Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0004329

Physical Location & Seq

Genomic Coordinates Reverse (-)

23368693 .. 23369589

Gene Structure 897 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g22490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGGCGAACCGGTGGTGGGCTGGACAGGTAGGTCTACCGGGAGGAGCAGTGAACGAAACCTCAGCCGCAGCAGGTGCCGGCAGCTCCCCCATGAAAAACCTCATGAAACCAGATCTGGGGATCTCCATGAACACCACCGGAACCAGCAGCCTCGGAGGATCCGGCGGTGACGAAGACGACAACAACAGCGACGATCCCAAGGAAGGAGCCATCGAAGTCGCCACAAGACGACCTAGAGGCCGTCCCCCCGGCTCCAAAAACAAGCCCAAACCCCCAATCTTTGTGACTAGAGATAGCCCTAACGCCCTCCGCAGCCACGTCATGGAGATATCCAGTGGGGCCGACATCGCCGACAGCGTCGCCCAGTTCGCCAGGAGAAGGCAGCGAGGCGTCTGCGTCATGAGTGGGAGCGGCACCGTCATCAACGTCACGCTCAGGCAGCCGTCCGCGCCCGGCGCGGTCATGGCGCTTCACGGGAGGTTCGAGATTTTGTCCTTAACTGGAGCGTTTTTGCCTGGCCCTGCACTGCCTGGTTCAACCGGGTTGACCATATATTTGGCAGGCGGGCAAGGCCAGGTTGTTGGAGGAAGCGTGGTTGGCCCCCTTGTGGCTTCAGGGCCCGTGATGGTGATCGCGGCAACGTTTTCTAATGCGACGTATGAGAGGTTGCCATTGGAGGAGGAAGAAGAGCAAACAGGAGGGGGTACTAGTTCGGGGACTGCAGCAGGAGGTGCGGGGTCGCCGCCGGGGATTGGAGGTGGTGGGATGGGAGACCCGGCGGCATTGGCAGGGGTTTATAATTTGCCATCGAATATGCTTCCGAATTTGAGCCTTGATCAGCAGGCAGCTTATTCATCTTGGGCTCATGGTCAAGGTGGTGGTAGGCCTCCATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003677 GO:0003680 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0031348 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045088 GO:0045824 GO:0048519 GO:0048583 GO:0048585 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF296

Protein Analysis

299

Amino Acids

29.89

Weight (kDa)

5.94

Isoelectric Point (pI)

