Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0002956

Physical Location & Seq

Genomic Coordinates Forward (+)

413952 .. 420211

Gene Structure 6260 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2616 bp

ATGGTTCAGGCCCCAGTCCATGGCTTGTCGTCTTCTGCCAGTCCTTTTTCATACAACATTCCAAAATCTGGTTATTCTTTCCCTAATAGCCAGCAGTTTCAGTCTGGCACGAATATCACACCTGCTACTGCACAAGAAACTGGTAATGCCTCATTGTCCTCAACTAGTTTGCATTCTGGTTCCTTGCCTGCTCCTACTTCAAGTTCCACAGTGAATATATCATCAGCTCCTAATGCGGGACCAAAAGCTTCATGGATTCCCACTGCCCCATCTTTCAACATGACTCCCGGAATGCCTGGAACTCCCAGGACACCTGGCCTCCCTGGAATAGCTCACTCAGTGCAAATATCTTTTAATCCGACTGCACCGTCTGCACCTATTGATTCTTCTTCTGTAGCCCTTAGACCTAACATGCAAGCAGTACCTGTTGCGTCATCAGCAGTTCAGCCACATGTAGGTGCTCCGTACCCATCTTTATCTGCTATGGGTGCTCCCTGGCTTCCGTCCCCACAGATAGGAGGCTTACCTAGGCCAACGTTTTTGCCATATCCTGCTGCCTTTCCTGGTCCCTTTCCTTTGCCTGCACATGTTATGCCTCTCTCTTCTGTTCCTTTACCTGATTCTCAGCCTCCTGGTGTCACTCCTGTGGGAAATACTGTAGCAAATTCTTTGTCTTCCGTTGGTTCTGGTCATCAATTGGCAGGGTCTTCAGGGATGCAGAAAGAATTACCTCATCCTGGCGTTGGTACCGATAGTAGAGCTGTTGTCAATGAACAATTGGATGCCTGGACTGCCCACAAGACAGAGACAGGTGTTGTATACTATTATAATGCTTTGACCGGTGAATCAACTTATGATAAGCCTCCTGGATTTAGGGAGGAGCCTGGTAAGGTTACTATGCAGCCAACTCCAGTTTCAACGGTGAATTTGACTGGAACTGATTGGGTATTGGTCACCACCAGTGATGGAAAAAAGTTTTACCATAACAGCAAAACCAAGGTAAGTAGCTGGCAGATCCCAAATGAAGTGATTGAATTGAGAAATAAGCAGGATAGTGACGTGCCAAAAGTACATACAATATCAGTGCCAAATAATAATTTAATGATTGAAAAGGGATCTGCTCCAGTTAGTTTGAGTGCTCCAGCCATTAACACGGGTGGCCGGGAAGCCATGCCTTTTAAACCCTCAGCTGTGCAGGGAACATCTTCTGCACTTGATCTGATTAAAAGGAAGTTGCAGGATCCTGTTACATCTTCACCAATTTCAGCACCATCAGAATCAAATGGTGCAAGAGGAGTTGAGAGTACACCTAAGGGCCAGCAAAGCGAGAATAGCAAAGATAAGCTGAAAGACACTAATGGAGATGGAAACTTGTCTGATTCCTCATCAGATTCAGAGGATGCAGACAGTGGGCCAACAAAGGAGGAGTGTATTATTCAATTCAAGGAGATGCTCAAAGAACGGGGAGTGGCACCATTCTCGAAGTGGGAGAAGGAACTGCCAAAGATAGTGTTTGATCCACGCTTTAAGGCCATCCCAAGTCATGAAGCTCGCAGGTCCTTGTTTGAGCACTATGTTAAAACACGTGCAGAGGAGGAGAGAAAGGAAAAAAGAGCAGCTCAGAAGGCTGCAATAGAGGGATTTAAGCAGTTATTGGATGAAGCATCGGAGGATATTGACCATAACACTGATTACCAAGGTTTCAGAAAGAAATGGGCCAATGATCCACGCTTTGAGGCTTTAGATCGCAAAGATCGGGAGCATTTGTTGAATGAAAGGGTCCTTCCATTGAAGAGGGCTACTGAAGAAAAGGCTCAAGCAGAGCGTGCTGCTGCTGCAGCTGGTTTTAAGTCCATGCTTAAAGAGAAAGGAGATATTACTGTTAGTTCTCGTTGGTCCAGGGTGAAAGATAGTCTGCGGAATGATCCGAGGTACAAAAATGTTAGGCATGAGGATAGGGAAGTTTTATTCAATGAGCATATATCGGGACTAAAAGCTGTGGAAGAGGAAGCAGAACAAGAGGCAAAAGCTAAACGTGACGAGCAGGAAAAACTGAGGGAAAGGGAACGAGAACTGCGGAAGCGGAAGGAAAGAGAAGAGCAAGAAACAGAGAGGGTGCGACTGAAAGTTCGCAGGAAGGAGGCAGTTGCTACTTTTCAAGCTCTACTTGTGGAAACAATTAAAGATCCTCAGGCATCTTGGACAGGTTCTAAGCCTAAACTGGAGAAGGATCCACAAAGGCGTGCAGCCAACCCTGATTTAGATCCATCTGACATGGAAAAGCTCTTCTGGGAACATGTAAAGATGCTAAACGAGCGATGTGCACATGAGTTCAGAACTCTGTTGGCCGAAGTCTTGACTGCAGAAGCAGCGTCCCAAGAGACAGAAGATGGAAAAACAGTTCTTAATTCGTGGTCAACAGCTAAACGTATTCTAAAGATGGATCCCAGATACGACAAGATGCCAAGAAAAGAGAGGGAAGTTTTGTGGAGACGATATTCTGAGGAGATGCTGCGGAAGCAAAAGTCAGATAGGAAAACAGATGCTAAAAGTAGAAGTTCTATTGACGCTGGGAGGAATCCCTACGGATCTAGGGGTACTCACGATCGGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0001085 GO:0001103 GO:0003674 GO:0003712 GO:0003713 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0070491 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03040 ko04212 map03040 map04212

