Rroxscaffold_5G00352830

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008450
Physical Location & Seq
Reverse (-)
30225316 .. 30227131
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352830.1

Sequence Viewer

Length: 645 bp
ATGTTCCTCCTCCAGCGGCGTTGCGCCTCCGCGCTCTCCGCACCCCTCCAGCTCCGCCGCTACGCCCAATCCGCCGCAGCCGCTCTCCTCGACGACGATTCCAACCCAGAGTCCTTTCCCCGACCCGACCCGAAATACGACGAGACCATCCTTGCCGTCCCGCGCTTAACCTCCGGCAAAAGCATCGCCGCCAAAGAGCGGAAGGCCGGCCGCGTCCCCGGCATCATCTTCGAGCAAGAGGACGGCCAGCACGGCGGCAATAAGCGACTCATCTCCGTCCGCACGAACCAGATCCGCAAGCTCCTCAATCATCTCGGCCGCTCTTTCTTCCTCTCCCGCCTCTTCCACCTCGAGGTCCGCTCCGACTTCGATGCAGACGACGTCGTTGAGAAAGTCCGCGTTTTACCGCGCTTGAAGATAGGTGCATATTTAAATACAATCAAGAGGACGGTAAAGTTCCTTTGCCCTGCGGATGTAATTCCTCCATATATTGATGTGGATCTGAGTGAGTTGGATGTTGGCCAAAAGATAGTTATGGGAGACCTCAAGGTTCATCCGACTCTCAAACTTATTCAGTCAAAAGATGAGCCAATTTGTAAGATCATGGGTTCAAGAGTTTCTGACCAACAAAAGAAAACTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

214

Amino Acids

24.06

Weight (kDa)

