pycom13g01900

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009650
Physical Location & Seq
Reverse (-)
1280273 .. 1281490
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGGTGTGTGTACGTATATGCCCCAACAGTGTGCAAAAAAAGATGTCGGGTGGATGGACAACTGGCCTGCTTGACTGCTTCAGTGACTGCAGTGTCTGCTGCTTGGGATTTTGGTGTCCTTGCGTTGTTGCTGGGCGTGTTGCAGAGATTGTCTCCAAAGGACATACCGGTTGTTTTCAGCAGGGATGTTTGTATGCACTAATCAATTCCTATACTCAGTTAGCATTCTGCATTCCTTGCGGTTTCTGCATTACTTGCGGCTTTCGAACCAAACTGAGGGAGCACTACAAGTTGGAGGAAAAACCTTGCAACGATTGCTGTGTTCATTTCTTCTGCCACTCCTGTGCCTTGTGCCAAGAATATCGTGAGCTCGAGAACCGTGGTTTCACTGTTGCCATTGGCTGGGAACAAAATGCGTCACAGAATCCTGGAGTGGTGACACCACCATCTGTGCAAGGTGACATGAATCGTCAGACTGATCACAAATAG

Protein Analysis

163

Amino Acids

18.02

Weight (kDa)

7.38

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 19 - 123 7.3e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 402
AciI CCGC 2 cut(s) 240, 258
AcuI CTGAAG 1 cut(s) 64
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 2 cut(s) 276, 402
AgeI ACCGGT 1 cut(s) 167
AjnI CCWGG 1 cut(s) 427
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 1 cut(s) 370
AluI AGCT 1 cut(s) 370
Alw21I GWGCWC 2 cut(s) 285, 372
Alw26I GTCTC 1 cut(s) 157
AlwNI CAGNNNCTG 2 cut(s) 87, 96
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 1 cut(s) 99
AsiGI ACCGGT 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 448, 470
AsuII TTCGAA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 371
BanII GRGCYC 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 285, 372
BbvI GCAGC 1 cut(s) 86
BccI CCATC 2 cut(s) 48, 454
BciT130I CCWGG 1 cut(s) 429
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 1 cut(s) 157
BfmI CTRYAG 1 cut(s) 88
BisI GCNGC 2 cut(s) 100, 259
BlsI GCNGC 2 cut(s) 101, 260
Bme1390I CCNGG 1 cut(s) 429
BmeT110I CYCGRG 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 429
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 265
BsaAI YACGTR 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 379
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 402
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 67
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 2 cut(s) 59, 191
BseLI CCNNNNNNNGG 2 cut(s) 276, 402
BseMII CTCAG 2 cut(s) 230, 266
BseNI ACTGG 1 cut(s) 67
BseXI GCAGC 1 cut(s) 86
BseYI CCCAGC 2 cut(s) 131, 402
BshFI GGCC 1 cut(s) 66
BshTI ACCGGT 1 cut(s) 167
BsiHKAI GWGCWC 2 cut(s) 285, 372
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 168
BslI CCNNNNNNNGG 2 cut(s) 276, 402
BsmAI GTCTC 1 cut(s) 157
BsmI GAATGC 2 cut(s) 224, 231
BsnI GGCC 1 cut(s) 66
BsoBI CYCGRG 1 cut(s) 371
Bsp119I TTCGAA 1 cut(s) 265
Bsp1286I GDGCHC 2 cut(s) 285, 372
Bsp143I GATC 1 cut(s) 478
BspACI CCGC 2 cut(s) 240, 258
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 2 cut(s) 229, 267
BspMAI CTGCAG 1 cut(s) 92
BspT104I TTCGAA 1 cut(s) 265
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 1 cut(s) 67
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 1 cut(s) 478
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 3 cut(s) 29, 380, 391
BstAPI GCANNNNNTGC 4 cut(s) 96, 237, 255, 315
BstBAI YACGTR 1 cut(s) 14
BstBI TTCGAA 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 2 cut(s) 216, 275
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 2 cut(s) 59, 191
BstKTI GATC 1 cut(s) 481
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 1 cut(s) 478
BstMWI GCNNNNNNNGC 5 cut(s) 96, 237, 246, 255, 315
BstNI CCWGG 1 cut(s) 429
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 1 cut(s) 88
BstSNI TACGTA 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 86
BsuRI GGCC 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 379
BtsCI GGATG 2 cut(s) 59, 191
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 4 cut(s) 34, 88, 97, 387
