Rroxscaffold_7G00211460

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016421
Physical Location & Seq
Forward (+)
61900356 .. 61902893
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00211460.1

Sequence Viewer

Length: 654 bp
ATGCCTTCCGGACTCTGTGGGTCCTCCGAGAAGTACCAGCCGCCACCGAAAATAATTCGAGATTCGGAGATACCGCAGCCTGGTCTATATGGGAGTTTAAGATCCGGGCCGGTATCAGCCATGAGTGAGAAAGGGGAAGATGGAAGAGACAAAGCATGGAAGTACACAAAAAAATTGGAAGGGGAGAGATATTTTAGATGTGTATTTTGTAATCAAGTATGCGGTGGGACTCTATCTAGACTTAAGCATCATTTAGCAGGTGTTGGGGGTTGTATGAAAGCATGTAATAAAGTCCCTGGAGATGTGAAGAAAGAGTACGAATTACTTTTAAAAGTATTTAAAGATGATAAAAAGAAGAGAAGTATTATGTTGCGTGAGATTGGGGCAGGAATTGGTGGAGGAAGTAATAGTTTTGATTCTCCATTAAGTGAAGAATGTGGGAGTAACCTAAGTAGTACTACAGTTCAACCAATGTTGCGTAAGGTAAGATCCAAGAAGAAAATGAGAAAACTTGCTGCATTACTTAGGGATGAAGATCATGAGATAGAAGACCTTACTTTGCCTCCCCACACTGATGCAACATTGGACATTGATGATTGTGATGATATTCATGTGGGTGTGGATGGATATGATGATTATGAATTGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

217

Amino Acids

24.03

Weight (kDa)

6.25

Isoelectric Point (pI)

