RchiOBHm_Chr4g0408291

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008450
Physical Location & Seq
Reverse (-)
30972854 .. 30973654
801 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37945

Sequence Viewer

Length: 666 bp
ATGTTCCTCCTCCAGCGGCGTTGCGCCTCCGCGCTCTCCGCACCCTTCCAGCTCCGCCGCTACACCCAATCCGCCGCAGCCGCTCTCGTCGACGACGATTCCAACCCGGAGTCCTTCCCCCGACCCGAACTGAAATACGACGAGACCATCCTTGCCGTCCCGCGCTTAACCTCCTGCAAAAGCATCGCCGCCAAAGAGCAGAAGGCCGGCCCCGTCCCCGACATCATCTTCGAGCAAGAGGACGGCCAGCACGGCGGCAATAAACGACTCATCTCCTTCCGCACGAACCAGATCCGCAAGCTCCTCAATCATCTCGGCCGCTCTTTCTTCCTCTCTCGCCTCTTCCACCTCGAGGTCCGCTCCGACTTCGATGCAGACGACGTCGTTGAGAAAGTCCGCGTTTTACCGCGCTTGATTCATCTACACTCGAGCACCGATGCTCCGCTTAATGTGACCTTCATAAGGGCCCCTTCAAATGCTTTGTTGAAAGTCGAAATTCCTCTTGTATTCAGAGGAGATGATATCTCTCCTGGATTGAAGAAAGGTAACAACAACCTTCAAAATTTCGCAATCTATCTCAAGAATTTGTGTTCAGTTCAGTGGGAGTTTAAGATTATAGCTAAGAAGGTTATAGAATTTCCAGATCGATTAGAGGATAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

221

Amino Acids

25.13

Weight (kDa)

