pycom12g06990

carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012107
Physical Location & Seq
Reverse (-)
7268220 .. 7270337
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGTATGGGGCAGGTGATGAAACTCCATTTACTTATCTTGAAGGAACTGGTAAAGGACCAAAGAAATGGGGACAAATTGACCCTCATTGGAAAGTTTGTGATACTGGAAAATTGCAGTCTCCAATCGATCTTCTTGACCAAAGGGTGCAGGTTTTCCCTCACTTGGGGAAATTGATAAGGGATTACAAACCAGCTCCTGCTACTGTGAAGAACCGAGGTCATGACATCACGATGAGGTGGAACGGAGATGCAGGAAAAATCAACATAAATGGGACTGATTATAGACTGTTACAATGTCATTGGCATTCACCCTCTGAACATACGTTTAATGGATCAAGGTATGCTTTGGAGTTTCATGTTATTCATTTAAGCTCTACCGGAAAGATTGCTGTAATTGGAATTGTGTACAAATATGGTCGACCTGATCCCCTCCTTTCAAAGCTATTCCACCACATAAAATCGGTTGGGAAAGAAGAGGAGGTTGACGTAGGGGTTATTAATCCTGGGGATATTAAGTTCGGAAGCAGAAAGTATTACAGATATATCGGTTCTCTTACCGTTCCTCCGTGCACCGAGGGAGTAATTTGGACAATCGTCAAAAAGGTGAGGACAGTTTCAAGGGAGCAAGTACATGCACTAAGGGAGGCTGTACATGATGGATTCGAGATGAATGCAAGGCCAACTCAAGAATTGGATGGAAGACCAGTGCTGTTATACACTCCAAGGGACAATGGAGGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase LSM

Protein Analysis

249

Amino Acids

27.9

Weight (kDa)