Cac8I GCNNGC 1 cut(s) 68
CaiI CAGNNNCTG 2 cut(s) 87, 96
Cfr10I RCCGGY 1 cut(s) 167
CseI GACGC 1 cut(s) 405
Csp6I GTAC 1 cut(s) 11
CspAI ACCGGT 1 cut(s) 167
CviAII CATG 1 cut(s) 463
CviJI RGCY 4 cut(s) 66, 261, 370, 402
CviKI_1 RGCY 4 cut(s) 66, 261, 370, 402
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 2 cut(s) 216, 275
DpnI GATC 1 cut(s) 480
DpnII GATC 1 cut(s) 478
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 370
Eco105I TACGTA 1 cut(s) 14
Eco24I GRGCYC 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 372
FaeI CATG 1 cut(s) 466
FaiI YATR 6 cut(s) 17, 19, 165, 195, 213, 464
FatI CATG 1 cut(s) 462
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 2 cut(s) 100, 259
FokI GGATG 2 cut(s) 66, 198
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 2 cut(s) 100, 259
GluI GCNGC 2 cut(s) 100, 259
GsaI CCCAGC 2 cut(s) 135, 406
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 1 cut(s) 66
HapII CCGG 1 cut(s) 168
HgaI GACGC 1 cut(s) 405
Hin1II CATG 1 cut(s) 466
HinfI GANTC 2 cut(s) 424, 466
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 448, 470
Hpy166II GTNNAC 1 cut(s) 11
Hpy188I TCNGA 1 cut(s) 474
Hpy188III TCNNGA 2 cut(s) 365, 373
Hpy8I GTNNAC 1 cut(s) 11
HpyCH4III ACNGT 3 cut(s) 29, 380, 391
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 8 cut(s) 34, 90, 143, 197, 231, 249, 309, 454
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 237, 246, 255, 315
HpyF3I CTNAG 2 cut(s) 216, 275
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 1 cut(s) 466
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 1 cut(s) 478
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 9 cut(s) 48, 80, 117, 167, 181, 355, 388, 414, 441
Lsp1109I GCAGC 1 cut(s) 86
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 4 cut(s) 83, 417, 436, 458
MalI GATC 1 cut(s) 480
MboI GATC 1 cut(s) 478
MboII GAAGA 1 cut(s) 322
MhlI GDGCHC 2 cut(s) 285, 372
MluCI AATT 1 cut(s) 205
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 2 cut(s) 270, 289
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 429
Mva1269I GAATGC 2 cut(s) 224, 231
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 5 cut(s) 96, 237, 246, 255, 315
NdeII GATC 1 cut(s) 478
NlaIII CATG 1 cut(s) 466
NmuCI GTSAC 4 cut(s) 83, 417, 436, 458
NspV TTCGAA 1 cut(s) 265
OliI CACNNNNGTG 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 371
PctI GAATGC 2 cut(s) 224, 231
PfeI GAWTC 2 cut(s) 424, 466
PflMI CCANNNNNTGG 1 cut(s) 402
PfoI TCCNGGA 1 cut(s) 427
PinAI ACCGGT 1 cut(s) 167
PkrI GCNGC 2 cut(s) 101, 260
Ppu21I YACGTR 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 427
PspFI CCCAGC 2 cut(s) 131, 402
PspGI CCWGG 1 cut(s) 427
PstI CTGCAG 1 cut(s) 92
PstNI CAGNNNCTG 2 cut(s) 87, 96
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 1 cut(s) 342
SacI GAGCTC 1 cut(s) 372
SatI GCNGC 2 cut(s) 100, 259
Sau3AI GATC 1 cut(s) 478
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 2 cut(s) 285, 372
SetI ASST 4 cut(s) 16, 307, 372, 460
SfcI CTRYAG 1 cut(s) 88
Sfr274I CTCGAG 1 cut(s) 371
SfuI TTCGAA 1 cut(s) 265
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SnaBI TACGTA 1 cut(s) 14
Sse9I AATT 1 cut(s) 205
SsiI CCGC 2 cut(s) 240, 258
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 29, 380, 391
TaiI ACGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 265, 372
TasI AATT 1 cut(s) 205
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 2 cut(s) 424, 466
TscAI CASTG 4 cut(s) 34, 88, 97, 394
TseFI GTSAC 4 cut(s) 83, 417, 436, 458
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 4 cut(s) 83, 417, 436, 458
TspDTI ATGAA 2 cut(s) 314, 479
TspRI CASTG 4 cut(s) 34, 88, 97, 394
Van91I CCANNNNNTGG 1 cut(s) 402
XhoI CTCGAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.