Rh4CG157100

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008450
Physical Location & Seq
Reverse (-)
34511648 .. 34516106
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG157100.1

Sequence Viewer

Length: 576 bp
ATGTTCCTCCTCCAGCGGCGTTGCGCCTCCGCGCTCTCCGCACCCCTCCAGCTCCGCCGCTACACCCAATCCGCCGCAGCTGCTCTCGTCGACGACGATTCCAACCCGGAGTCCTTCCCCCGACCCGAACCGAAATACGACGAGACCATCCTTGCCGTCCCGCGCTTAACCTCCGGCAAAAGCATCGCCGCCAAAGAGCGGAAGGCCGGCCCCGTCCTCGGCATCATCTTCGAGCAAGAGGACGGCCAGCACGGCGGCAATAAACGACTCATCTCCGTCCGCACGAACCAGATCCGCAAGCTCCTCAATCATCTCGGCCGCTCTTTCTTCCTCTCCCGCCTCTTCCACCTCGAGGTCCGCTCCGACTTCGATGCAGACGACGTCGTTGAGAAAGTCCGCGTTCTACCGCGCTTGATTCATCTGCACTCGAGCACCGATGCTCCGCTTAATGTGACCTTCATAAGGGCCCCTTCAAATGCTTTGTTGAAAGTCGAAATTCCTCTTGTATTCAGAGGAGATGATATCTCTCCTGGATTGAAGAAAGTTTTTGCCAGAACCGAGGACAAACATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

191

Amino Acids

21.36

Weight (kDa)

