pycom12g20770

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014550
Physical Location & Seq
Forward (+)
22135753 .. 22137453
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1701 bp
ATGAAGAAACACCAGAAAACCATTCTCTCACTCTTGGAAAAATGCAGAAGCATGAGTGAATTGAAACAAATACATGGGCTACGATCGATCACCACCTCAGTTGTTAACAATGTAATCCAGTTAAGTAGGCTTATTGATTTTTGTACCAATTCCGAGGCCAGAGACCTCGGCTACGCGAAGAATGTTTTCCGGCAGATCAGTCAGCCTAGTGTCTACATCTGGAACTCAATGATCAGAGGCTACTCCAATAGTGAGAACCCGGTTGAGTCTTTGATTATGTACAGGGAAATGCTGCAAAGGGGTTACTTGCCCGACAATTTTACTTTCCCGTTCGTGCTTAGATCGTGTTCTGTGATCGCTGATTACCGTTATGGAAAATGTGTTCATAACTGCATTGTGAAAACTGGGTATGGGTTGGAGGTCTATGGCTCTACTTGTTTGCTTAGAATGTATGCTTCCTGCGCAGATATGGAATCTGCACTCAAGGTGTTTGATATTATTCCCAAGTGGAATGTAGTTGCTTGGACTAGTTTGATTTCCGGGTTTGTTAATAACAGTCGGGCTATCGAGGCTATAAAGGTGTTCAAGGATATGGAACTTCACAATGTACAGCCTAATGAGATCACCATGGTGCATGTTGTAGTCGCTTGTGCACGAAGTAGGGATATTGATACAGGAAAGTTGGTTCACAGTCGTATTCGCCAACTAGGGTTTGATCCATTTGGTTCAAGTTTTAACTTTAATGTAGTTCTTGCAACTGCCTTATTAGATATGTACGCAAAATGTGGCAGCTTGAGAACTGCAAGGGATCTGTTCAACAAAATGGCTCAAAAAAACTTGGTTACCTGGAATTCAATGATTAGTGCTTATAATCAGTATGGCCGGGCTGAGGAGGCAATCAATCTCTTTTCTGATATGTGGATTGCTGGTTTTGTTCCAGACAAAACTACCTTTCTAGGTGTAATAGGTGCTTGTGCGCGGTTGGGGGCTTTGGCATTCGGACGAAGTCTTCATTCATATGTATCGAAAACTAGCATGGGCAAAGATACTACAATCGGGACTGCCTTAGTTGACATGTATGCTAAAATTGGAGATGCAGGAAGTGCTCGAAGAATTTTTGAGAACTTGCAGAAGAAAGATGTGATGGCATGGACTAGCATGATTATCTGTTTAGCTACGCATGGACACGGTATGGAAGCGCTACATGCATTTAGAAACATGCAAGAGGATGCTAGTGTTATTCCAGACCAGATCACTTACATTGGGGTTTTATCGGCATGTAGCCATATCGGTCTTGTAGAGGAGGGTCAGAGACATTTTGCCTCAATGGTAAATCTTTACGGCATAAAGCCGACTGTGGATCATTATTGCTGCATGGTTGATCTTCTGAGTCGAGCAGGCCGCTTTGATGAGGCGATGAGACTGCTGGAAGAGATGCCGATCCAACCCAATGTTGCTGTCTGGGGAGCTCTCTTGAACGGCTGCGAGATACATGAAAATGTTGATCTAGCTGACAAAGTGAGAAGATATATAACAGAGATGGAACCTCATGGCAGTGGAGTTTATGTTCTGCTGTCCAATATATACGCTAGGGCTGGTCGATGGCAGGAAGTAAATCGGGTTAGAGAACTGATGAAAAACAGGAATATTGCCAAAAATCTAGGGCATAGTTCGGTCGAAATGGAGTTGTTAAGTTCATAA

Protein Analysis

567

Amino Acids

63.64

Weight (kDa)

8.6

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 69 - 116 1.4e-08 PPR repeat family
PPR_2 PF13041 171 - 211 5.2e-09 PPR repeat family
PPR_2 PF13041 279 - 327 5.8e-10 PPR repeat family
PPR PF01535 281 - 310 4.4e-07 PPR repeat
PPR_1 PF12854 448 - 479 4.6e-07 PPR repeat
E_motif PF20431 498 - 560 1.3e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014550)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05240
fragaria_vesca FvH4_6g05570
malus_domestica MD12G1221500.v1.1
prunus_persica Prupe.6G328300_v2.0.a1
pyrus_communis pycom12g20770
rosa_chinensis RchiOBHm_Chr3g0454351