9.62

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L25p PF01386 52 - 121 6.6e-07 Ribosomal L25p family
Ribosomal_TL5_C PF14693 135 - 204 7.7e-15 Ribosomal protein TL5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
AbsI CCTCGAGG 1 cut(s) 350
AccBSI CCGCTC 4 cut(s) 83, 199, 321, 360
AccII CGCG 5 cut(s) 32, 163, 213, 399, 409
AclWI GGATC 2 cut(s) 286, 507
AcoI YGGCCR 4 cut(s) 208, 244, 316, 520
AcyI GRCGYC 1 cut(s) 381
AfiI CCNNNNNNNGG 2 cut(s) 198, 352
AgsI TTSAA 2 cut(s) 415, 612
AluBI AGCT 2 cut(s) 52, 301
AluI AGCT 2 cut(s) 52, 301
Alw26I GTCTC 2 cut(s) 137, 534
AlwI GGATC 2 cut(s) 286, 507
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 5 cut(s) 204, 208, 244, 316, 520
ApeKI GCWGC 1 cut(s) 77
AspLEI GCGC 4 cut(s) 26, 34, 165, 411
AspS9I GGNCC 1 cut(s) 355
AsuC2I CCSGG 1 cut(s) 219
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 1 cut(s) 355
BalI TGGCCA 1 cut(s) 522
BbvI GCAGC 1 cut(s) 89
BccI CCATC 1 cut(s) 155
BceAI ACGGC 3 cut(s) 140, 259, 268
BcgI CGANNNNNNTGC 6 cut(s) 166, 200, 211, 245, 353, 387
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 137, 534
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
BlsI GCNGC 9 cut(s) 18, 59, 76, 79, 82, 190, 212, 257, 320
Bme1390I CCNGG 1 cut(s) 219
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 219
BmsI GCATC 3 cut(s) 192, 231, 361
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 2 cut(s) 137, 534
BsaJI CCNNGG 1 cut(s) 217
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 352
Bse118I RCCGGY 1 cut(s) 206
Bse8I GATNNNNATC 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 4 cut(s) 147, 478, 520, 553
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 2 cut(s) 198, 352
BseMII CTCAG 1 cut(s) 494
BseRI GAGGAG 2 cut(s) 77, 293
BseX3I CGGCCG 2 cut(s) 208, 316
BseXI GCAGC 1 cut(s) 89
Bsh1236I CGCG 5 cut(s) 32, 163, 213, 399, 409
Bsh1285I CGRYCG 2 cut(s) 211, 319
BshFI GGCC 5 cut(s) 206, 210, 246, 318, 522
BsiEI CGRYCG 2 cut(s) 211, 319
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 3 cut(s) 174, 207, 219
BslFI GGGAC 2 cut(s) 143, 200
BslI CCNNNNNNNGG 2 cut(s) 198, 352
BsmAI GTCTC 2 cut(s) 137, 534
BsmFI GGGAC 2 cut(s) 143, 200
BsnI GGCC 5 cut(s) 206, 210, 246, 318, 522
Bso31I GGTCTC 2 cut(s) 137, 534
BsoBI CYCGRG 1 cut(s) 350
Bsp143I GATC 3 cut(s) 291, 499, 600
BspANI GGCC 5 cut(s) 206, 210, 246, 318, 522
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 5 cut(s) 32, 163, 213, 399, 409
BspPI GGATC 2 cut(s) 286, 507
BspTNI GGTCTC 2 cut(s) 137, 534
BsrBI CCGCTC 4 cut(s) 83, 199, 321, 360
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 3 cut(s) 291, 499, 600
BssNI GRCGYC 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 208, 248, 299
BstDEI CTNAG 1 cut(s) 503
BstF5I GGATG 4 cut(s) 147, 478, 520, 553
BstFNI CGCG 5 cut(s) 32, 163, 213, 399, 409
BstHHI GCGC 4 cut(s) 26, 34, 165, 411
BstKTI GATC 3 cut(s) 294, 502, 603
BstMAI GTCTC 2 cut(s) 137, 534
BstMBI GATC 3 cut(s) 291, 499, 600
BstMCI CGRYCG 2 cut(s) 211, 319
BstMWI GCNNNNNNNGC 5 cut(s) 38, 71, 80, 219, 252
BstSCI CCNGG 1 cut(s) 217
BstUI CGCG 5 cut(s) 32, 163, 213, 399, 409
BstV1I GCAGC 1 cut(s) 89
BstX2I RGATCY 2 cut(s) 291, 499
BstYI RGATCY 2 cut(s) 291, 499
BstZI CGGCCG 2 cut(s) 208, 316
BsuRI GGCC 5 cut(s) 206, 210, 246, 318, 522
BtgZI GCGATG 1 cut(s) 169
BtsCI GGATG 4 cut(s) 147, 478, 520, 553
Cac8I GCNNGC 3 cut(s) 208, 248, 299
CfoI GCGC 4 cut(s) 26, 34, 165, 411
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 355
CseI GACGC 1 cut(s) 202
CviAII CATG 1 cut(s) 604
CviJI RGCY 9 cut(s) 52, 80, 206, 210, 246, 301, 318, 522, 589
CviKI_1 RGCY 9 cut(s) 52, 80, 206, 210, 246, 301, 318, 522, 589
DdeI CTNAG 1 cut(s) 503
DpnI GATC 3 cut(s) 293, 501, 602
DpnII GATC 3 cut(s) 291, 499, 600
DraI TTTAAA 1 cut(s) 432
EaeI YGGCCR 4 cut(s) 208, 244, 316, 520
EagI CGGCCG 2 cut(s) 208, 316
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EciI GGCGGA 2 cut(s) 44, 61
EclXI CGGCCG 2 cut(s) 208, 316
Eco31I GGTCTC 2 cut(s) 137, 534
