pycom13g02690

Repressor of RNA polymerase III transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010817
Physical Location & Seq
Forward (+)
1855084 .. 1857988
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 927 bp
ATGCATCAACACGCTAAAACTGCGGGGGGCTTGACTTTGAAGAGTAATCCACGAAGCACCAACCGCAAAGCAATTCAAAATTTGGAATATTCGAATTTTGGGGGCTGTCCGAAACGTTCTGGATCCCGTTCTTACCGAACCCTAACGCCCAAATCGGAACTCTCGTCGTCTTCGCCTTTTCCAATTCGTTATAATTTCCTCTCAGGATCTCCTTTTTCTGTAATAATTGAGTGCTTTCGACATTCGCTGAGCATGAAGTTCTTGGAATACACCCCGCTGGAGCGATTAAATGATTTCTTTAGTCATTTGAATCTCGGAGAGCGAACCATTAAGGGTTGTCTTGAAGCTTACTCTTGCAAACACACCGGATCCGATAAGAAATTGTCTCTCAGTTTGGAGAATGAGATTCTTGATTATCTTGGGAAATCGTCTGACAGTGATTCTTCCTCACCTGCTGAATTCCTGCTGACTAGATCCAGCCGGAAGACATTGATTTACCTGGTTTTGACACTCTATCACTTGTATCCGGACTATGACTTCAGTGCAGTAAATGCTCATCAGTTCTTCACAGAGGAAAGCTGGGACAGTTTTAAGCAGATTTTTGATACCTACATGTTTGAAGCATCAAAGGAATGGACAGAGACAAATGAGGGCAGTTCCTTGCTGGAAGCCCTTTACAAGGCTTTGGATGAGGTTGTTAGACTAGCGGAATGTGAAGTTTACAGCTACATTCCAGACTCGGATGCTGATCCGTTTCTTGAGAGAGGATCTATATGGTCGTTCAATTTCTTTTTCTACAATAGGAAACTTAAGCGTGTCGTGAGCTTCCGTGTCTGCTGTTTAAGTAACTTGGTGGCTGATGGATATCGGATGGACGAGTTGCGGTATGATGAAGATGGAGAAATTTTCGACAACATGGACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf1

Protein Analysis

309

Amino Acids

35.5

Weight (kDa)

