pycom12g21020

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010048
Physical Location & Seq
Reverse (-)
22296126 .. 22298226
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
ATGACGAAACTCGAGGGAGAGGGGAGAGGGGAGAGGGGAGAGAGAAGAGGTCTTAAAATGCAGCAACAAAATTCCAAGGGGAGATTACAGCCAGTTCTACCGAAGCGGATAATCCTAATGCGGCACGGCGAGTCCGAAGGGAATCTCGACCACGCCGCCTACACCACCACCCCCGACAACAAAATCCCACTCACCCAAGAGGGCCTGGCACAGGCCCGGCTCGCCGGGGCAGAGCTCCACCGCGTCGTCTCCGACACCACCGGCGCCGACAGCCCCAACTGGCGCGTCTACTTCTACGTCTCCCCATACGAGCGGACGCGGTCCACGCTCTGCCAGATCGGACGGTCATTCTCCAGGACGCGGATCATTGGGGTCCGCGAGGAGTGCCGGATACGGGAGCAGGATTTCGGGAACTTCCAAGTGCAGGAGCGGATGAAGGCCACTAAGGAGACCAGGGAGCGGTTCGGCCGGTTCTTCTACCGGTTTCCGGAGGGGGAGTCCGCCGCCGACGTCTACGATCGCGTTTCGAGTTTTCTCGAATCCTTATGGAGGGACATAGACATGAACAGGCTTCGCCATGACCCTTGCCACGACCTGAATCTTATAATAATATCGCACGGGTTAGCATCACGCGTCTTCCTCATGAAGTGGTTCAAGTGGACGGTCGAGCAATTTGAGCTCCTAATTAACCTTGGAAATTGCGAATTTCGAGTGATGCAATTAGGAAAGGGTGGAGAGTACAGCTTAGCAATTCATCACACTGAGGAAGAACTACAAGAATGGGGTCTCTCACCCGAAATGATAGCAGACCAAAAGTGGCGAGCCCGCGCCAACAGGGTTGATTGGACTGAACATTGCCCCTGGTACCTTGATGCTTTTTTCGACCAACTAGCTGAGTCGGAAGAAGACAACGGAGACGGTGACGAAATTTGGCATGAAACTGATGAAAAAATTCCGCAAACGAATTGA

Protein Analysis

323

Amino Acids

37.67

Weight (kDa)

5.67

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 37 - 246 1.5e-25 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AatII GACGTC 1 cut(s) 513
Acc65I GGTACC 1 cut(s) 864
AccB1I GGYRCC 2 cut(s) 263, 864
AccBSI CCGCTC 3 cut(s) 313, 430, 460
AccI GTMKAC 2 cut(s) 288, 513
AccII CGCG 8 cut(s) 243, 285, 319, 361, 378, 522, 633, 828
AccIII TCCGGA 1 cut(s) 487
AclWI GGATC 1 cut(s) 371
AcoI YGGCCR 1 cut(s) 466
AcsI RAATTY 4 cut(s) 70, 704, 927, 951
AcyI GRCGYC 2 cut(s) 264, 510
AfaI GTAC 2 cut(s) 740, 866
AfiI CCNNNNNNNGG 7 cut(s) 211, 360, 394, 424, 459, 487, 549
AflIII ACRYGT 1 cut(s) 631
AgeI ACCGGT 1 cut(s) 480
AgsI TTSAA 1 cut(s) 655
AjnI CCWGG 4 cut(s) 204, 353, 452, 860
AluBI AGCT 4 cut(s) 235, 679, 744, 893
AluI AGCT 4 cut(s) 235, 679, 744, 893
Alw21I GWGCWC 2 cut(s) 237, 681
Alw26I GTCTC 5 cut(s) 253, 304, 443, 791, 909
AlwI GGATC 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 487
AoxI GGCC 4 cut(s) 202, 213, 438, 466
ApeKI GCWGC 1 cut(s) 61
ApoI RAATTY 4 cut(s) 70, 704, 927, 951
AsiGI ACCGGT 1 cut(s) 480
Asp700I GAANNNNTTC 2 cut(s) 650, 951
Asp718I GGTACC 1 cut(s) 864
AspLEI GCGC 3 cut(s) 266, 285, 830
AspS9I GGNCC 4 cut(s) 202, 214, 321, 373
AsuC2I CCSGG 2 cut(s) 217, 226
AsuHPI GGTGA 3 cut(s) 184, 783, 932
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 2 cut(s) 321, 373
