pycom12g14180

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016421
Physical Location & Seq
Forward (+)
16785416 .. 16786290
875 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g14180.2

Sequence Viewer

Length: 726 bp
ATGCTAGGCCTAAGTAGTCGGGACTATGGGGTAATAAATTGTTGGTTCATTAACTGTATGCATTCATCTAATGTTTACTGGACAAAAGATTTGGATCCTATCACCAAGCACTTCTATTGTGTTTTTTGTAACCACAAATGCACGGGTTCAATTTCTAGATTCAAACATCACTTGGGTGGTGTAAGTTCGGGAGGAATGATAAGTTGTAAAAAAGTCCCACCCAATGTGAAGGAAGAATGCTTTGCTAATGTTATGGGAACTAAAGAAAAGAGGAAAGCTAGTCATGAGTTGTGTCGTGGTGGTGGAGAAGAAAGTGAAAGTGTCAATATGGGCACAACAAGGACACCATATGTAAGTAGTGGGAATGGGAGTGCGAGTGCCACACATTCACAACAAACCGATTCTTTTCTACCACCTAGGTCAAGGGGACCACTTGATAGGTATGTTACATCGGAAGCTCGTCAAACCACGTTGAATACACCTTTCAAAAAAGAGGAAAGAAGGCAAGCATCCCGAGCGGCAAACAATGAATATTATGTTAATGCAATGAAATTGGTATCCAATTTTGGTCCCGGCTTTGATGCTCCTACAAGCCATGAATATAGAACTTGGATGCTCAAAGAAGAAGTTGATGATGTACAAAGTATGATGAAAGAACATCAAAAGGCTTGGAAGAGATATGGATGCACTATTATGTCGGATGGATGGTCGAAAGTAGGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

242

Amino Acids

27.11

Weight (kDa)