rosa_laevigata RLG00000025451 RLG00000025452 RLG00000025458
rosa_multiflora Rmu_sc0000006.1_g000021 Rmu_sc0007209.1_g000013
rosa_roxburghii Rroxscaffold_165G00437180 Rroxscaffold_6G00425180
rosa_rugosa Rorug02G0654500
rosa_samantha Rh3BG060200 Rh3DG060600
rosa_wichuraiana Rw3G004500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 870
Acc16I TGCGCA 1 cut(s) 463
AccI GTMKAC 1 cut(s) 213
AccII CGCG 2 cut(s) 176, 979
AciI CCGC 2 cut(s) 979, 1402
AclWI GGATC 4 cut(s) 710, 816, 1368, 1435
AcoI YGGCCR 1 cut(s) 880
AcsI RAATTY 2 cut(s) 850, 1113
AfaI GTAC 4 cut(s) 145, 281, 609, 776
AfeI AGCGCT 1 cut(s) 1200
AfiI CCNNNNNNNGG 1 cut(s) 889
AflIII ACRYGT 1 cut(s) 1074
AgsI TTSAA 6 cut(s) 64, 586, 729, 817, 855, 1477
AhlI ACTAGT 1 cut(s) 527
AjnI CCWGG 1 cut(s) 845
AleI CACNNNNGTG 1 cut(s) 629
AloI GAACNNNNNNTCC 6 cut(s) 366, 398, 669, 701, 1551, 1583
AluBI AGCT 4 cut(s) 792, 1175, 1469, 1511
AluI AGCT 4 cut(s) 792, 1175, 1469, 1511
Alw21I GWGCWC 3 cut(s) 655, 1108, 1471
Alw26I GTCTC 3 cut(s) 156, 1306, 1414
Alw44I GTGCAC 1 cut(s) 651
AlwI GGATC 4 cut(s) 710, 816, 1368, 1435
Aor51HI AGCGCT 1 cut(s) 1200
AoxI GGCC 3 cut(s) 156, 880, 1399
ApaLI GTGCAC 1 cut(s) 651
ApeKI GCWGC 4 cut(s) 292, 789, 1371, 1482
ApoI RAATTY 2 cut(s) 850, 1113
Asp700I GAANNNNTTC 1 cut(s) 185
AspLEI GCGC 3 cut(s) 464, 979, 1201
AsuC2I CCSGG 3 cut(s) 260, 541, 884
AsuHPI GGTGA 2 cut(s) 82, 616
BaeGI GKGCMC 1 cut(s) 655
BanII GRGCYC 1 cut(s) 1471
BbsI GAAGAC 1 cut(s) 1001
Bbv12I GWGCWC 3 cut(s) 655, 1108, 1471
BbvCI CCTCAGC 1 cut(s) 888
BbvI GCAGC 4 cut(s) 279, 801, 1358, 1469
BccI CCATC 3 cut(s) 1138, 1534, 1596
BceAI ACGGC 2 cut(s) 1357, 1495
BcgI CGANNNNNNTGC 2 cut(s) 1405, 1439
BciT130I CCWGG 1 cut(s) 847
BclI TGATCA 1 cut(s) 231
BcnI CCSGG 3 cut(s) 260, 541, 884
BcoDI GTCTC 3 cut(s) 156, 1306, 1414
BcuI ACTAGT 1 cut(s) 527
BfoI RGCGCY 1 cut(s) 1202
BisI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
BlsI GCNGC 5 cut(s) 294, 791, 1373, 1403, 1484
Bme1390I CCNGG 4 cut(s) 260, 541, 847, 884
BmiI GGNNCC 1 cut(s) 1545
BmrFI CCNGG 4 cut(s) 260, 541, 847, 884
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 3 cut(s) 1084, 1219, 1425
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 1001
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 467, 814
BpuMI CCSGG 3 cut(s) 260, 541, 884
Bsa29I ATCGAT 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 1439
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 3 cut(s) 153, 166, 627
BsaXI ACNNNNNCTCC 2 cut(s) 1551, 1581
Bsc4I CCNNNNNNNGG 1 cut(s) 889
