pycom13g01290

Protein BASIC PENTACYSTEINE2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010032
Physical Location & Seq
Forward (+)
845748 .. 846566
819 bp
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UTR
Exon/CDS
Intron
pycom13g01290.1

Sequence Viewer

Length: 819 bp
ATGCGCAATTGGGGCTACTATGAGCCATCTTTTAAGGGTCATCTTAGCTTGCAGCTCATGTCAAGCATGGCAGAGCGCGACACTAAACCATTTGTCCCTGGGCGTGACCCTACAGTCATGGTTAGCGCCAATGGAGCCTATCACCCTCGGGATTGTGTTGTATCAGATGCTCAGCTTCCAATGAGCTATATGAGGGAGAGTTGGGTAAACCAGAGAGATAAGTTTCTCAATATGATGCCTGCCAATCCTAACTATGCTGCTGTTCTTCCAGAAACTTCAGGAGCCCATTCCTTGCAGATCTTACAGCCGCCGGAACCATCAAGGGATGAAAGGTTGGGTAGGATTGAAGAGCCAGTTGTCCCTAAGGAAGTTGGCACATCCAAGAAAAGACAGGGTGGGGGTGCACCAAAAGCCCCAAAAGTGAAAAAGCCCAGGAAGGCAAAGGATAATACCAATCCTTCAGTTCCTCGTGTGAAGCCAGCAAAGAAGAGTTTGGATGTTGTGATTAACGGGATTAATATGGATATCTCTGGTATTCCAATTCCCATCTGCTCATGCACTGGAGTTCCACAACAGTGTTATAGGTGGGGGTGTGGTGGTTGGCAATCTGCTTGTTGCACCACAAATGTATCTATGTATCCTCTGCCAATGAGTACTAAGCGACGCGGGGCAAGAATAGCTGGAAGAAAAATGAGTCAGGGTGCTTTCAAGAAGGTGTTGGAGAAGCTTGCAGCTGAAGGTTATAATTTTACTAACCCAATTGATCTAAGGTATCATTGGGCAAAACATGGTACCAACAAGTTCGTCACTCTCAGGTAG

Protein Analysis

273

Amino Acids

30.26

Weight (kDa)

