCTGGGGCACTTGGTGGACGGTTTGACCACGAGATTGGAAACATCAACATGTTATGTCGATTCTCAAACATTCGAATAATTCTCATATCTGATGATTGCCTCATCCATCTTCTTCCAAGAACACACTGCCATGAGATTCATATTCAGTCTTCTGCTGAAGGCCCTCATTGTGGACTCGTACCCATTGGGATTCCATCTGGAAGTACTACAACCAAGGGGCTTCAATGGGATCTCGCTGAAACAGAGATGAGGTTTGGTGGTCTGATAAGTACTTCAAATCTCGTCAAGGGAGAGAAAATAACAGTGCAATCCGATGCAGATCTTCTTTGGACTATAAGCATTAAAAAGACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004788 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009229 GO:0009987 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0034641 GO:0042357 GO:0042723 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0051186 GO:0051188 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

TPK_B1_binding TPK_catalytic

Protein Analysis

286

Amino Acids

31.6

Weight (kDa)

6.4

Isoelectric Point (pI)

51.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPK_catalytic	PF04263	68 - 195	1.9e-39	Thiamin pyrophosphokinase, catalytic domain
TPK_B1_binding	PF04265	212 - 277	4.9e-21	Thiamin pyrophosphokinase, vitamin B1 binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02880 AT1G02880 AT1G02880 AT2G44750 AT2G44750
fragaria_vesca	FvH4_2g25500 FvH4_2g25500
malus_domestica	MD02G1044000.v1.1 MD02G1044100.v1.1 MD03G1285900.v1.1 MD06G1023200.v1.1 MD06G1023300.v1.1 MD11G1034700.v1.1 MD11G1305700.v1.1
prunus_persica	Prupe.8G259100_v2.0.a1
pyrus_communis	pycom03g22660 pycom11g26750
rosa_chinensis	RchiOBHm_Chr6g0293711
rosa_laevigata	RLG00000011930
rosa_roxburghii	Rroxscaffold_7G00173970
rosa_rugosa	Rorug06G0237500
rosa_samantha	Rh6AG350300 Rh6BG357800 Rh6CG364600 Rh6DG351500
rosa_wichuraiana	Rw6G030500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	245, 466
AccII	CGCG	1 cut(s)	255
AciI	CCGC	6 cut(s)	61, 64, 108, 131, 236, 243
AclWI	GGATC	3 cut(s)	423, 438, 741
AcsI	RAATTY	1 cut(s)	371
AcuI	CTGAAG	1 cut(s)	681
AcyI	GRCGYC	1 cut(s)	143
AdeI	CACNNNGTG	1 cut(s)	518
AfaI	GTAC	4 cut(s)	317, 684, 709, 775
AfiI	CCNNNNNNNGG	2 cut(s)	207, 674
AflIII	ACRYGT	1 cut(s)	552
AgsI	TTSAA	3 cut(s)	351, 728, 780
AhdI	GACNNNNNGTC	1 cut(s)	94
AjnI	CCWGG	1 cut(s)	366
Alw21I	GWGCWC	1 cut(s)	165