9.11

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 19 - 237 6e-40 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20990
fragaria_vesca FvH4_6g24590 FvH4_6g24590 FvH4_6g24590
malus_domestica MD12G1083000.v1.1 MD14G1078100.v1.1
prunus_persica Prupe.7G052800_v2.0.a1
pyrus_communis pycom12g06990 pycom14g06650
rosa_chinensis RchiOBHm_Chr3g0483901
rosa_laevigata RLG00000023253
rosa_multiflora Rmu_sc0002252.1_g000013
rosa_roxburghii Rroxscaffold_6G00398060
rosa_rugosa Rorug03G0208600
rosa_samantha Rh3AG257300 Rh3BG293700 Rh3CG292000 Rh3DG287100
rosa_wichuraiana Rw3G023260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2
Acc36I ACCTGC 2 cut(s) 2, 139
AccI GTMKAC 1 cut(s) 418
AclWI GGATC 2 cut(s) 340, 419
AfaI GTAC 3 cut(s) 407, 630, 651
AfiI CCNNNNNNNGG 2 cut(s) 163, 164
AgsI TTSAA 3 cut(s) 41, 438, 618
AjnI CCWGG 1 cut(s) 502
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
AluBI AGCT 3 cut(s) 194, 372, 442
AluI AGCT 3 cut(s) 194, 372, 442
Alw21I GWGCWC 1 cut(s) 572
Alw26I GTCTC 1 cut(s) 123
Alw44I GTGCAC 1 cut(s) 568
AlwI GGATC 2 cut(s) 340, 419
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 1 cut(s) 677
ApaLI GTGCAC 1 cut(s) 568
AseI ATTAAT 1 cut(s) 498
AspS9I GGNCC 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 26, 300, 616
AvaII GGWCC 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 572
BbsI GAAGAC 1 cut(s) 706
Bbv12I GWGCWC 1 cut(s) 572
BccI CCATC 2 cut(s) 650, 689
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 1 cut(s) 504
BcoDI GTCTC 1 cut(s) 123
BfuAI ACCTGC 2 cut(s) 2, 139
Bme1390I CCNGG 1 cut(s) 504
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 56
BmrFI CCNGG 1 cut(s) 504
BmsI GCATC 1 cut(s) 238
BoxI GACNNNNGTC 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 706
BpuEI CTTGAG 1 cut(s) 669
Bsa29I ATCGAT 1 cut(s) 126
BsaJI CCNNGG 4 cut(s) 214, 503, 573, 722
BsaWI WCCGGW 1 cut(s) 377
BsaXI ACNNNNNCTCC 2 cut(s) 547, 577
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 164
Bse1I ACTGG 3 cut(s) 52, 109, 704
BseBI CCWGG 1 cut(s) 504
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 4 cut(s) 214, 503, 573, 722
BseGI GGATG 1 cut(s) 700
BseLI CCNNNNNNNGG 2 cut(s) 163, 164
BseNI ACTGG 3 cut(s) 52, 109, 704
BseRI GAGGAG 1 cut(s) 491
BseSI GKGCMC 1 cut(s) 572
BsgI GTGCAG 1 cut(s) 167
BshFI GGCC 1 cut(s) 679
BshVI ATCGAT 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 572
BsiSI CCGG 1 cut(s) 378
BslFI GGGAC 3 cut(s) 84, 286, 740
BslI CCNNNNNNNGG 2 cut(s) 163, 164
BsmAI GTCTC 1 cut(s) 123
BsmFI GGGAC 3 cut(s) 84, 286, 740
BsmI GAATGC 2 cut(s) 304, 676
BsnI GGCC 1 cut(s) 679
Bsp1286I GDGCHC 1 cut(s) 572
Bsp1407I TGTACA 2 cut(s) 405, 649
Bsp143I GATC 3 cut(s) 127, 332, 424
BspANI GGCC 1 cut(s) 679
BspDI ATCGAT 1 cut(s) 126
BspHI TCATGA 1 cut(s) 220
BspMI ACCTGC 2 cut(s) 2, 139
BspPI GGATC 2 cut(s) 340, 419
BsrGI TGTACA 2 cut(s) 405, 649
BsrI ACTGG 3 cut(s) 52, 109, 704
BssECI CCNNGG 4 cut(s) 214, 503, 573, 722
BssMI GATC 3 cut(s) 127, 332, 424
BssT1I CCWWGG 1 cut(s) 722
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 4 cut(s) 205, 288, 559, 613
Bst6I CTCTTC 1 cut(s) 468
BstAUI TGTACA 2 cut(s) 405, 649
BstDEI CTNAG 1 cut(s) 638
BstF5I GGATG 1 cut(s) 700
BstKTI GATC 3 cut(s) 130, 335, 427
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 3 cut(s) 127, 332, 424
BstNI CCWGG 1 cut(s) 504
BstNSI RCATGY 1 cut(s) 635
BstPAI GACNNNNGTC 1 cut(s) 593
BstSCI CCNGG 1 cut(s) 502
BstSLI GKGCMC 1 cut(s) 572
BstV2I GAAGAC 1 cut(s) 706
BstXI CCANNNNNNTGG 1 cut(s) 66
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 1 cut(s) 679