Pfam Domains

Protein Families

FF WW

Protein Analysis

872

Amino Acids

96.0

Weight (kDa)

8.98

Isoelectric Point (pI)

53.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	262 - 288	5.9e-08	WW domain
WW_TCERG1	PF23517	302 - 345	2e-09	TCERG1 WW domain
WW	PF00397	313 - 340	1.4e-06	WW domain
FF	PF01846	480 - 525	5.7e-14	FF domain
FF	PF01846	544 - 592	2.1e-08	FF domain
FF	PF01846	614 - 660	5.5e-13	FF domain
FF	PF01846	714 - 766	3.2e-06	FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002956)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19840
fragaria_vesca	FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700
malus_domestica	MD13G1007900.v1.1 MD13G1008000.v1.1 MD16G1000200.v1.1
prunus_persica	Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1
pyrus_communis	pycom13g00650 pycom16g00500 pycom16g00510 pycom16g00520
rosa_chinensis	RchiOBHm_Chr4g0446421
rosa_laevigata	RLG00000005662
rosa_multiflora	Rmu_co8175444.1_g000001 Rmu_sc0008360.1_g000005 Rmu_sc0018208.1_g000001 Rmu_sc0025430.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386860
rosa_rugosa	Rorug04G0371200
rosa_samantha	Rh4AG432800 Rh4BG441900 Rh4CG459300 Rh4DG440700
rosa_wichuraiana	Rw4G036970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	828
AarI	CACCTGC	1 cut(s)	130
AasI	GACNNNNNNGTC	1 cut(s)	367
Acc36I	ACCTGC	2 cut(s)	130, 1545
Acc65I	GGTACC	1 cut(s)	746
AccB1I	GGYRCC	2 cut(s)	746, 1471
AccB7I	CCANNNNNTGG	2 cut(s)	20, 68
AccI	GTMKAC	1 cut(s)	819
AciI	CCGC	5 cut(s)	236, 1918, 2077, 2083, 2515
AclI	AACGTT	1 cut(s)	536
AcoI	YGGCCR	2 cut(s)	1159, 2346
AcsI	RAATTY	2 cut(s)	664, 925
AcuI	CTGAAG	2 cut(s)	693, 1824
AcvI	CACGTG	1 cut(s)	1586
AdeI	CACNNNGTG	1 cut(s)	340
AfaI	GTAC	7 cut(s)	423, 467, 748, 1071, 1306, 1934, 2599
AfiI	CCNNNNNNNGG	7 cut(s)	20, 68, 236, 455, 515, 737, 743
AflIII	ACRYGT	4 cut(s)	451, 586, 1583, 2296
AgeI	ACCGGT	1 cut(s)	839
AhlI	ACTAGT	1 cut(s)	164
AjiI	CACGTC	1 cut(s)	1060
AjuI	GAANNNNNNNTTGG	2 cut(s)	898, 930
AleI	CACNNNNGTG	1 cut(s)	644
AloI	GAACNNNNNNTCC	2 cut(s)	2385, 2417
Alw21I	GWGCWC	5 cut(s)	463, 493, 1141, 1572, 2326
