pycom16g01200

Protein BASIC PENTACYSTEINE2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010032
Physical Location & Seq
Forward (+)
755151 .. 757060
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g01200.1

Sequence Viewer

Length: 1008 bp
ATGGAGGGCTTTCTTTTTCTCTATCTCAGAAGTTTCTCTCTCTCCTTCTCTCTCAGGTTTCTCTCTCCTCTCTCCTCTCTCTTCCCTCTCTCTCTCCAGAAGGGCAAAACCCTAAAGAGCCGTTTACACCCACCCAAGTTTGTTTGTCTTGCTGAATCTGTAGCTCTCATGGATGATGATGCATTGAACATGCGCAATTGGGGTTACTATGAGCCATCTTTTAAAGGTCATCTTAGCCTGCAGCTCATGTCAAGCATGGCAGAGCGCGACATTAAACCTTTTGCCCCTGGGCGCGATCCTGCAGTCATGGTTAGTGCCAATGGAGCCTATCACCCTCGGGATTGTGTTGTATCAGACACTCAGCTTCCAATGAACTATATGAGGGAGAGTTGGGTAAACCAGCGAGATAAATTTCTCAATATGATGCCTGCCAATCCTAACTATGCTGCTGTTCTTCCGGAAACTTCAGGAGCTCATTCCTTGCAGATCTTACAGCCACCGGAAGCATCAAGGGATGAGCGGGTGGGTAGGATGGAAGAGCCAGTTGTCCCTAAGGAAGGTGGCACCTCAAAGAAAAGACAGGGTGGGGGTGCACCAAAAGCCCCAAAAGTGAAAAAGCCCAGGAAGCCTAAGGATAATGCCAATCCTTCAGTTCCTCGTGTGAAGCCAGCAAAGAAGAGTTTGGACGTTGTGATAAATGGGATTAATATGGATATCTCTGGTATTCCAATTCCTGTCTGCTCGTGCACTGGAGCTCCACAACAGTGTTATAGGTGGGGGTGTGGTGGTTGGCAATCTGCTTGTTGCACCACAAATGTATCTATGTATCCTCTGCCAATGAGTACCAAGCGACGCGGGGCAAGAATAGCTGGAAGAAAAATGAGTCAGGGTGCTTTCAAGAAGGTGTTGGAGAAGCTTGCAGCTGAAGGTTATAATTTTGCTAACCCAATCGATCTAAGGTCTCACTGGGCAAAACATGGTACCAACAAGTTCGTCACTCTCAGGTAG

Protein Analysis

336

Amino Acids

37.16

Weight (kDa)