Eco47I GGWCC 1 cut(s) 355
Eco52I CGGCCG 2 cut(s) 208, 316
Eco88I CYCGRG 1 cut(s) 350
FaeI CATG 1 cut(s) 607
FaiI YATR 5 cut(s) 427, 487, 489, 536, 605
FaqI GGGAC 2 cut(s) 143, 200
FatI CATG 1 cut(s) 603
FauI CCCGC 2 cut(s) 168, 344
Fnu4HI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
FokI GGATG 4 cut(s) 134, 485, 527, 540
FseI GGCCGGCC 1 cut(s) 210
Fsp4HI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
GlaI GCGC 4 cut(s) 25, 33, 164, 410
GluI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
GsuI CTGGAG 1 cut(s) 32
HaeIII GGCC 5 cut(s) 206, 210, 246, 318, 522
HapII CCGG 3 cut(s) 174, 207, 219
HgaI GACGC 1 cut(s) 202
HhaI GCGC 4 cut(s) 26, 34, 165, 411
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 1 cut(s) 607
Hin6I GCGC 4 cut(s) 24, 32, 163, 409
HinP1I GCGC 4 cut(s) 24, 32, 163, 409
HinfI GANTC 4 cut(s) 98, 110, 267, 559
HpaII CCGG 3 cut(s) 174, 207, 219
Hpy188I TCNGA 4 cut(s) 364, 504, 558, 622
Hpy188III TCNNGA 2 cut(s) 442, 612
Hpy99I CGWCG 5 cut(s) 95, 98, 143, 383, 386
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 2 cut(s) 374, 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 71, 80, 219, 252
HpyF3I CTNAG 1 cut(s) 503
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 1 cut(s) 607
HspAI GCGC 4 cut(s) 24, 32, 163, 409
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 3 cut(s) 291, 499, 600
LmnI GCTCC 3 cut(s) 57, 306, 365
LpnPI CCDG 9 cut(s) 26, 62, 120, 187, 220, 232, 260, 302, 480
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 3 cut(s) 192, 231, 361
MaeII ACGT 1 cut(s) 381
MalI GATC 3 cut(s) 293, 501, 602
MbiI CCGCTC 4 cut(s) 83, 199, 321, 360
MboI GATC 3 cut(s) 291, 499, 600
MboII GAAGA 4 cut(s) 220, 319, 334, 427
MflI RGATCY 2 cut(s) 291, 499
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 2 cut(s) 477, 591
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 3 cut(s) 119, 261, 553
MmeI TCCRAC 4 cut(s) 126, 387, 492, 581
Mox20I TGGCCA 1 cut(s) 522
MroNI GCCGGC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 522
MseI TTAA 2 cut(s) 167, 431
Msp20I TGGCCA 1 cut(s) 522
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 3 cut(s) 174, 207, 219
MspR9I CCNGG 1 cut(s) 219
MvnI CGCG 5 cut(s) 32, 163, 213, 399, 409
MwoI GCNNNNNNNGC 5 cut(s) 38, 71, 80, 219, 252
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 219
NdeII GATC 3 cut(s) 291, 499, 600
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 1 cut(s) 607
NmeAIII GCCGAG 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 350
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 98
PflFI GACNNNGTC 1 cut(s) 380
PkrI GCNGC 9 cut(s) 18, 59, 76, 79, 82, 190, 212, 257, 320
PleI GAGTC 3 cut(s) 118, 261, 553
PpsI GAGTC 3 cut(s) 118, 261, 553
PspPI GGNCC 1 cut(s) 355
PspXI VCTCGAGB 1 cut(s) 350
PsuI RGATCY 2 cut(s) 291, 499
PsyI GACNNNGTC 1 cut(s) 380
RigI GGCCGGCC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 167, 431
SatI GCNGC 9 cut(s) 17, 58, 75, 78, 81, 189, 211, 256, 319
Sau3AI GATC 3 cut(s) 291, 499, 600
Sau96I GGNCC 1 cut(s) 355
SchI GAGTC 3 cut(s) 119, 261, 553
ScrFI CCNGG 1 cut(s) 219
SetI ASST 9 cut(s) 54, 173, 303, 351, 357, 384, 424, 546, 552
SfaNI GCATC 3 cut(s) 192, 231, 361
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 1 cut(s) 355
SlaI CTCGAG 1 cut(s) 350
SmiI ATTTAAAT 1 cut(s) 432
SmlI CTYRAG 2 cut(s) 350, 545
SmoI CTYRAG 2 cut(s) 350, 545
Sse9I AATT 2 cut(s) 477, 591
StyD4I CCNGG 1 cut(s) 217
SwaI ATTTAAAT 1 cut(s) 432
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 1 cut(s) 384
TaqI TCGA 4 cut(s) 90, 231, 351, 369
TasI AATT 2 cut(s) 477, 591
TauI GCSGC 8 cut(s) 19, 60, 77, 83, 191, 213, 258, 321
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 2 cut(s) 167, 431
Tru9I TTAA 2 cut(s) 167, 431
TseI GCWGC 1 cut(s) 77
TspDTI ATGAA 1 cut(s) 542
TspGWI ACGGA 1 cut(s) 265
Tth111I GACNNNGTC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 355
XhoI CTCGAG 1 cut(s) 350
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.