RLG00000008657

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008450
Physical Location & Seq
Forward (+)
34317029 .. 34319405
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008657

Sequence Viewer

Length: 792 bp
ATGTTCCTCCTCCAGCGGCGTTGCGCCTCCGCACTCTCCTCACCCCTCCAGCTCCGCCGCTACGCCCAATCCGCCGCAGCCGCTCTCGTCGACGACGATTCCAACCCGGAGTCCTTCCCCCGACCCGACCCGAAATACGACGAGACCATCCTTGCCGTCCCGCGCTTAACCTCCGGCAAAAGCATCGCCGCCAAAGAGCGGAAGGCCGGCCGCGTCCCCGGCATCATCTTCGAGCAAGAGGACGGCCAGCACGGCGGCAATAAGCGACTCATCTCCGTCCGCACGAACCAGATCCGCAAGCTCCTCAATCATCTCGGCCGCTCTTTCTTCCTCTCCCGCCTCTTCCACCTCGAGGTCCGCTCCGACTTCGATGCAGACGACGTCGTTGAGAAAGTCCGCGTTTTACCGCGCTTGATTCATCTGCACTCGAGCACCGATGCTCCGCTTAATGTGACCTTCATAAGGGCCCCTTCAAATGCTTTGTTGAAAGTCGAAATTCCTCTTGTATTCAGAGGAGATGATATCTCTCCTGGATTGAAGAAAGGTGCATATTTAAATACAATCAAGAGGACGGTAAAGTTCCTATGCCCTGCGGATGTAATTCCTCCATATATTGATGTGGATCTGAGTGAGTTGGATGTTGGCCAAAAGCTAGTTATGGGAGACCTCAAGTTGCCCATTGGCATAAAATTCTACTGCCTCCAGCTCCTTCTCAGCTCAAATGTTGACATGTTCAACCCCTATATCTTGATCTGTAATGGCTGTAGAGGACTATGGGATGGAATCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

264

Amino Acids

29.34

Weight (kDa)

