pycom12g23120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010946
Physical Location & Seq
Forward (+)
23781945 .. 23784461
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
ATGGCTTCTTCTCTTGCCTTCCTTCAACCTCTCTCCAATTTCTCTTCCTCTTCAACCCTCCTCTTTCTCCGTAATCCCCACCATATCCCTTCTTCTTCTTCTTCCTCCTTAAAACCCTCCAACTTCCTCAAAACCCTCCCATTCAAATCCCTCACCGCCTCATTCTCCCTCGCAGAATCAGACTCCCCCAAATCTTTCCAAACCAACGGTCCAAATTTTCAATCTTTCCTCCAAGAATTAGCCGACAGCTTTGATCTTCCACCGGACTACTTTGCGCAGCTTCCTAACGATCTCCGGCTCGATCTGAATGATGCAGCATTTGATCTTTCAAATGGAAGGGTTATCGACGAGTGTGGTCAAGAGTTAGGAGAGACATTGTTAAATCTCTCGCGCGCATGGGAACAAGCCGATACATCAACTTCCCATGCTTTAGCTAGCAAGCTCCCTGGGTTGGAGGACTCTATGACAGGCAATGCCAAATCAGCATTTGGAAAGCGTTTGGTTTCTGCTGGAAGAAGATTTCAGTCTATGGGACAGTATGGCCAAGGTGAACTGCAAAAGATTGCAAAAGTAATGATTACAACCGGAAAGCTTCTGTCTGAAAGTTCAACATCGACAGTCACTGATGAACCAAAGACGGAAAGCAGGATGCTGAAGTTTGGAGAACTTCAGCTCGAGCTAACATCAGATAAAGCCACCATAGGGGCCGTAATCAGCTTTGCTTTTGGGATTCTTTCATGGGAGCTAGCACAGGGTATCCAAAACATCCCGGAGAGTTCATTGCAGTACGCAAATGACAATGCTTTGATGCTGGCTAAGTCTTTGAGGGGCGCTCTGCTTACTATCTGCTATTCCTCAGCGATATTGTCTGCTTTTACTACCGTCGGACTTCTCTTACTCGGAAGACAACTAAAGTCACCAAACGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3082

Protein Analysis

311

Amino Acids

33.52

Weight (kDa)