Alw26I	GTCTC	1 cut(s)	102
AlwI	GGATC	3 cut(s)	423, 438, 741
Ama87I	CYCGRG	1 cut(s)	187
AoxI	GGCC	2 cut(s)	58, 664
ApeKI	GCWGC	1 cut(s)	280
ApoI	RAATTY	1 cut(s)	371
AseI	ATTAAT	1 cut(s)	402
AspLEI	GCGC	1 cut(s)	19
AspS9I	GGNCC	2 cut(s)	88, 665
AsuII	TTCGAA	1 cut(s)	577
AvaI	CYCGRG	1 cut(s)	187
AvaII	GGWCC	1 cut(s)	88
BaeGI	GKGCMC	1 cut(s)	514
BanI	GGYRCC	2 cut(s)	245, 466
BauI	CACGAG	1 cut(s)	533
BbsI	GAAGAC	1 cut(s)	645
Bbv12I	GWGCWC	1 cut(s)	165
BbvI	GCAGC	1 cut(s)	267
BccI	CCATC	2 cut(s)	618, 706
BceAI	ACGGC	2 cut(s)	256, 281
BcgI	CGANNNNNNTGC	4 cut(s)	142, 176, 415, 449
BciT130I	CCWGG	1 cut(s)	368
BcoDI	GTCTC	1 cut(s)	102
BfaI	CTAG	2 cut(s)	381, 476
BglII	AGATCT	1 cut(s)	823
BisI	GCNGC	3 cut(s)	61, 64, 281
BlsI	GCNGC	3 cut(s)	62, 65, 282
BmcAI	AGTACT	2 cut(s)	709, 775
Bme1390I	CCNGG	1 cut(s)	368
Bme18I	GGWCC	1 cut(s)	88
BmeRI	GACNNNNNGTC	1 cut(s)	94
BmeT110I	CYCGRG	1 cut(s)	187
BmgT120I	GGNCC	2 cut(s)	88, 665
BmiI	GGNNCC	4 cut(s)	212, 247, 391, 468
BmrFI	CCNGG	1 cut(s)	368
BmsI	GCATC	1 cut(s)	808
BpiI	GAAGAC	1 cut(s)	645
BplI	GAGNNNNNCTC	2 cut(s)	181, 213
Bpu14I	TTCGAA	1 cut(s)	577
BsaAI	YACGTR	2 cut(s)	160, 227
BsaBI	GATNNNNATC	1 cut(s)	822
BsaHI	GRCGYC	1 cut(s)	143
BsaJI	CCNNGG	2 cut(s)	84, 717
BsaXI	ACNNNNNCTCC	2 cut(s)	133, 163
Bsc4I	CCNNNNNNNGG	2 cut(s)	207, 674
Bse8I	GATNNNNATC	1 cut(s)	822
BseBI	CCWGG	1 cut(s)	368
BseDI	CCNNGG	2 cut(s)	84, 717
BseGI	GGATG	2 cut(s)	367, 606
BseJI	GATNNNNATC	1 cut(s)	822
BseLI	CCNNNNNNNGG	2 cut(s)	207, 674
BseMII	CTCAG	1 cut(s)	402
BseRI	GAGGAG	1 cut(s)	107
BseSI	GKGCMC	1 cut(s)	514
BseXI	GCAGC	1 cut(s)	267
BseYI	CCCAGC	1 cut(s)	506
Bsh1236I	CGCG	1 cut(s)	255
BshFI	GGCC	2 cut(s)	60, 666
BshNI	GGYRCC	2 cut(s)	245, 466
BsiHKAI	GWGCWC	1 cut(s)	165
BsiHKCI	CYCGRG	1 cut(s)	187
BslI	CCNNNNNNNGG	2 cut(s)	207, 674
BsmAI	GTCTC	1 cut(s)	102
BsmI	GAATGC	1 cut(s)	495
BsnI	GGCC	2 cut(s)	60, 666
BsoBI	CYCGRG	1 cut(s)	187
Bsp119I	TTCGAA	1 cut(s)	577
Bsp1286I	GDGCHC	2 cut(s)	165, 514
Bsp143I	GATC	4 cut(s)	415, 430, 733, 823
Bsp19I	CCATGG	1 cut(s)	84
BspACI	CCGC	6 cut(s)	61, 64, 108, 131, 236, 243
BspANI	GGCC	2 cut(s)	60, 666