Bse1I ACTGG 2 cut(s) 118, 409
Bse8I GATNNNNATC 1 cut(s) 1439
BseBI CCWGG 1 cut(s) 847
BseCI ATCGAT 1 cut(s) 86
BseDI CCNNGG 3 cut(s) 153, 166, 627
BseGI GGATG 1 cut(s) 1234
BseJI GATNNNNATC 1 cut(s) 1439
BseLI CCNNNNNNNGG 1 cut(s) 889
BseMII CTCAG 3 cut(s) 111, 879, 1379
BseNI ACTGG 2 cut(s) 118, 409
BseRI GAGGAG 2 cut(s) 905, 1316
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 4 cut(s) 279, 801, 1358, 1469
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 2 cut(s) 176, 979
Bsh1285I CGRYCG 2 cut(s) 86, 1677
BshFI GGCC 3 cut(s) 158, 882, 1401
BshVI ATCGAT 1 cut(s) 86
BsiEI CGRYCG 2 cut(s) 86, 1677
BsiHKAI GWGCWC 3 cut(s) 655, 1108, 1471
BsiSI CCGG 4 cut(s) 190, 260, 540, 883
BslFI GGGAC 1 cut(s) 1072
BslI CCNNNNNNNGG 1 cut(s) 889
BsmAI GTCTC 3 cut(s) 156, 1306, 1414
BsmFI GGGAC 1 cut(s) 1072
BsmI GAATGC 1 cut(s) 995
BsnI GGCC 3 cut(s) 158, 882, 1401
Bso31I GGTCTC 1 cut(s) 156
Bsp1286I GDGCHC 3 cut(s) 655, 1108, 1471
Bsp1407I TGTACA 2 cut(s) 279, 607
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 2 cut(s) 979, 1402
BspANI GGCC 3 cut(s) 158, 882, 1401
BspCNI CTCAG 3 cut(s) 110, 880, 1380
BspDI ATCGAT 1 cut(s) 86
BspFNI CGCG 2 cut(s) 176, 979
BspLI GGNNCC 1 cut(s) 1545
BspPI GGATC 4 cut(s) 710, 816, 1368, 1435
BspTNI GGTCTC 1 cut(s) 156
BsrGI TGTACA 2 cut(s) 279, 607
BsrI ACTGG 2 cut(s) 118, 409
BssECI CCNNGG 3 cut(s) 153, 166, 627
BssT1I CCWWGG 1 cut(s) 627
Bst2UI CCWGG 1 cut(s) 847
Bst4CI ACNGT 5 cut(s) 368, 557, 692, 1190, 1357
Bst6I CTCTTC 1 cut(s) 1425
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstAUI TGTACA 2 cut(s) 279, 607
BstC8I GCNNGC 1 cut(s) 1399
BstDEI CTNAG 6 cut(s) 97, 338, 443, 888, 1066, 1388
BstDSI CCRYGG 1 cut(s) 627
BstEII GGTNACC 1 cut(s) 841
BstF5I GGATG 1 cut(s) 1234
BstFNI CGCG 2 cut(s) 176, 979
BstH2I RGCGCY 1 cut(s) 1202
BstHHI GCGC 3 cut(s) 464, 979, 1201
BstMAI GTCTC 3 cut(s) 156, 1306, 1414
BstMCI CGRYCG 2 cut(s) 86, 1677
BstMWI GCNNNNNNNGC 5 cut(s) 461, 569, 893, 1103, 1205
BstNI CCWGG 1 cut(s) 847
BstNSI RCATGY 5 cut(s) 638, 1078, 1208, 1222, 1281
BstPI GGTNACC 1 cut(s) 841
BstSCI CCNGG 4 cut(s) 258, 539, 845, 882
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 2 cut(s) 176, 979
BstV1I GCAGC 4 cut(s) 279, 801, 1358, 1469
BstV2I GAAGAC 1 cut(s) 1001
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
Bsu15I ATCGAT 1 cut(s) 86
BsuRI GGCC 3 cut(s) 158, 882, 1401
BsuTUI ATCGAT 1 cut(s) 86
BtgI CCRYGG 1 cut(s) 627
BtgZI GCGATG 1 cut(s) 1430
BtsCI GGATG 1 cut(s) 1234
BtsI GCAGTG 1 cut(s) 1561
BtsIMutI CAGTG 1 cut(s) 1561
Cac8I GCNNGC 1 cut(s) 1399