9.28

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
AbsI CCTCGAGG 1 cut(s) 350
AccBSI CCGCTC 3 cut(s) 83, 321, 360
AccI GTMKAC 1 cut(s) 90
AccII CGCG 4 cut(s) 32, 163, 399, 409
AclWI GGATC 1 cut(s) 286
AcoI YGGCCR 2 cut(s) 244, 316
AcsI RAATTY 4 cut(s) 495, 562, 583, 635
AcyI GRCGYC 1 cut(s) 381
AfiI CCNNNNNNNGG 2 cut(s) 352, 462
AgsI TTSAA 4 cut(s) 474, 487, 538, 560
AjnI CCWGG 1 cut(s) 529
AluBI AGCT 3 cut(s) 52, 301, 620
AluI AGCT 3 cut(s) 52, 301, 620
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 1 cut(s) 286
Ama87I CYCGRG 2 cut(s) 350, 427
AoxI GGCC 5 cut(s) 204, 208, 244, 316, 465
ApaI GGGCCC 1 cut(s) 469
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 4 cut(s) 495, 562, 583, 635
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AspLEI GCGC 4 cut(s) 26, 34, 165, 411
AspS9I GGNCC 4 cut(s) 209, 355, 465, 466
AsuC2I CCSGG 1 cut(s) 107
AvaI CYCGRG 2 cut(s) 350, 427
AvaII GGWCC 1 cut(s) 355
BaeGI GKGCMC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 1 cut(s) 89
BccI CCATC 1 cut(s) 155
BceAI ACGGC 3 cut(s) 140, 259, 268
BcgI CGANNNNNNTGC 4 cut(s) 166, 200, 353, 387
BciT130I CCWGG 1 cut(s) 531
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
BlsI GCNGC 8 cut(s) 18, 59, 76, 79, 82, 190, 257, 320
Bme1390I CCNGG 2 cut(s) 107, 531
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 2 cut(s) 350, 427
BmgT120I GGNCC 4 cut(s) 209, 355, 465, 466
BmiI GGNNCC 3 cut(s) 211, 467, 468
BmrFI CCNGG 2 cut(s) 107, 531
BmsI GCATC 3 cut(s) 192, 361, 427
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 107
Bsa29I ATCGAT 1 cut(s) 646
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 137
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 462
Bse118I RCCGGY 1 cut(s) 206
BseBI CCWGG 1 cut(s) 531
BseCI ATCGAT 1 cut(s) 646
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 2 cut(s) 352, 462
BseRI GAGGAG 2 cut(s) 293, 528
BseSI GKGCMC 1 cut(s) 469
BseX3I CGGCCG 1 cut(s) 316
BseXI GCAGC 1 cut(s) 89
Bsh1236I CGCG 4 cut(s) 32, 163, 399, 409
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 5 cut(s) 206, 210, 246, 318, 467
BshVI ATCGAT 1 cut(s) 646
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 434
BsiHKCI CYCGRG 2 cut(s) 350, 427
BsiSI CCGG 2 cut(s) 107, 207
BslFI GGGAC 2 cut(s) 143, 200
BslI CCNNNNNNNGG 2 cut(s) 352, 462
BsmAI GTCTC 1 cut(s) 137
BsmFI GGGAC 2 cut(s) 143, 200
BsnI GGCC 5 cut(s) 206, 210, 246, 318, 467
Bso31I GGTCTC 1 cut(s) 137
BsoBI CYCGRG 2 cut(s) 350, 427
Bsp120I GGGCCC 1 cut(s) 465
Bsp1286I GDGCHC 2 cut(s) 434, 469
Bsp143I GATC 2 cut(s) 291, 643
BspANI GGCC 5 cut(s) 206, 210, 246, 318, 467
BspDI ATCGAT 1 cut(s) 646
BspFNI CGCG 4 cut(s) 32, 163, 399, 409
BspLI GGNNCC 3 cut(s) 211, 467, 468
BspPI GGATC 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 137
BsrBI CCGCTC 3 cut(s) 83, 321, 360
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssMI GATC 2 cut(s) 291, 643
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 531
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 208, 248, 299
BstDEI CTNAG 1 cut(s) 621
BstENI CCTNNNNNAGG 1 cut(s) 460
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 4 cut(s) 32, 163, 399, 409
BstHHI GCGC 4 cut(s) 26, 34, 165, 411
BstKTI GATC 2 cut(s) 294, 646
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 2 cut(s) 291, 643
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 3 cut(s) 38, 80, 252
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 2 cut(s) 105, 529
BstSLI GKGCMC 1 cut(s) 469
BstUI CGCG 4 cut(s) 32, 163, 399, 409
BstV1I GCAGC 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BstZI CGGCCG 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 646
BsuRI GGCC 5 cut(s) 206, 210, 246, 318, 467
BsuTUI ATCGAT 1 cut(s) 646
BtgZI GCGATG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 605
Cac8I GCNNGC 3 cut(s) 208, 248, 299
CfoI GCGC 4 cut(s) 26, 34, 165, 411
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 4 cut(s) 209, 355, 465, 466
ClaI ATCGAT 1 cut(s) 646
CviJI RGCY 9 cut(s) 52, 80, 206, 210, 246, 301, 318, 467, 620
CviKI_1 RGCY 9 cut(s) 52, 80, 206, 210, 246, 301, 318, 467, 620
DdeI CTNAG 1 cut(s) 621
DpnI GATC 2 cut(s) 293, 645
DpnII GATC 2 cut(s) 291, 643
EaeI YGGCCR 2 cut(s) 244, 316
EagI CGGCCG 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EciI GGCGGA 2 cut(s) 44, 61
EclXI CGGCCG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 469