5.5

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 276
AccII CGCG 2 cut(s) 391, 393
AciI CCGC 1 cut(s) 156
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 2 cut(s) 653, 674
AfaI GTAC 1 cut(s) 788
AfiI CCNNNNNNNGG 2 cut(s) 451, 702
AgsI TTSAA 6 cut(s) 26, 54, 145, 221, 330, 609
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 9 cut(s) 249, 280, 434, 442, 592, 673, 679, 717, 745
AluI AGCT 9 cut(s) 249, 280, 434, 442, 592, 673, 679, 717, 745
Alw26I GTCTC 1 cut(s) 365
AlwNI CAGNNNCTG 1 cut(s) 623
Ama87I CYCGRG 1 cut(s) 674
AoxI GGCC 2 cut(s) 541, 705
ApeKI GCWGC 2 cut(s) 277, 314
ApoI RAATTY 1 cut(s) 214
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AspLEI GCGC 4 cut(s) 277, 393, 395, 833
AspS9I GGNCC 2 cut(s) 209, 705
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 3 cut(s) 145, 560, 909
AsuNHI GCTAGC 2 cut(s) 434, 745
AvaI CYCGRG 1 cut(s) 674
AvaII GGWCC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 543
BbsI GAAGAC 1 cut(s) 910
BbvCI CCTCAGC 1 cut(s) 856
BbvI GCAGC 2 cut(s) 289, 326
BceAI ACGGC 1 cut(s) 692
BciT130I CCWGG 1 cut(s) 447
BciVI GTATCC 1 cut(s) 767
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 2 cut(s) 435, 746
BfoI RGCGCY 1 cut(s) 834
BfuI GTATCC 1 cut(s) 767
BisI GCNGC 2 cut(s) 278, 315
BlsI GCNGC 2 cut(s) 279, 316
Bme1390I CCNGG 2 cut(s) 447, 770
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 674
BmgT120I GGNCC 2 cut(s) 209, 705
BmiI GGNNCC 1 cut(s) 706
BmrFI CCNGG 2 cut(s) 447, 770
BmsI GCATC 3 cut(s) 301, 639, 798
BmtI GCTAGC 2 cut(s) 438, 749
BpiI GAAGAC 1 cut(s) 910
BplI GAGNNNNNCTC 2 cut(s) 817, 849
Bpu10I CCTNAGC 1 cut(s) 856
BpuMI CCSGG 1 cut(s) 770
BsaJI CCNNGG 3 cut(s) 445, 446, 544
BsaWI WCCGGW 2 cut(s) 262, 584
Bsc4I CCNNNNNNNGG 2 cut(s) 451, 702
Bse3DI GCAATG 2 cut(s) 478, 779
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 3 cut(s) 445, 446, 544
BseGI GGATG 2 cut(s) 654, 765
BseLI CCNNNNNNNGG 2 cut(s) 451, 702
BseMI GCAATG 2 cut(s) 478, 779
BseMII CTCAG 1 cut(s) 870
BsePI GCGCGC 1 cut(s) 391
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 289, 326
Bsh1236I CGCG 2 cut(s) 391, 393
BshFI GGCC 2 cut(s) 543, 707
BsiHKCI CYCGRG 1 cut(s) 674
BsiSI CCGG 4 cut(s) 263, 295, 585, 770
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 2 cut(s) 451, 702
BsmAI GTCTC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 2 cut(s) 543, 707
BsoBI CYCGRG 1 cut(s) 674
Bsp143I GATC 4 cut(s) 253, 289, 301, 322
BspACI CCGC 1 cut(s) 156
BspANI GGCC 2 cut(s) 543, 707
BspCNI CTCAG 1 cut(s) 869
BspFNI CGCG 2 cut(s) 391, 393
BspLI GGNNCC 1 cut(s) 706
BspOI GCTAGC 2 cut(s) 438, 749
BsrDI GCAATG 2 cut(s) 478, 779
BssECI CCNNGG 3 cut(s) 445, 446, 544
BssHII GCGCGC 1 cut(s) 391
BssMI GATC 4 cut(s) 253, 289, 301, 322
BssT1I CCWWGG 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 4 cut(s) 209, 537, 619, 883
Bst6I CTCTTC 2 cut(s) 49, 55
BstC8I GCNNGC 5 cut(s) 393, 436, 440, 747, 813
BstDEI CTNAG 2 cut(s) 816, 856
BstF5I GGATG 2 cut(s) 654, 765
BstFNI CGCG 2 cut(s) 391, 393
BstH2I RGCGCY 1 cut(s) 834
BstHHI GCGC 4 cut(s) 277, 393, 395, 833
BstKTI GATC 4 cut(s) 256, 292, 304, 325
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 4 cut(s) 253, 289, 301, 322
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 2 cut(s) 445, 768
BstUI CGCG 2 cut(s) 391, 393
BstV1I GCAGC 2 cut(s) 289, 326
BstV2I GAAGAC 1 cut(s) 910
BsuI GTATCC 1 cut(s) 767
BsuRI GGCC 2 cut(s) 543, 707
BtsCI GGATG 2 cut(s) 654, 765
BtsIMutI CAGTG 1 cut(s) 621
Cac8I GCNNGC 5 cut(s) 393, 436, 440, 747, 813
CaiI CAGNNNCTG 1 cut(s) 623
CfoI GCGC 4 cut(s) 277, 393, 395, 833
Cfr13I GGNCC 2 cut(s) 209, 705
Csp6I GTAC 1 cut(s) 787
CviAII CATG 3 cut(s) 396, 425, 738
CviQI GTAC 1 cut(s) 787
DdeI CTNAG 2 cut(s) 816, 856
DpnI GATC 4 cut(s) 255, 291, 303, 324
DpnII GATC 4 cut(s) 253, 289, 301, 322
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 2 cut(s) 49, 55