9.66

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L25p PF01386 52 - 153 4.9e-07 Ribosomal L25p family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
AbsI CCTCGAGG 1 cut(s) 350
AccBSI CCGCTC 3 cut(s) 199, 321, 360
AccI GTMKAC 1 cut(s) 90
AccII CGCG 4 cut(s) 32, 163, 399, 409
AclWI GGATC 1 cut(s) 286
AcoI YGGCCR 2 cut(s) 244, 316
AcsI RAATTY 1 cut(s) 495
AcyI GRCGYC 1 cut(s) 381
AfiI CCNNNNNNNGG 4 cut(s) 198, 218, 352, 462
AgsI TTSAA 3 cut(s) 474, 487, 538
AjnI CCWGG 1 cut(s) 529
AluBI AGCT 3 cut(s) 52, 80, 301
AluI AGCT 3 cut(s) 52, 80, 301
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 1 cut(s) 286
Ama87I CYCGRG 2 cut(s) 350, 427
AoxI GGCC 5 cut(s) 204, 208, 244, 316, 465
ApaI GGGCCC 1 cut(s) 469
ApeKI GCWGC 2 cut(s) 77, 80
ApoI RAATTY 1 cut(s) 495
AspLEI GCGC 4 cut(s) 26, 34, 165, 411
AspS9I GGNCC 4 cut(s) 209, 355, 465, 466
AsuC2I CCSGG 1 cut(s) 107
AvaI CYCGRG 2 cut(s) 350, 427
AvaII GGWCC 1 cut(s) 355
BaeGI GKGCMC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 2 cut(s) 67, 89
BccI CCATC 1 cut(s) 155
BceAI ACGGC 3 cut(s) 140, 259, 268
BcgI CGANNNNNNTGC 6 cut(s) 166, 200, 211, 245, 353, 387
BciT130I CCWGG 1 cut(s) 531
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
BlsI GCNGC 8 cut(s) 18, 59, 76, 79, 82, 190, 257, 320
Bme1390I CCNGG 2 cut(s) 107, 531
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 2 cut(s) 350, 427
BmgT120I GGNCC 4 cut(s) 209, 355, 465, 466
BmiI GGNNCC 3 cut(s) 211, 467, 468
BmrFI CCNGG 2 cut(s) 107, 531
BmsI GCATC 4 cut(s) 192, 231, 361, 427
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 217, 558
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 4 cut(s) 198, 218, 352, 462
Bse118I RCCGGY 1 cut(s) 206
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 217, 558
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 4 cut(s) 198, 218, 352, 462
BseRI GAGGAG 2 cut(s) 293, 528
BseSI GKGCMC 1 cut(s) 469
BseX3I CGGCCG 1 cut(s) 316
BseXI GCAGC 2 cut(s) 67, 89
BsgI GTGCAG 1 cut(s) 407
Bsh1236I CGCG 4 cut(s) 32, 163, 399, 409
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 5 cut(s) 206, 210, 246, 318, 467
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 434
BsiHKCI CYCGRG 2 cut(s) 350, 427
BsiSI CCGG 3 cut(s) 107, 174, 207
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 4 cut(s) 198, 218, 352, 462
BsmAI GTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 5 cut(s) 206, 210, 246, 318, 467
Bso31I GGTCTC 1 cut(s) 137
BsoBI CYCGRG 2 cut(s) 350, 427
Bsp120I GGGCCC 1 cut(s) 465
Bsp1286I GDGCHC 2 cut(s) 434, 469
Bsp143I GATC 1 cut(s) 291
BspANI GGCC 5 cut(s) 206, 210, 246, 318, 467
BspFNI CGCG 4 cut(s) 32, 163, 399, 409
BspLI GGNNCC 3 cut(s) 211, 467, 468
BspPI GGATC 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 137
BsrBI CCGCTC 3 cut(s) 199, 321, 360
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 217, 558
BssMI GATC 1 cut(s) 291
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 531
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 208, 248, 299
BstENI CCTNNNNNAGG 1 cut(s) 460
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 4 cut(s) 32, 163, 399, 409
BstHHI GCGC 4 cut(s) 26, 34, 165, 411
BstKTI GATC 1 cut(s) 294
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 1 cut(s) 291
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 3 cut(s) 38, 80, 252
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 2 cut(s) 105, 529
BstSLI GKGCMC 1 cut(s) 469
BstUI CGCG 4 cut(s) 32, 163, 399, 409
BstV1I GCAGC 2 cut(s) 67, 89
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BstZI CGGCCG 1 cut(s) 316
BsuRI GGCC 5 cut(s) 206, 210, 246, 318, 467
BtgZI GCGATG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 147
Cac8I GCNNGC 3 cut(s) 208, 248, 299
CfoI GCGC 4 cut(s) 26, 34, 165, 411
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 4 cut(s) 209, 355, 465, 466
CviAII CATG 1 cut(s) 569
CviJI RGCY 8 cut(s) 52, 80, 206, 210, 246, 301, 318, 467
CviKI_1 RGCY 8 cut(s) 52, 80, 206, 210, 246, 301, 318, 467
DpnI GATC 1 cut(s) 293
DpnII GATC 1 cut(s) 291
EaeI YGGCCR 2 cut(s) 244, 316
EagI CGGCCG 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EciI GGCGGA 2 cut(s) 44, 61
EclXI CGGCCG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 469
Eco31I GGTCTC 1 cut(s) 137
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 355