Eco31I GGTCTC 1 cut(s) 137
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 355
Eco52I CGGCCG 1 cut(s) 316
Eco88I CYCGRG 2 cut(s) 350, 427
EcoNI CCTNNNNNAGG 1 cut(s) 460
EcoO109I RGGNCCY 2 cut(s) 465, 466
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 469
FaiI YATR 3 cut(s) 461, 617, 632
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 2 cut(s) 143, 200
FauI CCCGC 1 cut(s) 168
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 469
FseI GGCCGGCC 1 cut(s) 210
Fsp4HI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
GlaI GCGC 4 cut(s) 25, 33, 164, 410
GluI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
HaeIII GGCC 5 cut(s) 206, 210, 246, 318, 467
HapII CCGG 2 cut(s) 107, 207
HhaI GCGC 4 cut(s) 26, 34, 165, 411
Hin1I GRCGYC 1 cut(s) 381
Hin6I GCGC 4 cut(s) 24, 32, 163, 409
HinP1I GCGC 4 cut(s) 24, 32, 163, 409
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 4 cut(s) 98, 110, 267, 415
HpaII CCGG 2 cut(s) 107, 207
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 364, 512
Hpy188III TCNNGA 2 cut(s) 580, 641
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 6 cut(s) 92, 95, 98, 143, 383, 386
HpyAV CCTTC 8 cut(s) 55, 124, 196, 286, 466, 480, 566, 619
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 2 cut(s) 177, 374
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 80, 252
HpyF3I CTNAG 1 cut(s) 621
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 381
HspAI GCGC 4 cut(s) 24, 32, 163, 409
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 2 cut(s) 291, 643
LmnI GCTCC 4 cut(s) 57, 306, 365, 445
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 3 cut(s) 192, 361, 427
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 2 cut(s) 451, 545
MalI GATC 2 cut(s) 293, 645
MbiI CCGCTC 3 cut(s) 83, 321, 360
MboI GATC 2 cut(s) 291, 643
MboII GAAGA 4 cut(s) 220, 319, 334, 550
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 2 cut(s) 434, 469
MluCI AATT 4 cut(s) 495, 562, 583, 635
MlyI GAGTC 2 cut(s) 119, 261
MmeI TCCRAC 2 cut(s) 126, 387
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 3 cut(s) 167, 447, 609
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 2 cut(s) 107, 207
MspR9I CCNGG 2 cut(s) 107, 531
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 4 cut(s) 32, 163, 399, 409
MwoI GCNNNNNNNGC 3 cut(s) 38, 80, 252
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 107
NdeII GATC 2 cut(s) 291, 643
NgoMIV GCCGGC 1 cut(s) 206
NlaIV GGNNCC 3 cut(s) 211, 467, 468
NmeAIII GCCGAG 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 451
PaeR7I CTCGAG 2 cut(s) 350, 427
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 375
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 98, 415
PflFI GACNNNGTC 1 cut(s) 380
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 8 cut(s) 18, 59, 76, 79, 82, 190, 257, 320
PleI GAGTC 2 cut(s) 118, 261
PpsI GAGTC 2 cut(s) 118, 261
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 3 cut(s) 211, 467, 468
PspOMI GGGCCC 1 cut(s) 465
PspPI GGNCC 4 cut(s) 209, 355, 465, 466
PspXI VCTCGAGB 2 cut(s) 350, 427
PsuI RGATCY 1 cut(s) 291
PsyI GACNNNGTC 1 cut(s) 380
RigI GGCCGGCC 1 cut(s) 210
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 3 cut(s) 167, 447, 609
SatI GCNGC 8 cut(s) 17, 58, 75, 78, 81, 189, 256, 319
Sau3AI GATC 2 cut(s) 291, 643
Sau96I GGNCC 4 cut(s) 209, 355, 465, 466
SchI GAGTC 2 cut(s) 119, 261
ScrFI CCNGG 2 cut(s) 107, 531
SduI GDGCHC 2 cut(s) 434, 469
SfaNI GCATC 3 cut(s) 192, 361, 427
Sfr274I CTCGAG 2 cut(s) 350, 427
SgrDI CGTCGACG 1 cut(s) 89
SinI GGWCC 1 cut(s) 355
SlaI CTCGAG 2 cut(s) 350, 427
SmlI CTYRAG 3 cut(s) 350, 427, 578
SmoI CTYRAG 3 cut(s) 350, 427, 578
Sse9I AATT 4 cut(s) 495, 562, 583, 635
StyD4I CCNGG 2 cut(s) 105, 529
TaiI ACGT 1 cut(s) 384
TaqI TCGA 7 cut(s) 90, 231, 351, 369, 428, 492, 646
TasI AATT 4 cut(s) 495, 562, 583, 635
TauI GCSGC 7 cut(s) 19, 60, 77, 83, 191, 258, 321
TfiI GAWTC 2 cut(s) 98, 415
Tru1I TTAA 3 cut(s) 167, 447, 609
Tru9I TTAA 3 cut(s) 167, 447, 609
TscAI CASTG 1 cut(s) 605
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 2 cut(s) 407, 448
TspRI CASTG 1 cut(s) 605
Tth111I GACNNNGTC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 355
XagI CCTNNNNNAGG 1 cut(s) 460
XapI RAATTY 4 cut(s) 495, 562, 583, 635
XhoI CTCGAG 2 cut(s) 350, 427
XmiI GTMKAC 1 cut(s) 90
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.