50.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCC	PF03479	104 - 216	8e-24	Plants and Prokaryotes Conserved (PCC) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004329)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14465
fragaria_vesca	FvH4_4g03050 FvH4_6g02320
malus_domestica	MD04G1228100.v1.1 MD12G1245400.v1.1 MD13G1209900.v1.1 MD16G1212800.v1.1
prunus_persica	Prupe.1G034400_v2.0.a1 Prupe.6G348200_v2.0.a1
pyrus_communis	pycom04g20250 pycom12g22490 pycom13g18250
rosa_chinensis	RchiOBHm_Chr3g0450081 RchiOBHm_Chr4g0391331
rosa_laevigata	RLG00000009872 RLG00000025758
rosa_multiflora	Rmu_sc0002646.1_g000031 Rmu_sc0005322.1_g000030
rosa_roxburghii	Rroxscaffold_5G00337070 Rroxscaffold_6G00427860
rosa_rugosa	Rorug02G0625300 Rorug03G0335400
rosa_samantha	Rh3AG024400 Rh3BG025000 Rh3CG023900 Rh3DG025000 Rh4AG036500 Rh4BG031200 Rh4CG039800 Rh4DG034200
rosa_wichuraiana	Rw3G001950 Rw4G002860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	798
AarI	CACCTGC	1 cut(s)	62
AasI	GACNNNNNNGTC	1 cut(s)	30
Acc36I	ACCTGC	1 cut(s)	62
AccB1I	GGYRCC	2 cut(s)	74, 413
AccB7I	CCANNNNNTGG	1 cut(s)	322
AccBSI	CCGCTC	1 cut(s)	411
AccI	GTMKAC	1 cut(s)	34
AccII	CGCG	3 cut(s)	449, 458, 635
AclI	AACGTT	1 cut(s)	641
AclWI	GGATC	4 cut(s)	128, 153, 166, 188
AcsI	RAATTY	1 cut(s)	823
AcuI	CTGAAG	1 cut(s)	597
AcyI	GRCGYC	1 cut(s)	390
AfaI	GTAC	1 cut(s)	706
AfiI	CCNNNNNNNGG	7 cut(s)	16, 116, 203, 322, 378, 607, 752
AgeI	ACCGGT	1 cut(s)	9
AgsI	TTSAA	1 cut(s)	537
AhlI	ACTAGT	1 cut(s)	707
AjiI	CACGTC	1 cut(s)	319
AjnI	CCWGG	4 cut(s)	371, 514, 529, 573
AluBI	AGCT	2 cut(s)	84, 848
AluI	AGCT	2 cut(s)	84, 848
Alw26I	GTCTC	1 cut(s)	765
AlwI	GGATC	4 cut(s)	128, 153, 166, 188
AoxI	GGCC	7 cut(s)	238, 339, 517, 571, 598, 617, 884
ApaI	GGGCCC	1 cut(s)	621
ApeKI	GCWGC	8 cut(s)	68, 81, 147, 312, 382, 439, 722, 845
ApoI	RAATTY	1 cut(s)	823
ArsI	GACNNNNNNTTYG	2 cut(s)	538, 570
AsiGI	ACCGGT	1 cut(s)	9
AspLEI	GCGC	3 cut(s)	451, 458, 469
AspS9I	GGNCC	5 cut(s)	339, 518, 599, 617, 618
AsuC2I	CCSGG	6 cut(s)	39, 249, 453, 541, 747, 776
AsuHPI	GGTGA	2 cut(s)	179, 640
BaeGI	GKGCMC	1 cut(s)	621
BamHI	GGATCC	1 cut(s)	158
BanI	GGYRCC	2 cut(s)	74, 413
BanII	GRGCYC	2 cut(s)	621, 865
