pycom12g19580

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007938
Physical Location & Seq
Forward (+)
21372575 .. 21373525
951 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19580.1

Sequence Viewer

Length: 471 bp
ATGGACAGAGAAGGAGGATCCAACGGCGGATCTTCCTACTACGCCGTCCTCGGGATCCGCAGGGACGCCTCCTTCTCTGATATCCGCACAGCTTACCGCAAGCTCGCTCTGAAATGGCATCCGGATAGGGCGCGAAGTCAAACGGCGGCGGGAGAAGCCAAGCGGCGGTTTCAGCAAATCCAAGAGGCTTACTCCGTTCTCTCGGATCAGGCGAAGCGGTCGATGTACGACGCCGGGCTCTACGACCCCCTCGAAGAAGAAGACCAGGAATTTTGCAATTTCATGGGTGAAATGCTGTCCATGATGAACAACGTGAAAGACCAGGGGGACAGTTTGGAAGACCTCCAGAGGATGTTCAACGACATGGTCGGCGGAGATGGGATGATGGGATTCGACTTCGGCGACATGGATCCGACGGCGAACAAGAAGCCACGTGTAGGTGCGTCGAGAGGTGCCAAGTCTCGCTGCTAG

Protein Analysis

157

Amino Acids

17.51

Weight (kDa)