9.82

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAGA_bind PF06217 57 - 335 3.2e-99 GAGA binding protein-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14685 AT1G14685 AT1G14685 AT1G14685
fragaria_vesca FvH4_4g35810
malus_domestica MD13G1015100.v1.1 MD16G1012800.v1.1
prunus_persica Prupe.1G338500_v2.0.a1 Prupe.1G338500_v2.0.a1
pyrus_communis pycom13g01290 pycom16g01200
rosa_chinensis RchiOBHm_Chr4g0445341
rosa_laevigata RLG00000005747
rosa_multiflora Rmu_sc0000050.1_g000011
rosa_roxburghii Rroxscaffold_5G00385860
rosa_rugosa Rorug04G0360900
rosa_samantha Rh4AG421900 Rh4BG433900 Rh4CG448800 Rh4DG429900
rosa_wichuraiana Rw4G036190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
Acc16I TGCGCA 1 cut(s) 194
Acc65I GGTACC 1 cut(s) 980
AccB1I GGYRCC 2 cut(s) 563, 980
AccBSI CCGCTC 1 cut(s) 520
AccII CGCG 3 cut(s) 267, 294, 855
AccIII TCCGGA 1 cut(s) 457
AciI CCGC 2 cut(s) 520, 855
AclWI GGATC 1 cut(s) 290
AcsI RAATTY 1 cut(s) 410
AcuI CTGAAG 3 cut(s) 450, 633, 945
AfaI GTAC 2 cut(s) 844, 982
AfiI CCNNNNNNNGG 1 cut(s) 557
AgsI TTSAA 2 cut(s) 187, 898
AjnI CCWGG 2 cut(s) 286, 620
AleI CACNNNNGTG 1 cut(s) 763
AluBI AGCT 8 cut(s) 164, 244, 364, 473, 755, 869, 916, 923
AluI AGCT 8 cut(s) 164, 244, 364, 473, 755, 869, 916, 923
Alw21I GWGCWC 4 cut(s) 475, 595, 749, 757
Alw26I GTCTC 1 cut(s) 966
Alw44I GTGCAC 2 cut(s) 591, 745
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 336
Aor13HI TCCGGA 1 cut(s) 457
ApaLI GTGCAC 2 cut(s) 591, 745
ApeKI GCWGC 3 cut(s) 241, 446, 920
ApoI RAATTY 1 cut(s) 410
AseI ATTAAT 1 cut(s) 705
Asp718I GGTACC 1 cut(s) 980
AspLEI GCGC 3 cut(s) 195, 267, 294
AsuHPI GGTGA 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 336
AxyI CCTNAGG 2 cut(s) 552, 630
BaeGI GKGCMC 2 cut(s) 595, 749
BaeI ACNNNNGTAYC 2 cut(s) 801, 834
BanI GGYRCC 2 cut(s) 563, 980
BanII GRGCYC 2 cut(s) 475, 757
BauI CACGAG 2 cut(s) 657, 742
Bbv12I GWGCWC 4 cut(s) 475, 595, 749, 757
BbvI GCAGC 3 cut(s) 253, 433, 932
BccI CCATC 2 cut(s) 223, 526
BceAI ACGGC 1 cut(s) 105
BciT130I CCWGG 2 cut(s) 288, 622
BciVI GTATCC 1 cut(s) 837
BcoDI GTCTC 1 cut(s) 966
BfmI CTRYAG 3 cut(s) 159, 239, 300
BfuI GTATCC 1 cut(s) 837
BglII AGATCT 1 cut(s) 486
BisI GCNGC 3 cut(s) 242, 447, 921
BlsI GCNGC 3 cut(s) 243, 448, 922
Bme1390I CCNGG 2 cut(s) 288, 622
BmeT110I CYCGRG 1 cut(s) 336
BmiI GGNNCC 3 cut(s) 325, 565, 982
BmrFI CCNGG 2 cut(s) 288, 622
BmrI ACTGGG 1 cut(s) 976
BmsI GCATC 3 cut(s) 169, 414, 515
BmuI ACTGGG 1 cut(s) 976
BpmI CTGGAG 2 cut(s) 80, 771
Bsa29I ATCGAT 1 cut(s) 951
BsaI GGTCTC 1 cut(s) 966
BsaJI CCNNGG 4 cut(s) 286, 287, 335, 620