BbsI	GAAGAC	1 cut(s)	180
BbvCI	CCTCAGC	1 cut(s)	61
BbvI	GCAGC	8 cut(s)	80, 93, 159, 324, 394, 451, 734, 857
BccI	CCATC	4 cut(s)	218, 619, 760, 814
BceAI	ACGGC	2 cut(s)	225, 427
BcgI	CGANNNNNNTGC	2 cut(s)	375, 409
BciT130I	CCWGG	4 cut(s)	373, 516, 531, 575
BclI	TGATCA	1 cut(s)	835
BcnI	CCSGG	6 cut(s)	39, 249, 453, 541, 747, 776
BcoDI	GTCTC	1 cut(s)	765
BcuI	ACTAGT	1 cut(s)	707
BfaI	CTAG	3 cut(s)	234, 288, 708
BfmI	CTRYAG	1 cut(s)	720
BfoI	RGCGCY	1 cut(s)	470
BfuAI	ACCTGC	1 cut(s)	62
BglII	AGATCT	1 cut(s)	112
BmgBI	CACGTC	1 cut(s)	319
BmgT120I	GGNCC	5 cut(s)	339, 518, 599, 617, 618
BmiI	GGNNCC	9 cut(s)	76, 142, 160, 208, 253, 340, 415, 601, 619
BmrI	ACTGGG	1 cut(s)	358
BmuI	ACTGGG	1 cut(s)	358
BpiI	GAAGAC	1 cut(s)	180
BpmI	CTGGAG	1 cut(s)	522
Bpu10I	CCTNAGC	2 cut(s)	61, 434
BpuMI	CCSGG	6 cut(s)	39, 249, 453, 541, 747, 776
BsaBI	GATNNNNATC	1 cut(s)	629
BsaHI	GRCGYC	1 cut(s)	390
BsaI	GGTCTC	1 cut(s)	765
BsaJI	CCNNGG	4 cut(s)	151, 198, 247, 746
BsaWI	WCCGGW	2 cut(s)	9, 137
BsaXI	ACNNNNNCTCC	4 cut(s)	33, 63, 576, 606
Bsc4I	CCNNNNNNNGG	7 cut(s)	16, 116, 203, 322, 378, 607, 752
Bse118I	RCCGGY	2 cut(s)	9, 77
Bse1I	ACTGG	3 cut(s)	333, 364, 505
Bse8I	GATNNNNATC	1 cut(s)	629
BseBI	CCWGG	4 cut(s)	373, 516, 531, 575
BseDI	CCNNGG	4 cut(s)	151, 198, 247, 746
BseGI	GGATG	1 cut(s)	771
BseJI	GATNNNNATC	1 cut(s)	629
BseLI	CCNNNNNNNGG	7 cut(s)	16, 116, 203, 322, 378, 607, 752
BseMII	CTCAG	2 cut(s)	75, 448
BseNI	ACTGG	3 cut(s)	333, 364, 505
BseRI	GAGGAG	2 cut(s)	57, 692
BseSI	GKGCMC	1 cut(s)	621
BseXI	GCAGC	8 cut(s)	80, 93, 159, 324, 394, 451, 734, 857
BsgI	GTGCAG	1 cut(s)	507
Bsh1236I	CGCG	3 cut(s)	449, 458, 635
BshFI	GGCC	7 cut(s)	240, 341, 519, 573, 600, 619, 886
BshNI	GGYRCC	2 cut(s)	74, 413
BshTI	ACCGGT	1 cut(s)	9
BslFI	GGGAC	2 cut(s)	228, 730
BslI	CCNNNNNNNGG	7 cut(s)	16, 116, 203, 322, 378, 607, 752
BsmAI	GTCTC	1 cut(s)	765
BsmFI	GGGAC	2 cut(s)	228, 730
BsnI	GGCC	7 cut(s)	240, 341, 519, 573, 600, 619, 886
Bso31I	GGTCTC	1 cut(s)	765
Bsp120I	GGGCCC	1 cut(s)	617
Bsp1286I	GDGCHC	2 cut(s)	621, 865
Bsp143I	GATC	6 cut(s)	112, 120, 158, 193, 630, 835
BspANI	GGCC	7 cut(s)	240, 341, 519, 573, 600, 619, 886
BspCNI	CTCAG	2 cut(s)	74, 447