9.08

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L25p PF01386 52 - 153 2.9e-09 Ribosomal L25p family
Ribosomal_TL5_C PF14693 162 - 231 2.7e-17 Ribosomal protein TL5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
AbsI CCTCGAGG 1 cut(s) 350
AccBSI CCGCTC 4 cut(s) 83, 199, 321, 360
AccI GTMKAC 1 cut(s) 90
AccII CGCG 4 cut(s) 163, 213, 399, 409
AclWI GGATC 2 cut(s) 286, 630
AcoI YGGCCR 4 cut(s) 208, 244, 316, 643
AcsI RAATTY 2 cut(s) 495, 689
AcyI GRCGYC 1 cut(s) 381
AfiI CCNNNNNNNGG 3 cut(s) 198, 352, 462
AflIII ACRYGT 1 cut(s) 729
AgsI TTSAA 4 cut(s) 474, 487, 538, 736
AjnI CCWGG 1 cut(s) 529
AluBI AGCT 5 cut(s) 52, 301, 652, 706, 717
AluI AGCT 5 cut(s) 52, 301, 652, 706, 717
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 2 cut(s) 137, 657
AlwI GGATC 2 cut(s) 286, 630
Ama87I CYCGRG 2 cut(s) 350, 427
AoxI GGCC 6 cut(s) 204, 208, 244, 316, 465, 643
ApaI GGGCCC 1 cut(s) 469
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 2 cut(s) 495, 689
AspLEI GCGC 3 cut(s) 26, 165, 411
AspS9I GGNCC 3 cut(s) 355, 465, 466
AsuC2I CCSGG 2 cut(s) 107, 219
AsuHPI GGTGA 1 cut(s) 33
AvaI CYCGRG 2 cut(s) 350, 427
AvaII GGWCC 1 cut(s) 355
BaeGI GKGCMC 1 cut(s) 469
BalI TGGCCA 1 cut(s) 645
BanII GRGCYC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 1 cut(s) 89
BccI CCATC 2 cut(s) 155, 773
BceAI ACGGC 3 cut(s) 140, 259, 268
BcgI CGANNNNNNTGC 6 cut(s) 166, 200, 211, 245, 353, 387
BciT130I CCWGG 1 cut(s) 531
BcnI CCSGG 2 cut(s) 107, 219
BcoDI GTCTC 2 cut(s) 137, 657
BfaI CTAG 1 cut(s) 653
BfmI CTRYAG 1 cut(s) 763
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
BlsI GCNGC 9 cut(s) 18, 59, 76, 79, 82, 190, 212, 257, 320
Bme1390I CCNGG 3 cut(s) 107, 219, 531
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 2 cut(s) 350, 427
BmgT120I GGNCC 3 cut(s) 355, 465, 466
BmiI GGNNCC 2 cut(s) 467, 468
BmrFI CCNGG 3 cut(s) 107, 219, 531
BmsI GCATC 4 cut(s) 192, 231, 361, 427
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 2 cut(s) 32, 686
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 2 cut(s) 107, 219
BsaBI GATNNNNATC 1 cut(s) 621
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 2 cut(s) 137, 657
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 352, 462
Bse118I RCCGGY 1 cut(s) 206
Bse8I GATNNNNATC 1 cut(s) 621
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 4 cut(s) 147, 601, 643, 784
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 3 cut(s) 198, 352, 462
BseMII CTCAG 2 cut(s) 617, 727
BseRI GAGGAG 3 cut(s) 28, 293, 528
BseSI GKGCMC 1 cut(s) 469
BseX3I CGGCCG 2 cut(s) 208, 316
BseXI GCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 407
Bsh1236I CGCG 4 cut(s) 163, 213, 399, 409
Bsh1285I CGRYCG 2 cut(s) 211, 319
BshFI GGCC 6 cut(s) 206, 210, 246, 318, 467, 645
BsiEI CGRYCG 2 cut(s) 211, 319
BsiHKAI GWGCWC 1 cut(s) 434
BsiHKCI CYCGRG 2 cut(s) 350, 427
BsiSI CCGG 4 cut(s) 107, 174, 207, 219
BslFI GGGAC 2 cut(s) 143, 200
BslI CCNNNNNNNGG 3 cut(s) 198, 352, 462
BsmAI GTCTC 2 cut(s) 137, 657
BsmFI GGGAC 2 cut(s) 143, 200
BsnI GGCC 6 cut(s) 206, 210, 246, 318, 467, 645
Bso31I GGTCTC 2 cut(s) 137, 657
BsoBI CYCGRG 2 cut(s) 350, 427
Bsp120I GGGCCC 1 cut(s) 465
Bsp1286I GDGCHC 2 cut(s) 434, 469
Bsp143I GATC 3 cut(s) 291, 622, 750
BspANI GGCC 6 cut(s) 206, 210, 246, 318, 467, 645
BspCNI CTCAG 2 cut(s) 618, 726
BspFNI CGCG 4 cut(s) 163, 213, 399, 409
BspHI TCATGA 1 cut(s) 788
BspLI GGNNCC 2 cut(s) 467, 468