BanI GGYRCC 2 cut(s) 263, 864
BanII GRGCYC 3 cut(s) 237, 681, 826
BbsI GAAGAC 2 cut(s) 628, 912
Bbv12I GWGCWC 2 cut(s) 237, 681
BbvI GCAGC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 4 cut(s) 206, 355, 454, 862
BciVI GTATCC 1 cut(s) 384
BcnI CCSGG 2 cut(s) 217, 226
BcoDI GTCTC 5 cut(s) 253, 304, 443, 791, 909
BfaI CTAG 1 cut(s) 890
BfoI RGCGCY 1 cut(s) 267
BfuI GTATCC 1 cut(s) 384
BisI GCNGC 4 cut(s) 62, 122, 156, 504
BlpI GCTNAGC 1 cut(s) 745
BlsI GCNGC 4 cut(s) 63, 123, 157, 505
Bme1390I CCNGG 6 cut(s) 206, 217, 226, 355, 454, 862
Bme18I GGWCC 2 cut(s) 321, 373
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 4 cut(s) 202, 214, 321, 373
BmiI GGNNCC 3 cut(s) 265, 374, 866
BmrFI CCNGG 6 cut(s) 206, 217, 226, 355, 454, 862
BmsI GCATC 3 cut(s) 635, 705, 862
BpiI GAAGAC 2 cut(s) 628, 912
BpmI CTGGAG 1 cut(s) 337
Bpu1102I GCTNAGC 1 cut(s) 745
BpuMI CCSGG 2 cut(s) 217, 226
BsaHI GRCGYC 2 cut(s) 264, 510
BsaI GGTCTC 2 cut(s) 443, 791
BsaJI CCNNGG 5 cut(s) 75, 225, 453, 691, 860
BsaWI WCCGGW 2 cut(s) 480, 487
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 7 cut(s) 211, 360, 394, 424, 459, 487, 549
Bse118I RCCGGY 3 cut(s) 260, 468, 480
Bse1I ACTGG 2 cut(s) 92, 284
Bse3DI GCAATG 1 cut(s) 853
BseAI TCCGGA 1 cut(s) 487
BseBI CCWGG 4 cut(s) 206, 355, 454, 862
BseDI CCNNGG 5 cut(s) 75, 225, 453, 691, 860
BseGI GGATG 1 cut(s) 438
BseLI CCNNNNNNNGG 7 cut(s) 211, 360, 394, 424, 459, 487, 549
BseMI GCAATG 1 cut(s) 853
BseMII CTCAG 2 cut(s) 753, 885
BseNI ACTGG 2 cut(s) 92, 284
BseRI GAGGAG 1 cut(s) 395
BseX3I CGGCCG 1 cut(s) 466
BseXI GCAGC 1 cut(s) 73
BsgI GTGCAG 1 cut(s) 443
Bsh1236I CGCG 8 cut(s) 243, 285, 319, 361, 378, 522, 633, 828
Bsh1285I CGRYCG 3 cut(s) 469, 520, 666
BshFI GGCC 4 cut(s) 204, 215, 440, 468
BshNI GGYRCC 2 cut(s) 263, 864
BshTI ACCGGT 1 cut(s) 480
BsiEI CGRYCG 3 cut(s) 469, 520, 666
BsiHKAI GWGCWC 2 cut(s) 237, 681
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 7 cut(s) 217, 225, 261, 388, 469, 481, 488
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 7 cut(s) 211, 360, 394, 424, 459, 487, 549
BsmAI GTCTC 5 cut(s) 253, 304, 443, 791, 909
BsmBI CGTCTC 3 cut(s) 253, 304, 909
BsmFI GGGAC 1 cut(s) 566
BsnI GGCC 4 cut(s) 204, 215, 440, 468
Bso31I GGTCTC 2 cut(s) 443, 791
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 3 cut(s) 237, 681, 826
Bsp13I TCCGGA 1 cut(s) 487
Bsp143I GATC 3 cut(s) 336, 363, 517
Bsp1720I GCTNAGC 1 cut(s) 745
BspANI GGCC 4 cut(s) 204, 215, 440, 468
BspCNI CTCAG 2 cut(s) 754, 886
BspEI TCCGGA 1 cut(s) 487
BspFNI CGCG 8 cut(s) 243, 285, 319, 361, 378, 522, 633, 828