BspCNI	CTCAG	1 cut(s)	403
BspFNI	CGCG	1 cut(s)	255
BspLI	GGNNCC	4 cut(s)	212, 247, 391, 468
BspPI	GGATC	3 cut(s)	423, 438, 741
BspT104I	TTCGAA	1 cut(s)	577
BspT107I	GGYRCC	2 cut(s)	245, 466
BssECI	CCNNGG	2 cut(s)	84, 717
BssMI	GATC	4 cut(s)	415, 430, 733, 823
BssNI	GRCGYC	1 cut(s)	143
BssSI	CACGAG	1 cut(s)	533
BssT1I	CCWWGG	2 cut(s)	84, 717
Bst2BI	CACGAG	1 cut(s)	533
Bst2UI	CCWGG	1 cut(s)	368
Bst4CI	ACNGT	2 cut(s)	525, 808
Bst6I	CTCTTC	1 cut(s)	279
BstACI	GRCGYC	1 cut(s)	143
BstBAI	YACGTR	2 cut(s)	160, 227
BstBI	TTCGAA	1 cut(s)	577
BstDEI	CTNAG	1 cut(s)	411
BstDSI	CCRYGG	1 cut(s)	84
BstF5I	GGATG	2 cut(s)	367, 606
BstFNI	CGCG	1 cut(s)	255
BstHHI	GCGC	1 cut(s)	19
BstKTI	GATC	4 cut(s)	418, 433, 736, 826
BstMAI	GTCTC	1 cut(s)	102
BstMBI	GATC	4 cut(s)	415, 430, 733, 823
BstMWI	GCNNNNNNNGC	3 cut(s)	47, 169, 277
BstNI	CCWGG	1 cut(s)	368
BstNSI	RCATGY	2 cut(s)	271, 556
BstSCI	CCNGG	1 cut(s)	366
BstSLI	GKGCMC	1 cut(s)	514
BstSNI	TACGTA	1 cut(s)	227
BstUI	CGCG	1 cut(s)	255
BstV1I	GCAGC	1 cut(s)	267
BstV2I	GAAGAC	1 cut(s)	645
BstX2I	RGATCY	3 cut(s)	430, 733, 823
BstXI	CCANNNNNNTGG	2 cut(s)	85, 539
BstYI	RGATCY	3 cut(s)	430, 733, 823
BsuRI	GGCC	2 cut(s)	60, 666
BtgI	CCRYGG	1 cut(s)	84
BtsCI	GGATG	2 cut(s)	367, 606
BtsI	GCAGTG	1 cut(s)	628
BtsIMutI	CAGTG	2 cut(s)	628, 813
CfoI	GCGC	1 cut(s)	19
Cfr13I	GGNCC	2 cut(s)	88, 665
CseI	GACGC	1 cut(s)	151
Csp6I	GTAC	4 cut(s)	316, 683, 708, 774
CviAII	CATG	6 cut(s)	85, 94, 268, 343, 553, 635
CviJI	RGCY	5 cut(s)	60, 183, 322, 666, 724
CviKI_1	RGCY	5 cut(s)	60, 183, 322, 666, 724
CviQI	GTAC	4 cut(s)	316, 683, 708, 774
DdeI	CTNAG	1 cut(s)	411
DpnI	GATC	4 cut(s)	417, 432, 735, 825
DpnII	GATC	4 cut(s)	415, 430, 733, 823
DraIII	CACNNNGTG	1 cut(s)	518
DriI	GACNNNNNGTC	1 cut(s)	94
Eam1104I	CTCTTC	1 cut(s)	279
Eam1105I	GACNNNNNGTC	1 cut(s)	94
EarI	CTCTTC	1 cut(s)	279
EciI	GGCGGA	1 cut(s)	120
Eco105I	TACGTA	1 cut(s)	227
Eco130I	CCWWGG	2 cut(s)	84, 717
Eco47I	GGWCC	1 cut(s)	88
Eco57I	CTGAAG	1 cut(s)	681
Eco88I	CYCGRG	1 cut(s)	187
EcoO109I	RGGNCCY	1 cut(s)	665
EcoRII	CCWGG	1 cut(s)	366
EcoT14I	CCWWGG	2 cut(s)	84, 717
EcoT22I	ATGCAT	1 cut(s)	497
