pycom12g22140

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009806
Physical Location & Seq
Forward (+)
23153224 .. 23155303
2080 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGAACGATCAAGTGGAGGAGATGAAGAAGGAGAAGAATGTTGCTCCTGTTCCTAGGTCAAATAATCCAACTAAGCCTCCTGCTGCCAAGGCTCAAGCTGTAGGTTGGCCACCTGTCCGATCATTCCGAAAGAACATCCTGGCCGTCCAAAAGAATGGCTCTGAGGAGGGTGAAAAGACCAACAGTATCACTGCAGCATTTGTTAAAGTCAGCATGGATGGCGCGCCCTACTTGCGTAAGGTGGACCTGAAGCTGTACAAAAGCTACCAAGAGCTTTCTACTGCCTTAAGCAAAATGTTTAGCTCCTTCACCATCGGTAACTGTGGGTCACAAGGAATGAAGGATTTCGTGAACGAGAGCAAACTGATAGACCTTCTGAGTGGTTCTGAGTATGTGCCTAGTTATGAGGACAAGGATGGAGATTGGATGCTTGTTGGAGATGTGCCATGGGAAATGTTTGTCAATTCATGCAAACGCCTGCGAATAATGAAGGGATCTGAGGCTATTGGACTTGCACCAAGAGCGGTGGAGAAGTGCAAGAACAGAAGTTGCTAG

Protein Analysis

185

Amino Acids

20.43

Weight (kDa)