55.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 8
AciI CCGC 3 cut(s) 41, 74, 222
AclWI GGATC 2 cut(s) 96, 483
AfaI GTAC 4 cut(s) 35, 164, 317, 457
AfiI CCNNNNNNNGG 1 cut(s) 80
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 1 cut(s) 467
AjnI CCWGG 2 cut(s) 79, 295
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 2 cut(s) 96, 483
Aor13HI TCCGGA 1 cut(s) 8
AoxI GGCC 1 cut(s) 107
ApeKI GCWGC 2 cut(s) 76, 515
AspS9I GGNCC 2 cut(s) 21, 107
AsuC2I CCSGG 1 cut(s) 106
AvaII GGWCC 1 cut(s) 21
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BbsI GAAGAC 1 cut(s) 555
BbvI GCAGC 2 cut(s) 88, 502
BccI CCATC 2 cut(s) 134, 617
BciT130I CCWGG 2 cut(s) 81, 297
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 237
BfmI CTRYAG 1 cut(s) 459
BfrI CTTAAG 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 3 cut(s) 41, 77, 516
BlsI GCNGC 3 cut(s) 42, 78, 517
BmcAI AGTACT 1 cut(s) 457
Bme1390I CCNGG 3 cut(s) 81, 106, 297
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 2 cut(s) 21, 107
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 3 cut(s) 81, 106, 297
BmsI GCATC 2 cut(s) 256, 565
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 106
BsaBI GATNNNNATC 1 cut(s) 534
BsaJI CCNNGG 1 cut(s) 295
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 547, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 534
BseAI TCCGGA 1 cut(s) 8
BseBI CCWGG 2 cut(s) 81, 297
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 2 cut(s) 535, 628
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 1 cut(s) 80
BseXI GCAGC 2 cut(s) 88, 502
BshFI GGCC 1 cut(s) 109
BsiSI CCGG 3 cut(s) 9, 105, 110
BslFI GGGAC 2 cut(s) 241, 278
BslI CCNNNNNNNGG 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 2 cut(s) 241, 278
BsnI GGCC 1 cut(s) 109
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 3 cut(s) 101, 488, 535
BspACI CCGC 3 cut(s) 41, 74, 222
BspANI GGCC 1 cut(s) 109
BspEI TCCGGA 1 cut(s) 8
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 96, 483
BspTI CTTAAG 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 3 cut(s) 101, 488, 535
Bst2UI CCWGG 2 cut(s) 81, 297
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 2 cut(s) 139, 350
BstAFI CTTAAG 1 cut(s) 242
BstDEI CTNAG 2 cut(s) 449, 524
BstF5I GGATG 2 cut(s) 535, 628
BstKTI GATC 3 cut(s) 104, 491, 538
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 3 cut(s) 101, 488, 535
BstNI CCWGG 2 cut(s) 81, 297
BstNSI RCATGY 1 cut(s) 285
BstSCI CCNGG 3 cut(s) 79, 104, 295
BstSFI CTRYAG 1 cut(s) 459
BstV1I GCAGC 2 cut(s) 88, 502
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 2 cut(s) 101, 488
BstYI RGATCY 2 cut(s) 101, 488
BsuRI GGCC 1 cut(s) 109
BtsCI GGATG 2 cut(s) 535, 628
BtsIMutI CAGTG 1 cut(s) 570
BveI ACCTGC 1 cut(s) 248
CciI TCATGA 1 cut(s) 538
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 21, 107
Csp6I GTAC 4 cut(s) 34, 163, 316, 456
CviAII CATG 5 cut(s) 121, 156, 282, 539, 611
CviJI RGCY 4 cut(s) 40, 79, 109, 119
CviKI_1 RGCY 4 cut(s) 40, 79, 109, 119
CviQI GTAC 4 cut(s) 34, 163, 316, 456
DdeI CTNAG 2 cut(s) 449, 524
DpnI GATC 3 cut(s) 103, 490, 537
DpnII GATC 3 cut(s) 101, 488, 535
DraI TTTAAA 2 cut(s) 330, 340
Eam1104I CTCTTC 2 cut(s) 139, 350
EarI CTCTTC 2 cut(s) 139, 350
Eco47I GGWCC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 21
EcoRII CCWGG 2 cut(s) 79, 295
FaeI CATG 5 cut(s) 124, 159, 285, 542, 614
FaqI GGGAC 2 cut(s) 241, 278
FatI CATG 5 cut(s) 120, 155, 281, 538, 610
Fnu4HI GCNGC 3 cut(s) 41, 77, 516
FokI GGATG 2 cut(s) 542, 635
Fsp4HI GCNGC 3 cut(s) 41, 77, 516
FspBI CTAG 1 cut(s) 237
GluI GCNGC 3 cut(s) 41, 77, 516
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 1 cut(s) 109
HapII CCGG 3 cut(s) 9, 105, 110
Hin1II CATG 5 cut(s) 124, 159, 285, 542, 614
HinfI GANTC 4 cut(s) 12, 62, 229, 416
HpaII CCGG 3 cut(s) 9, 105, 110
Hpy166II GTNNAC 1 cut(s) 165
Hpy188I TCNGA 2 cut(s) 28, 67
Hpy188III TCNNGA 4 cut(s) 9, 59, 237, 539
Hpy8I GTNNAC 1 cut(s) 165
HpyAV CCTTC 2 cut(s) 15, 173
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 2 cut(s) 518, 578
HpyF3I CTNAG 2 cut(s) 449, 524
Hsp92II CATG 5 cut(s) 124, 159, 285, 542, 614
Kpn2I TCCGGA 1 cut(s) 8
Kzo9I GATC 3 cut(s) 101, 488, 535
Lsp1109I GCAGC 2 cut(s) 88, 502
LweI GCATC 2 cut(s) 256, 565
MaeI CTAG 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 443
MalI GATC 3 cut(s) 103, 490, 537
MboI GATC 3 cut(s) 101, 488, 535
MboII GAAGA 8 cut(s) 149, 156, 319, 367, 443, 508, 545, 560
MflI RGATCY 2 cut(s) 101, 488
MluCI AATT 5 cut(s) 54, 173, 320, 390, 641
MlyI GAGTC 2 cut(s) 6, 223
MnlI CCTC 3 cut(s) 34, 392, 573
MroI TCCGGA 1 cut(s) 8
MseI TTAA 5 cut(s) 98, 243, 329, 339, 425
MslI CAYNNNNRTG 2 cut(s) 573, 615
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 3 cut(s) 9, 105, 110
MspR9I CCNGG 3 cut(s) 81, 106, 297
MvaI CCWGG 2 cut(s) 81, 297
NciI CCSGG 1 cut(s) 106
NdeII GATC 3 cut(s) 101, 488, 535
NlaIII CATG 5 cut(s) 124, 159, 285, 542, 614
NlaIV GGNNCC 1 cut(s) 22
NspI RCATGY 1 cut(s) 285
PagI TCATGA 1 cut(s) 538
PaqCI CACCTGC 1 cut(s) 248
PfeI GAWTC 2 cut(s) 62, 416
PkrI GCNGC 3 cut(s) 42, 78, 517
PleI GAGTC 2 cut(s) 6, 223
PpsI GAGTC 2 cut(s) 6, 223
PpuMI RGGWCCY 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 79, 295
PspGI CCWGG 2 cut(s) 79, 295
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 21, 107
PspPPI RGGWCCY 1 cut(s) 21
PsuI RGATCY 2 cut(s) 101, 488
RsaI GTAC 4 cut(s) 35, 164, 317, 457
RsaNI GTAC 4 cut(s) 34, 163, 316, 456
RseI CAYNNNNRTG 2 cut(s) 573, 615
SaqAI TTAA 5 cut(s) 98, 243, 329, 339, 425
SatI GCNGC 3 cut(s) 41, 77, 516
Sau3AI GATC 3 cut(s) 101, 488, 535
Sau96I GGNCC 2 cut(s) 21, 107
ScaI AGTACT 1 cut(s) 457
SchI GAGTC 2 cut(s) 6, 223
ScrFI CCNGG 3 cut(s) 81, 106, 297
SetI ASST 4 cut(s) 262, 450, 486, 555
SfaNI GCATC 2 cut(s) 256, 565
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 2 cut(s) 573, 615
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 5 cut(s) 54, 173, 320, 390, 641
SsiI CCGC 3 cut(s) 41, 74, 222
SspMI CTAG 1 cut(s) 237
StyD4I CCNGG 3 cut(s) 79, 104, 295
TaaI ACNGT 1 cut(s) 463
TaqI TCGA 1 cut(s) 58
TasI AATT 5 cut(s) 54, 173, 320, 390, 641
TatI WGTACW 2 cut(s) 162, 455
TauI GCSGC 1 cut(s) 43
TfiI GAWTC 2 cut(s) 62, 416
Tru1I TTAA 5 cut(s) 98, 243, 329, 339, 425
Tru9I TTAA 5 cut(s) 98, 243, 329, 339, 425
TscAI CASTG 1 cut(s) 577
TseI GCWGC 2 cut(s) 76, 515
TspDTI ATGAA 4 cut(s) 290, 546, 599, 654
TspRI CASTG 1 cut(s) 577
Vha464I CTTAAG 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 21
XbaI TCTAGA 1 cut(s) 236
XceI RCATGY 1 cut(s) 285
XspI CTAG 1 cut(s) 237
ZrmI AGTACT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.