9.84

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAGA_bind PF06217 1 - 272 3.9e-98 GAGA binding protein-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14685 AT1G14685 AT1G14685 AT1G14685
fragaria_vesca FvH4_4g35810
malus_domestica MD13G1015100.v1.1 MD16G1012800.v1.1
prunus_persica Prupe.1G338500_v2.0.a1 Prupe.1G338500_v2.0.a1
pyrus_communis pycom13g01290 pycom16g01200
rosa_chinensis RchiOBHm_Chr4g0445341
rosa_laevigata RLG00000005747
rosa_multiflora Rmu_sc0000050.1_g000011
rosa_roxburghii Rroxscaffold_5G00385860
rosa_rugosa Rorug04G0360900
rosa_samantha Rh4AG421900 Rh4BG433900 Rh4CG448800 Rh4DG429900
rosa_wichuraiana Rw4G036190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AasI GACNNNNNNGTC 1 cut(s) 113
Acc16I TGCGCA 1 cut(s) 5
Acc65I GGTACC 1 cut(s) 791
AccB1I GGYRCC 1 cut(s) 791
AccII CGCG 2 cut(s) 78, 666
AciI CCGC 2 cut(s) 308, 666
AcuI CTGAAG 3 cut(s) 261, 444, 756
AfaI GTAC 2 cut(s) 655, 793
AgsI TTSAA 2 cut(s) 347, 709
AjnI CCWGG 2 cut(s) 97, 431
AleI CACNNNNGTG 1 cut(s) 574
AluBI AGCT 7 cut(s) 48, 55, 175, 186, 680, 727, 734
AluI AGCT 7 cut(s) 48, 55, 175, 186, 680, 727, 734
Alw21I GWGCWC 1 cut(s) 406
Alw44I GTGCAC 1 cut(s) 402
Ama87I CYCGRG 1 cut(s) 147
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 3 cut(s) 52, 257, 731
AseI ATTAAT 1 cut(s) 516
Asp718I GGTACC 1 cut(s) 791
AspLEI GCGC 3 cut(s) 6, 78, 128
AsuHPI GGTGA 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 147
AxyI CCTNAGG 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 406
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
BanI GGYRCC 1 cut(s) 791
BanII GRGCYC 1 cut(s) 286
BauI CACGAG 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 3 cut(s) 64, 244, 743
BccI CCATC 3 cut(s) 34, 325, 554
BciT130I CCWGG 2 cut(s) 99, 433
BciVI GTATCC 1 cut(s) 648
BfmI CTRYAG 1 cut(s) 111
BfoI RGCGCY 1 cut(s) 129
BfuI GTATCC 1 cut(s) 648
BglII AGATCT 1 cut(s) 297
BisI GCNGC 4 cut(s) 53, 258, 308, 732
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 4 cut(s) 54, 259, 309, 733
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 2 cut(s) 99, 433
BmeT110I CYCGRG 1 cut(s) 147
BmiI GGNNCC 4 cut(s) 136, 283, 315, 793
BmrFI CCNGG 2 cut(s) 99, 433
BmsI GCATC 2 cut(s) 157, 225
BpmI CTGGAG 1 cut(s) 582
Bpu1102I GCTNAGC 1 cut(s) 171
BsaJI CCNNGG 4 cut(s) 97, 98, 146, 431
Bse1I ACTGG 2 cut(s) 353, 565
Bse21I CCTNAGG 1 cut(s) 363
BseBI CCWGG 2 cut(s) 99, 433
BseDI CCNNGG 4 cut(s) 97, 98, 146, 431
BseGI GGATG 3 cut(s) 331, 377, 502
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 2 cut(s) 353, 565
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 3 cut(s) 64, 244, 743
Bsh1236I CGCG 2 cut(s) 78, 666
BshNI GGYRCC 1 cut(s) 791
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 2 cut(s) 80, 344
BsmFI GGGAC 2 cut(s) 80, 344
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 286, 406
Bsp143I GATC 2 cut(s) 297, 763
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 2 cut(s) 308, 666
BspCNI CTCAG 1 cut(s) 184
BspFNI CGCG 2 cut(s) 78, 666
BspLI GGNNCC 4 cut(s) 136, 283, 315, 793
BspQI GCTCTTC 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 791
BsrI ACTGG 2 cut(s) 353, 565
BssECI CCNNGG 4 cut(s) 97, 98, 146, 431
BssMI GATC 2 cut(s) 297, 763
BssSI CACGAG 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 2 cut(s) 99, 433
Bst4CI ACNGT 2 cut(s) 115, 576
Bst6I CTCTTC 2 cut(s) 342, 482
BstC8I GCNNGC 4 cut(s) 50, 240, 480, 729
BstDEI CTNAG 6 cut(s) 44, 171, 363, 657, 767, 812
BstF5I GGATG 3 cut(s) 331, 377, 502
BstFNI CGCG 2 cut(s) 78, 666
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 3 cut(s) 6, 78, 128
BstKTI GATC 2 cut(s) 300, 766
BstMBI GATC 2 cut(s) 297, 763
BstMWI GCNNNNNNNGC 4 cut(s) 12, 134, 410, 677
BstNI CCWGG 2 cut(s) 99, 433
BstSCI CCNGG 2 cut(s) 97, 431
BstSFI CTRYAG 1 cut(s) 111
BstSLI GKGCMC 1 cut(s) 406
BstUI CGCG 2 cut(s) 78, 666
BstV1I GCAGC 3 cut(s) 64, 244, 743
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 648
BtsCI GGATG 3 cut(s) 331, 377, 502
BtsIMutI CAGTG 2 cut(s) 558, 581
Cac8I GCNNGC 4 cut(s) 50, 240, 480, 729
CfoI GCGC 3 cut(s) 6, 78, 128
CseI GACGC 1 cut(s) 672
Csp6I GTAC 2 cut(s) 654, 792