BsaWI WCCGGW 2 cut(s) 457, 499
BsaXI ACNNNNNCTCC 2 cut(s) 739, 769
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse1I ACTGG 3 cut(s) 542, 754, 971
Bse21I CCTNAGG 2 cut(s) 552, 630
BseAI TCCGGA 1 cut(s) 457
BseBI CCWGG 2 cut(s) 288, 622
BseCI ATCGAT 1 cut(s) 951
BseDI CCNNGG 4 cut(s) 286, 287, 335, 620
BseGI GGATG 3 cut(s) 178, 520, 537
BseLI CCNNNNNNNGG 1 cut(s) 557
BseMII CTCAG 3 cut(s) 40, 67, 374
BseNI ACTGG 3 cut(s) 542, 754, 971
BseRI GAGGAG 2 cut(s) 57, 64
BseSI GKGCMC 2 cut(s) 595, 749
BseXI GCAGC 3 cut(s) 253, 433, 932
Bsh1236I CGCG 3 cut(s) 267, 294, 855
BshNI GGYRCC 2 cut(s) 563, 980
BshVI ATCGAT 1 cut(s) 951
BsiHKAI GWGCWC 4 cut(s) 475, 595, 749, 757
BsiHKCI CYCGRG 1 cut(s) 336
BsiSI CCGG 2 cut(s) 458, 500
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 1 cut(s) 557
BsmAI GTCTC 1 cut(s) 966
BsmFI GGGAC 1 cut(s) 533
Bso31I GGTCTC 1 cut(s) 966
BsoBI CYCGRG 1 cut(s) 336
Bsp1286I GDGCHC 4 cut(s) 475, 595, 749, 757
Bsp13I TCCGGA 1 cut(s) 457
Bsp143I GATC 3 cut(s) 295, 486, 952
BspACI CCGC 2 cut(s) 520, 855
BspCNI CTCAG 3 cut(s) 39, 66, 373
BspDI ATCGAT 1 cut(s) 951
BspEI TCCGGA 1 cut(s) 457
BspFNI CGCG 3 cut(s) 267, 294, 855
BspLI GGNNCC 3 cut(s) 325, 565, 982
BspMAI CTGCAG 2 cut(s) 243, 304
BspPI GGATC 1 cut(s) 290
BspQI GCTCTTC 1 cut(s) 531
BspT107I GGYRCC 2 cut(s) 563, 980
BspTNI GGTCTC 1 cut(s) 966
BsrBI CCGCTC 1 cut(s) 520
BsrI ACTGG 3 cut(s) 542, 754, 971
BssECI CCNNGG 4 cut(s) 286, 287, 335, 620
BssMI GATC 3 cut(s) 295, 486, 952
BssSI CACGAG 2 cut(s) 657, 742
Bst2BI CACGAG 2 cut(s) 657, 742
Bst2UI CCWGG 2 cut(s) 288, 622
Bst4CI ACNGT 1 cut(s) 765
Bst6I CTCTTC 3 cut(s) 86, 531, 671
BstC8I GCNNGC 4 cut(s) 239, 429, 669, 918
BstDEI CTNAG 8 cut(s) 26, 53, 233, 360, 552, 630, 956, 1001
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 3 cut(s) 178, 520, 537
BstFNI CGCG 3 cut(s) 267, 294, 855
BstHHI GCGC 3 cut(s) 195, 267, 294
BstKTI GATC 3 cut(s) 298, 489, 955
BstMAI GTCTC 1 cut(s) 966
BstMBI GATC 3 cut(s) 295, 486, 952
BstMWI GCNNNNNNNGC 4 cut(s) 323, 599, 625, 866
BstNI CCWGG 2 cut(s) 288, 622
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 286, 620
BstSFI CTRYAG 3 cut(s) 159, 239, 300
BstSLI GKGCMC 2 cut(s) 595, 749
BstUI CGCG 3 cut(s) 267, 294, 855
BstV1I GCAGC 3 cut(s) 253, 433, 932
BstX2I RGATCY 1 cut(s) 486
BstYI RGATCY 1 cut(s) 486
Bsu15I ATCGAT 1 cut(s) 951
Bsu36I CCTNAGG 2 cut(s) 552, 630
BsuI GTATCC 1 cut(s) 837
BsuTUI ATCGAT 1 cut(s) 951
BtsCI GGATG 3 cut(s) 178, 520, 537
BtsIMutI CAGTG 3 cut(s) 747, 770, 964
Cac8I GCNNGC 4 cut(s) 239, 429, 669, 918
CfoI GCGC 3 cut(s) 195, 267, 294