Alw26I	GTCTC	3 cut(s)	800, 2375, 2485
Alw44I	GTGCAC	1 cut(s)	2322
AlwNI	CAGNNNCTG	1 cut(s)	425
AoxI	GGCC	9 cut(s)	9, 316, 530, 1159, 1315, 1412, 1530, 1716, 2346
ApaLI	GTGCAC	1 cut(s)	2322
ApoI	RAATTY	2 cut(s)	664, 925
ArsI	GACNNNNNNTTYG	4 cut(s)	656, 688, 2112, 2144
AsiGI	ACCGGT	1 cut(s)	839
Asp700I	GAANNNNTTC	1 cut(s)	1204
Asp718I	GGTACC	1 cut(s)	746
AspA2I	CCTAGG	1 cut(s)	527
AspS9I	GGNCC	9 cut(s)	10, 239, 566, 1315, 1412, 1557, 1716, 1780, 1896
AsuC2I	CCSGG	2 cut(s)	288, 1163
AsuHPI	GGTGA	5 cut(s)	854, 934, 946, 1248, 1915
AvaII	GGWCC	5 cut(s)	239, 566, 1557, 1780, 1896
AvrII	CCTAGG	1 cut(s)	527
AxyI	CCTNAGG	2 cut(s)	1311, 2190
BaeGI	GKGCMC	1 cut(s)	2326
BamHI	GGATCC	3 cut(s)	1240, 2230, 2443
BanI	GGYRCC	2 cut(s)	746, 1471
BbrPI	CACGTG	1 cut(s)	1586
BbsI	GAAGAC	3 cut(s)	24, 666, 699
Bbv12I	GWGCWC	5 cut(s)	463, 493, 1141, 1572, 2326
BbvCI	CCTCAGC	1 cut(s)	1186
BccI	CCATC	9 cut(s)	277, 478, 959, 1279, 1358, 1541, 2275, 2384, 2434
BcgI	CGANNNNNNTGC	4 cut(s)	1461, 1495, 2303, 2337
BcnI	CCSGG	2 cut(s)	288, 1163
BcoDI	GTCTC	3 cut(s)	800, 2375, 2485
BcuI	ACTAGT	1 cut(s)	164
BfaI	CTAG	3 cut(s)	165, 528, 2592
BfmI	CTRYAG	4 cut(s)	393, 657, 1836, 2361
BfuAI	ACCTGC	2 cut(s)	130, 1545
BlnI	CCTAGG	1 cut(s)	527
Bme18I	GGWCC	5 cut(s)	239, 566, 1557, 1780, 1896
BmgBI	CACGTC	1 cut(s)	1060
BmgT120I	GGNCC	9 cut(s)	10, 239, 566, 1315, 1412, 1557, 1716, 1780, 1896
BmrI	ACTGGG	1 cut(s)	8
BmuI	ACTGGG	1 cut(s)	8
BpiI	GAAGAC	3 cut(s)	24, 666, 699
BpmI	CTGGAG	4 cut(s)	894, 1107, 1125, 2243
Bpu10I	CCTNAGC	1 cut(s)	1186
BpuEI	CTTGAG	1 cut(s)	1800
BpuMI	CCSGG	2 cut(s)	288, 1163
BsaAI	YACGTR	1 cut(s)	1586
BsaBI	GATNNNNATC	1 cut(s)	2262
BsaJI	CCNNGG	9 cut(s)	19, 305, 322, 494, 527, 996, 1696, 1899, 1928
BsaWI	WCCGGW	1 cut(s)	839
BsaXI	ACNNNNNCTCC	4 cut(s)	303, 333, 1751, 1781
Bsc4I	CCNNNNNNNGG	7 cut(s)	20, 68, 236, 455, 515, 737, 743
Bse118I	RCCGGY	1 cut(s)	839
Bse1I	ACTGG	8 cut(s)	14, 39, 145, 911, 937, 960, 1124, 2226
Bse21I	CCTNAGG	2 cut(s)	1311, 2190
Bse8I	GATNNNNATC	1 cut(s)	2262
BseDI	CCNNGG	9 cut(s)	19, 305, 322, 494, 527, 996, 1696, 1899, 1928