BspFNI	CGCG	3 cut(s)	449, 458, 635
BspHI	TCATGA	2 cut(s)	102, 399
BspLI	GGNNCC	9 cut(s)	76, 142, 160, 208, 253, 340, 415, 601, 619
BspMAI	CTGCAG	1 cut(s)	724
BspMI	ACCTGC	1 cut(s)	62
BspPI	GGATC	4 cut(s)	128, 153, 166, 188
BspQI	GCTCTTC	1 cut(s)	681
BspT107I	GGYRCC	2 cut(s)	74, 413
BspTNI	GGTCTC	1 cut(s)	765
BsrBI	CCGCTC	1 cut(s)	411
BsrFI	RCCGGY	2 cut(s)	9, 77
BsrI	ACTGG	3 cut(s)	333, 364, 505
BssAI	RCCGGY	2 cut(s)	9, 77
BssECI	CCNNGG	4 cut(s)	151, 198, 247, 746
BssMI	GATC	6 cut(s)	112, 120, 158, 193, 630, 835
BssNI	GRCGYC	1 cut(s)	390
BssT1I	CCWWGG	1 cut(s)	198
Bst2UI	CCWGG	4 cut(s)	373, 516, 531, 575
Bst4CI	ACNGT	1 cut(s)	418
Bst6I	CTCTTC	1 cut(s)	681
BstACI	GRCGYC	1 cut(s)	390
BstAPI	GCANNNNNTGC	2 cut(s)	74, 731
BstC8I	GCNNGC	4 cut(s)	79, 562, 566, 843
BstDEI	CTNAG	2 cut(s)	61, 434
BstF5I	GGATG	1 cut(s)	771
BstFNI	CGCG	3 cut(s)	449, 458, 635
BstH2I	RGCGCY	1 cut(s)	470
BstHHI	GCGC	3 cut(s)	451, 458, 469
BstKTI	GATC	6 cut(s)	115, 123, 161, 196, 633, 838
BstMAI	GTCTC	1 cut(s)	765
BstMBI	GATC	6 cut(s)	112, 120, 158, 193, 630, 835
BstNI	CCWGG	4 cut(s)	373, 516, 531, 575
BstSFI	CTRYAG	1 cut(s)	720
BstSLI	GKGCMC	1 cut(s)	621
BstUI	CGCG	3 cut(s)	449, 458, 635
BstV1I	GCAGC	8 cut(s)	80, 93, 159, 324, 394, 451, 734, 857
BstV2I	GAAGAC	1 cut(s)	180
BstX2I	RGATCY	3 cut(s)	112, 120, 158
BstXI	CCANNNNNNTGG	2 cut(s)	556, 581
BstYI	RGATCY	3 cut(s)	112, 120, 158
BsuRI	GGCC	7 cut(s)	240, 341, 519, 573, 600, 619, 886
BtgZI	GCGATG	1 cut(s)	331
BtrI	CACGTC	1 cut(s)	319
BtsCI	GGATG	1 cut(s)	771
BtsI	GCAGTG	2 cut(s)	54, 524
BtsIMutI	CAGTG	3 cut(s)	54, 340, 524
BveI	ACCTGC	1 cut(s)	62
Cac8I	GCNNGC	4 cut(s)	79, 562, 566, 843
CciI	TCATGA	2 cut(s)	102, 399
CfoI	GCGC	3 cut(s)	451, 458, 469
Cfr10I	RCCGGY	2 cut(s)	9, 77
Cfr13I	GGNCC	5 cut(s)	339, 518, 599, 617, 618
CseI	GACGC	3 cut(s)	346, 379, 385
Csp6I	GTAC	1 cut(s)	705
CspAI	ACCGGT	1 cut(s)	9
CviAII	CATG	7 cut(s)	91, 103, 127, 322, 400, 463, 866
CviQI	GTAC	1 cut(s)	705
DdeI	CTNAG	2 cut(s)	61, 434
DpnI	GATC	6 cut(s)	114, 122, 160, 195, 632, 837
DpnII	GATC	6 cut(s)	112, 120, 158, 193, 630, 835
DrdI	GACNNNNNNGTC	1 cut(s)	30
DseDI	GACNNNNNNGTC	1 cut(s)	30
Eam1104I	CTCTTC	1 cut(s)	681
EarI	CTCTTC	1 cut(s)	681