CfoI GCGC 3 cut(s) 464, 979, 1201
ClaI ATCGAT 1 cut(s) 86
Csp6I GTAC 4 cut(s) 144, 280, 608, 775
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviQI GTAC 4 cut(s) 144, 280, 608, 775
DdeI CTNAG 6 cut(s) 97, 338, 443, 888, 1066, 1388
EaeI YGGCCR 1 cut(s) 880
Eam1104I CTCTTC 1 cut(s) 1425
EarI CTCTTC 1 cut(s) 1425
Ecl136II GAGCTC 1 cut(s) 1469
Eco130I CCWWGG 1 cut(s) 627
Eco24I GRGCYC 1 cut(s) 1471
Eco31I GGTCTC 1 cut(s) 156
Eco47III AGCGCT 1 cut(s) 1200
Eco53kI GAGCTC 1 cut(s) 1469
Eco91I GGTNACC 1 cut(s) 841
EcoICRI GAGCTC 1 cut(s) 1469
EcoO65I GGTNACC 1 cut(s) 841
EcoRI GAATTC 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 845
EcoT14I CCWWGG 1 cut(s) 627
EcoT22I ATGCAT 1 cut(s) 1210
EcoT38I GRGCYC 1 cut(s) 1471
ErhI CCWWGG 1 cut(s) 627
FaqI GGGAC 1 cut(s) 1072
FauNDI CATATG 1 cut(s) 1018
FbaI TGATCA 1 cut(s) 231
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
FokI GGATG 1 cut(s) 1241
FriOI GRGCYC 1 cut(s) 1471
Fsp4HI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
FspI TGCGCA 1 cut(s) 463
GlaI GCGC 3 cut(s) 463, 978, 1200
GluI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
HaeII RGCGCY 1 cut(s) 1202
HaeIII GGCC 3 cut(s) 158, 882, 1401
HapII CCGG 4 cut(s) 190, 260, 540, 883
HhaI GCGC 3 cut(s) 464, 979, 1201
Hin6I GCGC 3 cut(s) 462, 977, 1199
HinP1I GCGC 3 cut(s) 462, 977, 1199
HincII GTYRAC 2 cut(s) 106, 1072
HindII GTYRAC 2 cut(s) 106, 1072
HinfI GANTC 3 cut(s) 266, 473, 1390
HpaI GTTAAC 1 cut(s) 106
HpaII CCGG 4 cut(s) 190, 260, 540, 883
HphI GGTGA 2 cut(s) 82, 616
Hpy166II GTNNAC 5 cut(s) 106, 214, 653, 688, 1072
Hpy188I TCNGA 6 cut(s) 154, 236, 913, 1001, 1311, 1389
Hpy188III TCNNGA 5 cut(s) 220, 938, 1057, 1244, 1474
Hpy8I GTNNAC 5 cut(s) 106, 214, 653, 688, 1072
HpyCH4III ACNGT 5 cut(s) 368, 557, 692, 1190, 1357
HpyF10VI GCNNNNNNNGC 5 cut(s) 461, 569, 893, 1103, 1205
HpyF3I CTNAG 6 cut(s) 97, 338, 443, 888, 1066, 1388
HspAI GCGC 3 cut(s) 462, 977, 1199
Ksp22I TGATCA 1 cut(s) 231
KspAI GTTAAC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 1466
Lsp1109I GCAGC 4 cut(s) 279, 801, 1358, 1469
LweI GCATC 3 cut(s) 1084, 1219, 1425
MaeIII GTNAC 2 cut(s) 302, 841
MboII GAAGA 8 cut(s) 16, 190, 1001, 1122, 1144, 1376, 1442, 1536
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 3 cut(s) 655, 1108, 1471
MluCI AATT 6 cut(s) 59, 148, 316, 850, 1086, 1113
MlyI GAGTC 2 cut(s) 275, 1399
MmeI TCCRAC 2 cut(s) 396, 1468
Mph1103I ATGCAT 1 cut(s) 1210
MroXI GAANNNNTTC 1 cut(s) 185
MseI TTAA 6 cut(s) 105, 122, 549, 735, 741, 1691
MslI CAYNNNNRTG 4 cut(s) 629, 1017, 1497, 1554