5.48

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf1 PF09174 110 - 276 4.7e-52 Maf1 regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 192
AarI CACCTGC 1 cut(s) 460
Acc36I ACCTGC 1 cut(s) 460
AccIII TCCGGA 1 cut(s) 526
AciI CCGC 5 cut(s) 23, 64, 275, 707, 883
AclI AACGTT 1 cut(s) 115
AclWI GGATC 8 cut(s) 117, 130, 214, 363, 376, 468, 743, 775
AcsI RAATTY 4 cut(s) 79, 94, 458, 903
AcuI CTGAAG 1 cut(s) 523
AfiI CCNNNNNNNGG 1 cut(s) 679
AflII CTTAAG 1 cut(s) 809
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 6 cut(s) 40, 77, 310, 344, 620, 784
AjnI CCWGG 1 cut(s) 498
AluBI AGCT 4 cut(s) 347, 579, 726, 825
AluI AGCT 4 cut(s) 347, 579, 726, 825
Alw26I GTCTC 2 cut(s) 390, 635
AlwI GGATC 8 cut(s) 117, 130, 214, 363, 376, 468, 743, 775
Aor13HI TCCGGA 1 cut(s) 526
ApoI RAATTY 4 cut(s) 79, 94, 458, 903
AsuHPI GGTGA 1 cut(s) 441
AsuII TTCGAA 1 cut(s) 92
BamHI GGATCC 2 cut(s) 122, 368
BbsI GAAGAC 2 cut(s) 162, 491
BccI CCATC 3 cut(s) 854, 865, 890
BciT130I CCWGG 1 cut(s) 500
BciVI GTATCC 1 cut(s) 534
BcoDI GTCTC 2 cut(s) 390, 635
BfaI CTAG 2 cut(s) 471, 704
BfrI CTTAAG 1 cut(s) 809
BfuAI ACCTGC 1 cut(s) 460
BfuI GTATCC 1 cut(s) 534
BlpI GCTNAGC 1 cut(s) 248
Bme1390I CCNGG 1 cut(s) 500
BmiI GGNNCC 2 cut(s) 124, 370
BmrFI CCNGG 1 cut(s) 500
BmsI GCATC 3 cut(s) 13, 632, 733
BpiI GAAGAC 2 cut(s) 162, 491
BpmI CTGGAG 1 cut(s) 299
Bpu1102I GCTNAGC 1 cut(s) 248
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 779
BsaBI GATNNNNATC 2 cut(s) 747, 864
BsaWI WCCGGW 2 cut(s) 365, 526
Bsc4I CCNNNNNNNGG 1 cut(s) 679
Bse8I GATNNNNATC 2 cut(s) 747, 864
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 1 cut(s) 500
BseGI GGATG 3 cut(s) 694, 748, 876
BseJI GATNNNNATC 2 cut(s) 747, 864
BseLI CCNNNNNNNGG 1 cut(s) 679
BseMII CTCAG 3 cut(s) 216, 239, 403
BseYI CCCAGC 1 cut(s) 579
BsgI GTGCAG 1 cut(s) 564
BsiSI CCGG 3 cut(s) 366, 481, 527
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 679
BsmAI GTCTC 2 cut(s) 390, 635
BsmFI GGGAC 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 92
Bsp13I TCCGGA 1 cut(s) 526
Bsp143I GATC 6 cut(s) 122, 206, 368, 473, 748, 767
Bsp1720I GCTNAGC 1 cut(s) 248
BspACI CCGC 5 cut(s) 23, 64, 275, 707, 883
BspCNI CTCAG 3 cut(s) 215, 240, 402
BspEI TCCGGA 1 cut(s) 526
BspLI GGNNCC 2 cut(s) 124, 370
BspMI ACCTGC 1 cut(s) 460
BspPI GGATC 8 cut(s) 117, 130, 214, 363, 376, 468, 743, 775
BspT104I TTCGAA 1 cut(s) 92
BspTI CTTAAG 1 cut(s) 809
BssMI GATC 6 cut(s) 122, 206, 368, 473, 748, 767
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 2 cut(s) 437, 587
Bst6I CTCTTC 1 cut(s) 35
BstAFI CTTAAG 1 cut(s) 809
BstAPI GCANNNNNTGC 1 cut(s) 551
BstBI TTCGAA 1 cut(s) 92
BstDEI CTNAG 3 cut(s) 202, 248, 389
BstENI CCTNNNNNAGG 1 cut(s) 677
BstF5I GGATG 3 cut(s) 694, 748, 876
BstKTI GATC 6 cut(s) 125, 209, 371, 476, 751, 770
BstMAI GTCTC 2 cut(s) 390, 635
BstMBI GATC 6 cut(s) 122, 206, 368, 473, 748, 767
BstMWI GCNNNNNNNGC 3 cut(s) 20, 63, 551
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 498
BstV2I GAAGAC 2 cut(s) 162, 491
BstX2I RGATCY 5 cut(s) 122, 206, 368, 473, 767
BstYI RGATCY 5 cut(s) 122, 206, 368, 473, 767
BsuI GTATCC 1 cut(s) 534
BtsCI GGATG 3 cut(s) 694, 748, 876
BtsIMutI CAGTG 2 cut(s) 442, 547
BveI ACCTGC 1 cut(s) 460
CsiI ACCWGGT 1 cut(s) 498
CviAII CATG 3 cut(s) 253, 613, 916
DdeI CTNAG 3 cut(s) 202, 248, 389
DpnI GATC 6 cut(s) 124, 208, 370, 475, 750, 769
DpnII GATC 6 cut(s) 122, 206, 368, 473, 748, 767
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco32I GATATC 1 cut(s) 866
Eco57I CTGAAG 1 cut(s) 523
EcoNI CCTNNNNNAGG 1 cut(s) 677
