Rh3DG079500

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0007938
Physical Location & Seq
Reverse (-)
6169194 .. 6170802
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG079500.1

Sequence Viewer

Length: 399 bp
ATGGACAGGGATGGAGGATCCAACGGCGGATCTTGCTACTACTCCGTCCTCGGGATCCGCAAGGATGCCTCCTTCTCTGATATCCGCACCGCTTACCGCAAACTCGCCATGAAATGGCATCCGGATAGGTGGGCCCGGAACCAGAACCCGTCGGTGGCCGGAGAAGCGAATCGGCGGTTTCAGCAAATCCAAGAAGCCTATTCGGTTCTCTCGGATCAGGGGAAGAGGTCAATGTACGACGCCGGGGTCTACGACCCGCTTGAAGAAGAAGACGAAGAATTTGGGGACTTCATGCAAGAGATGGTCTCCATGATGAACAGTGTGAAGGACGAGGTAGATATTTATTCGTTTTACCTATTTATTCCTTATTTGTTCAGGGGTGAAATGGTAATTCCCTAA

Protein Analysis

132

Amino Acids

15.31

Weight (kDa)

4.68

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 13 - 80 1.6e-23 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 114
AccI GTMKAC 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 7 cut(s) 27, 58, 85, 90, 97, 175, 257
AclWI GGATC 6 cut(s) 12, 25, 37, 49, 62, 222
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 3 cut(s) 51, 114, 154
AgsI TTSAA 1 cut(s) 263
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 6 cut(s) 12, 25, 37, 49, 62, 222
Ama87I CYCGRG 1 cut(s) 50
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 2 cut(s) 132, 156
ApaI GGGCCC 1 cut(s) 136
ApoI RAATTY 1 cut(s) 278
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspS9I GGNCC 2 cut(s) 132, 133
AsuC2I CCSGG 2 cut(s) 136, 244
AsuHPI GGTGA 1 cut(s) 392
AvaI CYCGRG 1 cut(s) 50
BaeGI GKGCMC 1 cut(s) 136
BamHI GGATCC 2 cut(s) 17, 54
BanII GRGCYC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 276
BccI CCATC 2 cut(s) 5, 295
BceAI ACGGC 1 cut(s) 40
BcnI CCSGG 2 cut(s) 136, 244
BcoDI GTCTC 1 cut(s) 310
Bme1390I CCNGG 2 cut(s) 136, 244
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 132, 133
BmiI GGNNCC 4 cut(s) 19, 56, 134, 140
BmrFI CCNGG 2 cut(s) 136, 244
BmsI GCATC 2 cut(s) 55, 127
BpiI GAAGAC 1 cut(s) 276
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpuMI CCSGG 2 cut(s) 136, 244
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 49, 243
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 114, 154
BseAI TCCGGA 1 cut(s) 121
BseDI CCNNGG 2 cut(s) 49, 243
BseGI GGATG 3 cut(s) 16, 70, 118
BseLI CCNNNNNNNGG 3 cut(s) 51, 114, 154
BseSI GKGCMC 1 cut(s) 136
BshFI GGCC 2 cut(s) 134, 158
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 4 cut(s) 122, 136, 159, 243
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 3 cut(s) 51, 114, 154
BsmAI GTCTC 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 2 cut(s) 134, 158
Bso31I GGTCTC 1 cut(s) 310
BsoBI CYCGRG 1 cut(s) 50
Bsp120I GGGCCC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 136
Bsp13I TCCGGA 1 cut(s) 121
Bsp143I GATC 4 cut(s) 17, 29, 54, 214
BspACI CCGC 7 cut(s) 27, 58, 85, 90, 97, 175, 257
BspANI GGCC 2 cut(s) 134, 158
BspEI TCCGGA 1 cut(s) 121
BspLI GGNNCC 4 cut(s) 19, 56, 134, 140
BspPI GGATC 6 cut(s) 12, 25, 37, 49, 62, 222
BspTNI GGTCTC 1 cut(s) 310
BssECI CCNNGG 2 cut(s) 49, 243
BssMI GATC 4 cut(s) 17, 29, 54, 214
BssNI GRCGYC 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 1 cut(s) 218
BstACI GRCGYC 1 cut(s) 240
BstF5I GGATG 3 cut(s) 16, 70, 118
BstKTI GATC 4 cut(s) 20, 32, 57, 217
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 4 cut(s) 17, 29, 54, 214
