pycom12g00760

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008450
Physical Location & Seq
Forward (+)
673714 .. 675649
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00760.1

Sequence Viewer

Length: 807 bp
ATGTTCCTCCAGAGGCAGTGCGCCGCCGTCCTCACCCAAGCACTCCAAGTCCAACTCCGCCGCTACACCCAATCATCCGCCGCCGCCCTCCTCCACCCGGACCCCGAACTCGACCGCGACCTCGAATCCTCCACATCCTTCCCGATACCCGACCCGAAGTACGCCGAGACCATCTTCGCAGTCCCACGCACCACCTCCGGCAAGAGCATCGCCGCCAAAGAGCGCAAGGCCGGCCGCGTCCCCAGCATCATTTTCGAGCAGGAGGACGGCCAGCACGGCGGCAACAAGCGCCTCGTTTCTGTGCGGACCAACCAGATTCGCAAGCTCGTTGGTCATCTCGGCCGCTCTTTCTTCCTCTCCAGGCTCTTCGACCTCGAGGTTCGCTCCGACTTCGACTCTGAAAACGACGTCGTTGAACGGGTCCGCGTTTTGCCTCGCTCGATTCATCTGCACTCTGCGACGGACGCACCGCTGAATGTGACCTTCATACGGGCTCCTTCTCATGCTTTGTTGAAAGTCGAGATTCCCCTTGTGTTTCGAGGAGACGATGTCTCTCCTGGATTGAAGAAAAATGGTTGTCTAAATACGATCAAGAGGACCGTAAAGTTCCTTTGTCCTGCTGATGTGATTCCTCCATATATTGATGTGGATCTGAGCGAGTTGGATGTTAACCAAAAGATAGTAATGGGAGATCTCAAAGTTCATCCAGATCTCAAGCTTCTCCAGTCAAAGGATGAGCCTGTTTGTAAGATCACGGGAGCAAGGGTTTCTGAACAAAAGAAGTCTAAAGACAAAGACTCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

269

Amino Acids

29.93

Weight (kDa)

