pycom12g13690

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007182
Physical Location & Seq
Reverse (-)
16158270 .. 16169254
10985 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6363 bp
ATGGAAGTCGATTTGTCTCCTGAATCCATCCACATCAAGCATCGTGATCGAATCGTGCGGAGGCTTGCTCAATTGGGAGTGCCAAATGAGTTTCTTGGTGAATACCAACGAGGGTTGGTTGCTTATGTTAAGAATAACAAACACCAGATACGGGAACTGGTTTCCGCTGTTCTACCTGCTGATGAGGAAGTGGCAGAAGTTTTAAATGAAGCTAAACCAGGGAGTAAGAAACAAGGTGTAACCAGGAAAAATAGATTCCGTGAAAACATGTCGTGGTTGCAGTGGTTGATGTTTGAGGGCGAACCTGAGGCAGCCCTGAGGAGTCTCTCCAAAATGAGTGTTGGGCAGCGTGGTTTTTGCGGAGCTGTTTGGGGACATAACGACATTGCGTACAGATGCCGAACTTGTGAAAATGATCCAACCTGTGCAATTTGTGTTCCGTGTTTTCAGAATGGGAACCACAAGGATCATGATTATTCAGTTATATATACTGGTGGCGGTTGTTGTGATTGTGGTGATGTTACAGCTTGGAAGCGCGAAGGCTTTTGTTCAAAGCATAAAGGTGCAGAACAGATACAACCTCTCCCAAAGAAGATTGCAAGCATCGCAGGGCCTGTTCTTGATTGCCTGTTCGGTATATGGAAAACCAAGCTGGTTCTTGGTGAAACTATCTGGCGGGAAAATGCTAGAGCAAGTAACCAAGTTGGAGAGCGTAGAAAGGTTGAAAATGAGTTAACTTTTGTAGTGGTTGAGATGCTTCTAGAATTCTGTAAGTACAGTGAGAGTCTGCTAAGTTTTGTATCCAAGACGGTTTTAGCATCAGGGGATCTATTGGGGATTCTTGTGAGAGCAGAGAGCTTTTTGAATGACACTGTTACGAAAAGGCTGCATGAGTTGCTACTGAAACTGCTGGGAGAACCAGTTTTCAAATATGAGTTTGCCAAAGTATTTCTGAGTTATTACCCAGCCGTTGTAAGTGAAGCCATAAAGGAATTAAGTGACCATTCTTTGAAAAAGTATCCACTGCTCTCTGTATTCTCTGTACAAATCTTCACGGTGCCAACTTTAACACCTCGTCTTGTTAAAGAAATGAACCTACTGTCTATGCTGATGGGATGCTTGGAAGATATTTTCATTTCCTGTGCTGGGGATGAAGGTCGTTTACAGGTTACCAAGTGGTCAAATTTGTATGACATCACGATTCGTGTGATTGAAGATATTCGATTTGTTATGAGCCATGCTACAGTGCCCAAATATGTAACACATGACCAGCAAGATATCTCAAGAACTTGGATAAGACTCTTGGCTTTCGTGCAAGGGATGAACCCACAAAAAAGAGAAACAGGCATGCATATTGAAGAAGAAAATGAGAATATGCATCTGCCATTTGTTCTAGGTCATTCAATTGCCAACATTCATTCTTTATTGGTGGATGGGGCATTTTTTGTGGCTATTGACAAGATGGATGAAGACTCTCTTTTCAGAACATATAAGCAGGATATGGATGACAGGGATAGCCTAAGACATTCAGAAGTAGGACGGCTGTCCCAGGAAAGTTCTGCATGCAGTGCAGTGGGGAGTAGTAGTTCATTTGCGTGTGAGTCGAAGGTTTCTGAAGATAAATCAGATGCTCTTTCTGATTTGTTGATTCCACCGTCTGTGATGTGGCTAACATATGAGTGCTTGAGGGCAATTAAGAATTGGCTGGGAGTTGATAATACCTCTGGGACTCTTCTTGATGCCTCTTCTCCAAGTACCAGCAATTTCTCTGCTAGCAATTTTTCAGCATTGAAGAGAACATTATCCAAGATCAGGAAAGGAAAATATATTTTTGGTAGACTTGCCAGTTCAAGTGAAGATCATGGCAAGCAGTGTTCATCACATGGACGTAGTGATTGCAATATGAGTATTGACACCCAAAATGGCAAAAGTGCAGGGCAGGAGAGTAAATTGATGGTTACTGACGAAATTGATTCAGTTAATGTCTGCAATCCTGCAGGATTTGATGATAGTGCTATGGAAGTAGATGGTGCCATGGACTTGGATGCTCAAAGCGTTCTGAGTTTGTCTGATTGGCCAGACATAACATATGATGTTAGTTCGCAGGATATATCAGTTCACATTCCTTTACACCGTTTACTTTCCTTGCTTTTACAGAAAGCATTAAGAAGATGTTTTGGTGATGTGCCAGATCTGGCTAGTGTTACTTCTACAAATTCATCATCAGCTATTTTCACTGACTTCTTCGGAAATATTCTGGGAGGCTGCCACCCATTTGGATTTTCTGCCTTTGTTATGGAGCATCCCCTTCGGATCAGAGTATTTTGTGCTGAGGTTCATGCTGGAATGTGGCGGAAAAATGGGGATGCCGCCCTGTTATCTTGTGAGTGGTATCGTTCAGTTCGCTGGTCTGAACAGGGTTTGGAGCTTGATCTGTTTCTGCTTCAGTGCTGTGCTGCCTTGGCTCCAGCTGATCCTTATGTTAACAGAATTGTAGAACGCTTTGGCCTGTCCAGCTACCTTTCTTTGAACCTTGAGCGCTCTAGTGAATATGAAACTATTCTAGTGCAAGAAATGCTCACTCTTATTATACAAATAATCAAAGAAAGGCGATTTTGTGGTCTTACTAAAGCTGAAAGTTTGAAAAGAGAGTTGGTTCATAAATTAGCCGTTGCAGATGGCACTCATAGTCAATTGGTGAAATCTCTTCCGCGTGATCTGTCCAAGTCTGATCAGCTACATGGAATTTTGGAATCCATTGCCATGTACTCCAATCCCTCTGGCTTTAATCAGGGGACTTATTCACTACAATGGACATTTTGGAAAGAAATGGATTTGTACTACCCTCGTTGGAACTCAAGGGATCTGCAAGCTGCAGAAGAAAGATACTTGCGTTTTCGTGGTGTTTGTGCATTGACCACTCAGCTACCCAGATGGACTAAGATTTATTCTCCTTTCAAGGGGGTAGCTAGAATAGCTACATGTAAATCAGTGCTTCAAATTATCCGTGCTGTGCTGTTTTATGCTGTTTTTTCTGATAAATCAAATGATTCACGAGCTCCCGATAGTGTTCTCTTAACTGCATTGCACTTGCTCTCATTAGCATTAGACATTTGTTCTCAACATAAGGAAGCTGGTGATAAGTCTTGTTATGATGGAGATGCAATTCCAATGCTAGCTTTTGCTGGGGAAGAAATTACTGAAGGACGATACTTTGGTGCTGGTCAACAAAGCTTACTGTCACTTCTTGTTATCTCGATGAGGATGTATAAGAAAGAGAATGTCCACAATTGTTTGGAAGCTGGAAGTTGCGATCTCTCTTCTTTAATTGGAAGCTTATTAAAGAAGTTTGTAGAGATTGATTCCGGATGTATGACCGTATTACAACAACTTGCACCTGAAGTGATTGGTCACGTATCACAATCTTCTTTAAATGGTGATGCTAAGACCTCAGGTTCAATTTCTGACTCTGAGAAGCGCAAGGCAAAAGCTAGAGAGAGACAGGCTGCCATATTGGAAAAAATGAGAGCCGAACAATCTAAGTTTATGGCCAGTGTTAATTCCACTGTGGATGGGGGTTCAAAATCTGAGCAAGAGGTTTGTAAACTCAATATTGAAGATGACTCGGAAGAATCTGAACAAGTTGTTTGCTCTCTTTGTCATGATCCCAGTTCCAGAAATCCCATATCCTACTTGGTTTTACTTCAGAAATCTAGACTCTTAAGTTTCATAGAAAGAGGTCCCCTGTCATGGGAGCAACCACCTTGGACAGACAAGGAGCACATCTCCATTACTACAAACAAGGTGAGTGATCAATCTGGATCAAGCACTTTATCAAGTGGTTCAGGACCAGAACTGGTGCCATCTTATTCTTGCAGACAGTTGGTTCAGAATGCAATCGCCAAATTTGCCTGTCATAGACAACCTAGGGATGTTGCTGCTCTTATAAATTTTCTCAAGGGCCAGTTCCATGAATTAAGGAATATCCAAGTGCCACGTGAATCAAATGATGATATGGTGAAGACTTTATTTACATTTGAGACAATGGAAGACGATATGTACATCTCCATCCAAAGGGAAACGCGCGATAAGATACTTCATTCTAATCCTTTAGAGGATAAGGGATTTTCAACTCCTGAAGGGGATCAAGAAGAAACTAAGCATGCTGAATTTATGTTACTTGGAACATATATAGCTGCTCTTTCTAGAGAGACAACAGAGATTCCTTCATCTTCTGAAAGTGCTCCTAACGAAAGGGTGCCAGTAGATTCTTCTTGTCTTTCAGCGCGTGACAGATTTGGCCCGACAGATTGTGATGGAATTTATCTTTCTTCCTGTGGACATGCTGTACATCAGGGATGCCTTGATCGTTATTTATCTTCGTTGAAGGAAAGGTATCTCAGAAGAATTGTTTTTGAAGGGGGACATATTGCAGATCCGGATAAGGGGGAGTTTCTCTGCCCTGTATGCCGTCGACTTGCAAATTCTGTCTTGCCCGCATTGCCAGGGTTCCTCCAGGTCACCAAGGAGCCCTTGCATTCAGGTGTTAATTCATCACATGCTACTGGTCCATCGGTCAAATCAGTTGACAAAATTAATTCTCTTCAGCTTCAACAAGGGTTGGCTCTCTTGCAATCTGCAGCAGAGGCATCTGGGAAGGTTGGAAGTCTTAAAGGTTTCCCCCTTCAGAGATGTGGGAGAATGACCTCAGACCTTGAACCAATTTTTCGATTGCTCAGTAAGATGTATTTTCCGACTAAACAGGATAAGATATCAGGATCTGCCAGGGTTAACCACCCAATGCTTATGTGGGACAAAATTAAGTACTCTCTAGTATCAACAGAAATTGCTGCTCGTTCTGATGGGAAATATGCAACACCAAGCTATGGCCTTAATGCGTTGTATAAGGAACTTGAATCAAGTAGATTTATTTTGTCCTTGTTGCTGAAAATAGTCCAAAGCAGAAGAAAGAATTCTCTTCATGTCCTTCAGAGATTTATAGGAATTCAAAGCTTTACAAAATCTATTCTATTTGGAGTTTCCATAGACGTTCGCAATGAAACATGTGGACAAGGTGCTATGCTGCGTATCTTGGAACATGCTGATCTAGCGGTATCGTATCCTGATATTCAGTTCTGGAGTCGAGCTTCTGATCCTGTCCTTGCTTGCGATCCTTTTTCATCATTGATGTGGGTTCTTTTTTGTCTTCCATACCGATTTTTATCATGTGAAGATTCATTGTTGTCCCTAGTGCACTTATTTTATGTTGTCTCTGTAGTTCAGGGCATAATGACATATTGTGGGAAAAATCAATGTGACATTAGTGGATTAGGTATTGATGATTGCCTGGTTACTGACCTCTCTAAACTTATGGAAGAATCTGGAGCCACTCAGCAATTTTTCGTTTCAAATTATATTGGCTCTTCTCCTAATATCAAGAACATAGTTCGTAGCCTGAGTTTCCCTTATTTGCGGAGGTGTGCATTGCTGTTGAAGCTACTGAACTCCTGTTCCCGAGTACCATTCCATGAAAGGTATAATGTCTTGGATAGATCTCGTGCCATTGGTGACATGATGGACACCACTTATGTTGCACTAGTGGAGCTAAATGATGTTCAAGAAATAGAAAGCATGTTAAAGATTCCTTCTCTGGATGTTATCTTTAAGGATAATGTGGTACGTTCAATAGCTCAGAAATGGTTCCATCATTTTCGGAAGGAGTTTGAAGTCCAAAGATTTCAAGGAAGCATGCACTGTATCCCTGCAGTTCCCTTCCAATTGATGCGTCTTCCCCAAGTTTACCAGGACCTATTGCAGAGGTACATAAAACAGCGTTGTCCTGATTGCAAAAATATATTTGATGACCCTGCATTGTGCTTGCTTTGTGGGAGATTATGTTCTCCAAGCTGGAAGTCATGTTGCAGGGAAAGTGGATGCCAAACTCATGCAGTGTCCTGTGGTTCTGGTACTGGTGTATTTCTGTTGATCAGGAGGACAACAATTCTGCTGCAGAGATCTGCACGGCAAGCTCCCTGGCCATCGCCCTACTTGGATGCTTTTGGAGAAGAGGATGTTGAAATGCACAGAGGAAAGCCACTGTACTTGAACGAGGAACGCTATGCAGCTTTAACTTACTTGGTTGCTTCTCATGGCCTGGATCGAAGCTCCAAGCAGAGAATATCCACTTCTGACAATAAATCTGGACCTTGGATCAATGGTTCAGTTAACTGCACATTGAGATGCTTGGGGTATTCCTCTCAAGAGTTTGAAAATTTGGTCCCAAGGGAAGACCAGTTTGTCGGCAGCAGGTTGTTTCATCCGTTGTTTTATGCATGTACAGTTCGATATGATGCTGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

2121

Amino Acids

237.58

Weight (kDa)

6.37

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 120 - 186 6.7e-20 Putative zinc finger in N-recognin (UBR box)
WHD_UBR1 PF22960 881 - 965 7.4e-19 E3 ubiquitin-protein ligase UBR1-like, winged-helix domain
PRT6_C PF18995 1581 - 2048 1.6e-91 Proteolysis_6 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3947
AasI GACNNNNNNGTC 1 cut(s) 6296
Acc36I ACCTGC 2 cut(s) 184, 6297
AccB1I GGYRCC 4 cut(s) 1057, 2030, 3859, 4258
AccB7I CCANNNNNTGG 1 cut(s) 4886
AccI GTMKAC 2 cut(s) 1837, 4474
AccII CGCG 5 cut(s) 537, 2713, 4084, 4086, 4288
AccIII TCCGGA 2 cut(s) 3365, 4438
AcoI YGGCCR 3 cut(s) 2075, 3549, 6035
AcuI CTGAAG 9 cut(s) 1635, 2429, 3222, 3420, 3689, 4158, 4589, 4670, 4973
AcvI CACGTG 1 cut(s) 3998
AfeI AGCGCT 1 cut(s) 2540
AfiI CCNNNNNNNGG 9 cut(s) 151, 1146, 1812, 2960, 3750, 3751, 4074, 4445, 4886