5.26

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 12 - 77 1.4e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 452
AccII CGCG 1 cut(s) 133
AccIII TCCGGA 1 cut(s) 121
AclWI GGATC 8 cut(s) 12, 25, 37, 49, 62, 213, 404, 417
AcsI RAATTY 1 cut(s) 269
AcvI CACGTG 1 cut(s) 434
AcyI GRCGYC 2 cut(s) 66, 231
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 3 cut(s) 51, 165, 437
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 1 cut(s) 358
AjnI CCWGG 2 cut(s) 264, 321
AluBI AGCT 2 cut(s) 92, 103
AluI AGCT 2 cut(s) 92, 103
Alw26I GTCTC 1 cut(s) 465
AlwI GGATC 8 cut(s) 12, 25, 37, 49, 62, 213, 404, 417
Ama87I CYCGRG 1 cut(s) 50
Aor13HI TCCGGA 1 cut(s) 121
ApeKI GCWGC 1 cut(s) 465
ApoI RAATTY 1 cut(s) 269
AspLEI GCGC 1 cut(s) 133
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 50
BamHI GGATCC 3 cut(s) 17, 54, 409
BanI GGYRCC 1 cut(s) 452
BanII GRGCYC 1 cut(s) 240
BbrPI CACGTG 1 cut(s) 434
BbsI GAAGAC 2 cut(s) 267, 345
BbvI GCAGC 1 cut(s) 452
BccI CCATC 2 cut(s) 371, 379
BceAI ACGGC 4 cut(s) 29, 40, 159, 432
BciT130I CCWGG 2 cut(s) 266, 323
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 465
BfaI CTAG 1 cut(s) 469
BisI GCNGC 3 cut(s) 147, 164, 466
BlsI GCNGC 3 cut(s) 148, 165, 467
Bme1390I CCNGG 3 cut(s) 235, 266, 323
BmeT110I CYCGRG 1 cut(s) 50
BmiI GGNNCC 4 cut(s) 19, 56, 411, 454
BmrFI CCNGG 3 cut(s) 235, 266, 323
BmsI GCATC 1 cut(s) 127
BpiI GAAGAC 2 cut(s) 267, 345
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpmI CTGGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 434
BsaHI GRCGYC 2 cut(s) 66, 231
BsaJI CCNNGG 2 cut(s) 49, 322
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 165, 437
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 2 cut(s) 266, 323
BseDI CCNNGG 2 cut(s) 49, 322
BseGI GGATG 3 cut(s) 118, 357, 387
BseLI CCNNNNNNNGG 3 cut(s) 51, 165, 437
BseXI GCAGC 1 cut(s) 452
Bsh1236I CGCG 1 cut(s) 133
Bsh1285I CGRYCG 1 cut(s) 221
BshNI GGYRCC 1 cut(s) 452
BsiEI CGRYCG 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 2 cut(s) 122, 234
BslFI GGGAC 2 cut(s) 77, 341
BslI CCNNNNNNNGG 3 cut(s) 51, 165, 437
BsmAI GTCTC 1 cut(s) 465
BsmFI GGGAC 2 cut(s) 77, 341
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 240
Bsp13I TCCGGA 1 cut(s) 121
Bsp143I GATC 5 cut(s) 17, 29, 54, 205, 409
BspEI TCCGGA 1 cut(s) 121
BspFNI CGCG 1 cut(s) 133
BspLI GGNNCC 4 cut(s) 19, 56, 411, 454
BspPI GGATC 8 cut(s) 12, 25, 37, 49, 62, 213, 404, 417
BspT107I GGYRCC 1 cut(s) 452
BssECI CCNNGG 2 cut(s) 49, 322
BssMI GATC 5 cut(s) 17, 29, 54, 205, 409
BssNI GRCGYC 2 cut(s) 66, 231
Bst2UI CCWGG 2 cut(s) 266, 323
Bst4CI ACNGT 1 cut(s) 332
BstACI GRCGYC 2 cut(s) 66, 231
BstBAI YACGTR 1 cut(s) 434
BstC8I GCNNGC 2 cut(s) 101, 105
BstF5I GGATG 3 cut(s) 118, 357, 387
BstFNI CGCG 1 cut(s) 133
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 5 cut(s) 20, 32, 57, 208, 412
BstMAI GTCTC 1 cut(s) 465
BstMBI GATC 5 cut(s) 17, 29, 54, 205, 409
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 2 cut(s) 155, 172
BstNI CCWGG 2 cut(s) 266, 323
BstSCI CCNGG 3 cut(s) 233, 264, 321
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 452
BstV2I GAAGAC 2 cut(s) 267, 345
BstX2I RGATCY 4 cut(s) 17, 29, 54, 409
BstYI RGATCY 4 cut(s) 17, 29, 54, 409
BtsCI GGATG 3 cut(s) 118, 357, 387
Cac8I GCNNGC 2 cut(s) 101, 105
CfoI GCGC 1 cut(s) 133
CseI GACGC 3 cut(s) 74, 239, 432
Csp6I GTAC 1 cut(s) 226
CviAII CATG 4 cut(s) 283, 301, 364, 406
CviJI RGCY 6 cut(s) 92, 103, 158, 188, 238, 430
CviKI_1 RGCY 6 cut(s) 92, 103, 158, 188, 238, 430
