pycom12g17300

G-type lectin S-receptor-like serine threonine-protein kinase SD2-5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013054
Physical Location & Seq
Forward (+)
19641301 .. 19643154
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1413 bp
ATGCAGCTGTACTTCTTCATCTCCCTCACCATTTTCTGCACTTACGGCTACTCGAAAACCGATGCTCCTCTCGGATACCAATTCAGGTTGCCAGTGCCATTTGAGAACAGTTTGGGATCCAGAGCTTTTTTGATGGAGAACAATGAAACCAAACCAAGTTTTAAAGTTGGAATGAGTGTGGAACCTGTGAGTGGGAAGTACTCATGCTCCTTGCAAGTCTTTCTTGGAGATGTCAAGGTGTGGAGTTCTGGCCATTACTCAAAGTTTTATACTTCAAAAATATGTGTTCTTGAGCTCTCCCGTGACGGTGATTTGAGATTGAAGGGCCCAAAAGGTAGAGTGGGATGGAGAAGTGGGACTTCTGGACAAGGTGTGCAGAAATTACAGATATTGAGGACAGGCAATCTAGTTCTAGTTGATGCATTAGGCAGAATAAAGTGGCAGAGTTTTAATTTTCCAACTGATGTAATGCTTTGGGGACAGAGACTAAGCGTGGCTTCTAGGTTGACTTCATTCCCAAGCAACTCCACTTCATACTATTCTTTCGAAATTGAACCCAGCAGGATTGCTCTCTACTTGAATTCTGGTAAGTGGAACTATTCCTATTGGGAATTCAAGCCCACCGAGAACAGAAACATTGCTTATATTCAATTGGGTCCAAAAGGGTTAGAGTTATTCAGTTATAATCAAAAGAAAATTGCACAGATTCATCCATCTGATGAAAAATTTCAGTTTCAGCCCATGAGGTTTTTAGCATTGGGGAACCAAACAGGAAATTTGAGGCTCTATTTTTACTCACCTAGTATGTCGAAATTTGATGCTTCTTTTCAAGCGCTCAACGCCACATGTGATCTTCCATTGGCGTGTAAGCCCTACGGAATTTGTACATTGTCCGGAGCTTGTTCATGCATTCAACTCATGCCCACCGAAAATGAGACAGGTACTAGTACTAGTACTACTGCTTCTGACTGCGGACGAGGAATTTCATCAGGAGGGTTTTGCAAAGGAGGGAAGAAAACGAAGGTGGAGATGCGTGAATTGAAGGGTGTTACTAGTGTTTTGAGAGGAGGTGCTAAGATTGCTAATGTGAGCAAAGAAGAATGTGGTAATTTGTGCTTCGAGGACTGTAATTGTACAGCAGCATTGTACTCTTCTGCAAAAGAGTGCTTTATATATGGAATGGTAATTGGTGTTAAACAGGTTGAGAATAAGGGAAGTGGATTATTGAGTTATATGGTAAAGGTTCCAAGGGGAGGTCATGGGGGTCATGGGAAGTCAAATTTGAAGAAATGGGTTTTGATCTTGGTAGGAGTGGTTGATGGGTTGATTATTCTTCTTGTTTTTGGAGGGCTTGGATATTACTTGGTAAGGAAGAGAAGAAGACACTCATTGCCTAATGGCCATAGCAACTGA

Protein Analysis

471

Amino Acids

52.04

Weight (kDa)