CviAII CATG 5 cut(s) 58, 67, 118, 555, 788
CviQI GTAC 2 cut(s) 654, 792
DdeI CTNAG 6 cut(s) 44, 171, 363, 657, 767, 812
DpnI GATC 2 cut(s) 299, 765
DpnII GATC 2 cut(s) 297, 763
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
Eam1104I CTCTTC 2 cut(s) 342, 482
EarI CTCTTC 2 cut(s) 342, 482
Eco24I GRGCYC 1 cut(s) 286
Eco32I GATATC 1 cut(s) 526
Eco57I CTGAAG 3 cut(s) 261, 444, 756
Eco81I CCTNAGG 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 97, 431
EcoRV GATATC 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 286
FaeI CATG 5 cut(s) 61, 70, 121, 558, 791
FaqI GGGAC 2 cut(s) 80, 344
FatI CATG 5 cut(s) 57, 66, 117, 554, 787
FauI CCCGC 1 cut(s) 659
Fnu4HI GCNGC 4 cut(s) 53, 258, 308, 732
FokI GGATG 3 cut(s) 338, 364, 509
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 4 cut(s) 53, 258, 308, 732
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 77, 127
GluI GCNGC 4 cut(s) 53, 258, 308, 732
GsuI CTGGAG 1 cut(s) 582
HaeII RGCGCY 1 cut(s) 129
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 672
HhaI GCGC 3 cut(s) 6, 78, 128
Hin1II CATG 5 cut(s) 61, 70, 121, 558, 791
Hin6I GCGC 3 cut(s) 4, 76, 126
HinP1I GCGC 3 cut(s) 4, 76, 126
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 1 cut(s) 694
HpaII CCGG 1 cut(s) 311
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 2 cut(s) 208, 404
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 4 cut(s) 149, 269, 279, 709
Hpy8I GTNNAC 2 cut(s) 208, 404
Hpy99I CGWCG 1 cut(s) 666
HpyAV CCTTC 4 cut(s) 430, 468, 706, 731
HpyCH4III ACNGT 2 cut(s) 115, 576
HpyCH4V TGCA 6 cut(s) 52, 295, 404, 558, 618, 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 134, 410, 677
HpyF3I CTNAG 6 cut(s) 44, 171, 363, 657, 767, 812
Hsp92II CATG 5 cut(s) 61, 70, 121, 558, 791
HspAI GCGC 3 cut(s) 4, 76, 126
KpnI GGTACC 1 cut(s) 795
Kzo9I GATC 2 cut(s) 297, 763
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 2 cut(s) 134, 281
Lsp1109I GCAGC 3 cut(s) 64, 244, 743
LweI GCATC 2 cut(s) 157, 225
MaeIII GTNAC 2 cut(s) 104, 805
MalI GATC 2 cut(s) 299, 765
MboI GATC 2 cut(s) 297, 763
MboII GAAGA 4 cut(s) 257, 359, 499, 696
MfeI CAATTG 2 cut(s) 7, 759
MflI RGATCY 1 cut(s) 297
MhlI GDGCHC 2 cut(s) 286, 406
MluCI AATT 4 cut(s) 7, 540, 745, 759
MlyI GAGTC 1 cut(s) 703
MmeI TCCRAC 1 cut(s) 699
MnlI CCTC 4 cut(s) 156, 186, 477, 651
MseI TTAA 3 cut(s) 33, 507, 516
MslI CAYNNNNRTG 1 cut(s) 574
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 2 cut(s) 99, 433
MunI CAATTG 2 cut(s) 7, 759
MvaI CCWGG 2 cut(s) 99, 433
MvnI CGCG 2 cut(s) 78, 666
MwoI GCNNNNNNNGC 4 cut(s) 12, 134, 410, 677
NdeII GATC 2 cut(s) 297, 763
NlaIII CATG 5 cut(s) 61, 70, 121, 558, 791
NlaIV GGNNCC 4 cut(s) 136, 283, 315, 793
NmuCI GTSAC 2 cut(s) 104, 805
NsbI TGCGCA 1 cut(s) 5
OliI CACNNNNGTG 1 cut(s) 574
PasI CCCWGGG 1 cut(s) 98
PciSI GCTCTTC 1 cut(s) 342
PkrI GCNGC 4 cut(s) 54, 259, 309, 733
PleI GAGTC 1 cut(s) 702
PpsI GAGTC 1 cut(s) 702
PshBI ATTAAT 1 cut(s) 516
PsiI TTATAA 1 cut(s) 744
Psp6I CCWGG 2 cut(s) 97, 431
PspGI CCWGG 2 cut(s) 97, 431
PspN4I GGNNCC 4 cut(s) 136, 283, 315, 793
PsuI RGATCY 1 cut(s) 297
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 2 cut(s) 655, 793
RsaNI GTAC 2 cut(s) 654, 792
RseI CAYNNNNRTG 1 cut(s) 574
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 3 cut(s) 33, 507, 516
SatI GCNGC 4 cut(s) 53, 258, 308, 732
Sau3AI GATC 2 cut(s) 297, 763
ScaI AGTACT 1 cut(s) 655
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 2 cut(s) 99, 433
SduI GDGCHC 2 cut(s) 286, 406
SfaNI GCATC 2 cut(s) 157, 225
SfcI CTRYAG 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 574
Sse9I AATT 4 cut(s) 7, 540, 745, 759
SsiI CCGC 2 cut(s) 308, 666
StyD4I CCNGG 2 cut(s) 97, 431
TaaI ACNGT 2 cut(s) 115, 576
TasI AATT 4 cut(s) 7, 540, 745, 759
TatI WGTACW 1 cut(s) 653
TauI GCSGC 1 cut(s) 310
Tru1I TTAA 3 cut(s) 33, 507, 516
Tru9I TTAA 3 cut(s) 33, 507, 516
TscAI CASTG 2 cut(s) 565, 581
TseFI GTSAC 2 cut(s) 104, 805
TseI GCWGC 3 cut(s) 52, 257, 731
Tsp45I GTSAC 2 cut(s) 104, 805
TspDTI ATGAA 1 cut(s) 342
TspRI CASTG 2 cut(s) 565, 581
VneI GTGCAC 1 cut(s) 402
VspI ATTAAT 1 cut(s) 516
ZrmI AGTACT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.