9.1

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 7 - 174 4.4e-83 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 524
AccII CGCG 1 cut(s) 224
AciI CCGC 1 cut(s) 524
AclWI GGATC 1 cut(s) 502
AcoI YGGCCR 2 cut(s) 107, 141
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 1 cut(s) 257
AflII CTTAAG 1 cut(s) 286
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 5 cut(s) 98, 253, 264, 274, 303
AluI AGCT 5 cut(s) 98, 253, 264, 274, 303
AlwI GGATC 1 cut(s) 502
AoxI GGCC 2 cut(s) 107, 141
ApeKI GCWGC 2 cut(s) 83, 194
AscI GGCGCGCC 1 cut(s) 222
Asp700I GAANNNNTTC 1 cut(s) 344
AspA2I CCTAGG 1 cut(s) 53
AspLEI GCGC 2 cut(s) 224, 226
AspS9I GGNCC 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 182, 301
AvaII GGWCC 1 cut(s) 244
AvrII CCTAGG 1 cut(s) 53
BalI TGGCCA 1 cut(s) 109
BbvI GCAGC 2 cut(s) 70, 206
BccI CCATC 3 cut(s) 212, 320, 410
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 140
BfaI CTAG 3 cut(s) 54, 399, 553
BfmI CTRYAG 2 cut(s) 99, 192
BfrI CTTAAG 1 cut(s) 286
BisI GCNGC 2 cut(s) 84, 195
BlnI CCTAGG 1 cut(s) 53
BlsI GCNGC 2 cut(s) 85, 196
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 78
BsaJI CCNNGG 3 cut(s) 53, 87, 446
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 3 cut(s) 53, 87, 446
BseGI GGATG 4 cut(s) 135, 223, 421, 432
BseMII CTCAG 4 cut(s) 153, 368, 378, 489
BsePI GCGCGC 1 cut(s) 222
BseRI GAGGAG 2 cut(s) 32, 179
BseXI GCAGC 2 cut(s) 70, 206
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 2 cut(s) 109, 143
BsnI GGCC 2 cut(s) 109, 143
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 3 cut(s) 7, 119, 494
Bsp19I CCATGG 1 cut(s) 446
BspACI CCGC 1 cut(s) 524
BspANI GGCC 2 cut(s) 109, 143
BspCNI CTCAG 4 cut(s) 154, 369, 379, 490
BspFNI CGCG 1 cut(s) 224
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 502
BspTI CTTAAG 1 cut(s) 286
BsrBI CCGCTC 1 cut(s) 524
BsrGI TGTACA 1 cut(s) 255
BssECI CCNNGG 3 cut(s) 53, 87, 446
BssHII GCGCGC 1 cut(s) 222
BssMI GATC 3 cut(s) 7, 119, 494
BssT1I CCWWGG 3 cut(s) 53, 87, 446
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 185, 323
BstAFI CTTAAG 1 cut(s) 286
BstAUI TGTACA 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 224, 479
BstDEI CTNAG 5 cut(s) 72, 162, 377, 387, 498
BstDSI CCRYGG 1 cut(s) 446
BstF5I GGATG 4 cut(s) 135, 223, 421, 432
BstFNI CGCG 1 cut(s) 224
BstHHI GCGC 2 cut(s) 224, 226
BstKTI GATC 3 cut(s) 10, 122, 497
BstMBI GATC 3 cut(s) 7, 119, 494
BstMWI GCNNNNNNNGC 4 cut(s) 89, 219, 232, 521
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 2 cut(s) 99, 192
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 70, 206
BstX2I RGATCY 1 cut(s) 494
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 1 cut(s) 494
BsuRI GGCC 2 cut(s) 109, 143
BtgI CCRYGG 1 cut(s) 446
BtsCI GGATG 4 cut(s) 135, 223, 421, 432
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 224, 479
CfoI GCGC 2 cut(s) 224, 226
Cfr13I GGNCC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 256
CspCI CAANNNNNGTGG 2 cut(s) 507, 542
CviAII CATG 3 cut(s) 214, 447, 468
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 5 cut(s) 72, 162, 377, 387, 498
DpnI GATC 3 cut(s) 9, 121, 496
DpnII GATC 3 cut(s) 7, 119, 494
EaeI YGGCCR 2 cut(s) 107, 141
Eco130I CCWWGG 3 cut(s) 53, 87, 446
Eco47I GGWCC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 3 cut(s) 53, 87, 446
ErhI CCWWGG 3 cut(s) 53, 87, 446
FaeI CATG 3 cut(s) 217, 450, 471
FaiI YATR 5 cut(s) 215, 393, 405, 448, 469
FatI CATG 3 cut(s) 213, 446, 467
Fnu4HI GCNGC 2 cut(s) 84, 195
FokI GGATG 4 cut(s) 122, 230, 428, 439
Fsp4HI GCNGC 2 cut(s) 84, 195
FspBI CTAG 3 cut(s) 54, 399, 553
GlaI GCGC 2 cut(s) 223, 225
GluI GCNGC 2 cut(s) 84, 195
HaeIII GGCC 2 cut(s) 109, 143
HhaI GCGC 2 cut(s) 224, 226
Hin1II CATG 3 cut(s) 217, 450, 471
Hin6I GCGC 2 cut(s) 222, 224
HinP1I GCGC 2 cut(s) 222, 224
HphI GGTGA 2 cut(s) 182, 301
Hpy166II GTNNAC 2 cut(s) 244, 352
Hpy188I TCNGA 6 cut(s) 119, 128, 163, 378, 388, 499
Hpy188III TCNNGA 1 cut(s) 349
Hpy8I GTNNAC 2 cut(s) 244, 352
HpyAV CCTTC 5 cut(s) 22, 316, 334, 383, 484
HpyCH4III ACNGT 2 cut(s) 185, 323
HpyCH4V TGCA 4 cut(s) 194, 471, 515, 537
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 219, 232, 521
HpyF3I CTNAG 5 cut(s) 72, 162, 377, 387, 498
Hsp92II CATG 3 cut(s) 217, 450, 471
HspAI GCGC 2 cut(s) 222, 224
Kzo9I GATC 3 cut(s) 7, 119, 494
LmnI GCTCC 2 cut(s) 49, 308
LpnPI CCDG 7 cut(s) 60, 93, 125, 126, 152, 260, 491
Lsp1109I GCAGC 2 cut(s) 70, 206
LweI GCATC 1 cut(s) 417
MaeI CTAG 3 cut(s) 54, 399, 553
MaeIII GTNAC 2 cut(s) 317, 327
MalI GATC 3 cut(s) 9, 121, 496
MbiI CCGCTC 1 cut(s) 524
MboI GATC 3 cut(s) 7, 119, 494
MboII GAAGA 2 cut(s) 37, 46
MflI RGATCY 1 cut(s) 494
MlsI TGGCCA 1 cut(s) 109
MluCI AATT 1 cut(s) 463
MluNI TGGCCA 1 cut(s) 109
MmeI TCCRAC 2 cut(s) 92, 415
MnlI CCTC 6 cut(s) 10, 87, 157, 160, 400, 493
Mox20I TGGCCA 1 cut(s) 109
MroXI GAANNNNTTC 1 cut(s) 344
MscI TGGCCA 1 cut(s) 109
MseI TTAA 2 cut(s) 204, 287
Msp20I TGGCCA 1 cut(s) 109
MspCI CTTAAG 1 cut(s) 286
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 4 cut(s) 89, 219, 232, 521
NcoI CCATGG 1 cut(s) 446
NdeII GATC 3 cut(s) 7, 119, 494
NlaIII CATG 3 cut(s) 217, 450, 471
NmuCI GTSAC 1 cut(s) 327
PalAI GGCGCGCC 1 cut(s) 222
PauI GCGCGC 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 344
PkrI GCNGC 2 cut(s) 85, 196
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspPI GGNCC 1 cut(s) 244
PstI CTGCAG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 494
PteI GCGCGC 1 cut(s) 222
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 204, 287
SatI GCNGC 2 cut(s) 84, 195
Sau3AI GATC 3 cut(s) 7, 119, 494
Sau96I GGNCC 1 cut(s) 244
ScrFI CCNGG 1 cut(s) 140
SfaNI GCATC 1 cut(s) 417
SfcI CTRYAG 2 cut(s) 99, 192
SgsI GGCGCGCC 1 cut(s) 222
SinI GGWCC 1 cut(s) 244
SmlI CTYRAG 2 cut(s) 93, 286
SmoI CTYRAG 2 cut(s) 93, 286
Sse9I AATT 1 cut(s) 463
SsiI CCGC 1 cut(s) 524
SspMI CTAG 3 cut(s) 54, 399, 553
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 3 cut(s) 53, 87, 446
TaaI ACNGT 2 cut(s) 185, 323
TasI AATT 1 cut(s) 463
TatI WGTACW 1 cut(s) 255
Tru1I TTAA 2 cut(s) 204, 287
Tru9I TTAA 2 cut(s) 204, 287
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 327
TseI GCWGC 2 cut(s) 83, 194
Tsp45I GTSAC 1 cut(s) 327
TspDTI ATGAA 5 cut(s) 17, 38, 353, 456, 503
TspRI CASTG 1 cut(s) 196
Vha464I CTTAAG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 244
XmaJI CCTAGG 1 cut(s) 53
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 3 cut(s) 54, 399, 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.