9.43

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 92 - 167 5.4e-10 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51710
fragaria_vesca FvH4_6g10470
malus_domestica MD04G1170300.v1.1 MD12G1184300.v1.1
prunus_persica Prupe.4G286300_v2.0.a1 Prupe.6G289100_v2.0.a1
pyrus_communis pycom12g17300
rosa_chinensis RchiOBHm_Chr3g0461371
rosa_laevigata RLG00000024937
rosa_multiflora Rmu_sc0002677.1_g000023
rosa_roxburghii Rroxscaffold_6G00418360 Rroxscaffold_6G00418660
rosa_rugosa Rorug03G0049700
rosa_samantha Rh3AG107800 Rh3BG111800 Rh3CG113600 Rh3DG113600
rosa_wichuraiana Rw3G009200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AccIII TCCGGA 1 cut(s) 893
AciI CCGC 1 cut(s) 972
AclWI GGATC 2 cut(s) 111, 124
AcoI YGGCCR 2 cut(s) 250, 1399
AcsI RAATTY 8 cut(s) 580, 611, 725, 775, 812, 879, 981, 1279
AfaI GTAC 8 cut(s) 11, 200, 886, 943, 949, 955, 1135, 1148
AfeI AGCGCT 1 cut(s) 834
AfiI CCNNNNNNNGG 2 cut(s) 191, 1253
AflIII ACRYGT 1 cut(s) 845
AhlI ACTAGT 3 cut(s) 944, 950, 1052
AluBI AGCT 4 cut(s) 7, 125, 295, 899
AluI AGCT 4 cut(s) 7, 125, 295, 899
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 478, 929
AlwI GGATC 2 cut(s) 111, 124
Aor13HI TCCGGA 1 cut(s) 893
Aor51HI AGCGCT 1 cut(s) 834
AoxI GGCC 3 cut(s) 250, 325, 1399
ApaI GGGCCC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 4, 1139
ApoI RAATTY 8 cut(s) 580, 611, 725, 775, 812, 879, 981, 1279
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 1 cut(s) 835
AspS9I GGNCC 3 cut(s) 325, 326, 656
AsuHPI GGTGA 3 cut(s) 19, 320, 789
AsuII TTCGAA 1 cut(s) 546
AvaII GGWCC 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 329
BalI TGGCCA 2 cut(s) 252, 1401
BamHI GGATCC 1 cut(s) 116
BanII GRGCYC 2 cut(s) 297, 329
BarI GAAGNNNNNNTAC 2 cut(s) 946, 978
BbsI GAAGAC 1 cut(s) 1387
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 16, 1151
BccI CCATC 4 cut(s) 127, 339, 721, 1313
BceAI ACGGC 1 cut(s) 61
BciVI GTATCC 1 cut(s) 68
BcoDI GTCTC 2 cut(s) 478, 929
BcuI ACTAGT 3 cut(s) 944, 950, 1052
BfaI CTAG 7 cut(s) 407, 413, 501, 801, 945, 951, 1053
BfoI RGCGCY 1 cut(s) 836
BfuI GTATCC 1 cut(s) 68
BisI GCNGC 2 cut(s) 5, 1140
BlsI GCNGC 2 cut(s) 6, 1141
BmcAI AGTACT 3 cut(s) 200, 949, 955
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 3 cut(s) 325, 326, 656
BmiI GGNNCC 6 cut(s) 118, 183, 327, 657, 764, 1245
BmsI GCATC 4 cut(s) 52, 409, 808, 1020
BpiI GAAGAC 1 cut(s) 1387
Bpu14I TTCGAA 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 1247
BsaWI WCCGGW 1 cut(s) 893
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 191, 221
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 1253
Bse1I ACTGG 1 cut(s) 92
Bse3DI GCAATG 2 cut(s) 636, 1388
BseAI TCCGGA 1 cut(s) 893
BseDI CCNNGG 1 cut(s) 1247
BseGI GGATG 2 cut(s) 350, 709
BseLI CCNNNNNNNGG 2 cut(s) 191, 1253
BseMI GCAATG 2 cut(s) 636, 1388
BseNI ACTGG 1 cut(s) 92
BseRI GAGGAG 2 cut(s) 57, 1080
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 2 cut(s) 16, 1151
BseYI CCCAGC 1 cut(s) 557
BsgI GTGCAG 2 cut(s) 22, 395
BshFI GGCC 3 cut(s) 252, 327, 1401
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 894
BslFI GGGAC 2 cut(s) 370, 492
BslI CCNNNNNNNGG 2 cut(s) 191, 1253
BsmAI GTCTC 2 cut(s) 478, 929
BsmFI GGGAC 2 cut(s) 370, 492
BsmI GAATGC 1 cut(s) 909