BstMWI GCNNNNNNNGC 3 cut(s) 33, 164, 181
BstSCI CCNGG 2 cut(s) 134, 242
BstSLI GKGCMC 1 cut(s) 136
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 3 cut(s) 17, 29, 54
BstYI RGATCY 3 cut(s) 17, 29, 54
BsuRI GGCC 2 cut(s) 134, 158
BtsCI GGATG 3 cut(s) 16, 70, 118
BtsIMutI CAGTG 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 132, 133
CseI GACGC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 235
CviAII CATG 3 cut(s) 109, 292, 310
CviJI RGCY 3 cut(s) 134, 158, 197
CviKI_1 RGCY 3 cut(s) 134, 158, 197
CviQI GTAC 1 cut(s) 235
DpnI GATC 4 cut(s) 19, 31, 56, 216
DpnII GATC 4 cut(s) 17, 29, 54, 214
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
EciI GGCGGA 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 310
Eco32I GATATC 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 50
EcoRV GATATC 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 136
FaeI CATG 3 cut(s) 112, 295, 313
FaiI YATR 3 cut(s) 110, 293, 311
FaqI GGGAC 1 cut(s) 299
FatI CATG 3 cut(s) 108, 291, 309
FauI CCCGC 1 cut(s) 264
FblI GTMKAC 1 cut(s) 249
FokI GGATG 3 cut(s) 23, 77, 105
FriOI GRGCYC 1 cut(s) 136
HaeIII GGCC 2 cut(s) 134, 158
HapII CCGG 4 cut(s) 122, 136, 159, 243
HgaI GACGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 3 cut(s) 112, 295, 313
HinfI GANTC 1 cut(s) 169
HpaII CCGG 4 cut(s) 122, 136, 159, 243
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 79, 214
Hpy188III TCNNGA 2 cut(s) 52, 122
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 2 cut(s) 154, 242
HpyAV CCTTC 2 cut(s) 82, 319
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4V TGCA 1 cut(s) 295
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 164, 181
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 3 cut(s) 112, 295, 313
Kpn2I TCCGGA 1 cut(s) 121
Kzo9I GATC 4 cut(s) 17, 29, 54, 214
LpnPI CCDG 7 cut(s) 135, 149, 155, 172, 203, 256, 361
LweI GCATC 2 cut(s) 55, 127
MalI GATC 4 cut(s) 19, 31, 56, 216
MboI GATC 4 cut(s) 17, 29, 54, 214
MboII GAAGA 5 cut(s) 235, 275, 278, 281, 287
MflI RGATCY 3 cut(s) 17, 29, 54
MhlI GDGCHC 1 cut(s) 136
MluCI AATT 2 cut(s) 278, 390
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 5 cut(s) 8, 59, 79, 219, 325
MroI TCCGGA 1 cut(s) 121
MspI CCGG 4 cut(s) 122, 136, 159, 243
MspR9I CCNGG 2 cut(s) 136, 244
MwoI GCNNNNNNNGC 3 cut(s) 33, 164, 181
NciI CCSGG 2 cut(s) 136, 244
NdeII GATC 4 cut(s) 17, 29, 54, 214
NlaIII CATG 3 cut(s) 112, 295, 313
NlaIV GGNNCC 4 cut(s) 19, 56, 134, 140
PfeI GAWTC 1 cut(s) 169
PflMI CCANNNNNTGG 1 cut(s) 114
PspN4I GGNNCC 4 cut(s) 19, 56, 134, 140
PspOMI GGGCCC 1 cut(s) 132
PspPI GGNCC 2 cut(s) 132, 133
PsuI RGATCY 3 cut(s) 17, 29, 54
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
Sau3AI GATC 4 cut(s) 17, 29, 54, 214
Sau96I GGNCC 2 cut(s) 132, 133
ScrFI CCNGG 2 cut(s) 136, 244
SduI GDGCHC 1 cut(s) 136
SetI ASST 4 cut(s) 131, 230, 336, 357
SfaNI GCATC 2 cut(s) 55, 127
Sse9I AATT 2 cut(s) 278, 390
SsiI CCGC 7 cut(s) 27, 58, 85, 90, 97, 175, 257
StyD4I CCNGG 2 cut(s) 134, 242
TaaI ACNGT 1 cut(s) 320
TasI AATT 2 cut(s) 278, 390
TfiI GAWTC 1 cut(s) 169
TscAI CASTG 1 cut(s) 325
TspDTI ATGAA 3 cut(s) 125, 280, 329
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 1 cut(s) 325
Van91I CCANNNNNTGG 1 cut(s) 114
XapI RAATTY 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.