BspPI GGATC 2 cut(s) 286, 630
BspTNI GGTCTC 2 cut(s) 137, 657
BsrBI CCGCTC 4 cut(s) 83, 199, 321, 360
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 3 cut(s) 291, 622, 750
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 1 cut(s) 574
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 208, 248, 299
BstDEI CTNAG 2 cut(s) 626, 713
BstENI CCTNNNNNAGG 1 cut(s) 460
BstF5I GGATG 4 cut(s) 147, 601, 643, 784
BstFNI CGCG 4 cut(s) 163, 213, 399, 409
BstHHI GCGC 3 cut(s) 26, 165, 411
BstKTI GATC 3 cut(s) 294, 625, 753
BstMAI GTCTC 2 cut(s) 137, 657
BstMBI GATC 3 cut(s) 291, 622, 750
BstMCI CGRYCG 2 cut(s) 211, 319
BstMWI GCNNNNNNNGC 4 cut(s) 71, 80, 219, 252
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 3 cut(s) 105, 217, 529
BstSFI CTRYAG 1 cut(s) 763
BstSLI GKGCMC 1 cut(s) 469
BstUI CGCG 4 cut(s) 163, 213, 399, 409
BstV1I GCAGC 1 cut(s) 89
BstX2I RGATCY 2 cut(s) 291, 622
BstYI RGATCY 2 cut(s) 291, 622
BstZI CGGCCG 2 cut(s) 208, 316
BsuRI GGCC 6 cut(s) 206, 210, 246, 318, 467, 645
BtgZI GCGATG 1 cut(s) 169
BtsCI GGATG 4 cut(s) 147, 601, 643, 784
Cac8I GCNNGC 3 cut(s) 208, 248, 299
CciI TCATGA 1 cut(s) 788
CfoI GCGC 3 cut(s) 26, 165, 411
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 355, 465, 466
CseI GACGC 1 cut(s) 202
CviAII CATG 2 cut(s) 730, 789
DdeI CTNAG 2 cut(s) 626, 713
DpnI GATC 3 cut(s) 293, 624, 752
DpnII GATC 3 cut(s) 291, 622, 750
DraI TTTAAA 1 cut(s) 555
EaeI YGGCCR 4 cut(s) 208, 244, 316, 643
EagI CGGCCG 2 cut(s) 208, 316
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EciI GGCGGA 2 cut(s) 44, 61
EclXI CGGCCG 2 cut(s) 208, 316
Eco24I GRGCYC 1 cut(s) 469
Eco31I GGTCTC 2 cut(s) 137, 657
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 355
Eco52I CGGCCG 2 cut(s) 208, 316
Eco88I CYCGRG 2 cut(s) 350, 427
EcoNI CCTNNNNNAGG 1 cut(s) 460
EcoO109I RGGNCCY 2 cut(s) 465, 466
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 469
FaeI CATG 2 cut(s) 733, 792
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 2 cut(s) 143, 200
FatI CATG 2 cut(s) 729, 788
FauI CCCGC 2 cut(s) 168, 344
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
FokI GGATG 3 cut(s) 134, 608, 650
FriOI GRGCYC 1 cut(s) 469
FseI GGCCGGCC 1 cut(s) 210
Fsp4HI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
FspBI CTAG 1 cut(s) 653
GlaI GCGC 3 cut(s) 25, 164, 410
GluI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
GsuI CTGGAG 2 cut(s) 32, 686
HaeIII GGCC 6 cut(s) 206, 210, 246, 318, 467, 645
HapII CCGG 4 cut(s) 107, 174, 207, 219
HgaI GACGC 1 cut(s) 202
HhaI GCGC 3 cut(s) 26, 165, 411
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 2 cut(s) 733, 792
Hin6I GCGC 3 cut(s) 24, 163, 409
HinP1I GCGC 3 cut(s) 24, 163, 409
HincII GTYRAC 2 cut(s) 91, 727
HindII GTYRAC 2 cut(s) 91, 727
HinfI GANTC 5 cut(s) 98, 110, 267, 415, 783
HpaII CCGG 4 cut(s) 107, 174, 207, 219
HphI GGTGA 1 cut(s) 33
Hpy166II GTNNAC 2 cut(s) 91, 727
Hpy188I TCNGA 3 cut(s) 364, 512, 627
Hpy188III TCNNGA 3 cut(s) 565, 748, 789
Hpy8I GTNNAC 2 cut(s) 91, 727
Hpy99I CGWCG 6 cut(s) 92, 95, 98, 143, 383, 386
HpyAV CCTTC 5 cut(s) 124, 196, 466, 480, 719
HpyCH4III ACNGT 1 cut(s) 574
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 3 cut(s) 374, 424, 548
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 80, 219, 252
HpyF3I CTNAG 2 cut(s) 626, 713
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 2 cut(s) 733, 792
HspAI GCGC 3 cut(s) 24, 163, 409