BseGI	GGATG	6 cut(s)	720, 733, 787, 1405, 1533, 1663
BseJI	GATNNNNATC	1 cut(s)	2262
BseLI	CCNNNNNNNGG	7 cut(s)	20, 68, 236, 455, 515, 737, 743
BseMII	CTCAG	7 cut(s)	351, 638, 1200, 1634, 2045, 2204, 2493
BseNI	ACTGG	8 cut(s)	14, 39, 145, 911, 937, 960, 1124, 2226
BseRI	GAGGAG	6 cut(s)	893, 1308, 1439, 1607, 1610, 2519
BseSI	GKGCMC	1 cut(s)	2326
BseYI	CCCAGC	1 cut(s)	2570
BsgI	GTGCAG	8 cut(s)	114, 348, 357, 567, 1194, 1214, 1608, 2265
Bsh1285I	CGRYCG	1 cut(s)	2608
BshFI	GGCC	9 cut(s)	11, 318, 532, 1161, 1317, 1414, 1532, 1718, 2348
BshNI	GGYRCC	2 cut(s)	746, 1471
BshTI	ACCGGT	1 cut(s)	839
BsiEI	CGRYCG	1 cut(s)	2608
BsiHKAI	GWGCWC	5 cut(s)	463, 493, 1141, 1572, 2326
BsiSI	CCGG	3 cut(s)	288, 840, 1162
BslFI	GGGAC	5 cut(s)	252, 490, 552, 2001, 2359
BslI	CCNNNNNNNGG	7 cut(s)	20, 68, 236, 455, 515, 737, 743
BsmAI	GTCTC	3 cut(s)	800, 2375, 2485
BsmBI	CGTCTC	1 cut(s)	2485
BsmFI	GGGAC	5 cut(s)	252, 490, 552, 2001, 2359
BsmI	GAATGC	2 cut(s)	172, 297
BsnI	GGCC	9 cut(s)	11, 318, 532, 1161, 1317, 1414, 1532, 1718, 2348
Bsp1286I	GDGCHC	5 cut(s)	463, 493, 1141, 1572, 2326
Bsp19I	CCATGG	1 cut(s)	19
BspACI	CCGC	5 cut(s)	236, 1918, 2077, 2083, 2515
BspANI	GGCC	9 cut(s)	11, 318, 532, 1161, 1317, 1414, 1532, 1718, 2348
BspCNI	CTCAG	7 cut(s)	350, 637, 1199, 1633, 2046, 2203, 2494
BspHI	TCATGA	1 cut(s)	1543
BspMAI	CTGCAG	2 cut(s)	1840, 2365
BspMI	ACCTGC	2 cut(s)	130, 1545
BspQI	GCTCTTC	2 cut(s)	2091, 2291
BspT107I	GGYRCC	2 cut(s)	746, 1471
BsrFI	RCCGGY	1 cut(s)	839
BsrI	ACTGG	8 cut(s)	14, 39, 145, 911, 937, 960, 1124, 2226
BssAI	RCCGGY	1 cut(s)	839
BssECI	CCNNGG	9 cut(s)	19, 305, 322, 494, 527, 996, 1696, 1899, 1928
BssNAI	GTATAC	1 cut(s)	820
BssT1I	CCWWGG	4 cut(s)	19, 527, 996, 1696
Bst1107I	GTATAC	1 cut(s)	820
Bst4CI	ACNGT	7 cut(s)	211, 369, 658, 922, 1409, 1882, 2401
Bst6I	CTCTTC	5 cut(s)	607, 1787, 1998, 2091, 2291
BstAPI	GCANNNNNTGC	2 cut(s)	371, 1826
BstBAI	YACGTR	1 cut(s)	1586
BstC8I	GCNNGC	9 cut(s)	92, 189, 417, 582, 1010, 1319, 1552, 1827, 2244
BstDSI	CCRYGG	1 cut(s)	19
BstEII	GGTNACC	1 cut(s)	952
BstF5I	GGATG	6 cut(s)	720, 733, 787, 1405, 1533, 1663
BstMAI	GTCTC	3 cut(s)	800, 2375, 2485