MspI CCGG 4 cut(s) 190, 260, 540, 883
MspR9I CCNGG 4 cut(s) 260, 541, 847, 884
Mva1269I GAATGC 1 cut(s) 995
MvaI CCWGG 1 cut(s) 847
MvnI CGCG 2 cut(s) 176, 979
MwoI GCNNNNNNNGC 5 cut(s) 461, 569, 893, 1103, 1205
NciI CCSGG 3 cut(s) 260, 541, 884
NcoI CCATGG 1 cut(s) 627
NdeI CATATG 1 cut(s) 1018
NlaIV GGNNCC 1 cut(s) 1545
NmeAIII GCCGAG 1 cut(s) 147
NsbI TGCGCA 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 1210
NspI RCATGY 5 cut(s) 638, 1078, 1208, 1222, 1281
OliI CACNNNNGTG 1 cut(s) 629
PciI ACATGT 1 cut(s) 1074
PctI GAATGC 1 cut(s) 995
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 1 cut(s) 473
PflFI GACNNNGTC 1 cut(s) 1005
PkrI GCNGC 5 cut(s) 294, 791, 1373, 1403, 1484
Ple19I CGATCG 1 cut(s) 86
PleI GAGTC 2 cut(s) 274, 1398
PpsI GAGTC 2 cut(s) 274, 1398
PscI ACATGT 1 cut(s) 1074
PsiI TTATAA 1 cut(s) 870
Psp124BI GAGCTC 1 cut(s) 1471
Psp6I CCWGG 1 cut(s) 845
PspEI GGTNACC 1 cut(s) 841
PspGI CCWGG 1 cut(s) 845
PspN4I GGNNCC 1 cut(s) 1545
PsuI RGATCY 1 cut(s) 808
PsyI GACNNNGTC 1 cut(s) 1005
PvuI CGATCG 1 cut(s) 86
RsaI GTAC 4 cut(s) 145, 281, 609, 776
RsaNI GTAC 4 cut(s) 144, 280, 608, 775
RseI CAYNNNNRTG 4 cut(s) 629, 1017, 1497, 1554
SacI GAGCTC 1 cut(s) 1471
SaqAI TTAA 6 cut(s) 105, 122, 549, 735, 741, 1691
SatI GCNGC 5 cut(s) 293, 790, 1372, 1402, 1483
SchI GAGTC 2 cut(s) 275, 1399
ScrFI CCNGG 4 cut(s) 260, 541, 847, 884
SduI GDGCHC 3 cut(s) 655, 1108, 1471
SfaNI GCATC 3 cut(s) 1084, 1219, 1425
SmiMI CAYNNNNRTG 4 cut(s) 629, 1017, 1497, 1554
SmlI CTYRAG 2 cut(s) 482, 793
SmoI CTYRAG 2 cut(s) 482, 793
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 6 cut(s) 59, 148, 316, 850, 1086, 1113
SsiI CCGC 2 cut(s) 979, 1402
SspI AATATT 1 cut(s) 1648
SstI GAGCTC 1 cut(s) 1471
StyD4I CCNGG 4 cut(s) 258, 539, 845, 882
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 5 cut(s) 368, 557, 692, 1190, 1357
TaqI TCGA 7 cut(s) 86, 567, 1025, 1108, 1393, 1600, 1677
TaqII GACCGA 2 cut(s) 1280, 1663
TasI AATT 6 cut(s) 59, 148, 316, 850, 1086, 1113
TatI WGTACW 2 cut(s) 279, 607
TauI GCSGC 1 cut(s) 1404
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 6 cut(s) 105, 122, 549, 735, 741, 1691
Tru9I TTAA 6 cut(s) 105, 122, 549, 735, 741, 1691
TscAI CASTG 1 cut(s) 1561
TseI GCWGC 4 cut(s) 292, 789, 1371, 1482
TspDTI ATGAA 7 cut(s) 17, 374, 1001, 1005, 1509, 1649, 1686
TspRI CASTG 1 cut(s) 1561
Tth111I GACNNNGTC 1 cut(s) 1005
VneI GTGCAC 1 cut(s) 651
XapI RAATTY 2 cut(s) 850, 1113
XceI RCATGY 5 cut(s) 638, 1078, 1208, 1222, 1281
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.