pycom13g00380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010399
Physical Location & Seq
Reverse (-)
246137 .. 247305
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1068 bp
ATGCGGATGAGGCAGATGTGGATGAGATCATTGACATGGCTAATAAAGCCTCCATTGCTCGATCAACAGAAACAAGTCCAGGAAAACATTCATCTCCAAATTAAAAACTTCTGCGTGTCTATGGATGAAGTTCTTCTCCCTGATGTTAAGAAGTTAAATGTGGGAACTGAATCGCCTAAACAATCAAATGCTTCTACTCGACGAAGTGGTCTTAGTTTTGCCATTGGTAGGAATGGTCCACCAGTTAAACATCGTGATGTGCCGGAAACAAGGCCATTACAGCGCACTGATGTTTCTCAAAAGTTAAAAGATCTCTTTGGCTACACGCTTGATATCAAACCTTCTCAAATACCCCACAAGGAAGCAGGCCAAGGTTTATTTCTAAATGGTGAATGTGATGTTGGTGCTGTGGTGGCCGTGTACCCTGGTGTAGTATATTCCCCTGCGTATTACCGCTACATACCTGGATACCCAAGAGTTAATGCTCAAAACTCATATTTGATCACAAGGTATGATGGGACTGTGATCAATGCACAACCTTGGGGTTATGGGGGCGAAACCCGTGAATTTTGGGATGGTTTAACTGTTCCAGAAATCAGGCCTAATATGCATGGAGATGAGAAAGGTCGAGATCGGATCTGGAGACTTCTCAGTAAGCCTTTGGATCAGAGGAAATTGGGAAACAGTGGCGACATAATAGAGCGGAGGAATCCATTAGCTTTGGCTCATTTTGCCAACCATCCTGCAAAGGATATGTCTCCAAATGTTATGGTTTGCCCTTATGACTTCCCGTTGACCGAAAAAGAAATGAGGGTCTATATTCCAAATGTAATATTTGGAGATGCAGAGGAAGTGAAGATGAAGAGATTTGGAAGCTTTTGGTTCAAGCTTGGAGGTTCAAAAAATAGCGGCTCAGATTTTCCTGTTTTGAAGAGTCTTGCCCTGGTGGCTACAAGGGCACTTAGTGATGAAGAAGTGCTTCTGAACTACAGGTTGAGCAACTCTAAGCGTCGGCCAGAGTGGTATACTCCTGTTGATGAGGAGGAAGATCGAAGGAGGTGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

356

Amino Acids

40.73

Weight (kDa)