9.23

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L25p PF01386 60 - 162 1.8e-09 Ribosomal L25p family
Ribosomal_TL5_C PF14693 171 - 254 1.4e-20 Ribosomal protein TL5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 411
AbsI CCTCGAGG 1 cut(s) 374
AccBSI CCGCTC 1 cut(s) 345
AccII CGCG 3 cut(s) 117, 237, 426
AclWI GGATC 1 cut(s) 657
AcoI YGGCCR 3 cut(s) 232, 268, 340
AcyI GRCGYC 1 cut(s) 408
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 3 cut(s) 97, 489, 730
AgsI TTSAA 3 cut(s) 416, 514, 565
AjnI CCWGG 2 cut(s) 359, 556
AluBI AGCT 2 cut(s) 325, 718
AluI AGCT 2 cut(s) 325, 718
Alw26I GTCTC 3 cut(s) 161, 537, 556
AlwI GGATC 1 cut(s) 657
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 4 cut(s) 228, 232, 268, 340
AspLEI GCGC 3 cut(s) 23, 225, 291
AspS9I GGNCC 4 cut(s) 100, 306, 421, 597
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 4 cut(s) 100, 306, 421, 597
BanII GRGCYC 1 cut(s) 496
BccI CCATC 1 cut(s) 179
BceAI ACGGC 3 cut(s) 11, 283, 292
BcgI CGANNNNNNTGC 6 cut(s) 190, 224, 235, 269, 430, 464
BciT130I CCWGG 2 cut(s) 361, 558
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 3 cut(s) 161, 537, 556
BfoI RGCGCY 1 cut(s) 292
BglI GCCNNNNNGGC 1 cut(s) 276
BglII AGATCT 2 cut(s) 691, 709
BisI GCNGC 8 cut(s) 24, 61, 81, 84, 213, 235, 280, 343
BlsI GCNGC 8 cut(s) 25, 62, 82, 85, 214, 236, 281, 344
Bme1390I CCNGG 3 cut(s) 98, 361, 558
Bme18I GGWCC 4 cut(s) 100, 306, 421, 597
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 4 cut(s) 100, 306, 421, 597
BmiI GGNNCC 3 cut(s) 102, 422, 495
BmrFI CCNGG 3 cut(s) 98, 361, 558
BmsI GCATC 2 cut(s) 216, 255
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpmI CTGGAG 2 cut(s) 343, 707
BpuEI CTTGAG 1 cut(s) 698
BpuMI CCSGG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 648
BsaHI GRCGYC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 161
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 750, 780
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 489, 730
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 724
Bse8I GATNNNNATC 1 cut(s) 648
BseBI CCWGG 2 cut(s) 361, 558
BseGI GGATG 5 cut(s) 74, 134, 670, 703, 739
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 3 cut(s) 97, 489, 730
BseMII CTCAG 1 cut(s) 644
BseNI ACTGG 1 cut(s) 724
BseRI GAGGAG 2 cut(s) 80, 555
BseX3I CGGCCG 2 cut(s) 232, 340
BseYI CCCAGC 1 cut(s) 242
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 3 cut(s) 117, 237, 426
Bsh1285I CGRYCG 3 cut(s) 115, 235, 343
BshFI GGCC 4 cut(s) 230, 234, 270, 342
BsiEI CGRYCG 3 cut(s) 115, 235, 343
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 3 cut(s) 98, 198, 231
BslFI GGGAC 2 cut(s) 167, 224
BslI CCNNNNNNNGG 3 cut(s) 97, 489, 730
BsmAI GTCTC 3 cut(s) 161, 537, 556
BsmBI CGTCTC 1 cut(s) 537
BsmFI GGGAC 2 cut(s) 167, 224
BsnI GGCC 4 cut(s) 230, 234, 270, 342
Bso31I GGTCTC 1 cut(s) 161
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 496
Bsp143I GATC 5 cut(s) 588, 649, 691, 709, 750
BspANI GGCC 4 cut(s) 230, 234, 270, 342
BspCNI CTCAG 1 cut(s) 645
BspFNI CGCG 3 cut(s) 117, 237, 426
BspLI GGNNCC 3 cut(s) 102, 422, 495
BspPI GGATC 1 cut(s) 657
BspQI GCTCTTC 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 345