9.0

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 518
AciI CCGC 1 cut(s) 518
AclWI GGATC 2 cut(s) 89, 102
AfaI GTAC 1 cut(s) 639
AgsI TTSAA 4 cut(s) 150, 163, 475, 487
AleI CACNNNNGTG 1 cut(s) 174
AluBI AGCT 2 cut(s) 278, 458
AluI AGCT 2 cut(s) 278, 458
AlwI GGATC 2 cut(s) 89, 102
Ama87I CYCGRG 1 cut(s) 513
AoxI GGCC 1 cut(s) 7
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
AspA2I CCTAGG 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 428, 569
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 2 cut(s) 428, 569
AvrII CCTAGG 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 335
BamHI GGATCC 1 cut(s) 94
BccI CCATC 2 cut(s) 695, 699
BciVI GTATCC 1 cut(s) 568
BcnI CCSGG 1 cut(s) 573
BfaI CTAG 4 cut(s) 5, 156, 279, 417
BfuI GTATCC 1 cut(s) 568
BisI GCNGC 1 cut(s) 519
BlnI CCTAGG 1 cut(s) 416
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 1 cut(s) 573
Bme18I GGWCC 2 cut(s) 428, 569
BmeT110I CYCGRG 1 cut(s) 513
BmgT120I GGNCC 2 cut(s) 428, 569
BmiI GGNNCC 3 cut(s) 96, 429, 571
BmrFI CCNGG 1 cut(s) 573
BmsI GCATC 4 cut(s) 518, 571, 603, 674
BpuMI CCSGG 1 cut(s) 573
BsaBI GATNNNNATC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 416
BsaXI ACNNNNNCTCC 2 cut(s) 361, 391
Bse1I ACTGG 1 cut(s) 83
Bse3DI GCAATG 1 cut(s) 552
Bse8I GATNNNNATC 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 416
BseGI GGATG 5 cut(s) 509, 618, 689, 706, 710
BseJI GATNNNNATC 1 cut(s) 93
BseMI GCAATG 1 cut(s) 552
BseNI ACTGG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 335
BshFI GGCC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 4 cut(s) 35, 200, 441, 555
BsmFI GGGAC 4 cut(s) 35, 200, 441, 555
BsmI GAATGC 2 cut(s) 61, 242
BsnI GGCC 1 cut(s) 9
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 335
Bsp1407I TGTACA 1 cut(s) 637
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 1 cut(s) 518
BspANI GGCC 1 cut(s) 9
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 3 cut(s) 96, 429, 571
BspPI GGATC 2 cut(s) 89, 102
BsrBI CCGCTC 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 552
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 416
BssMI GATC 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 416
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 668
BstAUI TGTACA 1 cut(s) 637
BstC8I GCNNGC 1 cut(s) 507
BstDEI CTNAG 1 cut(s) 11
BstF5I GGATG 5 cut(s) 509, 618, 689, 706, 710
BstKTI GATC 1 cut(s) 97
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 571
BstSLI GKGCMC 1 cut(s) 335
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuI GTATCC 1 cut(s) 568
BsuRI GGCC 1 cut(s) 9
BtsCI GGATG 5 cut(s) 509, 618, 689, 706, 710
Cac8I GCNNGC 1 cut(s) 507
CciI TCATGA 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 428, 569
Csp6I GTAC 1 cut(s) 638
CviAII CATG 2 cut(s) 284, 596
CviJI RGCY 6 cut(s) 9, 278, 458, 576, 594, 668
CviKI_1 RGCY 6 cut(s) 9, 278, 458, 576, 594, 668
CviQI GTAC 1 cut(s) 638
DdeI CTNAG 1 cut(s) 11
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 668
EarI CTCTTC 1 cut(s) 668
Eco130I CCWWGG 1 cut(s) 416
Eco147I AGGCCT 1 cut(s) 9
Eco47I GGWCC 2 cut(s) 428, 569
Eco88I CYCGRG 1 cut(s) 513
EcoT14I CCWWGG 1 cut(s) 416
EcoT22I ATGCAT 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 2 cut(s) 287, 599
FaqI GGGAC 4 cut(s) 35, 200, 441, 555
FatI CATG 2 cut(s) 283, 595
FauNDI CATATG 1 cut(s) 349
Fnu4HI GCNGC 1 cut(s) 519
FokI GGATG 5 cut(s) 496, 625, 696, 713, 717
Fsp4HI GCNGC 1 cut(s) 519
FspBI CTAG 4 cut(s) 5, 156, 279, 417
GluI GCNGC 1 cut(s) 519
HaeIII GGCC 1 cut(s) 9
HapII CCGG 1 cut(s) 573
Hin1II CATG 2 cut(s) 287, 599
HinfI GANTC 2 cut(s) 159, 401
HpaII CCGG 1 cut(s) 573
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 454, 700
Hpy188III TCNNGA 5 cut(s) 20, 156, 189, 284, 513
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 2 cut(s) 223, 495
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 4 cut(s) 61, 141, 545, 687
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 470
Hsp92II CATG 2 cut(s) 287, 599
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 589
LpnPI CCDG 2 cut(s) 64, 586
LweI GCATC 4 cut(s) 518, 571, 603, 674
MaeI CTAG 4 cut(s) 5, 156, 279, 417
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 2 cut(s) 128, 445
MalI GATC 1 cut(s) 96
MbiI CCGCTC 1 cut(s) 518
MboI GATC 1 cut(s) 94
MboII GAAGA 4 cut(s) 245, 320, 635, 685
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 4 cut(s) 37, 150, 551, 562
MmeI TCCRAC 1 cut(s) 678
MnlI CCTC 3 cut(s) 185, 264, 487
Mph1103I ATGCAT 1 cut(s) 63
MseI TTAA 3 cut(s) 51, 540, 724
MslI CAYNNNNRTG 2 cut(s) 174, 692
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 1 cut(s) 573
Mva1269I GAATGC 2 cut(s) 61, 242
MwoI GCNNNNNNNGC 1 cut(s) 515
NciI CCSGG 1 cut(s) 573
NdeI CATATG 1 cut(s) 349
NdeII GATC 1 cut(s) 94
NlaIII CATG 2 cut(s) 287, 599
NlaIV GGNNCC 3 cut(s) 96, 429, 571
NsiI ATGCAT 1 cut(s) 63
OliI CACNNNNGTG 1 cut(s) 174
PagI TCATGA 1 cut(s) 283
PceI AGGCCT 1 cut(s) 9
PctI GAATGC 2 cut(s) 61, 242
PfeI GAWTC 2 cut(s) 159, 401
PkrI GCNGC 1 cut(s) 520
PspN4I GGNNCC 3 cut(s) 96, 429, 571
PspPI GGNCC 2 cut(s) 428, 569
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 1 cut(s) 639
RsaNI GTAC 1 cut(s) 638
RseI CAYNNNNRTG 2 cut(s) 174, 692
SaqAI TTAA 3 cut(s) 51, 540, 724
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 1 cut(s) 94
Sau96I GGNCC 2 cut(s) 428, 569
ScrFI CCNGG 1 cut(s) 573
SduI GDGCHC 1 cut(s) 335
SetI ASST 8 cut(s) 280, 418, 422, 443, 460, 473, 484, 721
SfaNI GCATC 4 cut(s) 518, 571, 603, 674
SinI GGWCC 2 cut(s) 428, 569
SmiMI CAYNNNNRTG 2 cut(s) 174, 692
Sse9I AATT 4 cut(s) 37, 150, 551, 562
SseBI AGGCCT 1 cut(s) 9
SsiI CCGC 1 cut(s) 518
SspI AATATT 1 cut(s) 533
SspMI CTAG 4 cut(s) 5, 156, 279, 417
StuI AGGCCT 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 571
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 473
TaqI TCGA 1 cut(s) 710
TasI AATT 4 cut(s) 37, 150, 551, 562
TatI WGTACW 1 cut(s) 637
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 2 cut(s) 159, 401
Tru1I TTAA 3 cut(s) 51, 540, 724
Tru9I TTAA 3 cut(s) 51, 540, 724
TspDTI ATGAA 6 cut(s) 37, 54, 543, 563, 612, 665
VpaK11BI GGWCC 2 cut(s) 428, 569
XbaI TCTAGA 1 cut(s) 155
XmaJI CCTAGG 1 cut(s) 416
XspI CTAG 4 cut(s) 5, 156, 279, 417
Zsp2I ATGCAT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.