pycom12g07130

DNA damage-inducible protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007459
Physical Location & Seq
Reverse (-)
7441619 .. 7442925
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGTACTCAAGTCTCGTGCCTGTTTGGATTAGCAAGTTTGGTGCTATTATCTGGGCTATCCGTCCACTGTCTATTAGTTGTGTGGAGCAAAAGCCTAAGTTGTGTTGTTTTGGCATTAAACTCTCCAGATTGTTGAGGCTTTTAGATCAACGTTATAAGGGCATTGCTCATGGAGTTGGTCAATCAGAGATATTGGGTCGTATACATGTAGCTCCAATCAACGTAGCTGCCTATATTGGAAGTATATTTTACCCTTGTTCATTCATGGTTCTCGATGCTCCGAATATGGAGTTTCTCTTTGGGTTGGATATGCTCCGTAAGCATCAAGAGTGCATTATAGATTTGAACGAAAATGTTTTGAGAGTTGGAGGTGGAGAAGTTTCTGTACCATTTTTGCAAGAAAAAGACATCCCATCTCGTTTTCTCGATGAAGAAAGGTTCTCCAAGGAAGCATCCAGCTCAGGAGCTCCTGTAAGTCTACTCGAGTTTATGCTTTTCCTGAGGTCAAGGATTCTTGCTGTTTGCAGTTTCCGGTTCTTAAATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease UBA ubiquitin

Protein Analysis

183

Amino Acids

20.47

Weight (kDa)