AflII CTTAAG 1 cut(s) 3721
AflIII ACRYGT 3 cut(s) 267, 2981, 5063
AhlI ACTAGT 1 cut(s) 5596
AjuI GAANNNNNNNTTGG 2 cut(s) 2636, 2668
AloI GAACNNNNNNTCC 8 cut(s) 1570, 1602, 5495, 5527, 5881, 5913, 6202, 6234
Alw21I GWGCWC 4 cut(s) 3061, 3783, 4246, 5256
Alw26I GTCTC 6 cut(s) 21, 329, 3493, 4034, 4205, 5275
Alw44I GTGCAC 1 cut(s) 5252
AlwNI CAGNNNCTG 2 cut(s) 614, 4781
Ama87I CYCGRG 1 cut(s) 5514
Aor13HI TCCGGA 2 cut(s) 3365, 4438
Aor51HI AGCGCT 1 cut(s) 2540
AoxI GGCC 9 cut(s) 611, 2075, 2506, 3549, 3961, 4300, 4888, 6035, 6151
ApaLI GTGCAC 1 cut(s) 5252
AseI ATTAAT 1 cut(s) 4596
Asp700I GAANNNNTTC 4 cut(s) 1218, 2559, 4972, 5699
AspA2I CCTAGG 1 cut(s) 3926
AspLEI GCGC 5 cut(s) 537, 2541, 3480, 4086, 4288
AspS9I GGNCC 9 cut(s) 611, 3740, 3848, 3961, 4301, 4568, 5806, 6203, 6277
AsuNHI GCTAGC 2 cut(s) 1772, 3175
AvaI CYCGRG 1 cut(s) 5514
AvaII GGWCC 6 cut(s) 3740, 3848, 4568, 5806, 6203, 6277
AvrII CCTAGG 1 cut(s) 3926
AxyI CCTNAGG 3 cut(s) 306, 317, 3451
BaeGI GKGCMC 2 cut(s) 1251, 5256
BaeI ACNNNNGTAYC 6 cut(s) 2878, 2911, 3202, 3235, 5958, 5991
BalI TGGCCA 3 cut(s) 2077, 3551, 6037
BanI GGYRCC 4 cut(s) 1057, 2030, 3859, 4258
BanII GRGCYC 2 cut(s) 3061, 4533
BarI GAAGNNNNNNTAC 2 cut(s) 1146, 1178
BauI CACGAG 2 cut(s) 3054, 5556
BbrPI CACGTG 1 cut(s) 3998
BbsI GAAGAC 6 cut(s) 1476, 4028, 4056, 5198, 5780, 6294
Bbv12I GWGCWC 4 cut(s) 3061, 3783, 4246, 5256
BbvCI CCTCAGC 1 cut(s) 2331
BceAI ACGGC 5 cut(s) 953, 1556, 2654, 4455, 6038
BcgI CGANNNNNNTGC 6 cut(s) 29, 63, 868, 902, 2291, 2325
BciVI GTATCC 4 cut(s) 811, 1029, 5130, 5768
BclI TGATCA 3 cut(s) 2731, 3811, 5985
BcoDI GTCTC 6 cut(s) 21, 329, 3493, 4034, 4205, 5275
BcuI ACTAGT 1 cut(s) 5596
BfmI CTRYAG 7 cut(s) 1242, 1995, 2874, 4638, 5274, 5763, 6008
BfoI RGCGCY 1 cut(s) 2542
BfrI CTTAAG 1 cut(s) 3721
BfuAI ACCTGC 2 cut(s) 184, 6297
BfuI GTATCC 4 cut(s) 811, 1029, 5130, 5768
BglII AGATCT 3 cut(s) 2191, 5552, 6014
BlnI CCTAGG 1 cut(s) 3926
BmcAI AGTACT 1 cut(s) 4826
Bme18I GGWCC 6 cut(s) 3740, 3848, 4568, 5806, 6203, 6277
BmeT110I CYCGRG 1 cut(s) 5514
BmgT120I GGNCC 9 cut(s) 611, 3740, 3848, 3961, 4301, 4568, 5806, 6203, 6277
BmrI ACTGGG 1 cut(s) 3663
BmtI GCTAGC 2 cut(s) 1776, 3179
BmuI ACTGGG 1 cut(s) 3663
BoxI GACNNNNGTC 1 cut(s) 1543
BpiI GAAGAC 6 cut(s) 1476, 4028, 4056, 5198, 5780, 6294
BplI GAGNNNNNCTC 4 cut(s) 52, 84, 3770, 3802
BpmI CTGGAG 4 cut(s) 2451, 4499, 5158, 5403
Bpu10I CCTNAGC 1 cut(s) 2331
BpuEI CTTGAG 6 cut(s) 1267, 1705, 2555, 2842, 3941, 6243