BstMCI	CGRYCG	1 cut(s)	2608
BstNSI	RCATGY	4 cut(s)	415, 455, 590, 2300
BstPI	GGTNACC	1 cut(s)	952
BstSFI	CTRYAG	4 cut(s)	393, 657, 1836, 2361
BstSLI	GKGCMC	1 cut(s)	2326
BstV2I	GAAGAC	3 cut(s)	24, 666, 699
BstX2I	RGATCY	8 cut(s)	1014, 1115, 1240, 2185, 2230, 2263, 2443, 2588
BstYI	RGATCY	8 cut(s)	1014, 1115, 1240, 2185, 2230, 2263, 2443, 2588
BstZ17I	GTATAC	1 cut(s)	820
Bsu36I	CCTNAGG	2 cut(s)	1311, 2190
BsuRI	GGCC	9 cut(s)	11, 318, 532, 1161, 1317, 1414, 1532, 1718, 2348
BtgI	CCRYGG	1 cut(s)	19
BtgZI	GCGATG	1 cut(s)	2332
BtrI	CACGTC	1 cut(s)	1060
BtsCI	GGATG	6 cut(s)	720, 733, 787, 1405, 1533, 1663
BtsI	GCAGTG	1 cut(s)	261
BtsIMutI	CAGTG	7 cut(s)	216, 261, 345, 967, 1089, 1414, 1686
BveI	ACCTGC	2 cut(s)	130, 1545
Cac8I	GCNNGC	9 cut(s)	92, 189, 417, 582, 1010, 1319, 1552, 1827, 2244
CaiI	CAGNNNCTG	1 cut(s)	425
CciI	TCATGA	1 cut(s)	1543
Cfr10I	RCCGGY	1 cut(s)	839
Cfr13I	GGNCC	9 cut(s)	10, 239, 566, 1315, 1412, 1557, 1716, 1780, 1896
CseI	GACGC	3 cut(s)	420, 2361, 2576
Csp6I	GTAC	7 cut(s)	422, 466, 747, 1070, 1305, 1933, 2598
CspAI	ACCGGT	1 cut(s)	839
CviQI	GTAC	7 cut(s)	422, 466, 747, 1070, 1305, 1933, 2598
DraI	TTTAAA	1 cut(s)	1180
DraIII	CACNNNGTG	1 cut(s)	340
DrdI	GACNNNNNNGTC	1 cut(s)	367
DseDI	GACNNNNNNGTC	1 cut(s)	367
EaeI	YGGCCR	2 cut(s)	1159, 2346
Eam1104I	CTCTTC	5 cut(s)	607, 1787, 1998, 2091, 2291
EarI	CTCTTC	5 cut(s)	607, 1787, 1998, 2091, 2291
Eco130I	CCWWGG	4 cut(s)	19, 527, 996, 1696
Eco47I	GGWCC	5 cut(s)	239, 566, 1557, 1780, 1896
Eco57I	CTGAAG	2 cut(s)	693, 1824
Eco72I	CACGTG	1 cut(s)	1586
Eco81I	CCTNAGG	2 cut(s)	1311, 2190
Eco91I	GGTNACC	1 cut(s)	952
EcoO109I	RGGNCCY	3 cut(s)	10, 1557, 1780
EcoO65I	GGTNACC	1 cut(s)	952
EcoT14I	CCWWGG	4 cut(s)	19, 527, 996, 1696
ErhI	CCWWGG	4 cut(s)	19, 527, 996, 1696
Esp3I	CGTCTC	1 cut(s)	2485
FalI	AAGNNNNNCTT	2 cut(s)	2151, 2183
FaqI	GGGAC	5 cut(s)	252, 490, 552, 2001, 2359
FauI	CCCGC	1 cut(s)	229
FblI	GTMKAC	1 cut(s)	819
FokI	GGATG	6 cut(s)	720, 727, 794, 1412, 1520, 1670
FspBI	CTAG	3 cut(s)	165, 528, 2592
GsaI	CCCAGC	1 cut(s)	2574
GsuI	CTGGAG	4 cut(s)	894, 1107, 1125, 2243