9.3

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 207
AccBSI CCGCTC 1 cut(s) 703
AccI GTMKAC 1 cut(s) 1025
AciI CCGC 4 cut(s) 4, 454, 703, 909
AclWI GGATC 2 cut(s) 644, 672
AcoI YGGCCR 2 cut(s) 414, 1013
AcsI RAATTY 1 cut(s) 566
AdeI CACNNNGTG 1 cut(s) 965
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 1 cut(s) 228
AgsI TTSAA 3 cut(s) 886, 900, 931
AjnI CCWGG 4 cut(s) 78, 424, 463, 942
AjuI GAANNNNNNNTTGG 4 cut(s) 363, 384, 395, 416
AluBI AGCT 4 cut(s) 719, 876, 889, 1065
AluI AGCT 4 cut(s) 719, 876, 889, 1065
Alw26I GTCTC 2 cut(s) 637, 762
AlwI GGATC 2 cut(s) 644, 672
AoxI GGCC 5 cut(s) 272, 367, 414, 599, 1013
ApoI RAATTY 1 cut(s) 566
ArsI GACNNNNNNTTYG 2 cut(s) 740, 772
Asp700I GAANNNNTTC 3 cut(s) 87, 132, 978
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 1 cut(s) 236
AsuHPI GGTGA 1 cut(s) 401
AvaII GGWCC 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 961
BccI CCATC 3 cut(s) 509, 569, 747
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 4 cut(s) 80, 426, 465, 944
BciVI GTATCC 1 cut(s) 461
BclI TGATCA 2 cut(s) 501, 525
BcoDI GTCTC 2 cut(s) 637, 762
BfmI CTRYAG 1 cut(s) 988
BfuI GTATCC 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 947
BglII AGATCT 1 cut(s) 310
BisI GCNGC 1 cut(s) 910
BlsI GCNGC 1 cut(s) 911
Bme1390I CCNGG 4 cut(s) 80, 426, 465, 944
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 236
BmrFI CCNGG 4 cut(s) 80, 426, 465, 944
BmsI GCATC 1 cut(s) 832
BpmI CTGGAG 1 cut(s) 661
BsaJI CCNNGG 4 cut(s) 370, 424, 539, 942
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse1I ACTGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 53
BseBI CCWGG 4 cut(s) 80, 426, 465, 944
BseDI CCNNGG 4 cut(s) 370, 424, 539, 942
BseGI GGATG 5 cut(s) 12, 27, 130, 580, 739
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMI GCAATG 1 cut(s) 53
BseMII CTCAG 2 cut(s) 664, 927
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 1055
BseSI GKGCMC 1 cut(s) 961
BshFI GGCC 5 cut(s) 274, 369, 416, 601, 1015
BsiSI CCGG 1 cut(s) 263
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 2 cut(s) 637, 762
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 5 cut(s) 274, 369, 416, 601, 1015
Bsp1286I GDGCHC 1 cut(s) 961
Bsp143I GATC 9 cut(s) 26, 61, 310, 501, 525, 631, 636, 664, 1048
BspACI CCGC 4 cut(s) 4, 454, 703, 909
BspANI GGCC 5 cut(s) 274, 369, 416, 601, 1015
BspCNI CTCAG 2 cut(s) 663, 926
BspPI GGATC 2 cut(s) 644, 672
BsrBI CCGCTC 1 cut(s) 703
BsrDI GCAATG 1 cut(s) 53
BsrI ACTGG 1 cut(s) 242
BssECI CCNNGG 4 cut(s) 370, 424, 539, 942
BssMI GATC 9 cut(s) 26, 61, 310, 501, 525, 631, 636, 664, 1048
BssNAI GTATAC 1 cut(s) 1026
BssT1I CCWWGG 2 cut(s) 370, 539
Bst1107I GTATAC 1 cut(s) 1026
Bst2UI CCWGG 4 cut(s) 80, 426, 465, 944
Bst4CI ACNGT 3 cut(s) 523, 586, 686
Bst6I CTCTTC 2 cut(s) 857, 926
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 5 cut(s) 212, 650, 913, 962, 1005
BstF5I GGATG 5 cut(s) 12, 27, 130, 580, 739
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 9 cut(s) 29, 64, 313, 504, 528, 634, 639, 667, 1051
BstMAI GTCTC 2 cut(s) 637, 762
BstMBI GATC 9 cut(s) 26, 61, 310, 501, 525, 631, 636, 664, 1048
BstMWI GCNNNNNNNGC 9 cut(s) 10, 46, 55, 280, 413, 607, 731, 947, 956
BstNI CCWGG 4 cut(s) 80, 426, 465, 944
BstSCI CCNGG 4 cut(s) 78, 424, 463, 942
BstSFI CTRYAG 1 cut(s) 988
BstSLI GKGCMC 1 cut(s) 961
BstX2I RGATCY 2 cut(s) 310, 636
BstYI RGATCY 2 cut(s) 310, 636
BstZ17I GTATAC 1 cut(s) 1026
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 5 cut(s) 274, 369, 416, 601, 1015
BtsCI GGATG 5 cut(s) 12, 27, 130, 580, 739
BtsIMutI CAGTG 2 cut(s) 285, 691
Cac8I GCNNGC 1 cut(s) 367
CfoI GCGC 1 cut(s) 285
Cfr13I GGNCC 1 cut(s) 236
CseI GACGC 1 cut(s) 998
Csp6I GTAC 1 cut(s) 421
CviAII CATG 2 cut(s) 36, 611
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 5 cut(s) 212, 650, 913, 962, 1005
DpnI GATC 9 cut(s) 28, 63, 312, 503, 527, 633, 638, 666, 1050
DpnII GATC 9 cut(s) 26, 61, 310, 501, 525, 631, 636, 664, 1048
DraIII CACNNNGTG 1 cut(s) 965
DrdI GACNNNNNNGTC 1 cut(s) 207
DseDI GACNNNNNNGTC 1 cut(s) 207
EaeI YGGCCR 2 cut(s) 414, 1013