8.56

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_protease PF09668 43 - 112 9.9e-21 Aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
AccI GTMKAC 2 cut(s) 202, 478
AclI AACGTT 1 cut(s) 151
AfaI GTAC 2 cut(s) 5, 387
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 1 cut(s) 346
AluBI AGCT 4 cut(s) 212, 227, 459, 467
AluI AGCT 4 cut(s) 212, 227, 459, 467
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 482
ApeKI GCWGC 1 cut(s) 227
AvaI CYCGRG 1 cut(s) 482
AxyI CCTNAGG 1 cut(s) 500
BanII GRGCYC 1 cut(s) 469
BarI GAAGNNNNNNTAC 2 cut(s) 369, 401
BauI CACGAG 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 17
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 482
BmsI GCATC 3 cut(s) 265, 331, 461
BpmI CTGGAG 1 cut(s) 109
Bpu10I CCTNAGC 1 cut(s) 460
BsaJI CCNNGG 1 cut(s) 444
BsaWI WCCGGW 1 cut(s) 531
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bse21I CCTNAGG 1 cut(s) 500
Bse3DI GCAATG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 2 cut(s) 408, 452
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 2 cut(s) 474, 491
BseXI GCAGC 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 469
BsiHKCI CYCGRG 1 cut(s) 482
BsiSI CCGG 1 cut(s) 532
BsmAI GTCTC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 482
Bsp1286I GDGCHC 1 cut(s) 469
Bsp143I GATC 1 cut(s) 145
BspCNI CTCAG 2 cut(s) 473, 492
BsrDI GCAATG 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 1 cut(s) 145
BssNAI GTATAC 1 cut(s) 203
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 444
Bst1107I GTATAC 1 cut(s) 203
Bst2BI CACGAG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 69
BstDEI CTNAG 3 cut(s) 96, 460, 500
BstF5I GGATG 2 cut(s) 408, 452
BstKTI GATC 1 cut(s) 148
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 1 cut(s) 145
BstMWI GCNNNNNNNGC 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 209
BstV1I GCAGC 1 cut(s) 214
BstZ17I GTATAC 1 cut(s) 203
Bsu36I CCTNAGG 1 cut(s) 500
BtsCI GGATG 2 cut(s) 408, 452
BtsIMutI CAGTG 1 cut(s) 65
Csp6I GTAC 2 cut(s) 4, 386
CviAII CATG 3 cut(s) 170, 206, 265
CviJI RGCY 7 cut(s) 56, 94, 139, 212, 227, 459, 467
CviKI_1 RGCY 7 cut(s) 56, 94, 139, 212, 227, 459, 467
CviQI GTAC 2 cut(s) 4, 386
DdeI CTNAG 3 cut(s) 96, 460, 500
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 467
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 467
Eco81I CCTNAGG 1 cut(s) 500
Eco88I CYCGRG 1 cut(s) 482
EcoICRI GAGCTC 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 444
EcoT22I ATGCAT 1 cut(s) 547
EcoT38I GRGCYC 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 3 cut(s) 173, 209, 268
FatI CATG 3 cut(s) 169, 205, 264
FblI GTMKAC 2 cut(s) 202, 478
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 2 cut(s) 395, 439
FriOI GRGCYC 1 cut(s) 469
Fsp4HI GCNGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 228
GsuI CTGGAG 1 cut(s) 109
HapII CCGG 1 cut(s) 532
Hin1II CATG 3 cut(s) 173, 209, 268
HinfI GANTC 1 cut(s) 511
HpaII CCGG 1 cut(s) 532
Hpy166II GTNNAC 3 cut(s) 65, 203, 479
Hpy188I TCNGA 2 cut(s) 187, 282
Hpy188III TCNNGA 6 cut(s) 126, 272, 326, 425, 462, 499
Hpy8I GTNNAC 3 cut(s) 65, 203, 479
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 2 cut(s) 151, 222
HpyCH4V TGCA 4 cut(s) 333, 397, 525, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 319
HpyF3I CTNAG 3 cut(s) 96, 460, 500
HpySE526I ACGT 2 cut(s) 151, 222
Hsp92II CATG 3 cut(s) 173, 209, 268
Kzo9I GATC 1 cut(s) 145
LmnI GCTCC 6 cut(s) 85, 217, 283, 318, 464, 472
LpnPI CCDG 8 cut(s) 33, 37, 139, 447, 469, 483, 512, 545
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 3 cut(s) 265, 331, 461
MaeII ACGT 2 cut(s) 151, 222
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 1 cut(s) 443
MhlI GDGCHC 1 cut(s) 469
MmeI TCCRAC 2 cut(s) 285, 346
MnlI CCTC 3 cut(s) 129, 362, 495
Mph1103I ATGCAT 1 cut(s) 547
MseI TTAA 2 cut(s) 117, 539
MspI CCGG 1 cut(s) 532
MwoI GCNNNNNNNGC 1 cut(s) 319
NdeII GATC 1 cut(s) 145
NlaIII CATG 3 cut(s) 173, 209, 268
NsiI ATGCAT 1 cut(s) 547
NspI RCATGY 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 482
PciI ACATGT 1 cut(s) 205
PfeI GAWTC 1 cut(s) 511
PkrI GCNGC 1 cut(s) 229
PscI ACATGT 1 cut(s) 205
PsiI TTATAA 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 469
Psp1406I AACGTT 1 cut(s) 151
PspXI VCTCGAGB 1 cut(s) 482
RsaI GTAC 2 cut(s) 5, 387
RsaNI GTAC 2 cut(s) 4, 386
SacI GAGCTC 1 cut(s) 469
SaqAI TTAA 2 cut(s) 117, 539
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 1 cut(s) 145
SduI GDGCHC 1 cut(s) 469
SetI ASST 9 cut(s) 154, 214, 225, 229, 373, 440, 461, 469, 506
SfaNI GCATC 3 cut(s) 265, 331, 461
Sfr274I CTCGAG 1 cut(s) 482
SlaI CTCGAG 1 cut(s) 482
SmlI CTYRAG 2 cut(s) 7, 482
SmoI CTYRAG 2 cut(s) 7, 482
SstI GAGCTC 1 cut(s) 469
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 2 cut(s) 154, 225
TaqI TCGA 3 cut(s) 273, 426, 483
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 1 cut(s) 511
Tru1I TTAA 2 cut(s) 117, 539
Tru9I TTAA 2 cut(s) 117, 539
TscAI CASTG 1 cut(s) 72
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 3 cut(s) 249, 253, 444
TspGWI ACGGA 2 cut(s) 50, 305
TspRI CASTG 1 cut(s) 72
XceI RCATGY 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 482
XmiI GTMKAC 2 cut(s) 202, 478
Zsp2I ATGCAT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.