Eco130I	CCWWGG	1 cut(s)	198
Eco147I	AGGCCT	1 cut(s)	886
Eco24I	GRGCYC	2 cut(s)	621, 865
Eco31I	GGTCTC	1 cut(s)	765
Eco32I	GATATC	1 cut(s)	330
Eco57I	CTGAAG	1 cut(s)	597
EcoO109I	RGGNCCY	1 cut(s)	617
EcoRII	CCWGG	4 cut(s)	371, 514, 529, 573
EcoRV	GATATC	1 cut(s)	330
EcoT14I	CCWWGG	1 cut(s)	198
EcoT38I	GRGCYC	2 cut(s)	621, 865
ErhI	CCWWGG	1 cut(s)	198
FaeI	CATG	7 cut(s)	94, 106, 130, 325, 403, 466, 869
FaqI	GGGAC	2 cut(s)	228, 730
FatI	CATG	7 cut(s)	90, 102, 126, 321, 399, 462, 865
FauI	CCCGC	2 cut(s)	557, 727
FbaI	TGATCA	1 cut(s)	835
FblI	GTMKAC	1 cut(s)	34
FokI	GGATG	1 cut(s)	778
FriOI	GRGCYC	2 cut(s)	621, 865
FspBI	CTAG	3 cut(s)	234, 288, 708
GlaI	GCGC	3 cut(s)	450, 457, 468
GsuI	CTGGAG	1 cut(s)	522
HaeII	RGCGCY	1 cut(s)	470
HaeIII	GGCC	7 cut(s)	240, 341, 519, 573, 600, 619, 886
HgaI	GACGC	3 cut(s)	346, 379, 385
HhaI	GCGC	3 cut(s)	451, 458, 469
Hin1I	GRCGYC	1 cut(s)	390
Hin1II	CATG	7 cut(s)	94, 106, 130, 325, 403, 466, 869
Hin6I	GCGC	3 cut(s)	449, 456, 467
HinP1I	GCGC	3 cut(s)	449, 456, 467
HincII	GTYRAC	1 cut(s)	546
HindII	GTYRAC	1 cut(s)	546
HphI	GGTGA	2 cut(s)	179, 640
Hpy166II	GTNNAC	3 cut(s)	35, 52, 546
Hpy188I	TCNGA	2 cut(s)	155, 822
Hpy188III	TCNNGA	3 cut(s)	103, 400, 484
Hpy8I	GTNNAC	3 cut(s)	35, 52, 546
Hpy99I	CGWCG	3 cut(s)	194, 362, 658
HpyAV	CCTTC	2 cut(s)	197, 372
HpyCH4III	ACNGT	1 cut(s)	418
HpyCH4IV	ACGT	4 cut(s)	318, 426, 641, 656
HpyCH4V	TGCA	2 cut(s)	524, 722
HpyF3I	CTNAG	2 cut(s)	61, 434
HpySE526I	ACGT	4 cut(s)	318, 426, 641, 656
Hsp92I	GRCGYC	1 cut(s)	390
Hsp92II	CATG	7 cut(s)	94, 106, 130, 325, 403, 466, 869
HspAI	GCGC	3 cut(s)	449, 456, 467
KroI	GCCGGC	1 cut(s)	77
KroNI	GCCGGC	1 cut(s)	79
Ksp22I	TGATCA	1 cut(s)	835
Kzo9I	GATC	6 cut(s)	112, 120, 158, 193, 630, 835
LguI	GCTCTTC	1 cut(s)	681
LmnI	GCTCC	6 cut(s)	44, 89, 206, 257, 408, 503
Lsp1109I	GCAGC	8 cut(s)	80, 93, 159, 324, 394, 451, 734, 857
MaeI	CTAG	3 cut(s)	234, 288, 708
MaeII	ACGT	4 cut(s)	318, 426, 641, 656
MaeIII	GTNAC	3 cut(s)	167, 283, 427
MalI	GATC	6 cut(s)	114, 122, 160, 195, 632, 837
MbiI	CCGCTC	1 cut(s)	411
MboI	GATC	6 cut(s)	112, 120, 158, 193, 630, 835
MboII	GAAGA	3 cut(s)	185, 695, 698
MflI	RGATCY	3 cut(s)	112, 120, 158
MhlI	GDGCHC	2 cut(s)	621, 865