BsaAI YACGTR 2 cut(s) 3415, 3998
BsaBI GATNNNNATC 1 cut(s) 5221
BsaWI WCCGGW 2 cut(s) 3365, 4438
BsaXI ACNNNNNCTCC 8 cut(s) 567, 597, 906, 936, 1570, 1600, 5881, 5911
Bsc4I CCNNNNNNNGG 9 cut(s) 151, 1146, 1812, 2960, 3750, 3751, 4074, 4445, 4886
Bse21I CCTNAGG 3 cut(s) 306, 317, 3451
Bse3DI GCAATG 6 cut(s) 384, 2757, 3083, 4499, 5062, 5483
Bse8I GATNNNNATC 1 cut(s) 5221
BseAI TCCGGA 2 cut(s) 3365, 4438
BseJI GATNNNNATC 1 cut(s) 5221
BseLI CCNNNNNNNGG 9 cut(s) 151, 1146, 1812, 2960, 3750, 3751, 4074, 4445, 4886
BseMI GCAATG 6 cut(s) 384, 2757, 3083, 4499, 5062, 5483
BseRI GAGGAG 1 cut(s) 334
BseSI GKGCMC 2 cut(s) 1251, 5256
BseYI CCCAGC 5 cut(s) 910, 964, 1145, 1705, 3185
BsgI GTGCAG 5 cut(s) 585, 1590, 1953, 6003, 6214
Bsh1236I CGCG 5 cut(s) 537, 2713, 4084, 4086, 4288
BshFI GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4302, 4890, 6037, 6153
BshNI GGYRCC 4 cut(s) 1057, 2030, 3859, 4258
BsiHKAI GWGCWC 4 cut(s) 3061, 3783, 4246, 5256
BsiHKCI CYCGRG 1 cut(s) 5514
BsiSI CCGG 2 cut(s) 3366, 4439
BslFI GGGAC 9 cut(s) 387, 1531, 1741, 2809, 3726, 4437, 4826, 5230, 6263
BslI CCNNNNNNNGG 9 cut(s) 151, 1146, 1812, 2960, 3750, 3751, 4074, 4445, 4886
BsmAI GTCTC 6 cut(s) 21, 329, 3493, 4034, 4205, 5275
BsmFI GGGAC 9 cut(s) 387, 1531, 1741, 2809, 3726, 4437, 4826, 5230, 6263
BsmI GAATGC 2 cut(s) 3898, 4537
BsnI GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4302, 4890, 6037, 6153
BsoBI CYCGRG 1 cut(s) 5514
Bsp1286I GDGCHC 6 cut(s) 1251, 3061, 3783, 4246, 4533, 5256
Bsp13I TCCGGA 2 cut(s) 3365, 4438
Bsp1407I TGTACA 4 cut(s) 1042, 4059, 4348, 6335
Bsp19I CCATGG 1 cut(s) 2034
BspANI GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4302, 4890, 6037, 6153
BspEI TCCGGA 2 cut(s) 3365, 4438
BspFNI CGCG 5 cut(s) 537, 2713, 4084, 4086, 4288
BspHI TCATGA 2 cut(s) 469, 3661
BspMAI CTGCAG 5 cut(s) 1999, 2878, 4642, 5767, 6012
BspMI ACCTGC 2 cut(s) 184, 6297
BspOI GCTAGC 2 cut(s) 1776, 3179
BspQI GCTCTTC 1 cut(s) 5428
BspT107I GGYRCC 4 cut(s) 1057, 2030, 3859, 4258
BspTI CTTAAG 1 cut(s) 3721
BsrDI GCAATG 6 cut(s) 384, 2757, 3083, 4499, 5062, 5483
BsrGI TGTACA 4 cut(s) 1042, 4059, 4348, 6335
BssSI CACGAG 2 cut(s) 3054, 5556
BssT1I CCWWGG 7 cut(s) 2034, 2460, 3764, 3926, 4524, 6206, 6281
Bst2BI CACGAG 2 cut(s) 3054, 5556
Bst6I CTCTTC 9 cut(s) 1737, 1750, 1787, 2712, 3325, 4608, 4983, 5428, 6060
BstAFI CTTAAG 1 cut(s) 3721
BstAPI GCANNNNNTGC 3 cut(s) 895, 1568, 2575
BstAUI TGTACA 4 cut(s) 1042, 4059, 4348, 6335
BstBAI YACGTR 2 cut(s) 3415, 3998