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 1 cut(s) 724
BssAI RCCGGY 1 cut(s) 230
BssMI GATC 5 cut(s) 588, 649, 691, 709, 750
BssNI GRCGYC 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 361, 558
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 1 cut(s) 371
BstACI GRCGYC 1 cut(s) 408
BstC8I GCNNGC 3 cut(s) 232, 272, 323
BstDEI CTNAG 1 cut(s) 653
BstF5I GGATG 5 cut(s) 74, 134, 670, 703, 739
BstFNI CGCG 3 cut(s) 117, 237, 426
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 3 cut(s) 23, 225, 291
BstKTI GATC 5 cut(s) 591, 652, 694, 712, 753
BstMAI GTCTC 3 cut(s) 161, 537, 556
BstMBI GATC 5 cut(s) 588, 649, 691, 709, 750
BstMCI CGRYCG 3 cut(s) 115, 235, 343
BstMWI GCNNNNNNNGC 5 cut(s) 231, 243, 276, 288, 464
BstNI CCWGG 2 cut(s) 361, 558
BstSCI CCNGG 3 cut(s) 96, 359, 556
BstUI CGCG 3 cut(s) 117, 237, 426
BstX2I RGATCY 3 cut(s) 649, 691, 709
BstYI RGATCY 3 cut(s) 649, 691, 709
BstZI CGGCCG 2 cut(s) 232, 340
BsuRI GGCC 4 cut(s) 230, 234, 270, 342
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 5 cut(s) 74, 134, 670, 703, 739
BtsI GCAGTG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 23
Cac8I GCNNGC 3 cut(s) 232, 272, 323
CfoI GCGC 3 cut(s) 23, 225, 291
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 4 cut(s) 100, 306, 421, 597
CseI GACGC 2 cut(s) 226, 473
Csp6I GTAC 1 cut(s) 160
CviAII CATG 1 cut(s) 503
CviJI RGCY 9 cut(s) 230, 234, 270, 325, 342, 364, 494, 718, 739
CviKI_1 RGCY 9 cut(s) 230, 234, 270, 325, 342, 364, 494, 718, 739
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 653
DpnI GATC 5 cut(s) 590, 651, 693, 711, 752
DpnII GATC 5 cut(s) 588, 649, 691, 709, 750
EaeI YGGCCR 3 cut(s) 232, 268, 340
EagI CGGCCG 2 cut(s) 232, 340
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
EciI GGCGGA 2 cut(s) 47, 67
EclXI CGGCCG 2 cut(s) 232, 340
Eco24I GRGCYC 1 cut(s) 496
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 4 cut(s) 100, 306, 421, 597
Eco52I CGGCCG 2 cut(s) 232, 340
Eco88I CYCGRG 1 cut(s) 374
EcoRII CCWGG 2 cut(s) 359, 556
EcoT38I GRGCYC 1 cut(s) 496
Esp3I CGTCTC 1 cut(s) 537
FaeI CATG 1 cut(s) 506
FaiI YATR 4 cut(s) 488, 504, 637, 639
FaqI GGGAC 2 cut(s) 167, 224
FatI CATG 1 cut(s) 502
Fnu4HI GCNGC 8 cut(s) 24, 61, 81, 84, 213, 235, 280, 343
FokI GGATG 5 cut(s) 61, 121, 677, 690, 746
FriOI GRGCYC 1 cut(s) 496
FseI GGCCGGCC 1 cut(s) 234
Fsp4HI GCNGC 8 cut(s) 24, 61, 81, 84, 213, 235, 280, 343
GlaI GCGC 3 cut(s) 22, 224, 290
GluI GCNGC 8 cut(s) 24, 61, 81, 84, 213, 235, 280, 343
GsaI CCCAGC 1 cut(s) 246
GsuI CTGGAG 2 cut(s) 343, 707
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 4 cut(s) 230, 234, 270, 342
HapII CCGG 3 cut(s) 98, 198, 231
HgaI GACGC 2 cut(s) 226, 473
HhaI GCGC 3 cut(s) 23, 225, 291
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 1 cut(s) 506
Hin6I GCGC 3 cut(s) 21, 223, 289
HinP1I GCGC 3 cut(s) 21, 223, 289
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 7 cut(s) 125, 316, 395, 442, 523, 628, 797
HpaI GTTAAC 1 cut(s) 670
HpaII CCGG 3 cut(s) 98, 198, 231
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 4 cut(s) 388, 400, 654, 772
Hpy188III TCNNGA 5 cut(s) 10, 142, 520, 592, 707
Hpy8I GTNNAC 1 cut(s) 670
Hpy99I CGWCG 3 cut(s) 410, 413, 463
HpyAV CCTTC 3 cut(s) 148, 493, 507