CviQI GTAC 1 cut(s) 226
DpnI GATC 5 cut(s) 19, 31, 56, 207, 411
DpnII GATC 5 cut(s) 17, 29, 54, 205, 409
EciI GGCGGA 2 cut(s) 42, 387
Eco24I GRGCYC 1 cut(s) 240
Eco32I GATATC 1 cut(s) 82
Eco72I CACGTG 1 cut(s) 434
Eco88I CYCGRG 1 cut(s) 50
EcoRII CCWGG 2 cut(s) 264, 321
EcoRV GATATC 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 4 cut(s) 286, 304, 367, 409
FaiI YATR 4 cut(s) 284, 302, 365, 407
FaqI GGGAC 2 cut(s) 77, 341
FatI CATG 4 cut(s) 282, 300, 363, 405
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 3 cut(s) 147, 164, 466
FokI GGATG 3 cut(s) 105, 364, 394
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 3 cut(s) 147, 164, 466
FspBI CTAG 1 cut(s) 469
GlaI GCGC 1 cut(s) 132
GluI GCNGC 3 cut(s) 147, 164, 466
GsuI CTGGAG 1 cut(s) 329
HapII CCGG 2 cut(s) 122, 234
HgaI GACGC 3 cut(s) 74, 239, 432
HhaI GCGC 1 cut(s) 133
Hin1I GRCGYC 2 cut(s) 66, 231
Hin1II CATG 4 cut(s) 286, 304, 367, 409
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HinfI GANTC 1 cut(s) 390
HpaII CCGG 2 cut(s) 122, 234
HphI GGTGA 1 cut(s) 299
Hpy188I TCNGA 4 cut(s) 79, 111, 205, 414
Hpy188III TCNNGA 4 cut(s) 52, 122, 346, 447
Hpy99I CGWCG 3 cut(s) 233, 418, 448
HpyAV CCTTC 2 cut(s) 5, 82
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 2 cut(s) 312, 433
HpyCH4V TGCA 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 172
HpySE526I ACGT 2 cut(s) 312, 433
Hsp92I GRCGYC 2 cut(s) 66, 231
Hsp92II CATG 4 cut(s) 286, 304, 367, 409
HspAI GCGC 1 cut(s) 131
Kpn2I TCCGGA 1 cut(s) 121
Kzo9I GATC 5 cut(s) 17, 29, 54, 205, 409
LpnPI CCDG 9 cut(s) 46, 135, 194, 247, 251, 278, 308, 335, 359
Lsp1109I GCAGC 1 cut(s) 452
LweI GCATC 1 cut(s) 127
MaeI CTAG 1 cut(s) 469
MaeII ACGT 2 cut(s) 312, 433
MalI GATC 5 cut(s) 19, 31, 56, 207, 411
MboI GATC 5 cut(s) 17, 29, 54, 205, 409
MboII GAAGA 5 cut(s) 24, 266, 269, 272, 350
MflI RGATCY 4 cut(s) 17, 29, 54, 409
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 2 cut(s) 269, 277
MmeI TCCRAC 2 cut(s) 45, 437
MnlI CCTC 8 cut(s) 8, 59, 79, 178, 260, 342, 353, 443
MroI TCCGGA 1 cut(s) 121
MspI CCGG 2 cut(s) 122, 234
MspR9I CCNGG 3 cut(s) 235, 266, 323
MvaI CCWGG 2 cut(s) 266, 323
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 2 cut(s) 155, 172
NciI CCSGG 1 cut(s) 235
NdeII GATC 5 cut(s) 17, 29, 54, 205, 409
NlaIII CATG 4 cut(s) 286, 304, 367, 409
NlaIV GGNNCC 4 cut(s) 19, 56, 411, 454
PcsI WCGNNNNNNNCGW 3 cut(s) 209, 249, 399
PfeI GAWTC 1 cut(s) 390
PflFI GACNNNGTC 1 cut(s) 365
PkrI GCNGC 3 cut(s) 148, 165, 467
PmaCI CACGTG 1 cut(s) 434
PmlI CACGTG 1 cut(s) 434
Ppu21I YACGTR 1 cut(s) 434
Psp6I CCWGG 2 cut(s) 264, 321
PspCI CACGTG 1 cut(s) 434
PspGI CCWGG 2 cut(s) 264, 321
PspN4I GGNNCC 4 cut(s) 19, 56, 411, 454
PsuI RGATCY 4 cut(s) 17, 29, 54, 409
PsyI GACNNNGTC 1 cut(s) 365
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SatI GCNGC 3 cut(s) 147, 164, 466
Sau3AI GATC 5 cut(s) 17, 29, 54, 205, 409
ScrFI CCNGG 3 cut(s) 235, 266, 323
SduI GDGCHC 1 cut(s) 240
SetI ASST 7 cut(s) 94, 105, 315, 345, 436, 442, 454
SfaNI GCATC 1 cut(s) 127
Sse9I AATT 2 cut(s) 269, 277
SspMI CTAG 1 cut(s) 469
StyD4I CCNGG 3 cut(s) 233, 264, 321
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 2 cut(s) 315, 436
TaqI TCGA 4 cut(s) 221, 252, 393, 446
TasI AATT 2 cut(s) 269, 277
TauI GCSGC 2 cut(s) 149, 166
TfiI GAWTC 1 cut(s) 390
TseI GCWGC 1 cut(s) 465
TspDTI ATGAA 2 cut(s) 271, 320
TspGWI ACGGA 1 cut(s) 184
Tth111I GACNNNGTC 1 cut(s) 365
XapI RAATTY 1 cut(s) 269
XspI CTAG 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.