BstDSI CCRYGG 1 cut(s) 2034
BstEII GGTNACC 2 cut(s) 1168, 4519
BstFNI CGCG 5 cut(s) 537, 2713, 4084, 4086, 4288
BstH2I RGCGCY 1 cut(s) 2542
BstHHI GCGC 5 cut(s) 537, 2541, 3480, 4086, 4288
BstMAI GTCTC 6 cut(s) 21, 329, 3493, 4034, 4205, 5275
BstPAI GACNNNNGTC 1 cut(s) 1543
BstPI GGTNACC 2 cut(s) 1168, 4519
BstSFI CTRYAG 7 cut(s) 1242, 1995, 2874, 4638, 5274, 5763, 6008
BstSLI GKGCMC 2 cut(s) 1251, 5256
BstUI CGCG 5 cut(s) 537, 2713, 4084, 4086, 4288
BstV2I GAAGAC 6 cut(s) 1476, 4028, 4056, 5198, 5780, 6294
BstX2I RGATCY 7 cut(s) 826, 2191, 2863, 4435, 4778, 5552, 6014
BstXI CCANNNNNNTGG 2 cut(s) 2041, 2276
BstYI RGATCY 7 cut(s) 826, 2191, 2863, 4435, 4778, 5552, 6014
Bsu36I CCTNAGG 3 cut(s) 306, 317, 3451
BsuI GTATCC 4 cut(s) 811, 1029, 5130, 5768
BsuRI GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4302, 4890, 6037, 6153
BtgI CCRYGG 1 cut(s) 2034
BtgZI GCGATG 2 cut(s) 589, 6024
BtsI GCAGTG 6 cut(s) 287, 1022, 1573, 1578, 1877, 5955
BveI ACCTGC 2 cut(s) 184, 6297
CaiI CAGNNNCTG 2 cut(s) 614, 4781
CciI TCATGA 2 cut(s) 469, 3661
CfoI GCGC 5 cut(s) 537, 2541, 3480, 4086, 4288
Cfr13I GGNCC 9 cut(s) 611, 3740, 3848, 3961, 4301, 4568, 5806, 6203, 6277
CseI GACGC 1 cut(s) 5774
CspCI CAANNNNNGTGG 4 cut(s) 5572, 5607, 6084, 6119
DraI TTTAAA 2 cut(s) 204, 3432
DrdI GACNNNNNNGTC 1 cut(s) 6296
DseDI GACNNNNNNGTC 1 cut(s) 6296
EaeI YGGCCR 3 cut(s) 2075, 3549, 6035
Eam1104I CTCTTC 9 cut(s) 1737, 1750, 1787, 2712, 3325, 4608, 4983, 5428, 6060
EarI CTCTTC 9 cut(s) 1737, 1750, 1787, 2712, 3325, 4608, 4983, 5428, 6060
EciI GGCGGA 1 cut(s) 2368
Ecl136II GAGCTC 1 cut(s) 3059
Eco130I CCWWGG 7 cut(s) 2034, 2460, 3764, 3926, 4524, 6206, 6281
Eco24I GRGCYC 2 cut(s) 3061, 4533
Eco32I GATATC 2 cut(s) 1279, 4773
Eco47I GGWCC 6 cut(s) 3740, 3848, 4568, 5806, 6203, 6277
Eco47III AGCGCT 1 cut(s) 2540
Eco53kI GAGCTC 1 cut(s) 3059
Eco57I CTGAAG 9 cut(s) 1635, 2429, 3222, 3420, 3689, 4158, 4589, 4670, 4973
Eco72I CACGTG 1 cut(s) 3998
Eco81I CCTNAGG 3 cut(s) 306, 317, 3451
Eco88I CYCGRG 1 cut(s) 5514
Eco91I GGTNACC 2 cut(s) 1168, 4519
EcoICRI GAGCTC 1 cut(s) 3059
EcoO109I RGGNCCY 3 cut(s) 611, 3740, 5806
EcoO65I GGTNACC 2 cut(s) 1168, 4519
EcoRI GAATTC 3 cut(s) 764, 4972, 5004
EcoRV GATATC 2 cut(s) 1279, 4773
EcoT14I CCWWGG 7 cut(s) 2034, 2460, 3764, 3926, 4524, 6206, 6281
EcoT22I ATGCAT 3 cut(s) 1353, 1380, 6334
EcoT38I GRGCYC 2 cut(s) 3061, 4533
ErhI CCWWGG 7 cut(s) 2034, 2460, 3764, 3926, 4524, 6206, 6281
FalI AAGNNNNNCTT 4 cut(s) 1461, 1493, 4517, 4549