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 3 cut(s) 291, 622, 750
LmnI GCTCC 5 cut(s) 57, 306, 365, 445, 711
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 4 cut(s) 192, 231, 361, 427
MaeI CTAG 1 cut(s) 653
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 451
MalI GATC 3 cut(s) 293, 624, 752
MbiI CCGCTC 4 cut(s) 83, 199, 321, 360
MboI GATC 3 cut(s) 291, 622, 750
MboII GAAGA 4 cut(s) 220, 319, 334, 550
MflI RGATCY 2 cut(s) 291, 622
MhlI GDGCHC 2 cut(s) 434, 469
MlsI TGGCCA 1 cut(s) 645
MluCI AATT 3 cut(s) 495, 600, 689
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 2 cut(s) 119, 261
MmeI TCCRAC 3 cut(s) 126, 387, 615
Mox20I TGGCCA 1 cut(s) 645
MroNI GCCGGC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 645
MseI TTAA 3 cut(s) 167, 447, 554
Msp20I TGGCCA 1 cut(s) 645
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 4 cut(s) 107, 174, 207, 219
MspR9I CCNGG 3 cut(s) 107, 219, 531
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 4 cut(s) 163, 213, 399, 409
MwoI GCNNNNNNNGC 4 cut(s) 71, 80, 219, 252
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 2 cut(s) 107, 219
NdeII GATC 3 cut(s) 291, 622, 750
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 2 cut(s) 733, 792
NlaIV GGNNCC 2 cut(s) 467, 468
NmeAIII GCCGAG 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 451
NspI RCATGY 1 cut(s) 733
PaeR7I CTCGAG 2 cut(s) 350, 427
PagI TCATGA 1 cut(s) 788
PciI ACATGT 1 cut(s) 729
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 375
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 3 cut(s) 98, 415, 783
PflFI GACNNNGTC 1 cut(s) 380
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 9 cut(s) 18, 59, 76, 79, 82, 190, 212, 257, 320
PleI GAGTC 2 cut(s) 118, 261
PpsI GAGTC 2 cut(s) 118, 261
PscI ACATGT 1 cut(s) 729
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 2 cut(s) 467, 468
PspOMI GGGCCC 1 cut(s) 465
PspPI GGNCC 3 cut(s) 355, 465, 466
PspXI VCTCGAGB 2 cut(s) 350, 427
PsuI RGATCY 2 cut(s) 291, 622
PsyI GACNNNGTC 1 cut(s) 380
RigI GGCCGGCC 1 cut(s) 210
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 3 cut(s) 167, 447, 554
SatI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
Sau3AI GATC 3 cut(s) 291, 622, 750
Sau96I GGNCC 3 cut(s) 355, 465, 466
SchI GAGTC 2 cut(s) 119, 261
ScrFI CCNGG 3 cut(s) 107, 219, 531
SduI GDGCHC 2 cut(s) 434, 469
SfaNI GCATC 4 cut(s) 192, 231, 361, 427
SfcI CTRYAG 1 cut(s) 763
Sfr274I CTCGAG 2 cut(s) 350, 427
SgrDI CGTCGACG 1 cut(s) 89
SinI GGWCC 1 cut(s) 355
SlaI CTCGAG 2 cut(s) 350, 427
SmiI ATTTAAAT 1 cut(s) 555
SmlI CTYRAG 3 cut(s) 350, 427, 668
SmoI CTYRAG 3 cut(s) 350, 427, 668
Sse9I AATT 3 cut(s) 495, 600, 689
SspMI CTAG 1 cut(s) 653
StyD4I CCNGG 3 cut(s) 105, 217, 529
SwaI ATTTAAAT 1 cut(s) 555
TaaI ACNGT 1 cut(s) 574
TaiI ACGT 1 cut(s) 384
TaqI TCGA 6 cut(s) 90, 231, 351, 369, 428, 492
TasI AATT 3 cut(s) 495, 600, 689
TauI GCSGC 8 cut(s) 19, 60, 77, 83, 191, 213, 258, 321
TfiI GAWTC 3 cut(s) 98, 415, 783
Tru1I TTAA 3 cut(s) 167, 447, 554
Tru9I TTAA 3 cut(s) 167, 447, 554
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 2 cut(s) 407, 448
TspGWI ACGGA 1 cut(s) 265
Tth111I GACNNNGTC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 355
XagI CCTNNNNNAGG 1 cut(s) 460
XapI RAATTY 2 cut(s) 495, 689
XceI RCATGY 1 cut(s) 733
XhoI CTCGAG 2 cut(s) 350, 427
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 1 cut(s) 653
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.