pycom12g05200

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001598
Physical Location & Seq
Reverse (-)
5057343 .. 5060419
3077 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1008 bp
ATGTATACAGACATGGAGGCCCAAATTCATCACCTTGAGCAAGAAGCGTACTGTGCGGTCCTACGGGCATTTAAAGCGCAGTCAAATGCAATTACTTGGGAGAAGGAAGGTTTGATTACTGAACTTAGGAAAGAACTGAGAGTATCAGATGATGAACACAGAGAGCTTCTGACCAGGGTTAATTCTGAGGACATCATCGATAAGATAAGAGTGTGGAGAGACAAAGGTGGTCATCAAGCTGCCAGGCTTACTTCATCTCAGAATGCATATGAACTTTTACCTAGTCCTACTATTTCAGCGTCCCGAAAGAAACAGAAAACATCACAATCGCATGGTCAGCCATTCCCTGGTTTATCCTCAATGAAGTCCATGAAATACCCTTCAACAGGACCTGCTGGAAGTAGACAATTCACCCGCAGTTCTTCTGGGCCCTTTGTCGAAGCGGCTGTTGCAGAAGCATCTGATTCATTAGTTGGAAGGAAAGTGTGGACAAGGTGGCCTGAAGACAACAGCTTCTATGAAGCCGTTATCACTTACTACAACCCAGCTGAGGGTCGACATGCTTTGGTCTATGACATACATACACCAAATGAGACATGGGAATGGGTTGATCTCAAAGAGATCTCTCCGGAGGATATCTGTTGGGAAGATCCTGGCCCATCAGGACGCAGGGCTAAGAAATCTACTAGCCAGACCACAAGAAGGCGAGGACCCACAAAGTCTCAATCAAAGAAAGAACTTCCATCACAAAATGGTATAGGGAAGGTGCCAGATGATTTGGAATTATTAAATACTGATACCTTGGTAAAGGAGGTGGAGAGAGTTTTCAATGCAAGTGAGCCAGATCCTTTTGAGCTCGAAAAAGCAAGGAAAATGCTGAAAGATCACGAACAGGCCCTGGTTGACGCCATTCAAAGGCTGGCATATGCATCGGATGGTGAAAGTGGTAATAGATATATGTCAGATGGAGAGCACACATTTCTGCACAGTCGGTCAATGGATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

336

Amino Acids

37.91

Weight (kDa)

5.99

Isoelectric Point (pI)