FaqI GGGAC 9 cut(s) 387, 1531, 1741, 2809, 3726, 4437, 4826, 5230, 6263
FauI CCCGC 2 cut(s) 669, 4504
FauNDI CATATG 2 cut(s) 1675, 2089
FbaI TGATCA 3 cut(s) 2731, 3811, 5985
FblI GTMKAC 2 cut(s) 1837, 4474
FriOI GRGCYC 2 cut(s) 3061, 4533
GlaI GCGC 5 cut(s) 536, 2540, 3479, 4085, 4287
GsaI CCCAGC 5 cut(s) 914, 968, 1149, 1709, 3189
GsuI CTGGAG 4 cut(s) 2451, 4499, 5158, 5403
HaeII RGCGCY 1 cut(s) 2542
HaeIII GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4302, 4890, 6037, 6153
HapII CCGG 2 cut(s) 3366, 4439
HgaI GACGC 1 cut(s) 5774
HhaI GCGC 5 cut(s) 537, 2541, 3480, 4086, 4288
Hin6I GCGC 5 cut(s) 535, 2539, 3478, 4084, 4286
HinP1I GCGC 5 cut(s) 535, 2539, 3478, 4084, 4286
HincII GTYRAC 7 cut(s) 735, 2485, 3227, 4475, 4588, 4792, 6226
HindII GTYRAC 7 cut(s) 735, 2485, 3227, 4475, 4588, 4792, 6226
HindIII AAGCTT 3 cut(s) 3232, 3334, 5011
HpaI GTTAAC 4 cut(s) 735, 2485, 4792, 6226
HpaII CCGG 2 cut(s) 3366, 4439
Hpy99I CGWCG 1 cut(s) 4476
HpyCH4IV ACGT 5 cut(s) 1888, 3414, 3997, 5049, 5680
HpySE526I ACGT 5 cut(s) 1888, 3414, 3997, 5049, 5680
HspAI GCGC 5 cut(s) 535, 2539, 3478, 4084, 4286
Kpn2I TCCGGA 2 cut(s) 3365, 4438
Ksp22I TGATCA 3 cut(s) 2731, 3811, 5985
KspAI GTTAAC 4 cut(s) 735, 2485, 4792, 6226
LguI GCTCTTC 1 cut(s) 5428
MaeII ACGT 5 cut(s) 1888, 3414, 3997, 5049, 5680
MfeI CAATTG 5 cut(s) 71, 1404, 2693, 3289, 5777
MflI RGATCY 7 cut(s) 826, 2191, 2863, 4435, 4778, 5552, 6014
MhlI GDGCHC 6 cut(s) 1251, 3061, 3783, 4246, 4533, 5256
MlsI TGGCCA 3 cut(s) 2077, 3551, 6037
MluNI TGGCCA 3 cut(s) 2077, 3551, 6037
MmeI TCCRAC 5 cut(s) 443, 685, 2831, 4642, 4778
Mox20I TGGCCA 3 cut(s) 2077, 3551, 6037
Mph1103I ATGCAT 3 cut(s) 1353, 1380, 6334
MroI TCCGGA 2 cut(s) 3365, 4438
MroXI GAANNNNTTC 4 cut(s) 1218, 2559, 4972, 5699
MscI TGGCCA 3 cut(s) 2077, 3551, 6037
MslI CAYNNNNRTG 8 cut(s) 561, 1594, 1678, 2762, 2809, 4542, 5188, 6238
Msp20I TGGCCA 3 cut(s) 2077, 3551, 6037
MspA1I CMGCKG 2 cut(s) 167, 2471
MspCI CTTAAG 1 cut(s) 3721
MspI CCGG 2 cut(s) 3366, 4439
MunI CAATTG 5 cut(s) 71, 1404, 2693, 3289, 5777
Mva1269I GAATGC 2 cut(s) 3898, 4537
MvnI CGCG 5 cut(s) 537, 2713, 4084, 4086, 4288
NcoI CCATGG 1 cut(s) 2034
NdeI CATATG 2 cut(s) 1675, 2089
NheI GCTAGC 2 cut(s) 1772, 3175
NmuCI GTSAC 7 cut(s) 998, 3240, 3410, 4289, 4519, 5315, 5567
NsiI ATGCAT 3 cut(s) 1353, 1380, 6334
PaeI GCATGC 4 cut(s) 1351, 1566, 4166, 5752
PagI TCATGA 2 cut(s) 469, 3661
PciI ACATGT 3 cut(s) 267, 2981, 5063
PciSI GCTCTTC 1 cut(s) 5428
PctI GAATGC 2 cut(s) 3898, 4537