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 498
EcoRV GATATC 1 cut(s) 866
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 256, 616, 919
FaiI YATR 8 cut(s) 192, 254, 534, 614, 773, 775, 888, 917
FaqI GGGAC 1 cut(s) 596
FatI CATG 3 cut(s) 252, 612, 915
FauI CCCGC 2 cut(s) 16, 282
FokI GGATG 3 cut(s) 701, 755, 883
FspBI CTAG 2 cut(s) 471, 704
GsaI CCCAGC 1 cut(s) 583
GsuI CTGGAG 1 cut(s) 299
HapII CCGG 3 cut(s) 366, 481, 527
Hin1II CATG 3 cut(s) 256, 616, 919
HindIII AAGCTT 1 cut(s) 345
HinfI GANTC 4 cut(s) 310, 406, 440, 737
HpaII CCGG 3 cut(s) 366, 481, 527
HphI GGTGA 1 cut(s) 441
Hpy166II GTNNAC 1 cut(s) 721
Hpy188I TCNGA 7 cut(s) 111, 157, 317, 373, 433, 742, 870
Hpy188III TCNNGA 8 cut(s) 120, 204, 341, 410, 527, 734, 758, 820
Hpy8I GTNNAC 1 cut(s) 721
Hpy99I CGWCG 1 cut(s) 169
HpyCH4III ACNGT 2 cut(s) 437, 587
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 3 cut(s) 4, 357, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 63, 551
HpyF3I CTNAG 3 cut(s) 202, 248, 389
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 3 cut(s) 256, 616, 919
Kpn2I TCCGGA 1 cut(s) 526
Kzo9I GATC 6 cut(s) 122, 206, 368, 473, 748, 767
LmnI GCTCC 1 cut(s) 280
LweI GCATC 3 cut(s) 13, 632, 733
MabI ACCWGGT 1 cut(s) 498
MaeI CTAG 2 cut(s) 471, 704
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 1 cut(s) 845
MalI GATC 6 cut(s) 124, 208, 370, 475, 750, 769
MboI GATC 6 cut(s) 122, 206, 368, 473, 748, 767
MboII GAAGA 6 cut(s) 52, 162, 435, 496, 556, 905
MflI RGATCY 5 cut(s) 122, 206, 368, 473, 767
MlyI GAGTC 1 cut(s) 731
MnlI CCTC 6 cut(s) 209, 457, 565, 643, 685, 758
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 526
MseI TTAA 5 cut(s) 287, 330, 591, 810, 842
MspA1I CMGCKG 1 cut(s) 277
MspCI CTTAAG 1 cut(s) 809
MspI CCGG 3 cut(s) 366, 481, 527
MspR9I CCNGG 1 cut(s) 500
MvaI CCWGG 1 cut(s) 500
MwoI GCNNNNNNNGC 3 cut(s) 20, 63, 551
NdeII GATC 6 cut(s) 122, 206, 368, 473, 748, 767
NlaIII CATG 3 cut(s) 256, 616, 919
NlaIV GGNNCC 2 cut(s) 124, 370
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 616
NspV TTCGAA 1 cut(s) 92
PaqCI CACCTGC 1 cut(s) 460
PciI ACATGT 1 cut(s) 612
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PfeI GAWTC 3 cut(s) 310, 406, 440
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PscI ACATGT 1 cut(s) 612
PsiI TTATAA 1 cut(s) 192
Psp1406I AACGTT 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 498
PspFI CCCAGC 1 cut(s) 579
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 124, 370
PsuI RGATCY 5 cut(s) 122, 206, 368, 473, 767
SaqAI TTAA 5 cut(s) 287, 330, 591, 810, 842
Sau3AI GATC 6 cut(s) 122, 206, 368, 473, 748, 767
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 1 cut(s) 500
SetI ASST 9 cut(s) 118, 349, 454, 501, 581, 611, 696, 728, 827
SexAI ACCWGGT 1 cut(s) 498
SfaNI GCATC 3 cut(s) 13, 632, 733
SfuI TTCGAA 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 758, 809
SmoI CTYRAG 2 cut(s) 758, 809
SsiI CCGC 5 cut(s) 23, 64, 275, 707, 883
SspI AATATT 1 cut(s) 89
SspMI CTAG 2 cut(s) 471, 704
StyD4I CCNGG 1 cut(s) 498
TaaI ACNGT 2 cut(s) 437, 587
TaiI ACGT 1 cut(s) 118
TaqI TCGA 3 cut(s) 92, 238, 909
TfiI GAWTC 3 cut(s) 310, 406, 440
Tru1I TTAA 5 cut(s) 287, 330, 591, 810, 842
Tru9I TTAA 5 cut(s) 287, 330, 591, 810, 842
TscAI CASTG 2 cut(s) 442, 547
TspDTI ATGAA 2 cut(s) 269, 906
TspGWI ACGGA 2 cut(s) 741, 818
TspRI CASTG 2 cut(s) 442, 547
Vha464I CTTAAG 1 cut(s) 809
XagI CCTNNNNNAGG 1 cut(s) 677
XapI RAATTY 4 cut(s) 79, 94, 458, 903
XceI RCATGY 1 cut(s) 616
XspI CTAG 2 cut(s) 471, 704
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.