Eco52I CGGCCG 1 cut(s) 316
Eco88I CYCGRG 2 cut(s) 350, 427
EcoNI CCTNNNNNAGG 1 cut(s) 460
EcoO109I RGGNCCY 2 cut(s) 465, 466
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 469
FaeI CATG 1 cut(s) 572
FaiI YATR 2 cut(s) 461, 570
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 1 cut(s) 143
FatI CATG 1 cut(s) 568
FauI CCCGC 2 cut(s) 168, 344
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 469
FseI GGCCGGCC 1 cut(s) 210
Fsp4HI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
GlaI GCGC 4 cut(s) 25, 33, 164, 410
GluI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
GsuI CTGGAG 1 cut(s) 32
HaeIII GGCC 5 cut(s) 206, 210, 246, 318, 467
HapII CCGG 3 cut(s) 107, 174, 207
HhaI GCGC 4 cut(s) 26, 34, 165, 411
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 1 cut(s) 572
Hin6I GCGC 4 cut(s) 24, 32, 163, 409
HinP1I GCGC 4 cut(s) 24, 32, 163, 409
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 4 cut(s) 98, 110, 267, 415
HpaII CCGG 3 cut(s) 107, 174, 207
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 364, 512
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 6 cut(s) 92, 95, 98, 143, 383, 386
HpyAV CCTTC 4 cut(s) 124, 196, 466, 480
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 2 cut(s) 374, 424
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 80, 252
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 1 cut(s) 572
HspAI GCGC 4 cut(s) 24, 32, 163, 409
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 1 cut(s) 291
LmnI GCTCC 4 cut(s) 57, 306, 365, 445
Lsp1109I GCAGC 2 cut(s) 67, 89
LweI GCATC 4 cut(s) 192, 231, 361, 427
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 451
MalI GATC 1 cut(s) 293
MbiI CCGCTC 3 cut(s) 199, 321, 360
MboI GATC 1 cut(s) 291
MboII GAAGA 4 cut(s) 220, 319, 334, 550
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 2 cut(s) 434, 469
MluCI AATT 1 cut(s) 495
MlyI GAGTC 2 cut(s) 119, 261
MmeI TCCRAC 2 cut(s) 126, 387
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 2 cut(s) 167, 447
MspA1I CMGCKG 2 cut(s) 16, 80
MspI CCGG 3 cut(s) 107, 174, 207
MspR9I CCNGG 2 cut(s) 107, 531
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 4 cut(s) 32, 163, 399, 409
MwoI GCNNNNNNNGC 3 cut(s) 38, 80, 252
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 107
NdeII GATC 1 cut(s) 291
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 1 cut(s) 572
NlaIV GGNNCC 3 cut(s) 211, 467, 468
NmeAIII GCCGAG 2 cut(s) 198, 294
NmuCI GTSAC 1 cut(s) 451
PaeR7I CTCGAG 2 cut(s) 350, 427
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 375
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 98, 415
PflFI GACNNNGTC 1 cut(s) 380
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 8 cut(s) 18, 59, 76, 79, 82, 190, 257, 320
PleI GAGTC 2 cut(s) 118, 261
PpsI GAGTC 2 cut(s) 118, 261
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 3 cut(s) 211, 467, 468
PspOMI GGGCCC 1 cut(s) 465
PspPI GGNCC 4 cut(s) 209, 355, 465, 466
PspXI VCTCGAGB 2 cut(s) 350, 427
PsuI RGATCY 1 cut(s) 291
PsyI GACNNNGTC 1 cut(s) 380
PvuII CAGCTG 1 cut(s) 80
RigI GGCCGGCC 1 cut(s) 210
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 167, 447
SatI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
Sau3AI GATC 1 cut(s) 291
Sau96I GGNCC 4 cut(s) 209, 355, 465, 466
SchI GAGTC 2 cut(s) 119, 261
ScrFI CCNGG 2 cut(s) 107, 531
SduI GDGCHC 2 cut(s) 434, 469
SetI ASST 8 cut(s) 54, 82, 173, 303, 351, 357, 384, 458
SfaNI GCATC 4 cut(s) 192, 231, 361, 427
Sfr274I CTCGAG 2 cut(s) 350, 427
SgrDI CGTCGACG 1 cut(s) 89
SinI GGWCC 1 cut(s) 355
SlaI CTCGAG 2 cut(s) 350, 427
SmlI CTYRAG 2 cut(s) 350, 427
SmoI CTYRAG 2 cut(s) 350, 427
Sse9I AATT 1 cut(s) 495
StyD4I CCNGG 2 cut(s) 105, 529
TaiI ACGT 1 cut(s) 384
TaqI TCGA 6 cut(s) 90, 231, 351, 369, 428, 492
TasI AATT 1 cut(s) 495
TauI GCSGC 6 cut(s) 19, 60, 77, 191, 258, 321
TfiI GAWTC 2 cut(s) 98, 415
Tru1I TTAA 2 cut(s) 167, 447
Tru9I TTAA 2 cut(s) 167, 447
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 77, 80
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 2 cut(s) 407, 448
TspGWI ACGGA 1 cut(s) 265
Tth111I GACNNNGTC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 355
XagI CCTNNNNNAGG 1 cut(s) 460
XapI RAATTY 1 cut(s) 495
XhoI CTCGAG 2 cut(s) 350, 427
XmiI GTMKAC 1 cut(s) 90
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.