BsuTUI ATCGAT 1 cut(s) 126
BtsCI GGATG 1 cut(s) 700
BtsIMutI CAGTG 1 cut(s) 711
BveI ACCTGC 2 cut(s) 2, 139
CaiI CAGNNNCTG 1 cut(s) 197
CciI TCATGA 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 56
ClaI ATCGAT 1 cut(s) 126
Csp6I GTAC 3 cut(s) 406, 629, 650
CviAII CATG 4 cut(s) 221, 356, 632, 653
CviJI RGCY 5 cut(s) 194, 372, 442, 647, 679
CviKI_1 RGCY 5 cut(s) 194, 372, 442, 647, 679
CviQI GTAC 3 cut(s) 406, 629, 650
DdeI CTNAG 1 cut(s) 638
DpnI GATC 3 cut(s) 129, 334, 426
DpnII GATC 3 cut(s) 127, 332, 424
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 722
Eco47I GGWCC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 722
ErhI CCWWGG 1 cut(s) 722
FaeI CATG 4 cut(s) 224, 359, 635, 656
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FaqI GGGAC 3 cut(s) 84, 286, 740
FatI CATG 4 cut(s) 220, 355, 631, 652
FblI GTMKAC 1 cut(s) 418
FokI GGATG 1 cut(s) 707
HaeIII GGCC 1 cut(s) 679
HapII CCGG 1 cut(s) 378
Hin1II CATG 4 cut(s) 224, 359, 635, 656
HincII GTYRAC 2 cut(s) 419, 484
HindII GTYRAC 2 cut(s) 419, 484
HinfI GANTC 1 cut(s) 660
HpaII CCGG 1 cut(s) 378
HphI GGTGA 3 cut(s) 26, 300, 616
Hpy166II GTNNAC 4 cut(s) 406, 419, 484, 570
Hpy188I TCNGA 2 cut(s) 316, 521
Hpy188III TCNNGA 6 cut(s) 38, 134, 221, 229, 664, 686
Hpy8I GTNNAC 4 cut(s) 406, 419, 484, 570
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 4 cut(s) 205, 288, 559, 613
HpyCH4IV ACGT 2 cut(s) 323, 486
HpyCH4V TGCA 6 cut(s) 115, 148, 251, 570, 635, 674
HpyF3I CTNAG 1 cut(s) 638
HpySE526I ACGT 2 cut(s) 323, 486
Hsp92II CATG 4 cut(s) 224, 359, 635, 656
Kzo9I GATC 3 cut(s) 127, 332, 424
LmnI GCTCC 2 cut(s) 199, 622
LweI GCATC 1 cut(s) 238
MaeII ACGT 2 cut(s) 323, 486
MaeIII GTNAC 1 cut(s) 288
MalI GATC 3 cut(s) 129, 334, 426
MboI GATC 3 cut(s) 127, 332, 424
MboII GAAGA 4 cut(s) 122, 220, 485, 711
MhlI GDGCHC 1 cut(s) 572
MluCI AATT 7 cut(s) 75, 110, 170, 393, 399, 582, 689
MseI TTAA 5 cut(s) 327, 368, 498, 513, 745
MslI CAYNNNNRTG 1 cut(s) 230
MspI CCGG 1 cut(s) 378
MspR9I CCNGG 1 cut(s) 504
Mva1269I GAATGC 2 cut(s) 304, 676
MvaI CCWGG 1 cut(s) 504
NdeII GATC 3 cut(s) 127, 332, 424
NlaIII CATG 4 cut(s) 224, 359, 635, 656
NspI RCATGY 1 cut(s) 635
PagI TCATGA 1 cut(s) 220
PaqCI CACCTGC 1 cut(s) 2
PctI GAATGC 2 cut(s) 304, 676
PfeI GAWTC 1 cut(s) 660
PshAI GACNNNNGTC 1 cut(s) 593
PshBI ATTAAT 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 502
PspGI CCWGG 1 cut(s) 502
PspPI GGNCC 1 cut(s) 56
PstNI CAGNNNCTG 1 cut(s) 197
RsaI GTAC 3 cut(s) 407, 630, 651
RsaNI GTAC 3 cut(s) 406, 629, 650
RseI CAYNNNNRTG 1 cut(s) 230
SalI GTCGAC 1 cut(s) 417
SaqAI TTAA 5 cut(s) 327, 368, 498, 513, 745
Sau3AI GATC 3 cut(s) 127, 332, 424
Sau96I GGNCC 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 504
SduI GDGCHC 1 cut(s) 572
SfaNI GCATC 1 cut(s) 238
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 684
SmoI CTYRAG 1 cut(s) 684
Sse9I AATT 7 cut(s) 75, 110, 170, 393, 399, 582, 689
StyD4I CCNGG 1 cut(s) 502
StyI CCWWGG 1 cut(s) 722
TaaI ACNGT 4 cut(s) 205, 288, 559, 613
TaiI ACGT 2 cut(s) 326, 489
TaqI TCGA 3 cut(s) 126, 418, 663
TasI AATT 7 cut(s) 75, 110, 170, 393, 399, 582, 689
TatI WGTACW 3 cut(s) 405, 628, 649
TfiI GAWTC 1 cut(s) 660
Tru1I TTAA 5 cut(s) 327, 368, 498, 513, 745
Tru9I TTAA 5 cut(s) 327, 368, 498, 513, 745
TscAI CASTG 1 cut(s) 711
TspDTI ATGAA 4 cut(s) 33, 344, 353, 683
TspGWI ACGGA 2 cut(s) 258, 555
TspRI CASTG 1 cut(s) 711
VneI GTGCAC 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 56
VspI ATTAAT 1 cut(s) 498
XceI RCATGY 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.