MluCI	AATT	2 cut(s)	799, 823
MmeI	TCCRAC	1 cut(s)	562
MroNI	GCCGGC	1 cut(s)	77
MseI	TTAA	2 cut(s)	497, 895
MvaI	CCWGG	4 cut(s)	373, 516, 531, 575
MvnI	CGCG	3 cut(s)	449, 458, 635
NaeI	GCCGGC	1 cut(s)	79
NciI	CCSGG	6 cut(s)	39, 249, 453, 541, 747, 776
NdeII	GATC	6 cut(s)	112, 120, 158, 193, 630, 835
NgoMIV	GCCGGC	1 cut(s)	77
NlaIII	CATG	7 cut(s)	94, 106, 130, 325, 403, 466, 869
NlaIV	GGNNCC	9 cut(s)	76, 142, 160, 208, 253, 340, 415, 601, 619
NmuCI	GTSAC	3 cut(s)	167, 283, 427
PagI	TCATGA	2 cut(s)	102, 399
PaqCI	CACCTGC	1 cut(s)	62
PceI	AGGCCT	1 cut(s)	886
PciSI	GCTCTTC	1 cut(s)	681
PcsI	WCGNNNNNNNCGW	2 cut(s)	354, 480
PdiI	GCCGGC	1 cut(s)	79
PflFI	GACNNNGTC	1 cut(s)	356
PflMI	CCANNNNNTGG	1 cut(s)	322
PinAI	ACCGGT	1 cut(s)	9
PsiI	TTATAA	1 cut(s)	798
Psp1406I	AACGTT	1 cut(s)	641
Psp6I	CCWGG	4 cut(s)	371, 514, 529, 573
PspGI	CCWGG	4 cut(s)	371, 514, 529, 573
PspN4I	GGNNCC	9 cut(s)	76, 142, 160, 208, 253, 340, 415, 601, 619
PspOMI	GGGCCC	1 cut(s)	617
PspPI	GGNCC	5 cut(s)	339, 518, 599, 617, 618
PstI	CTGCAG	1 cut(s)	724
PsuI	RGATCY	3 cut(s)	112, 120, 158
PsyI	GACNNNGTC	1 cut(s)	356
RsaI	GTAC	1 cut(s)	706
RsaNI	GTAC	1 cut(s)	705
SapI	GCTCTTC	1 cut(s)	681
SaqAI	TTAA	2 cut(s)	497, 895
Sau3AI	GATC	6 cut(s)	112, 120, 158, 193, 630, 835
Sau96I	GGNCC	5 cut(s)	339, 518, 599, 617, 618
SduI	GDGCHC	2 cut(s)	621, 865
SfcI	CTRYAG	1 cut(s)	720
SpeI	ACTAGT	1 cut(s)	707
Sse9I	AATT	2 cut(s)	799, 823
SseBI	AGGCCT	1 cut(s)	886
SspMI	CTAG	3 cut(s)	234, 288, 708
StuI	AGGCCT	1 cut(s)	886
StyI	CCWWGG	1 cut(s)	198
TaaI	ACNGT	1 cut(s)	418
TaiI	ACGT	4 cut(s)	321, 429, 644, 659
TaqI	TCGA	3 cut(s)	213, 483, 809
TasI	AATT	2 cut(s)	799, 823
TauI	GCSGC	5 cut(s)	68, 414, 638, 745, 782
Tru1I	TTAA	2 cut(s)	497, 895
Tru9I	TTAA	2 cut(s)	497, 895
TscAI	CASTG	3 cut(s)	54, 340, 531
TseFI	GTSAC	3 cut(s)	167, 283, 427
TseI	GCWGC	8 cut(s)	68, 81, 147, 312, 382, 439, 722, 845
Tsp45I	GTSAC	3 cut(s)	167, 283, 427
TspDTI	ATGAA	4 cut(s)	107, 119, 143, 843
TspRI	CASTG	3 cut(s)	54, 340, 531
Tth111I	GACNNNGTC	1 cut(s)	356
Van91I	CCANNNNNTGG	1 cut(s)	322
XapI	RAATTY	1 cut(s)	823
XmiI	GTMKAC	1 cut(s)	34
XspI	CTAG	3 cut(s)	234, 288, 708

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.