BsnI GGCC 3 cut(s) 252, 327, 1401
Bsp119I TTCGAA 1 cut(s) 546
Bsp120I GGGCCC 1 cut(s) 325
Bsp1286I GDGCHC 2 cut(s) 297, 329
Bsp13I TCCGGA 1 cut(s) 893
Bsp1407I TGTACA 2 cut(s) 884, 1133
Bsp143I GATC 3 cut(s) 116, 850, 1299
BspACI CCGC 1 cut(s) 972
BspANI GGCC 3 cut(s) 252, 327, 1401
BspEI TCCGGA 1 cut(s) 893
BspLI GGNNCC 6 cut(s) 118, 183, 327, 657, 764, 1245
BspPI GGATC 2 cut(s) 111, 124
BspT104I TTCGAA 1 cut(s) 546
BsrDI GCAATG 2 cut(s) 636, 1388
BsrGI TGTACA 2 cut(s) 884, 1133
BsrI ACTGG 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 1247
BssMI GATC 3 cut(s) 116, 850, 1299
BssT1I CCWWGG 1 cut(s) 1247
Bst4CI ACNGT 3 cut(s) 110, 308, 1127
Bst6I CTCTTC 2 cut(s) 1156, 1367
BstAUI TGTACA 2 cut(s) 884, 1133
BstBI TTCGAA 1 cut(s) 546
BstDEI CTNAG 2 cut(s) 488, 1074
BstF5I GGATG 2 cut(s) 350, 709
BstH2I RGCGCY 1 cut(s) 836
BstHHI GCGC 1 cut(s) 835
BstKTI GATC 3 cut(s) 119, 853, 1302
BstMAI GTCTC 2 cut(s) 478, 929
BstMBI GATC 3 cut(s) 116, 850, 1299
BstMWI GCNNNNNNNGC 3 cut(s) 45, 839, 1079
BstNSI RCATGY 1 cut(s) 849
BstSLI GKGCMC 1 cut(s) 329
BstV1I GCAGC 2 cut(s) 16, 1151
BstV2I GAAGAC 1 cut(s) 1387
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BsuI GTATCC 1 cut(s) 68
BsuRI GGCC 3 cut(s) 252, 327, 1401
BtsCI GGATG 2 cut(s) 350, 709
BtsIMutI CAGTG 1 cut(s) 99
CfoI GCGC 1 cut(s) 835
Cfr13I GGNCC 3 cut(s) 325, 326, 656
Csp6I GTAC 8 cut(s) 10, 199, 885, 942, 948, 954, 1134, 1147
CviAII CATG 7 cut(s) 204, 742, 846, 906, 919, 1259, 1268
CviQI GTAC 8 cut(s) 10, 199, 885, 942, 948, 954, 1134, 1147
DdeI CTNAG 2 cut(s) 488, 1074
DpnI GATC 3 cut(s) 118, 852, 1301
DpnII GATC 3 cut(s) 116, 850, 1299
DraI TTTAAA 1 cut(s) 163
EaeI YGGCCR 2 cut(s) 250, 1399
Eam1104I CTCTTC 2 cut(s) 1156, 1367
EarI CTCTTC 2 cut(s) 1156, 1367
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 1247
Eco24I GRGCYC 2 cut(s) 297, 329
Eco47I GGWCC 1 cut(s) 656
Eco47III AGCGCT 1 cut(s) 834
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 325
EcoRI GAATTC 2 cut(s) 580, 611
EcoT14I CCWWGG 1 cut(s) 1247
EcoT22I ATGCAT 2 cut(s) 424, 911
EcoT38I GRGCYC 2 cut(s) 297, 329
ErhI CCWWGG 1 cut(s) 1247
FaeI CATG 7 cut(s) 207, 745, 849, 909, 922, 1262, 1271
FalI AAGNNNNNCTT 6 cut(s) 207, 239, 343, 375, 481, 513
FaqI GGGAC 2 cut(s) 370, 492
FatI CATG 7 cut(s) 203, 741, 845, 905, 918, 1258, 1267
Fnu4HI GCNGC 2 cut(s) 5, 1140
FokI GGATG 2 cut(s) 357, 696
FriOI GRGCYC 2 cut(s) 297, 329
Fsp4HI GCNGC 2 cut(s) 5, 1140
FspBI CTAG 7 cut(s) 407, 413, 501, 801, 945, 951, 1053
GlaI GCGC 1 cut(s) 834
GluI GCNGC 2 cut(s) 5, 1140
GsaI CCCAGC 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 836
HaeIII GGCC 3 cut(s) 252, 327, 1401
HapII CCGG 1 cut(s) 894
HhaI GCGC 1 cut(s) 835
Hin1II CATG 7 cut(s) 207, 745, 849, 909, 922, 1262, 1271
Hin6I GCGC 1 cut(s) 833
HinP1I GCGC 1 cut(s) 833
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HinfI GANTC 1 cut(s) 706
HpaII CCGG 1 cut(s) 894
HphI GGTGA 3 cut(s) 19, 320, 789
Hpy166II GTNNAC 1 cut(s) 507
Hpy188I TCNGA 3 cut(s) 74, 718, 967
Hpy188III TCNNGA 5 cut(s) 120, 290, 363, 894, 990
Hpy8I GTNNAC 1 cut(s) 507
HpyAV CCTTC 3 cut(s) 316, 1015, 1036
HpyCH4III ACNGT 3 cut(s) 110, 308, 1127
HpyCH4V TGCA 9 cut(s) 4, 39, 214, 376, 422, 701, 909, 1002, 1157
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 839, 1079