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 1 cut(s) 451
HpyF10VI GCNNNNNNNGC 5 cut(s) 231, 243, 276, 288, 464
HpyF3I CTNAG 1 cut(s) 653
HpySE526I ACGT 1 cut(s) 408
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 1 cut(s) 506
HspAI GCGC 3 cut(s) 21, 223, 289
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
KspAI GTTAAC 1 cut(s) 670
Kzo9I GATC 5 cut(s) 588, 649, 691, 709, 750
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 3 cut(s) 389, 499, 758
LweI GCATC 2 cut(s) 216, 255
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 1 cut(s) 478
MalI GATC 5 cut(s) 590, 651, 693, 711, 752
MbiI CCGCTC 1 cut(s) 345
MboI GATC 5 cut(s) 588, 649, 691, 709, 750
MboII GAAGA 4 cut(s) 166, 343, 358, 577
MflI RGATCY 3 cut(s) 649, 691, 709
MhlI GDGCHC 1 cut(s) 496
MlyI GAGTC 2 cut(s) 389, 791
MmeI TCCRAC 3 cut(s) 76, 411, 642
MroNI GCCGGC 1 cut(s) 230
MseI TTAA 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 472
MspI CCGG 3 cut(s) 98, 198, 231
MspR9I CCNGG 3 cut(s) 98, 361, 558
MvaI CCWGG 2 cut(s) 361, 558
MvnI CGCG 3 cut(s) 117, 237, 426
MwoI GCNNNNNNNGC 5 cut(s) 231, 243, 276, 288, 464
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 1 cut(s) 98
NdeII GATC 5 cut(s) 588, 649, 691, 709, 750
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 1 cut(s) 506
NlaIV GGNNCC 3 cut(s) 102, 422, 495
NmeAIII GCCGAG 2 cut(s) 190, 318
NmuCI GTSAC 1 cut(s) 478
PaeR7I CTCGAG 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 371
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 5 cut(s) 125, 316, 442, 523, 628
PflFI GACNNNGTC 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 556
PkrI GCNGC 8 cut(s) 25, 62, 82, 85, 214, 236, 281, 344
PleI GAGTC 2 cut(s) 389, 791
PpsI GAGTC 2 cut(s) 389, 791
Psp6I CCWGG 2 cut(s) 359, 556
PspFI CCCAGC 1 cut(s) 242
PspGI CCWGG 2 cut(s) 359, 556
PspN4I GGNNCC 3 cut(s) 102, 422, 495
PspPI GGNCC 4 cut(s) 100, 306, 421, 597
PspXI VCTCGAGB 1 cut(s) 374
PsuI RGATCY 3 cut(s) 649, 691, 709
PsyI GACNNNGTC 1 cut(s) 548
RigI GGCCGGCC 1 cut(s) 234
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 1 cut(s) 669
SatI GCNGC 8 cut(s) 24, 61, 81, 84, 213, 235, 280, 343
Sau3AI GATC 5 cut(s) 588, 649, 691, 709, 750
Sau96I GGNCC 4 cut(s) 100, 306, 421, 597
SchI GAGTC 2 cut(s) 389, 791
ScrFI CCNGG 3 cut(s) 98, 361, 558
SduI GDGCHC 1 cut(s) 496
SetI ASST 8 cut(s) 123, 197, 327, 375, 381, 411, 485, 720
SfaNI GCATC 2 cut(s) 216, 255
Sfr274I CTCGAG 1 cut(s) 374
SinI GGWCC 4 cut(s) 100, 306, 421, 597
SlaI CTCGAG 1 cut(s) 374
SmlI CTYRAG 2 cut(s) 374, 713
SmoI CTYRAG 2 cut(s) 374, 713
StyD4I CCNGG 3 cut(s) 96, 359, 556
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 1 cut(s) 411
TaqI TCGA 9 cut(s) 111, 123, 255, 369, 375, 393, 440, 519, 538
TauI GCSGC 8 cut(s) 26, 63, 83, 86, 215, 237, 282, 345
TfiI GAWTC 5 cut(s) 125, 316, 442, 523, 628
Tru1I TTAA 1 cut(s) 669
Tru9I TTAA 1 cut(s) 669
TscAI CASTG 1 cut(s) 23
TseFI GTSAC 1 cut(s) 478
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 3 cut(s) 434, 475, 692
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 1 cut(s) 23
Tth111I GACNNNGTC 1 cut(s) 548
VpaK11BI GGWCC 4 cut(s) 100, 306, 421, 597
XhoI CTCGAG 1 cut(s) 374
ZraI GACGTC 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.