PdmI GAANNNNTTC 4 cut(s) 1218, 2559, 4972, 5699
PflMI CCANNNNNTGG 1 cut(s) 4886
PmaCI CACGTG 1 cut(s) 3998
PmlI CACGTG 1 cut(s) 3998
Ppu21I YACGTR 2 cut(s) 3415, 3998
PpuMI RGGWCCY 2 cut(s) 3740, 5806
PscI ACATGT 3 cut(s) 267, 2981, 5063
PshAI GACNNNNGTC 1 cut(s) 1543
PshBI ATTAAT 1 cut(s) 4596
PsiI TTATAA 1 cut(s) 3947
Psp124BI GAGCTC 1 cut(s) 3061
Psp5II RGGWCCY 2 cut(s) 3740, 5806
PspCI CACGTG 1 cut(s) 3998
PspEI GGTNACC 2 cut(s) 1168, 4519
PspFI CCCAGC 5 cut(s) 910, 964, 1145, 1705, 3185
PspPI GGNCC 9 cut(s) 611, 3740, 3848, 3961, 4301, 4568, 5806, 6203, 6277
PspPPI RGGWCCY 2 cut(s) 3740, 5806
PstI CTGCAG 5 cut(s) 1999, 2878, 4642, 5767, 6012
PstNI CAGNNNCTG 2 cut(s) 614, 4781
PsuI RGATCY 7 cut(s) 826, 2191, 2863, 4435, 4778, 5552, 6014
PvuII CAGCTG 1 cut(s) 2471
RseI CAYNNNNRTG 8 cut(s) 561, 1594, 1678, 2762, 2809, 4542, 5188, 6238
SacI GAGCTC 1 cut(s) 3061
SalI GTCGAC 1 cut(s) 4473
SapI GCTCTTC 1 cut(s) 5428
Sau96I GGNCC 9 cut(s) 611, 3740, 3848, 3961, 4301, 4568, 5806, 6203, 6277
SbfI CCTGCAGG 1 cut(s) 1999
ScaI AGTACT 1 cut(s) 4826
SdaI CCTGCAGG 1 cut(s) 1999
SduI GDGCHC 6 cut(s) 1251, 3061, 3783, 4246, 4533, 5256
SfcI CTRYAG 7 cut(s) 1242, 1995, 2874, 4638, 5274, 5763, 6008
SinI GGWCC 6 cut(s) 3740, 3848, 4568, 5806, 6203, 6277
SmiMI CAYNNNNRTG 8 cut(s) 561, 1594, 1678, 2762, 2809, 4542, 5188, 6238
SmlI CTYRAG 7 cut(s) 1282, 1684, 2534, 2857, 3721, 3956, 6258
SmoI CTYRAG 7 cut(s) 1282, 1684, 2534, 2857, 3721, 3956, 6258
SpeI ACTAGT 1 cut(s) 5596
SphI GCATGC 4 cut(s) 1351, 1566, 4166, 5752
Sse8387I CCTGCAGG 1 cut(s) 1999
SspI AATATT 2 cut(s) 2254, 3613
SstI GAGCTC 1 cut(s) 3061
StyI CCWWGG 7 cut(s) 2034, 2460, 3764, 3926, 4524, 6206, 6281
TaiI ACGT 5 cut(s) 1891, 3417, 4000, 5052, 5683
TaqII GACCGA 1 cut(s) 4564
TatI WGTACW 9 cut(s) 774, 1042, 2766, 2838, 4059, 4348, 4824, 6099, 6335
TauI GCSGC 1 cut(s) 2372
TseFI GTSAC 7 cut(s) 998, 3240, 3410, 4289, 4519, 5315, 5567
Tsp45I GTSAC 7 cut(s) 998, 3240, 3410, 4289, 4519, 5315, 5567
TspGWI ACGGA 4 cut(s) 248, 429, 2996, 6309
Van91I CCANNNNNTGG 1 cut(s) 4886
Vha464I CTTAAG 1 cut(s) 3721
VneI GTGCAC 1 cut(s) 5252
VpaK11BI GGWCC 6 cut(s) 3740, 3848, 4568, 5806, 6203, 6277
VspI ATTAAT 1 cut(s) 4596
XbaI TCTAGA 3 cut(s) 760, 3713, 4205
XcmI CCANNNNNNNNNTGG 2 cut(s) 3691, 4806
XmaJI CCTAGG 1 cut(s) 3926
XmiI GTMKAC 2 cut(s) 1837, 4474
XmnI GAANNNNTTC 4 cut(s) 1218, 2559, 4972, 5699
ZrmI AGTACT 1 cut(s) 4826
Zsp2I ATGCAT 3 cut(s) 1353, 1380, 6334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.