ClaI ATCGAT 1 cut(s) 951
CseI GACGC 1 cut(s) 861
Csp6I GTAC 2 cut(s) 843, 981
CviAII CATG 6 cut(s) 169, 190, 247, 256, 307, 977
CviQI GTAC 2 cut(s) 843, 981
DdeI CTNAG 8 cut(s) 26, 53, 233, 360, 552, 630, 956, 1001
DpnI GATC 3 cut(s) 297, 488, 954
DpnII GATC 3 cut(s) 295, 486, 952
DraI TTTAAA 1 cut(s) 223
Eam1104I CTCTTC 3 cut(s) 86, 531, 671
EarI CTCTTC 3 cut(s) 86, 531, 671
Ecl136II GAGCTC 2 cut(s) 473, 755
Eco24I GRGCYC 2 cut(s) 475, 757
Eco31I GGTCTC 1 cut(s) 966
Eco32I GATATC 1 cut(s) 715
Eco53kI GAGCTC 2 cut(s) 473, 755
Eco57I CTGAAG 3 cut(s) 450, 633, 945
Eco81I CCTNAGG 2 cut(s) 552, 630
Eco88I CYCGRG 1 cut(s) 336
EcoICRI GAGCTC 2 cut(s) 473, 755
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoRII CCWGG 2 cut(s) 286, 620
EcoRV GATATC 1 cut(s) 715
EcoT22I ATGCAT 1 cut(s) 184
EcoT38I GRGCYC 2 cut(s) 475, 757
FaeI CATG 6 cut(s) 172, 193, 250, 259, 310, 980
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 1 cut(s) 533
FatI CATG 6 cut(s) 168, 189, 246, 255, 306, 976
FauI CCCGC 2 cut(s) 513, 848
Fnu4HI GCNGC 3 cut(s) 242, 447, 921
FokI GGATG 3 cut(s) 185, 527, 544
FriOI GRGCYC 2 cut(s) 475, 757
Fsp4HI GCNGC 3 cut(s) 242, 447, 921
FspI TGCGCA 1 cut(s) 194
GlaI GCGC 3 cut(s) 194, 266, 293
GluI GCNGC 3 cut(s) 242, 447, 921
GsuI CTGGAG 2 cut(s) 80, 771
HapII CCGG 2 cut(s) 458, 500
HgaI GACGC 1 cut(s) 861
HhaI GCGC 3 cut(s) 195, 267, 294
Hin1II CATG 6 cut(s) 172, 193, 250, 259, 310, 980
Hin6I GCGC 3 cut(s) 193, 265, 292
HinP1I GCGC 3 cut(s) 193, 265, 292
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 2 cut(s) 155, 883
HpaII CCGG 2 cut(s) 458, 500
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 4 cut(s) 125, 397, 593, 747
Hpy188I TCNGA 2 cut(s) 29, 355
Hpy188III TCNNGA 5 cut(s) 97, 338, 458, 468, 898
Hpy8I GTNNAC 4 cut(s) 125, 397, 593, 747
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 6 cut(s) 55, 94, 551, 657, 895, 920
HpyCH4III ACNGT 1 cut(s) 765
HpyCH4IV ACGT 1 cut(s) 687
HpyCH4V TGCA 8 cut(s) 182, 241, 302, 484, 593, 747, 807, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 599, 625, 866
HpyF3I CTNAG 8 cut(s) 26, 53, 233, 360, 552, 630, 956, 1001
HpySE526I ACGT 1 cut(s) 687
Hsp92II CATG 6 cut(s) 172, 193, 250, 259, 310, 980
HspAI GCGC 3 cut(s) 193, 265, 292
Kpn2I TCCGGA 1 cut(s) 457
KpnI GGTACC 1 cut(s) 984
Kzo9I GATC 3 cut(s) 295, 486, 952
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 4 cut(s) 323, 470, 752, 760
Lsp1109I GCAGC 3 cut(s) 253, 433, 932
LweI GCATC 3 cut(s) 169, 414, 515
MaeII ACGT 1 cut(s) 687
MaeIII GTNAC 2 cut(s) 203, 994
MalI GATC 3 cut(s) 297, 488, 954