BspHI TCATGA 1 cut(s) 642
BspLI GGNNCC 3 cut(s) 265, 374, 866
BspPI GGATC 1 cut(s) 371
BspT107I GGYRCC 2 cut(s) 263, 864
BspTNI GGTCTC 2 cut(s) 443, 791
BsrBI CCGCTC 3 cut(s) 313, 430, 460
BsrDI GCAATG 1 cut(s) 853
BsrFI RCCGGY 3 cut(s) 260, 468, 480
BsrI ACTGG 2 cut(s) 92, 284
BssAI RCCGGY 3 cut(s) 260, 468, 480
BssECI CCNNGG 5 cut(s) 75, 225, 453, 691, 860
BssMI GATC 3 cut(s) 336, 363, 517
BssNI GRCGYC 2 cut(s) 264, 510
BssT1I CCWWGG 2 cut(s) 75, 691
Bst2UI CCWGG 4 cut(s) 206, 355, 454, 862
Bst4CI ACNGT 3 cut(s) 345, 664, 920
Bst6I CTCTTC 1 cut(s) 40
BstACI GRCGYC 2 cut(s) 264, 510
BstC8I GCNNGC 3 cut(s) 222, 822, 826
BstDEI CTNAG 4 cut(s) 444, 745, 762, 894
BstENI CCTNNNNNAGG 2 cut(s) 209, 547
BstF5I GGATG 1 cut(s) 438
BstFNI CGCG 8 cut(s) 243, 285, 319, 361, 378, 522, 633, 828
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 3 cut(s) 266, 285, 830
BstKTI GATC 3 cut(s) 339, 366, 520
BstMAI GTCTC 5 cut(s) 253, 304, 443, 791, 909
BstMBI GATC 3 cut(s) 336, 363, 517
BstMCI CGRYCG 3 cut(s) 469, 520, 666
BstMWI GCNNNNNNNGC 5 cut(s) 221, 270, 325, 384, 676
BstNI CCWGG 4 cut(s) 206, 355, 454, 862
BstSCI CCNGG 6 cut(s) 204, 215, 224, 353, 452, 860
BstUI CGCG 8 cut(s) 243, 285, 319, 361, 378, 522, 633, 828
BstV1I GCAGC 1 cut(s) 73
BstV2I GAAGAC 2 cut(s) 628, 912
BstZI CGGCCG 1 cut(s) 466
BsuI GTATCC 1 cut(s) 384
BsuRI GGCC 4 cut(s) 204, 215, 440, 468
BtsCI GGATG 1 cut(s) 438
BtsIMutI CAGTG 1 cut(s) 759
Cac8I GCNNGC 3 cut(s) 222, 822, 826
CciI TCATGA 1 cut(s) 642
CfoI GCGC 3 cut(s) 266, 285, 830
Cfr10I RCCGGY 3 cut(s) 260, 468, 480
Cfr13I GGNCC 4 cut(s) 202, 214, 321, 373
CseI GACGC 5 cut(s) 232, 274, 325, 367, 622
Csp6I GTAC 2 cut(s) 739, 865
CspAI ACCGGT 1 cut(s) 480
CviAII CATG 4 cut(s) 562, 578, 643, 935
CviQI GTAC 2 cut(s) 739, 865
DdeI CTNAG 4 cut(s) 444, 745, 762, 894
DinI GGCGCC 1 cut(s) 265
DpnI GATC 3 cut(s) 338, 365, 519
DpnII GATC 3 cut(s) 336, 363, 517
EaeI YGGCCR 1 cut(s) 466
EagI CGGCCG 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
EciI GGCGGA 1 cut(s) 490
Ecl136II GAGCTC 2 cut(s) 235, 679
EclXI CGGCCG 1 cut(s) 466
Eco130I CCWWGG 2 cut(s) 75, 691
Eco24I GRGCYC 3 cut(s) 237, 681, 826
Eco31I GGTCTC 2 cut(s) 443, 791
Eco47I GGWCC 2 cut(s) 321, 373
Eco52I CGGCCG 1 cut(s) 466
Eco53kI GAGCTC 2 cut(s) 235, 679
Eco88I CYCGRG 1 cut(s) 11
EcoICRI GAGCTC 2 cut(s) 235, 679
EcoNI CCTNNNNNAGG 2 cut(s) 209, 547
EcoO109I RGGNCCY 1 cut(s) 202
EcoRII CCWGG 4 cut(s) 204, 353, 452, 860
EcoT14I CCWWGG 2 cut(s) 75, 691
EcoT38I GRGCYC 3 cut(s) 237, 681, 826