Eam1104I CTCTTC 2 cut(s) 857, 926
EarI CTCTTC 2 cut(s) 857, 926
Eco130I CCWWGG 2 cut(s) 370, 539
Eco147I AGGCCT 1 cut(s) 601
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 236
EcoRII CCWGG 4 cut(s) 78, 424, 463, 942
EcoRV GATATC 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 370, 539
EcoT22I ATGCAT 1 cut(s) 612
ErhI CCWWGG 2 cut(s) 370, 539
FaeI CATG 2 cut(s) 39, 614
FalI AAGNNNNNCTT 2 cut(s) 963, 995
FaqI GGGAC 1 cut(s) 532
FatI CATG 2 cut(s) 35, 610
FbaI TGATCA 2 cut(s) 501, 525
FblI GTMKAC 1 cut(s) 1025
Fnu4HI GCNGC 1 cut(s) 910
FokI GGATG 5 cut(s) 19, 34, 137, 587, 726
Fsp4HI GCNGC 1 cut(s) 910
GlaI GCGC 1 cut(s) 284
GluI GCNGC 1 cut(s) 910
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 5 cut(s) 274, 369, 416, 601, 1015
HapII CCGG 1 cut(s) 263
HgaI GACGC 1 cut(s) 998
HhaI GCGC 1 cut(s) 285
Hin1II CATG 2 cut(s) 39, 614
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 2 cut(s) 874, 887
HinfI GANTC 3 cut(s) 170, 709, 934
HpaII CCGG 1 cut(s) 263
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 4 cut(s) 239, 421, 795, 1026
Hpy188I TCNGA 4 cut(s) 636, 669, 916, 984
Hpy188III TCNNGA 4 cut(s) 254, 590, 629, 640
Hpy8I GTNNAC 4 cut(s) 239, 421, 795, 1026
Hpy99I CGWCG 2 cut(s) 204, 1014
HpyAV CCTTC 2 cut(s) 351, 1047
HpyCH4III ACNGT 3 cut(s) 523, 586, 686
HpyCH4V TGCA 4 cut(s) 533, 610, 746, 845
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 46, 55, 280, 413, 607, 731, 947, 956
HpyF3I CTNAG 5 cut(s) 212, 650, 913, 962, 1005
Hsp92II CATG 2 cut(s) 39, 614
HspAI GCGC 1 cut(s) 283
Ksp22I TGATCA 2 cut(s) 501, 525
Kzo9I GATC 9 cut(s) 26, 61, 310, 501, 525, 631, 636, 664, 1048
LmnI GCTCC 1 cut(s) 1062
LweI GCATC 1 cut(s) 832
MalI GATC 9 cut(s) 28, 63, 312, 503, 527, 633, 638, 666, 1050
MbiI CCGCTC 1 cut(s) 703
MboI GATC 9 cut(s) 26, 61, 310, 501, 525, 631, 636, 664, 1048
MboII GAAGA 6 cut(s) 125, 868, 874, 943, 983, 1058
MflI RGATCY 2 cut(s) 310, 636
MhlI GDGCHC 1 cut(s) 961
MluCI AATT 3 cut(s) 99, 566, 674
MlyI GAGTC 1 cut(s) 943
Mph1103I ATGCAT 1 cut(s) 612
MroXI GAANNNNTTC 3 cut(s) 87, 132, 978
MseI TTAA 7 cut(s) 102, 147, 155, 246, 305, 480, 581
MslI CAYNNNNRTG 3 cut(s) 34, 255, 615
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 4 cut(s) 80, 426, 465, 944
MvaI CCWGG 4 cut(s) 80, 426, 465, 944
MwoI GCNNNNNNNGC 9 cut(s) 10, 46, 55, 280, 413, 607, 731, 947, 956
NdeII GATC 9 cut(s) 26, 61, 310, 501, 525, 631, 636, 664, 1048
NlaIII CATG 2 cut(s) 39, 614
NsiI ATGCAT 1 cut(s) 612
PceI AGGCCT 1 cut(s) 601
PdmI GAANNNNTTC 3 cut(s) 87, 132, 978
PfeI GAWTC 2 cut(s) 170, 709
PfoI TCCNGGA 1 cut(s) 78
PkrI GCNGC 1 cut(s) 911
PleI GAGTC 1 cut(s) 942
PpsI GAGTC 1 cut(s) 942
Psp6I CCWGG 4 cut(s) 78, 424, 463, 942
PspGI CCWGG 4 cut(s) 78, 424, 463, 942
PspPI GGNCC 1 cut(s) 236
PsuI RGATCY 2 cut(s) 310, 636
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
RseI CAYNNNNRTG 3 cut(s) 34, 255, 615
SaqAI TTAA 7 cut(s) 102, 147, 155, 246, 305, 480, 581
SatI GCNGC 1 cut(s) 910
Sau3AI GATC 9 cut(s) 26, 61, 310, 501, 525, 631, 636, 664, 1048
Sau96I GGNCC 1 cut(s) 236
SchI GAGTC 1 cut(s) 943
ScrFI CCNGG 4 cut(s) 80, 426, 465, 944
SduI GDGCHC 1 cut(s) 961
SfaNI GCATC 1 cut(s) 832
SfcI CTRYAG 1 cut(s) 988
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 3 cut(s) 34, 255, 615
Sse9I AATT 3 cut(s) 99, 566, 674
SseBI AGGCCT 1 cut(s) 601
SsiI CCGC 4 cut(s) 4, 454, 703, 909
SspI AATATT 1 cut(s) 834
StuI AGGCCT 1 cut(s) 601
StyD4I CCNGG 4 cut(s) 78, 424, 463, 942
StyI CCWWGG 2 cut(s) 370, 539
TaaI ACNGT 3 cut(s) 523, 586, 686
TaqI TCGA 4 cut(s) 60, 199, 628, 1051
TaqII GACCGA 1 cut(s) 812
TasI AATT 3 cut(s) 99, 566, 674
TauI GCSGC 1 cut(s) 912
TfiI GAWTC 2 cut(s) 170, 709
Tru1I TTAA 7 cut(s) 102, 147, 155, 246, 305, 480, 581
Tru9I TTAA 7 cut(s) 102, 147, 155, 246, 305, 480, 581
TscAI CASTG 2 cut(s) 292, 691
TspDTI ATGAA 4 cut(s) 80, 141, 875, 984
TspRI CASTG 2 cut(s) 292, 691
VpaK11BI GGWCC 1 cut(s) 236
XapI RAATTY 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 1025
XmnI GAANNNNTTC 3 cut(s) 87, 132, 978
Zsp2I ATGCAT 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.