MbiI CCGCTC 1 cut(s) 520
MboI GATC 3 cut(s) 295, 486, 952
MboII GAAGA 5 cut(s) 73, 446, 548, 688, 885
MfeI CAATTG 1 cut(s) 196
MflI RGATCY 1 cut(s) 486
MhlI GDGCHC 4 cut(s) 475, 595, 749, 757
MluCI AATT 4 cut(s) 196, 410, 729, 934
MlyI GAGTC 1 cut(s) 892
MmeI TCCRAC 1 cut(s) 888
MnlI CCTC 8 cut(s) 78, 85, 96, 345, 375, 577, 666, 840
Mph1103I ATGCAT 1 cut(s) 184
MroI TCCGGA 1 cut(s) 457
MseI TTAA 3 cut(s) 222, 273, 705
MslI CAYNNNNRTG 1 cut(s) 763
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 2 cut(s) 458, 500
MspR9I CCNGG 2 cut(s) 288, 622
MunI CAATTG 1 cut(s) 196
MvaI CCWGG 2 cut(s) 288, 622
MvnI CGCG 3 cut(s) 267, 294, 855
MwoI GCNNNNNNNGC 4 cut(s) 323, 599, 625, 866
NdeII GATC 3 cut(s) 295, 486, 952
NlaIII CATG 6 cut(s) 172, 193, 250, 259, 310, 980
NlaIV GGNNCC 3 cut(s) 325, 565, 982
NmuCI GTSAC 1 cut(s) 994
NsbI TGCGCA 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 1 cut(s) 193
OliI CACNNNNGTG 1 cut(s) 763
PasI CCCWGGG 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 531
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 3 cut(s) 243, 448, 922
PleI GAGTC 1 cut(s) 891
PpsI GAGTC 1 cut(s) 891
PshBI ATTAAT 1 cut(s) 705
PsiI TTATAA 1 cut(s) 933
Psp124BI GAGCTC 2 cut(s) 475, 757
Psp6I CCWGG 2 cut(s) 286, 620
PspGI CCWGG 2 cut(s) 286, 620
PspN4I GGNNCC 3 cut(s) 325, 565, 982
PstI CTGCAG 2 cut(s) 243, 304
PsuI RGATCY 1 cut(s) 486
PvuII CAGCTG 1 cut(s) 923
RsaI GTAC 2 cut(s) 844, 982
RsaNI GTAC 2 cut(s) 843, 981
RseI CAYNNNNRTG 1 cut(s) 763
SacI GAGCTC 2 cut(s) 475, 757
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 3 cut(s) 222, 273, 705
SatI GCNGC 3 cut(s) 242, 447, 921
Sau3AI GATC 3 cut(s) 295, 486, 952
SchI GAGTC 1 cut(s) 892
ScrFI CCNGG 2 cut(s) 288, 622
SduI GDGCHC 4 cut(s) 475, 595, 749, 757
SfaNI GCATC 3 cut(s) 169, 414, 515
SfcI CTRYAG 3 cut(s) 159, 239, 300
SmiMI CAYNNNNRTG 1 cut(s) 763
Sse9I AATT 4 cut(s) 196, 410, 729, 934
SsiI CCGC 2 cut(s) 520, 855
SstI GAGCTC 2 cut(s) 475, 757
StyD4I CCNGG 2 cut(s) 286, 620
TaaI ACNGT 1 cut(s) 765
TaiI ACGT 1 cut(s) 690
TaqI TCGA 1 cut(s) 951
TasI AATT 4 cut(s) 196, 410, 729, 934
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 3 cut(s) 222, 273, 705
Tru9I TTAA 3 cut(s) 222, 273, 705
TscAI CASTG 3 cut(s) 754, 770, 971
TseFI GTSAC 1 cut(s) 994
TseI GCWGC 3 cut(s) 241, 446, 920
Tsp45I GTSAC 1 cut(s) 994
TspDTI ATGAA 1 cut(s) 386
TspRI CASTG 3 cut(s) 754, 770, 971
VneI GTGCAC 2 cut(s) 591, 745
VspI ATTAAT 1 cut(s) 705
XagI CCTNNNNNAGG 1 cut(s) 555
XapI RAATTY 1 cut(s) 410
XceI RCATGY 1 cut(s) 193
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.