ErhI	CCWWGG	2 cut(s)	84, 717
FaeI	CATG	6 cut(s)	88, 97, 271, 346, 556, 638
FatI	CATG	6 cut(s)	84, 93, 267, 342, 552, 634
FauNDI	CATATG	1 cut(s)	455
Fnu4HI	GCNGC	3 cut(s)	61, 64, 281
FokI	GGATG	2 cut(s)	374, 593
Fsp4HI	GCNGC	3 cut(s)	61, 64, 281
FspBI	CTAG	2 cut(s)	381, 476
GlaI	GCGC	1 cut(s)	18
GluI	GCNGC	3 cut(s)	61, 64, 281
GsaI	CCCAGC	1 cut(s)	510
HaeIII	GGCC	2 cut(s)	60, 666
HgaI	GACGC	1 cut(s)	151
HhaI	GCGC	1 cut(s)	19
Hin1I	GRCGYC	1 cut(s)	143
Hin1II	CATG	6 cut(s)	88, 97, 271, 346, 556, 638
Hin6I	GCGC	1 cut(s)	17
HinP1I	GCGC	1 cut(s)	17
HinfI	GANTC	8 cut(s)	192, 347, 407, 461, 564, 640, 678, 694
Hpy166II	GTNNAC	3 cut(s)	259, 521, 677
Hpy188I	TCNGA	7 cut(s)	153, 309, 356, 412, 595, 768, 817
Hpy188III	TCNNGA	5 cut(s)	25, 187, 208, 419, 702
Hpy8I	GTNNAC	3 cut(s)	259, 521, 677
Hpy99I	CGWCG	1 cut(s)	430
HpyAV	CCTTC	3 cut(s)	50, 156, 656
HpyCH4III	ACNGT	2 cut(s)	525, 808
HpyCH4IV	ACGT	3 cut(s)	159, 226, 854
HpyCH4V	TGCA	4 cut(s)	436, 495, 811, 821
HpyF10VI	GCNNNNNNNGC	3 cut(s)	47, 169, 277
HpyF3I	CTNAG	1 cut(s)	411
HpySE526I	ACGT	3 cut(s)	159, 226, 854
Hsp92I	GRCGYC	1 cut(s)	143
Hsp92II	CATG	6 cut(s)	88, 97, 271, 346, 556, 638
HspAI	GCGC	1 cut(s)	17
Kzo9I	GATC	4 cut(s)	415, 430, 733, 823
LmnI	GCTCC	1 cut(s)	202
Lsp1109I	GCAGC	1 cut(s)	267
LweI	GCATC	1 cut(s)	808
MaeI	CTAG	2 cut(s)	381, 476
MaeII	ACGT	3 cut(s)	159, 226, 854
MaeIII	GTNAC	1 cut(s)	458
MalI	GATC	4 cut(s)	417, 432, 735, 825
MboI	GATC	4 cut(s)	415, 430, 733, 823
MboII	GAAGA	5 cut(s)	266, 605, 608, 645, 818
MflI	RGATCY	3 cut(s)	430, 733, 823
MhlI	GDGCHC	2 cut(s)	165, 514
MluCI	AATT	3 cut(s)	371, 396, 582
MlyI	GAGTC	2 cut(s)	455, 672
MmeI	TCCRAC	1 cut(s)	176
Mph1103I	ATGCAT	1 cut(s)	497
MseI	TTAA	3 cut(s)	333, 402, 846
MslI	CAYNNNNRTG	3 cut(s)	454, 551, 633
MspR9I	CCNGG	1 cut(s)	368
Mva1269I	GAATGC	1 cut(s)	495
MvaI	CCWGG	1 cut(s)	368
MvnI	CGCG	1 cut(s)	255
MwoI	GCNNNNNNNGC	3 cut(s)	47, 169, 277
NcoI	CCATGG	1 cut(s)	84
NdeI	CATATG	1 cut(s)	455
NdeII	GATC	4 cut(s)	415, 430, 733, 823
NlaIII	CATG	6 cut(s)	88, 97, 271, 346, 556, 638
NlaIV	GGNNCC	4 cut(s)	212, 247, 391, 468
NmeAIII	GCCGAG	1 cut(s)	443