51.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 7 - 75 3.8e-28 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 766
AccB7I CCANNNNNTGG 1 cut(s) 347
AccI GTMKAC 3 cut(s) 5, 403, 556
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 3 cut(s) 56, 415, 443
AclWI GGATC 2 cut(s) 644, 839
AcsI RAATTY 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 522
AcyI GRCGYC 1 cut(s) 906
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 6 cut(s) 347, 386, 550, 551, 702, 915
AgsI TTSAA 3 cut(s) 384, 829, 914
AjnI CCWGG 5 cut(s) 173, 242, 346, 652, 897
AluBI AGCT 5 cut(s) 166, 239, 513, 548, 856
AluI AGCT 5 cut(s) 166, 239, 513, 548, 856
Alw21I GWGCWC 2 cut(s) 858, 975
Alw26I GTCTC 3 cut(s) 213, 587, 726
AlwI GGATC 2 cut(s) 644, 839
AlwNI CAGNNNCTG 2 cut(s) 392, 898
Aor13HI TCCGGA 1 cut(s) 628
AoxI GGCC 5 cut(s) 18, 428, 497, 655, 894
ApaI GGGCCC 1 cut(s) 432
ApeKI GCWGC 1 cut(s) 239
ApoI RAATTY 1 cut(s) 24
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 8 cut(s) 19, 58, 389, 428, 429, 656, 710, 895
AsuHPI GGTGA 3 cut(s) 23, 403, 950
AvaII GGWCC 3 cut(s) 58, 389, 710
BaeGI GKGCMC 1 cut(s) 432
BaeI ACNNNNGTAYC 2 cut(s) 789, 822
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 2 cut(s) 432, 858
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 2 cut(s) 858, 975
BbvCI CCTCAGC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 226
BccI CCATC 5 cut(s) 667, 751, 929, 959, 995
BceAI ACGGC 1 cut(s) 509
BcgI CGANNNNNNTGC 2 cut(s) 912, 946
BciT130I CCWGG 5 cut(s) 175, 244, 348, 654, 899
BcoDI GTCTC 3 cut(s) 213, 587, 726
BfaI CTAG 2 cut(s) 282, 687
BfuAI ACCTGC 1 cut(s) 400
BglII AGATCT 1 cut(s) 621
BisI GCNGC 2 cut(s) 240, 444
BlsI GCNGC 2 cut(s) 241, 445
Bme1390I CCNGG 5 cut(s) 175, 244, 348, 654, 899
Bme18I GGWCC 3 cut(s) 58, 389, 710
BmgT120I GGNCC 8 cut(s) 19, 58, 389, 428, 429, 656, 710, 895
BmiI GGNNCC 3 cut(s) 430, 712, 768
BmrFI CCNGG 5 cut(s) 175, 244, 348, 654, 899
BmsI GCATC 2 cut(s) 467, 938
BpiI GAAGAC 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 56
Bsa29I ATCGAT 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 906
BsaJI CCNNGG 4 cut(s) 174, 346, 801, 897
BsaWI WCCGGW 1 cut(s) 628
Bsc4I CCNNNNNNNGG 6 cut(s) 347, 386, 550, 551, 702, 915
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 5 cut(s) 175, 244, 348, 654, 899
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 4 cut(s) 174, 346, 801, 897
BseGI GGATG 2 cut(s) 940, 1006
BseLI CCNNNNNNNGG 6 cut(s) 347, 386, 550, 551, 702, 915
BseMII CTCAG 4 cut(s) 128, 177, 272, 540
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 1 cut(s) 226
BseYI CCCAGC 1 cut(s) 544
BsgI GTGCAG 1 cut(s) 968
BshFI GGCC 5 cut(s) 20, 430, 499, 657, 896
BshNI GGYRCC 1 cut(s) 766
BshVI ATCGAT 1 cut(s) 198
BsiHKAI GWGCWC 2 cut(s) 858, 975
BsiSI CCGG 1 cut(s) 629
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 6 cut(s) 347, 386, 550, 551, 702, 915
BsmAI GTCTC 3 cut(s) 213, 587, 726
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 5 cut(s) 20, 430, 499, 657, 896
Bsp120I GGGCCC 1 cut(s) 428
Bsp1286I GDGCHC 3 cut(s) 432, 858, 975
Bsp13I TCCGGA 1 cut(s) 628
Bsp143I GATC 5 cut(s) 610, 621, 649, 844, 883
BspACI CCGC 3 cut(s) 56, 415, 443
BspANI GGCC 5 cut(s) 20, 430, 499, 657, 896
BspCNI CTCAG 4 cut(s) 129, 178, 271, 541
BspDI ATCGAT 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 628