EarI CTCTTC 2 cut(s) 49, 55
Eco130I CCWWGG 1 cut(s) 544
Eco47I GGWCC 1 cut(s) 209
Eco57I CTGAAG 2 cut(s) 653, 674
Eco88I CYCGRG 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 544
FaeI CATG 3 cut(s) 399, 428, 741
FaiI YATR 8 cut(s) 84, 397, 426, 464, 530, 540, 701, 739
FaqI GGGAC 1 cut(s) 546
FatI CATG 3 cut(s) 395, 424, 737
Fnu4HI GCNGC 2 cut(s) 278, 315
FokI GGATG 2 cut(s) 661, 752
Fsp4HI GCNGC 2 cut(s) 278, 315
FspBI CTAG 2 cut(s) 435, 746
FspI TGCGCA 1 cut(s) 276
GlaI GCGC 4 cut(s) 276, 392, 394, 832
GluI GCNGC 2 cut(s) 278, 315
HaeII RGCGCY 1 cut(s) 834
HaeIII GGCC 2 cut(s) 543, 707
HapII CCGG 4 cut(s) 263, 295, 585, 770
HhaI GCGC 4 cut(s) 277, 393, 395, 833
Hin1II CATG 3 cut(s) 399, 428, 741
Hin6I GCGC 4 cut(s) 275, 391, 393, 831
HinP1I GCGC 4 cut(s) 275, 391, 393, 831
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 4 cut(s) 176, 182, 458, 730
HpaII CCGG 4 cut(s) 263, 295, 585, 770
HphI GGTGA 3 cut(s) 145, 560, 909
Hpy166II GTNNAC 1 cut(s) 551
Hpy188I TCNGA 6 cut(s) 181, 306, 601, 688, 887, 902
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 1 cut(s) 551
Hpy99I CGWCG 2 cut(s) 350, 887
HpyAV CCTTC 4 cut(s) 28, 32, 99, 330
HpyCH4III ACNGT 4 cut(s) 209, 537, 619, 883
HpyCH4V TGCA 4 cut(s) 314, 556, 566, 784
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 2 cut(s) 816, 856
Hsp92II CATG 3 cut(s) 399, 428, 741
HspAI GCGC 4 cut(s) 275, 391, 393, 831
Kzo9I GATC 4 cut(s) 253, 289, 301, 322
LmnI GCTCC 2 cut(s) 447, 742
Lsp1109I GCAGC 2 cut(s) 289, 326
LweI GCATC 3 cut(s) 301, 639, 798
MaeI CTAG 2 cut(s) 435, 746
MaeIII GTNAC 2 cut(s) 619, 915
MalI GATC 4 cut(s) 255, 291, 303, 324
MboI GATC 4 cut(s) 253, 289, 301, 322
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 3 cut(s) 37, 214, 236
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 2 cut(s) 176, 452
MmeI TCCRAC 3 cut(s) 144, 432, 865
Mox20I TGGCCA 1 cut(s) 543
MscI TGGCCA 1 cut(s) 543
MseI TTAA 2 cut(s) 110, 380
Msp20I TGGCCA 1 cut(s) 543
MspI CCGG 4 cut(s) 263, 295, 585, 770
MspR9I CCNGG 2 cut(s) 447, 770
MvaI CCWGG 1 cut(s) 447
MvnI CGCG 2 cut(s) 391, 393
MwoI GCNNNNNNNGC 1 cut(s) 482
NciI CCSGG 1 cut(s) 770
NdeII GATC 4 cut(s) 253, 289, 301, 322
NheI GCTAGC 2 cut(s) 434, 745
NlaIII CATG 3 cut(s) 399, 428, 741
NlaIV GGNNCC 1 cut(s) 706
NmuCI GTSAC 2 cut(s) 619, 915
NsbI TGCGCA 1 cut(s) 276
PaeR7I CTCGAG 1 cut(s) 674
PasI CCCWGGG 1 cut(s) 446
PauI GCGCGC 1 cut(s) 391
PfeI GAWTC 2 cut(s) 176, 730
PfoI TCCNGGA 1 cut(s) 768
PkrI GCNGC 2 cut(s) 279, 316
PleI GAGTC 2 cut(s) 176, 452
PpsI GAGTC 2 cut(s) 176, 452
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 706
PspPI GGNCC 2 cut(s) 209, 705
PspXI VCTCGAGB 1 cut(s) 674
PstNI CAGNNNCTG 1 cut(s) 623
PteI GCGCGC 1 cut(s) 391
RsaI GTAC 1 cut(s) 788
RsaNI GTAC 1 cut(s) 787
SaqAI TTAA 2 cut(s) 110, 380
SatI GCNGC 2 cut(s) 278, 315
Sau3AI GATC 4 cut(s) 253, 289, 301, 322
Sau96I GGNCC 2 cut(s) 209, 705
SchI GAGTC 2 cut(s) 176, 452
ScrFI CCNGG 2 cut(s) 447, 770
SfaNI GCATC 3 cut(s) 301, 639, 798
Sfr274I CTCGAG 1 cut(s) 674
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 674
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 3 cut(s) 37, 214, 236
SsiI CCGC 1 cut(s) 156
SspMI CTAG 2 cut(s) 435, 746
StyD4I CCNGG 2 cut(s) 445, 768
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 4 cut(s) 209, 537, 619, 883
TaqI TCGA 4 cut(s) 300, 345, 614, 675
TasI AATT 3 cut(s) 37, 214, 236
TfiI GAWTC 2 cut(s) 176, 730
Tru1I TTAA 2 cut(s) 110, 380
Tru9I TTAA 2 cut(s) 110, 380
TscAI CASTG 1 cut(s) 628
TseFI GTSAC 2 cut(s) 619, 915
TseI GCWGC 2 cut(s) 277, 314
Tsp45I GTSAC 2 cut(s) 619, 915
TspDTI ATGAA 3 cut(s) 642, 726, 768
TspGWI ACGGA 2 cut(s) 59, 653
TspRI CASTG 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 214
XhoI CTCGAG 1 cut(s) 674
XspI CTAG 2 cut(s) 435, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.