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 75, 691
Esp3I CGTCTC 3 cut(s) 253, 304, 909
FaeI CATG 4 cut(s) 565, 581, 646, 938
FaiI YATR 8 cut(s) 307, 547, 557, 563, 579, 605, 644, 936
FaqI GGGAC 1 cut(s) 566
FatI CATG 4 cut(s) 561, 577, 642, 934
FauI CCCGC 1 cut(s) 833
FblI GTMKAC 2 cut(s) 288, 513
Fnu4HI GCNGC 4 cut(s) 62, 122, 156, 504
FokI GGATG 1 cut(s) 445
FriOI GRGCYC 3 cut(s) 237, 681, 826
Fsp4HI GCNGC 4 cut(s) 62, 122, 156, 504
FspBI CTAG 1 cut(s) 890
GlaI GCGC 3 cut(s) 265, 284, 829
GluI GCNGC 4 cut(s) 62, 122, 156, 504
GsuI CTGGAG 1 cut(s) 337
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 4 cut(s) 204, 215, 440, 468
HapII CCGG 7 cut(s) 217, 225, 261, 388, 469, 481, 488
HgaI GACGC 5 cut(s) 232, 274, 325, 367, 622
HhaI GCGC 3 cut(s) 266, 285, 830
Hin1I GRCGYC 2 cut(s) 264, 510
Hin1II CATG 4 cut(s) 565, 581, 646, 938
Hin6I GCGC 3 cut(s) 264, 283, 828
HinP1I GCGC 3 cut(s) 264, 283, 828
HinfI GANTC 6 cut(s) 131, 142, 497, 539, 598, 896
HpaII CCGG 7 cut(s) 217, 225, 261, 388, 469, 481, 488
HphI GGTGA 3 cut(s) 184, 783, 932
Hpy166II GTNNAC 4 cut(s) 289, 324, 514, 660
Hpy188I TCNGA 4 cut(s) 136, 253, 341, 901
Hpy188III TCNNGA 5 cut(s) 146, 409, 488, 536, 643
Hpy8I GTNNAC 4 cut(s) 289, 324, 514, 660
Hpy99I CGWCG 2 cut(s) 248, 512
HpyAV CCTTC 2 cut(s) 131, 430
HpyCH4III ACNGT 3 cut(s) 345, 664, 920
HpyCH4IV ACGT 2 cut(s) 297, 510
HpyCH4V TGCA 3 cut(s) 61, 424, 718
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 270, 325, 384, 676
HpyF3I CTNAG 4 cut(s) 444, 745, 762, 894
HpySE526I ACGT 2 cut(s) 297, 510
Hsp92I GRCGYC 2 cut(s) 264, 510
Hsp92II CATG 4 cut(s) 565, 581, 646, 938
HspAI GCGC 3 cut(s) 264, 283, 828
KasI GGCGCC 1 cut(s) 263
Kpn2I TCCGGA 1 cut(s) 487
KpnI GGTACC 1 cut(s) 868
Kzo9I GATC 3 cut(s) 336, 363, 517
LmnI GCTCC 5 cut(s) 240, 397, 427, 457, 684
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 3 cut(s) 635, 705, 862
MaeI CTAG 1 cut(s) 890
MaeII ACGT 2 cut(s) 297, 510
MaeIII GTNAC 1 cut(s) 920
MalI GATC 3 cut(s) 338, 365, 519
MbiI CCGCTC 3 cut(s) 313, 430, 460
MboI GATC 3 cut(s) 336, 363, 517
MboII GAAGA 6 cut(s) 57, 466, 628, 779, 914, 917
MhlI GDGCHC 3 cut(s) 237, 681, 826
MluI ACGCGT 1 cut(s) 631
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 3 cut(s) 140, 506, 905
MmeI TCCRAC 2 cut(s) 276, 879
MroI TCCGGA 1 cut(s) 487
MroXI GAANNNNTTC 2 cut(s) 650, 951
MseI TTAA 2 cut(s) 54, 687
MslI CAYNNNNRTG 1 cut(s) 560
MspI CCGG 7 cut(s) 217, 225, 261, 388, 469, 481, 488
MspR9I CCNGG 6 cut(s) 206, 217, 226, 355, 454, 862
MvaI CCWGG 4 cut(s) 206, 355, 454, 862