BspLI GGNNCC 3 cut(s) 430, 712, 768
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 2 cut(s) 644, 839
BspT107I GGYRCC 1 cut(s) 766
BssECI CCNNGG 4 cut(s) 174, 346, 801, 897
BssMI GATC 5 cut(s) 610, 621, 649, 844, 883
BssNAI GTATAC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 906
BssT1I CCWWGG 1 cut(s) 801
Bst1107I GTATAC 1 cut(s) 6
Bst2UI CCWGG 5 cut(s) 175, 244, 348, 654, 899
Bst4CI ACNGT 2 cut(s) 53, 989
BstACI GRCGYC 1 cut(s) 906
BstC8I GCNNGC 1 cut(s) 921
BstDEI CTNAG 6 cut(s) 125, 137, 186, 258, 549, 675
BstENI CCTNNNNNAGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 940, 1006
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 5 cut(s) 613, 624, 652, 847, 886
BstMAI GTCTC 3 cut(s) 213, 587, 726
BstMBI GATC 5 cut(s) 610, 621, 649, 844, 883
BstMWI GCNNNNNNNGC 4 cut(s) 53, 74, 337, 449
BstNI CCWGG 5 cut(s) 175, 244, 348, 654, 899
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 5 cut(s) 173, 242, 346, 652, 897
BstSLI GKGCMC 1 cut(s) 432
BstV1I GCAGC 1 cut(s) 226
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 3 cut(s) 621, 649, 844
BstYI RGATCY 3 cut(s) 621, 649, 844
BstZ17I GTATAC 1 cut(s) 6
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 5 cut(s) 20, 430, 499, 657, 896
BsuTUI ATCGAT 1 cut(s) 198
BtsCI GGATG 2 cut(s) 940, 1006
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 1 cut(s) 921
CaiI CAGNNNCTG 2 cut(s) 392, 898
CfoI GCGC 1 cut(s) 79
Cfr13I GGNCC 8 cut(s) 19, 58, 389, 428, 429, 656, 710, 895
ClaI ATCGAT 1 cut(s) 198
CseI GACGC 3 cut(s) 288, 675, 914
Csp6I GTAC 1 cut(s) 49
CviAII CATG 5 cut(s) 13, 332, 370, 560, 597
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 6 cut(s) 125, 137, 186, 258, 549, 675
DpnI GATC 5 cut(s) 612, 623, 651, 846, 885
DpnII GATC 5 cut(s) 610, 621, 649, 844, 883
DraI TTTAAA 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 1 cut(s) 801
Eco24I GRGCYC 2 cut(s) 432, 858
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 3 cut(s) 58, 389, 710
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 1 cut(s) 522
EcoICRI GAGCTC 1 cut(s) 856
EcoNI CCTNNNNNAGG 1 cut(s) 384
EcoO109I RGGNCCY 4 cut(s) 389, 429, 710, 895
EcoRII CCWGG 5 cut(s) 173, 242, 346, 652, 897
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 801
EcoT22I ATGCAT 2 cut(s) 268, 931
EcoT38I GRGCYC 2 cut(s) 432, 858
ErhI CCWWGG 1 cut(s) 801
FaeI CATG 5 cut(s) 16, 335, 373, 563, 600
FaqI GGGAC 1 cut(s) 286
FatI CATG 5 cut(s) 12, 331, 369, 559, 596
FauI CCCGC 1 cut(s) 422
FauNDI CATATG 2 cut(s) 268, 925
FblI GTMKAC 3 cut(s) 5, 403, 556
Fnu4HI GCNGC 2 cut(s) 240, 444
FokI GGATG 1 cut(s) 947
FriOI GRGCYC 2 cut(s) 432, 858
Fsp4HI GCNGC 2 cut(s) 240, 444
FspBI CTAG 2 cut(s) 282, 687
GlaI GCGC 1 cut(s) 78
GluI GCNGC 2 cut(s) 240, 444
GsaI CCCAGC 1 cut(s) 548
HaeIII GGCC 5 cut(s) 20, 430, 499, 657, 896
HapII CCGG 1 cut(s) 629
HgaI GACGC 3 cut(s) 288, 675, 914
HhaI GCGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 906
Hin1II CATG 5 cut(s) 16, 335, 373, 563, 600
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 2 cut(s) 557, 904
HindII GTYRAC 2 cut(s) 557, 904
HinfI GANTC 1 cut(s) 464
HpaII CCGG 1 cut(s) 629
HphI GGTGA 3 cut(s) 23, 403, 950
Hpy166II GTNNAC 5 cut(s) 6, 404, 489, 557, 904
Hpy188I TCNGA 7 cut(s) 148, 171, 187, 261, 463, 934, 964
Hpy188III TCNNGA 4 cut(s) 303, 629, 663, 887
Hpy8I GTNNAC 5 cut(s) 6, 404, 489, 557, 904