HaeIII	GGCC	9 cut(s)	11, 318, 532, 1161, 1317, 1414, 1532, 1718, 2348
HapII	CCGG	3 cut(s)	288, 840, 1162
HgaI	GACGC	3 cut(s)	420, 2361, 2576
HincII	GTYRAC	1 cut(s)	2418
HindII	GTYRAC	1 cut(s)	2418
HindIII	AAGCTT	1 cut(s)	246
HinfI	GANTC	9 cut(s)	256, 283, 383, 620, 845, 1277, 1379, 1391, 2578
HpaII	CCGG	3 cut(s)	288, 840, 1162
HphI	GGTGA	5 cut(s)	854, 934, 946, 1248, 1915
Hpy166II	GTNNAC	4 cut(s)	820, 1307, 2324, 2418
Hpy188III	TCNNGA	6 cut(s)	1480, 1544, 1757, 1986, 2356, 2603
Hpy8I	GTNNAC	4 cut(s)	820, 1307, 2324, 2418
HpyAV	CCTTC	6 cut(s)	1486, 1618, 1793, 2080, 2131, 2221
HpyCH4III	ACNGT	7 cut(s)	211, 369, 658, 922, 1409, 1882, 2401
HpyCH4IV	ACGT	5 cut(s)	536, 1059, 1585, 2035, 2428
HpySE526I	ACGT	5 cut(s)	536, 1059, 1585, 2035, 2428
KpnI	GGTACC	1 cut(s)	750
LguI	GCTCTTC	2 cut(s)	2091, 2291
LmnI	GCTCC	8 cut(s)	196, 232, 466, 496, 880, 1126, 1144, 1759
MaeI	CTAG	3 cut(s)	165, 528, 2592
MaeII	ACGT	5 cut(s)	536, 1059, 1585, 2035, 2428
MaeIII	GTNAC	6 cut(s)	637, 892, 952, 1055, 1246, 2036
MfeI	CAATTG	2 cut(s)	695, 776
MflI	RGATCY	8 cut(s)	1014, 1115, 1240, 2185, 2230, 2263, 2443, 2588
MhlI	GDGCHC	5 cut(s)	463, 493, 1141, 1572, 2326
MlyI	GAGTC	1 cut(s)	277
MmeI	TCCRAC	1 cut(s)	383
MroXI	GAANNNNTTC	1 cut(s)	1204
MslI	CAYNNNNRTG	2 cut(s)	456, 644
MspA1I	CMGCKG	2 cut(s)	1190, 1841
MspI	CCGG	3 cut(s)	288, 840, 1162
MunI	CAATTG	2 cut(s)	695, 776
Mva1269I	GAATGC	2 cut(s)	172, 297
NciI	CCSGG	2 cut(s)	288, 1163
NcoI	CCATGG	1 cut(s)	19
NmuCI	GTSAC	4 cut(s)	637, 952, 1055, 2036
NspI	RCATGY	4 cut(s)	415, 455, 590, 2300
OliI	CACNNNNGTG	1 cut(s)	644
PagI	TCATGA	1 cut(s)	1543
PaqCI	CACCTGC	1 cut(s)	130
PciI	ACATGT	3 cut(s)	451, 586, 2296
PciSI	GCTCTTC	2 cut(s)	2091, 2291
PctI	GAATGC	2 cut(s)	172, 297
PdmI	GAANNNNTTC	1 cut(s)	1204
PfeI	GAWTC	8 cut(s)	256, 383, 620, 845, 1277, 1379, 1391, 2578
PflMI	CCANNNNNTGG	2 cut(s)	20, 68
PfoI	TCCNGGA	2 cut(s)	286, 865
PinAI	ACCGGT	1 cut(s)	839
Ple19I	CGATCG	1 cut(s)	2608
PleI	GAGTC	1 cut(s)	277
PmaCI	CACGTG	1 cut(s)	1586
PmlI	CACGTG	1 cut(s)	1586
PpsI	GAGTC	1 cut(s)	277
Ppu21I	YACGTR	1 cut(s)	1586
PpuMI	RGGWCCY	2 cut(s)	1557, 1780