MvnI CGCG 8 cut(s) 243, 285, 319, 361, 378, 522, 633, 828
MwoI GCNNNNNNNGC 5 cut(s) 221, 270, 325, 384, 676
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 2 cut(s) 217, 226
NdeII GATC 3 cut(s) 336, 363, 517
NlaIII CATG 4 cut(s) 565, 581, 646, 938
NlaIV GGNNCC 3 cut(s) 265, 374, 866
NmuCI GTSAC 1 cut(s) 920
PaeR7I CTCGAG 1 cut(s) 11
PagI TCATGA 1 cut(s) 642
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 2 cut(s) 650, 951
PfeI GAWTC 3 cut(s) 142, 539, 598
PflFI GACNNNGTC 1 cut(s) 319
PfoI TCCNGGA 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 480
PkrI GCNGC 4 cut(s) 63, 123, 157, 505
Ple19I CGATCG 1 cut(s) 520
PleI GAGTC 3 cut(s) 139, 505, 904
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 3 cut(s) 139, 505, 904
PsiI TTATAA 1 cut(s) 605
Psp124BI GAGCTC 2 cut(s) 237, 681
Psp6I CCWGG 4 cut(s) 204, 353, 452, 860
PspGI CCWGG 4 cut(s) 204, 353, 452, 860
PspN4I GGNNCC 3 cut(s) 265, 374, 866
PspPI GGNCC 4 cut(s) 202, 214, 321, 373
PspXI VCTCGAGB 1 cut(s) 11
PsyI GACNNNGTC 1 cut(s) 319
PvuI CGATCG 1 cut(s) 520
RsaI GTAC 2 cut(s) 740, 866
RsaNI GTAC 2 cut(s) 739, 865
RseI CAYNNNNRTG 1 cut(s) 560
SacI GAGCTC 2 cut(s) 237, 681
SaqAI TTAA 2 cut(s) 54, 687
SatI GCNGC 4 cut(s) 62, 122, 156, 504
Sau3AI GATC 3 cut(s) 336, 363, 517
Sau96I GGNCC 4 cut(s) 202, 214, 321, 373
SchI GAGTC 3 cut(s) 140, 506, 905
ScrFI CCNGG 6 cut(s) 206, 217, 226, 355, 454, 862
SduI GDGCHC 3 cut(s) 237, 681, 826
SfaNI GCATC 3 cut(s) 635, 705, 862
SfoI GGCGCC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 11
SgrAI CRCCGGYG 1 cut(s) 260
SinI GGWCC 2 cut(s) 321, 373
SlaI CTCGAG 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 890
SstI GAGCTC 2 cut(s) 237, 681
StyD4I CCNGG 6 cut(s) 204, 215, 224, 353, 452, 860
StyI CCWWGG 2 cut(s) 75, 691
TaaI ACNGT 3 cut(s) 345, 664, 920
TaiI ACGT 2 cut(s) 300, 513
TaqI TCGA 7 cut(s) 12, 147, 527, 537, 666, 709, 882
TatI WGTACW 1 cut(s) 738
TauI GCSGC 3 cut(s) 124, 158, 506
TfiI GAWTC 3 cut(s) 142, 539, 598
Tru1I TTAA 2 cut(s) 54, 687
Tru9I TTAA 2 cut(s) 54, 687
TscAI CASTG 1 cut(s) 766
TseFI GTSAC 1 cut(s) 920
TseI GCWGC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 920
TspDTI ATGAA 6 cut(s) 449, 578, 659, 743, 951, 960
TspGWI ACGGA 1 cut(s) 927
TspRI CASTG 1 cut(s) 766
Tth111I GACNNNGTC 1 cut(s) 319
VpaK11BI GGWCC 2 cut(s) 321, 373
XagI CCTNNNNNAGG 2 cut(s) 209, 547
XapI RAATTY 4 cut(s) 70, 704, 927, 951
XhoI CTCGAG 1 cut(s) 11
XmiI GTMKAC 2 cut(s) 288, 513
XmnI GAANNNNTTC 2 cut(s) 650, 951
XspI CTAG 1 cut(s) 890
ZraI GACGTC 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.