HpyAV CCTTC 6 cut(s) 97, 101, 390, 471, 696, 757
HpyCH4III ACNGT 2 cut(s) 53, 989
HpyCH4V TGCA 6 cut(s) 89, 266, 452, 833, 929, 985
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 74, 337, 449
HpyF3I CTNAG 6 cut(s) 125, 137, 186, 258, 549, 675
Hsp92I GRCGYC 1 cut(s) 906
Hsp92II CATG 5 cut(s) 16, 335, 373, 563, 600
HspAI GCGC 1 cut(s) 77
Kpn2I TCCGGA 1 cut(s) 628
Kzo9I GATC 5 cut(s) 610, 621, 649, 844, 883
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 2 cut(s) 467, 938
MaeI CTAG 2 cut(s) 282, 687
MalI GATC 5 cut(s) 612, 623, 651, 846, 885
MboI GATC 5 cut(s) 610, 621, 649, 844, 883
MboII GAAGA 3 cut(s) 414, 515, 659
MflI RGATCY 3 cut(s) 621, 649, 844
MhlI GDGCHC 3 cut(s) 432, 858, 975
MluCI AATT 5 cut(s) 24, 90, 181, 407, 782
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 7 cut(s) 10, 181, 367, 544, 625, 701, 805
Mph1103I ATGCAT 2 cut(s) 268, 931
MroI TCCGGA 1 cut(s) 628
MseI TTAA 3 cut(s) 72, 180, 788
MslI CAYNNNNRTG 1 cut(s) 601
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 1 cut(s) 629
MspR9I CCNGG 5 cut(s) 175, 244, 348, 654, 899
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 5 cut(s) 175, 244, 348, 654, 899
MwoI GCNNNNNNNGC 4 cut(s) 53, 74, 337, 449
NdeI CATATG 2 cut(s) 268, 925
NdeII GATC 5 cut(s) 610, 621, 649, 844, 883
NlaIII CATG 5 cut(s) 16, 335, 373, 563, 600
NlaIV GGNNCC 3 cut(s) 430, 712, 768
NsiI ATGCAT 2 cut(s) 268, 931
NspI RCATGY 1 cut(s) 563
PctI GAATGC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 464
PflMI CCANNNNNTGG 1 cut(s) 347
PkrI GCNGC 2 cut(s) 241, 445
PpuMI RGGWCCY 2 cut(s) 389, 710
Psp124BI GAGCTC 1 cut(s) 858
Psp5II RGGWCCY 2 cut(s) 389, 710
Psp6I CCWGG 5 cut(s) 173, 242, 346, 652, 897
PspFI CCCAGC 1 cut(s) 544
PspGI CCWGG 5 cut(s) 173, 242, 346, 652, 897
PspN4I GGNNCC 3 cut(s) 430, 712, 768
PspOMI GGGCCC 1 cut(s) 428
PspPI GGNCC 8 cut(s) 19, 58, 389, 428, 429, 656, 710, 895
PspPPI RGGWCCY 2 cut(s) 389, 710
PstNI CAGNNNCTG 2 cut(s) 392, 898
PsuI RGATCY 3 cut(s) 621, 649, 844
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 601
SacI GAGCTC 1 cut(s) 858
SalI GTCGAC 1 cut(s) 555
SaqAI TTAA 3 cut(s) 72, 180, 788
SatI GCNGC 2 cut(s) 240, 444
Sau3AI GATC 5 cut(s) 610, 621, 649, 844, 883
Sau96I GGNCC 8 cut(s) 19, 58, 389, 428, 429, 656, 710, 895
ScrFI CCNGG 5 cut(s) 175, 244, 348, 654, 899
SduI GDGCHC 3 cut(s) 432, 858, 975
SfaNI GCATC 2 cut(s) 467, 938
SinI GGWCC 3 cut(s) 58, 389, 710
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 5 cut(s) 24, 90, 181, 407, 782
SsiI CCGC 3 cut(s) 56, 415, 443
SspMI CTAG 2 cut(s) 282, 687
SstI GAGCTC 1 cut(s) 858
StyD4I CCNGG 5 cut(s) 173, 242, 346, 652, 897
StyI CCWWGG 1 cut(s) 801
TaaI ACNGT 2 cut(s) 53, 989
TaqI TCGA 4 cut(s) 198, 438, 556, 858
TaqII GACCGA 1 cut(s) 981
TasI AATT 5 cut(s) 24, 90, 181, 407, 782
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 3 cut(s) 72, 180, 788
Tru9I TTAA 3 cut(s) 72, 180, 788
TseI GCWGC 1 cut(s) 239
TspDTI ATGAA 8 cut(s) 17, 168, 243, 285, 377, 386, 456, 534
Van91I CCANNNNNTGG 1 cut(s) 347
VpaK11BI GGWCC 3 cut(s) 58, 389, 710
XagI CCTNNNNNAGG 1 cut(s) 384
XapI RAATTY 1 cut(s) 24
XceI RCATGY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 2 cut(s) 594, 916
XmiI GTMKAC 3 cut(s) 5, 403, 556
XspI CTAG 2 cut(s) 282, 687
Zsp2I ATGCAT 2 cut(s) 268, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.