PscI	ACATGT	3 cut(s)	451, 586, 2296
PsiI	TTATAA	1 cut(s)	828
Psp1406I	AACGTT	1 cut(s)	536
Psp5II	RGGWCCY	2 cut(s)	1557, 1780
PspCI	CACGTG	1 cut(s)	1586
PspEI	GGTNACC	1 cut(s)	952
PspFI	CCCAGC	1 cut(s)	2570
PspPI	GGNCC	9 cut(s)	10, 239, 566, 1315, 1412, 1557, 1716, 1780, 1896
PspPPI	RGGWCCY	2 cut(s)	1557, 1780
PstI	CTGCAG	2 cut(s)	1840, 2365
PstNI	CAGNNNCTG	1 cut(s)	425
PsuI	RGATCY	8 cut(s)	1014, 1115, 1240, 2185, 2230, 2263, 2443, 2588
PvuI	CGATCG	1 cut(s)	2608
PvuII	CAGCTG	2 cut(s)	1190, 1841
RsaI	GTAC	7 cut(s)	423, 467, 748, 1071, 1306, 1934, 2599
RsaNI	GTAC	7 cut(s)	422, 466, 747, 1070, 1305, 1933, 2598
RseI	CAYNNNNRTG	2 cut(s)	456, 644
SapI	GCTCTTC	2 cut(s)	2091, 2291
Sau96I	GGNCC	9 cut(s)	10, 239, 566, 1315, 1412, 1557, 1716, 1780, 1896
SchI	GAGTC	1 cut(s)	277
SduI	GDGCHC	5 cut(s)	463, 493, 1141, 1572, 2326
SfcI	CTRYAG	4 cut(s)	393, 657, 1836, 2361
SinI	GGWCC	5 cut(s)	239, 566, 1557, 1780, 1896
SmiMI	CAYNNNNRTG	2 cut(s)	456, 644
SmlI	CTYRAG	1 cut(s)	1815
SmoI	CTYRAG	1 cut(s)	1815
SpeI	ACTAGT	1 cut(s)	164
SsiI	CCGC	5 cut(s)	236, 1918, 2077, 2083, 2515
SspMI	CTAG	3 cut(s)	165, 528, 2592
StyI	CCWWGG	4 cut(s)	19, 527, 996, 1696
TaaI	ACNGT	7 cut(s)	211, 369, 658, 922, 1409, 1882, 2401
TaiI	ACGT	5 cut(s)	539, 1062, 1588, 2038, 2431
TaqI	TCGA	1 cut(s)	1481
TatI	WGTACW	2 cut(s)	1069, 1304
TfiI	GAWTC	8 cut(s)	256, 383, 620, 845, 1277, 1379, 1391, 2578
TscAI	CASTG	7 cut(s)	216, 268, 345, 967, 1089, 1414, 1693
TseFI	GTSAC	4 cut(s)	637, 952, 1055, 2036
Tsp45I	GTSAC	4 cut(s)	637, 952, 1055, 2036
TspDTI	ATGAA	7 cut(s)	39, 240, 786, 1038, 1560, 1674, 1788
TspGWI	ACGGA	4 cut(s)	453, 492, 667, 2601
TspRI	CASTG	7 cut(s)	216, 268, 345, 967, 1089, 1414, 1693
Van91I	CCANNNNNTGG	2 cut(s)	20, 68
VneI	GTGCAC	1 cut(s)	2322
VpaK11BI	GGWCC	5 cut(s)	239, 566, 1557, 1780, 1896
XapI	RAATTY	2 cut(s)	664, 925
XceI	RCATGY	4 cut(s)	415, 455, 590, 2300
XcmI	CCANNNNNNNNNTGG	2 cut(s)	249, 1482
XmaJI	CCTAGG	1 cut(s)	527
XmiI	GTMKAC	1 cut(s)	819
XmnI	GAANNNNTTC	1 cut(s)	1204
XspI	CTAG	3 cut(s)	165, 528, 2592

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.