HpyF3I CTNAG 2 cut(s) 488, 1074
Hsp92II CATG 7 cut(s) 207, 745, 849, 909, 922, 1262, 1271
HspAI GCGC 1 cut(s) 833
Kpn2I TCCGGA 1 cut(s) 893
Kzo9I GATC 3 cut(s) 116, 850, 1299
LmnI GCTCC 3 cut(s) 70, 212, 896
Lsp1109I GCAGC 2 cut(s) 16, 1151
LweI GCATC 4 cut(s) 52, 409, 808, 1020
MaeI CTAG 7 cut(s) 407, 413, 501, 801, 945, 951, 1053
MaeIII GTNAC 2 cut(s) 302, 1048
MalI GATC 3 cut(s) 118, 852, 1301
MboI GATC 3 cut(s) 116, 850, 1299
MfeI CAATTG 1 cut(s) 650
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 2 cut(s) 297, 329
MlsI TGGCCA 2 cut(s) 252, 1401
MluNI TGGCCA 2 cut(s) 252, 1401
MmeI TCCRAC 2 cut(s) 148, 482
Mox20I TGGCCA 2 cut(s) 252, 1401
Mph1103I ATGCAT 2 cut(s) 424, 911
MroI TCCGGA 1 cut(s) 893
MroXI GAANNNNTTC 1 cut(s) 726
MscI TGGCCA 2 cut(s) 252, 1401
MseI TTAA 3 cut(s) 162, 450, 1194
MslI CAYNNNNRTG 1 cut(s) 862
Msp20I TGGCCA 2 cut(s) 252, 1401
MspA1I CMGCKG 1 cut(s) 7
MspI CCGG 1 cut(s) 894
MunI CAATTG 1 cut(s) 650
Mva1269I GAATGC 1 cut(s) 909
MwoI GCNNNNNNNGC 3 cut(s) 45, 839, 1079
NdeII GATC 3 cut(s) 116, 850, 1299
NlaIII CATG 7 cut(s) 207, 745, 849, 909, 922, 1262, 1271
NlaIV GGNNCC 6 cut(s) 118, 183, 327, 657, 764, 1245
NmuCI GTSAC 1 cut(s) 302
NsiI ATGCAT 2 cut(s) 424, 911
NspI RCATGY 1 cut(s) 849
NspV TTCGAA 1 cut(s) 546
PciI ACATGT 1 cut(s) 845
PctI GAATGC 1 cut(s) 909
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 1 cut(s) 706
PkrI GCNGC 2 cut(s) 6, 1141
PscI ACATGT 1 cut(s) 845
PsiI TTATAA 1 cut(s) 684
Psp124BI GAGCTC 1 cut(s) 297
PspFI CCCAGC 1 cut(s) 557
PspN4I GGNNCC 6 cut(s) 118, 183, 327, 657, 764, 1245
PspOMI GGGCCC 1 cut(s) 325
PspPI GGNCC 3 cut(s) 325, 326, 656
PsuI RGATCY 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 7
RsaI GTAC 8 cut(s) 11, 200, 886, 943, 949, 955, 1135, 1148
RsaNI GTAC 8 cut(s) 10, 199, 885, 942, 948, 954, 1134, 1147
RseI CAYNNNNRTG 1 cut(s) 862
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 3 cut(s) 162, 450, 1194
SatI GCNGC 2 cut(s) 5, 1140
Sau3AI GATC 3 cut(s) 116, 850, 1299
Sau96I GGNCC 3 cut(s) 325, 326, 656
ScaI AGTACT 3 cut(s) 200, 949, 955
SduI GDGCHC 2 cut(s) 297, 329
SfaNI GCATC 4 cut(s) 52, 409, 808, 1020
SfuI TTCGAA 1 cut(s) 546
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 862
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SpeI ACTAGT 3 cut(s) 944, 950, 1052
SsiI CCGC 1 cut(s) 972
SspMI CTAG 7 cut(s) 407, 413, 501, 801, 945, 951, 1053
SstI GAGCTC 1 cut(s) 297
StyI CCWWGG 1 cut(s) 1247
TaaI ACNGT 3 cut(s) 110, 308, 1127
TaqI TCGA 4 cut(s) 53, 546, 809, 1119
TatI WGTACW 7 cut(s) 9, 198, 884, 947, 953, 1133, 1146
TfiI GAWTC 1 cut(s) 706
Tru1I TTAA 3 cut(s) 162, 450, 1194
Tru9I TTAA 3 cut(s) 162, 450, 1194
TscAI CASTG 1 cut(s) 99
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 2 cut(s) 4, 1139
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 8 cut(s) 7, 159, 501, 522, 698, 735, 894, 975
TspGWI ACGGA 1 cut(s) 891
TspRI CASTG 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 8 cut(s) 580, 611, 725, 775, 812, 879, 981, 1279
XceI RCATGY 1 cut(s) 849
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 7 cut(s) 407, 413, 501, 801, 945, 951, 1053
ZrmI AGTACT 3 cut(s) 200, 949, 955
Zsp2I ATGCAT 2 cut(s) 424, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.