NmuCI	GTSAC	1 cut(s)	458
NsiI	ATGCAT	1 cut(s)	497
NspI	RCATGY	2 cut(s)	271, 556
NspV	TTCGAA	1 cut(s)	577
PciI	ACATGT	1 cut(s)	552
PcsI	WCGNNNNNNNCGW	2 cut(s)	149, 294
PctI	GAATGC	1 cut(s)	495
PfeI	GAWTC	6 cut(s)	192, 347, 407, 564, 640, 694
PfoI	TCCNGGA	1 cut(s)	366
PkrI	GCNGC	3 cut(s)	62, 65, 282
PleI	GAGTC	2 cut(s)	455, 672
PpsI	GAGTC	2 cut(s)	455, 672
Ppu21I	YACGTR	2 cut(s)	160, 227
PscI	ACATGT	1 cut(s)	552
PshBI	ATTAAT	1 cut(s)	402
Psp6I	CCWGG	1 cut(s)	366
PspFI	CCCAGC	1 cut(s)	506
PspGI	CCWGG	1 cut(s)	366
PspN4I	GGNNCC	4 cut(s)	212, 247, 391, 468
PspPI	GGNCC	2 cut(s)	88, 665
PsuI	RGATCY	3 cut(s)	430, 733, 823
RsaI	GTAC	4 cut(s)	317, 684, 709, 775
RsaNI	GTAC	4 cut(s)	316, 683, 708, 774
RseI	CAYNNNNRTG	3 cut(s)	454, 551, 633
SaqAI	TTAA	3 cut(s)	333, 402, 846
SatI	GCNGC	3 cut(s)	61, 64, 281
Sau3AI	GATC	4 cut(s)	415, 430, 733, 823
Sau96I	GGNCC	2 cut(s)	88, 665
ScaI	AGTACT	2 cut(s)	709, 775
SchI	GAGTC	2 cut(s)	455, 672
ScrFI	CCNGG	1 cut(s)	368
SduI	GDGCHC	2 cut(s)	165, 514
SetI	ASST	9 cut(s)	77, 93, 158, 162, 229, 317, 477, 758, 857
SfaNI	GCATC	1 cut(s)	808
SfuI	TTCGAA	1 cut(s)	577
SinI	GGWCC	1 cut(s)	88
SmiMI	CAYNNNNRTG	3 cut(s)	454, 551, 633
SnaBI	TACGTA	1 cut(s)	227
Sse9I	AATT	3 cut(s)	371, 396, 582
SsiI	CCGC	6 cut(s)	61, 64, 108, 131, 236, 243
SspMI	CTAG	2 cut(s)	381, 476
StyD4I	CCNGG	1 cut(s)	366
StyI	CCWWGG	2 cut(s)	84, 717
TaaI	ACNGT	2 cut(s)	525, 808
TaiI	ACGT	3 cut(s)	162, 229, 857
TaqI	TCGA	2 cut(s)	562, 577
TasI	AATT	3 cut(s)	371, 396, 582
TatI	WGTACW	2 cut(s)	707, 773
TauI	GCSGC	2 cut(s)	63, 66
TfiI	GAWTC	6 cut(s)	192, 347, 407, 564, 640, 694
Tru1I	TTAA	3 cut(s)	333, 402, 846
Tru9I	TTAA	3 cut(s)	333, 402, 846
TscAI	CASTG	2 cut(s)	635, 813
TseFI	GTSAC	1 cut(s)	458
TseI	GCWGC	1 cut(s)	280
Tsp45I	GTSAC	1 cut(s)	458
TspDTI	ATGAA	2 cut(s)	420, 632
TspGWI	ACGGA	1 cut(s)	443
TspRI	CASTG	2 cut(s)	635, 813
VpaK11BI	GGWCC	1 cut(s)	88
VspI	ATTAAT	1 cut(s)	402
XapI	RAATTY	1 cut(s)	371
XceI	RCATGY	2 cut(s)	271, 556
XspI	CTAG	2 cut(s)	381, 476
ZrmI	AGTACT	2 cut(s)	709, 775
Zsp2I	ATGCAT	1 cut(s)	497

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.